*$#4 · *this study is supported by scientific activities support program of eskişehir osmangazi...
TRANSCRIPT
International Congress on Applied
Biological SciencesNovember 08-12 I Sarajevo
5TH
w w w . i c a b s . g e n . t r
ICABS 2019
SARAJEVO
Book of Abstracts
1
THE EFFECT OF BERBERINE IN THE PREVENTION AND / OR TREATMENT
OF TESTICULAR INJURY DUE TO CYCLOPHOSPHAMIDE IN RATS
MAHMUT ÖZDEMIR1, HAKAN ALTUNTAŞ1, SEMRA YIĞITASLAN1, VAROL ŞAHINTÜRK2
1ESKIŞEHIR OSMANGAZI UNIVERSITY, FACULTY OF MEDICINE, DEPT. OF PHARMACOLOGY
2ESKIŞEHIR OSMANGAZI UNIVERSITY, FACULTY OF MEDICINE, DEPT. OF HISTOLOGY AND
EMBRYOLOGY
Abstract
We aimed to investigate the preventive and/or therapeutic effect of berberine on cyclophosphamide-induced
testicular injury. After body weight was measured, a total of 40 rats were randomly divided into 5 groups of 8.
The animals in the control group received oral SF for 14 days. The rats in the CP, ber75, ber150 and ber300
groups were given orally, SF, 75, 150 and 300 mg / kg berberine for 14 days, respectively, and all animals were
injected i.p. 200 mg / kg CP on day 8. 24 hours after the last dose, the animals were weighed again and the
testicular tissues were removed under general anesthesia and then euthanized. After weights were measured,
testicular tissues were used for histological examination, immunohistochemical apoptosis evaluation and
homogenate preparation. Oxidative stress and cytokine levels in testicular homogenate were evaluated. While
CP had no significant effect on body and testicular weights, berberine reduced the body weight in a dose-
dependent manner. Although there was no significant change in oxidative stress markers and cytokine levels,
degenerative changes and apoptotic findings were found in the CP group and these were improved by dose-
dependent treatment with berberine. These effects of berberine may be due to their anti-degenerative and anti-
apoptotic activity rather than being mediated by oxidative stress and inflammation.
In conclusion, our findings suggest that berberine treatment may be useful in the prevention / treatment of CP-
dependent testicular injury.
Keywords: Berberine, Cyclophosphamide, Rat, Testicular Injury
*This study is supported by Scientific Activities Support Program of Eskişehir Osmangazi University
2
INVESTIGATION OF FENTANYL PANCREATIC CANCER CELL PROLIFERATION
AND CANCER STEM CELL DIFFERENTIATION
FEYZI CELIK1, TUGCE DURAN2
1DEPARTMENT OF ANESTHESIOLOGY, FACULTY OF MEDICINE, DICLE UNIVERSITY,
DIYARBAKIR, TURKEY
2DEPARTMENT OF MEDICAL GENETICS, FACULTY OF MEDICINE, KTO KARATAY UNIVERSITY,
KONYA, TURKEY
Abstract
Pancreatic cancer is one of the most aggressive cancer due to the late diagnosis and failure to respond to the
treatment despite advances in tumor biology and the development of new cancer therapeutic strategies. It has
been reported that these characteristics of pancreatic cancer originate from cancer stem cells within the tumor
mass. It has also been reported that the Fentanyl and some other mu-opioid receptors are involved. In this study,
we determined the effect of Fentanyl on PANC-1 cells, by assessing the gene expression of cancer stem cell
marker genes (Nanog, Oct4, and Sox2) and apoptosis-related genes (BAD, Bax, Bcl-2, and p53) by Quantitative
RealTime PCR. The number of cancer stem cells was determined by flow cytometry. The results of our study
showed that Fentanyl administration decreased the number of cancer and cancer stem cells in the PANC-1 cell
population, decreased the gene expression of stem cell marker and increased the expression of apoptosis-related
genes. These results indicate that Fentanyl, which is used routinely in the pain palliation of pancreatic cancer,
can be considered as an option in the treatment of pancreatic cancer.
Keywords: Apoptosis, Cancer Stem Cell, Fentanyl, Pancreatic Cancer
*This project is supported by Dicle University Scientific Research Projects Coordination Office with TIP.18.016
project numbers
3
ANTIFUNGAL AND ANTI-BIOFILM ACTIVITY OF NEW PROPANE SULFONYL
HYDRAZONE AGAINST CANDIDA ISOLATES
MERVE AYDIN1, ALI OZTURK2, UMMUHAN OZDEMIR OZMEN3
1DEPARTMENT OF MEDICAL MICROBIOLOGY, FACULTY OF MEDICINE, KTO KARATAY
UNIVERSITY, KONYA, TURKEY
2DEPARTMENT OF MEDICAL MICROBIOLOGY, FACULTY OF MEDICINE, NIĞDE ÖMER
HALISDEMIR UNIVERSITY, NIĞDE, TURKEY
3DEPARTMENT OF CHEMISTRY, FACULTY OF SCIENCE, GAZI UNIVERSITY, ANKARA, TURKEY
Abstract
Candidiasis is the most frequently encountered fungal disease that ranges from mild mucosal infections to
serious candidaemia and disseminated candidiasis. There are only limited classes of antifungal agents available
for physicians to combat Candida infections. In addition, biofilm cells display phenotypic traits that resulting in
enhanced resistance to antifungal drugs. Therefore, the limited option to combat fungal threat has raised the
interest in seeking alternative antifungal compounds. The aim of this study was to investigate the antifungal and
anti-biofilm activity of the new Sulfonyl hydrazones compound Anaf-Psh (3-hydroxynaphthalene-
2ethylidenepropane sulfonylhydrazone), derived from sulfonamides. The Candida species evaluated in this
study included the five reference strains and thirty-five clinical isolates belonging to a collection of fungal
strains previously established at the Gazi University Medical Mycology Laboratory. Identification was
performed by conventional methods and by biochemical characterization using the API ID32C® system
(Biomerieux) and was confirmed by the sequencing. The antifungal activity of Anaf-Psh was determined using a
broth microdilution method according to the European Committee on Antimicrobial Susceptibility Testing
(EUCAST) standards. The compound concentrations varied between 0.5–256 µg/ml. The anti-biofilm effect of
the synthesized compound was evaluated in 96-well polystyrene flat-bottom microplates. The compound was
found to be effective against all tested Candida strains with MIC ranging from 8-64 µg/ml and also inhibit
biofilm formation in tested isolates. As a conclusion, it's in vitro antifungal and anti-biofilm properties, this new
compound Anaf-Psh is a promising new agent for the control and treatment of Candida infections.
Keywords: Anti-Biofilm Activity, Antifungal Activity, Candida, Sulfonyl Hydrazones
*This research received no specific Grant from any funding agency in the public, commercial, or not-for-profit
sectors
4
EVALUATION OF NUTRITIONAL HABITS OF ATHLETES BETWEEN 18-40
YEARS
MERVE PEHLİVAN, FATMANUR TERCAN
İSTANBUL MEDIPOL ÜNIVERSITESI
Abstract
Aim: This study aims to investigate dietary habits and the prevalence rate of disordered eating behaviors of 107
athletes (age, 18-40 years), using questionnaire. Material and method: Participants (50 males, 57 females)
completed a questionnaire including anthropometric measurements, dietary habits questions and eating attitude
test (EAT-40). The analysis of acquired datas was done using SPSS (version 21.0) and significance for
statistical datas was selected being p<0.05.
Results: The mean BMI of male athletes was 24.1±2,9 kg/m2, while the mean of female athletes was 21.04±2,8
kg/m2 and both groups were within normal BMI ranges. While 58.9% of the athletes stated that they skipped
meals, the most skipped meal was breakfast with 42.9%. It was observed that most athletes consume meat
(%94,4) and dairy products (%96,3). 12.3% of female athletes and 14.0% of male athletes consume sports
drinks; energy drink consumption for male and female athletes is 24.6% and 26.0%, respectively. Most of the
male and female athletes (80.7% and 86.0%, respectively) were not predisposed to eating disorders. There was
no significant relationship between athletes' eating attitude test scores and BMI, body weight, type of sport and
regular breakfast (p>0,05). Conclusion: Having healthy eating habits is very important for athletes to maximize
their performance. Regular education should be given to athletes to gain healthy eating habits and protect them
from eating disorders.
Keywords: Dietary Habits, Athlete, Eating Attitude Test
*
5
EFFECT OF CYSTINE AND BORON ON SPERM QUALITY AND MEMBRANE
INTEGRITY AFTER FROZEN-THAWED OF PIRLAK RAM SEMEN
FATIH AVDATEK, DENIZ YENİ
AFYON KOCATEPE UNIVERSITY
Abstract
Non-essential means that our bodies produce an amino acid, even if we do not get it from the food we eat. Non-
essential amino acids include: alanine, arginine, asparagine, aspartic acid, cysteine, glutamic acid, glutamine,
glycine, proline, serine, and tyrosine. The aim of this study is to investigate the effects of cystein and boron
adding to extender on ram sperm motility, membran integrity and viability test (HOST/E), after frozen-thawed
of ram semen. Ejaculates were collected from five Pirlak rams using an artificial vagina ones a week and this
process was repeated six times. Ejaculates were split into three aliquots and diluted to a final concentration of
150x106 spermatozoa/ml with the base extender containing cystine 1 mM, boron 1 mM and no antioxidant
(control). Following dilution, the specimens were drawn in 0.25 ml different color aliquots and subjected to 3
hours of equilibration at 5 ºC, followed by freezing in 15 minutes in nitrogen vapor (~ -110 ºC), the specimens
were stored frozen in liquid nitrogen (-196 ºC) until in vitro evaluation. After being stored for six months, the
aliquots were individually thawed in a water bath at 37 ° C for 20 s for microscopic evaluation.
Sperm motility was increased, abnormal sperm rate were decreased significantly (p<0.05) in boron, and
membrane integrity were increased significantly (p < 0.05) in cystine when compared to the control group.
İn conclusion boron and cystine improve spermatological parmeters and membrane integrity in breeding season.
Keywords: Boron, Cystine, Membrane Integrity, Ram Sperm
*
6
EFFECT OF DIFFERENT DURATION OF INTRAVAGINAL SPONGE
TREATMENTS ON THE SOME REPRODUCTIVE PARAMATERS IN THE
SAANEN GOATS
MUHAMMED KÜRŞAD BIRDANE
AFYON KOCATEPE UNIVERSITY
Abstract
The aim of this study was to investigate the effect of different duration treatments of progesterone sponges on
the some reproductive parameters in the Saanen goats at weather conditions of Afyonkarahisar/Turkey. Before
the study, ultrasonography examination was performed for reproductive disorders and total 68 multiparaus and
lactating Saanen goats were selected from herd. In July, intravaginal sponges (Chronogest®) were applicated in
all of groups within the same day for estrus synchronization. Sponges were removed on the days
7th,9th,11th,13th in the groups 1, 2, 3 ,4 respectively (in each group n=17). All group were injected with 480 lU
PMSG (Chronogest-PMSG® i.m) and 125 µg Cloprostenol (Estrumate® i.m) one day before sponge remove
day. At 24 hours after the removal of the sponges, three male goats were placed in each groups for natural
mating. Pregnancy was checked through transrectal ultrasound in dorsal recumbency position on days 25-30
after sponge remove day. The estrus and pregnancy rates were found 82% (14/17), 88% (15/17), 82% (14/17),
81% (13/16) and 57% (8/14), 73%(11/15), 64%(9/14), 53%(7/13) in the groups 1,2,3,4 respectively. Litter size
were found 1,87(15/8), 1,90(21/11), 2,0(18/9) and 1,71(12/7) in the groups. Reproductive parameters were
statistically insignificant between groups. As a result of this study, the synchronization of estrus with this
method did not important change on the reproductive performance in the Saanen goats.
Keywords: Flugestone Acetate, Lactating Goats, Pregnancy Rate, Afyonkarahisar,
*
7
POPULAR DIET PRACTICES AND KNOWLEDGE LEVELS OF ADULT
INDIVIDUALS AGED BETWEEN 18-65 YEARS
FATMA ELIF SEZER, BURCU USTAD, MUSTAFA SAFA YONTEM
ISTANBUL MEDIPOL UNIVERSITY, FACULTY OF HEALTH SCIENCES, DEPARTMENT OF
NUTRITION AND DIETETICS
Abstract
This study aimed to determine the popular diet practices and knowledge levels of adult individuals aged
between 18-65 years. The study was conducted with 300 participants (150 males, 150 females) in Istanbul. The
participants' level of knowledge about popular diets and the resources they learned were obtained through a pre-
prepared questionnaire. To evaluate the data, SPSS 22.0 statistical package program was used. It was found that
the average age was 33.2±10.4 years and 50.3% of the participants were normal, 45.7% were overweight and
obese. Popular diets followed by 24% of women, 16.7% of men. It was determined that 91.7% of women and
70.8% of men lost weight with these diets. However, 70.3% of women and 68.8% of men regained weight in the
first 6 months after quitting diet. The weight lost (7.08±5.09 kg) was found to be similar to the weight gained
after quitting diet (7.24±6.7 kg). Participants learned popular diets from internet/social media (25.8%), TV/radio
(24.8%) and their close circle (24.4%). The most well-known popular diets; Karatay diet (24.2%), sugar free
diet (14.7%) and gluten-free diet (12.5%). Popular diets are effective in losing weight in the short term, but do
not contribute to weight management by causing weight gain again in the long term. Incorrect nutrition and diet
practices in weight management should be prevented. Adequate, balanced, healthy nutrition should be provided
with the support of dietician. The negative effects of popular diets should be explained by health professionals
and the community should be raised awareness.
Keywords: Weight Management, Popular Diet, Malnutrition, Weight Loss
*
8
UNUSUAL LOCATION OF SPIROCHAETES IN LYMPH VESSELS IN BOVINE
DIGITAL DERMATITIS LESIONS
MEHMET FATIH BOZKURT, İBRAHIM DEMİRKAN
AFYON KOCATEPE UNIVERSITY, FACULTY OF VETERINARY MEDICINE
Abstract
The precise aetiology of Digital dermatitis has not been established. However, the rapid spread of disease within
affected herds and a good respond to topical antibacterial suggest that it may have an infectious cause.
Treponemes have been identified in vivo in the lesion and frequently isolated from the lesions and subsequently
cultured in vitro. The role of treponemes in its aetiology still remains uncertain. Fourty biopsies were taken from
dairy cow suffering from digital dermatitis. They placed in 10% buffered neutral formalin solution for
histopathological and spirochaetal immunohistochemical analysis. In immunohistochemical examination,
spirochaetal bacteria were detected in 38 samples. It was observed that these bacteria entered between the
keratinocytes and reached to the pars papillary dermis. They continued to proliferation and stimulate the
inflammatory reaction here. it was found to progress to the deep dermis using lymphatics. Thus, it was observed
that they formed a large and severe inflammatory reaction in the dermis. As a result; It was seen that the agents
reaching the dermis region closest to the epidermis surface by using the epithelial cells that started to be
keratinized from the superficial layer travelled to deeper dermis regions by using lymph vessels. Thus, it was
concluded that a more potent inflammatory reaction and its involvement in circulation could lead to more
complicated events such as septicemia, affect the clinical course and severity of the disease, and especially
localization within lymphatic vessels could be an important mechanism for etiopathogenesis.
Keywords: Digital Dermatitis, Treponema, Immunohistochemistry, Cattle,
*This study is supported by the BAPK of Afyon Kocatepe University with the project no 17.kariyer.211
9
THE EFFECTS OF BENTAGRAM ON THE DEVELOPMENT AND ANTIOXIDANT
PARAMETERS OF ARTHROSPIRA PLATENSIS GOMONT
ŞÜKRÜYE ER
SAKARYA UNIVERSITY
Abstract
Herbicides are chemical mixtures used to prevent, control or reduce harmful organisms. These chemicals
especially affect the primary producers that constitute the most important link of the food chain affects the
whole ecosystem. In this context, the impact of such chemicals on algae is extremely important. In this study, in
the C. vulgaris algae exposed to Bentagram, the decrease in OD560 value was observed, while the increase in
the amount of chlorophyll-a was significant for 7 days. There was no significant change on SOD, APX and GR
enzyme activities. An increase in GR enzyme activity was observed only at a concentration of 960 μg mL-1.
MDA and H2O2 amounts are one of the main by-products of lipid peroxidation, which is associated with
oxidative stress in cells. When the total MDA amounts of C. vulgaris cultures were compared due to increasing
concentrations of the Bentagram, a statistically significant decrease was observed in the concentration of only
960 μg mL-1 compared to the control. The total amount of H2O2 did not show statistically significant change
compared to the control. The antioxidant enzyme that determines the accumulation of H2O2 in cells and plays
an important role is APX (Asada, 1992). The amount of free proline decreased significantly at 960 μg mL-1,
which is the highest concentration compared to the control. Increased GR enzyme activity at the same
concentration also supports this information.
As a result, changes in biomass and antioxidant parameters were found to be related to increasing concentrations
of Bentagram.
Keywords: Bentagram, Antioxidant Enzyme, Herbicide, Arthrospira Platensis
*This study was supported by Sakarya University Scientific Research Projects (BAP) Commission Presidency
(Project No: 2018-02-09-
10
SILENCING OF EAG1 MAY EFFECTS PROLIFERATION AND APOPTOSIS
THROUGH GENE EXPRESSION OF ERK1 AND JNK IN CANCER
AHU SOYOCAK1, DIDEM TURGUT COSAN2
1ISTANBUL AYDIN UNIVERSITY MEDICAL FACULTY DEPARMENT OF MEDICAL BIOLOGY,
ISTANBUL, TURKEY
2ESKISEHIR OSMANGAZI UNIVERSITY MEDICAL FACULTY DEPARMENT OF MEDICAL
BIOLOGY, ESKISEHIR, TURKEY
Abstract
Objective: Extracellular signal-regulated protein kinase (ERK) and c-jun amino-terminal kinase (JNK) are
members of the mitogen-activated protein (MAP) kinase family, ERK is activated by growth factors to stimulate
cell growth, differentiation, and proliferation, while JNK is activated by stress-induced stimuli and mediates
apoptotic signals. It is known that voltage-gated ion channels also play an important role in these cellular
processes. Eag1 (KCNH1, Kv10.1, Ether à go-go), a member of the voltage-gated potassium channel family has
been shown to have oncogenic features and is overexpressed in many human tumors, including breast cancer.
This study aimed to determine the effect of siRNA-mediated silencing of the Eag1 potassium channel on ERK1
and JNK gene expression in strong metastatic MDA-MB-231 cells.
Materials-Methods: ERK1 and JNK gene expressions in Eag1siRNA transfected MDA-MB-231cells were
determined on the Real-Time PCR (qRT-PCR (Stratagene, Mx3000p). Gene expression levels were calculated
using the formula 2−ΔCt = 2−(Ct gen − Ct housekeeping gen).
Results: It was determined that ERK1 expression levels decreased by -1.75 fold and JNK expression level
increases 1.56 fold in Eag1 siRNA transfected MDA-MB-231 cells.
Conclusion: The silencing of the Eag1 gene may alter the signals of proliferation and cell death. Our results
suggest that this gene may be a molecular target in the formation or development of breast cancer and further
research is needed.
Keywords: Eag1, MDA-MB-231, ERK1, JNK, Gene Expression.
11
HUMAN-WILDLIFE INTERACTIONS IN YOUNG GENERATION
AWARENESS/PERCEPTION
ISIDORO DE BORTOLI, FILIPPO FAVILLI
EURAC RESEARCH
Abstract
Conflicts and their resolutions among alpine communities represent the process of intensive communication in
local societies, and how conflicts are resolved reflect a Region’s political and social structures. Often conflicts
occur at the interface between traditional, primary activities and new developing economic activities that have a
less environmentally sustainable approach towards natural resources. Nowadays the strain on sharing resources
accelerates the tip-over point towards conflicts. We noted that the presence, return and overabundance of
adaptable species in the Alpine arch, has found many stakeholders and administrators unprepared. Human
presence and expansion in Alpine territories inflict pressure on mobile wildlife species, putting them in the
middle of growing conflicts with humans, often hidden from right view of young generation.
Therefore, the educative process is needed where people wrongly understand wildlife and human activities-
related impact because of a lack of knowledge. However, such approaches are hindered by the family
background of young generation, strongly influenced by household aptitude to wildlife acceptance.
Our analysis identified different human-wildlife interactions in the Alps. The aim of the study is to educate
young generation to be aware of main human-wildlife interactions in order to prevent any conflict and to
provide them as much as possible right information. To meet these challenges, we propose an abstracted and
youthful model that adopts a social-environmental approach to show results have been achieved in interviewing
more than 1000 high school students, coming from different parts of the Alpine arch.
Keywords: Interaction, Wildlife, Young Generation, Cohexistence, Conflict
*
12
PHYSICOCHEMICAL PROPERTIES OF STIRRED YOGURT PRODUCED USING
WHEY PROTEIN, INULIN AND CREAM
EMINE MINE ÇOMAK GÖÇER, FIRUZE ERGİN AHMET KÜÇÜKÇETİN
AKDENİZ ÜNİVERSİTESİ
Abstract
Yogurt is among the most common fermented dairy products consumed all over the world. The standardization
of yogurt milk can affect the physicochemical properties of the yogurt which are important acceptance criteria
for consumers. In this study, the aim was to investigate the effect of adding whey protein, inulin and cream to
yogurt milk on the physicochemical characteristics, including the graininess and roughness, of stirred yogurt.
Yogurt milk was standardized by using whey protein, inulin or cream to obtain %15 total solids. The
standardized milk was heated at 72 °C for 15 sec and then, cooled to 42 °C. Cooled milk was inoculated with
yogurt starter culture. After inoculation, the milk was incubated at 42 °C until the pH decreased to 4.60. At the
end of incubation, yogurt samples were manually stirred and stored at 4 °C. The physicochemical properties of
the stirred yogurt samples were analyzed on day 1 of storage. The results showed that the highest viscosity,
hardness, visual roughness, number of grains and perimeter of grains values were obtained in the stirred yogurt
samples produced from standardized milk using whey protein. However, the lowest viscosity, hardness, visual
roughness, number of grains and perimeter of grains values were determined in the stirred yogurt samples
produced from standardized milk using cream. No significant differences (P>0.05) were found in the total dry
matter, pH and titratable values among the stirred yogurt samples produced using whey protein, inulin and
cream.
Keywords: Whey Protein, Inulin, Cream, Stirred Yogurt, Visual Roughness, Graininess
*
13
COMPARISON OF NATURAL AND CULTURAL FORMS OF VINCA MINOR L.
(APOCYNACEAE) SPECIES GROWING IN TURKEY IN TERMS OF
PHARMACEUTICAL BOTANY
ÜMMEHAN GÜNDÜZ, SEVIM KÜÇÜK
ANADOLU UNIVERSITY
Abstract
Vinca L. is most taxa hosting genus of the family Apocynaceae in Turkey. The natural and cultural forms of
Vinca minor L. species of this genus were evaluated by comparing their morphological and anatomical features.
V. minor leaves are used in antipyretic, appetizing, constipation, diuretic dermatitis, diuretic, coronal disease,
tonsillitis, diarrhea, anemia, hemostatic, bronchitis, gonorrhea, low blood pressure, bleeding arrest, treatment of
tumors, oral ulcers and inflammations, hypertension, heart disease and female gynecological problems.
In morphological studies; stem, leaves and flowers were measured and their characters were examined. While
flower and leaf color were similar, stem length of the specimens in the natural form was measured as 19-65 cm
and culture forms as 10-51 cm. While the number of leaves in natural forms was between 7-20, it was observed
that it was between 7-34 in culture forms.
In anatomical studies; root and stem cross-section, leaf cross-section, upper and lower superficial sections were
examined. As a result of the anatomical studies, while absorbing hairs were observed in the root of the natural
samples, these structures were not found in the culture form samples. In addition, anomocytic stoma was found
on the upper surface of the leaf in the culture form, but not in the natural forms.
Keywords: Apocynaceae, Vinca Minor L. Anatomy, Morphology, Turkey
*This study is supported by Scientific Research Projects Program of Anadolu University
14
NAFION–GRAPHENE COMPOSITE FILM MODIFIED GLASSY CARBON
ELECTRODE FOR VOLTAMMETRIC DETERMINATION OF DOPAMINE
ASIYE ASLIHAN AVAN
ISTANBUL UNIVERSITY-CERRAHPAŞA
Abstract
A Nafion–graphene (Nafion–GR) nanocomposite film modified glassy carbon electrode was fabricated by a
simple drop_casting method, and used in the electrochemical detection of dopamine(DA). Owing to the large
surface area, good conductivity of GR and good affinity of Nafion, the sensor exhibited excellent
electrocatalytic activity for the oxidation of DA. The electrochemical behaviors of DA on Nafion/GR film
modified glassy carbon electrodes were investigated by cyclic voltammetry and differential pulse voltammetry.
A calibration curve is constructed in the same matrix, urine, as the unknown samples to be analyzed. The
Nafion–GR film modified electrode was linearly dependent on the DA concentration and the linear analytical
curve was obtained in the ranges of 0.5–200 μM with differential pulse voltammetry (DPV) and the detection
limit was 0.051 μM. The Nafion_graphene nanocomposite modified electrode exhibited good reusability than
pure graphene-modified GCE. This procedure can be used for the determination of DA in the presence of UA,
AA, and TRP. Finally, the proposed method was successfully used to determine DA in urine samples and
pharmaceutical preparations.
Keywords: Dopamine, Voltammetry, Graphene, Urine Analysis, Pharmaceutical Analysis
*Istanbul University-Cerrahpaşa
15
EVALUATION OF OSTEOPOROSIS KNOWLEDGE MEASUREMENT LEVEL
AND NUTRITION IN WOMEN
AYDAN CANKARA, FATMANUR OZYUREK
ISTANBUL MEDIPOL UNIVERSTY
Abstract
Osteoporosis is the most common disease of bone metabolism, which effects individual independence, physical,
mental and social well-being and results in morbidity, mortality. The aim of this study was to measure the
knowledge levels of osteoporosis in adults with evaluating purpose of nutritional status. The study was planned
descriptive cross-sectional research, consisted of 165 adult female volunteers in Istanbul on February-April
2019. The information of the women was gathered face to face interview method with questionnaire. The
questionnaire includes the Osteoporosis Knowledge Test, 3-day food consumption record. The data of the
dietary records was evaluated with BeBis program. All statistical analyses were analysed with SPSS. The mean
age of women was 38.8 ± 14 years and BMI was normal with 50.9%. Osteoporosis Knowledge Test’s mean
score of all participants was 19.16 ± 4.02. A very weak and significant negative correlation was observed
between age and Osteoporosis Knowledge Test scores. (rho=-0,165*, p=0.034) The daily amount of protein
taken by women is 58.77±62.53 g, vitamin C is 68.67±49.56 mg, calcium is 537.90±212,72 mg and phosphorus
849,04±260,33 mg. It was observed that vitamin C and calcium mineral were lower than recommended in all
age groups of women. Trainings should be planned to increase the knowledge of osteoporosis. Beginning from
young adulthood, people should be given the habit of eating a diet that has a positive effect on bone health and
is protective against osteoporosis.
Keywords: Osteoporosis Knowledge, Women, Nutiriton, Calcium
*
16
THE EFFECT OF EXAM ANXIETY AND BODY IMAGE ON EATING BEHAVIOR
IN ADOLESCENTS
HANDE OTAKLI1, FATMANUR ÖZYÜREK1, NESLİHAN SÜRMELİ2
1MEDİPOL ÜNİVERSİTESİ
2YÜZÜNCÜ YIL ÜNİVERSİTESİ
Abstract
During the adolescent period, healthy nutrition provides the nutrients necessary for the physical, cognitive
growth and development of individuals and reduces the risk of feeding-related diseases at the beginning of
adulthood. Throughout life, despite the change in body perception, especially adolescence is a period in which
body perception and nutrition problems are experienced. In this age group, the desire to reach the ideal body
size determined by the society, changes on the diet and wrong diet practices may cause inadequate and
unbalanced nutrition. This study was conducted in order to examine the effects of exam anxiety and body
perception on feeding behaviors in adolescents. 203 female and 97 male, in total 300 students whose ages from
17 to 19 participated in the study. General information, exam anxiety, body perceptions and eating behaviors of
the participants were measured using a questionnaire prepared in advance. Test Anxiety Scale, Netherlands
Eating Behavior Survey, Body Appreciation Scale have been applied to the adolescents. When exam anxiety of
the students was examined, physical reaction were found to be higher in girls, mental reaction were higher in
boys (p<0,05). It was found that girls received significantly higher scores than boys in restrictive eating and
emotional eating categories when the participants examined their eating behavior (p<0,05). It was found that
obese individuals scored the lowest in restrictive eating behavior and the highest in emotional eating behavior
(p<0,05). It has been observed that there is positive and significant relation between restrictive eating behaviour
and body liking scores (p=0,00, r=0,201). Healthy eating habits are very important for protecting adolescents
from the stress of education, reducing the effects of body perception and coping with stress.
Keywords: Adolescent, Body Perception, Exam Anxiety, Nutrition Behavior
*
International Congress on Applied
Biological SciencesNovember 08-12 I Sarajevo
5TH