25yearsofCryoelectronMicroscopyinSpain:AtributetoJoséL.Carrascosa

Madrid,12-13June2019LectureHall

SchoolofBiologyUniversidadAutónomadeMadrid

PlenarySpeakers

SpeakersN.Abrescia(CIC-bioGUNE)J.Agirre(UniversityofYork)S.Alvira(UniversityofBristol)E.Arias-Palomo(CIB-CSIC)J.M.Carazo(CNB-CSIC)D.Castaño-Díez(Biozentrum)P.Chacón(IQFR-CSIC)M.Coll(IBMB-CSIC)J.Conesa(ALBA)S.Connell(CIC-bioGUNE)J.J.Fernández(CNB-CSIC)R.F.Busnadiego(MPIMarXnsried)C.FernándezTornero(CIB-CSIC)I.Fita(IBMB-CSIC)J.Fontana(UniversityofLeeds)B.Herguedas(LMB)A.  Hierro(CIC-bioGUNE)D.Lietha(CIB-CSIC)Ó.Llorca(CNIO)C.López-Iglesias(MaastrichtUniversity)D.Luque(ISCIII)J.Mar^n-Benito(CNB-CSIC)A.  Mar^nez(MPIMarXnsried)J.Ortega(McGillUniversity)P.PérezNavarro(Biozentrum)V.Rubio(IBV-CSIC)JoséR.Castón(CNB-CSIC)M.Samsó(VirginiaC.University)C.SanMar^n(CNB-CSIC)I.Ubarretxena(Biofisika)I.Usón(IBMB-CSIC)M.Valle(CIC-bioGUNE)J.M.Valpuesta(CNB-CSIC)J.Vargas(McGillUniversity)

WolfgangBaumeisterMPIMarXnsried

JoachimFrankColumbiaUniversityNobelPrizeinChemistry2017

RichardHendersonLMBCambridgeNobelPrizeinChemistry2017

AlasdairStevenNIHBethesda

June12th

9:30–9:55h Openingceremony Chancellor,UniversidadAutónomadeMadrid President,ConsejoSuperiordeInvesSgacionesCienTficas

Session1(Chairman:JoséM.Valpuesta)

09:55–10:45h PlenaryLecture:

JoachimFrank(ColumbiaUniversity.USA) “DissecXonofearlyandlateeventsinbacterialtranslaXonusingXme-resolvedcryo-EM.”

10:45–11:00h NicolaAbrescia(CIC-Biogune.Bilbao)

“AnintegraXveapproachtostudythestructureandcellbiologyofviruses”11:00–11:15h JonAgirre(UniversityofYork.UK)

“ComputaXonalmethodsforcarbohydratestructuredeterminaXon,validaXonandanalysis”11:15–11:45h CoffeeBreak11:45–12:00h SaraAlvira(UniversityofBristol.UK)

"Capturingthebacterialco-translaXonaltranslocaXonmachinery”12:00–12:15h ErnestoArias-Palomo(CIB-CSIC.Madrid)

“MechanisXcinsightsintoDNAreplicaXonusingcryo-EM”12:15–12:30h DanielCastaño-Díez(Biozentrum.Basel.Switzerland)

"AutomatedAlignmentofXltSeriesofCryoElectronTomographydata”

12:30–12:45h PabloChacón(IQFR-CSIC.Madrid) "IntegraXveModelingApproachestoInterpretHigh-ResoluXoncryo-EMReconstrucXons”

12:45–13:00h MiquelColl(IBMB-CSIC.Barcelona)

“Usingcryo-EMandX-raycrystallographytosolveviralportalproteins”13:00–13:15h JavierConesa(ALBAsynchrotron.Barcelona)

“3DcorrelaXvecryosohX-raytomographyandhardX-rayfluorescenceofbreastcancercellstreatedwithan iridiummetallodrug”

13:15–13:30h SeanConnell(CIC-Biogune.Bilbao)

“UsingCryo-EMtoinvesXgatestepsinlatebacterialribosomeassemblyandthepotenXalrolesplayedbythe assemblyfactors”

13:30–14:30 Lunch

25yearsofCryoelectronMicroscopyinSpain:AtributetoJoséL.Carrascosa

Session2(Chairman:JoséL.Carrascosa)

14:30–15:20h PlenaryLecture:

WolfgangBaumeister(MPIforBiochemistry.MarJnsried.Germany) “TheMolecularMachineryofProteinDegradaXon:StructuralStudiesexsituandinsitu”

15:20–15:35h RubénFernández-Busnadiego(MPIforBiochemistry.MarJnsried.Germany) “Decipheringthemoleculararchitectureofmembranecontactsitesbycryo-electrontomography”

15:35–15:50h CarlosFernándezTornero(CIB-CSIC.Madrid)

“StructuralstudiesonRNApolymeraseIunveiluniquemechanismsinribosomalDNAtranscripXon”15:50–16:05h IgnasiFita(IBMB-CSIC.Barcelona)

“FromX-RaystoSubtomogramAveragingandBack:TheAdhesiónComplexofHumanPathogenMycoplasma Genitalium”

16:05–16:20h JuanFontana(UniversityofLeeds.UK)

“ExploringtheroleofendosomalK+asatriggerforbunyavirusentry”16:20–16:35h BeatrizHerguedas(LMB.Cambridge.UK)

“StructuralinsightsintoAMPAreceptorcomplexes”16:35–17:05h CoffeeBreak17:05–17:20h AitorHierro(CIC-Biogune.Bilbao)

“Endosomalretrievalofcargoviaproteinscaffolds”17:20–17:35h DanielLietha(CIB-CSIC.Madrid)

“Structuralbasisofmembraneassociatedadhesionsignalling"17:35–17:50h ÓscarLlorca(CNIO.Madrid)

”StructureandremodelingoftheAAA-ATPaseRUVBL1-RUVBL2”17:50–18:05h CarmenLópezIglesias(MaastrichtUniversity.Netherlands)

“Insitucryo-EM:ourachievementswithbacterialsecreXonsystems"18:05–18:20h DanielLuque(ISCIII.Madrid)

“Rotavirusentrymachinerystructure”18:20–18:35h MontserratSamsó(VirginiaCommonwealthUniversity.USA)

“UnveilingthesecretsofanionchannelusingcryoEM”

25yearsofCryoelectronMicroscopyinSpain:AtributetoJoséL.Carrascosa

June13th

Session3(Chairman:JoséR.Castón)09:30–10:20h PlenaryLecture:

RichardHenderson(LMB.Cambridge.UK) "TrendsandchallengesincryoEM”

10:20-10:35h AntonioMarTnez(MPIforBiochemistry.MarJnsried.Germany)

“Template-freedetecXonandclassificaXonofheterogeneousmembrane-boundcomplexesincryo-electron tomograms”

10:35–10:50h JoaquínOrtega(McGillUniversity.Toronto.Canada)

“FromcluelesstoCanada.Storyofanunlikelyjourney”10:50–11:05h PaulaPérezNavarro(Biozentrum.Basel.Switzerland)

“CharacterisingParkinson'sdiseasehumanbrainXssueby3Delectronmicroscopy”

11:05–11:35h CoffeeBreak11:35–11:50h VicenteRubio(IBV-CSIC.Valencia)

”UsingcryoEMtoshedlightonamonogenicdisorderthathastwopresentaXonsandtwomodesofinheritance”11:50–12:05h IbanUbarretxena(Biofisika.UPV/CSIC.Bilbao)

“Cryo-EMstructureofaDNApolymeraseinvolvedineukaryoXcgenomestability12:05–12:20h IsabelUsón(IBMB-CSIC.Barcelona)

“PhasingmicroEDdatawithcryoEMmapsinARCIMBOLDO”12:20–12:35h MikelValle(CIC-Biogune.Bilbao)

”ExploringplantfilamentousvirusesbycryoEM”12:35–12:50h JavierVargas(McGillUniversity.Toronto.Canada)

”ComputaXonalstrategiestoprocesshighlyheterogeneouscryoEMsamples”12:50–13:05h JoséJ.Fernández(CNB-CSIC.Madrid)

”ConsideraXonofthesamplemoXonincryoET”13:05–13:20h JaimeMarTn-Benito(CNB-CSIC.Madrid)

“InfluenzaAtranscripXonandreplicaXonmechanismsrevealedbycryoEM”13:20–14:30h Lunch

25yearsofCryoelectronMicroscopyinSpain:AtributetoJoséL.Carrascosa

Session4(Chairman:JoséM.Carazo)

14:30–15:20h PlenaryLecture: AlasdairSteven(NIH.Bethesda.USA) “PhaseTransiXonsinCryo-EMandVirusAssembly”

15:20–15:35h CarmenSanMarTn(CNB-CSIC.Madrid)

“AcryoEMlookatcomplexvirusassembly:thecaseofhumanandnon-humanadenoviruses”15:35–15:50h JoséRCastón(CNB-CSIC.Madrid)

“CryoEMatnearatomicresoluXonofviruses:ahistoricalperspecXve”

ThetributetoPepewillincludesomewordsby:RebecaBocanegra(IMDEANanociencia),AnaCuervo(CNB-CSIC),LuisEnriqueDonate(WoodGreenResearchSupportServices.London),BorjaIbarra(IMDEANanociencia),MaríaIbarra(CNIO),MarianoEsteban(CNB-CSIC),SergioMarco(Sanofi.France),EvaNogales(UCBerkeley.USA),JuanOrTn(CNB-CSIC),CrisSnaRisco(CNB-CSIC),MargaritaSalas(CBM-CSIC)

JoséM.Valpuesta(CNB-CSIC.Madrid) “25yearsofstudiesonmolecularchaperones”

JoséM.Carazo(CNB-CSIC.Madrid) "MightItalktoDr.JoseLuisCarrascosa.ItisaboutadvicingaPhDThesis”

JoséL.Carrascosa(CNB-CSIC.Madrid)

“Adayinthelife”

25yearsofCryoelectronMicroscopyinSpain:AtributetoJoséL.Carrascosa