2016 point of care in-office lab testing · 2016. 9. 19. · •all entities who perform even 1 lab...
TRANSCRIPT
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2016 Point of Care In-Office
Lab Testing
Judy Perrigin MT(ASCP), OD
UHCO
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Point of CareTesting (POCT)
• Bedside / chairside / EMS
• Rapid, immediate results
• If CLIA waived (still need CLIA Certificate)
– Easy, almost fool proof
– Some are devices / kits FDA approved for
home use by consumers as well as for
professional use
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POCT Examples
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Requirements
• Must meet all OSHA, CDC, & CLIA
requirements as previously described
• Also lab coat recommended when dealing
with blood or other body fluids, tissues
– Do not wash in home laundry
– Do not wear outside the workplace
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• CLIA categorizes laboratory tests
according to complexity & states staff
qualifications needed for each
– Waived
– Moderate / PPM
– Complex
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Who needs a CLIA Certificate?
• All entities who perform even 1 lab test,
including waived tests, on materials from
the human body for purpose of dx,
prevention, or treatment of any condition
must register with CLIA
– Required even if not charging for it
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Waived & PPM
– Must follow good clinical laboratory practices
• Follow manufacturer’s recommendations
• Run and record control values in log and in
patient’s chart
• Maintain a log of patient and control values
• Maintain record of action taken: referral, etc
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Home collection kits
• Not technically POCT
• Do not require CLIA certificate because you are just collecting not performing the test
• Specimen collected at home or in-office and mailed or transported to medical lab
• Macula Risk PGx, RetnaGene, fluorescent antibody slides preps, etc.
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OD most likely to use
• Blood glucose screening
• Hb A1C (glycosylated hemoglobin)
• RPS for Adenovirus & Inflammadry for dry
eye associated with increased MMP-9
• Cholesterol & HDL
• Tear Lab & newer dry eye tests
– Some use tears, some use blood
• Conjunctival smears (must have PPM
level of certificate)
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CPT CODES
• A1C Now if performed in provider’s office
CPT 83037
• For Medicare or Medicaid 83037QW
• Finger puncture or other capillary blood
CPT 364.16
• Venipuncture blood collection CPT 364.15
• You do not have to memorize these
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Hgb A1C (glycosylated/glycated
hgb)
• Glucose attaches to a hgb molecule/glycates
• Irreversible for life of the RBC (2-3 months)
• A1C has greater affinity for O2
– Less O2 released to tissues so tissue damage
• Normal non-diabetics: 4-6%
• In known diabetics values of 7% or less often considered good control
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A1C
• Used to monitor long term control and
tx compliance & for dx
– Reflects blood glucose over past few
months
• Perform no more than every 3-4 months
• Not valid if hgb abnormalities such as
sickle cell, hemolytic anemias, etc
• Home self-test which can be used in-office
& laboratory procedure also available
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A1C
• Fasting not required
• Shows what average of blood glucose was
over past 2-3 months
• Per ADA now recommended for
diagnosis as well as monitoring DM
patients
• DX value: > 6.5%
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Relationship of A1C to Average Whole
Blood and Plasma Glucose Levels
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A1C studies
• In general lowering A1C by 1% decreases
risk of DM complications by 21%
• For eye, kidney, lowers risk of
complications by 40%
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DCCT A1C levels and the risk of
complications in type 1 diabetes
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A1CNOW +
• For professional use
• Manufacturer Metrika / Bayer
www.A1cnow.com
– Available from various distributors
– 10 & 20 cartridge packs/tests
– Same thing OTC in stores for patients in
packs of 2
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Bayer A1CNow+
• www.A1cnow.com
• 5 ul blood, 5 minutes
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• CPT*: 83037QW
• $21.06 average reimbursement
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Supplies needed
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Getting ready
• All parts of kit must be same lot # & at room temp for at least 1 hour
• Avoid performing in direct sun, on cold or warm surfaces
• Control should be run
• If you make mistakes at any point, test will not measure but you won’t know it until after specimen is applied to pad so reagents will be wasted
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Wash & dry hands, clean selected
finger with alcohol
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Dry with clean cotton or gauze
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Cap indicates new unused lancet
Only use devices which self-retract
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Choose fleshy non-callused finger
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Milk if needed
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• Good sample Not enough blood & too spread out
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Aspirate blood
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Fill completely & no bubbles
• Over or under filling will alter results
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Fully insert blood collector into
sampler body / diluent
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Shake/mix vigorously 6-8 times
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Stand sampler on table while
preparing cartridge
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Insert cartridge into monitor
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Don’t do this until after collecting
blood
Blood must be applied within 2 minutes of inserting cartridge
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Remove base of sampler & apply
diluted blood to pad
• 5 minutes later reading will appear in
window
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Error reading
Will not read if not performed properly
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Lipid profile
Assay of LDL, HDL, total cholesterol, Chol/HDL, triglycerides along with CHD risk factor
• Total cholesterol does not require fast, but the other lipid profile tests require 12 hr fast
• ADA goals for diabetics: triglycerides <150mg/dl, HDL >40mg/dl, LDL decrease by 40% from baseline
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Why order lipid panel?
• Chronic heart disease is the most common
cause of death in diabetics
• Low HDL &/or high LDL associated with
atherosclerosis & > incidence of
retinopathy
• Improving lipid profile may decrease
macular exudates and improve acuity in
diabetes, HBP, ARMD
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CardioChek PA Analyzer Test
• CLIA waived, FDA cleared for
professional use, finger stick, results in 1-2
minutes
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Thyroid (TSH) Hormone Testing
Kits
FDA Cleared and CLIA waived
Results in 10 minutes
CPT code: 84443, Reimbursement $23.40.
Kit cost approx $140- $160 for 20 tests
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Adeno Detector
• Currently only available for adenovirus
• Plan for more organisms in future
• Why not used more often? AV typically
easy to dx from clinical px, sxs
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Rapid Pathogen Screening (RPS)
AdenoPlus
• Detects all adenovirus subtypes
• In-office immunodiagnostic 10 minute test
– Uses antigen detection
• Highly specific (96%) & sensitive (90%)
• Assists in patient management re
contagion, need for antibiotics, etc
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Procedure
• 1. Assemble the detector
• 2. Dab sampling pad inside lower palp
conj 4-6 X . Let pad rest against conj for
an additional 3 sec to ensure saturation
• 3. Immerse the assembled detector tip
into the buffer vial for 15 sec
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Results
• If Results line (red or pink) & Control line
(blue) are both present, indicates
adenovirus antigen is present
Positive even if pink/redline is faint or
uneven
Negative: only 1 line
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Tear Lab
for osmolarity
• www.TearLab.com
• Use to:
– Dx presence of dry eye disease (DED)
– Grade severity of DED
– Monitor response to treatment
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Tear Lab & CLIA
• CPT code 83861 QW
• Reimbursement approx $46.80 per
patient
• Controls must be run which increases
your cost
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Tear hyperosmolarity
• Global biomarker for both aqueous
deficiency & evaporative dry eye
• The primary mechanism causing ocular
surface inflammation, damage, sxs
• Leads to cell death, loss of goblets, &
disturbance of mucin
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Rapid, noninvasive
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Results
• > 308 mOsms/L are indicative of DED
Amer Acad Ophthalmology Nov 2009.
Clinical trial conducted by Tear Lab
• Others use 316mOsms/L as cutoff
• >350 considered severe
• Dx values evolving as test is used more
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What is significant?
• High values
• Variability between the eyes
• Difference between OD and OS of
greater than 8-10 mOsm/L is a classic
indication of loss of homeostatic
control and is a confirming indicator of
dry eye disease.
• Variability from day to day & during day
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Point of Care Tests
• Many more on the horizon
• Becoming more commonplace in
Optometric offices
– Particularly in DM testing, dry eye
• Most CLIA waived
• Immediate results
• Most reimbursable
• It’s not that hard!