38166-06(9/27/06) presented at sevpac 2008 – permission granted for use on sevpac website only
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38166-06(9/27/06)
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Arrowhead education center -Okefenokee Joe
black rat snake: Elaphe obsoleta obsoleta
http://www.okefenokeejoe.com/ http://www.youtube.com/watch?v=_U2knmkrKA0
“Okefenokee Joe sings kin to the wind”
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2 masses: The masses first appeared in August of 2006 and grew very rapidly. .
Ventral mandible Dorsolateral to the eye
DX: Abscess, granuloma, lymphoma/neoplasia
Surgical excision Histopathology and culturePresented at SEVPAC 2008 – Permission granted for use on SEVPAC
website only
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Subcutaneous tissue, Granulomas, Multifocal to coalescing, with Intralesional hyphae and arthroconidiating hyphae
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Pantoea agglomorans Enterococcus fecalis Staphylococcus intermedius
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Sabouraud’s Dextrose agar
Mycobiotic agar Incubate at 28-
30oC for four weeks
Examine twice a week for growth
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“ MYCOLOGY MESS”
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Macroscopic Color: wide range Size: wide range Texture: wide range Microscopic- LPCB
Inconsistency in use of terms to describe the microscopic morphology
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Isolate forwarded to The University of Texas Health Science Center at San Antonio for further morphological Characterization
“Chrysosporium anamorph of Nannizziopsis Vriesii”
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independently populating organism with different morphology: Same Biochemical and physiological properties
Anamorph: asexually propagating thallus Teleomorph: Sexuallly propagating thallus Total organism = Holomorph
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Independently propagating anamorphs
Pseudallescheria boydii Graphium eumorphum Scedosporium apiospermum
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No Matches
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Morphologic charecterization Molecular charecterization
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Chrysosporium ophidiicola
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Thanks to VDIL, Bacteriology and Histopathology
section Dr. Deanna Sutton Dr. Melissa Vanmeter Professor Guarro
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Questions?
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