2017 iowa evaluation of insecticides and plant ... · plant-incorporated protectants corn pests...

IOWA STATE UNIVERSITY OF SCIENCE AND TECHNOLOGY

2017 IOWA EVALUATION OF INSECTICIDES

AND

PLANT-INCORPORATED PROTECTANTS

CORN PESTS RESEARCH PROJECT

DEPARTMENT OF ENTOMOLOGY

AMES, IOWA 50011-3140

SUE BLODGETT, CHAIR

INSECT INVESTIGATED

Corn Rootworm

Diabrotica virgifera virgifera and Diabrotica barberi

Report Authors March 30, 2018

Aaron Gassmann File number: 303-17

Patrick Weber

2017 IOWA EVALUATION OF INSECTICIDES AND

PLANT INCORPORATED PROTECTANTS

TABLE OF CONTENTS Page 1. Research Personnel .......................................... 1 2. Introduction and Objective ............................... 2 3. Testing Procedures and Evaluations ............... 2 4. Results and Discussion ................................... 5 5. Studies Conducted ............................................ 9 6. Corn Rootworm Evaluation Tables Ames (Johnson Farm) Insecticide and Bt traits Root Injury/Percent Consistency ........... 10 Stand Count .......................................... 10 Yield ...................................................... 11 Insecticide on non-Bt corn Root Injury/Percent Consistency ........... 12 Stand Count .......................................... 13 Yield ...................................................... 14 Boone (Bruner Farm) Insecticide on non-Bt corn Root Injury/Percent Consistency ........... 15 Stand Count .......................................... 16 Yield ...................................................... 17

Table of Contents (Continued) Page Crawfordsville Insecticide on non-Bt corn Study #1 Root Injury/Percent Consistency ........... 17 Stand Count .......................................... 18 Yield ...................................................... 18 Insecticide on non-Bt corn Study #2 Root Injury/Percent Consistency ........... 19 Stand Count .......................................... 20 Yield ...................................................... 21 Nashua Insecticide on Bt corn Root Injury/Percent Consistency ........... 22 Stand Count .......................................... 22 Yield ...................................................... 23 Insecticide on non-Bt corn Root Injury/Percent Consistency ........... 24 Stand Count .......................................... 24 Yield ...................................................... 25

7. Appendix I - Agronomic Information ............. 26 8. Appendix II – Weather Data ............................ 29 9. Appendix III - Materials Tested ...................... 39

10. Appendix IV - Research Pictures .................. 42

2017 IOWA EVALUATION OF INSECTICIDES AND

PLANT INCORPORATED PROTECTANTS

CORN PESTS RESEARCH PROJECT

DEPARTMENT OF ENTOMOLOGY

AMES, IOWA 50011-3140

Project Leader: Aaron Gassmann

Agricultural Specialist: Patrick Weber

Postdoctoral Research Associate: Ram Shrestha Research Associate Ben Brenizer Graduate Research Assistants: Mattea Allert Coy St. Clair Eric Clifton Abigail Kropf Eric Yu Technical Assistants: Luwis Andradi Bryan Hall Christophe Petit Ethan Rose Kelli Steinlage University Research Farm Superintendents: Kent Berns Mike Fiscus Dallas Maxwell Kenneth Pecinovsky Myron Rees Terry Tuttle

2

INTRODUCTION In Iowa, the species of corn rootworm (CRW) that are of economic importance include western corn rootworm Diabrotica virgifera virfigera LeConte and northern corn rootworm D. barberi Smith and Lawrence, and these two species are the most damaging pests of corn, Zea mays, in the Unites States Corn Belt. Eggs are laid in the soil during the summer and hatch the following spring. Larval feeding on corn roots in June may diminish yield by reducing plant growth and drought tolerance, and by imposing harvest losses due to plant lodging. Adult emergence from the soil is underway by early July and continues through late summer. Additional crop losses may be caused by adult beetles feeding on corn silk and soft doughy kernels. In Iowa, crop rotation, where it fits cropping practices, remains the preferred method of management. However, it is also economically feasible to protect corn roots with soil-applied insecticides and plant incorporated protectants, which are transgenic corn hybrids that produce insecticidal toxins derived from Bacillus thuringiensis (Bt). In additional to corn rootworm, several aboveground lepidopteran pests feed on corn in Iowa. These include the European corn borer Ostrinia nubilalis, black cutworm Agrotis ipsilon, fall armyworm Spodoptera frugiperda, and corn earworm Helicoverpa zea. Over the last two decades, Bt corn varieties have been made commercially available to protect corn from these pests.

OBJECTIVE The goal of this research program is to serve the agricultural community of Iowa

by monitoring and evaluating the performance of registered and pre-commercial insecticides and transgenic corn hybrids. To achieve this goal, we maintain a viable, proactive, progressive and scientifically sound product evaluation program.

TESTING PROCEDURES AND EVALUATIONS

Field Sites: Research plots were established at four Iowa State University research farms in 2017: 1) Johnson Farm (Ames), 2) Bruner Farm (Boone), 3) Northeast Research and Demonstration Farm (Nashua) and 4) Southeast Research and Demonstration Farm (Crawfordsville). At all locations, two sections of the study site are rotated annually as either research plots or late-planted trap crop. The seed planted for the trap crop is a mixed maturity blend with a greater proportion of late-maturing varieties. This trap crop constitutes a favorable environment for adult female CRW late in the season when other fields are maturing, and can result in a high abundance of CRW larvae the following season. Table 1 lists the studies conducted at each location.

METHODS

Field plot design: For all locations, the experimental design was a randomized complete block with four replications. Field plots varied in length from 35 to 75 feet. Planting: Seeds were pre-bagged in the laboratory and then planted with a four-row John Deere Max EmergeTM 7100 Integral Rigid Frame Planter that had 30 inch row spacing. Seeds were planted at a depth of 2 inches with a spacing of 0.6

3

inches between seeds (35,600 seeds per acre). The standard planter fiberglass seed hoppers with attached finger-pickup mechanism were replaced with modified units. On these modified units, the metal plate that covered the fingers had been replaced with a 7/8 inch clear Plexiglas plate. Inserted through the Plexiglas was a small stainless-steel cylinder. The cylinder was positioned to deliver seed to the pickup fingers. At the beginning of each replication, pre-bagged seeds were dumped into the steel cylinder. At the beginning of each replication, a hydraulic motor (attached to the planter’s drive shaft) was activated to deliver seed immediately into the ground. At the end of each replication, this same hydraulic motor was activated to expel any unplanted seed, and the finger pickup mechanism was visually inspected through the clear Plexiglas plate to ensure that no seed was present.

Planting of studies that only evaluated a single hybrid was accomplished with a two-row John Deere planter that had 30 inch row spacing and bulk seed hoppers. Seeds were planted at a depth of 2 inches with a spacing of 0.6 inches between seeds (35,600 seeds per acre).

Plant-incorporated protectants: Plant-incorporated protectants were evaluated in corn hybrids producing insecticidal toxins derived from the bacterium Bacillus thuringiensis (Bt), and these are described in Appendix III. Seed treatments: All the DeKalb hybrids were treated with Poncho 250 or 500. All the Pioneer hybrids were

treated with either Cruiser 250 + Maxim Quattro + Poncho1250/Votivo + PPCT12/13, or Cruiser 250 + Maxim Quattro + PPCT2013. These seed treatments target secondary pests, such as wireworm and seed corn maggot but may have some, limited efficacy against CRW larave at levels higher than 250 mg active ingredient per kernel. Liquid soil-applied insecticides: Capture LFR 1.5SC, Force 250CS, Force HL 10G, Index 2.80 CS , Venerate XC and MBI-305BM1SE1 were applied in-furrow at planting with a compressed-air system built directly into the planter by Almaco manufacturing (Nevada, IA). The products Capture LFR 1.5SC, Force 250CS, Force HL 10G, Index 2.80 CS were applied as ounces per 1000 row feet. Venerate XC and MBI-305BM1SE1 were applied as gallons per acre. All liquid insecticide was applied with a Teejet XR80015EVS spray nozzles at 21 psi to deliver 5 GPA of finished spray at a tractor speed of 4 mph.

Capture LFR 1.5SC, Force 250CS, Force HL 10G, Index 2.80CS, Venerate XC and MBI-305BM1SE1 used water as the carrier.

Before the field plot season, two new spray nozzles were installed per row (T-Band & In-Furrow) and calibrated with water to ensure proper application of product.

For these liquid applications each row nozzle was checked for product, correct spray pattern prior to plot application and monitored during application to ensure that insecticides were applied correctly. Final incorporation was

4

accomplished with drag chains mounted behind the closing wheels.

Liquid commercial products applied including Capture LFR 1.5SC, Capture 3RIVE 3D 1.6SC, Ethos XB 1.5SC, Force 250CS, Puma, Temitry LFR. Capture 3RIVE 3D 1.6SC and Force 250CS used water as the carrier. The products Ethos XB 1.5SC, Temitry LFR, Puma and Capture LFR used starter fertilizer (Nucleus Hyperlink HP 8-24-4) as a carrier.

Liquid non-commercial products applied in the three studies included F-4022-1, F-4120-2, F-4260-2, F-4270-7, F-4260-7 3RIVE, F-4274-3, and F-4278-3. The products (F-4120-2, F-4022-1, F-4260-7, F-4260-7 3RIVE, F-4278-2) used water as the carrier. The products F-4022-1, F-4260-2 and F-4274-3 used starter fertilizer (Nucleus Hyperlink HP 8-24-4) as a carrier.

These studies were planted with a two row John Deere 7100 planter that had 30 inch row spacing. All insecticides were applied as fluid ounces per 1000 row feet. All these products were applied in-furrow at a tractor speed of 3 mph.

SmartBoxTM application of soil-applied insecticides: Aztec-HC 9.34G and SmartChoice 15G insecticide treatments were applied with modified SmartBoxTM metering units mounted on the planter. The commercial SmartBoxTM units were removed from their large-base containers and sandwiched between a flat metal plate on the bottom and a custom-made, threaded plastic cap on the top. The bottom plate had been fabricated so that

it could slide in and out of the same planter mounting brackets used for the Noble units. An inverted 1 L Nalgene bottle attached to the top provided a secure and sealed container for insecticide for the SmartBoxTM units. Clear plastic tubes directed the granular insecticides to both the in-furrow and T-band placement. A controller mounted in the tractor cab was used to operate all the SmartBoxTM metering units. These units were managed using the AMVAC SmartBox System Manager. All treatments were applied at 4 mph using the “fixed speed mode” on the SmartBoxTM controllers. These SmartBoxTM metering units were calibrated before going to the field to deliver ounces of product per 1000 row feet.

General methodology: During planting, each row was constantly monitored to ensure that insecticides were applied correctly. Final incorporation was accomplished with drag chains mounted behind the closing wheels.

Evaluations

Stand counts: These were measured by laying a stand count chain length 17’ 5” long (1/1000 of an acre for 30-inch row spacing) between the two corn rows and counting the number of plants in both rows. Late season stand counts were measured following the same procedure as early season stand counts but using a two-inch PVC pipe cut to the length of 17’5” long. Measurements for both dates were averaged to provide a single value for stand counts. Root injury: After the majority of corn rootworms had finished feeding, roots

5

were dug to assess feeding injury. This took place from July 24 to August 11, 2017. Prior to leaving the field, excess soil was removed from roots and all roots were labeled with study name and plot number using a Sharpie Fine Point permanent marker. Roots were cleaned at the ISU Johnson Farm’s root washing station. These roots were first soaked in water for 2 hours and then washed with a hose to remove any remaining soil. Roots were evaluated for rootworm feeding injury following the Iowa State 0 to 3 Node-Injury Scale: Node-Injury Scale (0-3): 0.00 - No feeding injury (lowest rating

that can be given). 1.0 - One node (circle of roots), or the

equivalent of an entire node, pruned to within 1.5 inches of the stalk or soil line.

2.00 - Two nodes pruned. 3.00 - Three or more nodes pruned.

(Highest rating that can be given) Injury in between complete nodes pruned is noted as the percentage of the node missing (e.g., 1.50 = one and a half nodes pruned and 0.25 = one quarter of one node pruned). The linear node-injury scale allows injury to be expressed intuitively and has proved useful in evaluating minor injury.

For an interactive guide to the node-injury scale, see the website for the Department of Entomology at Iowa State University.

http://www.ent.iastate.edu/dept/faculty/gassmann/rootworm

Yields: Studies that were taken to yield were machine harvested with a modified John Deere 9410 or 9450 plot combine owned by Iowa State University. Weight (pounds) and percent moisture were recorded with either a HarvestMaster brand or Shivvers brand 5010 moisture meter and Avery-WeighTronix weigh system/915 weigh scale indicator harvest data collection system. These measurements were converted to bushels per acre of No. 2 shelled corn (56 lbs. per bushel) at 15.5% moisture in Microsoft Excel.

Data analysis

Data were analyzed with analysis of variance (ANOVA) procedures using SAS 9.4. When a significant treatment effect was present pairwise comparisons made among means with an experiment wise error rate of P < 0.05.

Product consistency: Percent product consistency was calculated as the percentage of times a treatment limited feeding injury to 0.25 node or less (greater injury can result in economic yield loss, especially when plants are moisture stressed).

Results and Discussion In 2017, feeding pressure from corn rootworm was light to moderate, with generally less than a node of injury to the untreated checks across studies at the various research farms. The one exception was a study evaluating soil-insecticide on non-Bt corn at the Bruner Farm in central Iowa, where the untreated check suffered more than 2.5 nodes of injury.

http://www.ent.iastate.edu/dept/faculty/gassmann/rootworm

6

For the study evaluating soil-applied insecticide on Bt corn, which was conducted at the Johnson Research Farm in central Iowa (Tables 2 to 4), feeding injury was fairly light, with the untreated check only suffering 0.64 nodes of root injury. Both Bt rootworm traits and soil-insecticide applied to non-Bt corn significantly reduced root injury compared to the untreated check. However, the addition of a soil-applied insecticide to a Bt rootworm hybrid did not lead to a significant further reduction in root injury (Table 2). Similarly, product consistency was significantly higher for either rootworm-traited Bt corn or non-Bt corn with soil-applied insecticide, when compared to the untreated check, but product consistency was not significantly improved by adding soil-applied insecticide to rootworm-traited Bt corn. Some variation in stand counts were noted among treatments (Table 3). In general treatments yielded well in this study, with many treatment exceeding 200 bu/acre. The untreated check yielded only 165 bu/acre, illustrating the potential for even modest rootworm injury to reduce yield in some cases. Both non-rootworm Bt corn with soil-applied insecticide and Bt-rootworm traited corn had significantly greater yield than the untreated check (Table 4). A second study conducted at the Johnson Research Farm measured effects of soil-applied insecticide on non-rootworm Bt corn (Tables 5 -7). In this study, he untreated check suffered only 0.33 nodes of injury and none of the treatment had significantly lower

injury than the check (Table 5). No significant differences were present for product consistency or stand counts (Tables 5 and 6). All treatments had significantly higher yield than the untreated check and some significant differences were detected (Table 7). The Bruner Research Farm in central Iowa had the highest larval feeding pressure of any study evaluated in 2017. In a study evaluating larval injury to non-rootworm Bt corn with soil-applied insecticide, the untreated check had the highest level of injury at 2.69 nodes (Table 8). Several of the insecticides tested significantly reduced root injury but overall product consistency in this study was very low (Table 8). No significant differences were found for stand counts (Table 9) or yield (Table 10). For one of two studies evaluating soil-applied insecticide on non-Bt corn in southeastern Iowa (Tables 11 to 13), rootworm feeding pressure was very light, and no differences were found among treatments for any of the variables measured (Table 11). Average root injury never exceeded 0.25 nodes for any of the treatments evaluated or for the untreated check. Similarly, no differences were observed for product consistency or stand counts (Tables 11 and 12). In general, yields were good and ranged from 247 to 276 bushels per acre. No significant differences in yield were observed among treatments (Table 13). The other study conducted in 2017 at the Southeast Research and Demonstration Farm also measured

7

soil-applied insecticide on non-rootworm traited corn (Tables 14 to 17). Rootworm pressure was light with the untreated check only suffering 0.43 nodes of injury (Table 14). In general, soil-applied insecticides reduced root injury but few significant differences were present. Yields ranged from 170 to 231 bushels per acre but were not improved by the addition of insecticide, likely due to the low rootworm pressure for this study (Table 16) In general, the pressure was light for the study conducted at the Northeastern Research and Demonstration Farm, in Nashua, IA testing soil-applied insecticide on Bt corn (Tables 17 -19), with the untreated check suffering only less than half a node of injury. We found that all Bt hybrids significantly reduced injury compared to the untreated check. For the Bt hybrids evaluated, the addition of soil-applied insecticide did not significantly reduce root injury. Index, soil-applied insecticide, on non-Bt corn had a similar level of root injury to the Bt hybrids. It is worth noting that all the Bt hybrids evaluated in this study contained the Cry34/35Ab1 trait. While resistance to Cry3Bb1 corn by western corn rootworm is widespread, resistance to Cry34/35Ab1 appears to be limited in distribution and incomplete, and this likely contributed to the high level of root protection observed for Bt hybrids in this study. Yield was similar among treatments, which was probably due in part to the low overall level of rootworm pressure in this study. The numerically highest yield was for a non-RW Bt hybrid with soil-applied insecticide.

A second study conducted at the Northeast Research and Demonstration Farm tested the effects of soil-applied insecticide to non-rootworm Bt corn (Tables 20 to 22). The untreated check suffered 0.83 nodes of injury (Table 20). While injury was numerically lower for all insecticide treated hybrids, no significant differences were detected. Similarly, no significant differences were present in either stand counts (Table 21) or yield (Table 22).

ADDITIONAL INFORMATION Agronomic information and field history are listed in Appendix I. Weather data from the test site or the nearest Iowa climatological station are listed in Appendix II. Information on materials tested is listed in Appendix III. Pictures of various research activities are in Appendix IV.

RESEARCH SUPPORT Support for this research was provided by the Iowa State University Agriculture and Home Economics Experiment Station and by the following companies: AMVAC, Dow, FMC, Marrone Bio Innovations, Monsanto, and Syngenta.

WAIVER OF ENDORSEMENT

This report deals with the relative ability of each treatment to protect corn from damage by insects. This information is not presented to endorse the use of any product and the name of Iowa State University should not appear in any advertising without prior written consent. Iowa State University, their respective officers, agents, or employees, have not made, and do not hereby make, any representation, warranty or covenant

8

with respect to the use of these test results, nor will they be liable for any damages, losses, or claims, including those of an incidental or consequential nature, arising out of the use of these test results.

9

Table 1. Iowa evaluation of insecticides and plant-incorporated protectants.

1 All these studies were conducted following corn rootworm trap crop, to ensure the presence of rootworm larvae in the study.

Experimental Unit Size

Table Entries/ Rows/ Row # Test Location Type of Studies1 Numbers Test Plot Length (ft) Reps

Ames-Johnson Farm 1) Insecticide and Bt traits

2) Insecticide on non-Bt corn 2-4 5-7

12 2 14 4

75 40

4 4

Boone-Bruner Farm 1) Insecticide on non-Bt corn

8-10 14 4 40 4

Crawfordsville 1) Insecticide on non-Bt corn (study #1)

2) Insecticide on non-Bt corn (study #2)

11-13 14-16

7 2 14 4

35

40

4

4

Nashua 1) Insecticide on Bt corn 2) Insecticide on non-Bt corn

17-19 20-22

12 4 14 2

75 25

4 4

10

Table 2. Average Root Injury and Product Consistency for Insecticide and Bt traits: Johnson Farm, Ames.1

Node- Product

Treatment2 Form. Rate3 Placement4 Injury5,6,7 Consistency7,8

Pioneer AMXT + Index 2.80CS 0.27 Furrow 0.07a 100a Pioneer AMXT + Aztec-HC 9.34G 0.14 Furrow-SB 0.09ab 100a DeKalb SSTX RIB + Aztec-HC 9.34G 0.14 Furrow-SB 0.10ab 85a Pioneer AMX + Aztec-HC 9.34G 0.14 Furrow-SB 0.12ab 95a DeKalb SSTX RIB + Index 2.80CS 0.27 Furrow 0.15ab 85a DeKalb SSTX RIB ----- ----- ----- 0.15ab 90a Pioneer AMXT ----- ----- ----- 0.15ab 95a Pioneer AMX + Index 2.80CS 0.27 Furrow 0.16ab 85a DeKalb non-RW Bt + Aztec-HC 9.34G 0.14 Furrow-SB 0.23ab 70a Pioneer AMX ----- ----- ----- 0.32 b 60ab DeKalb non-RW Bt + Index 2.80CS 0.27 Furrow 0.37ab 75a DeKalb non-RW Bt ----- ----- ----- 0.64 c 40 b

1 Planted May 15, 2017; evaluated July 31, 2017

2 non-RW Bt = an absence of any Bt trait targeting corn rootworm; DeKalb non-RW Bt = DeKalb brand VT2P (DKC 62-98); DeKalb SSTX RIB = DeKalb brand Smartstax RIB (DKC 50-82); Pioneer AMX = Pioneer AcreMax Xtra (P0157AMX); Pioneer AMXT = Pioneer AcreMax Xtreme (P0339AMXT) 3 Insecticide listed as ounces a.i. per 1,000 row-feet 4 Furrow-SB = insecticide applied with SmartBox system at planting time; Furrow = insecticide applied at planting time 5 Chemical and check means based on 20 observations (5 roots/2 rows x 4 replications) 6 Iowa State Node-Injury Scale (0-3). Number of full or partial nodes completely eaten 7 Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05) 8 Product consistency = Percentage of times nodal injury was 0.25 (¼ node eaten) or less

Table 3. Average Stand Count for Insecticide and Bt Traits: Johnson Farm, Ames.1

Stand

Treatment2 Form. Rate3 Placement4 Counts5,6

DeKalb SSTX RIB ----- ----- ----- 32.75a DeKalb non-RW Bt + Aztec-HC 9.34G 0.14 Furrow-SB 32.50a DeKalb SSTX RIB + Index 2.80CS 0.27 Furrow 32.25a DeKalb non-RW Bt ----- ----- ----- 31.50a DeKalb non-RW Bt + Index 2.80CS 0.27 Furrow 31.50a DeKalb SSTX RIB + Aztec-HC 9.34G 0.14 Furrow-SB 31.50a Pioneer AMX ----- ----- ----- 31.50a Pioneer AMX + Index 2.80CS 0.27 Furrow 31.25a Pioneer AMX + Aztec-HC 9.34G 0.14 Furrow-SB 30.75ab Pioneer AMXT + Index 2.80CS 0.27 Furrow 30.25ab Pioneer AMXT + Aztec-HC 9.34G 0.14 Furrow-SB 29.00 b Pioneer AMXT ----- ----- ----- 28.75 b

1 Planted May 15, 2017; evaluated May 5 and October 12, 2017

2 non-RW Bt = an absence of any Bt trait targeting corn rootworm; DeKalb non-RW Bt = DeKalb brand VT2P (DKC 62-98); DeKalb SSTX RIB = DeKalb brand Smartstax RIB (DKC 50-82); Pioneer AMX = Pioneer AcreMax Xtra (P0157AMX); Pioneer AMXT = Pioneer AcreMax Xtreme (P0339AMXT) 3 Insecticide listed as ounces a.i. per 1,000 row-feet 4 Furrow-SB = Insecticide applied with SmartBox system at planting time; Furrow = insecticide applied at planting time 5 Chemical and check means based on 16 observations (2 rows/ treatment x 17.4 row-ft/treatment x 4 replications x 2 evaluation

dates) 6 Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05)

11

Table 4. Average Yield for Insecticide and Bt traits: Johnson Farm, Ames.1

Bushels/

Treatment2 Form. Rate3 Placement4 Acre5,6,7

Pioneer AMXT + Index 2.80CS 0.27 Furrow 230a DeKalb non-RW Bt + Index 2.80CS 0.27 Furrow 222ab Pioneer AMX + Index 2.80CS 0.27 Furrow 212ab Pioneer AMXT ----- ----- ----- 212ab Pioneer AMXT + Aztec-HC 9.34G 0.14 Furrow-SB 208abc Pioneer AMX ----- ----- ----- 205abc Pioneer AMX + Aztec-HC 9.34G 0.14 Furrow-SB 199abc DeKalb non-RW Bt + Aztec-HC 9.34G 0.14 Furrow-SB 199abc DeKalb SSTX RIB + Index 2.80CS 0.27 Furrow 186abc DeKalb SSTX RIB + Aztec-HC 9.34G 0.14 Furrow-SB 185abc DeKalb SSTX RIB ----- ----- ----- 180 bc DeKalb non-RW Bt ----- ----- ----- 165 c

1 Planted May 15, 2017; machine harvested October 23, 2017 2 non-RW Bt = an absence of any Bt trait targeting corn rootworm; DeKalb non-RW Bt = DeKalb brand VT2P (DKC 62-98); DeKalb SSTX RIB = DeKalb brand Smartstax RIB (DKC 50-82); Pioneer AMX = Pioneer AcreMax Xtra (P0157AMX); Pioneer AMXT = Pioneer AcreMax Xtreme (P0339AMXT) 3 Insecticide listed as ounces a.i. per 1,000 row-feet 4 Furrow-SB = insecticide applied with SmartBox system at planting time; Furrow = insecticide applied at planting time 5 Chemical and check means based on 4 observations (2-row treatment x 68 row-feet/treatment x 4 replications) 6 Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05) 7 Yields converted to 15.5% Moisture

12

Table 5. Average Root Injury and Product Consistency for Insecticide on Non-Bt corn: Johnson Farm, Ames1

Node- Product

Treatment2 Form. Rate3 Placement4 Injury 5,6,7 Consistency8,9

DeKalb non-RW Bt + Temitry LFR 2.0SL 0.148 Furrow 0.15a 90 DeKalb non-RW Bt + F4274-3 200SC 0.12 Furrow 0.17a 75 DeKalb non-RW Bt + Capture LFR 1.5SC 0.10 Furrow 0.18a 85 DeKalb non-RW Bt + Force 250CS 0.12 Furrow 0.18a 80 DeKalb non-RW Bt + F-4022-1 + Puma 1.5SC 0.10+4.0 Furrow 0.21a 85 DeKalb non-RW Bt + F4260-7/Capture 3RIVE 3D 0.03+0.10 Furrow 0.25a 80 DeKalb non-RW Bt + Capture 3RIVE 3D 1.6SC 0.10 Furrow 0.26ab 65 DeKalb non-RW Bt + F4278-2 ` 400SL 0.13 Furrow 0.26ab 75 DeKalb non-RW Bt + F4022-1 1.6SC 0.10 Furrow 0.30ab 65 DeKalb non-RW Bt ----- ----- ----- 0.33ab 70 DeKalb non-RW Bt + F4260-7 3RIVE 3D 240SC 0.04 Furrow 0.39ab 45 DeKalb non-RW Bt + Ethos XB 1.5SC 0.10 Furrow 0.47ab 60 DeKalb non-RW Bt + F4120-2 20.67SC 0.10 Furrow 0.51ab 65 DeKalb non-RW Bt + F4260-2 200SC 0.04 Furrow 0.59 b 35

1 Planted May 15, 2017 evaluated August 10, 2017 2 non-RW Bt = an absence of any Bt trait targeting corn rootworm; DeKalb non-RW Bt = DeKalb brand VT2P RIB (DeKalb 62-98); All treatments included starter fertilizer as a carrier except F4120-2, F-4260-7, F4278-2, F-4260-7 3RIVE 3D, Force and Capture 3RIVE 3D treatments which used water as a carrier 3 All Insecticides listed as ounces a.i. per 1,000 row-feet except product Puma listed as ounces per acre

4 Furrow = insecticide applied at planting time 5 Chemical and check means based on 20 observations (5 roots/2 rows x 4 replications) 6 Iowa State Node-Injury scale (0-3). Number of full or partial nodes completely eaten 7 Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05) 8 Product consistency = Percentage of times nodal injury was 0.25 (¼ node eaten) or less

9 No significant differences between means (ANOVA, P < 0.05)

13

Table 6. Average Stand Count for Insecticide on Non-Bt Corn: Johnson Farm, Ames1

Stand


DeKalb non-RW Bt + F-4022-1 + Puma 1.5SC 0.10+4.0 Furrow 34.00 DeKalb non-RW Bt + F4260-2 200SC 0.04 Furrow 33.25 DeKalb non-RW Bt + F4274-3 200SC 0.12 Furrow 33.25 DeKalb non-RW Bt + Ethos XB 1.5SC 0.10 Furrow 33.00 DeKalb non-RW Bt + F4260-7 3RIVE 3D 240SC 0.04 Furrow 33.00 DeKalb non-RW Bt ----- ----- ----- 32.75 DeKalb non-RW Bt + Force 250CS 0.12 Furrow 32.75 DeKalb non-RW Bt + F4022-1 1.6SC 0.10 Furrow 32.50 DeKalb non-RW Bt + Capture 3RIVE 3D 1.6SC 0.10 Furrow 32.50 DeKalb non-RW Bt + F4260-7+Capture 3RIVE 3D 0.03+0.10 Furrow 32.50 DeKalb non-RW Bt + Capture LFR 1.5SC 0.10 Furrow 32.25 DeKalb non-RW Bt + F4278-2 400SL 0.13 Furrow 32.25 DeKalb non-RW Bt + Temitry LFR 2.0SL 0.148 Furrow 32.00 DeKalb non-RW Bt + F4120-2 20.67SC 0.10 Furrow 32.00

1 Planted May 15, 2017; evaluated June 5 and October 12, 2017 2 non-RW Bt = an absence of any Bt trait targeting corn rootworm; DeKalb non-RW Bt = DeKalb brand VT2P RIB (DeKalb 62-98); All treatments included starter fertilizer as a carrier except F4120-2, F-4260-7, F4278-2, F-4260-7 3RIVE 3D, Force and Capture 3RIVE 3D treatments which used water as a carrier 3 All Insecticides listed as ounces a.i. per 1,000 row-feet except product Puma listed as ounces per acre

4 Furrow = insecticide applied at planting time 5 Chemical and check means based on 16 observations (2-row treatment x 17.4 row-feet/treatment x 4 replications x 2 evaluation dates) 6 No significant differences between means (ANOVA, P < 0.05)

14

Table 7. Average Yield for Insecticide on Non-Bt Corn: Johnson Farm, Ames1

Bushels/

Treatment2 Form. Rate3 Rate3

Placement4 Acre5,6,7

DeKalb non-RW Bt + F4022-1 1.6SC 0.10 Furrow 239a DeKalb non-RW Bt + Temitry LFR 2.0SL 0.148 Furrow 236ab DeKalb non-RW Bt + F4274-3 200SC 0.13 Furrow 235ab DeKalb non-RW Bt + F-4022-1 + Puma 1.5SC 0.10+4.0 Furrow 233ab DeKalb non-RW Bt + Capture 3RIVE 3D 1.6SC 0.10 Furrow 232ab DeKalb non-RW Bt + F4120-2 20.67SC 0.10 Furrow 232ab DeKalb non-RW Bt + Capture LFR 1.5SC 0.10 Furrow 225ab DeKalb non-RW Bt + Ethos XB 1.5SC 0.10 Furrow 224ab DeKalb non-RW Bt + F4278-2 400SL 0.13 Furrow 223ab DeKalb non-RW Bt + F4260-7 3RIVE 3D 240SC 0.04 Furrow 222ab DeKalb non-RW Bt + F4260-7+Capture 3RIVE 3D 0.03+0.10 Furrow 220ab DeKalb non-RW Bt + Force 250CS 0.12 Furrow 220ab DeKalb non-RW Bt + F4260-2 200SC 0.04 Furrow 220ab DeKalb non-RW Bt ----- ----- ----- 204 b

1 Planted May 15, 2017; Machine harvested October 23, 2017 2 non-RW Bt = an absence of any Bt trait targeting corn rootworm; DeKalb non-RW Bt = DeKalb brand VT2P RIB (DeKalb 62-98); All treatments included starter fertilizer as a carrier except F4120-2, F-4260-7, F4278-2, F-4260-7 3RIVE 3D, Force and Capture 3RIVE 3D treatments which used water as a carrier 3 All Insecticides listed as ounces a.i. per 1,000 row-feet except product Puma listed as ounces per acre 4 Furrow = insecticide applied at planting time 5 Chemical and check means based on 4 observations (4-row treatment x 34 row-feet/treatment x 4 replications) 6 Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05) 7 Yields converted to 15.5% Moisture

15

Table 8. Average Root-Injury and Product Consistency for Insecticide on Non-Bt Corn: Bruner Farm, Boone1

Node- Product

Treatment2 Form.

Form. Rate3 Placement4 Injury 5,6,7 Consistency8,9

DeKalb non-RW Bt + Force 250CS 0.12 Furrow 0.84a 20 DeKalb non-RW Bt + F4022-1 1.6SC 0.10 Furrow 1.27ab 10 DeKalb non-RW Bt + F-4022-1 + Puma 1.5SC 0.10+4.0 Furrow 1.44 bc 0 DeKalb non-RW Bt + Capture 3RIVE 3D 1.6SC 0.10 Furrow 1.46 bc 0 DeKalb non-RW Bt + F4274-3 200SC 0.12 Furrow 1.48 bc 15 DeKalb non-RW Bt + Temitry LFR 2.0SL 0.148 Furrow 1.49 bc 0 DeKalb non-RW Bt + F4278-2 400SL 0.13 Furrow 1.62 bc 0 DeKalb non-RW Bt + Ethos XB 1.5SC 0.10 Furrow 1.80 bc 10 DeKalb non-RW Bt + F4120-2 20.67SC 0.10 Furrow 1.91 bcd 0 DeKalb non-RW Bt + Capture LFR 1.5SC 0.10 Furrow 1.96 cd 0 DeKalb non-RW Bt + F4260-7 3RIVE 3D 240SC 0.04 Furrow 2.06 cd 0 DeKalb non-RW Bt + F4260-7/Capture 3RIVE 3D 0.03+0.10 Furrow 2.17 cd 0 DeKalb non-RW Bt + F4260-2 200SC 0.04 Furrow 2.25 cd 0 DeKalb non-RW Bt ----- ----- ----- 2.69 d 0

1 Planted May 15, 2017 evaluated August 14, 2017 2 non-RW Bt = an absence of any Bt trait targeting corn rootworm; DeKalb non-RW Bt = DeKalb brand VT2P RIB (DeKalb 62-98); All treatments included starter fertilizer as a carrier except F4120-2, F-4260-7, F4278-2, F-4260-7 3RIVE 3D, Force and Capture 3RIVE 3D treatments which used water as a carrier 3 All Insecticides listed as ounces a.i. per 1,000 row-feet except product Puma listed as ounces per acre


9 No significant differences between means (ANOVA, P < 0.05)

16

Table 9. Average Stand Count for Insecticide on non-Bt Corn: Bruner Farm, Boone1

Stand


DeKalb non-RW Bt + F4022-1 1.6SC 0.10 Furrow 33.75 DeKalb non-RW Bt + F4260-2 200SC 0.04 Furrow 33.50 DeKalb non-RW Bt + Capture LFR 1.5SC 0.10 Furrow 33.25 DeKalb non-RW Bt + F-4022-1 + Puma 1.5SC 0.10+4.0 Furrow 33.25 DeKalb non-RW Bt + F4274-3 200SC 0.12 Furrow 33.00 DeKalb non-RW Bt + F4260-7 3RIVE 3D 240SC 0.04 Furrow 32.50 DeKalb non-RW Bt + F4260-7+Capture 3RIVE 3D 0.03+0.10 Furrow 32.25 DeKalb non-RW Bt + Capture 3RIVE 3D 1.6SC 0.10 Furrow 32.25 DeKalb non-RW Bt + F4278-2 400SL 0.13 Furrow 32.25 DeKalb non-RW Bt + Force 250CS 0.12 Furrow 32.25 DeKalb non-RW Bt + F4120-2 20.67SC 0.10 Furrow 32.00 DeKalb non-RW Bt ----- ----- ----- 32.00 DeKalb non-RW Bt + Ethos XB 1.5SC 0.10 Furrow 31.50 DeKalb non-RW Bt + Temitry LFR 2.0SL 0.148 Furrow 31.50

1 Planted May 15, 2017; evaluated June 5 and October 12, 2017 2 non-RW Bt = an absence of any Bt trait targeting corn rootworm; DeKalb non-RW Bt = DeKalb brand VT2P RIB (DeKalb 62-98); All treatments included starter fertilizer as a carrier except F4120-2, F-4260-7, F4278-2, F-4260-7 3RIVE 3D, Force and Capture 3RIVE 3D treatments which used water as a carrier

3 All Insecticides listed as ounces a.i. per 1,000 row-feet except product Puma listed as ounces per acre

4 Furrow = insecticide applied at planting time 5 Chemical and check means based on 16 observations (2-row treatment x 17.4 row-feet/treatment x 4 replications x 2 evaluation dates) 6 No significant differences between means (ANOVA, P < 0.05)

17

Table 10. Average Yield for Insecticide on Non-Bt Corn: Bruner Farm, Boone1

Bushels/



DeKalb non-RW Bt + F4260-2 200SC 0.04 Furrow 168 DeKalb non-RW Bt + F4278-2 400SL 0.13 Furrow 161 DeKalb non-RW Bt + Ethos XB 1.5SC 0.10 Furrow 155 DeKalb non-RW Bt + F4274-3 200SC 0.12 Furrow 154 DeKalb non-RW Bt + Capture 3RIVE 3D 1.6SC 0.10 Furrow 152 DeKalb non-RW Bt + Temitry LFR 2.0SL 0.148 Furrow 149 DeKalb non-RW Bt + F4022-1 1.6SC 0.10 Furrow 149 DeKalb non-RW Bt + Force 250CS 0.12 Furrow 148 DeKalb non-RW Bt + F-4022-1 + Puma 1.5SC 0.10+4.0 Furrow 146 DeKalb non-RW Bt + F4120-2 20.67SC 0.10 Furrow 146 DeKalb non-RW Bt + F4260-7 3RIVE 3D 240SC 0.04 Furrow 143 DeKalb non-RW Bt + Capture LFR 1.5SC 0.10 Furrow 139 DeKalb non-RW Bt ----- ----- ----- 137 DeKalb non-RW Bt + F4260-7+Capture 3RIVE 3D 0.03+0.10 Furrow 135

1 Planted May 15, 2017; Machine harvested October 26, 2017 2 non-RW Bt = an absence of any Bt trait targeting corn rootworm; DeKalb non-RW Bt = DeKalb brand VT2P RIB (DeKalb 62-98); All treatments included starter fertilizer as a carrier except F4120-2, F-4260-7, F4278-2, F-4260-7 3RIVE 3D, Force and Capture 3RIVE 3D treatments which used water as a carrier 3 All Insecticides listed as ounces a.i. per 1,000 row-feet except product Puma listed as ounces per acre 4 Furrow = insecticide applied at planting time 5 Chemical and check means based on 4 observations (4-row treatment x 34 row-feet/treatment x 4 replications) 6 No significant differences between means (ANOVA, P < 0.05) 7 Yields converted to15.5% Moisture Table 11. Average Root Injury and Product Consistency for Insecticide on Non-Bt Corn (Study #1): Southeast Iowa

Research and Demonstration Farm, Crawfordsville1

Node- Product


DeKalb non-RW Bt + Aztec-HC 9.34G 0.14 Furrow-SB 0.07 100 DeKalb non-RW Bt + Force 250CS 0.12 Furrow 0.08 100 DeKalb non-RW Bt + SmartChoice-HC 15G 0.14 Furrow-SB 0.15 95 DeKalb non-RW Bt + Force HL 10G 0.14 Furrow 0.16 80 DeKalb non-RW Bt ----- ----- ----- 0.16 85 DeKalb non-RW Bt + Index 2.80CS 0.27 Furrow 0.18 85 DeKalb non-RW Bt + Capture LFR 1.5SC 0.10 Furrow 0.19 80

1 Planted May 9, 2017; evaluated July 26, 2017

2 non-RW Bt = an absence of any Bt trait targeting corn rootworm; DeKalb non-RW Bt = DeKalb brand VT2P (DKC 62-98 VT2 RIB) 3 Insecticide listed as ounces a.i. per 1,000 row-feet 4 Furrow-SB = insecticide applied with SmartBox system at planting time; Furrow = insecticide applied at planting time 5 Chemical and check means based on 20 observations (5 roots/2 rows x 4 replications) 6 Iowa State Node-Injury Scale (0-3). Number of full or partial nodes completely eaten 7 No significant differences between means (ANOVA, P < 0.05) 8 Product consistency = Percentage of times nodal injury was 0.25 (¼ node eaten) or less 9 No significant differences between means (ANOVA, P < 0.05)

18

Table 12. Average Stand Count for Insecticide on Non-Bt Corn (Study #1): Southeast Iowa Research and Demonstration

Farm, Crawfordsville1

Stand


DeKalb non-RW Bt + Index 2.8CS 0.27 Furrow 30.25 DeKalb non-RW Bt + Capture LFR 1.5SC 0.10 Furrow 30.00 DeKalb non-RW Bt + Aztec-HC 9.34G 0.14 Furrow-SB 30.00 DeKalb non-RW Bt + Force HL 10G 0.14 Furrow 29.50 DeKalb non-RW Bt + Force 250CS 0.12 Furrow 29.50 DeKalb non-RW Bt + SmartChoice-HC 15G 0.14 Furrow-SB 29.25 DeKalb non-RW Bt ----- ----- ----- 29.25

1 Planted May 9, 2017; evaluated June 2 and October 24, 2017

2 non-RW Bt = an absence of any Bt trait targeting corn rootworm; DeKalb non-RW Bt = DeKalb brand VT2P (DKC 62-98 VT2 RIB) 3 Insecticide listed as ounces a.i. per 1,000 row-feet 4 Furrow-SB = Insecticide applied with SmartBox system at planting time; Furrow = insecticide applied at planting time 5 Chemical and check means based on 16 observations (2 rows/ treatment x 17.4 row-ft/treatment x 4 replications x 2 evaluation dates) 6 No significant differences between means (ANOVA, P < 0.05)

Table 13. Average Yield for Insecticide on Non-Bt Corn (Study #1): Southeast Iowa Research and Demonstration Farm,

Crawfordsville1

Treatment2 Form. Rate3 Placement4 Yield5,6

DeKalb non-RW Bt + Index 2.8CS 0.27 Furrow 276 DeKalb non-RW Bt + Capture LFR 1.5SC 0.10 Furrow 275 DeKalb non-RW Bt + Aztec-HC 9.34G 0.14 Furrow-SB 275 DeKalb non-RW Bt + Force HL 10G 0.14 Furrow 270 DeKalb non-RW Bt ----- ----- ----- 261 DeKalb non-RW Bt + SmartChoice-HC 15G 0.14 Furrow-SB 259 DeKalb non-RW Bt + Force 250CS 0.12 Furrow 247

1 Planted May 9, 2017; machine harvested October 24, 2017 2 non-RW Bt = an absence of any Bt trait targeting corn rootworm; DeKalb non-RW Bt = DeKalb brand VT2P (DKC 62-98 VT2 RIB) 3 Insecticide listed as ounces a.i. per 1,000 row-feet 4 Furrow-SB = insecticide applied with SmartBox system at planting time; Furrow = insecticide applied at planting time 5 Chemical and check means based on 4 observations (2-row treatment x 33 row-feet/treatment x 4 replications) 6 No significant differences between means (ANOVA, P < 0.05)

19

Table 14. Average Root Injury and Product Consistency for Insecticide on Non-Bt Corn (Study #2): Southeast Iowa

Research and Demonstration Farm, Crawfordsville1

Node- Product

Treatment2 Form.

Form Rate3 Placement4 Injury 5,6,7 Consistency8,9

DeKalb non-RW Bt + F4022-1 1.6SC 0.10 Furrow 0.18a 90a DeKalb non-RW Bt + F4274-3 200SC 0.12 Furrow 0.18ab 90a DeKalb non-RW Bt + Force 250CS 0.12 Furrow 0.19ab 75ab DeKalb non-RW Bt + Ethos XB 1.5SC 0.10 Furrow 0.20ab 85ab DeKalb non-RW Bt + F4278-2 400SL 0.13 Furrow 0.21ab 80ab DeKalb non-RW Bt + Temitry LFR 2.0SL 0.148 Furrow 0.23ab 75ab DeKalb non-RW Bt + Capture LFR 1.5SC 0.10 Furrow 0.26abc 65ab DeKalb non-RW Bt + F4260-7 3RIVE 3D 240SC 0.04 Furrow 0.30abc 50ab DeKalb non-RW Bt + F4260-7+Capture 3RIVE 3D 0.03+0.10 Furrow 0.31abc 60ab DeKalb non-RW Bt + F4120-2 20.67SC 0.10 Furrow 0.32abc 55ab DeKalb non-RW Bt + Capture 3RIVE 3D 1.6SC 0.10 Furrow 0.32abc 55ab DeKalb non-RW Bt + F-4022-1 + Puma 1.5SC 0.10+4.0 Furrow 0.34abc 45ab DeKalb non-RW Bt ----- ----- ----- 0.43 bc 50ab DeKalb non-RW Bt + F4260-2 200SC 0.04 Furrow 0.49 c 35ab

1 Planted May 9, 2017 evaluated August 8 & 9, 2017 2 non-RW Bt = an absence of any Bt trait targeting corn rootworm; DeKalb non-RW Bt = DeKalb brand VT2P RIB (DeKalb 62-98); All treatments included starter fertilizer as a carrier except F4120-2, F-4260-7, F4278-2, F-4260-7 3RIVE 3D, Force and Capture 3RIVE 3D treatments which used water as a carrier 3 All Insecticides listed as ounces a.i. per 1,000 row-feet except product Puma listed as ounces per acre


9 Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05)

20

Table 15. Average Stand Count for Insecticide on Non-Bt Corn (Study #2): Southeast Iowa Research and Demonstration

Farm, Crawfordsville1

Stand


DeKalb non-RW Bt + Temitry LFR 2.0SL 0.148 Furrow 32.25a DeKalb non-RW Bt + Capture 3RIVE 3D 1.6SC 0.10 Furrow 30.50a DeKalb non-RW Bt ----- ----- ----- 30.50a DeKalb non-RW Bt + Force 250CS 0.12 Furrow 30.25a DeKalb non-RW Bt + F4260-2 + Puma 1.5SC 0.10+4.0 Furrow 29.50a DeKalb non-RW Bt + F4260-7/Capture 3RIVE 3D 0.03+0.10 Furrow 29.50a DeKalb non-RW Bt + Capture LFR 1.5SC 0.10 Furrow 29.25a DeKalb non-RW Bt + F4022-1 1.6SC 0.10 Furrow 29.00a DeKalb non-RW Bt + F4260-7 3RIVE 3D 240SC 0.04 Furrow 28.75a DeKalb non-RW Bt + Ethos XB 1.5SC 0.10 Furrow 28.50a DeKalb non-RW Bt + F4278-2 400SL 0.13 Furrow 28.50a DeKalb non-RW Bt + F4274-3 200SC 0.12 Furrow 28.00a DeKalb non-RW Bt + F4120-2 20.67SC 0.10 Furrow 22.50 b

1 Planted May 9, 2017; evaluated June 2 and October 24, 2017 2 non-RW Bt = an absence of any Bt trait targeting corn rootworm; DeKalb non-RW Bt = DeKalb brand VT2P RIB (DeKalb 62-98); All treatments included starter fertilizer as a carrier except F4120-2, F-4260-7, F4278-2, F-4260-7 3RIVE 3D, Force and Capture 3RIVE 3D treatments which used water as a carrier 3 All Insecticides listed as ounces a.i. per 1,000 row-feet except product Puma listed as ounces per acre

4 Furrow = insecticide applied at planting time 5 Chemical and check means based on 16 observations (2-row treatment x 17.4 row-feet/treatment x 4 replications x 2 evaluation dates) 6 Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05)

21

Table 16. Average Yield for Insecticide on Non-Bt Corn (Study #2): Southeast Iowa Research and Demonstration Farm,

Crawfordsville1

Bushels/



DeKalb non-RW Bt + F4022-1 1.6SC 0.10 Furrow 231a DeKalb non-RW Bt + Temitry LFR 2.0SL 0.148 Furrow 228a DeKalb non-RW Bt ----- ----- ----- 224a DeKalb non-RW Bt + Force 250CS 0.12 Furrow 218ab DeKalb non-RW Bt + Capture LFR 1.5SC 0.10 Furrow 212ab DeKalb non-RW Bt + F4260-7/Capture 3RIVE 3D 0.03+0.10 Furrow 211ab DeKalb non-RW Bt + Capture 3RIVE 3D 1.6SC 0.10 Furrow 210ab DeKalb non-RW Bt + F4260-2 200SC 0.02 Furrow 209ab DeKalb non-RW Bt + F4278-2 400SL 0.13 Furrow 206ab DeKalb non-RW Bt + F4260-7 3RIVE 3D 240SC 0.04 Furrow 200ab DeKalb non-RW Bt + F-4022-1 + Puma 1.5SC 0.10+4.0 Furrow 197ab DeKalb non-RW Bt + Ethos XB 1.5SC 0.10 Furrow 193ab DeKalb non-RW Bt + F4274-3 200SC 0.12 Furrow 190ab DeKalb non-RW Bt + F4120-2 20.67SC 0.10 Furrow 170 b

1 Planted May 9, 2017; Machine harvested October 24, 2017 2 non-RW Bt = an absence of any Bt trait targeting corn rootworm; DeKalb non-RW Bt = DeKalb brand VT2P RIB (DeKalb 62-98); All treatments included Starter fertilizer as a carrier except F4120-2, F-4260-7, F4278-2, F-4260-7 3RIVE 3D, Force and Capture 3RIVE 3D treatments which used water as a carrier 3 All Insecticides listed as ounces a.i. per 1,000 row-feet except product Puma listed as ounces per acre 4 Furrow = insecticide applied at planting time 5 Chemical and check means based on 4 observations (4-row treatment x 34 row-feet/treatment x 4 replications) 6 Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05) 7 Yields converted to15.5% Moisture

22

Table 17. Average Root Injury and Product Consistency for Insecticide on Bt Corn: Northeast Iowa Research and

Demonstration Farm, Nashua1

Node- Product


Pioneer AMXT + Aztec-HC 9.34G 0.14 Furrow-SB 0.03a 100a Pioneer AMXT + Index 2.80CS 0.27 Furrow 0.03a 100a Pioneer AMXT ----- ----- ----- 0.04a 100a DeKalb SSTX RIB + Aztec-HC 9.34G 0.14 Furrow-SB 0.04a 100a DeKalb SSTX RIB ----- ----- ----- 0.05a 100a Pioneer AMX + Index 2.80CS 0.27 Furrow 0.05a 100a DeKalb SSTX RIB + Index 2.80CS 0.27 Furrow 0.05a 95ab Pioneer AMX + Aztec-HC 9.34G 0.14 Furrow-SB 0.07a 95ab Pioneer AMX ----- ----- ----- 0.14a 90ab DeKalb non-RW Bt + Index 2.80CS 0.27 Furrow 0.14ab 90ab DeKalb non-RW Bt ----- ----- ----- 0.31 bc 70ab DeKalb non-RW Bt + Aztec-HC 9.34G 0.14 Furrow-SB 0.41 c 65 b

1 Planted May 4, 2017; evaluated August 4, 2017

2 non-RW Bt = an absence of any Bt trait targeting corn rootworm; DeKalb non-RW Bt = DeKalb brand VT2P (DKC 62-98); DeKalb SSTX RIB = DeKalb brand Smartstax RIB (DKC 50-82); Pioneer AMX = Pioneer AcreMax Xtra (P0157AMX); Pioneer AMXT = Pioneer AcreMax Xtreme (P0339AMXT) 3 Insecticide listed as ounces a.i. per 1,000 row-feet 4 Furrow-SB = insecticide applied with SmartBox system at planting time; Furrow = insecticide applied at planting time 5 Chemical and check means based on 20 observations (5 roots/2 rows x 4 replications) 6 Iowa State Node-Injury Scale (0-3). Number of full or partial nodes completely eaten 7 Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05) 8 Product consistency = Percentage of times nodal injury was 0.25 (¼ node eaten) or less

23

Table 18. Average Stand Count for Insecticide on Bt Corn: Northeast Iowa Research and Demonstration Farm, Nashua1

Stand


DeKalb non-RW Bt + Index 2.80CS 0.27 Furrow 33.00a DeKalb non-RW Bt ----- ----- ----- 32.75a DeKalb SSTX RIB + Index 2.80CS 0.27 Furrow 32.50ab Pioneer AMX ----- ----- ----- 32.50ab Pioneer AMX + Aztec-HC 9.34G 0.14 Furrow-SB 32.50ab DeKalb SSTX RIB ----- ----- ----- 32.25ab DeKalb non-RW Bt + Aztec-HC 9.34G 0.14 Furrow-SB 31.75abc DeKalb SSTX RIB + Aztec-HC 9.34G 0.14 Furrow-SB 31.75abc Pioneer AMX + Index 2.80CS 0.27 Furrow 31.75abc Pioneer AMXT + Index 2.80CS 0.27 Furrow 29.75 bc Pioneer AMXT + Aztec-HC 9.34G 0.14 Furrow-SB 29.50 cd Pioneer AMXT ----- ----- ----- 27.50 d


2 non-RW Bt = an absence of any Bt trait targeting corn rootworm; DeKalb non-RW Bt = DeKalb brand VT2P (DKC 62-98); DeKalb SSTX RIB = DeKalb brand Smartstax RIB (DKC 50-82); Pioneer AMX = Pioneer AcreMax Xtra (P0157AMX); Pioneer AMXT = Pioneer AcreMax Xtreme (P0339AMXT) 3 Insecticide listed as ounces a.i. per 1,000 row-feet 4 Furrow-SB = Insecticide applied with SmartBox system at planting time; Furrow = insecticide applied at planting time 5 Chemical and check means based on 16 observations (2 rows/ treatment x 17.4 row-ft/treatment x 4 replications x 2 evaluation dates) 6 Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05) Table 19. Average Yield for Insecticide on Bt Corn: Northeast Iowa Research and Demonstration Farm, Nashua1

Bushels/


DeKalb non-RW Bt + Index 2.80CS 0.27 Furrow 249a DeKalb non-RW Bt ----- ----- ----- 242ab DeKalb non-RW Bt + Aztec-HC 9.34G 0.14 Furrow-SB 228abc DeKalb SSTX RIB + Aztec-HC 9.34G 0.14 Furrow-SB 222abc DeKalb SSTX RIB + Index 2.80CS 0.27 Furrow 222abc Pioneer AMXT + Aztec-HC 9.34G 0.14 Furrow-SB 220abc Pioneer AMXT ----- ----- ----- 217abc Pioneer AMX + Index 2.80CS 0.27 Furrow 212abc DeKalb SSTX RIB ----- ----- ----- 211abc Pioneer AMX ----- ----- ----- 210abc Pioneer AMX + Aztec-HC 9.34G 0.14 Furrow-SB 206 bc Pioneer AMXT + Index 2.80CS 0.27 Furrow 203 c

1 Planted May 4, 2017; machine harvested October 25, 2017 2 non-RW Bt = an absence of any Bt trait targeting corn rootworm; DeKalb non-RW Bt = DeKalb brand VT2P (DKC 62-98); DeKalb SSTX RIB = DeKalb brand Smartstax RIB (DKC 50-82); Pioneer AMX = Pioneer AcreMax Xtra (P0157AMX); Pioneer AMXT = Pioneer AcreMax Xtreme (P0339AMXT) 3 Insecticide listed as ounces a.i. per 1,000 row-feet 4 Furrow-SB = insecticide applied with SmartBox system at planting time; Furrow = insecticide applied at planting time 5 Chemical and check means based on 4 observations (2-row treatment x 68 row-feet/treatment x 4 replications) 6 Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05) 7 Yields converted to 15.5% Moisture

24

Table 20. Average Root Injury and Product Consistency for Insecticide on Non-Bt Corn: Northeast Iowa Research and

Demonstration Farm, Nashua1

Node- Product


Pioneer non-RW Bt + MBI-305BM1SE1 ----- 0.5 Furrow 0.46 50 Pioneer non-RW Bt + Venerate XC ----- 1.0 Furrow 0.53 55 Pioneer non-RW Bt + Venerate XC ----- 0.75 Furrow 0.66 45 Pioneer non-RW Bt + Capture LFR 1.5SC 0.10 Furrow 0.66 55 Pioneer non-RW Bt + MBI-305BM1SE1 ----- 1.0 Furrow 0.70 40 Pioneer non-RW Bt + Venerate XC ----- 0.5 Furrow 0.74 15 Pioneer non-RW Bt + MBI-305BM1SE1 ----- 0.75 Furrow 0.77 20 Pioneer non-RW Bt ----- ----- ----- 0.83 20

1 Planted May 4, 2017; evaluated August 2, 2017

2 non-RW Bt = an absence of any Bt trait targeting corn rootworm; Pioneer non-RW Bt = Pioneer brand RR (P0448HR) 3 Capture LFR Insecticide listed as ounces a.i. per 1,000 row-feet. Venerate XC & MBI-305BM1SE1 products listed as gallon per Acre

4 Furrow = insecticide applied at planting time 5 Chemical and check means based on 30 observations (5 roots/2 rows x 6 replications) 6 Iowa State Node-Injury Scale (0-3). Number of full or partial nodes completely eaten 7 No significant differences between means (ANOVA, P < 0.05) 8 Product consistency = Percentage of times nodal injury was 0.25 (¼ node eaten) or less 9 No significant differences between means (ANOVA, P < 0.05) Table 21. Average Stand Count for Insecticide on Non-Bt corn: Northeast Iowa Research and Demonstration Farm,

Nashua1

Stand


Pioneer non-RW Bt + MBI-305BM1SE1 ----- 0.75 Furrow 30.50 Pioneer non-RW Bt ----- ----- ----- 30.50 Pioneer non-RW Bt + MBI-305BM1SE1 ----- 1.0 Furrow 30.00 Pioneer non-RW Bt + Venerate XC ----- 0.5 Furrow 29.50 Pioneer non-RW Bt + Capture LFR 1.5SC 0.10 Furrow 29.50 Pioneer non-RW Bt + Venerate XC ----- 1.0 Furrow 29.50 Pioneer non-RW Bt + MBI-305BM1SE1 ----- 0.5 Furrow 29.00 Pioneer non-RW Bt + Venerate XC ----- 0.75 Furrow 28.00


2 non-RW Bt = an absence of any Bt trait targeting corn rootworm; Pioneer non-RW Bt = Pioneer brand RR (P0448HR) 3 Capture LFR Insecticide listed as ounces a.i. per 1,000 row-feet. Venerate XC and MBI-305BM1SE1 products listed as gallon per Acre

4 Furrow = Insecticide applied at planting time

5 Chemical and check means based on 24 observations (2 rows/ treatment x 17.4 row-ft/treatment x 6 replications x 2 evaluation dates) 6 No significant differences between means (ANOVA, P < 0.05)

25

Table 22. Average Yield for Insecticide on Non-Bt corn: Northeast Iowa Research and Demonstration Farm, Nashua1

Bushels/


Pioneer non-RW Bt + MBI-305BM1SE1 ----- 0.75 Furrow 268 Pioneer non-RW Bt ----- ----- ----- 256 Pioneer non-RW Bt + MBI-305BM1SE1 ----- 1.0 Furrow 254 Pioneer non-RW Bt + Capture LFR 1.5SC 0.10 Furrow 246 Pioneer non-RW Bt + MBI-305BM1SE1 ----- 0.5 Furrow 242 Pioneer non-RW Bt + Venerate XC ----- 0.5 Furrow 238 Pioneer non-RW Bt + Venerate XC ----- 1.0 Furrow 235 Pioneer non-RW Bt + Venerate XC ----- 0.75 Furrow 231

1 Planted May 4, 2017; machine harvested October 25, 2017 2 non-RW Bt = an absence of any Bt trait targeting corn rootworm; Pioneer non-RW Bt = Pioneer brand RR (P0448HR) 3 Capture LFR Insecticide listed as ounces a.i. per 1,000 row-feet. Venerate XC & MBI-305BM1SE1 products listed as gallon per Acre

4 Furrow = insecticide applied at planting time 5 Chemical and check means based on 6 observations (4-row treatment x 19 row-feet/treatment x 6 replications) 6 No significant differences between means (ANOVA, P < 0.05) 7 Yields converted to 15.5% Moisture

26

APPENDIX I

Agronomic Information

27

2017 Field History Data

Ames, IA-ISU Johnson Farm Boone, IA-ISU Bruner Farm

Corn Rootworm studies Corn Rootworm studies (following trap crop) (following trap crop)

Insecticide History

2016 No Insecticide (trap crop) Corn (Trap crop)

2015 Insecticide Test Plot Corn (Insecticide Test Plot)

2014 No Insecticide (trap crop) Corn 2013 Insecticide Test Plot Corn

Tillage Fall 2016-disk ripper Spring 2017-field cultivation (2X)

Fall 2016-chopped stalks & Chisel plow; Spring 2017-field cultivation (2X)

Variety DeKalb DKC 50-82 SSTX RIB, DeKalb DKC 62-98 VT2P RIB,

Pioneer P0157AMX, Pioneer P0339AMXT

DeKalb DKC62-98 RIB VT2P

Planting Date

May 15

May 15

Planting Rate 35,600 seeds/A 35,600 seeds/A

Herbicides1

2 pints/acre Surestart II + 10 oz Harness-April 27-PRE; 3 oz/acre Capreno-June 9-POST

2.75 qts. Acuron-April 26-PRE; 3 oz. Laudis-June 13-POST; 1 oz. Impact-June 30-POST

Fertilizer2

N P K S Zn

N P K S Zn

Fall/2016 Preplant

19 64 96 16 1.6 200 --- --- --- ---

24 80 120 20 2 194 --- --- --- ---

Dates Stand Count June 5, October 12 June 5, October 12

Root Digging July 20, 28, August 9, 15 August 11

Lodging October 12 October 12

Harvest

October 23 October 26

Soil Type Clay loam & loam Silty clay loam

Soil Organic Matter % 3.4 3.5

Soil pH 5.7 6.1 1 Expressed as formulation per acre. 2 Expressed as pounds per acre. Spring-preplant 200 lbs. actual N in 32% UAN form applied on April 27 at ISU Johnson Farm (Ames); Fall/2016 applied- 19 lbs. Nitrogen, 64 lbs. Phosphorus, 16 lbs. Potassium, 96 lbs. Sulfur, 16 Sulfur and 1.6 Zinc applied on November, 2016 at ISU Johnson Farm (Ames); Fall/2016 applied- 24 lbs. Nitrogen, 80 lbs. Phosphorus, 120 lbs. Potassium, 20 lbs. Sulfur, 2 lbs. Zinc applied on November, 2016 ISU Bruner Farm (Boone); Spring- Spring-preplant 194 lbs., UAN applied on April 26 at ISU Bruner Farm (Boone).

28

2017 Field History Data

1 Expressed as formulation per acre. 2 Expressed as pounds per acre. Spring/2017- 200 lbs. of Potassium along with 3 Tons/acre Ag. Lime applied on March 10. Spring-Preplant 220 lbs. per acre actual nitrogen applied as anhydrous ammonia (82.5-0- 0) on March 23 at Crawfordsville. Spring-Preplant 200 lbs. per acre actual nitrogen applied as anhydrous ammonia (82.5-0- 0) on April 9 at Nashua.

Crawfordsville, IA-ISU Southeast Farm

Nashua, IA-ISU Northeast Farm

Corn Rootworm studies Corn Rootworm studies (following trap crop) (following trap crop)

Insecticide History 2016 No Insecticide (trap crop) No Insecticide (trap crop) 2015 Insecticide Test Plot Insecticide Test Plot 2014 No Insecticide (trap crop) No Insecticide (trap crop) 2013 Insecticide Test Plot Insecticide Test Plot Tillage

Fall 2016-chisel plow; Spring 2017-field cultivation

Fall 2016-chisel plow; Spring 2017-field cultivation

Variety DeKalb DKC 62-98 VT2P RIB,

Munson 6824RR.

DeKalb DKC 50-82 SSTX RIB, DeKalb DKC 62-98 VT2P RIB,

Pioneer P0157AMX, Pioneer P0339AMXT, Pioneer P0448HR.

Planting Date(s)

May 9

May 4

Planting Rate 35,600 seeds/A 35,600 seeds/A

Herbicides1

2 oz. Zidua + 1.5 lbs. Atrazine + 32 oz. Roundup PowerMax + 2-lbs. AMS-May 18-PRE; 4 pt. Halex GT + 0.5 lbs. Atrazine + 2 lbs. AMS + 12.8 oz.–June 12-POST;

2.3 qts. Harness Extra-May 10-PRE; 32 oz. Roundup WeatherMax-June 9-POST;

Fertilizer2

N P K Lime

N P K

Spring/2017 Preplant

--- --- 200 3 T/A 220 --- --- ---

--- --- --- 200 --- ---

Dates

Stand Count June 2, October 24 May 31, October 13 Root Digging July 24, August 7 July 17 & 28, August 1 Lodging October 24 October 13 Harvest

October 24 October 25

Soil Type Silty Clay Loam Loam

Soil Organic Matter % 5.1 4.5 Soil pH 4.9 6.9

29

APPENDIX II

Weather Data

30

Ames & Boone1

2017 Rainfall and Temperature April May June

Temp (F) Rainfall Temp (F) Rainfall Temp (F) Rainfall

Day Low High Inches Low High Inches Low High Inches

1 35 55 40 44 0.56 51 83 2 44 50 0.02 34 63 61 89 3 46 50 0.64 39 60 64 91 4 45 57 42 68 67 92 5 41 53 0.01 44 77 65 86 6 34 54 50 72 60 83 7 32 61 45 80 56 83 8 42 75 54 84 60 87 9 56 78 57 81 61 90

10 38 57 54 69 1.09 69 93 11 37 60 50 70 74 94 12 39 68 0.16 47 77 73 89 13 50 71 0.01 51 82 70 92 0.34 14 51 68 0.33 56 86 64 85 0.65 15 57 72 0.18 63 91 65 91 16 49 69 61 90 0.88 65 87 0.25 17 45 74 57 77 0.35 63 83 0.02 18 53 67 0.11 50 62 0.18 56 77 19 46 72 0.40 45 50 1.02 56 82 0.01 20 43 54 45 63 0.30 58 89 21 39 58 43 55 60 90 22 41 66 46 73 0.09 68 86 1.02 23 40 74 49 59 0.28 58 74 24 44 74 50 62 0.24 52 77 25 47 70 0.01 47 72 50 73 26 36 48 57 82 53 73 0.09 27 32 49 55 70 0.22 52 77 28 31 43 0.37 54 80 65 84 0.37 29 38 43 0.16 52 75 63 83 0.06 30 38 43 1.32 49 75 61 80 0.02 31 46 77

Mean/Total 51.8 3.72 60.6 5.21 73.0 2.83

Normal 50.8 3.72 61.6 4.81 70.7 4.96

D.F.N. 1.0 0.00 -1.0 0.40 2.30 -2.13 1. Weather station located 5 miles WSW of Ames at ISU AEA/Agronomy Farm. Low/High Temperatures & Rainfall

amounts for Ames & Boone from Mesonet Weather Station website at: http://mesonet.agron.iastate.edu/agclimate/

http://mesonet.agron.iastate.edu/agclimate/

31

Ames & Boone1

2017 Rainfall and Temperature July August September



1 58 80 62 87 0.89 53 74 2 61 85 63 87 59 83 3 63 90 52 76 0.44 56 85 4 67 89 50 78 53 73 5 64 90 53 75 48 70 6 69 91 58 77 44 70 7 67 85 62 79 45 77 8 63 87 57 82 51 83 9 65 91 55 82 54 82 10 66 86 61 83 60 83 11 65 91 57 78 53 84 12 69 89 0.85 55 83 57 86 13 64 83 60 78 0.02 57 90 14 59 82 60 84 0.27 59 92 15 61 91 60 72 0.11 65 93 16 71 89 65 82 0.07 60 80 0.58 17 68 89 61 79 52 74 0.15 18 73 88 59 84 0.14 55 71 0.15 19 71 94 60 82 61 82 20 70 97 0.63 67 90 0.26 65 87 21 75 85 0.03 64 80 1.08 61 89 22 72 87 56 75 70 92 23 62 83 51 80 0.01 69 92 24 59 80 60 76 66 91 25 65 90 61 76 0.01 63 86 0.79 26 70 80 0.17 60 80 0.02 55 68 0.03 27 66 84 61 81 49 70 28 64 85 58 71 47 79 29 61 82 56 81 51 74 30 59 82 57 84 46 72 31 59 84 59 78

Mean/Total 76.0 1.68 69.4 3.32 68.6 1.70 Normal 74.0 4.83 72.0 4.82 64.8 3.25 D.F.N. 2.0 -3.15 -2.6 -1.5 3.8 -1.55 1 Weather station located 5 miles WSW of Ames at ISU AEA/Agronomy Farm.

32

Ames & Boone1

2017 Rainfall and Temperature

October



1 51 73 0.01 2 57 83 0.44 3 58 80 0.27 4 52 71 0.01 5 50 63 0.20 6 58 68 1.73 7 53 66 1.20 8 48 82 9 43 66

10 40 46 0.72 11 42 61 12 48 63 13 48 58 0.23 14 48 63 0.67 15 39 57 16 36 70 17 47 75 18 46 76 19 46 75 20 52 75 21 51 72 0.08 22 42 66 23 41 63 24 37 52 25 35 66 26 36 64 27 31 38 28 28 45 29 23 49 0.08 30 32 45 31

Mean/Total 54.1 5.64 Normal 52.5 2.56 D.F.N. 1.6 3.08 1 Weather station located 5 miles WSW of Ames at ISU AEA/Agronomy Farm.

33

Crawfordsville1




1 32 58 43 53 0.45 49 84 2 45 54 41 61 0.10 59 89 3 48 57 0.15 37 57 65 92 4 48 55 0.22 38 69 68 94 5 40 48 0.65 40 74 59 91 6 35 54 0.40 48 71 53 82 7 30 60 40 72 52 81 8 39 78 48 79 55 86 9 57 81 59 76 62 91

10 47 73 0.15 58 73 63 91 11 40 60 0.05 56 73 0.60 71 93 12 36 72 52 76 72 95 13 50 78 0.05 48 83 73 96 14 50 77 55 85 70 92 0.15 15 60 84 0.20 61 92 67 94 16 51 72 0.20 66 89 67 91 0.10 17 41 76 66 86 70 89 0.60 18 48 78 59 74 0.25 63 80 19 54 80 47 59 0.25 58 83 20 50 72 48 73 0.75 56 92 21 40 58 0.35 46 58 61 85 0.05 22 38 66 44 70 71 89 23 35 75 48 66 62 83 0.15 24 43 79 47 66 54 77 25 50 80 45 76 48 75 26 42 61 0.20 57 70 1.00 49 75 27 38 53 0.50 54 75 44 78 28 38 46 0.10 58 81 64 86 0.15 29 40 47 0.20 55 78 64 79 30 42 55 1.00 50 80 65 82 31 48 78

Mean/Total 54.9 4.42 61.9 3.40 73.8 1.20 Normal 51.4 4.17 63.2 5.35 72.1 7.43 D.F.N. 3.5 0.25 1.3 -1.95 1.7 -6.23 1 Weather station located at study site. Rainfall amounts manually recorded at study site. Low/High Temperatures

for Crawfordsville from Mesonet Weather Station website at: http://mesonet.agron.iastate.edu/agclimate/


34

Crawfordsville1




1 57 84 61 87 46 73 2 55 89 60 89 44 80 3 63 94 54 83 0.10 56 87 4 66 88 52 76 59 80 5 64 90 49 80 47 70 6 62 93 58 73 44 67 7 64 87 55 80 42 73 8 59 85 50 84 48 84 9 64 94 49 83 52 82

10 67 91 1.00 62 89 49 79 11 69 92 57 77 0.90 44 79 12 73 90 0.20 52 79 47 82 13 66 85 0.25 50 78 46 87 14 60 80 57 84 54 90 15 53 89 62 83 59 92 16 67 89 68 84 65 91 0.30 17 61 83 65 81 53 74 18 65 93 58 87 48 82 19 70 94 1.00 62 84 0.10 58 84 0.10 20 73 97 63 83 68 95 21 72 93 0.35 65 84 0.55 67 89 2.10 22 70 90 55 76 0.40 70 94 0.50 23 64 88 0.15 51 78 65 96 24 61 80 54 80 65 93 25 60 85 58 74 62 94 26 70 88 56 78 60 78 27 66 86 0.30 61 75 0.10 47 71 28 59 82 58 78 42 78 29 55 80 54 79 45 77 30 54 83 53 83 40 74 31 55 83 53 76

Mean/Total 75.6 3.25 68.8 2.15 67.8 3.00 Normal 74.4 3.47 73.1 4.90 65.7 3.50 D.F.N. 1.2 -0.22 -4.3 -2.75 2.1 -0.50 1Weather station located at test site. Rainfall amounts manually recorded at study site.

35

Crawfordsville1


October



1 50 78 2 64 89 3 65 85 4 57 70 0.50 5 53 71 6 67 72 7 52 72 0.55 8 51 82 9 49 79

10 50 58 11 48 56 0.80 12 54 62 13 54 79 14 60 70 15 38 62 2.20 16 35 68 17 44 74 18 48 76 19 43 78 20 50 78 21 60 83 22 45 61 0.90 23 43 62 24 39 52 25 36 64 26 40 71 27 34 40 28 23 39 29 22 52 30 35 48 31

Mean/Total 57.3 4.95 Normal 53.7 2.89 D.F.N. 3.6 2.06 1 Weather station located at test site. Rainfall amounts manually recorded at study site.

36

Nashua1




1 31 61 38 45 0.45 46 82 2 43 50 0.07 37 58 56 89 3 45 48 1.14 32 63 67 94 4 42 56 34 68 62 90 5 40 50 37 75 59 84 6 30 52 41 69 53 81 7 25 58 41 72 51 83 8 39 74 48 76 0.09 60 88 0.03 9 53 78 47 74 0.03 59 90

10 39 64 50 60 0.38 67 92 11 35 54 44 69 73 96 12 35 67 0.59 42 74 68 89 0.04 13 43 60 50 85 67 94 0.38 14 48 62 0.42 55 85 65 84 0.15 15 56 73 0.14 49 85 1.26 62 90 0.01 16 44 67 62 86 0.35 64 89 0.80 17 45 68 57 79 0.53 64 80 0.03 18 46 65 0.02 46 60 59 75 19 44 61 0.96 40 48 0.56 55 78 Tr-rain 20 43 57 42 55 0.33 51 81 21 34 59 42 51 tr-rain 55 75 0.13 22 36 66 41 70 0.57 67 81 1.79 23 37 73 49 58 0.20 57 70 Tr-rain 24 44 74 47 62 50 71 0.05 25 51 69 0.02 43 71 tr-rain 47 69 26 37 55 tr-rain 54 73 tr-rain 49 70 27 31 40 tr-sleet 51 73 0.03 44 77 28 25 45 0.11 53 77 62 74 1.33 29 35 46 0.03 51 71 58 78 0.35 30 39 43 0.81 47 72 56 79 0.06 31 46 77

Mean/Total 49.9 4.31 57.5 4.79 70.4 5.15 Normal 47.7 3.79 59.5 4.41 68.9 5.63

D.F.N. 2.2 0.52 -2.0 0.39 1.5 -0.48 1 Weather station located at test site. Rainfall amounts manually recorded at study site. Low/High Temperatures for Nashua from Mesonet Weather Station website at: http://mesonet.agron.iastate.edu/agclimate/


37

Nashua1




1 58 75 55 85 43 71 2 57 84 58 85 53 79 0.04 3 62 83 50 77 0.32 48 84 4 64 85 49 75 49 74 0.01 5 66 89 48 77 46 67 6 66 90 58 76 43 65 7 62 83 52 77 37 76 8 56 82 48 84 43 77 9 54 87 53 81 tr-rain 51 79

10 65 84 1.28 59 81 tr-rain 54 80 11 64 81 tr-rain 53 76 49 83 12 67 92 0.93 51 78 51 86 13 58 79 48 71 tr-rain 52 89 14 53 75 59 82 0.50 57 90 15 57 89 58 71 0.01 62 91 tr-rain 16 66 83 64 82 0.21 60 84 0.07 17 61 84 61 77 50 71 0.70 18 66 88 0.04 56 77 0.01 51 67 0.17 19 65 88 0.72 52 83 53 81 20 72 86 61 87 58 88 21 67 73 2.50 62 78 0.56 57 92 22 69 85 2.73 51 74 73 93 23 60 80 47 77 66 93 24 56 76 54 73 tr-rain 64 92 25 61 83 57 74 0.05 60 84 1.26 26 69 79 0.15 57 73 53 68 27 62 82 59 79 44 69 28 57 84 54 70 41 79 29 55 83 52 75 41 67 30 56 84 51 85 40 70 31 58 85 52 73 0.09

Mean/Total 72.3 8.35 66.2 1.75 65.7 2.25 Normal 72.0 4.78 69.6 4.51 61.9 3.00 D.F.N. 0.3 3.57 -3.4 -2.76 3.8 -0.75 1 Weather station located at Study site. Rainfall amounts manually recorded at study site.

38

Nashua1


October



1 52 73 2 60 80 0.27 3 54 80 0.11 4 44 74 5 44 68 0.03 6 55 67 1.48 7 50 68 1.43 8 47 81 9 43 66

10 42 52 0.50 11 44 54 tr-rain 12 52 61 tr-rain 13 50 61 tr-rain 14 49 62 0.26 15 38 57 16 31 68 0.01 17 42 74 18 46 77 19 36 72 20 48 76 21 52 71 0.67 22 43 65 23 40 62 tr-rain 24 35 52 25 32 61 26 34 62 27 32 36 0.04 28 19 37 29 20 46 0.07 30 28 43 31

Mean/Total 52.8 4.86 Normal 49.3 2.40 D.F.N. 3.5 2.46 1 Weather station located at test site. Rainfall amounts manually recorded at study site.

39

APPENDIX III

Materials Tested

40

Table 1. Conventional insecticides used in studies in 2017

Product name

Rate

(ounces a.i. per 1000

row-feet; unless

specified) Active ingredient Company

Aztec-HC 9.34G 0.14 tebupirimphos & cyfluthrin

AMVAC Chem.

Corp.

Capture LFR 1.5SC 0.10 bifenthrin FMC Corporation

Capture 3RIVE 3D

1.5SC 0.10 bifenthrin FMC Corporation

Ethos XB 0.10 bifenthrin FMC Corporation

Force 250CS 0.12 & 0.14 tefluthrin Syngenta

Force HL 10G 0.14

tefluthrin

AMVAC Chem.

Corp.

Index 2.80CS 0.27 bifenthrin

AMVAC Chem

Corp.

Puma 4 oz./A Growth regulator FMC Corporation

Smartchoice-HC 15G 0.14

Chlorethoxyfos & bifenthrin

AMVAC Chem

Corp.

Venerate XC

0.5, 0.75 & 1.0

Gallon/Acre

Heat-killed Burkholderia spp. strain A396

cells and spent fermentation media

Marrone Bio

Innovations

41

Table 2. Rootworm active Bt Traits

Product name

Events targeting

rootworm Toxin Company

Herculex DAS-59122-7 Cry34/35Ab1

Pioneer & Dow

AgroSciences

Pioneer AMX DAS-59122-7 Cry34/35Ab1 DuPont-Pioneer

Pioneer AMXT

DAS-59122-7 +

MIR604 Cry34/35Ab1 + mCry3A DuPont-Pioneer

Pioneer OAM1 DAS-59122-7 Cry34/35Ab1 DuPont-Pioneer

Smartstax RIB

DAS-59122-7 +

MON 88017 Cry34/35Ab1 + Cry3Bb1

Dow AgroSciences

& Monsanto

42

APPENDIX IV

2017 Research Photos

Trimming plants

Early season sampling of plants for lab analysis

2017 iowa evaluation of insecticides and plant ... · plant-incorporated protectants corn pests...

Documents