10 5 14 12 7 258 41 23 30   1621 9 1 34 38 324 42 15 35 3 3322 37 28   18 3627 13   29   312   17 40 20 3911 24 26 19   6

No Cultivar Viral Status Symptoms

1 BGD PT nil2 Wan Mun NPT VC, Mott, CF,Rug3 Whagi Besta Minj2  NPT VC ,Mott, CF, Cupping4 BGD NPT VC, Mott,CF,Yellowing5 Sinato-GKA NPT Rug,Cup,L/R6 Wan Mun PT Mott,L/R,CF7 Whagi Besta Minj2  PT Cup,Rug,L/R,CF8 Sinato-GKA PT nil9 Sinato-GKA NPT nil10 BGD NPT VC11 Whagi Besta Minj2  NPT vc12 Wan Mun NPT vc13 BGD PT vc14 Sinato-GKA PT nil15 Wan Mun PT vc16 Whagi Besta Minj2  PT Vc,Cs,L/R17 Whagi Besta Minj2  NPT CF, Mott,VC,Cup,L/R,Rug

No Cultivar Viral status Symptoms18 Wan Mun NPT Mott,CF,Cup19 Wan Mun PT Rug, L/R,Cup20 Sinato-GKA NPT VC,Mott,Cup,L/R,Rug,CF21 BGD NPT VC,Mott,Cup,L/R,Rug,CF22 Whagi Besta Minj2  PT Cup,Rug23 Sinato-GKA PT Cup,Rug,CF,L/R24 BGD PT VC,Mott,Cup,L/R,Rug25 Wan Mun NPT Rug,Mott,Cup,L/R26 BGD NPT Rug,Mott,Cup,L/R,CF,CS27 Whagi Besta Minj2  NPT Rug,Mott,Cup,CF,L/R,CS28 Wan Mun PT Cup29 BGD PT L/R30 Whagi Besta Minj2  PT L/R31 Sinato-GKA NPT Cup,Rug32 Sinato-GKA PT L/R,Cup33 Setosa x Setosa Negative Control VC34 BGD Positve Control nil35 BGD Control outside igloo  

35 Samples Blotted(32 from trial + 3 controls)1. BGD 2. Wan Mun3. Whagi Besta Minj24. Sinato GKA

NCM-ELISA TEST 03/04/13 for 1st Re infestation Trial

Road Map

Mott= Mottling VC=Vein clearingCF=Chlorotic spots

Rug=RugosityCupp=CuppingL/Roll=Leaf Roll 

Road map-03/04/13

FMV

10 5 14 12 7 25

8 41 23 30   16

21 9 1 34 38 32

4 42 15 35 3 33

22 37 28   18 36

27 13   29   31

2   17 40 20 39

11 24 26 19   6

TEST FOR VIRUSES: NCM-ELISA RESULTS

SPFMV infected

Road map-03/04/13CFV10 5 14 12 7 258 41 23 30   1621 9 1 34 38 324 42 15 35 3 3322 37 28   18 3627 13   29   312   17 40 20 3911 24 26 19   6

SPCFV infected

Road map-03/04/13CSV-weak positive10 5 14 12 7 258 41 23 30   1621 9 1 34 38 324 42 15 35 3 3322 37 28   18 3627 13   29   312   17 40 20 3911 24 26 19   6

SPCSV infected

Road map-03/04/13SPVG10 5 14 12 7 258 41 23 30   1621 9 1 34 38 324 42 15 35 3 3322 37 28   18 3627 13   29   312   17 40 20 3911 24 26 19   6

SPVG infected

Cultivar Status FMV MMV LV CFV C-6 MSV CaLV CSV SPVG CMV

BGD NPT Positive               Positive  

BGD PT              Weak Positive Positive  

Sinato GKA NPT                 Positive  

Sinato GKA PT       Positive            

Wan Mun NPT Positive               Positive  

Wan Mun PT       Positive      Weak Positive Positive  

Whagi Besta Minj2 NPT Positive                  

Whagi Besta Minj2 PT Positive               Positive  

BGD Positive control Positive               Positive  

Setosa Negative Control outside 

igloo                    

Setosa x Setosa Negative Control                    

Summary Result of 1st Re infestation