PATENT ABSTRACTS

A method for the mass production of interferon, wherein cultured cells are brought into contact with at least one polyhydric alcohol, thereby ac- hieving a remarkable increase in the production of interferon from the cultured cells.

4550019

M A N U F A C T U R E A N D U S E O F F O W L E G G A N T I B O D I E S

Alfred Poison, Camps Bay, South Africa as- signed to South Africa Inventions Development Corporation

Immunological preparations are prepared by immunizing hens with an immunogen having a molecular or particle weight of at least 30,000, to a stage ofhyper-immnnization at which there oc- curs a plateau-like levelling-off of the antibody content of the serum. The immunogenicity of the immunogen can be enhanced by enlarging the immunogen particle mass. The eggs of the im- munized hens are collected, the yolk is separated from the eggs, followed by separation of the lipid content of the yolk. The antibodies in the egg yolk are then rendered indispersable with the aid of a water-soluble linear filamentary non- charged polymer precipitant such as PEG and the indispersable antibodies are recovered. This precipitation of antibodies is advantageously preceded by a precipitation ofcaseinaceous pro- teins, lipid and yolk particles at lower polymer concentrations. Selected antibodies (lgY or lgG) can be concentrated by pH-controlled fractional precipitation with PEG. The immunological pre- parations are useful for micro-assays and in ap- propriate cases also for the treatment or prophylaxis of pathological conditions.

4550081

N O N - R E V E R T I N G S A L M O N E L L A

Bruce A D Stocker assigned to The Board of Trustees of The Leland Stanford Jr University

Salmonella typhimurium strain SLI479, ATCC No. 39183 and Salmonella dublin strain SLI438, ATCC No. 39184 are provided which are useful as live vaccines. The vaccines are prepared by in- troducing at least one modification in a gene in- volved in at least one, normally at least two, biosynthetic pathways, involving the production of products which are unlikely to be found in the disease susceptible host. The modification results in a gene change which cannot be repaired by a single step e.g. polynucleotide deletions and inversions. Where the aro gene suffers such a change, the resultant auxotrophic mutants re- quired aromatic amino acids, p-aminobenzoic

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acid and enterochelin or a highly concentrated source of absorbable iron. The auxotrophic mutations have substantially reduced or none- xistent virulence, while retaining the desired immunogenicity to initiate the immunogenic response.

4550086

M O N O C L O N A L A N T I B O D I E S T H A T R E C O G N I Z E H U M A N T

C E L L S

Ellis Reinherz, Stuart F Sehlossman. Stefan C Meuer assigned to Dana-Farber Cancer In- stitute lnc

A monodonal antibody which specifically binds to the surface recognition structure of a pre- determined mature human T cell done, which recognition structure renders the clone capable of acting as causative agent in a predetermined autoimmune disease, the monoclonal antibody being capable of specifically binding to the recognition structure of the clone to inhibit the ability of the clone to act as a causative agent in the predetermined autoimmune disease.

4551271

P U R I F I C A T I O N O F I N T E R F E R O N B Y M E T A L C H E L A T E C H R O M A T O G R A P H Y

Erich Hochuli, Arisdorf, Switzerland assigned to Hoffmann-La Roche Inc

The purification of solutions of recombinant interferons is achieved by chromatography on metal chelate resins of the formula See Patent for Chemical Structure in which Me represents cop- per or nickel.

4551288

P R O C E S S E S F O R T H E P R E P A R A T I O N O F L I P O S O M E

D R U G D E L I V E R Y S Y S T E M S

Lawrence A Kelly assigned to Sandoz Inc

Process and apparatus for the production of medicament containing liposomes, comprising an aliphatic lipid-sterol-water lamellae, wherein a clear, mixed micellar medicament-detergent-