REV PORT PNEUMOL IV (4): 435-437

19° Congresso Annal da Sociedade Europeia de Micobacteriologia (ESM-98)

Lisboa, Julho 22/25, 1998

19th Annual Congress of the European Society of Mycobacteriology (ESM-98)

Lisbon, July 22/25, 1998

Resumos/ Abstracts

(Continua~ao)

Follow-Up of SMEAR Positive Pulmonary Tuberculosis (TB) patients during treatment: can Cobas amplicor PCR be used in a Semi-quantitative manner to acess the efficacy of treatment?

V. 0. THOMSEN1, A. KOK-JENSEN2• S. PHILIPPI-SCHULZ3,

H. J. BURKARDT3

1 Dept of Mycobacteriology Statens Serum lnstitut, Dept. of Pulmonary Medicine

3 Rigshospitalet, both Copenhagen, Denmark, and Roche Diagnostics (3) Kaiseraugst, Schwitzerland

t•U•U .,.. • .._..... , -..-r, wln.n ... tea n.lu • n.,bat..-y ..,.c:L.-.. .._..._.. • • ~,. ... , • ..-·u., , .... ~ .c ll ft ,..ct...u....,.. .......,...... tea.. .. ,.. .. rt ......

_.t .. C!loiM.- ,..uow ' "' ... -.uuca.u• ot Cia teo- " · t.._C'C\I.lod a (MTII _. • latarul lMi~lU.• -uol CIC.'C'I . Att•~ ~l'l41Ntto. to p~a .,..clUe fN Mft ... MCit ra.,.c;tlwtv. t M opdcel ._.ley (OD) o r ...ch or t M hY't-l'i4iu.tt.oa p~u w• ... a-wnd.. 11M nuo Mt-... the opUca l dilaaltloa (Nft/MO:I wu catc:uht.cl .

Julho/ Agosto 1998

...__lu otK-.. ... A4't"Ll let. un t.O "*r Ut:lt Uti -..r. iecl'"'-4 • SuU.ucal ....t,.,. f nak con-et.u .. eo.ULeu:ac b,- apu_,...t ~ t.hat ..,../ICC ntio corn .. laud. a t.,.a.Uuady becur wltlil _.t &ad wlt .. wlt\ln c•.n.le.a t.Ma tbe KTW 00 va l\le &looe. W'hea t:orr.tatlft9 c.o .... _,. 9-l' ... lftl l h . l•. 1•. •• .._,ati.,...L •u tn'S/ ICC l'atioe ..,.'* 1 . 1. 1 I. l . S. 1 . 1. ...,.. o. s. ,...,.c.dY'ely. ro.r cvltu.n n•. 1•. • . t~ef.atl.,.t . M&a XR/lCC ratio• V~tr. 1 .2. l . O. o . t . aftd O. l . 1"'1--epect.Lvely • ._.. COifiP4-*"'-' ....tv••• at dtftete.a.t t.'--t ~ t.f'N.c-aat • ._. tOWid. t.'- rollowL.ot n-.lu,,

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Oaaclv.tton. • 1'tla "leulu ahow that. .a t ... u,.a po• u•ve dtutnt a loew .... , .. •h..u ltutladon of u•.au•u.c aM Lftdlc.u• ttu.t. Cobaa ,_,hcor Pea ,l'fln M u.a.-4 l• a ,.,__, . qu.&lltha.tl.ve ...,_..c to -Lto:r t b. elf tea~ ot tnat .. ~l

Evaluation ofMmb Redox Medium for Growth detection of Mycobacterium

E. CAMBUA. C. WICHLACZ, C. TRUFFOT-PERNOT, V. JARLIER

National Reference Centre for Surveillance of Mycobacterial Diseases and Drug Resistance. Laboratoire Central de Bacteriologie-Hygiene, Groupe Hospitalier Pitie-Salpetriere, Paris, France

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435

19Q CONGRESSO ANUAL DA SOClEDADE EUROPElA DE MICO.BACTER10LOGJA (ESM-98) RESU MOS/ ABSTRACTS

The Influence of environmental Mycobacteria in the cell mediated immunity against tuberculosis

F. L. L. CARDOSO. A. MacFARLANE, H. M. DOCKRELL

London School of Hygiene & Tropical Medicine, University of London. UK

EnYiroatMtltll m~~ on wSciety Nw!b!Md ill NNrrC. l'bcR IS • trrpxc:s~s lbat OICX II)'Pk:ll mft'Ol*tm• cart c:oacn.Ootc 10 tiMWttl_,. api1u1 ll.lbcn'ulotis Md Mpdwd)' lnnUM« ~tit PfOW'C'Ih·c dfrct ot BCG utclnc The mycobacwna IP«ia. ltiC dose of the l•'lltum 1M ttle r~M~k ofapotu~ (Wialas.oa. tlll.t$o,.ln'- .a;"' ~) can be dinnc a'l4•c usoaa~td o;o•lVllhc: ditn:.au an.d \he IIMSi,idtal life ayk. lolafaoo-aamma (lr'N·&) IS • ervc:MI C)tokinc for ~c xth•ioo ....Sis wotl.ai.Cd ...,fill prot«boff t,p.lM IUibero.dosis. O'Pf' I Unmwx tnpC~ruoe. IL--10 1-' • r)10tltlc with a poitAt Wub'IOI)' ctrca on M~a., lhc Ni• ctrcetor ttlb Oft dw IQ)'cobiCICril uuu.,.. Tbc: IUlhots tNdKd UK «II I'IICdtaud imm-.mc fc:$pCIQM (CMH in vitro to 1 CIIVilrOIItMAW tn}cobKtcria. •

Objcat\ U.: l..()uerminc: \he lympbop"olifcnrvc fts;pcmot 10 PfO from 1 "'""'~ M}'cobldcria. l•MA~~nclw.produc:tjon ofiFN-t and IL·IO

M~ Ptriphual blood IMIIOIIIKklt cdh (PBMC) •m: isolKt:d (totr~lhc blood of 10 btal~ do~MJr~, Cellt v.m l~ for l day$ ..nt. PPO of M.l'iin MJwtcdhdatc, M1iCrOil.ll&cutn.. M fortUU\A M ~ M.,.KCX, M~ and M.eubcm.ltMU. L)'lflpftoproliCcnrioCI "'._, ~ by JH 1.hyrn.icllt~t lt~Corpotat.lon. lfN"' and 11.-10 w~ tkter:m:iDed by WSA .ift lhtPB.MCwpa'lloiUtlt

Jlctuk;: M )CIO{\IliCMI\. ~t.e'ritdll. lftd M.~Uu:Juc- pn hlabc:,. tymp,optOflftta~tOa and tF'N·s produe1ion. bul kM 4dfcrcnca bci"C'Cfl tht spcc~n wac 1101 JUiiMICilly )lanifK-MI

DnciiWIO!l M ~r~>ffoll.ccWil. M &ViUift .wid M lnuxciJ~~oiJtC- alk1 Lno.,n u ,\t;\IS ffWP. ••~c4 \ftc h1~ rc:.pon.tc 11t1 d!d t l'lld)

Alt*V! dlcrc W» no 1>tatistital sl.a:ruf!C'&n«. ibcK rcsulu UA be c:\P~ b) lhc bltb uou rtatlhl1) of antiJCM bcot'lletft tbcK l JP«In.. llld b)' tlw ptotr..bk pWomiaull CftYitot~mml&l OIIOSIJfC 10 them 'The '"'" indloiC1icM or IFN·a pnM:I\ICtion by thac: my(b~ wortiq IJ • rw\lftJ Yxtiac:, eM tn&$ lhc CMI to 1)1)( 1 mpon~t, lhal tJ I»QCCI&ed 'A'It)l proc«tioft ap.iMl tubcra.!.Mis..

Differential Cytokine and Proliferative responses to challenge with Live and Killed Mycobacterium bovis BCG

E. VALADAS2, F. ANTUNES2, FREITAS ECOSTA2, T. SERRA3,

J. POCAS4, F. VENTURA~, H. M. DOCKRELL1

London School of Hygiene & Tropical Medicine. London, UK Hospital Santa Maria, Lisbon, Portugal · C. D. Pneumol6gico, Venda Nova, Lisbon, Portugal Hospital S. Bernardo. Serubal, Portugal Hospital Egas Moniz, Lisbon, Portugal

Sp.-citlc:: ruin&n« to Mycob•cu_rt._ t-ul:lcrculoeh 1o.t.ct1011 h ooly dUchr:n.ly gene.nted with live, but not with ktlled t..eteri• . Tbie •un••u thet the antige n• neeenuy t o ge.n•r•t• c••ht • .ac. •r• el~u-le by liv• an4 -t.&boltcally AC'Uw baC:t•rl• , but net by kU hd org-anb•• ·

Obj.C:t1YUI To .. t • blhh vtln~r u~ and k llhd .. . bovi• aco indue• different ptolihr.a tlvc a nd qotokine rupor~au .

Kater'iala 4 KetbocS.s hdph• r • l blood .onCJn-.Jclur c:• ll• f.,_ '111\tna t • d tuberculo.et.s p.atiant• • itb puliiOMry and u:tnpul~~GM.ry cUauaa, a.n4 t roa be.4Llc.hy ac:o vacdn•t•d controh •n• e ti-..laud dtbu vtt.b Uve or Jtillad U rradiated) M. bovta aoo. Th}"Udtn. lncor:pon.don and cytoktlla produc:tloa ttnr-g~ TWP'-a .. and IL-10) wn: ••••~d .

Reaultaa 0\U' a t.tildy da.onatr:ate• • 19Q-i Ucant dUfar.a~• U t.be Ahlllt;y of llv. or kil led M. bcwU ICO pnpuationa to • licit T•cell p r:olUandoa aAd crt-olllDe p rod\lcd.on . Liv• bacu~:l• were 110ra efUclent t.o i~ctSon of' proUt• .r:adoa. t:nr-t. 'DfF·•· aftd tL-10 than the equivalent kill•d preparAtion. tbh dUtertDC:• ••• obfia.l'Yed in both. tuberwloal• .,_thnta and httallh.y controh .

Conclu.ton• ; ThU• relulta a-uggut t.hal llYii M. boY-.la 1m aac:tete• a.ntigena vblch induca T c•Jl prolihnti on and cyt~ine production by both 1' calla &ft4 eonocyt .. .

Julho/ Agosto 1998

Mycobacteriosis in Hospital de S. Joao (HSJ): Patients, strains and susceptibilities

M. RJBEJRO. D. PINHEIRO, J. H. CORREIA DA FONSECA, F. RODRIGUES, E. MARTINS

Lab. Microbiologia, HSJ. I.N.S. Rjcardo Jorge. Porto. Portugal

~ON Tbt ra&ptdiCIC 01 6i:iCiiiWOiiS m ibC 1a11 diDc:adc IDd u.e i:Dc::n:ate o1 ~ bobca: atbc:r tbatt M.vcoboettritl-'lf tllb~rr:vltUis ~~ boea tiap:.DU_y OlD.QICICSed with HJV illrccUoa. Ia this rcpon. wt review M>"CCiboclcN lsola1o !Jam our bbonool)' ....s lhdt ~for-,_. MATEIUAI. AND ME'TllOOS: Sampla fn>m - biolosical pndM:tl -. p..- llld -....s iJI MiddlcbiUII: 71113 or Middl- 7Hll brotb. or Ia .............__ (lJ) aad..,. .......,.,.t -tly for a iOial of

six 10 dglu "oW. Tile~ DNAprat>ca Ol die daloit ~-- ia U...,. UO<dl'or die iclcauficadoo oldie ..,.;,. 1o die I'O'ilh~ C>ll .... (l>olii!Yt - for me - of add-fall bocilli), To acc:o1o .l l>v:obocttnvm tvkt<Vlosis c.,.pln 'II<<) """''''>'bililies SIRE~~ JWampida. Elhambucol) io ll><:l<c 400 TB S),..,.. ,. ... usecl; tbc or11tr SU>ins...,.. ......,...S wido IDdidoul-- for SIRE, c,doscrloc. ri!:ll>utio. ~amioooalicylic ac;d. <tlliooamiGt. bDaooydo. --~~~~~~ ~ RESUl.lS: A lllUl of J59 samples from 162 poll<~>t.s """' pooii:M, or ...._, 71 (43.1 %) bdorl& 10 1a!o::1ioois o. ...... llq>outm<ot. ploXou ...... """ IIIOaly HlV I'O'i!M. Til< ............. pmc.u bad - admitud 10 die Pllc:wnololl)' (SS, l~ 0%), lmcmal Melioaoe !25, IS.~%) lllld orlltr ..,.._(II, 6.1%). Mr< - iloLMal Ia 94.~% of tbc po<icJti, "llile Myc-•., .,.;.., '"""f'fa (.W«) - - ;. die .......... '-6%. SIISC>qltibilil)' l<:llio& pafomcd Ia 100 tll1lim of All< mal doc followUoa ... "'"- iooai.ajd aad strqllom)-cio. 4,(1%: lsorliazid ooly, 1.0%: ~ ooly, 1.0%: .., ioooiod<l. n&oopcm aad lllqliOIII)'Cio UW. lbc olher ....U.. (91.0%) did""'- clnl& rca-, N 10 .... u,.- lhe -'bili!Yialioc­P<f(omcd Ia 6 ....U..: I l<'eolcd _..wed resi5uoce 10 all clnlp a.d s ...,. ,..;dYe., cyeloo<rinc Ollly CO:<CUJSIO:<S. 'l'h< I<SU!u cmpl®><tlh< IUaJ><r pm~I<DO< ot.ll« ....U.. Ia polieaaa "hom fn:quonO~ IDV potUI\'e lllq· ~bo $how t.h.ll fi~ line dtu& rcsisunce ts prac:nt Ua a% I.Dd ODiy a .,, multidru& resiJI.anc:lt •• obs<rvod lo ~· •ilb pmious rqx>IU~fac" lhc """""-rn..-.lsol.ll&.

Vol. IV NQ4 437