[41-49] [83-93] c-tni tni19c7 ctnt-specific mabs and their …€¦ · blood samples than in the...
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control group TnAAbs control group TnAAbs control group TnAAbs0
102030405060708090
100110120130140150160
p=0.81
p=0.095
cTnII-C complex
cT
nI
reco
ve
ry (
%)
cTn complex
p<0.05
μ
[41-60] [54-73] [67-86] [80-99] [119-138][132-152][145-164][171-190][184-203][223-242][275-288]
0
10
20
30
40
50
60
70
80
90
100
110
120
130
TnT199 TnT7E7 TnT105 TnT2 TnT1A11 TnT9 TnT122TnT8 TnT175 TnT25
cTnT-specific mAbs and their epitope specificity, aar
cT
nT
re
co
ve
ry (
%)
TnAAbs plasma
Control plasma
TnT124
● ■
1. TnAAbs are not specific to the cTnI per se but instead to the structural
epitopes formed by cTnI and cTnT polypeptide chains
2. The effect of TnAAbs on cTnI measurements is less pronounced in AMI
blood samples than in the samples with spiked ternary cTnI-cTnT-TnC
complex
3. The negative effect of TnAAbs on cTnI measurements is more
noticeable in the early AMI samples (first few hours after the event) rather
than in the late AMI samples
4-9h 10-19h 22-36h0
10
20
30
40
50
60
70
80
90
100
cT
nI re
covery
(%
)
Hours after onset of AMI symptoms
Standard I-C
complex recovery
Standard cTnI-cTnT-TnC
complex recovery
μ
40 45 50 55 60 65 70 75 80 85 90 95
Elution volume (ml)
CP
S
Normal plasma
TnAAbs-plasma
2000
3000
4000
5000
6000
7000
8000
9000
10000
11000
12000cTnI-TnC complex in buffer
40 45 50 55 60 65 70 75 80 85 90 95
1000
2000
3000
4000
5000
6000
7000
8000
9000
10000
11000
CP
S
Elution volume (ml)
TnAAbs-plasma
Normal plasma
cTnT in buffer
40 45 50 55 60 65 70 75 80 85 90 95
Normal plasma
TnAAbs-plasma
Elution volume (ml)
CP
S
2000
3000
4000
5000
6000
7000
8000
9000
10000
11000
12000
cTnI-cTnT-TnC complex in bufferBA C
TnI19C7
[41-49]
TnI560
[83-93]
C-TnI
N’C-TnC
N-TnT
C-TnT
N-TnC
TnT7E7
[223-242]
TnAAbs putative epitope
within IT arm
T1-TnT