340 PATENT ABSTRACTS

range, and little sensitivity to heavy metal ion in- hibition. The enzyme may be immobilized on an organic macroporous polymer or in an alginate gel.

4396602

B L O O D G L U C O S E L E V E L L O W E R I N G A G E N T S

Akira Endo. Tokyo, Japan assigned to Asahi Kasei Kogyo Kabushiki Kaisha

A method of lowering the blood glucose level in mammals and a blood glucose level-lowering a- gent are described. The method comprises ad- ministering an enzyme capable of synthesizing sparingly-digestible saccharides from easily- digestible saccharides. The blood glucose level- lowering agent comprises the enzyme capable of synthesizing sparingly-digestible saccharides from easily-digestible saccharides and a glucosidase-inhibiting agent.

4395487

M E T H O D F O R A S S A Y O F A L P H A - A M Y L A S E A C T I V I T Y

Narimas Saito, Tatsuo Horiuchi, Noda, Japan assigned to Noda Institute for Scientific Research

A method for the assay of alpha-amylase ac- tivity, which compriSeS adding an alpha- amylase-containing sample to maltohexaitol or maltohexaonic acid used as substrate, reacting, at the same time or subsequent to the addition, alpha-glucosidase with the resulting mixture, and determining the reaction product to assay the alpha-amylase activity.

4394450

M E T H O D F O R P U R I F I C A T I O N O F U R I C A S E

David A Brock, Surendra K Gupta assigned to Miles Laboratories Inc

A method is provided for purifying uricase by decreasing the amount of active catalase present. The method involves adjusting the pH of a catalase-containing uricase preparation to a pH in the range of about I I to 13 to inactivate the catalase and recovering a uricase preparation substantially free of active catalase.

4394449

S T A B I L I Z A T I O N O F C O E N Z Y M E S I N A Q U E O U S

S O L U T I O N

Ivan E Modrovich

A stabilized aqueous coenzyme solution is dis- closed for use in the clinical assay of a selected biological constituent through the enzymic reac- tion of an enzyme with a selective substrate for the enzyme and a coenzyme. The aqueous coen- zyme solution comprises at least one first enzyme and a first substrate for selectively reacting with the fir.st enzyme in the assay, a coenzyme for interacting with the first enzyme and first subs- trate in the assay which coenzyme degrades for- ming a coenzyme conversion product, and at least one second enzyme and second substrate selective for the second enzyme which react with the coenzyme conversion product to regenerate the coenzyme.

4394445

E N Z Y M A T I C G L Y C E R I D E H Y D R O L Y S I S

Paul T Nix, Janet M Santoro, Joyce E Stephens

A new stable combination of Rhizopus arrhizus lipase and Pseudomonas fluorescens lipase for