18 wahyuningsih, r. 2011. tempe, superfood yang...

18

Wahyuningsih, R. 2011. Tempe, Superfood yang hebat. http://www.detikfood.com/read/2011/07/17/123106/1682904/900/tempe-superfood-yang-hebat?9922022/. [14 November 2011]

Winarno, F.G. 1992. Kimia Pangan dan Gizi. Jakarta : PT. Gramedia Pustaka Utama Zainal. 2005. Kajian Pemanfaatan Air Kelapa Menjadi Minuman Ringan Beraroma

Nenas. Jurnal Sains & Teknologi, April 2005, Vol. 5 No.1: 37-49 ISSN 1411-4674.

LAMPIRAN

Lampiran 1. Skema Kerja Penelitian

Belut

Tepung Belut (Purwanto, 2012)

Tempe diberi penambahan tepung belut dengan konsentrasi 0%, 0,75%,

1,5%, 2,25%, dan 3%

Diuji kadar air (AOAC, 1970)

Diuji kadar protein total

metoda Kjeldahl

Diuji kadar lemak

(Sudarmadji, dkk,, 1997)

Diuji angka ketidakjenuhan

metode Hanus (Sudarmadji,

dkk,, 1997)

Uji organoleptik tempe

meliputi tekstur, aroma,

rasa, dan warna (Soekarto,

1985)

Lampiran 2. Analisa Sidik Ragam Kadar Protein Tempe dengan Penambahan Tepung

Belut

Ulangan Konsentrasi

Tu X u du Wu kontrol 0,75% 1,5% 2,25% 3%

I 18,18 19,86 20,15 20,4 20,58 99,17 19,8340 -0,0580 3,7360

II 18,2 19,9 20,2 20,47 20,61 99,38 19,8760 -0,0160 3,7824

III 18,26 19,94 20,25 20,48 20,67 99,6 19,9200 0,0280 3,7498

IV 18,28 19,97 20,2 20,54 20,69 99,68 19,9360 0,0440 3,7511

V 18,22 19,91 20,24 20,47 20,63 99,47 19,8940 0,0020 3,7769

Tp 91,14 99,58 101,04 102,36 103,18 497,30 Too

X p 18,2280 19,9160 20,2080 20,4720 20,636 19,892 Xoo

dp -1,6640 0,0240 0,3160 0,5800 0,7440

SD 0,04147 0,04159 0,03962 0,04970 0,0445

SE 0,05149 0,05164 0,04919 0,06170 0,0552

Keterangan: Tu = Total Ulangan; Tp = Total Perlakuan; T,, = Total Umum; X u = Rataan Ulangan; X p = Rataan

Perlakuan; du = X u - X oo ; dp = X p - X oo ; SD = Simpangan Baku; SE = Kesalahan Baku taksiran,

W(1) = (x0%)(dp0%) + (x0,75%)(dp0,75%) + …(x3%)(dp3%)

= (18,18)(-1,664) +(19,86)(0,024) + …(20,58)(0,744) = 3,736

W(2) = (x0%)(dp0%) + (x0,75%)(dp0,75%) + …(x3%)(dp3%)

= (18,2)(-1,664) +(19,9)(0,024) + …(20,61)(0,744) = 3,7824

W(3) = (x0%)(dp0%) + (x0,75%)(dp0,75%) + …(x3%)(dp3%)

= (18,26)(-1,664) +(19,94)(0,024) + …(20,67)(0,744) = 3,7498

W(4) =(x0%)(dp0%) + (x0,75%)(dp0,75%) + …,,(x3%)(dp3%)

= (18,28)(-1,664) +(19,97)(0,024) + …(20,69)(0,744) = 3,7511

W(5) = (x0%)(dp0%) + (x0,75%)(dp0,75%) + …(x3%)(dp3%)

= (18,22)(-1,664) +(19,91)(0,024) + …(20,63)(0,744) = 3,7769

N = (W1)(du1) + (W2)(du2) + (W3)(du3) + (W4)(du4)+(W5)(du5)

= (3,736)( -0,0580) + (3,7824)(-0,016) + (3,7498)( 0,0280)+ (3,7511)( 0,0440) + (3,7769)(0,0020)

= 0,00038816

D = (Σdu2)( Σdp

2) = 0,0063 x 3,7593= 0,0238

JK Non Additivitas = = = 6,3176, 10-6

FK = observasi

2..)(=

25

)30,497( 2

= 9892,2916

JK Total = (18,18)2 + (19,86)

2 + … + (20,63)

2 – FK = 18,8342

JK Ulangan =

perlakuan

Tu 2)( - FK

= 5

49461,6166- 9892,2916 = 0,03172

JK Perlakuan = ulangan

p

2)(- FK

= 5

49555,4396- 9892,2916 = 18,79632

JK Galad Acak = JK Total – JK Ulangan – JK Perlakuan

= 18,8342 – 0,03172 – 18,79632 = 0,00616

DAFTAR SIDIK RAGAM UJI ADDITIVITAS

Sumber Ragam Db JK KT F Hitung F Tabel

5% 1%

Ulangan 4 0,03172

Perlakuan 4 18,79632

Galad Acak 16 0,00616

Non Additivitas 1 6,3176, 10-6

6,3176, 10-6

0,0154 4,54 8,68

Sisa 15 0,0061537 0,00041025

Kesimpulan : Data Additif (F Hitung < F Tabel)

DAFTAR SIDIK RAGAM


5% 1%

Ulangan 4 0,03172 0,00793 20,5974 3,01 4,77

Perlakuan 4 18,79632 4,69908 12205,4 3,01 4,77

Galad Acak 16 0,00616 0,000385

Total 24 18,8342 4,707395

F Hitung > F Tabel

Kesimpulan : Antar ulangan dan perlakuan berbeda nyata

KV = ..

.

AcakGx 100 %

= x 100 % = 0,09863974%

Uji Beda Nyata Jujur (BNJ) 5%

BNJ 5 % : W = q α (p, n2) Sx

Sx = 5

000385,0 = 0,00877496

W = 0,00877496 X 4,33 = 0,038

Protein

0% 0,75% 1,5% 2,25% 3%

X ± SE 18,23±0,051 19,92±0,052 20,21±0,049 20,47±0,062 20,64±0,055

W=0,038 (a) (b) (c) (c) (d)

Keterangan : *Huruf yang sama menandakan tidak ada perbedaan yang nyata

** Huruf yang berbeda menandakan ada perbedaan yang nyata

Lampiran 3. Analisa Sidik Ragam Kadar Lemak Tempe dengan Penambahan Tepung

Belut

Ulangan Konsentrasi


I 9,4 10,5 11,15 13,52 14,62 59,19 11,8380 0,3048 18,5605

II 9,3 10,39 11,05 13,38 14,5 58,62 11,7240 0,1908 18,4530

III 8,8 9,05 10,45 12,67 13,69 54,66 10,9320 -0,6012 18,5179

IV 9,5 10,61 11,27 13,62 14,78 59,78 11,9560 0,4228 18,6987

V 8,9 9,94 10,58 12,81 13,85 56,08 11,2160 -0,3172 17,5971

Tp 45,90 50,49 54,50 66,00 71,44 288,33 Too

X p 9,1800 10,0980 10,9000 13,2000 14,288 11,533 Xoo

dp -2,3532 -1,4352 -0,6332 1,6668 2,7548

SD 0,31145 0,6388 0,36291 0,43134 0,4865

SE 0,38665 0,79305 0,45053 0,53549 0,604



W(1) = (x0%)(dp0%) + (x0,75%)(dp0,75%) + … (x3%)(dp3%)

= (9,4)(-2,3532) +(10,5)(-1,4352) + …(14,62)(2,7548) = 18,5605

W(2) = (x0%)(dp0%) + (x0,75%)(dp0,75%) + …(x3%)(dp3%)

= (9,3)(-2,3532) +(10,39)(-1,4352) + …(14,5)(2,7548) = 18,453

W(3) = (x0%)(dp0%) + (x0,75%)(dp0,75%) + …(x3%)(dp3%)

= (8,8)(-2,3532) +(9,05)(-1,4352) + …(13,69)(2,7548) = 18,5179

W(4) =(x0%)(dp0%) + (x0,75%)(dp0,75%) + …(x3%)(dp3%)

= (9,5)(-2,3532) +(10,61)(-1,4352) + …(14,78)(2,7548) = 18,6987

W(5) = (x0%)(dp0%) + (x0,75%)(dp0,75%) + …(x3%)(dp3%)

= (8,9)(-2,3532) +(9,94)(-1,4352) + …(13,85)(2,7548) = 17,5971


= (18,5605)( 0,0348) + (18,453)(0,1908) + (18,5179)(-0,6012)+ (18,6987)( 0,4228) + (17,5971)(-0,3172)

= 0,369130555

D = (Σdu2)( Σdp

2) = 0,7701x 18,3654 = 14,1437

JK Non Additivitas = = = 0,0096338

FK = observasi

2..)(=

25

)288,33( 2

= 3325,367556

JK Total = (9,4)2 + (10,5)

2 + … + (13,85)

2 – FK

= 96,065144

JK Ulangan =

perlakuan

Tu 2)( - FK

= 5

16646,0909- 3325,367556 = 3,850624


p

2)(- FK

= 5

17085,9737- 3325,367556 = 91,827184


= 96,065144 – 3,850624 – 91,827184

= 0,387336



5% 1%

Ulangan 4 3,850624



Non Additivitas 1 0,0096338 0,0096338 0,382595 4,54 8,68

Sisa 15 0,3777022 0,025180147


DAFTAR SIDIK RAGAM


5% 1%

Ulangan 4 3,850624 0,962656 39,76521 3,01 4,77

Perlakuan 4 91,827184 22,956796 948,2949 3,01 4,77

Galad Acak 16 0,387336 0,0242085

Total 24 96,065144 23,9436605

F Hitung > F Tabel


KV = ..

.

AcakGx 100 %

= x 100 % = 1,349068858%


BNJ 5 % : W = q α (p, n2) Sx

Sx = 5

0,0242085 = 0,069582325

W = 0,069582325X 4,33 = 0,301291469

Lemak

0% 0,75% 1,5% 2,25% 3%

X ± SE 9,18±0,39 10,10±0,79 10,9±0,45 13,2±0,54 14,29±0,6

W=0,301 (a) (b) (c) (d) (e)



Lampiran 4. Analisa Sidik Ragam Angka Iodium Tempe dengan Penambahan Tepung

Belut

Ulangan Konsentrasi


I 62,04 64,01 65,49 67,46 70,91 329,91 65,9820 -0,0212 41,3521

II 62,54 64,51 65,98 66,97 70,91 330,91 66,1820 0,1788 37,8516

III 62,54 63,52 65,49 67,46 69,43 328,44 65,6880 -0,3152 34,0123

IV 62,04 64,51 65,98 67,46 69,43 329,42 65,8840 -0,1192 33,9632

V 62,54 64,51 65,98 67,46 70,91 331,4 66,2800 0,2768 38,5175

Tp 311,70 321,06 328,92 336,81 351,59 1650,1 Too

X p 62,3400 64,2120 65,7840 67,3620 70,318 66,003 Xoo

dp -3,6632 -1,7912 -0,2192 1,3588 4,3148

SD 0,27386 0,44331 0,26838 0,21914 0,8106

SE 0,33999 0,55035 0,33319 0,27205 1,0064



W(1) = (x0%)(dp0%) + (x0,75%)(dp0,75%) + … (x3%)(dp3%)

= (62,04)( -3,6632) +(64,01)( -1,7912) + …(70,91)( 4,3148) = 41,3521

W(2) = (x0%)(dp0%) + (x0,75%)(dp0,75%) + …(x3%)(dp3%)

= (62,54)( -3,6632) +(64,51)( -1,7912) + …(70,91)( 4,3148) = 37,8516

W(3) = (x0%)(dp0%) + (x0,75%)(dp0,75%) + …(x3%)(dp3%)

= (62,54)( -3,6632) +(63,52)( -1,7912) + …(69,43)( 4,3148) = 34,0123

W(4) =(x0%)(dp0%) + (x0,75%)(dp0,75%) + …(x3%)(dp3%)

= (62,04)( -3,6632) +(64,51)( -1,7912) + …(69,43)( 4,3148) = 33,9632

W(5) = (x0%)(dp0%) + (x0,75%)(dp0,75%) + …(x3%)(dp3%)

= (62,54)( -3,6632) +(64,51)( -1,7912) + …(70,91)( 4,3148) = 38,5175


= (41,3521)( -0,0212) + (37,8516)( 0,1788) + (34,0123)( -0,3152)+ (33,9632)( -0,1192) + (38,5175)( 0,2768)

= 1,783773723

D = (Σdu2)( Σdp

2) = 0,2226 x 37,1393 = 8,2671


FK = observasi

2..)(=

25

)1650,1( 2

= 108910,5603

JK Total = (62,04)2 + (64,01)

2 + … + (70,91)

2 – FK

= 189,891344

JK Ulangan =

perlakuan

Tu 2)( - FK

= 5

2544558,366- 108910,5603 = 1,112984


p

2)(- FK

= 5

2545481,284- 108910,5603 = 185,696584


= 189,891344– 1,112984– 185,696584

= 3,081776



5% 1%

Ulangan 4 1,112984



Non Additivitas 1 0,384881199 0,384881199 2,140691 4,54 8,68

Sisa 15 2,696894801 0,179792987


DAFTAR SIDIK RAGAM


5% 1%

Ulangan 4 1,112984 0,278246 1,444601 3,01 4,77

Perlakuan 4 185,696584 46,424146 241,0254 3,01 4,77

Galad Acak 16 3,081776 0,192611

Total 24 189,891344 46,895003

F Hitung > F Tabel


KV = ..

.

AcakGx 100 %

= x 100 % = 0,664929424%


BNJ 5 % : W = q α (p, n2) Sx

Sx = 5

0,192611 = 0,196270731

W = 0,196270731 X 4,33 = 0,849852267

Angka Iod

0% 0,75% 1,5% 2,25% 3%

X ± SE 62,34±0,34 64,21±0,55 65,78±0,33 67,36±0,27 70,32±1,006

W=0,850 (a) (b) (c) (d) (e)



Lampiran 5. Analisa Sidik Ragam Kadar Protein Tempe dengan Penambahan Tepung

Belut

Ulangan Konsentrasi


I 61,05 61,14 61,2 61,33 61,35 306,07 61,2140 -0,1104 0,1475

II 60,91 61,02 61,21 61,45 61,8 306,39 61,2780 -0,0464 0,4305

III 61,03 61,12 61,39 61,57 61,89 307,00 61,4000 0,0756 0,4210

IV 61,12 61,22 61,45 61,67 61,85 307,31 61,4620 0,1376 0,3663

V 60,88 60,95 61,25 61,42 61,84 306,34 61,2680 -0,0564 0,4676

Tp 304,99 305,45 306,50 307,44 308,73 1533,1 Too

X p 60,9980 61,0900 61,3000 61,4880 61,746 61,324 Xoo

dp -0,3264 -0,2344 -0,0244 0,1636 0,4216

SD 0,10035 0,10583 0,11314 0,13312 0,2237

SE 0,12458 0,13138 0,14046 0,16526 0,2777

Keterangan: Tu = Total Ulangan; Tp = Total Perlakuan; Too = Total Umum; X u = Rataan Ulangan; X p = Rataan


W(1) = (x0%)(dp0%) + (x0,75%)(dp0,75%) + … (x3%)(dp3%)

= (61,05)( -0,3264) +(61,14)( -0,2344) + …(61,35)( 0,4216) = 0,1475

W(2) = (x0%)(dp0%) + (x0,75%)(dp0,75%) + …(x3%)(dp3%)

= (60,91)( -0,3264) +(61,02)( -0,2344) + …(61,8)( 0,4216) = 0,4305

W(3) = (x0%)(dp0%) + (x0,75%)(dp0,75%) + …(x3%)(dp3%)

= (61,03)( -0,3264) +(61,12)( -0,2344) + …(61,89)( 0,4216) = 0,4210

W(4) =(x0%)(dp0%) + (x0,75%)(dp0,75%) + …(x3%)(dp3%)

= (61,12)( -0,3264) +(61,22)( -0,2344) + …(61,85)( 0,4216) = 0,3663

W(5) = (x0%)(dp0%) + (x0,75%)(dp0,75%) + …(x3%)(dp3%)

= (60,88)( -0,3264) +(60,95)( -0,2344) + …(61,84)( 0,4216) = 0,4676


= (0,1475)( -0,1104) + (0,4305)(- 0,0464) + (0,4210)( 0,0756)+ (0,3663)( 0,1376) + (0,4676)( -0,0564)

= 0,019591266

D = (Σdu2)( Σdp

2) = 0,0422 x 0,3666 = 0,0155


FK = observasi

2..)(=

25

)1533,1( 2

= 94017,05088

JK Total = (61,05)2 + (61,14)

2 + … + (61,84)

2 – FK

= 2,240216

JK Ulangan =

perlakuan

Tu 2)( - FK

= 5

7470086,308- 94017,05088 = 0,210856


p

2)(- FK

= 5

1470094,419- 94017,05088 = 1,832936


= 2,240216 – 0,210856– 1,832936

= 0,196424



5% 1%

Ulangan 4 0,210856



Non Additivitas 1 0,024827428 0,024827428 2,170273 4,54 8,68

Sisa 15 0,171596572 0,011439771


DAFTAR SIDIK RAGAM


5% 1%

Ulangan 4 0,210856 0,052714 4,293895 3,01 4,77

Perlakuan 4 1,832936 0,458234 37,32611 3,01 4,77

Galad Acak 16 0,196424 0,0122765

Total 24 2,240216 0,5232245

F Hitung > F Tabel


KV = ..

.

AcakGx 100 %

= x 100 % = 0,18067746%


BNJ 5 % : W = q α (p, n2) Sx

Sx = 5

0,0122765 = 0,049550984

W = 0,049550984 X 4,33 = 0,21455576

Kadar Air

0% 0,75% 1,5% 2,25% 3%

X ± SE 61±0,12 61,09±0,13 61,30±0,14 61,49±0,17 61,75±0,28

W=0,215 (a) (ab) (bc) (c) (d)



Lampiran 6. Uji Organoleptik Tekstur Tempe Dengan Penambahan Tepung Belut

Tekstur Tu Xu dU W

Panelis A B C D E

Keterangan : Tp = Total Perlakuan; Tu = Total Ulangan; Xp = Purata Perlakuan; Xu = Purata Ulangan; SD =

Simpangan Baku; SE = Standar Error; (X,,) = Purata seluruh data panelis

1 3 2 2 3 3 13 2,6 -0,624 0,088

2 3 4 3 4 4 18 3,6 0,376 0,208

3 4 4 4 4 4 20 4 0,776 -3,553E-15

4 2 3 2 4 3 14 2,8 -0,424 0,184

5 2 3 3 2 3 13 2,6 -0,624 0,008

6 2 2 2 3 2 11 2,2 -1,024 -0,024

7 4 3 3 2 5 17 3,4 0,176 0,232

8 4 4 3 3 3 17 3,4 0,176 0,152

9 4 4 4 4 4 20 4 0,776 -3,553E-15

10 4 4 3 3 3 17 3,4 0,176 0,152

11 4 3 4 4 4 19 3,8 0,576 -0,136

12 4 4 4 4 4 20 4 0,776 -3,553E-15

13 4 4 4 4 4 20 4 0,776 -3,553E-15

14 4 4 3 3 3 17 3,4 0,176 0,152

15 2 3 3 3 4 15 3 -0,224 0,08

16 3 3 3 4 3 16 3,2 -0,024 -0,024

17 3 4 4 5 5 21 4,2 0,976 0,056

18 3 3 3 4 4 17 3,4 0,176 0,072

19 2 3 2 1 2 10 2 -1,224 0,16

20 3 4 2 2 2 13 2,6 -0,624 0,288

21 3 4 2 3 4 16 3,2 -0,024 0,456

22 4 4 3 3 2 16 3,2 -0,024 0,056

23 3 2 3 3 3 14 2,8 -0,424 -0,136

24 3 2 3 3 3 14 2,8 -0,424 -0,136

25 4 4 3 2 2 15 3 -0,224 0,08

Tp 81 84 75 80 83 403 T00 Xp 3,24 3,36 3 3,2 3,32 3,224 X00 dP 0,016 0,136 -0,224 -0,024 0,096

SD 0,7789 0,7572 0,7071 0,9129 0,9

SE 0,32 0,31 0,29 0,38 0,37

N = (W1)(du1) + (W2)(du2) + …+ (W25)(du25)

= (0,088)(-0,624) + (0,208)(0,376) + … + (0,08)(-0,224) = -0,2328

Σdu2 = (-0,624)

2 + (0,376)

2 + … + (-0,224)

2 = 8,3456

Σdp2 = (0,016)

2 + (0,136)

2 + …+ (0,096)

2 = 0,0787

D = (Σdu2)( Σdp

2) = 8,3456 x 0,0787 = 0,6570


FK = observasi

2..)(=

125

)403( 2

= 1299,272

JK Total = (2)2 + (3)

2 + … + (4)

2 – FK

= 81,728

JK Ulangan =

perlakuan

Tu 2)( - FK

= 5

6705- 1299,272= 41,728


p

2)(- FK

= 25

32531- 1299,272 = 1,968


= 81,728 – 41,728 – 1,968 = 38,032



5% 1%

Ulangan 24 41,7280 Perlakuan 4 1,9680

Galad Acak 96 38,0320 Non Additivitas 1 0,0825 0,0825 0,21* 3,9533 6,9458

Sisa 95 37,9495 0,3995

Kesimpulan : *Data Additif (F Hitung < F Tabel)

DAFTAR SIDIK RAGAM


5% 1%

Ulangan 24 41,7280 1,7387 4,39** 1,634 2,026 Perlakuan 4 1,9680 0,4920 1,24* 2,482 3,548

Galad Acak 96 38,0320 0,3962 Total 124 81,7280 2,6268

Kesimpulan : ** Antar ulangan berbeda nyata, * Antar perlakuan tidak berbeda nyata

KV = ..

.

AcakGx 100 %

= x 100 % = 19,52%


BNJ 5 % : W = q α (p, n2) Sx

Sx = 25

0,3962 = 0,13

BNJ 5 % = 0,13 x 3,94 = 0,496

Tekstur

0% 0,75% 1,5% 2,25% 3%

Purata ± SE 3 ± 0,29 3,2 ± 0,38 3,24 ± 0,32 3,77 ± 0,36 3,87±0,39

W = 0,496 (a) (a) (a) (a) (a)


Lampiran 7. Uji Organoleptik Rasa Tempe Dengan Penambahan Tepung Belut

RASA Tu Xu dU W

Panelis A B C D E

1 2 2 3 3 3 13 2,6 -0,568 0,136

2 4 4 4 3 3 18 3,6 0,432 -0,104

3 4 3 3 3 3 16 3,2 0,032 -0,088

4 3 2 3 2 3 13 2,6 -0,568 -0,024

5 2 3 4 3 3 15 3 -0,168 0,12

6 2 2 3 4 3 14 2,8 -0,368 0,208

7 3 4 3 2 3 15 3 -0,168 -0,12

8 3 3 3 4 4 17 3,4 0,232 0,104

9 3 4 4 4 5 20 4 0,832 0,12

10 4 3 3 3 2 15 3 -0,168 -0,12

11 4 3 4 3 4 18 3,6 0,432 -0,024

12 3 3 4 5 4 19 3,8 0,632 0,208

13 4 4 5 4 4 21 4,2 1,032 0,032

14 4 4 3 3 3 17 3,4 0,232 -0,136

15 2 2 2 4 3 13 2,6 -0,568 0,176

16 4 4 3 3 3 17 3,4 0,232 -0,136

17 4 4 4 4 5 21 4,2 1,032 0,032

18 1 1 1 5 4 12 2,4 -0,768 0,384

19 3 4 1 1 2 11 2,2 -0,968 -0,288

20 2 3 2 3 3 13 2,6 -0,568 0,056

21 2 3 2 3 3 13 2,6 -0,568 0,056

22 4 4 5 3 4 20 4 0,832 -0,04

23 4 2 4 2 2 14 2,8 -0,368 -0,112

24 3 3 3 4 2 15 3 -0,168 0,04

25 3 4 4 3 2 16 3,2 0,032 -0,048

Tp 77 78 80 81 80 396 T00 Xp 3,08 3,12 3,2 3,24 3,2 3,168 X00 dP -0,088 -0,048 0,032 0,072 0,032

SD 0,9092 0,8813 1,0408 0,9256 0,866

SE 0,38 0,36 0,43 0,38 0,36



N = (W1)(du1) + (W2)(du2) + …,,+ (W25)(du25)

= (0,136)(-0,568) + (-0,104)(0,432) + …, + (-0,048)(0,032) = --0,0998

Σdu2 = (-0,568)

2 + (0,432)

2 + …, + (0,032)

2 = 7,9744

Σdp2 = (-0,088)

2 + (-0,0,48)

2 + ...+ (0,032)

2 = 0,0173

D = (Σdu2)( Σdp

2) = 7,9744 x 0,0173 = 0,1378


FK = observasi

2..)(=

125

)396( 2

= 1254,528

JK Total = (2)2 + (2)

2 + … + (2)

2 – FK

= 103,472

JK Ulangan =

perlakuan

Tu 2)( - FK

= 5

6472- 1254,528 = 39,872


p

2)(- FK

= 25

31374- 1254,528 = 0,432


= 103,472– 39,872– 0,432 = 63,168



5% 1%



Sisa 95 63,0958 0,6642


DAFTAR SIDIK RAGAM


5% 1%

Ulangan 24 39,8720 1,6613 2,52** 1,634 2,026

Perlakuan 4 0,4320 0,1080 0,16* 2,482 3,548

Galad Acak 96 63,1680 0,6580

Total 124 103,4720 2,4273


KV = ..

.

AcakGx 100 %

= x 100 % = 25,61%


BNJ 5 % : W = q α (p, n2) Sx

Sx = 25

0,6580 = 0,16

BNJ 5 % = 0,16 x 3,94 = 0,64

Rasa

0% 0,75% 1,5% 2,25% 3%

Purata ± SE 3,08 ± 0,38 3,12 ± 0,36 3,22 ± 0,43 3,24 ± 0,38 3,2±0,36

W = 0,64 (a) (a) (a) (a) (a)


Lampiran 8. Uji Organoleptik Aroma Tempe Dengan Penambahan Tepung Belut

AROMA Tu Xu dU W

Panelis A B C D E

1 4 3 3 4 4 18 3,6 0,184 -0,088

2 4 4 3 4 4 19 3,8 0,384 0,016

3 4 4 3 3 3 17 3,4 -0,016 -0,032

4 3 3 3 3 2 14 2,8 -0,616 0,016

5 3 3 3 3 3 15 3 -0,416 0

6 3 3 4 4 4 18 3,6 0,184 0,032

7 3 4 5 5 2 19 3,8 0,384 0,216

8 4 3 3 3 3 16 3,2 -0,216 -0,136

9 4 4 4 4 5 21 4,2 0,784 -0,016

10 4 3 3 4 3 17 3,4 -0,016 -0,072

11 3 4 3 3 3 16 3,2 -0,216 0,104

12 4 3 4 4 4 19 3,8 0,384 -0,104

13 4 4 4 4 3 19 3,8 0,384 0,016

14 4 4 3 3 2 16 3,2 -0,216 -0,016

15 2 3 3 3 3 14 2,8 -0,616 0,136

16 2 3 4 4 3 16 3,2 -0,216 0,184

17 4 4 4 4 5 21 4,2 0,784 -0,016

18 3 3 3 4 4 17 3,4 -0,016 0,048

19 3 4 2 2 4 15 3 -0,416 0,04

20 2 4 2 2 4 14 2,8 -0,616 0,176

21 3 4 4 3 3 17 3,4 -0,016 0,088

22 4 4 4 5 4 21 4,2 0,784 0,064

23 4 4 4 4 4 20 4 0,584 0

24 2 3 3 3 3 14 2,8 -0,616 0,136

25 2 3 4 2 3 14 2,8 -0,616 0,056

Tp 82 88 85 87 85 427 T00 Xp 3,28 3,52 3,4 3,48 3,4 3,416 X00 dP -0,136 0,104 -0,016 0,064 -0,016

SD 0,7916 0,5099 0,7071 0,8226 0,8165

SE 0,33 0,21 0,29 0,34 0,34



N = (W1)(du1) + (W2)(du2) + …+ (W25)(du25)

= (-0,088)(0,184) + (0,016)(0,384) + … + (0,056)(-0,616) = -0.2968

Σdu2 = (0,184)

2 + (0,384)

2 + … + (-0,616)

2 = 5,2736

Σdp2 = (-0,136)

2 + (0,104)

2 + …+ (-0,016)

2 = 0,0339

D = (Σdu2)( Σdp

2) = 5,2736 x 0,0339 = 0,1789


FK = observasi

2..)(=

125

)427( 2

= 1458,632

JK Total = (4)2 + (3)

2 + … + (3)

2 – FK

= 66,368

JK Ulangan =

perlakuan

Tu 2)( - FK

= 5

7425- 1458,632 = 26,368


p

2)(- FK

= 25

36487- 1458,632 = 0,848


= 66,368 – 26,368 – 0,848 = 39,152



5% 1%



Sisa 95 38,6597 0,4069


DAFTAR SIDIK RAGAM


5% 1%

Ulangan 24 26,3680 1,0987 2,69** 1,634 2,026

Perlakuan 4 0,8480 0,2120 0,52* 2,482 3,548

Galad Acak 96 39,1520 0,4078

Total 124 66,3680 1,7185


KV = ..

.

AcakGx 100 %

= x 100 % = 18,69%


BNJ 5 % : W = q α (p, n2) Sx

Sx = 25

0.4078 = 0,13

BNJ 5 % = 0,13 x 3,94 = 0,503

Aroma

0% 0,75% 1,5% 2,25% 3%

Purata ± SE 3,28 ± 0,33

3,52 ± 0,21 3,4 ± 0,29 3,48 ± 0,34 3,4±0,34

W = 0,503 (a) (a) (a) (a) (a)



Lampiran 9. Uji Organoleptik Warna Tempe Dengan Penambahan Tepung Belut

Warna Tu Xu dU W

Panelis A B C D E

1 3 2 3 3 2 13 2,6 -0,76 0,12

2 3 3 3 4 3 16 3,2 -0,16 1,332E-15

3 4 4 4 4 4 20 4 0,64 1,776E-15

4 3 3 3 3 2 14 2,8 -0,56 0,12

5 3 3 4 3 3 16 3,2 -0,16 0,04

6 3 3 3 3 3 15 3 -0,36 1,332E-15

7 3 3 4 3 4 17 3,4 0,04 -0,08

8 4 4 3 3 3 17 3,4 0,04 0,08

9 4 4 4 4 4 20 4 0,64 1,776E-15

10 4 4 4 3 3 18 3,6 0,24 0,12

11 3 4 4 4 4 19 3,8 0,44 -0,08

12 4 4 4 5 4 21 4,2 0,84 1,776E-15

13 5 4 4 4 4 21 4,2 0,84 0,08

14 4 4 4 4 3 19 3,8 0,44 0,12

15 2 3 3 3 4 15 3 -0,36 -0,2

16 4 4 4 4 4 20 4 0,64 1,776E-15

17 4 4 4 4 5 21 4,2 0,84 -0,12

18 1 1 1 2 4 9 1,8 -1,56 -0,36

19 4 3 4 2 1 14 2,8 -0,56 0,36

20 3 2 4 2 2 13 2,6 -0,76 0,16

21 4 4 2 4 3 17 3,4 0,04 0,04

22 3 3 3 3 3 15 3 -0,36 1,332E-15

23 4 4 4 4 4 20 4 0,64 1,776E-15

24 3 3 2 3 3 14 2,8 -0,56 -0,04

25 4 4 3 3 2 16 3,2 -0,16 0,2

Tp 86 84 85 84 81 420 T00 Xp 3,44 3,36 3,4 3,36 3,24 3,36 X00 dP 0,08 0 0,04 0 -0,12

SD 0,8206 0,8103 0,8165 0,7572 0,9256

SE 0,34 0,33 0,34 0,31 0,38


Simpangan Baku; SE = Standar Error; (Xoo) = Purata seluruh data panelis

N = (W1)(du1) + (W2)(du2) + …+ (W25)(du25)

= (0,12)(-0,76) + (1,332 x 10-15

) + … + (0,2)(-0,16) = 0,1504

Σdu2 = (-0,76)

2 + (-0,16)

2 + …, + (-0,16)

2 = 9,2

Σdp2 = (0,08)

2 + (0)

2 + … + (-0,12)

2 = 0,0224

D = (Σdu2)( Σdp

2) = 9,2 x 0,0224 = 0,2061


FK = observasi

2..)(=

125

)420( 2

= 1411,2

JK Total = (3)2 + (2)

2 + … + (2)

2 – FK

= 82,8

JK Ulangan =

perlakuan

Tu 2)( - FK

= 5

7286- 1411,2 = 46


p

2)(- FK

= 25

35294- 1411,2 = 0,56


= 82,8– 46 – 0,56 = 36,24



5% 1%



Sisa 95 36,1302 0,3803


DAFTAR SIDIK RAGAM


5% 1%

Ulangan 24 46,0000 1,9167 5,08** 1,634 2,026

Perlakuan 4 0,5600 0,1400 0,37* 2,482 3,548

Galad Acak 96 36,2400 0,3775

Total 124 82,8000 2,4342


KV = ..

.

AcakGx 100 %

= x 100 % = 18,29%


BNJ 5 % : W = q α (p, n2) Sx

Sx = 25

0.3775 = 0,12

BNJ 5 % = 0,12 x 3,94 = 0,48

Warna

0% 0,75% 1,5% 2,25% 3%

Purata ± SE 3,44 ± 0,34 3,36 ± 0,33 3,4 ± 0,34 3,36 ± 0,31 3,24±0,38

W = 0,48 (a) (a) (a) (a) (a)



Lampiran 10. Kadar Lemak Tempe Dengan Penambahan Berbagai Konsentrasi Tepung

Belut

Ulangan Pengukuran Konsentrasi

0% 0,75% 1,5% 2,25% 3%

I

m. sampel 5 5 5 5 5,01

kolf awal 48,22 165,77 106,68 165,79 106,70

kolf akhir 48,69 166,30 107,24 166,47 107,43

% lemak 9,4 10,5 11,15 13,52 14,62

II

m. sampel 5 5,01 5 5 5,01

kolf awal 48,23 165,79 106,68 165,80 106,72

kolf akhir 48,70 166,31 107,23 166,47 107,45

% lemak 9,3 10,39 11,05 13,38 14,5

III

m. sampel 5 5 5 5 5

kolf awal 48,23 165,81 106,69 165,81 106,72

kolf akhir 48,67 166,26 107,21 166,44 107,40

% lemak 8,8 9,05 10,45 12,67 13,69

IV

m. sampel 5 5 5 5 5

kolf awal 106,69 165,81 48,24 106,70 165,81

kolf akhir 107,17 166,34 48,80 107,38 166,55

% lemak 9,5 10,61 11,27 13,62 14,78

V

m. sampel 5,01 5,02 5 5 5

kolf awal 48,24 165,81 106,69 165,82 106,72

kolf akhir 48,69 166,31 107,22 166,46 107,41

% lemak 8,9 9,94 10,58 12,81 13,85

Contoh perhitungan :

% lemak =

Contoh pada ulangan I konsentrasi 0%:

% lemak =

Lampiran 11. Angka Iodium Tempe Dengan Penambahan Berbagai Konsentrasi Tepung

Belut

Ulangan Pengukuran Konsentrasi Vol.

titrasi

blanko 0% 0,75% 1,5% 2,25% 3%

I

m. lemak 0,25 0,25 0,25 0,25 0,25

20 vol. titrasi 7,4 7 6,7 6,3 5,6

angka iod 62,04 64,01 65,49 67,46 70,91

II

m. lemak 0,25 0,25 0,25 0,25 0,25

19,9 vol. titrasi 7,2 6,8 6,5 6,3 5,5

angka iod 62,54 64,51 65,98 66,97 70,91

III

m. lemak 0,25 0,25 0,25 0,25 0,25

19,9 vol. titrasi 7,2 7 6,6 6,2 5,8

angka iod 62,54 63,52 65,49 67,46 69,43

IV

m. lemak 0,25 0,25 0,25 0,25 0,25

19,9 vol. titrasi 7,3 6,8 6,5 6,2 5,8

angka iod 62,04 64,51 65,98 67,46 69,43

V

m. lemak 0,25 0,25 0,25 0,25 0,25

19,9 vol. titrasi 7,2 6,8 6,5 6,2 5,5

angka iod 62,54 64,51 65,98 67,46 70,91

Contoh perhitungan :

Angka iod =

Contoh pada ulangan I konsentrasi 0%:

Angka iod =

Lampiran 12. Standarisasi larutan Na2S203.5H2O 0,1 M

Cr2O72-

+ 14 H+ + 6 I

- 2 Cr

3+ + 3 I2 + 7 H2O

Cr2O72-

= 10 ml x 0,015 M = 0,15 mmol

I2 = 3 x 0,15 = 0,45 mmol

I2 + S2O32-

2 I- + S4O6

2-

I2 = 0,45 mmol

S2O32-

= 2 x 0,45 mmol = 0,9 mmol

Volume I II III

Volume awal 0 9,3 18,5

Volume akhir 9,3 18,5 27,8

Volume titrasi 9,3 9,2 9,3

Volume titrasi rata-rata = 9,27 ml

[S2O32-

] = 0,9 mmol / 9,27 ml = 0,097 M

Lampiran 13. Foto Tempe

1. Tempe dengan penambahan tepung belut 0% (kontrol)

2. Tempe dengan penambahan tepung belut 0,75%



5. Tempe dengan penambahan tepung belut 3%

18 wahyuningsih, r. 2011. tempe, superfood yang...

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