22160'02pd rfm300+ user guide en - daihan...

$: 22160'02PD RFM300+ User Guide EN - DAIHAN Sciimg.daihan-sci.com/allforlab/pdf/Refractometer_RFM300... · 2014-01-07 · Instrument description 22-160’02 Rev. D July 2009 Section$
RFM300+ Refractometer

User Guide

RFM300+ User Guide (Eng) Code 22-160 Issue 02 Revision D Date November 2008 Bellingham and Stanley Ltd. has been manufacturing high quality optical instruments in the UK since 1914 and is a leading manufacturer of refractometers and polarimeters. The current range of products includes optical and digital hand refractometers as well as a full range of laboratory refractometers and polarimeters available through a network of trained distributors throughout the world. Process refractometers are also available through specialist outlets. Our main website gives full details about Bellingham and Stanley Ltd. and our products. Foreign language brochures in PDF format may be downloaded from this section of the site by clicking the flag. We apologise if the foreign language brochure of your choice is not available. Contact Sales at Bellingham and Stanley Ltd. to discuss a particular application or to receive details of your local distributor. Website: www.bellinghamandstanley.com Bellingham + Stanley Ltd. recognise all trademarks © Copyright Bellingham + Stanley Ltd. 2009 Every effort has been made to ensure the accuracy of the contents of the manual. However, Bellingham + Stanley Ltd. can assume no responsibility for errors contained in the manual or their consequences. Printed in UK

Bellingham + Stanley Ltd. Bellingham + Stanley Inc. Longfield Road, 1000 Hurricane Shoals Road, Tunbridge Wells, Kent TN2 3EY Building D, Suite 300, United Kingdom Lawrenceville, USA GA30043 Tel: +44 (0) 1892 500400 Tel: 770 822 6898 Fax: +44 (0) 1892 543115 Fax: 770 822 9165

DECLARATION OF CONFORMITY According to ISO/IEC 17050-1 & 2 : 2004

Manufacturer's Name Bellingham & Stanley Limited Manufacturer's Address Longfield Road, Tunbridge Wells, Kent TN2 3EY United Kingdom

declares that the product Product Name RFM300+ Refractometer Model Number All Is designed to conform to the following Product Specifications: Safety BS EN 60950-1:2002 EMC Emissions

• BS EN 55022:2006 Radiated Emissions Class A • BS EN 55022:2006 Conducted Emissions Class A

Immunity

• BS EN 61000-3-2:2006 Harmonics • BS EN 61000-3-11:2001 Flicker • BS EN 61000-4-2:1995 ESD 8kV contact • BS EN 61000-4-3:2006 EMS 3V/m • BS EN 61000-4-5:2006 Surges 0.5kV (line to line)

1.0kV (line to earth) • BS EN 61000-4-11:2004 Power outages 1 cycle/100%

Supplementary The product herewith is designed to comply with the requirements of the EMC Directive 2004/108/EC and the Low Voltage Directive 2006/95/EC.

This symbol is an internationally agreed indicator that the product bearing it should not be disposed of as general waste or garbage which might end up in landfill sites, but should instead be sent for special processing and/or recycling in those countries where appropriate legislation and facilities are in place.

This symbol indicates a caution or warning, please refer to the manual.

Contents 22-160’02 Rev. D July 2009

contents

Installing the instrument ............................................. 1 Instrument description................................................ 2

Instrument overview ............................................ 2-1 Basic operation ................................................... 2-4 Display screen information .................................. 2-9 The instrument logs............................................. 2-14 Measurement settings......................................... 2-15 Measurement settings in detail............................ 2-16 Remote operation................................................ 2-18 Sampling techniques........................................... 2-19 Use of temperature compensation ...................... 2-20 Air filter replacement ........................................... 2-21

Setting up the system ................................................ 3 Menu flow chart................................................... 3-1 Shortcut keys ...................................................... 3-3 Calibration ........................................................... 3-5 Measurement settings......................................... 3-8 Methods .............................................................. 3-9 User accessibility ................................................ 3-15 System settings................................................... 3-16 Customising the instrument................................. 3-20 Measurement traceability .................................... 3-22 Adding extra scales............................................. 3-24 Application Corrections ....................................... 3-26 Setting the default values.................................... 3-28 Displaying the software / hardware details .......... 3-28

Specification............................................................... 4 Spares and accessories............................................. 5

Installing the instrument 22-160’02 Rev. D July 2009 Section 1

section 1

Installing the instrument Unpacking the instrument.................................... 1-1 Contents list ........................................................ 1-1 Part numbers....................................................... 1-1 Positioning the system ........................................ 1-2 Mains connection ................................................ 1-2 Power requirements ............................................ 1-2 Power supply adapter ......................................... 1-2

Installing the instrument 22-160’02 Rev. D July 2009 Page 1-1

Unpacking the instrument Carefully remove all of the packing material. It is recommended that the box and other packing materials are retained so that, should the need arise, the instrument can be safely returned to the manufacturer. Check that all parts listed below are present and that no transit damage has occurred. If any are damaged or missing, contact the supplier immediately.

Contents list 1 RFM300+ Refractometer module, see table below for part number 1 Power supply, 55-105 1 Mains lead, see below for part number 1 Operating Instructions, 22-160 1 Instruction manual CD-ROM, 55-300 1 Interconnecting lead (PC serial), 54-07 4 Spare Air Vent filter

Part numbers RFM300+ refractometer part numbers

Model Complete refractometer including power supply

Refractometer module only

RFM330+ 22-30 22-330

RFM340+ 22-40 22-340

Mains lead part numbers (for use with Power supply 55-105)

Mains cord wire colours

Moulded plug type for Voltage Line

(Phase) Neutral (Return)

Earth (Ground)

Code no.

Switzerland 230V - - - 61-181

Denmark 230V - - - 61-182

India / South Africa 230V - - - 61-188

Australia 230V - - - 61-189

No plug – open lead - Brown Blue Green / Yellow

61-190

UK 13 Amp square pin to BS1363/A

230V - - - 61-191

United States (3 pin) 110V - - - 61-192

Europe (Schuko) 230V - - - 61-193

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Page 1-2 22-160’02 Rev. D July 2009 Installing the instrument

Positioning the system Place the instrument on a flat and stable bench that is: • dry and indoors • away from draughty or hot equipment like fans or heaters • out of direct sunlight or strong ambient light • away from potential sources of interference, such as RFI generating

equipment • within reach of a power point • not using a power circuit that also has large motors or noise generating

equipment connected to it

Mains connection The power supply adapter is supplied with a moulded mains cord and plug, to suit one of several socket types. For UK lead, replace fuse only with the type indicated on the plug.

Power requirements Voltage 110 to 230 V~ ±10% Frequency 50 to 60 Hz Maximum current 2 A

Power supply adapter 55-105 RISK OF ELECTRIC SHOCK: • For electrical safety information, read the label on the power supply • For indoor use only • Must be kept dry • Disconnect the equipment from the mains supply before unplugging the

mains lead from the power supply unit • Do not open the power supply adapter - no user serviceable parts inside WARNING: • Do not cover, designed to operate with free air convection • No cleaning required Note: A waterproof power supply adaptor, code no. 55-250, which can be

used in damp environments, is available as an optional extra

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Instrument description 22-160’02 Rev. D July 2009 Section 2

section 2

Instrument description

Instrument overview................................................... 2-1 The refractometer................................................ 2-1 The services panel .............................................. 2-2 The display panel ................................................ 2-3

Basic operation .......................................................... 2-4 Switching on........................................................ 2-4 Switching off........................................................ 2-4 Switching on for the first time .............................. 2-4 Initial operation after switching on ....................... 2-5 Manoeuvring through the menus......................... 2-6 Keying in letters, numbers and other characters . 2-7 Keying in numerical values.................................. 2-8

Display screen information......................................... 2-9 Screen title .......................................................... 2-9 Current time ........................................................ 2-9 Softkey functions................................................. 2-9 Temperature control status ................................. 2-10 Taking a reading ................................................. 2-11 Measurement display description........................ 2-12 Reading quality ................................................... 2-12 Printing the readings ........................................... 2-13 24 column print format ........................................ 2-13 Printer options ..................................................... 2-13

The instrument logs ................................................... 2-14 The System log ................................................... 2-14 The Reading log.................................................. 2-14 PC Data Acquisition Program.............................. 2-14

Measurement settings................................................ 2-15 Mode menu ......................................................... 2-15

Measurement settings in detail .................................. 2-16 Scale ................................................................... 2-16 Temp. Comp. (temperature compensation)......... 2-16 Setting the temperature control setpoint ............. 2-17 Stability................................................................ 2-17 Limits................................................................... 2-17

Remote operation ...................................................... 2-18 RS232 serial port ................................................ 2-18

Sampling techniques.................................................. 2-19 Cleaning.............................................................. 2-19 Sample application.............................................. 2-19 Sample presser ................................................... 2-19 Temperature stability........................................... 2-19

Use of temperature compensation ............................. 2-20 ‘Sugar’ temperature compensation ..................... 2-20 ‘AG Fluid’ temperature compensation ................. 2-20 Alternative temperature compensations.............. 2-20

Air filter replacement .................................................. 2-21

Instrument description 22-160’02 Rev. D July 2009 Page 2-1

Instrument overview

The refractometer

Presser:Minimises heat loss/gain. Excludes strong ambient light from prism. Minimises sample evaporation. An alternative presser insert is available depending on application (page 5-2 and 5-4).

Drip dish: PEEK plastic — excellent chemical resistance and provides thermal isolation.

Prism plate: 316 Stainless steel with a silicone rubber and resin prism seal providing excellent chemical resistance.

This surface can become extremely hot.

Measuring prism: Artificial sapphire.

Cooling air inlet vent: Air passes through ducting within the instrument and is expelled through vents at the rear. This ensures the inside of the instrument is sealed from the environment.

These vents must not be covered.

Housing:Low density expanded polyurethane foam. Lightweight yet good mechanical strength.

Display panel: see page 2-3.

Page 2-2 22-160’02 Rev. D July 2009 Instrument description

The services panel

CE mark: Specifies the instrument conforms to relevant EU safety and EMI regulations.

Code number: B&S code number for the instrument module.

Cooling air outlet vents:Air is drawn in through the side vents and passes through ducting within the instrument before being expelled through these vents. This ensures the inside of the instrument is sealed from the environment.

These vents must not be covered.

Parallel port: For printing.

Serial number:Always specify this number in any communication with B&S Ltd.

Warning symbol: See similarly marked warnings in this manual.

RS232 Serial port 1:For remote communication or printing.

Power connector: 24V dc from power supply.

RS232 Serial port 2:For connection to barcode reader.

Disposal symbol: See ‘Declaration of Conformity’ page in this manual


The display panel

Power indicator STANDBY — Red. ON — Green.

Temperature Control status indicators:

Arrow keys Forward/Back keys for negotiating through menus etc..

Power key: Press to switch ON. Press for 2 seconds to switch to STANDBY.

Soft key functions

Alpha-numeric keys‘Telephone style’ keys for entering letters and numbers

Soft keys Functions change with display.

Operation status bar Shows the details of the current operation.

Current time


Basic operation

Switching on Plug in the power supply and switch on the mains supply. The instrument will display configuration data and then switch to standby mode with the power indicator showing red. To switch on, press the Power Key – the Power Indicator will show green, and the following will be displayed.

Switching off

To switch to standby, press the Power Key and hold down until a confirmation screen shows (approximately 2 seconds). Press Yes to switch to standby – the Power Indicator will show red, or press No to continue operating. Note: It is preferable for the instrument to be left on continuously even if a reading is to be taken only once per day.

Switching on for the first time When the instrument is switched on for the first time after delivery, a ‘Setup Wizard’ will step you through a number of questions so that the system can be configured for your particular application. Initially, it is a requirement to select the language. Step 1 Setup the standard measurement conditions. These are the

settings which measurements are to be taken, the reading scale and temperature compensation that should be used. When the correct conditions are selected, press Quit.

Step 2 Set the clock. Adjust the instrument clock to local time. Step 3 Set the printer options. Select whether readings are to be

printed, stored in memory or both. When the correct conditions are selected, press Quit.

The Setup Wizard can be run at any time by selecting the Tools menu.

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Initial operation after switching on When the instrument is switched on, the temperature control system will be turned on and the fans will blow air out through the Cooling Air Outlet Vents. The control system aim temperature will initially be set to 20ºC. The screen shown below will be displayed.

Depending upon the ambient temperature it might take up to 10 minutes to reach 20°C.

Once the temperature has stabilised, a message will be displayed informing the user that an Auto Calibration is required, and to login.

Press OK and the instrument will request that the supervisor PIN is entered. By default the supervisor PIN is 135, see page 3-15 for more information about PINs. Logging in is only necessary if PINs are active.

With the PIN entered, the instrument will request that the prism is cleaned and that the presser is lowered.

It is vital that the complete prism face is scrupulously clean and dry. Press OK.

A Zero point calibration must then be carried out. The Zero sample value must be entered. This will initially be 0.00º Brix (distilled water). Press Enter. Place a small amount of distilled water onto the Measuring Prism. The

complete prism surface must be covered by the water; generally 1ml is sufficient to achieve this.

Lower the presser. Press OK. There will then be a delay while the temperature is stabilising. When the calibration has been set, the Measurement Display will be

shown. The refractometer is now ready for use. It is recommended that the Zero calibration should be repeated daily, see page 3-5.

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Manoeuvring through the menus Menu items can be found and selected by either: pressing the Up/Down softkeys and then pressing Select or pressing the item number key i.e. press 3 for Setup

If the menu item contains a list of options to choose from then either: select the menu item and then choose the option from the displayed list or press the Forward/Back arrow keys to step through the options

Menu items that can be modified by using the arrow keys are identified by arrows being displayed next to the menu items value (highlighted below).

Return to the Measurement display by repeatedly pressing Quit to step back through the menus. BBaa ss

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Keying in letters, numbers and other characters When it is necessary to enter in text, such as a method name, the alpha-numeric entry screen will be displayed. This will allow entry of numbers, letters and a range of punctuation characters including “ ! ? / ( ; etc. by multiple presses of keys.

E.g. To enter the name cola(74) press: the key 3 times c the key 3 times o the key 3 times l the key 1 time a the key 11 times ( the key 5 times 7 the key 4 times 4 the key 12 times ) Once a key is pressed, the available characters that can be selected using that key are shown at the top right of the screen (see the table below). If a key is not re-pressed within one second, the displayed character will be accepted. Key Available characters

1 - ? ! , . : ; “ ‘ ( ) 1

2 a b c 2

3 d e f 3

4 g h i 4

5 j k l 5

6 m n o 6

7 p q r s 7

8 t u v 8

9 w x y z 9

0 + $ % / # < = > _ 0

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Keying in numerical values Numerical values, such as a refractive index 1.33299 or a polynomial constant 0.0123456, can entered in either fixed decimal point format or in scientific notation. E.g.:

Fixed decimal point Scientific 1.2345 1.2345E0

-123.45 -1.2345E2 0.00012345

or

1.2345E-4 One of the softkeys (second from left) gives access to the minus (-), decimal point (.) and exponent (E) symbols that will be required to enter both numerical formats. As the user enters the number, the instrument responds to the key strokes by changing the soft-key function.

If an error is made while entering the number, press the CLEAR softkey to erase the entry. The ENTER soft-key is available throughout the process and may be pressed at any time to accept the entry. Note that if the number being entered is negative, then press the minus soft-key before entering the number. Otherwise, if the number is less than 1, press 0 first to show the decimal point soft-key.

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Display screen information

The pictures below describe standard user interface elements.

Screen title

The screen title will describe what is being displayed on the screen.

Current time

The time is displayed in the 24 hour clock. To adjust the time / date see page 3-16.

Softkey functions

The softkey functions change depending upon what inputs are required at that time. If a softkey appears grey then that button is disabled. BBaa ss

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Temperature control status

Thermometer symbol —indicates temperature control system is enabled

Aim temperature as defined in the measurement settings

The temperature of the sample

r The temperature control system has yet to stabilise for the

first time after either switching on or a change to the aim temperature.

a The temperature control loop is stable.

↑ The temperature control system is making an increase correction.

↓ The temperature control system is making a decrease correction.

≀

The temperature control loop is stablising.

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Taking a reading Raise the presser to access the prism. The screen will display a message asking for the presser to be lowered. With the presser raised, the temperature control is limited, therefore it is beneficial to limit the time the presser is raised.

Clean the prism thoroughly using a suitable solvent, e.g. water or methyl alcohol depending on the sample type being measured. Place a small amount of water or sample on the prism (the circular glass-like area in the middle of the prism plate). The complete prism surface must be covered by the sample; generally 1ml is sufficient to achieve this. Lower the presser, the message to lower the presser will clear. Press the Read softkey. The instrument will display a progress bar whilst data is collected and the measurement calculated.

The reading will be displayed using the scale and temperature compensation set in the ‘Mode’ menu as described on page 2-16.

The following page describes what each parameter on the measurement display is.

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Measurement display description

The measurement value is shown in large figures for easy readability. It is calculated using the measurement settings shown below.

The measurement settings include the measurement scale and temperature compensation used (see page 2-15).

The temperature measured when the reading was taken is displayed in degrees Celsius.

The quality measured when the reading was taken. See below for details on the quality value.

Reading quality Quality is an arbitrary figure used to describe the worth of a reading. The quality value is derived from the optical pattern caused by placing a sample on the prism. A high quality value indicates a well defined optical pattern which in turn makes the signal easier to resolve; a low value indicates a less well defined pattern and hence a less reliable reading. The quality value for the sample used to set ZERO (normally distilled water) is automatically set to 100, which can then be used as a reference with which to compare other measured samples.

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Printing the readings If the fourth (right hand) softkey is set to ‘Print’ or ‘Prt+Save’, the last displayed reading can be sent to a printer via the selected port. The print layout can be either with the reading data listed sequentially, suitable for narrow printers with 24 columns, or with the data in CSV (comma separated values) suitable for connection to a LIMS. (See ‘Set the print mode’ and ‘Available print formats’ in Section 3)

24 column print format

At the start of the printout is a header. This contains general information about the instrument, such as when the last span was carried out, and measurement settings. Each measurement has a number of parameters. On the left is the parameter name, and on the right is the parameters value. Use interconnecting cable 54-03 to connect the instrument to a parallel printer. (These cables are available from Bellingham + Stanley. Some devices may require alternatives.)

Printer options Optionally the reading can be sent to the RS232 serial port for connection to either a printer or computer terminal program.

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The instrument logs This instrument has two separate logs for storing data. This data will be held in the instrument whether the power is on or off and can be downloaded to a computer using the PC Data Acquisition Program (see below).

The System log The System log maintains a record of all changes and adjustments made to the instrument by the operators. This will include each zero and span calibration, changes to the setup, configuration and methods.

The Reading log If the fourth (right hand) softkey is set to ‘Save’ or ‘Prt+Save’ then pressing it will save the last displayed reading into the Reading log. Up to 712 readings can be stored in the log. When the log is full no further results will be able to be stored, a warning message will be displayed on the screen. The current status of the Reading log can be viewed by selecting the ‘Saved results’ menu option with in the Tools Menu. The reading log and current status can be viewed by selecting the ‘Saved results’ menu option within the Tools Menu. Press the ‘Review’ softkey to display the contents of the log. When displayed, pressing the arrow keys will show additional reading parameters.

PC Data Acquisition Program

The PC Data Acquisition Program (B+S code no. 26-303) enables the data from both of the above logs to be transferred to a PC at which time the logs will be deleted in the instrument. The data can then be sorted, easily viewed and also saved in csv format files, which can then be opened in a spreadsheet. This program can be downloaded free from the Bellingham + Stanley website…

www.bellinghamandstanley.com

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Measurement Settings The measurement settings are accessed in the Mode menu, which is available from the Measurement Display.

Mode menu The mode menu will be familiar to users of other Bellingham + Stanley instruments. The measurement settings are changed from the Mode menu, which is accessed from the Measurement Display.

Press Quit to return back to the Measurement Display. The following pages describe each of the measurement settings.

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Measurement settings in detail This instrument has a number of measurement settings… Scale (the scale units that the readings are displayed in) Temp. Comp. (whether temperature compensation is used) Set Temp. (temperature the instrument will control the prism to) Stability (can be set so that a measurement will only be taken if the

sample has stabilised) Limits (tests to check if the reading parameters are within set

limits)

Scale The instrument has a number of scales that measurement can be displayed in. By default the available scales are…

Scale Name Scale ID Refractive Index ri

Brix bx Additional scales can be added, see page 3-24 for details.

Temp. Comp. (temperature compensation) The instrument has a number of temperature compensation modes available. By default the available temperature compensation modes are…

TC Name TC ID none no sugar su

ag fluid ag Additional temperature compensation modes can be added, see page 3-25 for details.

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Setting the temperature control setpoint The temperature control system in the instrument can be set over a wide range of temperatures. For most applications, it is likely that all samples will be measured at the same temperature; e.g. 20°C for a bottling plant or 70°C for a preserves factory. However, some major food manufacturers who produce a range of different products at one facility may need to measure different ranges of samples at more than one temperature, e.g. soft drinks at 20°C and a range of edible oil products at 60°C. To cater for this requirement, the temperature control setpoint is available as one of the method parameters. However, this must be used with care. It is not practical to continually adjust the temperature setpoint for every sample. After a change to the setpoint, the instrument will inhibit reading display until the system has stabilised and a ‘zero’ calibration routine has been carried out. Changing the setpoint will only be practical if, say, all samples to be measured at 20°C were carried out in a morning session and then those at 60°C in the afternoon. The temperature of the stainless steel prism plate will be similar to the temperature control setpoint and so could become extremely hot. Direct contact with skin should be avoided when applying sample or cleaning the prism plate at temperatures above 50°C.

Stability The instrument can automatically detect if the sample has stabilised. There are number of stability modes from a simple time delay (wait 10 seconds before taking the measurement) to more sophisticated modes. This feature is explained in detail from page 3-13.

Limits The instrument is able to have limits set on a number of parameters. If the measured sample fails to meet these limits a line will be drawn through the result.

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Remote operation Operation of the instrument can be controlled remotely from a computer. The 54-07 interconnecting cable that is supplied will connect to the COM port of a typical PC and the instrument’s RS232 Serial Port 1. Use a proprietary terminal program or custom software to send commands and receive data. The instrument default RS232 configuration is:

9600 Baud, 8 bit, no parity Contact Bellingham + Stanley Ltd for a list of serial commands. When the instrument receives a serial command, the display will show: When the action has been completed and the data sent, the instrument will revert to the normal reading display.

RS232 serial port The RS232 serial ports can be accessed via the 9 pin “D type” connectors on the rear of the instrument. The interconnecting cable socket can be secured with the 2 screws but these must be finger-tightened only – DO NOT OVER-TIGHTEN.. 9 Pin “D type” plug connections

Pin No. Function Data direction 2 Received data in 3 Transmitted data out 4 DTR In 5 Signal Ground 6 DSR Out

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Sampling techniques In order to achieve the maximum performance from the refractometer, it is essential that extreme care is taken when cleaning the instrument and applying sample to the prism. Sample concentration may vary considerably from the surface to the centre of a mass whether in a beaker or on a spoon or spatula. Evaporation can cause remarkably rapid drifting unless care is taken.

Cleaning Always clean both the prism surface (and the presser if it contacts the sample) immediately after taking readings. Sample that has remained on the prism for long periods can be difficult to remove and small particles of dried matter can subsequently dissolve into later samples. Use clean tissue with water to remove old sample then dry with fresh clean tissue. Some samples, particularly oils and other chemicals, will require the use of a solvent, such as methyl alcohol, for removal and cleaning. NEVER use acetone, white spirit, 'Genklene', 'Arklone' or any abrasive cleansers on any painted surface or, particularly, the membrane keypad panel.

Sample application Always take sample from the centre of a container not from the surface where higher concentration skin layers will be present. Keep the time taken to transport the sample from the container to the prism to an absolute minimum to limit evaporation. With low viscosity liquids, use a disposable pipette once only. With high viscosity samples, scoop out sufficient with a clean, dry spatula. Never pour a sample directly from a container onto the prism. The temperature of the stainless steel prism plate could become extremely hot. Direct contact with skin should be avoided when applying sample or cleaning the prism plate at temperatures above 50°C.

Sample presser

The sample presser will improve measurement accuracy and repeatability by:- 1. Providing an 'enclosed' environment for the sample so minimising

contact with the air and limiting evaporation. 2. Minimising the effect of ambient air temperature on the controlled

sample. 3. Preventing strong ambient light from affecting the reading quality.

Temperature stability The refractive index of liquids changes significantly with temperature. It is important that the temperature of a sample is constant throughout its mass before an accurate reading can be taken.

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Use of temperature compensation Selecting the temperature compensation mode corrects readings from the refractometer to a reference temperature, e.g. 20°C. That is, it allows the refractometer to run at the temperature of the product under test but produces readings as if the system was running at the reference temperature. However, temperature compensation should not be considered as just an alternative to temperature control. If the sample temperature is changing (e.g. after a warm sample is applied to a cool prism) then there is inevitably a delay between the actual temperature of the sample in contact with the prism face and the temperature detected by the temperature probe; (the probe cannot have instant response). Therefore, there is bound to be some degree of inaccuracy from the compensation calculations. Temperature compensation will only be valid if the detected sample temperature is stable, so there is a great advantage in controlling the prism temperature to rapidly achieve stability. Using temperature compensation together with the temperature control system in the instrument could be an advantage in certain applications. Typically, a hot sample could be taken from a production line and placed on an instrument controlled at 60°C but with compensation to display the equivalent reading at 20°C.

‘Sugar’ temperature compensation ‘sugar’ temperature compensation will correct readings of water and sucrose solutions to 20°C. It conforms to the published ICUMSA 1978 correction tables which covers the ranges 10 to 40°C and 0 to 80° Brix and has been extended to cover 5 to 70°C by using additional data. Although the correction is specifically applicable to pure sucrose solutions, it is also valid for many sugar based food products. However, it must be stressed that the correction values may be unsuitable for other, non-sugar based, products and great care should be exercised if ‘sugar’ temperature compensation is used with these samples.

‘AG Fluid’ temperature compensation ‘ag fluid’ temperature compensation will correct readings of AG Fluids to 20°C, it covers the range 10 to 40°C. AG Fluids are a part of a range of Calibration Materials available from Bellingham + Stanley which can be used for verifying and setting the instrument calibration. The reading variation with temperature of AG Fluids is different from that of sugar and so ‘ag fluid’ temperature compensation is useful when calibrating the instrument at temperatures other than 20°C.

Alternative temperature compensations Alternative temperature compensations can be added for the correction of sugar solutions to reference temperatures other than 20°C or for other product types. For instructions on how to add custom temperature compensation modes see page 3-25.

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Air filter replacement This instrument is fitted with replaceable filters on the cooling air inlet vents. The filters will prevent airborne dust or grease entering the ducting and clogging the fans. They should be removed and replaced at regular intervals. The replacement interval could be weekly for environments with high levels of atmospheric contaminants or up to three monthly for clean room applications. Initially, check the filters often to determine how quickly they become choked.

To replace the filter, pull the filter cover away from the instrument. Four spare filters are supplied with the instrument. Additional filters are available from Bellingham+Stanley in packs of 20 (B+S code no. 26-292).

To replace an enhanced protection filter, unscrew the filter holder from the instrument body by turning it in an anti clockwise direction. Pulling the filter holders slightly away from the body can aid their removal. Once the filter is replaced, simply screw back on. Four spare filters are supplied with the instrument. Additional filters are available from Bellingham+Stanley in packs of 20 (B+S code no. 22-088).

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Section 3 22-160’02 Rev. D July 2009 Setting up the system

section 3

Setting up the system

Menu flow chart.......................................................... 3-1 Shortcut keys ............................................................. 3-3 Calibration.................................................................. 3-5

Calibration standard values................................. 3-5 Zero calibration ................................................... 3-5 Span calibration .................................................. 3-6 Cancel the last calibration ................................... 3-7 Calibration report................................................. 3-7

Measurement settings................................................ 3-8 Which to use, the Mode menu or Methods?........ 3-8 To enable the use of Methods............................. 3-8 To disable the use of Methods (if using the Mode menu) ................................................................. 3-8

Methods ..................................................................... 3-9 Adding a new method ......................................... 3-9 Editing an existing method .................................. 3-10 Hiding the preset methods .................................. 3-10 Method selection type ......................................... 3-11 Print a list of methods.......................................... 3-11 Reading limits...................................................... 3-12 Adding a limit test condition................................. 3-12 Editing or deleting a limit test condition ............... 3-12 Reading stability.................................................. 3-13 Adding a stability delay condition ........................ 3-13 Adding a stability repeatability condition ............. 3-14 Using smart stability detection............................. 3-14

User accessibility ....................................................... 3-15 Entering PINs ...................................................... 3-15 Changing PINs .................................................... 3-15

System settings.......................................................... 3-16 Setting the date format........................................ 3-16 Adjust clock for summer (daylight saving) time ... 3-16 Set the clock time................................................ 3-16 Set the print mode............................................... 3-17 Available print formats......................................... 3-17 Select use of ports (serial 1 and parallel) ............ 3-17 Using statistic print mode .................................... 3-18 Automatic printing/saving .................................... 3-19 Set the serial port configuration........................... 3-19

Customising the instrument........................................ 3-20 Change the display layout ................................... 3-20 Change the reading recurrence........................... 3-20 Change the reading resolution ............................ 3-20 Adjust the display backlight and polarity ............. 3-21 Disabling the presser .......................................... 3-21

Section 3 22-160’02 Rev. D July 2009 Setting up the system

Measurement traceability ........................................... 3-22 Batch codes ........................................................ 3-22 Using barcodes as batch codes .......................... 3-23 User codes .......................................................... 3-23

Adding extra scales.................................................... 3-24 Adding a standard scale...................................... 3-24 Adding a custom designed scale......................... 3-24 Adding a custom designed temperature compensation...................................................... 3-25

Application Corrections .............................................. 3-26 Offset Correction ................................................. 3-26 Citric Acid Correction .......................................... 3-27

Setting the default values........................................... 3-28 Displaying the software / hardware details................. 3-28

Setting up the system 22-160’02 Rev. D July 2009 Section 3-1

Menu flow chart

Menu 1. Saved Results

2. Setup Wizard…

1. Methods 2. Passwords 3. Display 4. Reading 5. System

1. Zero 2. Span (Top

Calibration) 3. Undo 4. Report

1. Calibration 2. Tools 3. Setup 4. Information 5. Help

1. Language 2. Time / Date 3. Communications 4. Traceability 5. Temp. Control

1. Use Methods 2. Hide Preset 3. Selection Type 4. Print List…

1. normal 2. zero 3. span 4. Add new… 5. Settings…

1. Operator PIN .... 123 2. Supervisor PIN . 135 Default settings shown

1. Header 2. Temperature 3. Quality 4. Configuration

1. Recurrance 2. Resolution 3. Appl. Correction 4. Scales 5. Temp. Comps

1. low 2. medium

1. single 2. continuous 3. auto single

1. Date Format 2. Summer Time 3. Set Clock…

1. Mode 2. Disable presser

1. Batch Codes 2. Auto Increment 3. Log Users

1. Printer 2. Serial Ports

1. Mode 2. Serial 1 3. Parallel 4. Record After Read

1. Baud Rate 2. Word Length

1. none 2. acid 3. offset

1. Add new…

1. none 5. statistics 2. printer 6. multi + prt 3. save 4. prt + save

1. none 2. 24 column 3. csv (lims)

1. 7 bit EP 2. 8 bit NP

1. 4800 2. 9600 3. 19200 4. 38400

1. off 2. on

1. off 2. number 3. text 4. dateinc 5. barcode

1. no 2. yes

1. dd.mm.yy 2. mm.dd.yy

1. english 2. french 3. spanish 4. german

1. no 2. yes

1. off 2. number 3. text

1. Use Methods If Methods are not enabled

If Methods are enabled

1. no 2. yes

Page 3-2 22-160’02 Rev. D July 2009 Setting up the system


Shortcut keys Shortcut key functions can be activated when the Measurement Display is shown. Press and hold down the key until the next screen is displayed.

Press and hold to:

switch to standby

Press and hold to: adjust the backlight and

display polarity.

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Calibration Calibration is essential to ensure accurate readings over the required measuring range and should be performed at the low end (Zero) and the high end (Span) of this range with samples of known values. Zero This can be considered as an offset to the displayed values.

Although the ideal sample for setting Zero is distilled water, any known sample at the low end of the measurement range can be used.

Span The Span can be considered as a scaling, or multiplying,

factor. Span should be checked with a known sample at the upper end of the measurement range.

Bellingham + Stanley supply a range of Calibration Materials suitable for verifying and setting the instrument calibration. See the B+S website, www.bellinghamandstanley.co.uk .

Calibration standard values

The Zero and Span aim values must be entered in the scale units selected in the measurement settings. If the instrument’s measurement settings are being set using Methods, then either the Zero or Span method will automatically be selected when a Zero or Span is being carried out. If the calibration is to be carried out at 20°C, then the specified value of the sample at 20°C should be entered. Alternatively, if the calibration is to be carried out at a temperature other than 20°C and if:

a. the sample is distilled water or a sucrose solution, then ‘sugar’ temperature compensation could be selected and the specified value of the sample at 20°C can be entered.

b. the sample is an AG Fluid, then ‘ag fluid’ temperature compensation could be selected and the specified value of the sample at 20°C can be entered.

c. the sample is not sucrose or an AG Fluid, then the specified value of the sample at the calibration temperature must be entered.

Zero calibration

press the Menu softkey select 1. Calibration select 1. Zero A message will be displayed asking for the prism to be thoroughly cleaned and the presser to be lowered. If a zero has been carried out within the last 30 minutes, the option to skip this part of the calibration is given (this allows a re-zero to be carried out without removing the sample). If the clean prism calibration is carried out, it is vital that the complete prism face is scrupulously clean and dry. Press OK.

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The Zero sample value must then be entered. If distilled water is to used and the instrument set to 20.0°C then the value will be 0.00 ºBrix or 1.33299 Refractive Index; see ‘Calibration Standard Values’ above. The current measurement settings are displayed in the bottom left of the aim point screen, so that the aim point units can be checked. Press ‘Enter’. Place a small amount of distilled water onto the Measuring Prism. The

complete prism surface must be covered by the water; generally 1ml is sufficient to achieve this.

Lower the presser. Press OK. There will then be a delay while instrument waits for the sample to

stabilise. When the calibration has been set the instrument will show ‘Calibration Complete.’.

Span calibration press the Menu softkey select 1. Calibration select 2. Span (Top Calibration) When requested, enter the Span sample value aim value. See ‘Calibration Standard Values’ above. The current measurement settings are displayed in the bottom left of the aim point screen, so that the aim point units can be checked. Press ‘Enter’.

As with the zero calibration, place the span sample onto the prism and lower the presser. Press ‘’Ok’ to start the calibration.

Continuous readings will be taken until stable and then the calibration will be corrected to the new value. During both Zero and Span calibrations, the spread of the last 5 reading values is shown in refractive index (irrespective of the scale used for calibration). This value must be less than 0.00001 for the value to be accepted.

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Cancel the last calibration If the Zero or Span calibration was carried out in error, e.g. an incorrect sample was applied or the wrong value was entered, it can be cancelled. press the Menu softkey select 1. Calibration select 3. Undo press Yes

Calibration report

Display a report of the last Zero & Span calibrations. press the Menu softkey select 1. Calibration select 4. Report

press Print to optionally send the report to a printer which can be

authorised and retained by the operator.

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Measurement settings

The instrument has the following measurement settings that will affect a reading. Scale (the scale units that the readings are displayed in) Temp. Comp. (whether temperature compensation is used) Set Temp. (temperature the instrument will control the prism to) Stability (check that the sample has stabilised) Limits (check if the reading parameters are within set limits) The measurement settings can be set in one of two ways.

Mode Menu A Mode menu is accessible from the Measurement display. The mode menu allows the operator to select the measurement settings which will be used for all subsequent readings.

Methods Preconfigured Methods, which define the measurement settings, are selected from the Measurement Display.

Which to use, the Mode menu or Methods? If the complete range of product types that is to be measured on the instrument requires the same measurement settings, then ‘Mode Menu’ option would be most suitable. The mode menu is discussed further on page 2-15. However, if a number of products with differing settings are to be read, then a method can be added for each of them. E.g. a range of soft drinks could be regularly measured in the sugar scale and the display should show whether each product reading value is within its set tolerance. The methods can be given names that are easily identifiable by the operators such as:

Method name Low limit High limit cola 10.9 11.3 lemon 9.8 10.5 orange 11.0 11.4

To enable the use of Methods press the Menu softkey select 3. Setup select 1. Methods select 1. Use Methods select 2. Yes

To disable the use of Methods (use the Mode Menu) press the Menu softkey select 3. Setup select 1. Methods select 4. Settings (Note: the selection number will change as

methods are added) select 1. Use Methods select 1. No

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Methods A ‘Method’ contains all the settings necessary for the instrument to take and check a sample reading. The settings available are: Scale (the scale units that the readings is displayed in) Temp. Comp. (whether temperature compensation is used) Set Temp. (temperature the instrument will control the prism to) Stability (check that the sample has stabilised) Limits (check if the reading parameters are within set limits) Initially, there are three methods set in the instrument: normal for measuring samples zero the conditions used for a ‘zero’ calibration span the conditions used for a ‘span’ calibration

The zero and span method only contain Scale and Temp. Comp. settings. The zero or span calibration procedure will be carried out at the temperature of the last selected method, i.e. the temperature of the last measurement reading. If the complete range of product types that is to be measured on the refractometer requires the same measurement settings then the ‘normal’ method can be selected and used for all samples. However, if a number of products with differing settings is to be checked, then a method can be added for each of them. E.g. a range of soft drinks could be regularly measured in the Brix scale and the display should show whether each product reading value is within its set tolerance. The methods can be given names that are easily identifiable by the operators such as:

Method name Low limit High limit cola 10.9 11.3 lemon 9.8 10.5 orange 11.0 11.4

Adding a new method press the Menu softkey select 3. Setup select 1. Methods

select 4. Add new… (Note: the selection number will change as methods are added)

Enter the method name. This could be a common name such as cola or lemonade, a trade name or a product reference code (but not a batch code, it must identify a generic product type). The name can be a combination of lower case letters, numbers and other characters. Set each of the following parameters for the method: Scale see page 2-16 Temp. Comp. see page 2-16 Set Temp. see page 2-17 Stability see page 3-13 Limits see page 3-12 then Quit back to the Measurement Display.

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Editing an existing method press the Menu softkey select 3. Setup select 1. Methods select the method to be edited To delete a method select option 6, Delete Method.

Hiding the preset methods

The instrument has 3 preset methods; ‘normal’, ‘zero’ and ‘span’. If the refractometer will always be used with the preset methods, i.e. one set of conditions for all samples, and no other methods have been added, then the Methods function can be disabled and the second softkey in the Measurement display will be blank. Alternatively, if a number of methods are created for a standard range of products, e.g. cola, orange, lemonade, and the preset methods will never be required, then it could be advantageous to hide ‘normal’, ‘zero’ and ‘span’ from the Methods list. This will make it easier to select the method of interest. To achieve either of these, press the Menu softkey select 3. Setup select 1. Methods

select 4. Settings (Note: the selection number will change as methods are added)

select 2. Hide Preset select 2. Yes

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Method selection type Before reading a sample, the relevant method should be selected by pressing the Method softkey in the Measurement Display. This will show a list of all available methods and the appropriate one can be selected. However, if a large number of methods have been created, e.g. greater than 20, then it could be easier to select the required method by its index number rather than scrolling through the list. To change the method selection method, press the Menu softkey select 3. Setup select 1. Methods select 4. Settings (Note: the selection number will change as

methods are added) select 3. Selection Method select list or number List selection type Number selection type

Print a list of methods To print a list of all available methods with their index numbers, press the Menu softkey select 3. Setup select 1. Methods

select 4. Settings (Note: the selection number will change as methods are added)

select 4. Print List…

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Reading limits Reading Limits allows up to three test conditions to be set. If any of the reading parameters values fall outside the limits then the reading is identified as ‘fail’. The three test conditions can be set to check any, or all, of the available parameters. These are : Reading value Temperature Quality

Adding a limits test condition Add a new method or Edit an existing method as detailed above, then select 5. Limits

select 1. Add new… (Note: the selection number will change as limits tests are added)

select the parameter 1. reading, 2. temp, 3. quality enter the Lower Limit enter the Upper Limit Example: A method has been configured with the scale set to Brix and two limits tests of: Reading value; lower limit = 10.5 upper limit = 11.5 Quality; lower limit = 105 upper limit = 110 If the Reading value is lower than 10.5 or higher than 11.5 or the Quality is less than 105 or higher than 110 then the reading will be recorded as ‘fail’ and displayed with a line

through it:

Editing or deleting a limits test condition Select an existing method as detailed above, then select 5. Limits select the Limits test to be edited change the parameter or limits as required or select 4. Delete

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Reading stability Reading Stability is a condition that must be met before a sample reading can be displayed. The Stability condition can be set to none a simple fixed delay a number of consecutive readings with a repeatable parameter value. smart detection, using both reading and temperature measurements to

determine sample stability A Reading Stability condition will only be functional while Reading Recurrence is set to Single (see page 3-20).

Adding a stability delay condition A reading Stability delay condition causes a wait period after the Read softkey has been pressed before the Read function is actually commenced. This delay provides a fixed time for the sample to stabilise on the prism and to achieve thermal equilibrium throughout the sample mass. The valid delay range is 1 to 9999 seconds. From the measurement settings select 4. Stability select 2. delay Enter a delay time in seconds

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Adding a stability repeatability condition A reading Stability repeatability condition will disable reading values being displayed until the condition is met. After the Read softkey has been pressed, the instrument will take readings until the spread of a number of consecutive parameter values do not exceed the tolerance set. The parameter could be the reading value or reading temperature or quality Examples: Three different repeatability conditions are shown below. The values displayed would be after the 3rd, 5th or 6th reading depending on which condition had been set.

From the measurement settings… select 4. Stability select 3. repeatability select the parameter 1. reading, 2. temp, 3. quality enter the tolerance that the spread of values must be within enter the number of readings that must be within tolerance.

Note: The tolerance value entered must be in the units of the selected scale for

the method; e.g. If the scale is Brix then the tolerance could be, say, 0.1 or if the scale is refractive index then the tolerance could be 0.00015.

The number of readings value must not be greater than 10. Using smart stability detection

The smart stability option takes the repeatability feature described above one stage further by testing both the reading and the temperature of the sample. The combination of both stable reading and temperature will give a good indication that the sample has stabilised. Measurements are required to be ±0.05 °C of the aim control temperature. From the measurement settings… select 4. Stability select 3. smart enter the tolerance that the spread of values must be within enter the number of readings that must be within tolerance

Note: The tolerance value entered must be in the units of the selected scale for

the method; e.g. If the scale is Brix then the tolerance could be, say, 0.1 or if the scale is refractive index then the tolerance could be 0.00015.

The number of readings value must not be greater than 10.

reading temp quality 14.70 24.84 101 14.79 24.94 104 14.81 24.98 104 14.83 25.02 104 14.82 25.00 103 14.83 24.99 104

3 reading values within 0.2 Reading 5 will be displayed

4 temp values within 0.05 Reading 6 will be displayed

2 quality values within 2 Reading 3 will be displayed

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User accessibility There are 2 PINs in this refractometer; the "operator" PIN and the "supervisor" PIN. The "operator" PIN allows access to the Mode menu (or Method selection if enabled) only and the "supervisor" which provides unrestricted operation of the Mode menu and Setup menu. Each PIN is a 3 digit number in the range 001 to 255 and is initially set as follows:- "Operator" 123 "Supervisor" 135

Entering PINs After pressing SETUP the user is prompted to enter the PIN and press Enter.

If the PIN is incorrect the instrument will return to the Measurement Display.

Changing PINs The "operator" and "supervisor" passwords can be changed to any three digit numbers in the range 001 to 255. press the Menu softkey select 3. Setup select 5. Passwords select 1. Operator PIN or 2. Supervisor PIN Setting the "operator" password to 000 will give unrestricted access to the Mode menu (or Method selection if enabled) and PIN screen will not be displayed after pressing Mode. Setting the "supervisor" password to 000 will give unrestricted access to both the Mode & Setup menus and the PIN screen will not be displayed after pressing Mode or Setup. CAUTION! Access to the MODE & SETUP functions is only possible by entering the passwords. DO NOT FORGET THEM! In the event of the PIN being forgotten, contact Bellingham & Stanley Service Department for assistance.

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System settings

Setting the date format The instrument can display the date in two formats, ‘dd.mm.yy’ (typically UK) and ‘mm.dd.yy’ (typically US). press the Menu softkey select 3. Setup select 5. System select 2. Time / Date select 1. Date Format Select the required date format from the list.

Adjust clock for summer (daylight saving) time

The instruments clock can easily be adjusted for daylight saving time (adding one hour to the clock). press the Menu softkey select 3. Setup select 5. System select 2. Time / Date select 2. Summer Time Select yes to advance the clock one hour, or no to turn back the clock one hour.

Set the clock time

press the Menu softkey select 3. Setup select 5. System select 2. Time / Date select 3. Set Clock… Enter the time and date digits as shown. The clock will be updated when the last digit is keyed.

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Set the print mode The fourth (right hand) softkey function in the Measurement Display can be set to: Mode Function Softkey

caption none None; softkey disabled blank print Print the last displayed reading to the selected

printer port Print

save Save the last displayed reading to the reading log Save prt+save Print the last displayed reading to the selected

printer port and save it to the reading log Print

statistics Allows a group of up to 10 measurements to be recorded and printed with a statistical analysis of the data (see page 3-18)

Print

multi + prt Allows a group of between 2 and 10 consecutive measurements to be read and printed automatically.

Print

To select the required mode: press the Menu softkey select 3. Setup select 5. System select 3. Communications select 1. Printer select 1. Mode

Available print formats This instrument can output data using either the serial port 1 and / or parallel port. There are 2 formats available. Format Description 24 column Optimised for use with a 24 column printer. csv (lims) Designed for easy integration with a LIM system. Each value

is separated using commas. If a printer is connected to the Parallel port then csv data can also be output via the Serial 1 port.

Select use of ports (serial 1 and parallel) If the print mode above is set to ‘printer’ then the instrument can be programmed to output data via the serial 1 and parallel port. press the Menu softkey select 3. Setup select 5. System select 3. Communications select 1. Printer select 2. Serial1 or 3. Parallel

select the desired print format When configuring the ports it is advisable to first set ‘Serial 1’ and ‘Parallel’ to

‘none’, and then configure the ports.

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Using statistic print mode The instrument has an optional statistical printout mode (see page 3-17). This allows a group of up to 10 results to be temporarily logged together and a statistical analysis carried out upon the results. When Print is pressed and no previous results have been logged, the instrument will request the traceability data (batch and operator details) if selected. This information will be used to identify all of the measurements in the group. The diagram below explains the operation of the statistical printout.

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Optional

Clears the statistics


Automatic printing/saving The instrument can be configured to automatically record a measurement following a measurement by enabling the ‘Record After Read’ option. This will, depending upon the Mode setting, print or save the result immediately after a reading. press the Menu softkey select 3. Setup select 5. System select 3. Communications select 1. Printer select 4. Record After Read Traceability options, such as batch and operator codes will be requested as if ‘Print’ or ‘Save’ had been pressed. This feature will only operate in single recurrence mode (see page 3-20).

Set the serial port configuration

The serial port baud rate and word length can be set to suit the receiving device: press the Menu softkey select 3. Setup select 5. System select 3. Communications select 2. Serial ports

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Customising the instrument The instrument can be customised to suit individual operating requirements both in visual aspects, the layout of the screen & the amount of data displayed, and operational settings.

Change the display layout press the Menu softkey select 3. Setup select 3. Display The Header, Temperature, Quality, and Configuration can be individually selected to be displayed. If all four options are de-selected then the screen will show only the reading and the softkey functions.

Change the reading recurrence press the Menu softkey select 3. Setup select 4. Reading select 1. Recurrence With Single selected, the instrument will only take a reading when the Read key is pressed and leave it on the display until Read is pressed again. Continuous mode will disable the Read key and readings will be repeatedly taken and updated on the screen. A small progress bar will be displayed about the Read key, to indicate that measurements are being taken.

If a Reading Stability condition has been set (see page 3-13), then this will be disabled while Reading Recurrence is set to Continuous. With the ‘auto single’ mode selected, the instrument will automatically take a single measurement if a sample is detected after lowering the presser.

Change the reading resolution press the Menu softkey select 3. Setup select 4. Reading select 2. Resolution Medium is the normal resolution for the instrument. Low is one digit lower resolution

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Adjust the display backlight and polarity If the display is difficult to read, especially in either uncommonly bright or dim ambient lighting conditions, it can be improved by adjusting the backlight intensity or the display polarity. The instrument must be in the Measurement Display screen. Press and hold down the ‘Methods’ or ‘Mode’ softkey for approximately three seconds. The Configure Display screen will show.

Press 1, 2 or 3 for different levels of backlight intensity. Press 0 to set the display polarity (invert the display). Press ‘OK’ to use the new settings or ‘Quit’ to revert to the previous condition.

Disabling the presser Under certain operating conditions it might be necessary to disable the presser orientation sensor. To disable the presser press the Menu softkey select 3. Setup select 5. System select 5. Temp. Control select 2. Disable Presser

If the presser has been disabled the ‘auto single’ reading recurrance mode will not function. It is advised not to disable the presser unless strictly necessary. Ambient light not shielded by the presser could cause measurement errors, and the presser insulates the measurement face from ambient temperature.

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Measurement traceability All ‘Printed’ readings are recorded together with the measurement settings selected and the time & date of the measurement. In addition, an optional product batch code and a user code can be entered.

Batch codes If a batch code is required, it can be either a number (in which case the keys will only enter 0 – 9) or as text (the keys then having full alpha-numeric capability). Setting Description off The instrument will not request a batch code. number Number from 0 to 32000. text 14 character alpha-numeric string. dateinc auto increasing number from 0 to 9999, in the format of

yymmddnnnn Example 0603117775 (11th March 2006, number 7775)

barcode first 14 characters read from a barcode (see the following page)

If number is selected, an Auto Increment option is available which will then offer a batch code which is one greater than the previous used batch code. press the Menu softkey select 3. Setup select 5. System select 4. Traceability select 1. Batch codes If the Auto Increment option is required then select 2. Auto Increment only available if Batch Codes is set to

‘number’

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Using barcodes as batch codes If a barcode reader is to be used (55-80 or 55-81) it has be to plugged in to the instrument’s serial 2 port. With the instrument showing the Measurement Display, scan in the barcode.

The display will briefly show ‘Result Printed.’ or ‘Result Saved.’ depending upon printer type configuration.

If the printer type is set to ‘printer’ then the measurement will be printed, showing the scanned barcode number.

User codes When the user code option is selected, the user can be recorded in a number of formats. press the Menu softkey select 3. Setup select 5. System select 4. Traceability select 3. Log users Setting Description off The instrument will not request a user code. number Number from 0 to 99. text 30 character alpha-numeric string.

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Adding extra scales As supplied, the instrument can display readings in refractive index or Brix scales. Up to 100 other scales can be added to convert the readings to standard recognised units, such as ºBaumé, or company specific units pertaining to a particular product.

Adding a standard scale The following standard scales are held in a library in memory.

1. zeiss 6. 90% fructose fructose vol % fsii 2. oechsle (de) 7. baumé invert sugar 3. butyro 8. sodium chloride probable alcohol 4. 42% fructose 9. oechsle (ch) urine solids 5. 55% fructose 0. glucose kmw (babo)

press the Menu softkey select 3. Setup select 4. Reading select 4. Scales select 1. Add new… select 1. From Library Select the scale required from the list. The scale will then be available when adding or editing methods.

Adding a custom designed scale

The readings can be converted to custom units using the following formula: No. Equation

1 reading = A + Bx + Cx2 + Dx3 + Ex4 + Fx5 2 available later

where: x = refractive index + Offset A,B,C,D,E,F are polynomial constants press the Menu softkey select 3. Setup select 4. Reading select 4. Scales

select 1. Add new… (Note: the selection number will change as scales are added)

select 2. From Data Enter the new scale name, (16 characters) e.g. coffee solids Enter the scale ID, (2 characters) e.g. cs Enter the 6 constants A to F. Any constants not required, (e.g. E & F with a 4 term polynomial), should be set to 0. Enter Offset (this can generally be 0 though greater accuracy can be achieved when calculating a polynomial for a range starting at water by using an Offset of –1.33) Enter the Equation no. (e.g. 1, see table above) Select the Number Format required for displaying and printing the reading. The scale will then be added to the Scales List and can subsequently be Altered, Deleted or Copied. The scale will now be available when adding or editing methods.

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Adding a custom designed temperature compensation The readings can be temperature compensated by special temperature coefficients using the following formula: No. Equation

1

Reading Correction = A + Bx + Cx2 + Dx3 + Ex4 + Fx5

2

available later where: x = sample temperature in °C A,B,C,D,E,F are polynomial constants The Reading Correction is added to the reading as refractive index, before the reading is converted to Brix or another scale. Therefore, the Reading Correction must also be calculated in refractive index. Corrected reading RI = Uncorrected reading RI + Reading Correction press the Menu softkey select 3. Setup select 4. Reading select 5. Temp. comps

select 1. Add new… (Note: the selection number will change as temp. comps are added)

Enter the new temp. comp name, (16 characters) e.g. coffee solids Enter the temp. comp ID, (2 characters) e.g. cs Enter the 6 constants A to F. Any constants not required, (e.g. E & F with a 4 term polynomial), should be set to 0. Enter the Equation no. (e.g. 1, see table above) The temp. comp will then be added to the Temp. comps List and can subsequently be Altered, Deleted or Copied. The temp. comp will now be available when adding or editing methods. Up to 16 temperature compensation modes may be added.

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Application Corrections This instrument has two special corrections that have been designed for use in specific applications, offset and acid correction. The application corrections are selected by… press the Menu softkey select 3. Setup select 4. Reading select 3. Appl. Correction Select the application correction required from the list.

Offset Correction When selected as the application correction (see above) the offset correction allows the user to easily change the reading value by adding a simple offset and scaling factor to the measurement. This can be used when it is required to adjust a refractometer to read that of a density meter. y = (x + O) × S Where y = corrected reading in user units x = reading in user units O = offset S = scaler (scaling factor) The offset and scaling factor are part of the instrument’s measurement settings, so if Methods are used each method can have its own parameters and if Methods are not used then they are set globally from the Mode menu.

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Citric Acid Correction When selected as the application correction (see page 3-26) the acid correction allows automatic correction of the effects of citric acid on the measurement. The correction conforms to BS EN 12143:1996. The percent acid value (total acid expressed as anhydrous citric acid) is part of the instrument’s measurement settings, so if Methods are used each method can have its own default acid value, and if Methods are not used then they are set globally from the Mode menu.

The acid correction is only active when the Brix scale is selected. Also be aware that the acid correction is used when calibrating the instrument, so please ensure that the percent acid value is set to 0 when calibrating. If Methods are used the zero and span methods automatically set the acid value to 0. When a measurement is printed or saved the operator is given the oportunity to change the percent acid value.

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Setting the default values If the instrument fails to start up correctly after switching on, or it is functioning unexpectedly, it could be advisable to reset certain settings to the original factory set (or default) values. Unplug the instrument. Press, and hold down, the far left soft key (power) and plug the instrument back in. Do not release the key until LED lights and then goes out. When the key is released the screen will show…

Initially the two options will be set to ‘no’ and ‘none’. Select the settings that you want to return to default values by setting them to show ‘yes’. The ‘Setup…’ option has further options within it. When set correctly press ‘Quit’ at the ‘Default Menu’.

The instrument will ask the user to confirm their choice. Selecting ‘Yes’ will carry out the default.

Displaying the software / hardware details If contacting B+S, or a local distributor regarding your instrument it is advised to have the software and hardware details to hand. This information can be viewed from the Information Menu. press the Menu softkey select 4. Information select 1. Software or 2. Hardware

Example software details Example hardware details

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Specification 22-160’02 Rev. D July 2009 Section 4

section 4

Specification Performance........................................................ 4-1 Temperature........................................................ 4-1 Temperature compensation ................................ 4-1 Prism................................................................... 4-1 Prism plate .......................................................... 4-1 RS232 configuration............................................ 4-1 Physical............................................................... 4-2 Power requirements ............................................ 4-2

Specification 22-160’02 Rev. D July 2009 Page 4-1

Specification

Performance Model RFM330+ RFM340+

Refractive index

Range minimum 1.32

Range maximum 1.58

Display resolution 0.0001 0.0001 / 0.00001

Accuracy (±) 0.0001 0.00004

Brix

Range minimum 0

Range maximum 100

Display resolution 0.1 0.1 / 0.01

Accuracy (±) 0.1 0.03

Temperature Ambient operating range 5 to 45°C Measuring low limit 0 or 10 ºC below ambient (whichever greater) Measuring upper limit 70°C Stability ±0.05°C Sensor Accuracy ±0.03°C Storage -5 to 60°C

Temperature compensation Sugar (ICUMSA) 10 to 40°C Sugar (extended) 5 to 10 and 40 to 70°C AG fluid 10 to 40°C

Prism Material Artificial sapphire ND 1.7681 Sample surface diameter 12 mm

Prism plate Material 316 stainless steel Dish diameter 59 mm Prism seal Silicone rubber and resin Spill barrier material PEEK

RS232 configuration Baud rate 4800, 9600, 19200, 38400 Word length 7 bit even parity or 8 bit no parity Default settings underlined.

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Physical Length (refractometer module only) 335 mm Width (refractometer module only) 210 mm Height (refractometer module only) 160 mm Weight (including power supply) 5.5 Kg

Power requirements Voltage 110 to 230 V~ ±10% Frequency 50 to 60 Hz Maximum current 2 A

The RFM 390+ model is a RFM300+ instrument designed to a customers specific requirements. Therefore the instruments specification may differ from those shown above. For further information please contact Bellingham + Stanley.

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Spares and accessories 22-160’02 Rev. D July 2009 Section 5

section 5

Spares and accessories Calibration AG Fluids .......................................... 5-1 Calibration Oils.................................................... 5-1 Printers................................................................ 5-1 Barcode readers.................................................. 5-1 Interconnecting cables ........................................ 5-1 Instruction manuals ............................................. 5-2 Power supplies.................................................... 5-2 Spares................................................................. 5-2 Enhanced Protection Pack .................................. 5-3 Sample contact presser insert............................. 5-4 Switch membrane anti-splash cover ................... 5-4 Waterproof Power Supply ................................... 5-4

Accessories 22-160’02 Rev. D July 2009

Page 5-1

Spares and accessories

Calibration AG Fluids AG Fluids are an organic solution calibrated in °Brix and RI, and have a long shelf life. They have no special storage or transit requirements and are therefore easy to ship and store. AG Fluids are manufactured on a %weight/weight basis in a UKAS laboratory using only the highest quality chemicals and Analar® distilled water. Manufactured in large batches, the resultant samples are checked using a controlled refractometer that has been carefully calibrated with NIST and PTB primary standards, providing further traceability.

Specification Code Type Refractive

Index* °Brix** Single 5ml

Bottle Multi-pack of 5 x 5ml Bottle

Multi-pack of 20 x 5ml Bottles

AG2.5 1.33659 2.50 90-401 90-501 90-601 AG5 1.34026 5.00 90-402 90-502 90-602 AG7.5 1.34401 7.50 90-403 90-503 90-603 AG10 1.34782 10.00 90-404 90-504 90-604 AG11.2 1.34968 11.20 90-405 90-505 90-605 AG12 1.35093 12.00 90-406 90-506 90-606 AG12.5 1.35171 12.50 90-407 90-507 90-607 AG15 1.35568 15.00 90-408 90-508 90-608 AG40 1.39986 40.00 90-418 90-518 90-618

* Refractive index @ 589.3nm & 20.0°C ** Equivalent °Brix value

Calibration Oils Calibration Oils are one of the most commonly used materials for calibrating refractometers as they have good traceability, particularly to NIST.

Specification Code Description Refractive Index* °Brix** Multi-pack of 5 x 5ml Bottles

Calibration Oil 1.46737 70.78 90-525 Calibration Oil 1.51822 90.17 90-530

* Typical Refractive Index @ 589.3nm & 20.0°C **Equivalent °Brix value @ 589.3nm & 20.0°C.

Printers Code Dot matrix impact printer parallel: Euro version 230V 55-11 Dot matrix impact printer parallel: UK version 230V 55-12 Dot matrix impact printer parallel: US version 110V 55-13 Dot matrix impact printer serial: Euro version 230V 55-14 Dot matrix impact printer serial: UK version 230V 55-15 Dot matrix impact printer serial: US version 110V 55-16 See Spares below for printer spare parts

Barcode readers Code 230V version 55-80 110V version 55-81

Interconnecting cables Code Printer serial (25way D type) 54-02 Printer parallel (25way D type to Centronics) 54-03 Computer (9way D type) 54-07

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Page 5-2 22-160’02 Rev. D July 2009 Accessories

Instruction manuals Code English Printed copy & PDF format file 22-160 French PDF format file 22-161 Spanish PDF format file 22-162 German PDF format file 22-163

Power supplies Code Power supply (unsealed) 110-230V 55-105 Mains lead for 55-105 with plug suitable for:

United Kingdom 61-191 Euro (Schuko) 61-193 United States 61-192 Switzerland 61-181 Denmark 61-182 India / South Africa 61-188 Australia 61-189 No plug – open lead 61-190

Power supply (waterproof): 110-230V without mains plug 55-250

Spares Code Sample contact presser insert 22-017 Air filter pack (standard filter) Quantity: 20 26-292 (enhanced protection filter) Quantity: 20 22-088 Paper for dot matrix impact printers Quantity: 20 55-91 Printer ribbon for dot matrix impact printers 55-93 Switch membrane anti-splash cover 26-155 Enhanced protection pack 22-80 Validation documentation (IQ OQ PQ) 22-451

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Accessories 22-160’02 Rev. D July 2009

Page 5-3

Enhanced Protection Pack - Code 22-80 The Enhanced Protection Pack comprises improved air filter holders, filters and an outlet vent cover. The pack is designed to improve protection of the instruments internal thermoelectrical temperature control components. In certain harsh environments the standard air filters and cooling air outlet vents could become covered with sample, and over time this could block the airflow, which would affect the temperature control abilities of the instrument. The Enhanced Protection Pack air filters have a greater filtering ability and are more tolerant to airbourne contaminants, which will result in longer periods between airfilter changes. The outlet vent cover stops the outlet vents from becoming blocked, if sample should run down the back of the instrument. To fit the Enhanced Protection Pack simply remove the standard side air filter holders (as if changing the filter). Replace the standard filter holders with the EPP air filter holders. Fit the outlet vent cover by pressing the cover into the outlet vent cover flange.

Please note that as the EPP is attached to the instrument by using push fittings, the instrument cannot be carried by any part of the EPP. See page 2-21 for details on changing the EPP air filter. AAcc cc

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Page 5-4 22-160’02 Rev. D July 2009 Accessories

Sample contact presser insert - Code 22-017 If the instrument is to be used with samples that are very viscose or solid the standard (non-contact) presser can be replaced with a sample contact presser insert. This insert causes a sample to be pressed against the prism when the presser is lowered. As the presser comes into contact with sample, it must be thoroughly cleaned between sample applications to stop cross contamination of samples. To fit, unscrew the four screws on the top of the presser arm. The presser insert and arm can now be separated. Fit the new presser insert, and replace the four screws to hold the insert in place.

Switch membrane anti-splash cover – Code 26-155

Splash Covers may be fitted over instrument switch panels to prevent contamination by operators when using the equipment. The covers help prevent moisture ingress and provide extra protection where sample or solvent may react with the switch membrane. The splash covers fix to the front of the instrument by adhesive strips and are made from Polyether Polyurethane.

Waterproof Power Supply - Code 55-250 A Waterproof Power Supply can be used in place of the standard Bellingham+Stanley Ltd. non-sealed unit if it is to be operated in a wet or humid environment. It is supplied with an “open-ended cable” allowing the fitment of a suitably waterproof plug – in accordance with local regulation and should be installed by a qualified person. Specification

Ambient Temperature 5 to 40°C Physical L x W x H 25 x 8 x 6 cm Rating IP 66 Voltage 100 - 240v~ ±10% Frequency 50 - 60 Hz

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