1 - dna and rna
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B.Lewin Genes
J.Watson Molecular biology of the geneB.Alberts Molecular biology of the cellH.Lodish Molecular cell biology
(nucleobases)
PyrimidinePurineAdenineGuanineThymineCytosine
- (ATP)
( ) (.. ).
12345
++--
15432
: (), (), (G) (C)
AdenineGuanineThymine : A, C, G, T. 5- 3-. () (sugar backbone).53
Francis Crick & James Watson
Francis Crick (1916-2004)
James Watson
,
(1951) A + G = C + T , A = T; C = G
-
-
- - (Watson-Crick base-pairs)
2,8 3,0 2,9 3,0 2,9
11,1 10,8
(double helix) ,
A-DNAB-DNAZ-DNA
- =
,
Hershey-Chase , 32 35S , - .
! , , 2-3 . : 4 . : 1-70 ??
, , . 147 .
- - - 30 -
Fluorescence in situ hybridisation (FISH) ,
DNA vs. RNA (5-)2-- ! m5C, m6A ! mRNA, rRNA, tRNA, snRNA, snoRNA, miRNA, ,
tRNA
http://prion.bchs.uh.edu/bp_type/bp_structure.html
Triplexes : rRNA tRNA ( )
G - Quadruplexes - . ( )