4.14 to investigate the effect of iaa growth regulator on plant tissue
DESCRIPTION
4.14 To investigate the effect of IAA growth regulator on plant tissue. Before you start get or make a copy of this Table of results for Roots, and …. Before you start get or make a copy of this Table of results for Shoots. Step 1. - PowerPoint PPT PresentationTRANSCRIPT
4.14 To investigate the effect of IAA growth regulator on plant tissue
Before you start get or make a copy of this
Table of results for Roots, and …Dish Concentration of IAA Length of Roots (mm)
Total Length
Average
Length
% increase
or
(mg/litre) (ppm) Seed 1 Seed 2 Seed 3 Seed 4 Seed 5 Seed 6 Seed 7 Seed 8 (mm) (mm) decrease
A 100.000000 102 0
B 10.000000 101 0
C 1.000000 100 0
D 0.100000 10-1 0
E 0.010000 10-2 0
F 0.001000 10-3 0
G 0.000100 10-4 0
H 0.000010 10-5 0
I 0.000001 10-6 0
J (Control) 0 0 0
Before you start get or make a copy of this
Table of results for ShootsDish Concentration of IAA Length of Shoots (mm)
Total Length
Average
Length
% increase
or
(mg/litre) (ppm) Seed 1 Seed 2 Seed 3 Seed 4 Seed 5 Seed 6 Seed 7 Seed 8 (mm) (mm) decrease
A 100.000000 102 0
B 10.000000 101 0
C 1.000000 100 0
D 0.100000 10-1 0
E 0.010000 10-2 0
F 0.001000 10-3 0
G 0.000100 10-4 0
H 0.000010 10-5 0
I 0.000001 10-6 0
J (Control) 0 0 0
Step 1
Label eight petri dishes 102, 101, 1, 10-1, 10-2, 10-3, 10-4 and control.
Step 2
Add 10 ml of the IAA solution to the dish labelled 102.
Step 3
Using a different syringe, add 9 ml of water to each of the other dishes.
Step 4
Using a dropper, remove 1ml of solution from the 102 dish and add it to the 101 dish.
Step 5
Stir the solution using the dropper. Repeat for the remainder of the dishes (1, 10-1, 10-2, 10-3 and 10-4), using a different dropper each time.
Step 6
Discard 1ml of liquid from the dish labelled 10-4.
Step 7
Place an acetate grid on the lid of each petri dish. Place 5 radish
seeds along the cross lines.
Step 8
Place filter paper on top of the seeds. Spread cotton wool on top of the filter paper.
Step 9
Put the base of each petri dish on its lid. Secure the dishes and incubate vertically at 25°C for 2-3 days.
Step 10
Measure the lengths of roots and shoots of each seed using the acetate grid.
Results & calculations
Calculate the average length of the stems and roots at
each concentration
and the percentage stimulation or inhibition.
Chart and graph the results
Root results
Dish Concentration of IAA Length of Roots (mm)Total
Length
Average
Length
% increase
or
(mg/litre) (ppm) Seed 1 Seed 2 Seed 3 Seed 4 Seed 5 Seed 6 Seed 7 Seed 8 (mm) (mm) decrease
A 100.000000 102 0 #DIV/0! #DIV/0!
B 10.000000 101 0 #DIV/0! #DIV/0!
C 1.000000 100 0 #DIV/0! #DIV/0!
D 0.100000 10-1 0 #DIV/0! #DIV/0!
E 0.010000 10-2 0 #DIV/0! #DIV/0!
F 0.001000 10-3 0 #DIV/0! #DIV/0!
G 0.000100 10-4 0 #DIV/0! #DIV/0!
H 0.000010 10-5 0 #DIV/0! #DIV/0!
I 0.000001 10-6 0 #DIV/0! #DIV/0!
J (Control) 0 0 0 #DIV/0! #DIV/0!
Shoot results
Dish Concentration of IAA Length of Shoots (mm)Total
Length
Average
Length
% increase
or
(mg/litre) (ppm) Seed 1 Seed 2 Seed 3 Seed 4 Seed 5 Seed 6 Seed 7 Seed 8 (mm) (mm) decrease
A 100.000000 102 0 #DIV/0! #DIV/0!
B 10.000000 101 0 #DIV/0! #DIV/0!
C 1.000000 100 0 #DIV/0! #DIV/0!
D 0.100000 10-1 0 #DIV/0! #DIV/0!
E 0.010000 10-2 0 #DIV/0! #DIV/0!
F 0.001000 10-3 0 #DIV/0! #DIV/0!
G 0.000100 10-4 0 #DIV/0! #DIV/0!
H 0.000010 10-5 0 #DIV/0! #DIV/0!
I 0.000001 10-6 0 #DIV/0! #DIV/0!
J (Control) 0 0 0 #DIV/0! #DIV/0!
Expected graphEffect of varying auxin concentrations on Root and Shoot Development
-150
-100
-50
0
50
100
150
200
0 1 2 3 4 5 6 7 8 9 10
Auxin concentration (parts per million)
Per
cen
tag
e st
imu
lati
on
of
Gro
wth
In
hib
itio
n
Sti
mu
lati
on
Root Shoot
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