PATENT ABSTRACTS

surrounds the first one and contains the regula- tion devices, and a cooling block containing the nutritive medium supply reservoirs. The auto- matic device is controlled by a microprocessor. The device of the present invention may be applied to the analysis and cloning of cellular cultures as well as for bacteriological analysis.

251

promoter and regulatory regions which control expression and secretion of proteases in a bacil- lus operably liked to a DNA sequence encoding the amino acid sequence of a polypeptide. The expression vector is particularly useful in the production of B. amyloliquefaciens proteases or other heterologous proteins in B. subtilis.

4801536

M E T H O D F O R P R O D U C I N G H E T E R O L O G O U S P R O T E I N S

Mark Stahl, Edward R LaVallie assigned to Genetics Institute Inc

This invention concerns a method for producing a heterologous protein in a bacterial host cell such that the protein is exported from the host cell into the culture medium. The method in- volves culturing in a bacterial culture medium a genetically engineered bacterial strain con- taining a fusion DNA sequence comprising a first nucleotide sequence encoding at least an N- terminal portion of a flagellin protein and a second nucleotide sequence encoding the hetero- logous protein. The first nucleotide sequence is linked via its 3' terminus to the 5' terminus of the second nucleotide sequence, and the fusion DNA sequence is itself linked to an expression control sequence. In certain embodiments the first and second nucleotide sequences are linked by means of a linking nucleotide sequence encoding a selectively cleavable polypeptide, In those embodiments the resulting exported fusion protein will contain a selectively cleavable site at which the fusion protein may be selectively cleaved by chemical or enzymatic methods to produce the heterologous protein encoded for by the second nucleotide sequence of the fusion DNA sequence. The heterologous protein may then be separately recovered from any poly- peptide fragment of flagellin or other pro- teinaceous material.

4801542

E X P R E S S I O N O F B I O L O G I C A L L Y A C T I V E P D G F A N A L O G S I N

E U C A R Y O T I C C E L L S

Mark J Murray, James D Kelly assigned to ZymoGenetics Inc

Biologically active PDGF analogs expressed in eucaryotic cells are disclosed. The analogs are produced by yeast strains transformed with an extrachromosomal element composed of a strong transcriptional promoter directing the ex- pression of a gene which encodes a protein having substantially the same biological activity as PDGF. Suitable genes include the v-sis gene or a derivative of the v-sis gene of simian sar- coma virus or portions thereof, or the human cDNA gene for PDGF or portions thereof. In particular, DNA sequences encoding poly- peptides substantially homologous to the B chain of PDGF are preferred. A secretory signal sequence may be provided upstream of the gene, enabling secretion of the gene product from the host cell. Mitogenic activity is one of the bio- logical activities possessed by these PDGF analogs, making them useful in promoting the growth of mammalian cells.

4801685

4801537

V E C T O R F O R E X P R E S S I O N O F P O L Y P E P T I D E S I N B A C I L L I

Vasantha Nagarajan, Craig S Rhodes, Carl D B Banner assigned to Genex Corporation

A replicable plasmidic expression vector, capable of high levels of expression and secretion of polypeptides in a bacillus is disclosed. The vector contains a DNA sequence comprising the

M I C R O B I A L P R O D U C T I O N O F M A T U R E H U M A N L E U K O C Y T E

I N T E R F E R O N K A N D L

David V Goeddel, Sidney Pestka assigned to Hoffmann-La Roche Inc; Genentech Inc

This invention relates to the microbial produc- tion, via recombinant DNA technology, of new human leukocyte interferons, Le IF-K and Le IF-L for use in the treatment of viral and neoplastic diseases, and to the means and end products of such production.