The study on correlation ofspecimen collection terms and

influenza virus detection

E.Erdenejargal1, L.Bayarjargal1, M.Battuvshin1, B.Darmaa2

Virology laboratory, Regional Diagnostic Treatment Center, Orkhon Province1

National Center of Communicable Diseases 2

Background

• The reliable laboratory results are significant role in health care services, epidemiological investigations and other public health activities.

• The laboratory result depends on performance of lab technicians by correct techniques, collection, storage and transportation of appropriate specimens.

What factors affects laboratory test results?

Disease stage

Sensitive and unique

characteristics of reagent

examination

Objective and aims

We put our missions forward to comparatively examining virus detection with sample that is been inspected in Orkhon province virus laboratory in winter season of 2010 to 2011 along with sample type and collecting duration after disease spread.

Materials and methods

In this research work at the Virology laboratory, Regional Diagnostic Treatment Center during November 2010 to March in Orkhon Province.

• Regional Diagnostic Treatment Center, Orkhon Province - 135, • Family hospitals, Orkhon Province - 208, • Central Clinic Hospital , Bulgan province - 85

Total 428: Nasal swab- 338, Throat swab - 90

Materials and methods

RNAs were extracted from clinical samples using extraction kit Bioneer, Korea company

rt RT-PCR were performed using the AccuPower Influenza Real time PCR kit (Bioneer) by Bioneer, EXICYCLER rt PCR system.

Materials and methods

• Samples been delivered in 6 hours from family hospitals and 24 hours from Bulgan province to the laboratory after taken to examination and results been made in 24 to 48 hours.

Result

Influenza Influenza-like illness (ILI)

acute respiratory others not infection (ARI) diagnosis

Relevance between sampling duration and virus detection after disease spread

Samples taken in 1 to 3 days after disease spread

Samples taken in 3 to 7 days after disease spread

Total sample numbers and detected viruses taken from united hospitals and family hospitals

Regional Diagnostic Treatment Center,

Orkhon ProvinceFamily hospitals, Orkhon Province

Central Clinic Hospital ,Bulgan province

Relevance between sample type and virus detection

Nasal swab Throat swab

Sample and detected virus numbers that is collected in last 5 years

Discussion

• There were 25(13.4%) positive for influenza viruses of 186 samples with diagnosed influenza, 17(8.5%) positive for influenza viruses of 200 samples with diagnosed ILI, no any influenza positive for influenza viruses of 22 samples with diagnosed sARI, 2(14.2%) positive for influenza of 14 samples with diagnosed other diseases and 1(14.3%) positive for influenza viruses of 14 samples collected not marked any diagnose in the sample collection form.

• There were detected 25(13.4%) positive of 186 samples with diagnosed as influenza, 17(8.5%) positive of 200 samples with diagnosed as ILI, no any positive of 22 samples with diagnosed as sARI, 2(14.2%) positive of 14 samples with diagnosed other diseases and 1(14.3%) positive of 14 samples collected not marked any diagnose in the sample collection form.

• 40(12.5%) positive from 321 samples collected 1-3 days after onset, 4(5.9%) positive from 68 samples collected 3-7 days after onset, 1 (4.5%) positive from 22 samples collected ≥7 days after onset of disease. The 36(10.6%) samples were positive from 338 nasal swab samples and 9(9.9%) samples were positive from 91 throat swab samples by compared sample type and virus detection rate

Conclusion

• Results of grippe virus detection examination concluded that it is related to sample type and collecting duration factors after disease spread.

Thank you for your attention