Enzymes and Enzyme Systems

4649107

P H E N Y L E T H A N O L A M I N E N - M E T H Y L T R A N S F E R A S E B A S E D

R A D I O E N Z Y M A T I C A S S A Y F O R N O R E P H I N E P H R I N E

Ronald R Bowsher, David P Henry assigned to Eli Lilly and Company

The present invention provides an improved method for quantifying endogenous norepinephrine employing a phenylethanolamine N-methyltransferase based radioenzymatic assay.

4649108

A L P H A A M Y L A S E A S S A Y

Henry E Blair assigned to Genzyme Corporation

Method of measuring the amount of £60 - amylase in a liquid sample, including the steps of providing an oligosaccharide substrate for + 60 - amylase, the substrate being characterized in that it contains at least 3 glucose units, its reducing-end glucose unit is bonded, via a bond cleavable by +60 - or +62 -glucosidase, to a label which exhibits an optically measurable change upon cleavage of the bond, and its ter- minal glucose unit is bonded to a blocking sub- stituent which inhibits cleavage by exo-enzymes of the bond between the terminal glucose unit and the adjacent glucose unit; contacting the sample with the oligosaccharide substrate and with a first exo-enzyme capable of cleaving the bond between the reducing-end glucose unit and the label, and measuring the optically measurable change as a measure of +60 - amylase in the sample.

4649116

M A G N E T I C M E A N S F O R W I T H D R A W I N G M A G N E T I C G E L B E A D S F R O M A N A S S A Y F L U I D

Jean Daty, Andre£0e+0 Assimon, La Chapelle en Serval, France assigned to Institut Pasteur

Magnetic device for withdrawing magnetic gel beads or the like from a biological fluid and transferring them to an immunoenzymatic assay medium. This device includes a housing of in- sulating material, particularly of plastic material, into which is inserted a magnet and over the height of which is formed a traversing orifice in which is housed a rod formed of a lower portion of magnetizable material and an upper portion of non-magnetizable material on which is mounted a return spring, between a stop washer fixed in the vicinity of the upper end of the rod and the upper surface of the housing. The device can advantageously be included in a ready-for-use kit for carrying out immuno- enzymatic assays.

4650751

P R O T E C T E D B I N D I N G A S S A Y A V O I D I N G N O N - S P E C I F I C P R O T E I N I N T E R F E R E N C E

Richard C Siegel, Christina S Marx assigned to Technicon Instruments Corporation

A specific binding enzyme-resistant ligand assay test material, which material comprises (a) a solid phase incorporated with one partner of a specific binding pair comprising said ligand or a binding analog thereof and a specific binding

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