10 15

1

1 11 1

13:00 13:20

13:20 13:50

13:50 14:20

14:20 14:50

14:50 15:10

15:10 15:40 Saccharomyces cerevisiae

15:40 16:45

17:00

(Aspergillus oryzae )

DNAchip 5513 ( 1)

RIB40 ( )13 RIB40

RIB128 RIBOIS01 RIB915 4

1313

9 Illumina HiseqXRIB40

5

0.1

(Log)

1.0

RIB40

1. DNAchip

SNPde novo

RIB40500 bp

RIB40mapping8

SNP SNPRIB40 RIB1172 SNP 6 RIB143

1816 bp

6

2

RIB40 backbone0~8 backbone

1

( 2,3) aflR aflJ

2aflR aflJ

1 aflR, aflJ

132

2

%

52-5S-38

BCP pH

Aspergillus luchuensis mut. kawachiipH

Aspergillus oryzae

CtpA YhmA 1) ctpA yhmA

CoACexA

cexACexA

2)

LaeA LaeA

laeA 590658

cexA laeA cexA

PCR

laeA cexAH3K4me3

H3K9me3LaeA cexA cexA

1 Kadooka, C. et al., Appl. Environ. Microbiol., 85, e03136-18 (2019). 2 Kadooka, C. et al., bioRxiv, 748426 (2019).

CtpA

YhmA

CoA

CexA?

Saccharomyces cerevisiae

20 DNA

Saccharomyces cerevisiae

PCR

1

Sugiyama et al., 2005 2

YAC

, 2010

1.

Sugiyama et al., 2008

Natesuntorn et al., 2015

CRISPR-Cas9

Sasano et al., 2016

Kaboli et al., 2016

Sugiyama, M., Ikushima, S., Nakazawa, T., Kaneko, Y., Harashima, S.: PCR-mediated repeated

chromosome splitting in Saccharomyces cerevisiae. BioTechniques, 38(6):909-914 (2005).

Sugiyama, M., Nakazawa, T., Murakami, K., Sumiya, T., Nakamura, A., Kaneko, Y., Nishizawa, M.,

Harashima, S.: PCR-mediated one-step deletion of targeted chromosomal regions in haploid

Saccharomyces cerevisiae. Appl. Microbiol. Biotechnol., 80(3):545-553 (2008). : . , 88(2):54-59 (2010).

Natesuntorn, W., Iwami, K., Matsubara, Y., Sasano, Y., Sugiyama, M., Kaneko, Y., Harashima, S.:

Genome-wide construction of a series of designed segmental aneuploids in Saccharomyces

cerevisiae. Scientific Reports, 5:12510 (2015).

Sasano, Y., Nagasawa. K., Kaboli. S., Sugiyama, M., Harashima, S.: CRISPR-PCS: a powerful new

approach to inducing multiple chromosome splitting in Saccharomyces cerevisiae. Scientific Reports,

6:30278 (2016).

Kaboli, S., Miyamoto, T., Sunada, K., Sasano, Y., Sugiyama, M., Harashima, S.: Improved stress

resistance and ethanol production by segmental haploidization of the diploid genome in

Saccharomyces cerevisiae. J. Biosci. Bioeng., 121(6):638-644 (2016).

Saccharomyces cerevisiae

7 K7Ime1

Ime1 K7Nakazawa et al., JBB, 73, 265, 1992

K7 Ime1Cln3

Nakazawa et al., JBB, 110, 1, 2010K7

Shimoi et al., JBB, 127, 190, 2019

K7 100 3 8 2K7 4 DNA

K6 K7 K9 K105 CAN1 URA3

CAN1/can1 URA3/ura3 100 38 K7 4 DNA

K7K6 K7 K9 K10 K7

K7K7

K7 UT-1ura3 /ura3 trp1 /trp1 URA3 CAN1

ADE2 TRP1 HIS3ade2 his3

UTCAH-3 YCp

canavanine 5-fluoroanthranilic acidADE2 HIS3 15

can1 trp1 ADE2 HIS3

can1 trp1 ade2 HIS3 1

UTCAH-3

SPO11SPO11 UTCAH-3

K7 SPO11K7SPO11 spo11 /spo11

K7 Spo11

SPO11 K7SPO11

K7 RIM15

RIM15 K7RIM15 RIM15

SPO11 RIM15

表紙要旨（齊藤）講演要旨（中島）要旨（二神泰基）要旨（杉山峰崇）要旨（下飯）