1 development a portable device for multi-channel impedance measurement in dielectrophoresis-chip...
TRANSCRIPT
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Development a Portable Device for Multi-Channel Impedance Measurement
in Dielectrophoresis-chip
Chairman : Dr. Hung-Chi Yang
Presenter : Ping-Yang Liao
Adviser : Dr. Yi-Chun Du
Date : 2014/11/19
南台科技大學奈米科技研究中心
Integrated Biomedical System Laboratory
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Outline
• Introduction • Purposes• Material and Methods• Results• Future Works• References
南台科技大學奈米科技研究中心
Integrated Biomedical System Laboratory
Program Outline
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Integrated Immunoassay Chip
System based on Impendence Sensing
and DEP Manipulation of
Nanoprobes
Detection chip and
Fluid
Signal analyze
Biological material
Introduction
According to the statistics from Department of Health, disease diagnosis nephritis, nephrotic syndrome and nephrosis has been ranked in the 8th or 10th among the top 10 causes of death from 2002 to 2008.
5http://cetd.tmu.edu.tw/etdservice/view_metadata?etdun=U0007-0907201012073900
Introduction
End stage renal disease (ESRD) incidence and prevalence in Taiwan are both 1st in the world. The number of dialysis patients is increasing every year in Taiwan. This issue causes enormous health care spending and has become the top expenditures in the record of National Health Insurance.
6http://cetd.tmu.edu.tw/etdservice/view_metadata?etdun=U0007-0907201012073900
Background
• Kidney damage will effect albumin increased.
• “Microalbuminuria”is important to index.
(<300mg/L)• Clinic detecting
– Semi-quantitative method – Urine test for 24 hour
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Aging Society
• Local hospital or home – Inexpensive– Short time measurement– Easy ues– Early invent and early treat
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Handhold System Design
• Higher Noise Tolerance (SNR ↑)• High detection range and multi-channel
design • Automatically analysis• Lower Power Consumptions (Battery)
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Dielectrophoresis force(DEP)
• Dielectrophoresis is a phenomenon in which a force is exerted on a dielectric particle when it is subjected to a non-uniform electric field .
• All particles exhibit dielectrophoretic activity in the presence of electric fields.
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Dielectrophoresis force(DEP)
• The strength of the force depends strongly on the medium and particles' electrical properties, on the particles' shape and size, as well as on the frequency of the electric field.
• Fields of a particular frequency can manipulate particles with great selectivity.
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Direct Digital Synthesis(DDS)
• Direct Digital Synthesizer (DDS) is a type of frequency synthesizer used for creating arbitrary waveforms from a single, fixed-frequency reference clock.
• Frequency 1KHz~1MHz
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Voltage Control Current Source( VCCS)
• The source delivers the current as per the voltage of the dependent element.
• I = fb(Vx)
• Range 1KHz~1MHz
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Results
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Resistance(kΩ)Our system
measured( kΩ)|Error|(%)
100 103.102 3.105
220 228.61 3.913
330 320.779 2.794
490 476.755 2.703
680 688.812 1.295
820 831.42 1.392
1000 956.11 4.389
Capacitor(pF)Our system
measured(pF)|Error|(%)
1 0.959 4.129
4.7 4.877 3.775
10 10.366 3.658
22 22.276 1.256
33 32.481 1.574
47 48.124 2.391
68 65.506 3.667
References
• S. Kun, B. Ristic, RA. Peura, RM. Dunn, “Real-time extraction of tissue impedance model parameters for electrical impedance spectrometer,” Med. Biol. Eng. Comput., vol. 37, pp. 428-432, 1999.
• J. G. Webster, Electrical Impedance Tomography, Adam Hilger, IOP, New York, USA, 1990.• F. Seoane, J. Ferreira, J. J. Sanch´ez and R. Brag´os, “An analog front-end enables electrical
impedance spectroscopy system on-chip for biomedical applications,” Physiol. Meas., vol. 29, pp. S267–S278, 2008.
• D. C. Walker, B. H. Brown, R. H. Smallwood, D. R. Hose and D. M. Jones, “ Modelled current distribution in cervical squamous tissue,” Physiol. Meas., vol. 23, pp. 159–168, 2002.
• R. H. Smallwood, A. Keshtkar, B. A. Wilkinson, J. A. Lee, and F. C. Hamdy, “Electrical impedance spectroscopy (EIS) in the urinary bladder the effect of inflammation and edema on identification of malignancy,” IEEE Trans. Med. Imaging, vol. 21, pp. 708–710, 2002.
• C. A. Gonz´alez-Correa, B. H. Brown, R. H. Smallwood, D. C. Walker and K. D. Bardhan, ”Electrical bioimpedance readings increase with higher pressure applied to the measuring probe,” Physiol. Meas., vol. 26, pp. S39–S47, 2005.
• J. L. Damez, S. Clerjon, S. Abouelkaram, J. Lepetit, “Dielectric behavior of beef meat in the 1–1500 kHz range: Simulation with the Fricke/Cole–Cole model,” Meat Sci., vol. 77, pp. 512-519, 2007.
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