2012-01-08 usama imtiaz 5312fg02-2 nanobiotechnology presentation

7/30/2019 2012-01-08 Usama Imtiaz 5312FG02-2 Nanobiotechnology Presentation

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TAS2012 Proceeding Presentation

Nanobiotechnology Fusion System2013-01-08

5312FG02-2 Usama ImtiazDepartment of Integrative Bioscience and Biomedical

EngineeringWB Group, Takanishi Laboratory

[email protected] 1


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Contents

1. MANIPULATING SINGLE PARTICLES USINGSTANDING SURFACE ACOUSTIC WAVES

2. 3D OMNIDIECTIONAL CONTOLLABLE ELASTIC IPMC

TWEEZER WITH SELF-SENSING AND ADJUSTABLECLAMPING FORCE ABILITIES FOR BIOMEDICAL

APPLICATIONS

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Paper Introduction

MANIPULATING SINGLE PARTICLES USING

STANDING SURFACE ACOUSTIC WAVES

Xiaoyun Ding, Sz-Chin Steven Lin, Sixing Li, Lin Wang, and

Tony Jun Huang

The Pennsylvania State University, USA

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Introduction

Manipulation of Micro/nano Objects e.g cells, bacteria, viruses, DNA etc.

Existing Manipulators and drawbacks:

Optical Tweezers High power laser, Complex, Expensive, Large

Magnetic Tweezers

Targets must be pre-labeled with Magnetic Material

Electrophoresis based Tweezers

Dependent on polarizability of particles,. Can adverselyaffect cell physiology

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Acoustic-based particle manipulation

Advantages: Non-invasive

Work for a wider range of microparticles regardlessof their optical/electrical/magnetic properties.

Significance of current work:

Focusing, Separation, Sorting and Patterning havebeen realized by acoutic methods before.

Authors have fabricated acoustic tweezers capableto precisely control single microparticles in two-dimensions

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Design Overview

A PDMS channel is bonded to a Lithium Niobatepeizoelectric substrate.

Two pairs of chirped Interdigital transducers

IDTs are also present on the substrate.

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Working

IDT pairs are independently fed RF signals togenerate surface acoustic waves (SAWs), whichinterfere to form standing waves.

Acoustic radiation force drives particles tonodes or anti-nodes depending on their elasticproperties, density, compressibility etc.

Position of nodes depend on the acousticwavelength xn=n/2

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Particle/cell manipulation in 2D

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Particle Manipulation

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Physiological Effect on Sample

HeLa cells were exposed to high power standing SAWfields for 6s, 1 min, 10 min.

The results indicate that there was no signifantphysiological damage induced to the cell

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Multiple particles

Simultaneouslylarge number ofparticles can bemanipulated.

Clusters of

particles can bearranged on asingle node ormultiple nodes.

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Conclusion

Small, Portable, inexpensive particle-manipulation system.

Can also be used for sorting (if combined with

flow-based microfluidics) Can dexterously manipulate single particles

Can handle multiple particles simultaneously,

grouped or individually. Have no adverse physiological effect on

samples

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Second Paper Introduction

3D OMNIDIECTIONAL CONTOLLABLE ELASTIC

IPMC TWEEZER WITH SELF-SENSING AND

ADJUSTABLE CLAMPING FORCE ABILITIES FORBIOMEDICAL APPLICATIONS

Guo-Hua Feng* and Jen-Wei Tsai

National Chung Cheng University, Chiayi, Taiwan

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Introduction

3D omnidirectionalaccessible IPMC elastictweezers

Can move multi-directionallyAdjustable clasping force

Self-sensing force by

feedback current

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Issues

Flexible Ionic polymer metal composites (IPMCs)have great potential as soft robotic actuators.

Conventional Preparation:

Ionic polymer formation

Compositing

Surface electroding

Usually prepared in large scale and simple shaped

IPMC Transducers: Planar IPMC structure withNafion sandwiched between plated metalelectrodes. (PolyTetraFluroEthylene and

Perfluorinated vinyl ether sulfonate)

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Fabrication

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Electrode plating

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Experimental setup

IPMC Tweezer can be inserted in the fixture Force balance

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Characterization Front-back movement

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Characterization Sideways

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3D IMPC tweezer capabilities

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Tweezer force and current relationship

Current flowing throughthe transducer can becalibrated to gauge thetweezers dynamic force.

Can determine iftouching/holding theobject and tosense/adjust clampingforce

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Conclusion

3D omnidirectional IPMC tweezer isdemonstrated

IPMC Transducer conmprises:

Motion element Clamping head

Can move multidirectionally

Can provide adjustable clasping force Has many applications

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2012-01-08 usama imtiaz 5312fg02-2 nanobiotechnology presentation

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