3D ModellingIntegrated Life Cycle Sustainability Assessment

Diacids production from 2nd generation bio-refinery

Martina Prox, Michael Bruns, Martin Ramacher, Marten Stock, Mieke Klein,

m.prox@ifu.com

Agenda

Meaning of BioREFINE - 2G

Partners and Work Packages

Life Cycle Sustainability Assessment

Results of LCSA

Conclusion

BioREFINE Project Partners

3Figure: map from https://www.scribblemaps.com/, adapted

Meaning of bioREFINE - 2G

BIOREFINE - 2G approach

Utilisation of otherwise unused C5-wood sugars to produce diacids (throughengineered strains) as starting components for value-added biopolymers

Borregaard – Wood treatment

ULUND & DTU – fermentation strain

Identifiying a strain and a fermentation protocol toenable:

• Up take of C5 sugars

• high production of diacid

Optimizing genetic and regulatory processes

within cells to increase the cells' production of a certain substance

Biotrend - Purification

ecopol tech & AIMPLAS - Polymerization

ifu - Life Cycle Sustainability Assessment

Sustainability:

“Meeting current needs without

compromising the needs of future

generations”

Assessing the impacts of newly

developed process to ensure that

sustainability goals are met.

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Sustainability

Goal of Life Cycle Sustainability Assessment

• fully understand environmental, economic and social impacts of products produced by the newly developed bioprocess

• report on the sustainability of the products to the funding association

• serves as a showcase on the newly integrated methods regarding economic and social indicators and impacts into the framework of an already established Environmental Life Cycle Assessment

• support the dialogue with the project partners on the method of a LCSA and to inform on preliminary results based on the most recent developments

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Setup of Life Cycle Assessment Model

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Figure: ifu (2017)

Sustainability results

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Product Source Feedstock GWP [kg

CO2-eq/kg]

NREU

[MJ/kg]

Land use

[m2/kg]

Bio SA [23] Corn 0.3-1.3 28.0-66.5 1.5-2.6

Bio SA [23] Sugar cane -0.4-2.1 9.1-44.9 1.5-2.6

Bio SA [23] Corn stover -0.2-2.5 15.0-54.5 0.8-1.7

Pchem SA [24] MAn 1.95 59.2

Conv SA [22] MAn 98.2

SA 5 [22] Wood 148

Patent SA [25] Sugar beet 1.96 28.5 1.0

bioREFINE Wood sugars 8.2-12.9 162-211 15.5-28.7

[22] Morales, M., Ataman, M., Badr, S., Linster, S. et al., Sustainability assessment of succinic

acid production technologies from biomass using metabolic engineering. Energy Environ. Sci.

2016, 9, 2794–2805, DOI: 10.1039/C6EE00634E.

[23] European Commission, Environmental Factsheet: Succinic Acid.

[24] Cok, B., Tsiropoulos, I., Roes, A. L., Patel, M. K., Succinic acid production derived from

carbohydrates: An energy and greenhouse gas assessment of a platform chemical toward a bio-

based economy. Biofuels, Bioprod. Bioref. 2014, 8, 16–29, DOI: 10.1002/bbb.1427.

[25] Datta, R., Glassner, D. A., Jain, M. K., Roy, J., Fermentation and purification process for

succinic acid, Google Patents, 1992. http://www.google.com/patents/US5168055.

Social Life Cycle Assessment

• Based on UNEP/SETAC

Guidelines

• Qualitative only

• Questionnaire forsite-specific assessment

• Literature research for specific

region/sector assessment

• Social Hotspot Database to assess supply chain

• Site performance good with very

low to low social risks

• Good performance for background

processes in norway

• Wood and chemicals supply should

be assessed specific when theprocess gets implemented

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Conclusions

• LCSA: integrated modelling should be performed to enableassessments of processes in early design phase

• Preliminary results identified hotspots for ongoing processdesign improvements.

• Uptake of C5-sugars and production of diacid pose double challenge. Additionally complex wood sugar medium includingcontaminants and inhibitorsand requires improvements toenable sustainability goals

• Auxiliaries to condition waste streams need to be reduced. Production of diacid not yet implemented on scale-up. Assessment should be updated with ongoing improvements.

Thank you very much for your attention!

Do you have any questions?

Martina Prox

m.prox@ifu.com biorefine2g.de

ULUND – Strain Engineering

DTU – microbial engineering

Metabolic engineering of strains for production of wide variety of chemicals needs several rounds of genetic interventions

• stress resistance

• robustness

• high fermentation capacity

Biotrend – Purification and Scale Up

ecopol tech – Esterification

AIMPLAS - Polymerization

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WIP – Dissemination and Exploitation

• Marketing material, homepage, cooperations,

and bio-conferences

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Hamburg Institute for Environmental IT

Founded: 1992

Software development

• Software:

• Customizing: Special developments, interfaces

Located: Hamburg

Consulting

• Consulting projects, research projects

• Workshops, Trainings