17933 revg 7-2012 useguide food fsd bd - bio- · pdf fileiso/ts 16649-3 (09/2005) horizontal...

Guide for Bio-Rad Products in Food Testing

July 2012

Food Safety

Bio-Rad Laboratories • Food Science

PART 1 – Routine Testing

Preparation of dilutions 3

Total flora 3

Escherichia coli – ISO standards 4

Escherichia coli – Alternative method: RAPID’E.coli 2 Agar 5

3

Coliforms – ISO standards 6

Coliforms – Alternative method: RAPID’E.coli 2 Agar 7

Enterobacteriaceae 8

Mesophilic lactic acid bacteria 9

ContentsGuide for Bio-Rad Products in Food Testing

Food Safety

This booklet is only an indication provided by Bio-Rad for the use of Bio-Rad products.

It is not a contractual document.

PART 2 – Pathogen Testing

Salmonella – ISO standard 10

Salmonella – Alternative method: RAPID’Salmonella Agar 12

Salmonella – Alternative method:iQ-Check™ Salmonella II real-time PCR Kit 13

Cronobacter spp. – ISO standard 14

Cronobacter spp. – Alternative method: RAPID’Sakazakii Agar 15

Cronobacter spp. – Alternative method:iQ-Check™ Cronobacter spp. real-time PCR Kit 15

Listeria monocytogenes – ISO standards 16

Listeria monocytogenes – Alternative method:RAPID’L.mono Agar 18

Listeria monocytogenes – Alternative method:iQ-Check™ Listeria monocytogenes II real-time PCR Kit 19

Listeria spp. – Alternative method:RAPID’Listeria spp. Agar 20

10

Listeria spp. – Alternative method: RAPID’L.mono Agar 21

Listeria spp. – Alternative method:iQ-Check™ Listeria spp. real-time PCR Kit 21

Escherichia coli O157:H7 – Alternative method:RAPID’E.coli O157:H7 Agar 22

Escherichia coli O157:H7 – Alternative method:iQ-Check™ E.coli O157:H7 real-time PCR Kit 23

Campylobacter spp. – Alternative method:iQ-Check™ Campylobacter real-time PCR Kit 23

Staphylococcus aureus – ISO standards 24

Staphylococcus aureus – Alternative method:RAPID’Staph Agar 25

Sulfite-Reducing bacteria 26

Clostridium perfringens 26

Bacillus cereus 27

Shigella 27

Routine Testing

Preparation of dilutions Total flora

NF EN ISO 6887-1 (09/1999)

Preparation of test samples, initial

suspension and decimal dilutions

for microbiological examination

Part 1: General rules for preparationof dilutions for microbiological

examination

NF EN ISO 4833 (05/2003)

General guidance for the

enumeration of micro-organisms

Colony-count technique at 30°C

Dilution 10-1

(and following dilutions)

m g or v ml of sample

+

9 x m g or 9 x v ml of

Tryptone Salt or Buffered Peptone Water

(and following dilutions)

Enumeration

PCA

Initial suspension

and/or

decimal dilutionsOrdering Information

Catalog # Description

PCA (Plate Count Agar)/Agar

Ready to use

355-4459 100 ml x 6 bottles355-4457 200 ml x 6 bottles356-3989 90 mm x 20 dishesDehydrated

356-4475 500 g

Tryptone-Salt/Diluent

Ready to use

355-5754 9 ml x 25 tubes355-5756 90 ml x 6 bottles355-5791 2.3 L x 5 bagsDehydrated

356-4544 500 g

Buffered Peptone Water/Broth

Ready to use

355-4179 225 ml x 6 bottles355-5789 2.3 L x 5 bags355-5790 5 L x 2 bagsDehydrated

356-4684 500 g

30°C72 hrs

Guide for Bio-Rad Products in Food Testing | | 3

ISO 16649-1 (04/2001)

Horizontal method for the enumeration

of -glucuronidase-positive E. coli

Part 1: Colony-count technique at 44°C using membranes and5-bromo-4-chloro-3-indolyl-

-D-glucuronide

TBX

Direct enumeration

Sample/

initial suspension

MMGA with membrane

ISO 16649-2 (04/2001)



Part 2: Colony-count technique at44°C using 5-bromo-4-chloro-

3-indolyl- -D-glucuronide

Escherichia coli - ISO standards

Dilutions

Resuscitation

Direct enumeration

Sample/

initial suspension

Dilutions

TBX

Ordering Information


TBX/Agar

Ready to use

355-5309 100 ml x 6 bottlesDehydrated

356-4035 500 g

Peptone Water/Broth

Ready to use

355-4180 9 ml x 25 tubesDehydrated

356-4334 500 g

Tryptose Lauryl Sulfate/Broth

Dehydrated

356-4774 500 g

EC Medium/Broth

Dehydrated

356-4764 500 g

RAPID’E.coli 2/Agar

Ready to use

355-5297 200 ml x 6 bottles355-5299 100 ml x 6 bottlesDehydrated

356-4024 500 g

44°C18-24 hrs

Room t° 15 minand 37°C 4 hrs ± 1 hr

44°C18-24 hrs

* Please refer to ISO 7218: 2007

(2 X)*

4 | | Guide for Bio-Rad Products in Food Testing

ISO 7251 (07/2005)

General guidance for detection and

enumeration

of presumptive Escherichia coli

Most probable number technique

ISO/TS 16649-3 (09/2005)



Part 3: Most probable numbertechnique using 5-bromo-4-chloro-3-

indolyl- -D-glucuronide

Enumeration of Escherichia coli

NF VALIDATION according to ISO 16140N° BRD 07/1-07/93 and BRD 07/7-12/04

AOAC-RI approved N° 050601 NordVal approved

All food stuffs

Sample/

initial suspension

RAPID’E.coli 2

Dilutions

Tryptose LaurylSulfate

EC Medium

Peptone Water

Indole test reading and calculation

Sample/

initial suspension

Single

concentration

Double

concentration

Routine Testing

37°C24-48 hrs ± 2 hrs

37°C24-48 hrs ± 2 hrs

44°C24-48 hrs± 2 hrs

44°C/37°C18-24 hrs

Escherichia coli Alternative method: RAPID’E.coli 2 Agar

Direct enumeration

of typical colonies*

* Enumeration of typical colonies

for E. coli at 44°C; for E. coli and

coliforms at 37°C

Sample/initial suspension

* For detection method only

(or)*

44°C48 hrs± 2 hrs

Each tube showing the presence of blue

colonies on TBX

should be considered positive

Calculate the most probable number

from the number of positive tubes

at each dilution

44°C20-24 hrs

37°C24 hrs ± 2 hrs

Add 10 ml

in 3 tubes of double

strength MMG

And subsequent dilution

Add 1 ml in 3 tubes

of single strength MMG

Subculture each tube presenting

yellow coloration on a dish of TBX


ISO 4831 (08/2006)

Microbiology of food and animal feeding stuffs

Horizontal method for the detection

and enumeration of coliforms

MPN technique

Sample

Tryptose Lauryl Sulfate

Coliforms - ISO standards

Dilutions



BGBLB (Brilliant Green BileLactose Broth)/Broth

Ready to use

357-8024 10 ml x 25 tubes

VRBL (Violet Red BileLactose)/Agar

Ready to use


356-4594 500 g

Tryptose Lauryl Sulfate/Broth

Dehydrated

356-4774 500 g

RAPID’E.coli 2/Agar

Ready to use

355-5297 200 ml x 6 bottles355-5299 100 ml x 6 bottlesDehydrated

356-4024 500 g

30°C or 37°C24-48 hrs ± 2 hrs

30°C or 37°C24 hrs ± 2 hrs

30-37°C24 hrs (+24 hrs)

Single

concentration

Double

concentration

Reading (gas +)

BGBLB

(or)*



Routine Testing

ISO 4832 (02/2006)

Microbiology of food and animal

feeding stuffs

Horizontal method

for the enumeration of coliforms

Colony-count technique

ColiformsAlternative method: RAPID’E.coli 2 Agar

Enumeration of coliforms

NF VALIDATION according to ISO 16140N° BRD 07/8-12/04

AOAC-RI approved N° 050601NordVal approved

All food stuffs

Direct enumeration

of typical colonies*

Sample/

initial suspension

RAPID’E.coli 2

Dilutions

Enumeration

of characteristic colonies

and confirmation

for atypical colonies

Sample/

initial suspension

Dilutions

* Enumeration of typical colonies

for E. coli at 44°C; for E. coli and

coliforms at 37°C

VRBL

30-37°C24 hr ± 2 hrs

37°C18-24 hrs

Coliforms

• Diameter ≥ 0.5 mm

• Purple with or without

a red zone of precipitated bile


(2 X)*


ISO 21528-1 (08/2004)


Horizontal methods for the detection

and enumeration of Enterobacteriaceae

Part 1: Detection and enumeration by MPN technique with pre-enrichment

VRBG

Oxidase Dextrose BCP

Yellow colour (+)

EE

Biochemical confirmation

Confirmation

Nutrient (2.8%)

Sample

Non selective

Pre-enrichment

Selective

enrichment

Buffered Peptone Water

Enterobacteriaceae

37°C24 hrs ± 2 hrs

37°C24 hrs ± 2 hrs

37°C24 hrs ± 2 hrs

37°C24 hrs ± 2 hrs

37°C18 hrs ± 2 hrs



EE/Broth

Dehydrated

356-4794 500 g

VRBG (Violet Red Bile Glucose)/Agar

Ready to use


356-4584 500 g

Nutrient (2.8%)/Agar

Ready to use


356-4484 500 g

Oxidase Test

355-3834 2 x vials of 50 oxidase disks


Ready to use


356-4684 500 g


ISO 21528-2 (08/2004)

Microbiology of food and animal feeding

stuffs

Horizontal methods for the detection

and enumeration of Enterobacteriaceae

Part 2: Colony-count technique

Routine Testing

Reading

Dilutions

Oxidase (-) Dextrose BCP

yellow colour (+)

Biochemical confirmation

Confirmation

Nutrient (2.8%)


VRBG

37°C24 hrs ± 2 hrs

37°C24 hrs ± 2 hrs

37°C24 hrs ± 2 hrs

(2 X)*


ISO 15214 (08/1998)

Microbiology of food and animal feeding

stuffs

Horizontal method for enumeration

of Mesophilic Lactic Acid Bacteria



Mesophilic Lactic Acid Bacteria

30°C72 hrs

Enumeration

MRS

(2 X)*




MRS (Man Rogosa and Sharp)/Agar

Dehydrated

356-4244 500 g


EN ISO 6579/A1 (02/2006)


Horizontal method for the detection of Salmonella spp.

Amendment 1 Appendix D: Detection of Salmonella spp. in animal faeces and in samplesfrom primary production stage

Salmonella - ISO standard

37°C ± 1°C24 hrs ± 3 hrs

37°C ± 1°C24 hrs ± 3 hrs

37°C ± 1°C24 hrs ± 3 hrs

41.5°C ± 1°C24 hrs ± 3 hrs

37°C ± 1°C18 hrs ± 2 hrs

RappaportVassiliadis Soya


MKTTn

Appendix D Protocol

Biochemical and serological confirmation

TSI Agar and other test

Plating-out

Confirmation

Nutrient 2.1%

Sample

Non selective

pre-enrichment

Selective

enrichment

Confirmation

Selective

isolation

XLD RAPID’Salmonellaor XLT 4

or Kristensenor Hektoen

MSRV

41.5°C ± 1.5°C24 hrs ± 3 hrs(+24 hrs for negative)


Pathogen Testing




Ready to use


356-4684 500 g

RVS (Rappaport Vassiliadis Soya)/Broth

Ready to use


356-4324 500 g

MKTTN (Muller Kauffmann Tetrathionate Novobiocin)/Broth

Ready to use

355-6140 10 ml x 50 tubesDehydrated Base

356-4714 500 gNovobiocin supplement

356-4610 1 vial TSI (Triple Sugar Iron)/Agar

Dehydrated

356-4384 500 g


Dehydrated

356-4485 500 g

XLD (Xylose Lysine Desoxycholate)/Agar

Ready to use

354-1751 90 mm x 20 dishesDehydrated356-9124 500 g

Selenite-Cystine/Broth

Complete medium

355-5744 20 ml x 25 tubes

MSRV (Semi-Solid Rappaport Vassiliadis Medium)

Ready to use


356-4325 500 g

XLT 4 (Xylose Lysine Tergitol 4)/Agar

Ready to use

356-3654 90 mm x 20 dishes

Brilliant Green (Kristensen)/Agar

Dehydrated

356-4464 500 g

Hektoen/Agar

Ready to use

355-4386 100 ml x 6 bottles356-3894 90 mm x 20 dishesDehydrated356-4284 500 g

RAPID’Salmonella/Agar

Ready to use

356-3961 90 mm x 20 dishes356-3962 90 mm x 100 dishesDehydrated

356-4705 500 g

Oxidase Test


Antiserum SalmonellaOmnivalent OMNI-O (A60)

356-0781 3 ml dropper bottle

Salmonella Latex Kit

Detection of groups B to E and G

355-6710 75 tests

Salmonella spp. Latex Kit

355-6711 50 tests




Antiserum SalmonellaOmnivalent OMNI-O (A60)

356-0781 3 ml dropper bottle

iQ-CheckTM Salmonella II Kit

357-8123 96 tests

ONPG Test

355-3822 Set of 50 disks

Oxidase Test


RAPID’Salmonella/Agar

Ready to use

356-3961 90 mm x 20 dishes356-3962 90 mm x 100 dishesDehydrated

356-4705 500 g

RVS/Broth (Rappaport Vassiliadis Soy)

Ready to use


356-4324 500 g

Salmonella Latex Kit

Detection of groups B to E and G

355-6710 75 tests

Salmonella spp. Latex Kit

355-6711 50 tests

RAPID´Salmonella Capsules /Supplement

356-4709 100 x Quantity for 2.5 l 356-4710 100 x Quantity for 250 ml356-4712 1 x Quantity for 100 analyses


Ready to use


356-4684 500 g

Salmonella - Alternative method: RAPID’Salmonella Agardouble enrichment protocol

37°C18 hrs± 2 hrs

41.5°C6-26 hrs

37°C24 hrs± 2 hrs

BufferedPeptone Water

RappaportVassiliadis Soya

RAPID’Salmonella

Sample

Plating-out

Reading & confirmation

Standard tests or Oxidase/OMNI-O/ONPG

Non selective

pre-enrichment

Selective

enrichment

Selective

isolation

Detection of Salmonella spp.


AOAC-RI approved N° 0507011

All food stuffs and feed


For more information on Bio-Rad’s real-time PCR kits and instruments, please contact your local Bio-Rad sales representative.1 - For AOAC-RI protocol, please contact your local Bio-Rad sales representative2 - 8-hr enrichment protocol available for raw meat (AOAC and AFNOR validated)

Pathogen Testing

Salmonella - Alternative method: RAPID’Salmonella Agarshort protocol

Salmonella - Alternative method: iQ-Check™ Salmonella II real-time PCR Kit

37°CFrom 10 hrs ± 2 hrsto 21 hrs ± 1 hr

41.5°C ± 1°C18 hrs± 2 hrs

37°C ± 1°C24 hrs± 2 hrs

Buffered Peptone Water+

Salmonella Capsule Supplement

RAPID’Salmonella

Sample

Plating-out

Reading & confirmation

Salmonella Latex or Oxidase/OMNI-O/ONPGor iQ-Check™ Salmonella


Sample

Selective

enrichment

Selective

isolation


Certification by NF VALIDATION according to ISO 16140 N° 07/11-12/05



AOAC-RI approved N° 010803NordVal approved N° 038

All foods stuffs1,2, feed, and environment including

primary production

Bacterial Lysis & DNA Extraction

(Standard or Easy)

Real-time PCR amplification and detection

with iQ-Check™ Salmonella II

Automatic result analysis and interpretation

Confirmation of positive with RAPID’Salmonella

Purchase of iQ-Check Kits includes an immunity from suit under patents specified in the product insert to use only the amount purchasedsolely in food testing, environmental testing, industrial microbiology and also for the purchaser’s own internal research. No other patent rights (such as 5’ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

ater

e


�+/-

ISO/TS 22964:2006 Milk and milk products -

Detection of Enterobacter sakazakii

Cronobacter spp. - ISO standard

37°C18 hrs ± 2 hrs

44°C24 hrs ± 2 hrs

44°C24 hrs ± 2 hrs

25°C48 hrs

Streak onto RAPID’Sakazakii

Perform identification

tests on yellow colonies

Sample preparationWeigh z g of the test portion

in 9 x z ml BPW

Selective enrichment

Transfer 0.1 ml in 10 ml

of mLST/vancomycin medium

Transfer 1 to 5 colonies on

TSA



RAPID’Sakazakii/Agar

Dehydrated

356-4976 500 g

Trypto-Casein-Soy (TSA)/Agar

Ready to use

356-3884 90 mm x 20 dishes 355-5947 200 ml x 6 bottles 355-5944 8 ml x 25 inclined tubes Buffered Peptone Water/Broth

Ready to use


356-4684 500 g iQ-CheckTM Cronobacter spp.

357-8137 96 tests


Pathogen Testing

RAPID’Sakazakii short protocol


Detection of Cronobacter spp. Infant Formulas and environment

NF VALIDATION pending

Cronobacter spp. - Alternative method: RAPID’Sakazakii Agarshort protocol

Cronobacter spp. - Alternative method:iQ-Check™ Cronobacter spp. real-time PCR Kit

37°C18 hrs ± 2 hrs

44°C24 hrs ± 2 hrs

25°C48-72 hrs

Streak onto RAPID’Sakazakii

Perform identification

tests on yellow colonies

Sample preparationWeigh z g of the test portion

in 9 x z ml BPW

Transfer 1 to 5 colonies on

TSA



(Standard or Easy)


with iQ-Check™ Cronobacter spp.


Confirmation of positive with RAPID’Sakazakii

37°C18 hrs ± 2 hrs


Environmental

samples

37°C4 hrs ± 1 hr

37°C20 hrs ± 2 hrs


Suppl. Buffered Peptone Water

Infant formulas

�+/-

EN ISO 11290-1/A1 (02/2005)


and enumeration of Listeria monocytogenes

Part 1: Detection method

Reading andconfirmation

Isolation

Primary enrichment

Isolation Secondary enrichment

Sample

Fraser ½

Fraser 1(0.1 ml culture/10 ml)

Listeria spp.

- TSYEA agar

- Catalase +

- Gram

- Mobility

Listeriamonocytogenes

- TSYEB

- Hemolysis

- Carbohydrates

- Camp test

Agar Listeria accordingto Ottaviani and Agosti

Other solid selective medium

Listeria monocytogenes - ISO standards

30°C24 hrs ± 2 hrs

35°C or 37°C48 hrs ± 2 hrs

37°C ± 1°C24 hrs ± 3 hrs(+24 hrs ± 3 hrs)

30°C-35°C-37°C(dependingof the media)24-48 hrs ± 2 hrs

Reading andconfirmation

37°C ± 1°C24 hrs ± 3 hrs(+24 hrs ± 3 hrs)

30°C-35°C-37°C(dependingof the media)24-48 hrs ± 2 hrs

Agar Listeria according

to Ottaviani and Agosti

Other solid selective medium


EN ISO 11290-2/A1 (02/2005)


and enumeration of Listeria monocytogenes

Part 2: Enumeration method

Listeria spp.

- TSYEA agar

- Catalase +

- Gram

- Mobility

Listeriamonocytogenes

- TSYEB

- Hemolysis

- Carbohydrates

- Camp test

Pathogen Testing

Enumeration and confirmation tests

Sample

Fraser 1/2 without LiCl

and selective supplement

or Buffered Peptone Water

Agar Listeria accordingto Ottaviani and Agosti

Dilution

20°C ± 2°C1 hr ± 5 min

37°C24 hrs ± 3 hrs(+ 24 hrs ± 3 hrs)




Ready to use


356-4684 500 g

Fraser ½/Broth

Complete medium

355-5797 225 ml x 6 bottles355-5788 2.3 L x 5 bagsBase

356-4604 500 gSupplement

356-4616 10 vials

Fraser 1/Broth

Complete medium

355-4569 10 ml x 25 tubesBase


356-4615 10 vials

AL (Agar Listeria accordingto Ottaviani and Agosti)/Agar

Ready to use

356-3695 90 mm x 20 dishes356-3696 90 mm x 100 dishesBase

356-4043 500 gBase in vials (to be supplemented)

355-5200 237.5 ml x 6 bottlesSupplement 1

356-4201 100 capsules (1 capsule qsp 250 ml)356-4041 10 vialsSupplement 2

356-4042 10 vials

Palcam/Agar

Ready to use

356-3674 90 mm x 20 dishesBase


356-4752 10 vials

Oxford/Agar

Ready to use

356-3664 90 mm x 20 dishes


Detection of Listeria monocytogenes


AOAC-RI approved N° 030406* NordVal approved

All food stuffs and environment

Listeria monocytogenes - Alternative method: RAPID’L.mono Agar

30°C24 hrs± 2 hrs

37°C24 hrs± 2 hrs

Isolation on

RAPID’L.mono

Reading and

confirmation tests

Fraser ½ Broth

Sample

Standard Tests

- TSYEB

- Hemolysis

- Carbohydrates

- Camp test

iQ-Check™

Listeria monocytogenes II

Spot on

Agar Listeria

according to

Ottaviani and

Agosti

Rhamnose Testor oror



RAPID’L.mono/Agar

Ready to use

356-3693 90 mm x 100 dishes356-3694 90 mm x 20 dishes355-5294 Kit: 190 ml agar + 2 suppl.Base

356-4293 500 gSupplement 1

356-4294 10 vialsSupplement 2

356-4746 10 vials


Ready to use


356-4684 500 gFraser ½/Broth

Complete medium



356-4616 10 vialsAL (Agar Listeria accordingto Ottaviani and Agosti)/Agar

Ready to use

356-3695 90 mm x 20 dishes356-3696 90 mm x 100 dishesBase

356-4043 500 gBase in vials (to be supplemented)

355-5200 237.5 ml x 6 bottlesSupplement 1

356-4041 10 vials356-4201 100 capsules (1 capsule qsp 250 ml)Supplement 2

356-4042 10 vialsLSB (Listeria Special Broth)

Ready to use

355-5703 225 ml x 6 bottles355-5793 5 L x 2 bagsDehydrated

356-4703 500 g356-4753 5 kgiQ-CheckTM Listeria monocytogenes II Kit

357-8124 96 tests

Rhamnose Test

355-3669 28 tests

For more information on Bio-Rad’s real-time PCR kits and instruments, please contact your local Bio-Rad sales representative.*For AOAC-RI protocol, please contact your local Bio-Rad sales representative


Pathogen Testing

37°C24 hrs± 2 hrs

Isolation on

RAPID’L.mono

Direct

enumeration


Sample

Enumeration of Listeria monocytogenes



20°C1 hr

Resuscitation

For a NF VALIDATION validated

enumeration method, in the event

of a positive result using

RAPID’L.mono for enumeration,

confirmation is not necessary when

the presence of Listeria monocytogenes

has been confirmed during detection.

Listeria monocytogenes - Alternative method: iQ-Check™ Listeria monocytogenes II real-time PCR Kit

30°C23 hrs ± 1 hr (1)

or 25 hrs ± 1 hr (2)

(1) Listeria Special Broth (LSB) or (2) Fraser ½ Broth

Sample

Detection of Listeria monocytogenes


AOAC-RI approved N° 010802NordVal approved N° 037



(Standard or Easy)

Real-time PCR amplification and detection with

iQ-Check™ Listeria monocytogenes II


Confirmation of positive with RAPID’L.mono

Purchase of iQ-Check Kits includes an immunity from suit under patents specified in the product insert to use only the amount purchased solely in food testing, environmental testing, industrial microbiology and also for the pur-chaser’s own internal research. No other patent rights (such as 5’ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contac-ting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.


�+/-

Reading and confirmation tests

Isolation on RAPID’Listeria spp.

Sample

Fraser ½ Broth

Standard

Tests

- Gram

- Catalase

Spot on

RAPID’L.mono+ Gram test

+ Catalase

Spot

on PalcamiQ-Check™

Listeria spp.

30°C24 hrs ± 2 hrs

37°C24 hrs ± 2 hrs



RAPID’Listeria spp./Agar

Ready to use

356-3950 20 x 90 mm dishesBase

356-4744 500 gSupplement 1

356-4745 10 vials Supplement 2

356-4746 10 vials

Fraser ½/Broth

Ready to use



356-4616 10 vials

Palcam/Agar

Ready to use



356-4752 10 vials

LSB (Listeria Special Broth)

Ready to use

355-5703 225 ml x 6 bottles355-5793 5 L x 2 bagsDehydrated

356-4703 500 g356-4753 5 kg

iQ-CheckTM Listeria spp. Kit

357-8113 96 tests

For more information on Bio-Rad’s real-time PCR kits and instruments, please contact your local Bio-Rad sales representative.

*For AOAC-RI protocol, please contact your local Bio-Rad sales representative

Listeria spp. - Alternative method:RAPID’Listeria spp. Agar

Detection of Listeria spp.


AOAC-RI approved N° 080701*All food stuffs and environment

or or or


Pathogen Testing

Listeria spp. - Alternative method:iQ-Check™ Listeria spp. real-time PCR Kit



AOAC-RI approved N° 090701*Health Canada Validation -MFLP-39



or




30°C24 hrs± 2 hrs

37°C24-48 hrs

Isolation on

RAPID’L.mono


Fraser ½ Broth

Sample

Standard Tests

- TSYEA

- Gram

- Catalase

iQ-Check™

Listeria spp.

Listeria spp. - Alternative method: RAPID’L.mono Agar


30°C23 hrs ± 1 hr (1)

or 25 hrs ± 1 hr (2)

Listeria Special Broth (LSB)

Sample

Bacterial Lysis & DNA Extraction ((1) Standard or (2) Easy)

Real-time PCR amplification and detection with

iQ-Check™ Listeria spp.


Confirmation of positive with RAPID’Listeria spp. or RAPID’L.mono

�+/-



RAPID’E.coli O157:H7/Agar

Base

356-4748 100 g

mTSB/Broth

Ready to use

(containing novobiocin)

355-5426 225 ml x 6 bottlesBase

356-4426 500 g

Novobiocin

Dehydrated

356-4610 1 g


Ready to use


356-4684 500 g

iQ-CheckTM E.coli O157:H7 Kit

357-8114 96 tests

iQ-CheckTM Campylobacter Kit

357-8135 96 tests

For more information on Bio-Rad’s real-time PCR kits and instruments, please contact your local Bio-Rad sales representative.

*For AOAC-RI protocol, please contact your local Bio-Rad sales representative

Escherichia coli O157:H7 - Alternative method: RAPID’E.coli O157:H7 Agar

41.5°C16-24 hrs

mTSB + Novobiocin

Immuno-magnetic-separation

Sample

37°C24 hrs ± 2 hrs


Standard tests or Latex or

iQ-Check™ E.coli O157:H7

Isolation on RAPID’E.coli O157:H7

Detection of Escherichia coli O157:H7


AOAC-RI approved N° 060701*All food stuffs and environment

RAPID’E.coli O157:H7 can also be used as a second medium

in the ISO 16654 standard


Pathogen Testing

Escherichia coli O157:H7 - Alternative method: iQ-Check™ E.coli O157:H7 real-time PCR Kit

Campylobacter spp. - Alternative method: iQ-Check™ Campylobacter real-time PCR Kit

41.5°C8-24 hrs

41.5°C20 hrs

37°C 4 hrs

42°C 24 hrs


Suppl. Bolton Broth 2X BF-BEB

Sample Food Sample Carcass rinse or sponge

Detection of Escherichia coli O157:H7

NF VALIDATION according to ISO 16140 N° BRD 07/15-06/08 Raw beef

AOAC-RI approved N° 020801*Raw ground beef, fresh spinash, apple cider

Detection of Campylobacter jejuni, Campylobacter coli

and Campylobacter lari

AOAC-RI approved N° 031209*

Food samples, carcass rinses or sponges and feces samples

Bacterial Lysis &

Easy DNA Extraction

Bacterial Lysis and DNA Extraction

(Easy)


with iQ-Check™ E.coli O157:H7


with iQ-Check™ Campylobacter

Result analysis and interpretation Result analysis and interpretation

Confirmation of posiitve with RAPID’E.coli O157:H7



+

�+/- �+/-

NF EN ISO 6888-1/A1 (01/2004)

Horizontal method for the enumeration of

coagulase-positive Staphylococci

(Staphylococcus aureus and other species)

Technique including confirmation of colonies

Staphylococcus aureus - ISO standards

35°C or 37°C24 hrs ± 2 hrs(+ 24 hrs ± 2 hrs)

35°C or 37°C24 hrs ± 2 hrs

35°C or 37°C4-6 hrs or 24 hrs

Confirmation

Brain-Heart Infusion

Rabbit Plasma

Coagulation reading

Sample/initial suspensionand following dilution

Enumeration

Typical Staphylococci

Grey to black colonies with a clear halo around

and a white opaque zone



Baird-Parker/Agar

Complete medium


356-4814 500 g

Baird-Parker for RPF/Agar

Complete medium

357-8618 90 ml x 6 bottles + 6 vials of suppl.356-3996 90 mm x 20 dishesBase

356-4814 500 g

Additives

Egg yolk with potassium tellurite

355-4201 5 ml x 1 vial355-4205 25 ml x 1 bottleSulfamethazine solution (0.2%)

356-2682 2.5 ml x 1 vial

PastorexTM Staph Plus Latex

355-6356 50 tests355-6353 5 x 50 tests

RPF Supplement

356-4618 10 vials

BHI (Brain-Heart Infusion)/Broth

Ready to use


356-4014 500 g

Rabbit Plasma

Freeze-dried

355-6352 Pack for 20 reactionsSpecial broth

355-3544 9 ml x 25 tubes

RAPID’Staph/Agar

Ready to use


356-4704 500 g

Baird-Parker

(2 X)*



NF EN ISO 6888-2/A1 (12/2003)


of coagulase-positive Staphylococci

(Staphylococcus aureus and species)

Technique using Rabbit plasma Fibrinogen agar medium

NF EN ISO 6888-3 (06/2003)


of coagulase-positive Staphylococci

(Staphylococcus aureus and species)

Detection and MPN techniquefor low numbers

35°C or 37°C18-24 hrsand 48 hrs

37°C24 hrs ± 2 hrs (+24 hrs ± 2 hrs)

37°C24-48 hrs

37°C4-6 hrsor 24 hrs

37°C24 hrs

37°C24-48 hrs

Direct enumeration of typical

coagulase-positive staphylococci

Sample/initial suspensionand following dilution

Rabbit Plasma

Confirmation of

typical colonies

on BHI

Reading

Direct

enumeration

of typical

coagulase-

positive colonies


Giolitti and CantoniBroth

Simple concentration

Baird-Parker Baird-Parkerfor RPF

+ RPF sup.

37°C24 hrs ± 2 hrs

RAPID’Staph


Enumeration and Confirmation

Staphylococcus aureusAlternative method:RAPID’Staph Agar

Baird-Parkerfor RPF

+ RPF sup.

or

Gioletti and CantoniBroth

Double concentration(or)*


Enumeration of Coagulase-positive

Staphylococci


AOAC-RI approved N° 080602*All food stuffs, feed and environment

Pathogen Testing

(2 X)*


PastorexTM

Staph Plus

Spot on

Baird-Parker

for RPFor

* For AOAC-RI protocol, please contact your local Bio-Rad sales representative


ISO 15213 (05/2003)

Microbiology of food and

animal feeding stuffs

Horizontal method for

the enumeration of sulfite-reducing

bacteria growing

under anaerobic conditions

Reading

Food sample/initial suspension

Dilution

Confirmation

NF EN ISO 7937 (02/2005)


of Clostridium perfringens

Colony-count technique

Sulfite-reducing bacteria Clostridium perfringens

Thermophilous

bacteria

suspicion

50°C 24-48 hrs

Anaerobes

condition

LactoseSulfite



TSC without D-Cycloserine/Agar - Base Medium

Ready to use

355-4419 100 ml x 6 bottlesBase

356-9644 500 g

Lactose Sulfite/Broth

Base

356-4914 100 g

Thioglycolate + Resazurin/Broth

Ready to use


356-4084 500 g

Sheep Blood/Agar

Base

356-4674 500 gReady to use

356-3784 90 mm x 20 dishesSheep Blood Supplement

355-6652 50 ml vial

37°C24-48 hrsanaerobescondition

Heat treatment 20 min 75°C

37°C20 hrs ± 2 hrs

37°C18-24 hrs

37°C18-24 hrs

46°C24 hrs

Enumeration

of typical black colonies

Confirmation

of 5 isolated colonies with

Thioglycolate + Resazurin


MobilityNitrate

LactoseGelatine

Clostridiumperfringens

(yellow/gas+)


(red coloration/

gas+)


(black precipi-

tate/gas+)

Anaerobes

condition

TSC

TSC (add D-Cycloserine)

(2 X)*


(2 X)*



Bacillus cereus

NF EN ISO 7932 (07/2005)

General guidance for the enumeration

of Bacillus cereus


Confirmation test

on 5 well isolated colonies


Pathogen Testing

30°C24 hrs ± 2 hrs

30°C18-24 hrs(+24 hrs)

Sheep Blood/Agar

Reading and interpretation



Mossel/Agar (Mannitol Egg Yolk Polymixin)

Dehydrated

356-9604 500 g

XLD (Xylose Lysine Desoxycholate)/Agar

Ready to use

354-1751 90 mm x 20 dishesDehydrated

356-9124 500 g

Mac Conkey + Crystal Violet/Agar

Ready to use

356-3617 90 mm x 20 dishesDehydrated

356-9084 500 g

Hektoen/Agar

Ready to use

355-4386 100 ml x 6 bottles356-3894 90 mm x 20 dishesDehydrated

356-4284 500 g


Dehydrated

356-4485 500 g

Novobiocin

Dehydrated

356-4610 1 g

Mossel (MYP)

Shigella

NF EN ISO 21567 (03/2005)

Horizontal method

for the detection of Shigella spp.

37°C20-24 hrs(+48 hrs)

37°C20-24 hrs

41°C16-20 hrs

Plating-out

Shigella Broth + NovobiocinPH 7 ± 0.2

Spot on typical colonies on

Nutrient (2.1%)


Biochemical and serologicalconfirmation

MacConkey

XLD Hektoen

(2 X)*



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Website www.bio-rad.com/diagnostics U.S. 1-800-2BIO-RAD Australia 61-2-9914-2800 Austria 43-1-877-8901 Belgium 32-9-385-5511 Brazil 5521-3237-9400

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