2100 expert_dna 1000_de72901983_2010-08-16_15-47-55 (testing ampure)

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Peak table for Ladder Pea k Size [bp] Conc. [ng/µl] Molarity [nmol/l] Observati ons  Area Alig ned Mig rati on Time [s] Peak Height Peak Width % of Total Time corrected area 1 15 4.20 424.2 Lower Marker 25.0 43.00 100.4 2.4 0.0 25.0 2 25 4.00 242.4 Ladder Peak 26.2 45.22 99.0 2.6 0.0 26.2 3 50 4.00 121.2 Ladder Peak 34.4 47.50 114.3 1.7 0.0 34.4 4 100 4.00 60.6 Ladder Peak 41.2 53.93 149.1 2.1 0.0 41.2 5 150 4.00 40.4 Ladder Peak 45.0 59.87 261.6 2.1 0.0 45.0 6 200 4.00 30.3 Ladder Peak 52.0 65.53 289.4 2.1 0.0 52.0 7 300 4.00 20.2 Ladder Peak 63.4 76.84 316.1 2.6 0.0 63.4 8 400 4.00 15.2 Ladder Peak 76.2 86.56 320.9 2.9 0.0 76.2 9 500 4.00 12.1 Ladder Peak 82.9 93.37 323.6 3.1 0.0 82.9 10 700 4.00 8.7 Ladder Peak 87.7 103.48 300.3 2.6 0.0 87.7 11 850 4.00 7.1 Ladder Peak 86.1 106.57 318.7 1.8 0.0 86.1 12 1,000 4.00 6.1 Ladder Peak 89.8 108.21 306.8 2.6 0.0 89.8 13 1,500 2.10 2.1 Upper Marker 52.0 113.00 154.6 2.6 0.0 52.0 Ladder 2100 Expert (B.02.07.SI532) © Copyri ght 2003 - 2009 Ag ilen t Technologies, Inc. Printed: 8/16/2010 4: 43:14 PM 2100 expert_DNA 1000_DE72901983_2010-08-16_15-4 7-55.xad Page of  1 14 Created: Modified: 8/16/2010 3:47:55 PM 8/16/2010 4:41:57 PM Data Path: DNA 1000 C:\...-16\2100 expert_DNA 1000_DE72901983_2010-08-16_15-47-55.xad  Assay Class: Electropherogram Summary

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8/8/2019 2100 expert_DNA 1000_DE72901983_2010-08-16_15-47-55 (Testing AMPure)

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Peak table for Ladder

Peak 

Size[bp]

Conc.[ng/µl]

Molarity[nmol/l]

Observations

 Area Aligned MigrationTime [s]

Peak Height

Peak Width

% of Total

Time correctedarea

1 15 4.20 424.2 LowerMarker

25.0 43.00 100.4 2.4 0.0 25.0

2 25 4.00 242.4 LadderPeak 

26.2 45.22 99.0 2.6 0.0 26.2

3 50 4.00 121.2 LadderPeak 

34.4 47.50 114.3 1.7 0.0 34.4

4 100 4.00 60.6 LadderPeak 

41.2 53.93 149.1 2.1 0.0 41.2

5 150 4.00 40.4 LadderPeak 

45.0 59.87 261.6 2.1 0.0 45.0

6 200 4.00 30.3 LadderPeak 

52.0 65.53 289.4 2.1 0.0 52.0

7 300 4.00 20.2 LadderPeak 

63.4 76.84 316.1 2.6 0.0 63.4

8 400 4.00 15.2 LadderPeak 

76.2 86.56 320.9 2.9 0.0 76.2

9 500 4.00 12.1 LadderPeak 

82.9 93.37 323.6 3.1 0.0 82.9

10 700 4.00 8.7 LadderPeak 

87.7 103.48 300.3 2.6 0.0 87.7

11 850 4.00 7.1 LadderPeak  86.1 106.57 318.7 1.8 0.0 86.1

12 1,000 4.00 6.1 LadderPeak 

89.8 108.21 306.8 2.6 0.0 89.8

13 1,500 2.10 2.1 UpperMarker

52.0 113.00 154.6 2.6 0.0 52.0

Ladder

2100 Expert (B.02.07.SI532) © Copyright 2003 - 2009 Agilent Technologies, Inc. Printed: 8/16/2010 4:43:14 PM

2100 expert_DNA 1000_DE72901983_2010-08-16_15-47-55.xad Page of  1 14

Created:Modified:

8/16/2010 3:47:55 PM8/16/2010 4:41:57 PMData Path:

DNA 1000C:\...-16\2100 expert_DNA 1000_DE72901983_2010-08-16_15-47-55.xad

 Assay Class:

Electropherogram Summary

8/8/2019 2100 expert_DNA 1000_DE72901983_2010-08-16_15-47-55 (Testing AMPure)

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Peak table for sample 1 : Preclearing - 25bp Ladder (1)

Peak 

Size[bp]

Conc.[ng/µl]

Molarity[nmol/l]

Observations

 Area Aligned MigrationTime [s]

Peak Height

Peak Width

% of Total

Timecorrected area

1 15 4.20 424.2 LowerMarker

55.3 43.00 86.7 2.6 0.0 140.3

2 374 0.40 1.6 14.0 84.05 29.0 0.6 0.7 15.4

3 489 1.43 4.4 56.7 92.64 110.8 0.8 2.9 55.7

4 555 1.33 3.6 54.1 96.18 68.3 1.6 2.8 50.9

5 633 2.69 6.4 112.3 100.11 136.6 1.1 5.8 100.9

6 652 1.93 4.5 80.8 101.07 130.7 0.8 4.2 71.9

7 675 7.80 17.5 329.0 102.22 352.8 1.4 17.0 288.8

8 780 7.51 14.6 315.9 105.13 436.3 1.2 16.3 268.7

9 838 6.47 11.7 270.2 106.32 312.2 1.0 14.0 227.0

10 910 5.40 9.0 228.7 107.23 253.9 1.2 11.8 190.2

11 1,076 7.14 10.1 315.2 108.94 218.3 2.2 16.3 257.6

12 1,292 3.45 4.0 158.9 111.01 146.6 1.9 8.2 127.2

13 1,500 2.10 2.1 UpperMarker

100.6 113.00 103.4 1.6 0.0 79.0

Preclearing - 25bp Ladder (1)

Overall Results for sample 1 : Preclearing - 25bp Ladder (1)

Number of peaks found: 11

2100 Expert (B.02.07.SI532) © Copyright 2003 - 2009 Agilent Technologies, Inc. Printed: 8/16/2010 4:43:14 PM

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Created:Modified:

8/16/2010 3:47:55 PM8/16/2010 4:41:57 PMData Path:

DNA 1000C:\...-16\2100 expert_DNA 1000_DE72901983_2010-08-16_15-47-55.xad

 Assay Class:

Electropherogram Summary Continued ...

8/8/2019 2100 expert_DNA 1000_DE72901983_2010-08-16_15-47-55 (Testing AMPure)

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Peak table for sample 2 : Preclearing - 25bp Ladder (2)

Peak 

Size[bp]

Conc.[ng/µl]

Molarity[nmol/l]

Observations

 Area Aligned MigrationTime [s]

Peak Height

Peak Width

% of Total

Timecorrected area

1 15 4.20 424.2 LowerMarker

59.1 43.00 90.6 2.7 0.0 150.3

2 369 0.47 1.9 16.6 83.53 30.6 0.8 0.7 18.5

3 374 0.79 3.2 28.2 84.01 42.5 1.2 1.1 31.2

4 489 2.63 8.2 105.9 92.66 150.4 2.3 4.3 104.7

5 556 1.20 3.3 49.5 96.19 65.6 2.1 2.0 46.9

6 635 5.05 12.1 212.7 100.17 200.4 1.7 8.6 192.5

7 651 3.93 9.1 166.3 101.02 202.9 1.0 6.7 149.1

8 676 11.19 25.1 476.8 102.26 441.4 2.5 19.3 421.8

9 782 10.66 20.7 452.8 105.16 534.1 1.6 18.3 388.2

10 842 6.29 11.3 265.3 106.41 314.1 1.0 10.7 224.4

11 908 5.20 8.7 222.7 107.21 246.2 1.1 9.0 186.8

12 1,063 7.20 10.3 320.2 108.82 212.3 2.2 13.0 264.2

13 1,290 3.28 3.9 152.8 110.99 144.0 1.8 6.3 123.3

14 1,500 2.10 2.1 UpperMarker

101.7 113.00 102.4 1.9 0.0 80.5

Preclearing - 25bp Ladder (2)

Overall Results for sample 2 : Preclearing - 25bp Ladder (2)

Number of peaks found: 12

2100 Expert (B.02.07.SI532) © Copyright 2003 - 2009 Agilent Technologies, Inc. Printed: 8/16/2010 4:43:14 PM

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Created:Modified:

8/16/2010 3:47:55 PM8/16/2010 4:41:57 PMData Path:

DNA 1000C:\...-16\2100 expert_DNA 1000_DE72901983_2010-08-16_15-47-55.xad

 Assay Class:

Electropherogram Summary Continued ...

8/8/2019 2100 expert_DNA 1000_DE72901983_2010-08-16_15-47-55 (Testing AMPure)

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Peak table for sample 3 : Preclearing - 1Kb Ladder (1)

Peak 

Size[bp]

Conc.[ng/µl]

Molarity[nmol/l]

Observations

 Area Aligned MigrationTime [s]

Peak Height

Peak Width

% of Total

Timecorrected area

1 15 4.20 424.2 LowerMarker

56.4 43.00 92.2 2.0 0.0 144.4

2 454 0.09 0.3 13.9 90.24 22.3 1.3 13.2 15.4

3 480 0.01 0.0 2.5 91.99 3.1 1.8 2.4 2.7

4 906 0.50 0.8 88.8 107.18 115.0 2.5 84.4 81.8

5 1,500 2.10 2.1 UpperMarker

423.2 113.00 490.2 2.6 0.0 368.5

Preclearing - 1Kb Ladder (1)

Overall Results for sample 3 : Preclearing - 1Kb Ladder (1)

Number of peaks found: 3

2100 Expert (B.02.07.SI532) © Copyright 2003 - 2009 Agilent Technologies, Inc. Printed: 8/16/2010 4:43:14 PM

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Created:Modified:

8/16/2010 3:47:55 PM8/16/2010 4:41:57 PMData Path:

DNA 1000C:\...-16\2100 expert_DNA 1000_DE72901983_2010-08-16_15-47-55.xad

 Assay Class:

Electropherogram Summary Continued ...

8/8/2019 2100 expert_DNA 1000_DE72901983_2010-08-16_15-47-55 (Testing AMPure)

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Peak table for sample 4 : Preclearing - 1Kb Ladder (2)

Peak 

Size[bp]

Conc.[ng/µl]

Molarity[nmol/l]

Observations

 Area Aligned MigrationTime [s]

Peak Height

Peak Width

% of Total

Timecorrected area

1 15 4.20 424.2 LowerMarker

55.8 43.00 91.7 1.9 0.0 144.7

2 455 0.08 0.3 12.4 90.33 20.4 1.3 12.2 14.0

3 481 0.00 0.0 0.0 92.07 -0.4 1.9 0.0 0.0

4 908 0.51 0.9 89.4 107.20 117.3 2.7 87.8 83.8

5 1,500 2.10 2.1 UpperMarker

416.1 113.00 499.7 3.2 0.0 368.9

Preclearing - 1Kb Ladder (2)

Overall Results for sample 4 : Preclearing - 1Kb Ladder (2)

Number of peaks found: 3

2100 Expert (B.02.07.SI532) © Copyright 2003 - 2009 Agilent Technologies, Inc. Printed: 8/16/2010 4:43:14 PM

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Created:Modified:

8/16/2010 3:47:55 PM8/16/2010 4:41:57 PMData Path:

DNA 1000C:\...-16\2100 expert_DNA 1000_DE72901983_2010-08-16_15-47-55.xad

 Assay Class:

Electropherogram Summary Continued ...

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2nd Binding Sup - 25bp Ladder (1)

2100 Expert (B.02.07.SI532) © Copyright 2003 - 2009 Agilent Technologies, Inc. Printed: 8/16/2010 4:43:14 PM

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Created:Modified:

8/16/2010 3:47:55 PM8/16/2010 4:41:57 PMData Path:

DNA 1000C:\...-16\2100 expert_DNA 1000_DE72901983_2010-08-16_15-47-55.xad

 Assay Class:

Electropherogram Summary Continued ...

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2nd Binding Sup - 25bp Ladder (2)

2100 Expert (B.02.07.SI532) © Copyright 2003 - 2009 Agilent Technologies, Inc. Printed: 8/16/2010 4:43:14 PM

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Created:Modified:

8/16/2010 3:47:55 PM8/16/2010 4:41:57 PMData Path:

DNA 1000C:\...-16\2100 expert_DNA 1000_DE72901983_2010-08-16_15-47-55.xad

 Assay Class:

Electropherogram Summary Continued ...

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2nd Binding Sup - 1Kb Ladder (1)

2100 Expert (B.02.07.SI532) © Copyright 2003 - 2009 Agilent Technologies, Inc. Printed: 8/16/2010 4:43:14 PM

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Created:Modified:

8/16/2010 3:47:55 PM8/16/2010 4:41:57 PMData Path:

DNA 1000C:\...-16\2100 expert_DNA 1000_DE72901983_2010-08-16_15-47-55.xad

 Assay Class:

Electropherogram Summary Continued ...

8/8/2019 2100 expert_DNA 1000_DE72901983_2010-08-16_15-47-55 (Testing AMPure)

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2nd Binding Sup - 1Kb Ladder (2)

2100 Expert (B.02.07.SI532) © Copyright 2003 - 2009 Agilent Technologies, Inc. Printed: 8/16/2010 4:43:14 PM

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Created:Modified:

8/16/2010 3:47:55 PM8/16/2010 4:41:57 PMData Path:

DNA 1000C:\...-16\2100 expert_DNA 1000_DE72901983_2010-08-16_15-47-55.xad

 Assay Class:

Electropherogram Summary Continued ...

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Peak table for sample 9 : Elute (End-repaired DNA) - 25bp Ladder (1)

Peak 

Size[bp]

Conc.[ng/µl]

Molarity[nmol/l]

Observations

 Area Aligned MigrationTime [s]

Peak Height

Peak Width

% of Total

Timecorrected area

1 15 4.20 424.2 LowerMarker

64.8 43.00 108.4 2.4 0.0 171.8

2 250 1.71 10.4 54.3 71.24 80.7 2.1 73.2 84.6

3 505 0.31 0.9 14.1 93.64 13.2 2.5 19.0 16.6

4 734 0.10 0.2 4.7 104.18 7.9 1.7 6.3 5.0

5 1,042 0.02 0.0 1.1 108.61 3.0 2.1 1.5 1.1

6 1,500 2.10 2.1 UpperMarker

114.2 113.00 131.3 5.9 0.0 110.7

7 1,807 0.00 0.0 17.7 115.94 21.8 2.0 0.0 16.7

8 2,198 0.00 0.0 38.5 119.68 18.7 12.3 0.0 35.2

Elute (End-repaired DNA) - 25bp Ladder (1)

Overall Results for sample 9 : Elute (End-repaired DNA) - 25bp Ladder (1)

Number of peaks found: 4

2100 Expert (B.02.07.SI532) © Copyright 2003 - 2009 Agilent Technologies, Inc. Printed: 8/16/2010 4:43:14 PM

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Created:Modified:

8/16/2010 3:47:55 PM8/16/2010 4:41:57 PMData Path:

DNA 1000C:\...-16\2100 expert_DNA 1000_DE72901983_2010-08-16_15-47-55.xad

 Assay Class:

Electropherogram Summary Continued ...

8/8/2019 2100 expert_DNA 1000_DE72901983_2010-08-16_15-47-55 (Testing AMPure)

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Peak table for sample 10 : Elute (End-repaired DNA) - 25bp Ladder (2)

Peak 

Size[bp]

Conc.[ng/µl]

Molarity[nmol/l]

Observations

 Area Aligned MigrationTime [s]

Peak Height

Peak Width

% of Total

Timecorrected area

1 15 4.20 424.2 LowerMarker

66.2 43.00 109.2 2.6 0.0 174.5

2 249 1.80 11.0 62.7 71.06 93.6 1.8 100.0 97.2

3 1,500 2.10 2.1 UpperMarker

125.6 113.00 147.9 2.4 0.0 120.4

4 1,814 0.00 0.0 25.3 116.01 27.5 2.2 0.0 23.6

5 2,200 0.00 0.0 22.8 119.70 26.6 1.7 0.0 20.6

Elute (End-repaired DNA) - 25bp Ladder (2)

Overall Results for sample 10 : Elute (End-repaired DNA) - 25bp Ladder (2)

Number of peaks found: 1

2100 Expert (B.02.07.SI532) © Copyright 2003 - 2009 Agilent Technologies, Inc. Printed: 8/16/2010 4:43:14 PM

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Created:Modified:

8/16/2010 3:47:55 PM8/16/2010 4:41:57 PMData Path:

DNA 1000C:\...-16\2100 expert_DNA 1000_DE72901983_2010-08-16_15-47-55.xad

 Assay Class:

Electropherogram Summary Continued ...

8/8/2019 2100 expert_DNA 1000_DE72901983_2010-08-16_15-47-55 (Testing AMPure)

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Peak table for sample 11 : Elute (End-repaired DNA) - 1Kb Ladder (1)

Peak 

Size[bp]

Conc.[ng/µl]

Molarity[nmol/l]

Observations

 Area Aligned MigrationTime [s]

Peak Height

Peak Width

% of Total

Timecorrected area

1 15 4.20 424.2 LowerMarker

69.2 43.00 111.9 2.3 0.0 180.8

2 179 0.02 0.2 0.6 63.16 2.6 2.1 1.0 1.0

3 205 0.06 0.4 1.8 66.09 4.4 1.6 3.1 3.0

4 230 0.09 0.6 2.9 68.96 6.2 1.6 4.9 4.6

5 256 0.09 0.6 3.3 71.84 6.8 1.4 5.5 4.9

6 281 0.13 0.7 4.7 74.66 9.0 2.1 8.0 6.9

7 305 0.04 0.2 1.7 77.37 7.1 0.7 2.8 2.3

8 332 0.09 0.4 3.7 79.93 7.2 1.4 6.2 5.0

9 358 0.08 0.3 3.4 82.44 6.2 1.5 5.7 4.4

10 382 0.10 0.4 4.4 84.79 7.9 1.6 7.5 5.7

11 406 0.09 0.3 4.0 86.99 7.2 1.4 6.7 5.0

12 465 0.08 0.3 4.0 91.01 6.3 2.1 6.8 4.8

13 520 0.45 1.3 22.2 94.40 32.1 1.8 37.4 25.4

14 556 0.05 0.1 2.7 96.18 3.1 1.7 4.4 3.0

15 1,500 2.10 2.1 UpperMarker

123.9 113.00 148.1 3.3 0.0 117.6

Elute (End-repaired DNA) - 1Kb Ladder (1)

Overall Results for sample 11 : Elute (End-repaired DNA) - 1Kb Ladder (1)

Number of peaks found: 13

2100 Expert (B.02.07.SI532) © Copyright 2003 - 2009 Agilent Technologies, Inc. Printed: 8/16/2010 4:43:14 PM

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Created:Modified:

8/16/2010 3:47:55 PM8/16/2010 4:41:57 PMData Path:

DNA 1000C:\...-16\2100 expert_DNA 1000_DE72901983_2010-08-16_15-47-55.xad

 Assay Class:

Electropherogram Summary Continued ...

8/8/2019 2100 expert_DNA 1000_DE72901983_2010-08-16_15-47-55 (Testing AMPure)

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Peak table for sample 12 : Elute (End-repaired DNA) - 1Kb Ladder (2)

Peak 

Size[bp]

Conc.[ng/µl]

Molarity[nmol/l]

Observations

 Area Aligned MigrationTime [s]

Peak Height

Peak Width

% of Total

Timecorrected area

1 15 4.20 424.2 LowerMarker

71.6 43.00 114.2 2.7 0.0 185.2

2 179 0.03 0.2 1.0 63.15 2.9 0.6 1.4 1.8

3 205 0.01 0.1 0.6 66.06 3.9 0.2 0.8 0.9

4 230 0.06 0.4 2.6 68.96 6.0 1.0 3.6 4.1

5 256 0.07 0.4 3.1 71.82 7.0 1.2 4.4 4.6

6 280 0.04 0.2 1.9 74.62 8.3 0.3 2.6 2.7

7 304 0.07 0.4 3.4 77.27 7.4 1.2 4.8 4.8

8 331 0.08 0.3 3.8 79.87 7.5 1.4 5.4 5.2

9 357 0.07 0.3 3.8 82.41 7.3 1.5 5.3 4.9

10 381 0.09 0.4 5.1 84.70 9.3 1.0 7.2 6.5

11 406 0.07 0.3 3.9 86.93 6.9 1.4 5.4 4.8

12 436 0.08 0.3 4.7 89.01 7.4 1.7 6.7 5.7

13 465 0.07 0.2 4.3 90.98 7.2 1.4 6.0 5.1

14 519 0.45 1.3 28.2 94.36 41.8 1.8 39.7 32.0

15 554 0.07 0.2 4.6 96.12 4.7 2.0 6.7 5.1

16 1,500 2.10 2.1 UpperMarker

155.1 113.00 186.4 3.1 0.0 146.3

17 2,162 0.00 0.0 11.4 119.34 13.3 2.5 0.0 10.2

Elute (End-repaired DNA) - 1Kb Ladder (2)

Overall Results for sample 12 : Elute (End-repaired DNA) - 1Kb Ladder (2)

Number of peaks found: 14

2100 Expert (B.02.07.SI532) © Copyright 2003 - 2009 Agilent Technologies, Inc. Printed: 8/16/2010 4:43:14 PM

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Created:Modified:

8/16/2010 3:47:55 PM8/16/2010 4:41:57 PMData Path:

DNA 1000C:\...-16\2100 expert_DNA 1000_DE72901983_2010-08-16_15-47-55.xad

 Assay Class:

Electropherogram Summary Continued ...

8/8/2019 2100 expert_DNA 1000_DE72901983_2010-08-16_15-47-55 (Testing AMPure)

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2100 Expert (B.02.07.SI532) © Copyright 2003 - 2009 Agilent Technologies, Inc. Printed: 8/16/2010 4:43:14 PM

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Created:Modified:

8/16/2010 3:47:55 PM8/16/2010 4:41:57 PMData Path:

DNA 1000C:\...-16\2100 expert_DNA 1000_DE72901983_2010-08-16_15-47-55.xad

 Assay Class:

Gel Image