2 nat instt for geomics_adv biotech (nigab) pc-i page(22-29)

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    CO1TRNMENI OT PAKTSTANILANN1NC COMMTSSTON #ti,DEVDLOPMINI PROJECT

    NATIONAL INSTITATE FOR GENOMICS ANDAD'/ANCE D BIOTE CHNOLOGYNIGAB)

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    PC-1GOI'ERNMENI OF PA(ISTAIY

    PLANIYINC COMMISSION

    . NATIONAL INSTITUTE FOR GENOMTCS ANDDVANCED BIOTECHNOLOGY

    DEI'ELOPMEM PROJECI

    (NIGAB)

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    REI/ISED 2OOScornBelr ofp.ki.t tr

    Pc-lpt.mt4 Cohdt$ior(lrcductiotr S(or,

    l.N.bCOfTbFTOJCCi: NAIIONAI, INSTITDTE fOR GENOMICS .ANDADVANCED BIOTECENOLOCy (N'tcA!)Naroai Asndlhnal Reseeh cetrEe rN ARc L pek Road,

    t Nil"t:H:.'*^*.*.. t^.a.,r.od. Asricurtueand I nsa.L rsraniab.dlakish r',eiqJiwar R*NI CoDcit, Jd@bld

    Opcntiob rnd M.inreuBe : palisLn AgricuLnht ReeNh eoucil. tslMbadColcd.d Fedmr Milisr.rj MiDist y of tood, Aerj.ltnue !d rivesr@t, ht@brr,

    ') tt Oe pDj..r i! ,Dclud.d i, rD .dEa,. li". /.rh pt r. .pairy r..dlNAt) tlror ir.tuded h dr. & ftra pt d. n [,. qmon ft D.tusion rldtror utrtroQ propced ro be rrcodoodared

    The^projct viu b. nnied ttvolsh ?sDp for develoflns MrDnalgenohc .@ter ro qdrstmd the genone ofcop, nicrco* ra tooc mimas .iii) lfrh pmj.cr ir.preposd ro b. nn.(ed our orbrockrrovuotr ror. PDgr.,!, irdi(rt.:

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    om of rle tusr e.oMg alq, wbrch h6 a Dotentiar r. _._tuo aiia," br entui ra';!';; #]il;":;lff STH[I"":;H:ffi:':#::fl #ilf *tj1ilffi #:.:;l,i5:{imltrrns:i*::pl.'*',#dh#riT*""#$#\^HflTg,*H;ftT:J*l';: ffi"I "''*a'" ..'*^' i'r' ;.;'; t'";5. Prcjcl obj.ctives ard ft. rctltioNtip with Seclo.rt oblecF6:

    . Mgqdd rvalutid of smpidm or ieFrrant oops, micrcb* and foodQT! @pping h selected pldr ed food minals.::l*Tjil-,"| "1.d. rinrase rne senone nap. fo mpotut uaB &d@keF4rst d breedrne.

    I . Data ndoeeoent od devclopm4t of bio,infotualics tools.. Hlll@ Esole dvelopnent in plmb, eiaals ed mrcrobul snoniBlote.lDotoeyslducrs retftviding jmovatiE solutions io rh c@ial ewiomedal,agncuhft poblas.

    Dddopndl of gen-bded lhdpeltics.Identfi@rior ed cheacreriarion ofnicbbe dd fien xploiiation tlmuetr

    . IdeniificEtion of SNPS/DNA hurado.

    v&circs & diagostics for foodimportet genes oI dops, dimrs sdibr disas m&agnedl in ainals ed

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    $nLffi*:ilX":,Sf#,T;:H[rhar Fray ioloiar oie in gb\r,l._l:h* roa oci.,i'u*.-om( srdes,ss udersund re {a(tue dd M

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    DBcdprior ofrbe ptujed:al Crre bri.fbjrbrr. Fopord f&iltierdd t6ril.a1:onor th. prcjed;:.:T ;;: :1.i' ;.;,;[T,L*:tr;'"x#;ifl,ir:;? *:hl::.H,,** "D..krou l:

    jo(_ch,lltrCe" r r lI" cfln(J e iocRsed food ed hb"r pbducron, a ctemerlC-"tl'i*::{'.#;".-,%#".ffi Jffi :i".T",i:;*"tr.il^l::@dsru h m.erng *e SEwins needr ror tood, te.d rd a:oee or ind6Lldt E*fte,stui).ot eercn*. sl$ calkd g.nohics con:jys of mappurs. s.qu.ocrDg. ed:i':.ffi lfi.d:T',m"'i!&[il"":i:rHiil:;rft *iF"irui'ioDa eo Japtu. conon fUSA)i mda (JSAI ek . have benru*n;"v#, *#r#',,":' Iff I #J#;rrf l'#;:tnvlstlen6 D plar g.mmd @ d.velop $e ;o\.t hie\-rt oughpu goer.c maain!Hff#-efi:j tryfr".T*::" ;TT|.T,,:'tr ""y#trty"rffi#"11rtu6 $roxcn gmd r ngmeding dd motecL.d breeding sldregr$. Sidirdty, in caseor r@d ehdr senoahr. Lnlase eo \ohpsi,w tup! to, atri,,-,o* "p.ii*.1*.cen trEd. ryaldbte b pLbtic. r.'uousn inkhadoll co abomrotr _d itorr! Theeor ed oneohg (iwetopmd'of " bote senome seqre!.e oaps o I chick.ns. honeybe,@& od qiue sp*is is a maror sep foM&d S\p baed nap. now being ddet;pejmD $e crcrtu ad ca.te stuone squeb.ins prcj..6 ij Us 4 a d a-"ui.. # "immou" \atF n ?raluling Benetic oi,+uy ane* su*pribrt.q. gdorc pohu,llil,3iliffJ3;"lli haprds o' Qrr qd deveropmenr or o."n.rma mi.ar

    In $e ps fire yas. impoitu' concepb have emerged rrcm rquelcing pojecis:1'e#.::Tf;"T#1,' ff.?".,T,["?1.1.".x'ff *::'1. .p"i** "i,i,.*i.cRr.E!m. trd sne tu &*obr, ""+r*, c..i,Li"ii,?lipjl;H:.T:;FfiHTT Tbiid i'ed:.: Anort. inpotur lorce/ is rh" *" "' ,"I"p,*r. *q*".myi*. wrute a singte gtrome sequedce is 6ehrl. jb ulil.ddidomr Fqxerms batn. a..r,t,. c..o*",. c.#i*'ffi;:$H#:: :

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    It-l***#**nf*1'-*ill,,'*,s",-e*$ros:*,*lnm,**:"***.1;'n*,?.isf "Tffi #ff i:f,.{i,'#J$dfi crtiotr ot rh. pdiecrAgnculhR is tb. teadr0g eonomic se(tor oflakisBr dm.rn).uil.;l*Hr[t;i$""1iifi r].*fr j,ffi ili:"J"',T."'l:r;F;Hf;".'#x*;ii{l'*$dt'""Hfl"",H'""HIffi#rn";W.;ggt"ti,*;,q*;:,,fr ffi '#jf#ffi,r::ifu :N#*:+ftff $,q*ry1-r*g,5:"i:*ffifrt$i";i:nii+H'.-:'i:,:**'lri:ff ilf*,tll.*.,fr ,scr@ oed tor tle d*elorhtrrof sirrs @t*r dis"* resisla, ","p;-";i;;;;;ffi$#j;"T,il'.:?*:il"j:il*:ilff i:,':tT' ; ga;.n krn or e;,he.rlbnde 8@r ora.qu.nns hish Bb gqontr ,-r,T,'jli:T*::*1":lscrse aid hDtuM of 6er) gene ,h tu orgr sm rtared rosgncrihn{:Cjffi $jg6;,j"'iti#h*1}i{Jgffi*,Ha[x:d]]rnnn*:Jrr-Hiili*-:lifl:*n.;x:m;^n;

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    sEucnml gmrics-snrdi4 of d sr.udue dd orgeiaton of gdones; 2) n&cilionatstuds{hlds tbal Etare 8d.h" ,hc-" -" oed." lo pt4r MclloD al theelJule,ktel: ad Jt appt,.d ge1om.(s apoticanon ot $e sqomj! inbmLioD tudxnoqledae for de\elopDlr o, ijlprcved bi;als & pttuls dd 0reu produs s R NItre detelopmeot olrew dags Md d6gnos( techniqcs.Stncturrl gcroEtcs rBqrch jvotve! .trr dd,ng dre !tuture ed oryItlufion ol8trom.s R.fdh in rhjs ft..o!td j,cjDd. Lb. D.od-knrqpEsed squ@. rae. (EsD no; ;##;;,,f;,1ji,'ffi x*xxfffdjlgtructi_o!of ph*_icl naps (cloned ed ord.Ed secnqits of th. g;. n;;;PndE y.s,.p would bf ! de(lop B.n?(ic ha+s;or dra,o! crop ptuB rD pallshm,mngah,-i- r.. dd olhse.onomicjlt) hpobr species. MeJ orde motecurfj).1i1..1,11:h l:+"t*. bioti, sdabioLic {h"es esihirein mon pru6 Ar^ff &"-. Tfl : :i";ft:: jH[ ;: TLi:[ H i#;tr;Jk]f ?.?T:l:I g*:", ,n ercqh dd deterormol o.*.- -jy,,, or,r.,_uo -a;iilfi iiil il.;il"ff .:: ffi ffi ;T,trr [H:, Ti]ti,:ltii;TL1il"urrxab@ at intamdjm bon boej*l nodels r ,ppried pl&. .^p.*r"it p^g*Batd on$e prvioG knoqtedge bd ou findings, Ewro, ne d,selje Fs,e(_m{i sM" "*"*.. "*" "*: :";'ff"} ffiJ.:^flTSilfrtrx':;Ti;:ffi.":"f1':;Tjrd*t.l;;-*i il: ^.. ;''h* e.;;' ;

    Gmeric cbalmn4Litr-dd Lhe e(,enr of eseric d^ftn) ee uJ_.-._: ll:a,1lT .' .4..rT ed suqnaoiirry ol oop md rrvdoci pDducr@iaboDtones in rhe wortd chaerena gemp.Lm 6ins motadetu\s DNA n&(ea cq dhungui:h semlt6h e6siotu: urefoE, cuttrt6 ddhyDaii.r b. id.,L4trd slt.Ip.@lplerbFedcrnste atso. r ru.qLen*so, de DNArrcblej usually ind.(aks po$ibte oe" gene. in Ge collecr,on U4sificadotr ott::j*1.:1.1:" -,,r D\a Fskm c-ene,arry aeres wirh ,h,r of no. or;ci;::1T1.-1'Tll* TlF,: *y!rcvid. srea,d rsoruuob qrh *" .,.i1" i",i rr,"ndtJ. Benetic .tircsiry winin ea *ong1,llu,* o,::l\:"p! ''a5' T. "'y,"q *ins conrodonar DNA Mknc lucb N RAfD, ssR &l..ilif l n"tt-"*1,.^ ,.*n 6ins hish thrcushpur ,ys.m ". ..q,.";.s 6;;".n{! lerp b de\elopr b,e gs,tc jidrse ru'J. jsoj,rne OlLs d gpies-Mrh 4ooeMisdisjcuhtodiscorerli.|- $e@n\;roL g"""irol.o..yr,"l

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    ffi F;'g*'iffii$#*kfi-il't.t:"i-l+r'+*."ooo-"uy ua u,cogi"arry i.r;;# ;"T;l;: "ff l{H;ff :: ::ffi fi n.li*'ssrid"m.,:"tr $#i**i,.9ffi ggll[,. ru ij#r{i, jT+:lF:!Ii ilT:l"-.fi :r;T$:f f":lr il"Hilrtrt, :i;lff "i"tH,,[?,3.,.'ji,11ft ;1i,,1il,*1,:: gil;".*,;#;'il;r ;il',T*',:#'i"T ;'" -".Lil;'*',"T:il"",H.,y., *e hu.,ion o, d,a@,m*-.d$i-:f rTJiilis"i;;#"-;"TT:1i"ffi :il""T,il:rTrtre;$ffigffiffii:dtr$.]t,trffiffil'fii?.:i:-i[]#.ffi fi iil*fu ,xfi#*"{:r##:ffi :#{"ti.F:!Fil6:i.r*il #.x;;r;"*i: ;rr, i"rir*. *;e pFrdr sludy. a 6r.rmnal g.Mk plat m wid bffi !}-li51iifll-r,lkltfiffi h-",hry.Fj*ffirt#"":'ff"ffi l#*ril!Hft"#j$-' r'.* rsr, ssi. *:ris,*::""1 mti l"*ir;:": ffi ;:;i'fl fl:JhTr,i l."ii"I :il%::

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    Fo^tiftulor djffe@uauq. fl._c.,t inrs&tiorrj J) senoac{0vrcmeDr irbEc,,onff ifti:#ffi *l'ff ;i;:jr:Tt"-"riil"tiii*$.i;i,-:#ff*ffiffi$'{$ j*i{Liil*gffi $"T:'3","*"Tr.ff ;$*:,T*;The pos-equncing em q Dove Epjdb &om crudetv;r*'.n*h,:.**lrrff ff*lJ";l$lli:ilr?."xtT"#,r;m"'nni *i,l*5i#*+,*,* [1'Si#6Tr-o.. s"qm*s io, ugn.,,rr,r +.:;; "iir ;,6riJ,rflLrry;fi#i,i,;,f, :lffijf.ffi iils;;.#tl"*"rr*::u;,,{",u:** n*itr,}il6:Hr#';trr.*iix:fliil,HH;"x##i:[f::x,i+ltrl*,#:rtfi*{ffi :fl,##'fi#ft Iffi [*htretuh genome daE \.tlorkre sLh tbese ed orher enil& etrorr, witi e;;*:?111ffi*":rs,rfi #:i#,{l}"****rffi 'ffi xin*'"-;kn:t'il,-*',.rt'r:ff*trf:fi'"{,ltuide.rjl biolosicat prokcs.s ir pturs. ncrobes ed eira.., ".i;JJ;;;;ifi;;Gtre

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    Fj,,,:il.,,i1"t_T1,fx,!ff ,ii,T;;":::H#:.ff r'*,#.;tH:uo os.ae Es'!b!c. udf dific,al) creaed poDds eDv,tumorAT:K:'xffilffi *ii:i:?i*',J";',T""htri -e bdeEtud,he sh.n*6sh_dd po*y, dd J'm@o",;r., ",,;; ;ii;ff1"?ffi"y# :'J,iHl:;fi:"ffi.j;"'j,#:k1"l;;,1_:;:ff;.*:]i" *, p.'a. u d*.r t;,inrrcvrm*' oreind &d a;a-,*. p,.d,;;;).;;;##trH,frTtr t;:ffifiA,;:trd"T*Tff::,1 l"5t1".,lll" r;,,r,r .il",;r"e".- -iPBOJECT OUTPUTS:Expectd ouFuts/ouiones sd benelirs ofrhe proposed pojecr kdd be:a) A funcrional gnomica iciitute Mllstu{,16 rc$dins Dolcute evalMron

    esdl m *tung !p ofworkine elurE enessed inor mJor c.ops tud dr'mt spares for in!rcvingt) Di*6e csi@r snd sN$ rspoNle eener q be identified tud Eanstom.d uingcr G.netic drs.r) !resfl in rhe tcjl tood mro' bedj srcn 6 bunajo dd r hjct.nwill be.elucidiRd wlnlh wi be fo oqed by idenliFar.on otgftes affe{ring ritlprcduaion ed ihoseinvotred in dree Esis;ced)Goetically nodified plals rsist nr to sress (drcusht, satl , reDp.erc.) will be oadee) A vdie,t, ofsen.,bded .tisesc diEsnosrics wil be mde availabt. for miEal healthil"#tr1Tf"""t r:g::1

    y nodned vacci.es wr beooe svairabre againsi bird0 Sme new molecdes wil b*one available lo sery s gene based 6eE!.utics forgl A dab-hN reesdnB gene leq@ces ot someud m'crcbes wjllbe made atailabte Io, pubtrc use idportant gn s Eon llmls, mirElthrcugn bioinfotutiG sys&ns.

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    LEA]IONITORABLE TARGETSUANTIFIAB NITORABMol6ul& .valudiion dd QTL4d,5is of 3lr.$ rcspootvegene in scl.otd mim.ls,

    Qudifiqtion oi em.lic dive6ity dd QrI-sid.dincrdon ror bioiic (dk6., in*ct dc)md abiotic (droughr, 3au, bmpeEturc) *.s.sin mjor crops. In 6. ordone$icd.d eiml.(isu]dine tiv.sbqk, goulty {d tuh), di*aesp.cific fr,J*.6 6 w.n goqh .nhdcingmal*6 rill be idhti6.! for qploidio't

    Identifidionchmr.ddion oi b.8.n.dgendfNwmdtionsEnifiose in simals, plonissNPs ed rh.ir asocidion w1& di!.acrcsinr&dsrpribiliry .nd drug efiic&y iiminals will b. inv.srisacd Ii c6. ofricro&s, g.n. nurdiod in arid influ.nzvns, FMD virus vill b. srudi.d in li.ldi3old!r, ror dryelopine new vec'nesgrcvnvFoduotion, dis@c loloMoqiBctoedire, drcuht, slr, will be idediliedand firn *pdsion qill b. [email protected]*elopme.r of frolecul& PCq RT.PCR, and NA{eqEnc. b6.ddi.sn6ds will be develop.d teiitur 4 *lci.danimd dl$d$ sd would t. mdc avail.blcfor pubLic of pivlb iBriruiional usage.

    DNA tinselpriniin! ofhdirional .nd inpov.dcuhivd or dificEnt $danim.h (crnk, Poulq, nsh.r.) dd.rs l*kd, rc, dc)DNA pan$n aad Pdfilcs oi vmou. copctrhiv@ rid mimal bEds *ould be availablcfor identifdion ind uiiliarion in bcedids

    Developm.nt of tmS.nisplmb (*heat n&, .rc, fordisw. isocr. drousht rdc.ndically modified plats or givei c@ps

    D.velopoeli ol DNA b6.dv&cin6 sgaiGt :elecEd d;ilca y modincd as"i*( dr 161 2 dajorfdon dniml dis!ffi (FMDV, Av iiflu.M)will b. made availa6G ior Public or priwl.O4oia $niid,woi6hoptconfrtrceylEinLn lb traiiing wo.lshopv seminad confeEd.e

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    7. Crpit t co.t esliDrtq. ldtcar. drte of Brinrtior ot proj.ct coc( :toth M@h, 2OO7C.pitl Cod ofPbjecr:Pbjed Lmal Co$: 482 70t Ojj jon Rs)0 000

    (Pleas. @ hbto I otr rhe ndr p!se)

    ;"ffi:'"":*::H:T"ff"fi ,ilJ,Tii.:fiil iilil;1lii.rq hrke, rnv,ln thrs prolecl @y @oponMrs ee invotved which ddatto,.ro"ur.,a.,r..,. *rtur. *. ;; ;;i; ;;;i ;#":"f;i,,#:fl:i; H:lill"#fl:tlf,T "#Il..i:iln pa],i-kn m _1, ; nnpon;. 'l. -. r*ii.aiffi t"'0" "*,* or Pnvric.t lcri'iris by h,iD codpo!.rrs $ per

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    " Fo he purchose otcommercio kijs to corry olt DNA puoricotion, seque.cing,:l:rlis,11d exp$tof ociiviries. rhis oso ifcrldes pu.chose or orher iobreogenho.d chemicokwhich dre noiprodlced LocorLi $ *. *ry ";p. ;.b) Asency (uit sise cost): N.a.

    Comuic,uon Chdc$udiioes. osce $r'!6n;;36;-i

    33 t28

    orher soices (Dpr. Audi ehjlsdorrg chae*( sem inrG;;!:G;Ghot;

    )29.570 26.A42Otrce fquipDenl & Cohpul;

    l;ilJotu nsb bui rd,E;rd;R; ho&442.70) l00.om

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    231.733Hitus oi kcnniG !i;E proc@;i;i equiPmenls, vehicl6 as4eyrs oi l]leMol.culr ch@G;;i;i of *iea &d rie cing la?D atrd SSRo*aop.mr .. -p.r*u ro q I L nappE *r I be i^ik[;.

    C@rinued DNA-b6ed chi@tenar\o! of qo! plols(Dhar, ice), eddd micmbes (Avie Intu@ viro andU.I"";:t'T#Rm;ffi :"'"=-*.-'"*"'ulaton developneni for OTL oi.til:'j:;,';1.1ffiTm:, _",T",t "-rft d-*=il l;dsequocrng ra acveropitni6Tlili@iiG" ro-m;-dlfi @*io"ees rrainine oisadrisGld

    conshcB for fie ielded eeret rotded bydevelopd by i@nbiDmt DNA

    esblishnenr of BioinJomh6 ati.civilworl w tconti.le.

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    lbair3 lrCrptkt cNt b. $ork.d our or rh. b,.h orc,ch i..n orsork ! rE..drDovrtrd prcvid. irtoroadon r! p.r to o,ira.ple6e see Aeqe I & rl

    8, A4u.l opentirg rrd brinr.drn.. c@i aftr coDpl.hon orrbeprejcr.

    9. DeErrd .rd luppty ndysi!Acn ul:T F ire mror.dic?rre fero, ro rhe aonomy of par.sb. popLra-.on so*dt.h P!tustan h ulsd e., + b ( h n..ds add. oo"t jo;o I esorks we de slplosed bmajor !rcps dd' e I'veskpr*'Ldim'|o

    Iroqron or rlle moriid DNA ma*eB for rh;;;GD molado ltdi & mtnalse,qumcae ortheE?iiEreiiriili;nriiacobMued dekropre;i;i;;R\ ;'fud.rtt*;ffi;iE G c1hDara tualysi! denved 6;E[d; srucual ma ftucronat ge;;;l;;,t'rc!8h bio-ilfomatis cn.

    aevetopmor oi pnyroffiiE- for eiml path;i;El!:l:l*::" T9 c\dmklarro;;a,asekd e.nes , r,c!L sr!o$ae, etc) aid inen erpesion mlvsis (Conrnue ID.u"rop..n, or DNA b;Ai;;;Effi--iti,vtE "^dTiRntinuesESTs. of .rc. e,y fttry f-.i*M;"c rha *"1conrinuea ae"eropmeni-iiiffi G ror the 6ota'ed c.;sElG;;a by

    s2,068Milio! Nil 52.068Millio!oluuftuer cosr = r).z85Miuioiopdtional Cost = 36.783Miuion]:d =52.068 Milliot

    EsrabiishnE .ost= l5-t3friiti;;Crpqalional Cosl = 36.783Miition&tl = 52.068 Mittio.

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    OFFICE EAUIPfiENTsrt I -E;l-ld;+ffi#;ffiffiPicePerunii2 0075a 0050I 0 0202 0.124 a 248l2 0 500 0 5000 025a 0 0050 s00 0 5002.01A

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    LIST OF VEHICIES

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    IIIIII Cost per Square Fset Rs 1500.57 per Sq.FrIItITI

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    IIIIIIIIIIIII

    LIST OF EQUIPMENT

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    Pafttd Asn.ulNnl Reseadh Courcrl,sedor G.5. htdrabBd

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    a ir) B'eak'up or Rs:t&!-sjll:-on ol Annuat Recudin8 cosr aner donptetion of 5yds oi E poje.t is given below. Il is dlicipated rbal the saldies, .on ofconsumbles, utilit'cs ed other expenses will be inoeaed sienificlnlly.

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