7/31/2019 17.Antiacne Activity of the Cream

1/1

Journal of Thai Traditional & Alternative Medicine Vol. 6 No. 2 May-August (Supplement) 2008

Antiacne activity of the cream containing aromatic volatile oil from

Thai Lanna medicinal plants entrapped in niosomes

Aranya Manosroi1,2

, Charinya Chankhampan1, Pisit Jainonthee

2, Jiradej Manosroi

1,2

1Faculty of Pharmacy, Chiang Mai University, Chiang Mai, Thailand, 50200

2Natural Products Research and Development Center (NPRDC), Institute for Science and Technology Research and Development,

Chiang Mai University, Chiang Mai, Thailand, 50200

Rationale: The pathology of acne is complex, involving abnormal keratinization, hormonal function, immune

hypersensitivity and bacterial growth (such as Propionibacterium acnes). Recently, many reports have found that

aromatic volatile oils from Thai medicinal plants can inhibit P. acne with no irritation to skin. In this study, the

cream containing the selected aromatic volatile oil from Thai Lanna plants which have been preliminary tested to

have antibacterial activity by an agar disc diffusion method was developed and investigated for antiacne activity in

human volunteers.

Objective: The objective of this study was to investigate antiacne activity in human volunteers of the cream

containing aromatic volatile oil from Thai Lanna medicinal plants entrapped in niosomes.

Method: The five plants which have evidences of antiacne were selected from the Thai Lanna medicinal plant

recipies such as hairy basil oil (Ocimum americanaL.), lemon grass oil (Cymbopogon citratusStapf.), Kaffir lime

leaf oil (Citrus hystrixDC.), Holy basil oil (Ocimum sanctum L.), and sweet vasil oil (Ocimum basilicum L.). The

extracts were prepared. The cream containing the plant extracts entrapped in niosomes was developed and tested

for antiacne activity in ten volunteers of 2 males and 8 females. The cream was applied twice daily on the area

of the face having acnes. The treatment was performed for 6 weeks followed by a 2 week wash-out period. The

antiacne activity was evaluated by macrophotographs at day 1 (baseline) and every week. The numbers of open and

closed comedones were counted and evaluated on 3-point scale (0 = no lesions cm-2, 1 = 10 lesions cm-2). The inflammatory lesions and skin compatibility of the products

focusing on redness, dryness, oiliness, peeling and itching were assessed according to the following scale: 0 = not

present, 1 = mild, 2 = moderate and 3 = severe.

Results: The average decreasing inflammatory lesion scales from baseline to week 6 (0, 1, 2, 3, 4, 5, 6 weeks)

were 2.1 1.10, 0.6 0.70, 0.5 0.85, 0.5 1.08, 0.3 0.67, 0.4 0.70 and 0.4 0.97 respectively, whereas

no significant change in the number of open and closed comedones (p value = 0.05) was observed. Skin

compatibility showed only minor oilness and no differences in the appearance of peeling and itching. This study

has confirmed the potential of the Thai Lanna medicinal plants when applied with nanotechnology for antiacne

activity.

Conclusion: The developed cream containing the Thai Lanna extracts entrapped in niosomes showed the antiacne

activity by reducing the inflamed lesions. This study has demonstrated the potential of the selected Thai Lanna

plants for cosmeceutical application.

PO-33