Microsoft PowerPoint - 1040915 -1040908 [] Other lab workers Cleaners


Risk Group


1 No or very low No or very low
Risk Group
3 High Low Usually available
4 Hi h Hi h U ll N


y
6


--6--
Bacillus licheniformis, Escherichia coli- K12 Ad d



y
7


--7--
41
Outline

M b l i B kh ld i d ll i B kh ld i ll i



Histoplasma capsulatum, Coccidioides immitis, Blastomyces dermatitidis


y
0 prions9


p SARS-CoV, Polio virus (all types) TSE (Transmissible spongiform encephalopathy), HIV, HTLV,
--9--
SARS CoV MERS CoVSARS-CoV, MERS-CoV Outline



RG3: H5N1H7N9




0
Ebola virus, Marburg virus, Lassa virus, Crimean-Congo hemorrhagic fever virus hemorrhagic fever agents and


y
hemorrhagic fever virus, …, hemorrhagic fever agents and viruses as yet undefined
19


--19--
EbolaEbola “Outbreak” movieOutline


(RG2)



y
fever virus, Hantavirus, Chikungunya virus, Inf A H5N1Inf A H5N121


: Ebola virus, Marburg virus, Lassa virus, Crimean-Congo hemorrhagic fever virus hemorrhagic fever agents and
--21--
Risk Group Risk Group-
Outline



Outline



BSL 1BSL-1


y
34


--34--
Matthew G. et al. ABSL-4 Aerobiology Biosafety and Technology at the NIH/NIAID Integrated Research Facility at Fort Detrick hViruses 2014, 6(1), 137-150;
-

A ( )





--37--

-20130723.pdf
Outline






Traps particulates onlyOutline




y
42


--42--
https://zh.wikipedia.org/wiki/%E9%AB%98%E6%95%88%E6%BB%A4%E7%BD%91
2 2 2.2
--43--
70% of air recirc lated ithin the cabinet Outline
70% of air recirculated within the cabinet 30% exhausted through the HEPA filter into the room
Type A2 (B3)
yp ( ) Same as A1 except air is exhausted outside through a
thimble unit connection. Type B1

BioSafety Level Type B1
30% of air recirculated 70% exhausted through the HEPA filter to the outside via a


y
No recirculation with total exhaust through the HEPA filter to44


No recirculation with total exhaust through the HEPA filter to the outside via a hard duct
--44--
Class III BSC (BSL 4 Lab)Class III BSC (BSL-4 Lab) Outline



Sash-Sash











S l h/ ill


Well balanced




y
60


--60--

Outline





y
61


--61--

Working Outside Normal HoursOutline o g Ou s de o a ou s
/PI


/PI
Risk Group


y
PPE




1513