1

One-Minute NMR• History• Current Events

17 August 2006

Presented by Tim Peck &

Dean Olson

2

e-Seminar Etiquette

Press *6 to mute

Press #6 to un-mute

(to ask a question)

As a courtesy to others, please mute your phone line while listening:

3

One-Minute NMRhistorical roots

UIUC Campus

Paul Lauterbur: MRI

Herb Gutowski Memorial

4

One-Minute NMR2006 tour

• Am. Soc. of Pharmacognosy: Arlington, VA 8/6 – 8/9• ENC: Monterey, CA 4/24 – 4/28

5

MicroFlow NMRThe NMR Front End for the Modern Analytical Laboratory

6

Live ParticipationEnter Your Sample Info

7

System OperationWatch the Analysis!

8

NMR Data Collected Pre-Conference Varian Inova 600 MHz

9

View the Results!Automated Structure Confirmation

Click

…

Thumbs Up!(Confirmation)

Thumbs Down!(Rejection)

10

View the Results!Automated Structure Confirmation

Integrated ACD/Web Librarian

11

Am. Soc. PharmacognosyAugust 6-9, 2006

12

Natural ProductsChemical Assignment Rapid Dereplication

M. Gronquist, J. Meinwald, F. C. Schroeder, JACS 2005, 127, pp. 10810-10811

dqf-COSY spectrum 8 scans 512 complex incrementstotal time 3 h 45 min

coupled non-gradient HMQC spectrum 16 scans, 512 complex incrementstotal time 7 h

non-gradient HMBC spectrum baseline-subtracted in F164 scans, 460 complex incrementstotal time 21 h 45 min

NOESY spectrum 16 scans, 256 complex incrementstotal time 4 h 18 min

dqf-COSY

HMQC

HMBC

NOESY

S. Van der Sar, J. Blunt, and M. Munro, Org. Let. 2006, 8(10), pp. 2059-2061

13

Some time for fun…

14

And re-connecting with old friends

15

How Small is 10 μL?

16

Autosampler Injection Port

17

Autosampler Needle on a96-Well Microtitre Plate

18

Needle at Wash Station

19

Autosampler Control Pad

20

Solvent Conditioner Bottles

21

Solvent Conditioner

22

One-Minute NMR Features

SampleReturn

SampleReturn

StackedSample

Drawers

23

One-Minute NMRSystem Configuration

24

25

Quiz Question: What is this?

26

Hint:Am. Soc. Pharmacognosy Dinner Cruise was down the Potomac

river