2014 bgri barbier presentation

#bgri2014

A consensus map for Ug99 stem rust resistance loci in wheat

Long-Xi Yu, Hugues Barbier, Matthew N. Rouse, Sukhwinder Singh, Ravi P. Singh, Sridhar Bhavani, Julio Huerta-Espino, and Mark E. Sorrells

Puccinia graminis: A global disease

Wheat Rust Pathogen Climate Yield Loss

Leaf Rust Puccinia recondita Mid temp. up to 20%

Stripe Rust Puccinia striiformis Low Temp. up to 90%

Stem Rust Puccinia graminis Warm Temp. up to 70%

Leaf Rust Stripe Rust Stem Rust


Winter host Summer host

From USDA

Puccinia graminis f. sp. tri-ci cycle


Toolbox of 65 resistance known genes/alleles

Sr related virulence Spreading of Ug99 from 1998 to 2010

Ug99: The game changer

1998/99 2000/01

2003/04

2004 2006

2007/8

2008

2009 2009/10

2010

Sr 31

Sr 24

Sr 36

Sr 21

Sr28

Sr13 Sr22

Sr40

Sr39

Sr33 Sr45

Sr35

Sr2

Sr21

Sr25

Sr26 Sr27

Sr29

Sr37

Sr42 Sr32*

Sr1RSAmigo

Sr50 Sr44

Sr43

Sr58

SrWeb Sr47

Sr51

Sr12

SrND643

Sr55

Sr56

Sr53

Sr52

Sr57 Sr46

SrTA1662

SrTA10187 SrTA10171

Sr31

Sr36

Sr32*

Sr32*

Sr30

SrCad

1A 2A 3A 4A 5A 6A 7A

1B 2B 3B 4B 5B 6B 7B

1D 2D 3D 4D 5D 6D 7D

Ug99 Resistance Loci

Sr2

Sr55

Sr56

Sr57

*Derived from MaHhew Rouse

Toward a consensus map for Ug99 Sr Loci

This study include:

- 3 studies on durum wheat (Tetraploide) - 18 studies on bread wheat (Hexaploide)

- 24 bi-parental mapping populations (30 parents) - 4 Association panels (LD-SRRSN/Spring & winter; ESWYT, AM durum panel) - 2 BC2 populations (2 parents)


0"

10"

20"

30"

40"

50"

60"

70"

80"

90"

100"

B"genome" A"genome" D"genome"

0"

5"

10"

15"

20"

25"

3B" 2B" 5B" 6B" 7D" 7B" 7A" 6A" 1B" 1A" 4A" 4B" 2A" 3A" 5A" 1D" 5D" 6D" 2D" 3D" 4D"

Chromosome arms

QTL num

ber

# Markers Size (cM) Average dis.

1A 71 161.5 2.3

1B 94 177.6 1.9

1D 42 135.5 3.2

2A 51 144 2.8

2B 128 178.2 1.4

2D 28 114.8 4.1

3A 54 174.5 3.2

3B 158 182.2 1.2

3D 48 139.5 2.9

4A 85 113.4 1.3

4B 44 108.3 2.5

4D 36 83.4 2.3

5A 64 196.5 3.1

5B 92 193.1 2.1

5D 16 121.3 7.6

6A 56 219 3.9

6B 83 128.1 1.5

6D 71 127.1 1.8

7A 77 203.3 2.6

7B 66 154.4 2.3

7D 69 122.3 1.8

Total 1433 3178 2.7

Chromosome 7

Chromosome 1

Chromosome 2

Chromosome 3

Chromosome 4

Chromosome 5

Chromosome 6


R-package: LPmerge

Linear Programming Algo.

Toward a consensus map for Ug99 Sr Loci BS00090594 wPt-5960gwm2570.0wPt-6627 wPt-68051.6BS000994694.6BS000950046.8BS000107349.7wPt-010018.1wPt-4916 wPt-6575wPt-0643 wPt-3459wPt-5934 wPt-519519.9wmc76421.3wmc38221.6wPt-3388 wPt-9230wPt-74402221.9wPt-537423.6SyOpL2770_NBS25.8SyOpL9828.2wPt-619928.8BS00110268 wmc243c28.9wPt-5556 wPt-7757wPt-5672 wPt-412529.1barc280a29.2wPt-8235 wPt-445329.6wPt-210630.4SyOpL174030.7wPt-570738.4

SNPPMOp1059844/1841.6SyOpL110043.7SyOpL39044.6wPt-9402 SNPPMOp150859/8wmc154

46.0

wPt-3561barc280bcfd238 wmc25

wPt-8398 wPt-8326wPt-8004 wPt-8404barc200 wPt-0462

53.8

wPt-9423 wPt-807254.2BS0006415654.4wPt-430154.6BS0006662654.9wPt-74363055.8wmc25756.0SyOpL266958.0BS0001068859.2wPt-260061.0wPt-858365.0SyOpL239266.2BS0002641266.3BS0007384270.6wPt-775071.1SyOpL16172.8SyOpL1333 SNPPMOp102229/3774.3wmc77075.2wPt-5878 SyOpL2163SyOpL1007 wmc474barc18

77.0wPt-033577.5wmc344 wPt-033578.0BS0000443378.6wPt-846079.6wPt-040880.0wPt-647780.4barc781.9barc1383.0BS0002209183.4wmc175A84.0BS00074789 gwm12086.8gwm14889.0gwm33890.0BS0000946192.0gwm50194.3wmc59294.8BS0000998996.3gwm38896.6wPt-3132 wPt-7200gpw5162 barc1042gwm120b97.1BS00110209 wPt-1964100.8wPt-4125102.0BS00003585102.4wmc175 wPt-0694104.0BS00022064 SNPPMOp1529541/32104.1wPt-0697105.8wmc332 wPt-3383108.2wPt-799851108.5wPt-5128111.9wPt-7004 wPt-7161115.4wPt-2108117.2wPt-2397121.7wPt-4892 barc1026130.6BS00009460131.2wPt-2274136.3

wmc602140.0

BS00100117144.0

wmc361148.2wmc317150.3

wPt-3436161.1

barc14b178.2

cfd56 SyOpL2571SNPPMOp149247/49 SNPPMOp29337/83wPt-10835 wPt-2960

0.0

cfd512.0barc903.0BS000720583.9

gwm45513.3BS0001049213.6gwm29617.0SNPPMOp113570/4317.7Ppd119.9BS00090678 cfd4421.1gwm261 wmc50321.4gpw29421.7gpw408025.8wmc11227.5

gwm48453.3

BS0001611656.8

wPt-424265.3gwm15766.0gwm53968.9

BS0001175287.0

wPt-1499114.8

2A 2B 2Dbarc124 wPt-733012wPt-1657 gwm356wPt-58870.0wPt-66872.4BS000227774.4wPt-77395.0barc1147a5.7

gwm38210.3

wmc45314.0gpw4474.214.2wmc52216.5

wPt-5647 wmc40722.9gwm339 gwm27524.6gwm9525.6gwm37226.9barc22027.8cfa226328.8barc115029.9wPt-995130.2wPt-636132.0BS00086365 wPt-409433.3wPt-311434.6

wPt-74065838.9SyOpL263439.3wPt-254443.7

barc31148.8SyOpL79751.3

SNPPMOp167763/46 gwm558wmc17761.9

wPt-4861 wPt-586572.4SyOpL141273.5wPt-705674.7

wPt-4201 BS00097263wPt-5251 wPt-7024wPt-701186.3gwm12086.8BS0002200290.9gwm29492.9gwm50194.3BS0000998996.3

BS00004405121.7

BS00100117144.0

Sr21

Sr39

Sr40

Sr36

Sr9aSrWeb

Sr47

Sr28

Sr32

Sr46

Sr32Sr32

CIM

MYT

- Unp

.

Kaur

et a

l. 20

09Bh

avan

i et

al.

2011

Bhav

ani

et a

l. 20

11Cros

sa e

t al.

2007

Cros

sa e

t al.

2007

Hie

bert

et a

l. 20

10Ro

use

et a

l. 20

12CI

MM

YT- U

np.

CIM

MYT

- Unp

.

Yu e

t al.

2011

Rous

e e

t al.

2014

Yu e

t al.

2011

A.M. B.P.A.M. B.P.

A.M. B.P.

gwm617_2 SNPPMOp1519638/550.0

wmc417_18.6

wmc57026.2

BS0001112545.0

PAPMM613738651.2wPt-1664 wPt-938251.9wPt-083254.4wPt-7475 wPt-137754.7BS0008363055.9wPt-66949860.4gwm334 BS00010933wPt-7623 wPt-6520

SNPPMOp10333/21wPt-4016

65.0

wPt-826678.2wPt-2573 wPt7127wPt-9690

86.0

gpw308787.7wPt-095988.7wPt-759989.1wPt-0357 wPt-8833wPt-3091

89.4

wmc807104.0

wPt-7373108.9

barc144112.9

BS00082104 gwm570119.3

wPt-5601125.3

wPt-8662133.1wPt-5310134.8

BS00021965140.9

wmc417149.0

gwm617158.0gwm427162.8wPt-6696164.9BS00003339167.0wPt-4229 wPt-9474167.3wPt-1661 wPt-1642wPt-9976 wPt-4445

167.6gpw7433.2169.0wPt-7204 wPt-1695wPt-8124

169.4

wPt-6268173.8

wmc254211.0

wmc59219.0

wPt-2175 wPt-7745wPt-8721 barc1136wmc397 gpw10790.0wPt-3774 wPt-7662

1.0 BS00003785 BS00003792BS000097951.4gwm881.5wPt-3376 wPt-49301.9barc11592.2barc10932.5 gwm582.8 gpw5076 barc3544.2 wPt-95325.2 wPt-7777 wPt-35816.2 wPt-6994 wPt-4720wPt-4283 wPt-7150wPt-5234 wPt-2689wPt-0245 wPt-1852wPt-3304

6.6

wPt-72076.9 wPt-82397.0 wPt-49247.2 wPt-1922 wPt-3130wPt-9990 wPt-4386wPt-80157.3wPt-30608.9wPt-46489.2BS0001044311.1 gwm17413.4wPt-559629.9BS00010415 cfd13wPt-533332.3BS0000385233.0BS00022668 wPt-3733gwm518 barc1169wPt-2964 wPt-7636

44.5

BS0008696450.9barc14a52.8wPt-546153.2wPt-4164 wPt-9952wPt-611656.5wPt-3045 wPt-126457.2wPt-826857.5BS0001099357.7wPt-503758.0wmc15258.7BS0010903658.9BS0002318760.6wPt-602361.1

wPt-680264.4gwm200a65.0

wmc41778.6BS0002248079.5

wPt-5480101.9BS00011458 BS00085589102.6wPt-0171106.8wPt-1541107.1BS00010618107.4wPt-9256110.1BS00023066110.4

BS00110651126.2wPt-732061128.1

BS00003785 BS00003792BS00009795 wPt-6994SNPPMM61159750/20 wPt-3774PAPMM6106536 wPt-7777wPt-7662 wPt-1922wPt-9532 wPt-4720wPt-4283 wPt-7207wPt-3130 wPt-1852wPt-9990 wPt-8239wPt-3304

0.0

SyOpL2811.0SyOpL22323.4SyOpL3624.6BS000104435.5SyOpL14577.3SyOpL1451 SyOpL191910.0wPt-3424 SNPPMOp1239709/6011.3SNPPMOp157697/3617.8wPt-217518.2SyOpL124718.5SyOpL1419.8wPt-559623.0BS00010415 cfd13SNPPMOp1227776/5026.6

wPt-533327.6BS0000385230.1

gwm518 SNPPMOp103652/2040.6SyOpL105141.2BS00022668 wPt-373341.5

BS0008696447.9wPt-546148.3SNPPMOp261873/8351.4wPt-503753.1BS0001099354.7BS0010903655.9BS0002318757.6wmc152 SNPPMOp3332865/80wmc417-2 SNPPMOp372691/2162.6wPt-680263.4SyOpL122573.4SyOpL74074.4SyOpL31375.0BS0002248076.5PAPMOp8618377.4wmc41777.6

BS00011458 BS0008558999.7wPt-5480100.9BS00010618104.5wPt-0171105.8wPt-1541106.1BS00023066107.4wPt-9256109.1

BS00110651123.2

wPt-732061127.1

6A 6B 6D

Sr26

Sr52

Sr13

Sr42 SrTA10187

Sr29

SrCad

Yu e

t al.

2011

Lett

a et

al.

2013

Cros

sa e

t al.

2007

CIM

MYT

- Unp

.

Yu e

t al.

2011

Bhav

ani e

t al.

2011

Bhav

ani e

t al.

2011

Cros

sa e

t al.

2007

Yu e

t al.

2011

CIM

MYT

- Unp

.CI

MM

YT- U

np.

Yu e

t al.

2011

Yu e

t al.

2011

Yu e

t al.

2011

Yu e

t al.

2011Cr

ossa

et a

l. 20

07Cr

ossa

et a

l. 20

07

A.M. B.P.A.M. B.P.A.M. B.P.

Sr28

Sr13 Sr22

Sr40

Sr39

Sr33 Sr45

Sr35

Sr2

Sr21

Sr25

Sr26 Sr27

Sr29

Sr37

Sr42 Sr32*

Sr1RSAmigo

Sr50 Sr44

Sr43

Sr58

SrWeb Sr47

Sr51

Sr12

SrND643

Sr55

Sr56

Sr53

Sr52

Sr57 Sr46

SrTA1662

SrTA10187 SrTA10171

Sr31

Sr36

Sr32*

Sr32*

Sr30

SrCad

1A 2A 3A 4A 5A 6A 7A

1B 2B 3B 4B 5B 6B 7B

1D 2D 3D 4D 5D 6D 7D



Merged QTL

Merged QTL

Merged QTL

Merged QTL

Merged QTL

Merged QTL

Merged QTL

Sr30


0"

5"

10"

15"

20"

25"

3B" 2B" 5B" 6B" 7D" 7B" 7A" 6A" 1B" 1A" 4A" 4B" 2A" 3A" 5A" 1D" 5D" 6D" 2D" 3D" 4D"

Chromosome arms

QTL num

ber

Toward a consensus map for Ug99 Sr Loci BS00003733 gwm389wmc50 barc276a0.0barc11771.0

gpw70804.1barc11116.1gwm6747.6

barc6711.9wPt-047612.6gpw422815.0

wPt-528118.7

BS0000062821.6wmc1122.5wmc42826.0wmc32227.9wPt-513328.6cfa207629.8wPt-2748 wPt-8855wPt-7341

31.9wPt-7217 barc31034.7wPt-304136.8barc29437.8barc32138.2wPt-864938.3wPt-9369 wPt-7992wPt-685440.5wmc11b42.9

gwm5 wPt-166567.5

cfd19370.5

SyOpL155677.7

wmc264 wPt-915483.5SyOpL108984.9

BS0005010995.0

BS00049978101.6wPt-9049102.2

wmc153 SyOpL2293109.5

SyOpL31116.6BS00024548117.2

wPt-4725128.7SyOpL424129.7

gwm480138.5

wPt-4065148.7wPt-732616149.0

BS00022092169.5BS00074926173.6BS00072145174.1wPt-3612174.5

BS000329120.0Sr27.7SNPPMOp3560048/58 SNPPMOp964081/710.1gwm38912.1wPt-844612.5SNPPMOp1089320/3 SNPPMOp1348570/5814.8wPt-8093 wPt-74233715.3wPt-604315.4BS00009440 BS00062676BS0006477816.0wPt-3921wPt-3761wPt-3609

16.9gwm533.1

17.1BS00009476BS0001253117.2 SNPPMOp10286/2817.9 BS0001153218.4 SyOpL205318.9 BS00011373

BS0001180619.0 wPt-66613920.1 SyOpL154222.6 wPt-1081

BS00022190gwm493 wPt-74264823.1SyOpL173024.7wPt-275725.2 cfd7926.1wPt-80021326.5wPt-721228.7BS0001196629.1SyOpL227330.7SyOpL239633.5wmc307 wmc23134.8SyOpL30537.0SyOpL136139.2

wPt-8238BS00070826BS0002204844.0cfd2846.1SyOpL230347.0BS0000951347.4wPt-680247.5BS0001006050.2SyOpL267550.4SyOpL105751.0wPt-37152.6gwm28554.1BS0001040555.3wmc43 SNPPMOp6664/5960.1BS00023074 BS0000386460.5wPt-7486 wPt-9510wmc78

barc1090barc290 wPt-623960.6

wPt-6973 wPt-115961.6wPt-420962.0SNPPMOp1295258/65 SNPPMOp1750525/83SNPPMOp400706/77 PAPMOp9653962.4gwm56662.6wPt-5105 BS00060014wPt-7015 wPt-9170wPt-6945

64.0

gpw745265.4SyOpL184565.7wPt-943265.8SyOpL193066.0barc111167.7wmc77768.1BS0011044568.2SyOpL75969.3BS00015099

wmc625

69.4barc1113 barc104470.4

wPt-6047 Sr12wPt-5580

wPt-594670.6

SyOpL858barc73wPt-5390gpw1120

71.1

wPt-075171.3BS0009427171.7BS0001013672.3wPt-943373.2wPt-571673.3

BS0007821473.6 BS0000970174.2

wPt-722974.3BS00023030 wPt-222074.7BS0000097875.3wPt-0544 wPt-044675.5SNPPMOp1095216/14

PAPMM6398764

wPt-3327 wPt-194076.7

wmc418 SyOpL2017wmc471 wPt-7502wPt-10003 wPt-408280.1BS0005941781.6BS0002240383.8BS0002224584.8SyOpL121785.1BS0010587888.2barc33491.2wPt-117192.2SyOpL261792.5BS0009737493.0wPt-991293.4wPt-805693.5wPt-576993.8wmc29197.2gwm10897.5wPt-5906 wPt-8096108.6wPt-3107110.2wPt-1804112.5SyOpL2491115.2SNPPMOp1526933/1115.5BS00070210116.7wPt-2439120.8barc77 SyOpL2194121.4gpw7258126.3SyOpL645133.0BS00021967141.6wPt-4412 gwm340143.2wPt-1311 wPt-4370143.5wPt-0365 gpw7220144.2wPt-0995 wPt-8352144.6gwm547182.2

BS00021930 wmc11SNPPMOp561314/18 wPt-39210.0

BS000004476.0gdm38 wmc11agdm99b13.3barc284 wPt-6909wPt-5313

14.9BS0001025815.6wmc4316.0wPt-9401 wPt-174116.3gdm136a16.4wPt-3815 wPt-133616.7wPt-635817.0wPt-4476 wPt-201319.0wPt-846319.3

w11747228.7

gwm34134.4

cfd6238.8BS0009805838.9wmc52241.0

barc32347.5

barc116151.9

gwm31455.5cfd12057.8BS0003727659.2wPt-626260.7cfd962.1wmc656 gwm383cdf965.0

BS0002191968.7gdm99a70.8BS0009849571.1

wPt-144076.2

wmc552 SNPPMOp1108885/3383.5gdm99c86.0BS0009748586.4

wPt-10668104.8

barc71116.5

wPt-9525139.5

3A 3B 3D

Sr27

Sr35

Sr51

Sr2

Sr12Sing

h et

al.,

P.C

Lott

a et

al.,

201

3

Yu e

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201

1Yu

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011

Rous

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201

4Si

ngh

et a

l., P.

C.Bh

avan

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l., 2

011

Bhav

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201

1Bh

avan

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011

Sing

h et

al.,

P.C.

wPt-9651 wPt-0433cfa2174

0.0

wmc4797.8gwm635a BS00022406BS00022082

10.6

wmc8311.7wPt-67217113.5wPt-8043 gwm33213.6wPt-237114.0barc100414.1barc7014.6barc2214.8wPt-920715.2gpw312715.4wPt-7448 wPt-163917.3wPt-666820.1SNPPMOp1069993/13 wPt-4748wPt742244

20.2

SNPPMOp410647/1621.8SyOpL119922.5wPt-6013 wPt-555823.3wPt-4553 wPt-096123.7BS0001098824.3BS0001387229.9wPt-525731.3barc15431.5barc12733.9wmc283.134.6wPt-9796 wPt-388335.7wPt-7186 BS0000255636.3wPt841839.0gwm6041.1BS0011111241.8wmc283_144.2wmc28344.4gwm47146.4wPt-74471549.8SNPPMOp2675910/36 cfa2028wPt-4515

52.4

SyOpL226253.9BS0002177163.1barc121_175.6BS00000847 barc12180.4wmc60781.4SyOpL232394.3BS0007817496.9SyOpL230098.6

7A

SyOpL896109.5cfa2019111.4wmc633112.4cfa2040_3112.6

SyOpL2086121.7cfa2040a wPt-8670123.4SyOpL1907124.1SyOpL1876126.5

BS00010809141.1

wPt-741686151.4

wPt-6495155.3BS00022560157.7

wPt-7763169.0BS00073988171.1

wPt-8846174.2

BS00011622178.6

BS00081108192.3

BS00023200203.3

wmc3230.0

barc27916.1

wPt-731829.6

gwm53740.0gwm573 wPt-7653gpw3188 gpw4444gpw7342 gwm46wPt-4863 wPt-0963wPt-4230 wmc335gwm297 barc289

53.0

barc276b56.6barc276c57.7wPt-7934 wPt-649860.5wPt-114966.5wPt-257267.7wPt-3873 wPt-637269.6wPt-2305 wPt031272.2barc101473.2barc27876.0wPt-3730 wPt-402577.7BS0001106979.2wPt-2994 wPt-227380.6BS0001050081.4BS0002210682.6wPt-735185.3BS0006573586.1wPt-182691.2wmc517 wPt-372392.0wPt-992593.9SNPPMM6777963/494.5gpw804098.7wPt-800799.3wPt-0600 SyOpL143wPt-3190101.5

7B

wPt-4258103.0SyOpL1352105.1BS00023023106.8SNPPMOp402574/42108.2wPt-5343 BS00010251SNPPMOp108828/50 SyOpL1351108.5

wPt-0194114.8BS00074359115.8wPt-2356119.0wPt-4300119.3BS00009464122.3BS00011065123.7

wPt-5462133.7gwm577136.0

gwm111141.2BS00010142153.3wPt-9488154.4

cfa2040 wPt-25650.0wPt-6652603.7gwm6358.3barc121_211.8gwm130 gwm437gpw519917.2

wPt-879821.1gpw114223.4barc128b25.9gwm47330.3SNPPMOp1043882/1631.5cfd21_132.9Sr5733.2w11733737.3BS0002246341.4wPt-1100 wPt-332845.1barc110445.2barc35246.8wPt-7171 SNPPMOp101458/70wPt-78947.4

csLV34 SNPPMOp1034549/1648.0cssfr5 wmc46349.3gwm42856.5cfd2157.3wPt-9822 wPt-2258SNPPMM62481791/38 SyOpL1368wPt-7763 wPt-731810wPt-7642

61.3

cfd6663.2gdm14565.0wmc15769.1BS00021987 BS00022511BS00022721 BS00022875BS00023045

69.2

BS00003945 BS00010071BS00012122 BS00021745BS00023184 BS00024032BS00024103

69.7

BS00009623 BS00011639BS00079734 wPt-205470.3

barc9772.0cfd4178.5barc121 wPt-1859gpw5192 wPt-671684.3

gpw7683103.1

wPt-3923 wPt-5674115.6wPt-664017116.5gwm635_1117.9gwm37120.3cfa2040b122.3

7D

Sr57

SrTA10171

Sr44

Sr25Sr43

Sr22

Yu e

t al.,

201

1

Sing

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al.,

P.C.

Yu e

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201

1

Bhav

ani e

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201

1

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sa e

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200

7

Sing

h et

al.,

P.C.

Sing

h et

al.,

P.C.

Sing

h et

al.,

P.C.

Hai

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201

2Si

ngh

et a

l., P.

C.

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200

7Cr

ossa

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007

Sing

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al.,

P.C.

Sing

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al.,

P.C.

Sing

h et

al.,

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201

1

Bhav

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201

1

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200

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Yu e

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201

1

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201

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201

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SrX

SrX

SrX

Ug99 Stem rust resistant gene linked to a marker.

Ug99 Stem rust resistant gene approximate location.

Non-Ug99 Stem rust resistant gene approximate location.

Toward a consensus map for Ug99 Sr Loci gwm156A gwm186cfd390.0

barc1411.3BS000221102.7BS000225003.3BS000112783.9wPt-10161 wmc713wPt-97484.4

BS000893576.9BS000111167.5BS000874538.7BS000511819.2BS000115379.7gwm293b10.3gpw213610.8cos5F11.6gwm63913.3wPt-356315.1gpw217216.0wPt-3334 wPt-190320.0wPt-523121.0wmc32727.9wmc52429.2SyOpL273133.8gwm29138.8gpw700739.4gwm304 wmc705wmc805 wPt-988740.4

SNPPMOp1978/35 PAPMOp10522140.5B1-5A SNPPMOp717499/31barc11741.3

PAPMOp57604141.9wPt-4131 BS00021708SNPPMOp314722/69 wPt-4249PAPMOp1059158 wPt-2768wPt-6048

42.0

SyOpL64342.3wPt-699044.0gmw30445.3gpw227350.4wPt-879455.0SNPPMOp1096021/1761.8gwm61762.3wmc41565.3BS0002941376.1

SNPPMOp115775/59110.3

wPt-5096 wmc727SyOpL778 wPt-733461126.6

wPt-5142144.9BS00082218145.4

BS00091080172.2

BS00010234196.5

5A

BS000092640.0

wPt-130219.8SyOpL283 SyOpL25020.5gwm234 BS00102828BS0001513622.5

wPt-840627.4wPt-387329.2wPt-114930.3wPt-534633.8wPt-158936.7SNPPMOp185131/4337.4BS0006261737.7wPt-258639.6BS0001891740.7SNPPMOp201244/17 wPt-4996barc442.9

SNPPMOp130896/1644.7gwm54445.5BS0010722248.4BS0003589954.2gwm21354.9gwm37157.5SNPPMOp445009/559.6barc7464.0BS0000387664.5wmc41568.5PAPMM610053770.7wPt-260772.8BS00091302 BS00011386wPt-125076.4

wPt-3457 SNPPMOp1472/5277.9wPt-935679.6BS0001095282.7SyOpL243784.2wPt-366185.4SNPPMOp332417/4490.6BS0000941495.1SNPPMOp468860/3395.3wPt-173396.4wmc289 SyOpL1005wPt-589696.5

wPt-601499.8wPt-3030103.1BS00000592104.2wPt-0750104.6gdm136b109.7barc275110.1gwm408111.7BS00022963112.3gwm604117.1SNPPMOp1640210/20 SyOpL1960SNPPMOp558187/29118.1

barc142118.5rPt-3114119.4wPt-9598 wPt-1482wPt-9103119.6

wPt-0935 wPt-9205wPt-8094120.0

wmc99124.0wmc235127.4barc232128.1barc266129.8gwm142135.5barc59138.5BS00003655140.6BS00021960144.3wPt-4418149.6BS00021709150.6wPt-0927153.6barc243155.6barc156158.7wPt-9815168.7gwm118174.4wPt-3922175.8wPt-9116 wPt-7665wPt-2373176.1

barc1120176.6wPt-0484177.4wPt-0837 wPt-9894wPt-4898 wPt-9027193.1

5B

wPt-5032 cfd180.0

Pina8.0Pinb9.4

wPt-622524.2

gwm358 BS0002210029.0BS00021901 gwm15929.6BS0004183830.2BS0002206733.6

gwm17456.2

gwm18260.2

SrHD85.3

gwm212106.5

BS00022228121.3

5D

Sr56

Sr53

Sr30

Bhav

ani e

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201

1

Bhav

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201

1

Lott

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201

3

Bhav

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201

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C.H

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012

Cros

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200

7

Kaur

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201

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gwm617_2 SNPPMOp1519638/550.0

wmc417_18.6

wmc57026.2

BS0001112545.0

PAPMM613738651.2wPt-1664 wPt-938251.9wPt-083254.4wPt-7475 wPt-137754.7BS0008363055.9wPt-66949860.4gwm334 BS00010933wPt-7623 wPt-6520

SNPPMOp10333/21wPt-4016

65.0

wPt-826678.2wPt-2573 wPt7127wPt-9690

86.0

gpw308787.7wPt-095988.7wPt-759989.1wPt-0357 wPt-8833wPt-3091

89.4

wmc807104.0

wPt-7373108.9

barc144112.9

BS00082104gwm570119.3

wPt-5601125.3

wPt-8662133.1wPt-5310134.8

BS00021965140.9

wmc417149.0

gwm617158.0gwm427162.8wPt-6696164.9BS00003339167.0wPt-4229 wPt-9474167.3wPt-1661 wPt-1642wPt-9976 wPt-4445

167.6gpw7433.2169.0wPt-7204 wPt-1695wPt-8124

169.4

wPt-6268173.8

wmc254211.0

wmc59219.0

wPt-2175 wPt-7745wPt-8721 barc1136wmc397 gpw10790.0wPt-3774 wPt-7662

1.0 BS00003785 BS00003792BS000097951.4gwm881.5wPt-3376 wPt-49301.9barc11592.2barc10932.5 gwm582.8 gpw5076 barc3544.2 wPt-95325.2 wPt-7777 wPt-35816.2 wPt-6994 wPt-4720wPt-4283 wPt-7150wPt-5234 wPt-2689wPt-0245 wPt-1852wPt-3304

6.6

wPt-72076.9 wPt-82397.0 wPt-49247.2 wPt-1922 wPt-3130wPt-9990 wPt-4386wPt-80157.3wPt-30608.9wPt-46489.2BS0001044311.1 gwm17413.4wPt-559629.9BS00010415 cfd13wPt-533332.3BS0000385233.0BS00022668 wPt-3733gwm518 barc1169wPt-2964 wPt-7636

44.5

BS0008696450.9barc14a52.8wPt-546153.2wPt-4164 wPt-9952wPt-611656.5wPt-3045 wPt-126457.2wPt-826857.5BS0001099357.7wPt-503758.0wmc15258.7BS0010903658.9BS0002318760.6wPt-602361.1

wPt-680264.4gwm200a65.0

wmc41778.6BS0002248079.5

wPt-5480101.9BS00011458BS00085589102.6wPt-0171106.8wPt-1541107.1BS00010618107.4wPt-9256110.1BS00023066110.4

BS00110651126.2wPt-732061128.1

BS00003785 BS00003792BS00009795 wPt-6994SNPPMM61159750/20 wPt-3774PAPMM6106536 wPt-7777wPt-7662 wPt-1922wPt-9532 wPt-4720wPt-4283 wPt-7207wPt-3130 wPt-1852wPt-9990 wPt-8239wPt-3304

0.0

SyOpL2811.0SyOpL22323.4SyOpL3624.6BS000104435.5SyOpL14577.3SyOpL1451 SyOpL191910.0wPt-3424 SNPPMOp1239709/6011.3SNPPMOp157697/3617.8wPt-217518.2SyOpL124718.5SyOpL1419.8wPt-559623.0BS00010415 cfd13SNPPMOp1227776/5026.6

wPt-533327.6BS0000385230.1

gwm518 SNPPMOp103652/2040.6SyOpL105141.2BS00022668 wPt-373341.5

BS0008696447.9wPt-546148.3SNPPMOp261873/8351.4wPt-503753.1BS0001099354.7BS0010903655.9BS0002318757.6wmc152 SNPPMOp3332865/80wmc417-2 SNPPMOp372691/2162.6wPt-680263.4SyOpL122573.4SyOpL74074.4SyOpL31375.0BS0002248076.5PAPMOp8618377.4wmc41777.6

BS00011458 BS0008558999.7wPt-5480100.9BS00010618104.5wPt-0171105.8wPt-1541106.1BS00023066107.4wPt-9256109.1

BS00110651123.2

wPt-732061127.1

6A 6B 6D

Sr26

Sr52

Sr13

Sr42 SrTA10187

Sr29

SrCad

Yu e

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201

1Lo

tta

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l., 2

013

Cros

sa e

t al.,

200

7

Sing

h et

al.,

P.C.

Yu e

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201

1

Bhav

ani e

t al.,

201

1Bh

avan

i et a

l., 2

011

Cros

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t al.,

200

7Yu

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l., 2

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Sing

h et

al.,

P.C.

Sing

h et

al.,

P.C.

Yu e

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201

1Yu

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Yu e

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201

1Yu

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l., 2

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Cros

sa e

t al.,

200

7Cr

ossa

et a

l., 2

007

Sr28

Sr13 Sr22

Sr40

Sr39

Sr33 Sr45

Sr35

Sr2

Sr21

Sr25

Sr26 Sr27

Sr29

Sr37

Sr42 Sr32*

Sr1RSAmigo

Sr50 Sr44

Sr43

Sr58

SrWeb Sr47

Sr51

Sr12

SrND643

Sr55

Sr56

Sr53

Sr52

Sr57 Sr46

SrTA1662

SrTA10187 SrTA10171

Sr31

Sr36

Sr32*

Sr32*

Sr30

SrCad

1A 2A 3A 4A 5A 6A 7A

1B 2B 3B 4B 5B 6B 7B

1D 2D 3D 4D 5D 6D 7D



Merged QTL

Merged QTL

Merged QTL

Merged QTL

Merged QTL

Merged QTL

Merged QTL

Sr30

Conclusions

Even with the growing number of characterized Sr genes - Eighteen possible Loci/chromosome locations to investigate - Possible new APR loci

Explained variance APR and genetic drag

-Evaluate the origin of the potential APR loci (i.e introgression/genetic drag) -Focusing the efforts on position with high variance (5B/6B)

Provides a consensus map for localization/fine mapping Sr genes - 1433 markers spread on the 21 chromosomes - Includes SSR but also GBS/DArT/SNP: New marker development

USDA Long-Xi Yu Matthew N. Rouse CIMMYT Sukhwinder Singh Ravi P. Singh Sridhar Bhavani Julio Huerta-Espino Cornell University Mark E. Sorrells

2014 bgri barbier presentation

Technology