Cell Culture studiesCell Culture studies

-We have previously shown SPIO particles are taken up avidly by macrophages. Our cell culture studies showed a transient elevation in free radical of macrophages with a rapid return to the baseline.

-Next we tested a new SPIO, (lipid coated) versus the old (dextran coated).

Cell Culture StudiesCell Culture StudiesWe have Comparison of the 3 different SPIOs in the cell culture, and their effect on macrophage biology,.

Preliminary results indicated that there is asignificant difference between the level of NOproduction from macrophages, when they engulf dextran coated SPIO versus lipid coated (Feridex is the commercially available SPIO)

Induction of NO in Induction of NO in Macrophages by Different Macrophages by Different

forms of SPIOforms of SPIOInduction of Nitirc Oxide by SPIO and LPIO-particles in

Macrophages -24 h

CNS-1 2d CNS-1 df CNS 5M 1bCNS 5M 2bCNS 5 ML 2cCNS 5 ML 1-6Feridex Control0.0

0.1

0.2

0.3

CNS-12dCNS-1dfCNS 5M 1bCNS 5M 2bCNS 5 ML 2cCNS 5 ML 1-6FeridexControl

SPIO or LPIO particles

OD5

50 (N

itrite

leve

l)

SPIO

LPIO

ResultsResults

 

 Unlike dextran coated SPIO which are taken up by mannose receptors, lipid coated SPIO enters into macrophages through scavenger receptors and induce less oxidative stress.

Atherosclerotic Rabbit Aorta, x10 magnifiedH&E and Iron – SPIO loaded cells shown in blue

Atherosclerotic Rabbit Aorta, x10 magnifiedH&E and Iron – SPIO loaded cells shown in blue

Blue cells