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UijtU15f11i

Faculty of Veterinary Medicine Khan Kaen University

123 Mitraparb Rd Amphur ~uang Khon Kaen 40002 THAILAND

TelFax +66 (43) 202404

letter of Confirmation

February 28 th 2012

Dear Dr Naruepon Kampa

I hereby confirm that your abstract Study of gastro-intestinal transit time in rabbit using

barium sulphate for poster presentation is submitted and is under consideration by the Scientific

Committee of the 13th KVAC June 7th_8th

2012 Khon Kaen Thailand

We are looking forward to meeting you in Khan Kaen

Best regards

AssistProfDr Jaruwan Kampa

Chairman Scientific Committee

Faculty of Veterinary Medicine Khan Kaen UniveJsity

123 Mitraparb Rd Amphur lVIuangI

Khan Kaen 40002 THAI~ND TeLFax +66 (43) 202 404

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Letter of Confirmation

February 28th 2012

Dear Dr Chaiwat Jarassaeng

I hereby confirm that your abstract The effect of Corynebacterium cutis lysate (Ultra-cornregj

to control somatic cell count in dairy cows for poster presentation is submitted and is under

consideration by the Scientific Committee of the 13th KVAC June 7th_8th 2012 Khan Kaen Thailand


Best regards

- Z IrVJfrlt -L

I



13th KV AC International conference on Trans-boundary Disease Impact on ASEAN One Health

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IPresentation Type Oral Poster J

Session Emerging amp Re-emerging disease J Livestock

Small animal I

Wild-life Exotic and Aquatic Keywords Subclinical mastitis Somatic cell count Corynebacteriam cutis I IDairy cows Publication as full Yes I will submit this research in full-paper format by 25 May 2012 I

---I paper in KKU Vet No I would like to present the research data as abstract in the conference J proceeding only

The effect of Corynebacterium cutis lysate (Ultra-corn~ to control somatic cell count in dairy cows

Kanograt Suchalerm1 Chadaporn Sirirungsakulwong( Suriya Lamsida(Chaiwat Jarassaengzt

lThe 6th student of Veterinary Medicine Faculty ofVeterinary Medicine KhonKaen University 2Department of Surgery and Theriogenology Faculty ofVeterinary Medicine KhonKaen University Corresponding author

Abstract

The aim of this study is to evaluate the effectiveness of repeated inoculations of a Corynebacterium cutis lysate (Ultra-Cornreg) in commercial dairy farm at Phimai district

Nakhonratchcasima province by randomly selected 20 dairy cows with SCC over 250000 cellsm Cows were divided randomly into two groups also found Streptococus uberis infected in 7 cows Treated group received 3 weekly 20 mgIOO kg ofUltra-Cornreg injected intra-musculary for 3

weeks while the control group received distilled water instead Milk was collected 1 week before

injection then followed entire period for 8 weeks of observation and collected milk for analysed for

SCC by using Fossomaticreg The result was showed no significant differences between the

treatment and control groups of cows In this study the immuno-stimulant effect of Ultra-Comreg a

Corynebacterium cutis lysate could not be decreased SCC in infection ofStreptococcous uberis

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DCnClc Penetration Of Salmonella Through Eggshell Sunpetcll Angkititrakuf Arunee Popakdet Pitloya Chaiamnai Yupadee KOlilmet Vilhiril1ee Srikenkhar J) Khon Kaen University Research And Diagnostic Center For Emerging Infectious Diseases 2) Faculty OJ Veterinary Medicine Khon Kaen University

This study was to determine the ability of Salmonella to penetrate chicken eggshelL A total of 63 chicken eggs divided into 3 groups of 21 eggs were used for each of 2 replications The control eggs were wiped with 70 alcohol and kept at room temperature Eggs in the treatment groups were dipped in Salmonella Enteritidis 108 cellml solution The first treatment group was kept in a refrigerator (8-lOoC) and the other group was kept at room temperature (25-30 oC) One egg from each group was examined for Salmonella at the outer and inner shell white egg and yolk every day for 21 days No Salmonella were found in the control group Salmonella were identified from outer shell inner shell white egg and yolk of refrigerated eggs at 10023848 and 48 respectively Detection of Salmonella from outer shell inner shell white egg and yolk samples of eggs kept at room temperature was at 667 286 48 and 48 respectively The study showed that the storage method and temperature influenced the survival and penetration ability of Salmonella Refrigeration promoted the survival of Salmonella at outer shell however inhibited the bacterium to penetrate the inner shell better than the storage at room temperature

DCnC2b Survival Rate Among HivAids Hilltribe Marginalized Populeion In Northern Thailand

Tawatchai Apidechkul School aHealth Science Mae Fa Luang University

The retrospective cohort study des~gn aimed to studies of current situation ofHIVAlDS sexual behaviors and survival time in the Isix main hilltrib peoples in l11ailand Akha Lahu Itarean Mong Yao and Lisaw where the ~ost ep~demic of HI-VIAIDS areas in Thailand The first step was a systematic data extraction ofmedidal records of hiUtribe mYAIDS cases fr~m 37 hospitals distributed in 4 provinces innorthern of Thailand followed by fact-to-fact interview ~ith the valid and reliable questionnaires Statistic analyses were contributed by Survival Analysis arid CoxsshyRegression Of 581 from 608 subje~ts were included in the analysis 810 of suBjects -Were alive 396 were aged 26-35 years at th~1 diagn~sed date 649 was female 362 was Lahti 298 was Karean 577 were Buddhist and 216 ~ere Christian 575 were infected by sexual inteI]course 62 IDU 50 ofsurvival time of receijed ARV was 1239 years whereas 599 yeyens for the non-ARV group (p-valueOO 1) 50 of survival time of nonshyOI infection was 1059 years whereas 633 years for the OI group (p-valueOOl)i 50 sfrvival time of female was 664 years and 1239 years in male (pshyvalueOOl) Coxs-regressior model found that female (HR 055 95CI 0Of-082)~ receiving ARV (HR 043 95CI 027~O69) and non-01 (HR 053 95CI 001-089) wer~ factor~ favoring good survival time The specific m~thods for given knowledge ofHIVAlDS suc4 as peer education in marginalized are necessary a$ well as encourage them to use condom

DCnC2c I

Evaluation Of Tuberculosisi Contr~l By DOTS Strategy In Phonthong Hos~ital~oi-Et Province Supai Buranavech Wanpen iPhoonperm Phonthong Hospital

The objective of this retrospective sthdy was to evaluate and curative perfofnyentce of hew smear positive Pulmonary cases usi~g DOlTS strategy in Phonthong Hospital during October 2004shySeptember 2006 Samples wete new smear positive Pulmonary Tuberculosis case~ who Were treated with CAT 1 (2HRZE4HR) a~d usediDOTS strategy It was found that thesputu~ conversion rate was 94S9cure ratedeath ratefailure rate and

I I default rate was 9279631 dego090ro and 000 respectivelyThe conversion rate as well as cure rate

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Activity of Eucalyptus (Eucalyptus melliodora) Wood Vinegar Against Cattle Ticks

(Boophilus spp)

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Abstract

Activity of Eucalyptus wood vinegar (Eucalyptus melliodora) against cattle tick (Boophilus

spp) was investigated in 25 native cattle The skin of cattle were marked and count the ticks before

spraying with 50 (vv) Eucalyptus wood vinegar solution Then the ticks in the same area were

counted at 12 and 24 hours The results showed that the mean number of tick before spraying was

289plusmn4 (xplusmnSD) and the mean number of tick after spraying at 12 and 24 hours were 17plusmnS (xplusmnSD) and

14plusmn5 (xplusmnSD) respectively The study concluded that 50 Eucalyptus wood vinegar solution had

statistically significant reduced the ticks at 12 and 24 hours (pltOOS) However the duration of action

concentrations and types of wood vinegar nay be effective against cattle ticks differently

Keyword Eucalyptus wood vinegar and cattle ticks

Email ppittakkuacth

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2un~mtliltl~ 6 P1rulaquo[iJlLLYIMJ911tllilf lJtlliVltrl~tJJJflwLLriw 40002

1 Department of Pathobiology Faculty of Veterinary Medicine Khon Kaen University 40002

th 2 6 Year Student Faculty of Veterinary Medicine Khon Kaen University 40002

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1untJmiddotnWnl1t11lIwlll1n11 l-Jfl lritl rl11Vl2 WV11lVIm~tJiI1I1W9J1WVIflVlmL1I(iJnLri(iJ mnlPl q I

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Bastos RG Ueti M W Knowles D P and Scoles G A 2010 The Rhipicephals (Boophils)

micripus BM 86 gene play s a critical role in the fitness of ticks fed on cattle during acute

Babesia bovis infection Parasites amp vectors3 111

Bowman DD 2009 Georgis Parasitology for Veterinarians 9th edition Suanders Elevier Inc

Missouri USA

Borges LM Ferri P H Silva W J Silva WC and Silva JG 2003 In vitro efficancy ofextracts

Melia azedarach against the tick Boophilus micropius Medical and Veterinary EntermoJogy 17(2)

228-23)

Chungsamamyrart N Jiwajinda S Rattanakreetakul C and Jansawan W 1991 Practical extraction

of sugar apple seed against tropical cattle ticks Kasetsart J (Nat Sci suppl) 25 101-105

Chungsamamyart N and S Jiwajinda 1992 Acaricidal activity of volatile oil from lemon and citronella

grasses on tropical cattle ticks Kasetsart J (Nat Sci Suppl) 26 46-51

Chungsamamyart N and W Jansawan 1996 Acaricidal activity of peel oil of citrus spp on

Boophilus microplus Kasetsart J (Nat Sci) 30 112 -117

Cramer LGcarhalvo LAF Bridi A A Amaral NK and Barrick R A 1988 Efficacy oftropically

applied ivermectin against Boophilus microplus (Canestrini 1887) in cattle Veterinary Parasitology

29(4) 341-349

Futse1 E Ueti M W Knoeles DP and Palmer G H 2003 Transmission of Anaplasma

rnarginale by Boophilus miclOpius Retention of vector Competence in the Absence of Vectorshy

Pathogen Interaction Journal of Clinical Microbiology 41(8) 3829-3834

Miller RJ Davey R B and George lE 2005 First Report of Organophosphate-resistant Boophilus

micropius (AcariIlxodidae) within the United State Journal of Medical Entomology 42(5) 912-917

Mitsuyoshi Y Madoka N Keko H Tatsuro O and Akira S 2002 Termiticidal activity of wood

vinegar its components and their homologues Journal of Wood Science 48 338-342

MuhammadG Saqib M Athar M Khan M Z and Asi MN 1999 Clinico-Epidemiological and

Therapeutic Aspects of Bovine Thaileriosis Pakistan Vet J 19 (2) 64-71

Pegram RG and Lemche J 1985 Observation s on the efficacy ofivermectin in the control of cattle

ticks in Zambia Veterinary Record 117 551-554

Taylor MA Coop RL and Wall RL 2007 Veterinary Parasitology 3m

edition Blackwell Publishing

Ltd UK

Wen B Jian R Zhang Y and Chen R 2002 Simultaneous Detection ofAnaplasma marginale and a

New Ehrlichia Species Closely Relate ofEhrlichia chaJJeensis by sequence Analyses of 16S Ribisomal

DNA in Boophilus micriplus Ticks from Tibet Journal of Clinical Microbiology 40(9) 3286-3290

No JITMM201lfSC132

21 October 2011

AsslProf Sirikachom Tangkawattana Facu1ty ofVeterinary Medicine Khon Kaen University

Acceptance of abstract for poster presentation J1TMM 2011

Dear AsstProf Sirikaehom Tangkawattana

On behalf of the JITMM2011 Scientific Committee I am pleased to inform you that your abstract entitled ULTRASONOGRAPHY OF HEPATOBllIARY PATHOLOGY IN TIlE Opisthorchis viverrini INFECTED CATS has been accepted for poster presentation in the JITMM2011 1-2 December 2011 Bangkok Thailand

The rectangular poster-board surface area for each presenter is 90 cm wide x 180 em high An appropriate poster size therefore is 85 em wide x 150 cmhigh

AU poster presentation at JITMM2011 will be considered for the Poster Presentation Award Please visit our website httpwwwjitmmcom for more information

As the presenting author we thank you very much for your registration and payment If you have not already done so please complete your registration via our website httpwwwjitmmcom We recommend you to register by 31 October 2011 while the early bird registration rate is still applicable

Thank you very much for your interest in and submission to this Meeting

Looking forward to your presentation at the Meeting

Yours sincerely

w~~ AsstProf Pongrama Ramasoota Chairperson Scientific Committee JITMM2011 PhonelFax 66 (0) 2306 9125-6 E-mail iitmmmahidoLacth Website wwwjitmmcom

JITMM secretariat Faculty of Trlplcal Medicine Mahidol Unive~ity 4206 Ratchawlthl Road Bangkok 10400 Thailand Phone +662354 9100 ext 1524 1525 +6623049125 Fax +6623069125 +66 23069126 E-mail jitmmmahidolacth Website wwwjitmmcom

Comparing expression of HER-2 in Malignant Feline Mammary Tumors Using Tissue Microarray

N Rungrassamee2 P Tawornpanich2

W Jintawes2 S Balthaisong34 SWangnaithum5

P Tangkawattanal A RungsipipatS and S Tangkawattana24

IDept of Veterinary Anatomy 2Dept ofVeterinary Pathobiology Khon Kaen Univ Khon Kaen THAILAND

3Dept of Pathology Khon Kaen Univ Khon Kaen THAlLAND 4Tropical Disease Research Laboratory Khon Kaen Univ Khon Kaen THAILAND

5Dept of Veterinary Pathobiology Fac ofVeterinary Science Chulalongkorn Univ Bangkok HAILAND

Corresponding author email sirikachkkuacth

Keywords Feline mammary tumor Immunohistochemistry HrR2 Tissue microarray

Introduction HER-2 or c-erbB-2neu is a

transmembrane protein belonging to epidermal growth factor receptor (tyrosine kinase receptor) family Overexpression and amplification of HER-2 has been recognized in breast carcinogenesis for decades Although Ordas et a1 [1] proposed the feline mammary carcinoma to be a natural model of highly aggressive and honnone-independent breast cancer in human similar manner in animal tumors is still questioned This study aimed to study HER-2 expression in malignant feline mammary tumors by developing tissue microarray (TMA) technique

Materials and Methods Tumor tissue microarray construction

Seventy feline mammary carcinomas (FMC) were collected from the Department of Pathobiology Faculty of Veterinary Science Chulalongkorn University during 2005-2009 and routinely processed for HampE slides Histological grading was based on WHO tumor classification for feline mammary tumors [2J Sites for TMA were screened and marked on HampE slide and donor block TMA blocks were manually constructed with TMA Eazy a mod(tieo module of Dr- Bah~hob SripaoLKKU Tissuecorewas15 mm in diameter Each sample was performed triplicately

Immunohistochemistry The ABC or Benchmark XT (Roche Diagnostics Switzerland) immunohistochemical staining was performed on the tissue microarray

Primary antibodies used in this study wen monoclonal rabbit or mouse anti-HER-2 (PATHWAY HER-2neureg clone 4BS for Benchmark XT Roche Roche Diagnostics Switzerland) and monoclonal mouse antishyPCNA antibody (Cell Signaling USA for ABC) Human breast cancer and tonsil were used as positive controls respectively

Evaluation Percentage and grade of the tumor cells having positive PCNA and HER-2 expression were rep(rted Statistical analysis was perfonned by one-way analysis of variance CANOVA)middot and chi- square or Fishers exact test Statistical significance was considered at the level of 95 confidence

Results All FMCs were proven to be epithelial

type in origin bymiddot ur colleagues (Dr A Rungsipipat) Majority of FMCs was the simple tubular adenocarcinoma (TAC=5571 3970) Other FMCs were papillary adenocarcinoma (PAC 1000 770) cribrifonn carcinoma (CC= 1429 1070) and solid carcinoma (SC=2000 1470) PCNA index of TAC PA CC and SC were 4792plusmn1685 4226plusmn1161 3627plusmn1O62 and 4700plusmn1846 respectively

Expression of HER-2 was observed in either membrarlus or cytoplasmic staining This was shown only in mammary carcinoma cells Only 9 of 73 (1233) FMCs were positive to HER-2 The percentage of HER-2 expression categorized by histological pattern was 1765 (634) in TAC 1429 (17) in PA 10 (110) in CC and 1429 (2114) in

The Joint Meeting of Conference and Congress of Asian Society of Veterinary Pathology (ASVP) 2011 amp The 10th Scientific Symposium oflndonesian Society of Veterinary Pathology (ISVP) 2011

SC respectively However there was no statistically significant relationship between the HER-2 expression and histological grades

Discussion TMA is a highly throughput technology

that make it possible to collect a large number of archived paraffin embedding tissue at timely and economical cost Even this technique is widely used in human cancer research there are a few publications involving TMA in veterinary medicine The incidence of HER-2 overexpression in FMCs ranged from 36-90 [1 3 4 5] Similar to previous report of Taweechart et at [6] revealedmiddot overexpression of HER2 in 4167 ofFMCs Altematively our study reflects low frequency of HER-2 overexpression (1233) similar to the study by Rasotto et aL [7] Like in human breast cancer this probably involves in different phenotypes having different invasive and metastatic behaviors [7] Another possibility is antibody specificity to the binding site Primary antibody in our study is specific to internal domain of HER2 In conclusion HER-2 overexpression may not play a crucial role in feline mammary carcinogenesis Acknowledgements

We would like to thank Assoc Prof Banchob Sripa Experimental Pathology Unit Department of Pathology Faculty of Medicine and Tropical Disease Research Laboratory Khon Kaen University for his kind support of tissue microarrays equipment This project was partially supported by the Grant for Special Problem of the Faculty of Veterinary Medicine Khon Kaen University

References 1 Ordas et al 2007 Proto-oncogene HER-2

in normal dysplastic and tumorous feline mammary glands an imnwnohistochemical and chromogenic in sttu hybridization study BMC Cimcer 7179 Doi 1011861471-2407-7-179

2 Hampe and Misdorp 1~74 Tumofu and dysplasias of the mammary gland In Bull World Health Organ 50(1-2)1-6

3 De Maria et al 2005 Spontaneous feline mammary carcinoma is a model of HER-2

overexpression poor prognosis human breast cancer Cancer Res 65907-12

4 Millanta et al 2005 Overexpression of HER-2 m feline mvaSlve mammary carcinomas an immunohistochemical survey and evaluation of its prognostic potential Vet Pathol42 30-34

5 Winston et al 2005 Immunohistochemical detectionmiddot of HER-2neu expression in spontaneous feline mammary tumours Vet Comp OncoI38-15

6 Taweechart et al 2004 The expression of c-ERBB2 oncogene protein as a prognostic factor in feline mammart tumors Thai J Vet Med 34 76-90

7 Rasotto et at 2011 An Immunohistochemical study of HER-2 expression in feline mammary tumours J CompPath 144 170-7~

Figure 1 HER-2 immunohistochemistry using tissue microarray A) TMA slides (HampE IRC) B) Representative cores C) D) Membranous positive staining of FMC and human breast cancer as a positive control (HER-2 x400) original magnification

amp0

~u 41

0 Jt21

bull Tnmiddotl1~(

PCNAmdC~

3D

20

10 ~

rAe PAC CC SC

Figure 2 HER-2 expression and PCNA index in various tumor types TAC=simple tubular adenocarcinoma PAC=papillary adenocarcinoma CC=cribriform carcinoma and SC solid carcinoma

I

Invited Speaker Page OPS I TGF-beta Niche Regulation for Better Therapy

jin-Kyu Park Eun-Mi Lee2 Ah-Young Kim12 Sang-Young Youi2 Seon-Young Han l1 Eun-Joo Leel2 and Kyu-Shik Jeongl1

OPS 2 Pathological findings and Molecular charcteristics ofPRRSV isolated from infected iii North of Vietnam Nguyen Thi Lani

Nguyen Huu Nami Nguyen Ba Hieni Le Huynh Thanh Phuoni Nguyen Thi Hoal Ryoji Yamaguchl

OPS 3 Rat Tumor Models and their Applications Particularly for Renal and Hepatic Fibrosis Jyoji Yamate

OPS 4 Vascular Pathology Associated with Experimental Pasteurella multocida serotype B2 Infection in Calves

80 Jasni EM Amna M Zamri-Saad Z Zakaria 80S Arshad AR Omar and T 1 Azmi

OPS 5 Challenges in safety evaluation ofbiomaterials biomedical devices and engineered tissue constructs TV Anikumar R I]epa and M Jaseer

OPS 6 Diagnostic Classification and Detection of Minimal Residual Disease (MRD) in Canine Lymphoma A Rungsipipat N Manachai J Chayapong S Wangnaitham and 80 Techangamsuwan

OPS 7 The Development ofPorcine Reproductive and Respiratory Syndrome Virus accine Min-Yuan CfiiaG

Hui-Ting Chanb Yi-Yin Dob

Pung-Ling HlIanl Victor Fei PangG Chian-Ren

Jenga

Participant POI Pathology and Veterinary Pathology Challenge-Future

Perspective 11 Al Sultan

P 02 Ultra structural Characteristic of Blood Cells in Giants Freshwater Stingray (Himantura chaophraya) ASailasuta Y Mathura N Chansue

P03 Analysis of P53 and PCNA Expression in the Genital and Extra genital CTVT Microarray S Jantautsa K Tangtragoon P Kholatha 80 Balthaisong S Wangnaithilm PTangkawattana A Rllngsipipat 80 Tangkawattana

P04 Comparing Expression ot HERR-2 in Malignant Feline Mammary Tumors using Tissue Microarray N Rungrassamee P Tawornpanich W Jintawes 80 BalthaisongS Wangnaithum P Tangkawattana A Rungsipipat S Tangkawattana

P 05 Investigation of the Death of Java Rhinoceros (Rhinoceros sondaicus) in Ujung Kulon National Park Adhi Rachmat Slldrajat Hariyadi Handayani Agus Priyambudi Ridwan Setiawan

P 06 Pathogenicity of a Pathogenic Filed Isolate NDV IBali-l07 in Commercial Chicken Strain ISA brown Anak Agung AYll Mirah Ad Made Kardena Nyoman Mantik Astawa Yoshihiro Hayashi Yaslll10bu Matsumoto

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Page 25

26

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Xl

Proceedings

The Joint Meeting of Conference and Congress of Asian Society of Veterinary Pathology

(ASVP) 2011 amp

The 10th Scientific Symposium of Indonesian Society of Veterinary Pathology (lSVP) 2011

The Role of Veterinary Pathology Animal Health for Improving Eco-Health

IPB International Convention Centre Bogor Indonesia November 2011

Hosted by Faculty of Veterinary Medicine Bogor Agricultural Jniversity 2011

CONFERENCE MANUAL amp PROCEEDINGS

S ConftzrStnelZ amp Congrua

ASyP 2~1 ASIAN SOCIETY OF VETERINARY PATHOLOCY

22 - 24 ltov~mb~r Sogor - 1ltf)oltelgtl~

The Joint Meeting of the 5th Conference and Congrelt

Asian Society ofVeterinary Pathology (ASVP) 2011 ~

The 10th Scientific Symposium of Indonesia Society of Veterinary Pathology (ISVP) 2011

Organized by

4VIJ

Asian Society of VeterInary Patht)logy

P03middot

Analysis of P53 and PCNA Expression in the Genital and Extragenital CTVT Microarray

S Jantautsa2 K Tangtragoon2

P Kholatha2 S Chanlun2

S Balthaisong34 SWangnaithum5 P

Tangkawattana A Rungsipipae and S Tangkawattana24

lDept of Veterinary Anatomy 2Dept of Veterinary Pathobiology Khon Kaen Univ Khon Kaen THAILAND 3Dept of Pathology Khon Kaen Univ Kpon Kaen THAILAND 4Tropical pisease Research Laboratory Khon Kaen Univ Khon Kaen THAILAND

5Dept ofVeterinary Pathology Fac of Veterinary Science Chulalongkorn Univ Bangkok THAILAND


Keywords immunohistochemistry P53 tissue microarray transmissible venereal tumor

Introduction USA) In addition human adenocarcinoma Mutation of tumor suppressor gene p53 is and tonsil were used as the positive control

frequently found in human cancer Although Evaluation Percentage of PCNA and the wild-type P53 protein is rapidly degraded number of P53 positive cells was reported and undetectable within the cell the mutantshy One-way analysis of variance (ANOV A) and type is stabilized and accumulated within the chi-square or Fishers exact test was nucleus of which is immunohistochemically performed and the statistical significance is detectable This mutant protein could alter the considered at the level of95 confidence cell cycle leading to neoplastic transformation Thus far the P53 abnormality Results has been described in various canine tumors The 55 CTVTs could be classified into 16 such (1S osteosarcoma mast cell tumor genital and 39 extragenital types Two of 16 lymphoma (1 2 3 4] However the reports in (125) genital CTVTs were positive to P53 nataral ~anine transmissible venereal tumor while 9 of 39 (2308) were found in the (CrVT) are very few This study aims to extragenital CTVTs Mean percentageplusmnSD of analyze expression of P53 in the micro array the P53 positive cells was 2660plusmn2408 in the ofCTVT genital group and 2492plusmn2969 in the

extragenital group MeansplusmnSDs of the PCNA Materials and Methods index was 1l78plusmn1307 in the genital group

C l1strllction of tumor microarray Fiftyshy middotand-2660plusmn2408 in the extragenital group five canine transmissible venereal tumors However there is no statistically different of (CrVTs) collected during 2009 from animal P53 expression and PCNA index between hospitals in Bangkok and Khon Kaen genital and extragenital groups (pgtO05) Thailand were routinely processed for HampE slides After the histopathological screening Discussion the tissue microarray (TMA) blocks were Tissue microarray is a powerful research manually constructed with TMA Eazy a tools for investigating animal tumors The P53 modified module of Dr Banchob Sripa of gene has been implicated in various biological Khon Kean University Thailand The tissue processes including DNA repair cell-cycle core was 15 mm in diameter Each sample arrest apoptosis autophagy senescence and

-

WS performed triplicately~ _metabolism In our study a low frequency of Immunohistochemistry Primary antiboshy P53 expression was demonstrated in both

dies used in this study were monoclonal genital and extragenital crvrs The two mouse anti-P53 antibody (PATHWAY P53reg times higher in the expression or P53 and clone D07 for Benchmark XT Roche Roche PCNA index in the extragenital CTVTs than Diagnostics Switzerland) and monoclonal those in the genital group would possibly mouse anti-PCNA antibody (Cell Signaling reflect a higher genetic alteration during the

The Joint Meeting of Conference and Congress of Asian Society of Veterinary Pathology (ASVP) 2011 amp The 10th Scientific Symposium ofIndonesian Society of Veterinary Pathology (ISVP) 2011

tumorigenesis Interestingly most CTVTs 3 Jaffe et al 2000 Immunohistochemical showed cytoplasmic or ectopic P53 Cellular and clinical evaluation of p53 in canine localization of P53 is often found in loss of cutaneous mast cell tumors Vet Pathol 37 wild-type P53 activity as well as protein 40-46 nucleocytoplasmic shuttling [5 In 4 Sueiro et al 2004 Canine lymphomas A conclusion the p53 expression would not play morphological and immunohistochemical a major role in CTVT carcinogenesis and study of 55 cases with observations on p53 alteration of P53 often presents in extragenital immunoexpression J Comp Pathol

c-middottlvT This is need to study further tOdlt 13h207-213~ p i-

understand its mechanism 5 OBrate and Giannakakou 2003 The importance of p53 location nuclear or

Acknowledgements cytoplasmic zip code Drug Resistance We would like to thank Assoc Prof Update 6 313-22

Banchob Sripa Experimental Pathology Unit 6 Department of Pathology Faculty of Medicine and Tropical Disease Research Laboratory Khon Kaen UniversityKhon Kaen Thailand for his kind support of tissue microarrays equipment and Vet Central Lab Nonthaburi for provide some specimens This project was partially supported by the Grant for the Special Problem of the Faculty of Veterinary Medicine Khon Kaen University Thailand

Figure 1 Immunohistochemistry of P53 in References the CTVT microarray A) TMA slides (HampE1 Sagartz et al 1996 P53 tumor suppressor IHq B) Representative cores C)

protein overexpression is osteogenic Cytoplasmic staining 0) Nuclear positive tumors of dogs Vet Pathol 33 213-21 staining in human adenocarcinoma (positive

2 Gamblin et al 1997 Overexpression of control) respectively (HER-2 x400)p53 tumor suppressor protein in original magnification spontaneously arising neoplasms of dogs Am J Vet Res 58 857-63

I

ntrll I ~il

_ ti

-lr r bull ~lt ~

29

Invited Speaker Page OPS 1

OPS2

OPS3

OPS4

OPSS

OPS6

OPS 7

POI

P02

P 03

P04

P 05

P 06

TGF-beta Niche Regulation for Better Therapy jin-Kyu Park Eun-Mi Leel2 Ah-Young Kim 12 Sang-Young Youl2 Seon-Young Han] Eun-Joo Leel2 and Kvu-Shik Jeong2

Pathological findings and Molecular charcteristics ofPRRSV isolated from infected in North of Vietnam Nguyen Thi Lan I Nguyen Huu Naml Ngu~en Ba Hien1 Le Huynh Thanh Phuont Nguyen Thi floaL Ryoji Yamaguchi] Rat Tumor Models and their Applications Particularly for Renal and Hepatic Fibrosis Jyoji Yamate Vascular Pathology Associated with Experimental Pasteurella multocida serotype B2 Infection in Calves

S Jasni EM Amna M Zamri-Saad Z Zakaria Ss Arshad AR Omar and T I Azmi Challenges in safety evaluation ofbiomaterials biomedical devices and engineered tissue constructs TV Anikumar R Deepa and M Jaseer Diagnostic Classification and Detection of Minimal Residual Disease (MRD) in Canine Lymphoma A Rungsipipat N Manachai J Chayapong S Wangnaitham and S Techangamsuwan The Development of Porcine Reproductive and Respiratory Syndrome Virus Vaccine

Min- Yuan Chiaa Hui- Ting Chanb Yi- Yin Dob

Pung-Ling Huanl Victor Fei PangCl Chian-Ren

Jenga

Participant Pathology and Veterinary Pathology Challenge-Future Perspective

Il Al Sultan Ultra structural Characteristic of Blood Cells in Giants Freshwater Stingray (Himantura chaophraya) ASailasuta Y Mathura N Chansue Analysis of P53 and PCNA Expression in the Genital and Extra genital CTVT Microarray S Jantautsa K Tangtragoon P Kholatha S Balthaisong S Wangnaithum P Tangkawattana A Rungsipipat S Tangkawattana Comparing Expression of HERR-2 in Malignant Feline Mammary Tumors using Tissue Microarray N Rungrassamee P Tawornpanich W Jintawes S Balthaisong S Wangnaithum P Tangkawattana A Rungsipipat S Tangkawattana Investigatfon of the Death of Java Rhinoceros (Rhinoceros sondaicus) in Ujung Kulon National Park Adhi Rachmat Sudrajat Hariyadi Handayani Agus Priyambudi Ridwan Setiawan Pathogenicity of a Pathogenic Filed Isolate NDVBali-I07 in Commercial Chicken Strain ISA brown Anak Agung Ayu Mirah Adi Made Kardena Nyoman Mantik Astawa Yoshihiro Hayashi Yasunobu Matsumoto

3

5

8

11

13

L5

20

Page 25

26

28

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Proceedings


(ASVP) 2011 amp


The Role of Veterinary Pathology Animal Health for Irnproving Eco-Health

IPB International Convention Centre Bogar Indonesia November 2011

Hosted by Faculty of Veterinary Medicine Bogar Agricultural University 2011


l Jmiddot~

1ASllP 2 ASIAN SOCIETY OF VETERINARY PATHOL04Y

22 - 24 iov~ber Sogor - IN~OietgtITf

The Joint Meeting of the 5th Conference and Congres Asian Society ofVeterinary Pathology (ASVP) 2011 lt1 The 1Qth Scientific Symposium of Indonesia Socie~ of Veterinary Pathology (ISVP) 2011 1r

Organized by

~VI)

Asian Society of ~terlnary Patho109YAPVI

ABSTRACTS

Poster No 07 ULTRASONOGRAPHY OF HEPATOBILIARY PATHOLOGY IN THE OPISTHORCHIS VIVERRINI INFECTED CATS

Thonwiwot KronS1Yllh Nattha Pothias Prapan Kanchamrao Prasarn Tangkowattonao Banchob Sripabc Sirikachorn Tongkawoltonagcd

bull Faculty of Velerinary lv1edicine Khan Koen University Khon Kaen 40002 Thaibnd Faculty of Medicine Khon Koen University Khan Koen 40002 Thailand Tropical Disease Research Lobor Khon Kaen University Khon Kaen 40002 Thailond

d liver Fluke and Cholal19iocarcinamo Research Cenfer Khan Kaen University Khon I(aen 40002 Thailand

Corresponding author emiddotmail sirikachkku actll

Objectives - To investigate the pathological

changes of the liver and biliary tract in

Opisthorchis viverrini (0 viverrini) infected cots

using ultrasonography

Methods - Infection and egg count per gram

(EPG) of 0 viverrini in 6 cats with natural infection was

determined by formalin ether concentration technique

Regards hepatobiliary images (thickness of gall bladder

and hyperechoicity of liver parenchyma) of these 6

infected cots in comparison with those of the other 4

healthy uninfected cats were examined by an ultrasound

equipment ILogic 5 Pro GE lechnology UK)

Results - All cots were at the mature age lat

leest 1 year) The EPG ranged widely from 25 to 3 144

with meanplusmnSD of 92883plusmn 1196Ll6Although hepatic

hyperechoicity was observable inall infected cots only

half of them (36) showed an increasing thickness of

gall bladder However these hepatobiliary changes

were not observable in all healthy uninfecled cats

Notably the pathologic changes increased in the heovy

and very heavy infected cats Presumably hepatobiliary

changing pattern as a result of 0 viverrini infection in

cat and human would be indifferent tilt

Keywords Opisthorchis viverrini Cat Ultrasonography

Acknowledgement This project was cb-supported by

KKU Research Grant 2010 and the Grant for the Special

Problem Projects of the FJculty of Veterinary Medicine

Khon Kaen University Thailand

Poster No 08 PREVALENCE OF OPISTHORCHIS VIVERRINI INFECTION IN BITHYNIA SNAILS IN OPISTHORCHIASIS ENDEMIC AREAS IN NORrHEAST THAILAND

I lt I i lt~

A~iporn SuwannatraiO Yuji Arimotsuo Cristina S KimbBanchob Sripao

o Tropical Disease Research Laboratory Deporlmenf of Pathology Faculty of Medicine Khan Kaen University Khon Kaen 40002 Thoiland Deportment of Environmenlol Health Science of Public Health The Ol1io Siole Ullversily Coillmbus 43201 USA

Objective - To sUNey current status of Opisthorchis viverrini infection in Bithynia snails collected

_ _from opisthorchiasis endemic areas in northeast

Thailand

Methods - Bithynio snails were collected from

25 water bodies in Khon Kaen Mahasorakham and

Kaiasin Provinces and were examined for trematode

infection by cercarial shedding method The cercaria

were identified by their morphological characteristics

external and internal organ under stereomlcroscope

confirmed by PCR

Results - -here were 8 types of cercaflae

infection in Bilhynia snails including 0 viverrini Virgulate

Amphistome Mutabile Longifurcate Xiphidio and 2

unknown cercariae types The prevalence of 0 viverrini infection in the Bithynio snails was 077 1476137)

which was found only in the snails collected from Chi

Kok Kor Village Lawa Lake Khon Keen Province PCR

analysis confirmed 0 viverrini specific amplification

The prevalence of Amphistome Mutabile

Longifurcate Xiphidio and 2 unknown cercariae was

213 023 003 008 007 007 and

009 respectivelybull

Keywords Opisthorchis vivernni Bithynia snails

Cercariae Prevalence PCR

List of Poster Presentations

t Poster No Page

ltspiniger(lmo[lligerrtyJmmun6blotiest detecling 24kD proteinmiddot

i) ~ -S ~ __1 -1 rj-q ~ t _U 1~ltgt~ ~ilt~_ gtlt ~Jgtlt~(~_ ~~4~-laquo 4~ _ _ ~ ~ lt~ ~~ 1 -~fn v lt _ ~ - - gt --iP-i -

Or A~SI~idi~r)~rhighse~u~middottbtor~lgEand~kint~5t~fgnatnostomiaSis using porfidlly purified specific q~dfh0sioma

Vala Bussbrond I bull bull bullbull c bullbull 44

8b~g(Rdbf~~~i humari middot~~tiix metalloprojease-9by secretory metdlloproteas~s from infective 510ge Angiosirongylus

middot G6rjt0I1~ehS~PbP~(~~JO~~ong 144

j)3MoIecular ideritificaliorifAnisaklslorvae jNematodd Anisokiddel isolated from marine fish off the Gulf of Thailand 1 PropbothipEamsobhano 145

middot04 The18S ribosomOlDNA sequence of Strongyloides stercoralis in Thailandmiddot Konifta Prolnmo 145

05 Anti-opoptotic activity of Opisthorchis viverrini excretory-secretory proteins aid its recombinant thioredoxin-l in a biliary cell line

Pitchoya Matchimakul 146

06 The endo~~~~ ~h~~~dfts~cret~~ ~~~~ct~f ~-ish~~~hs ~i~~~rl~l ~u~~ ~ff~ct to ~holan~oc~rclno~~ 146

97 Ultrasonography of hepatobiliary pathology in the Opisthorchis viverrini infecled cats Sirikcchom Tangkqwatlono 147

08 Prevalence ofOpsrhorchis yvefrinf infection in Bithynia snails In opisthorchiasis endernit-oreas in northeast Thailand Apipotn Suwannotrai 147

09 Population genetic analyses of Opisthorchis viverrini high concordance between microsatellite DNA and allozyme markers

Paiooon Sithithovvorn 148

10 Cytokine profiles in Opisfborchis viverrini stimuloted PBMCS from cholangiocarcinoma patients Arpa Surapairoon 148

11 Simple imrnunodiagnosis of opisthorchiaisis by detection of fecal parasite antigens using indirect ELISA Salma Teim0ori 149

12 Discovery of OpisthorcHis obaus ITrematoda Opisthorchiidael new recorded species of small liver flukes

in the Gr~~~~~~h~~~~~b-r~g~~ 149

13 Clonorchis sinensis an~genic proteihs for development of serologic rapid diagnostic kit Sung1ng H9ng 150 I

middot 14 Epidemiologyof Fasciola giga~tlfo and rumen flukes from Bubous bubois and 8o~jauruS regions Thailand

in northern and northeastern

Cb(JqbolY(ongsawadJ 150 I I

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17bull ~u~eyoF~hel~lQthslinmiddot~lli~ fres~water fishes from Kwal1 Phayao Thailand

k raong yutSrlpalwIf l 152

f~j middot1~ i~ i 18 yenltIu~~a~tlb~ heml~oI chartfterizotion of a novel cysiatin from the liver fluke Fasciola giganica

Smr~~~rIf0on i 152 9 MetQq~rcCiria1 irliJdlons onre~hv1dtet fishes at Ban Koksalung Posak Choldsid Dam Thailand

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Joint International Tropical Medicine Meeting 2011

(JITMM 2011)

IIOne World-One Health

1-2 December 2011

Centara Grand amp- Bangkok Convention Centre At Central World Bangkok Thailand

Organized by bull The Faculty ofTropical Medicine Mahidol University

SEAMEO TROPMED Network

bull The Parasitology and Tropical Medicine Association ofThaiiand

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Faculty of Veterinary Medicine Khan Kaen University

123 Mitraparb Rd Amphur ~uang Khon Kaen 40002 THAILAND

TelFax +66 (43) 202404

letter of Confirmation

February 28 th 2012

Dear Dr Naruepon Kampa

I hereby confirm that your abstract Study of gastro-intestinal transit time in rabbit using

barium sulphate for poster presentation is submitted and is under consideration by the Scientific

Committee of the 13th KVAC June 7th_8th

2012 Khon Kaen Thailand


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POI

P02

P 03

P04

P 05

P 06






Jenga



3

5

8

11

13

L5

20

Page 25

26

28

30

32

35

Xl

Proceedings


(ASVP) 2011 amp






l Jmiddot~




Organized by

~VI)


ABSTRACTS



































I lt I i lt~





Thailand







confirmed by PCR










213 023 003 008 007 007 and





t Poster No Page



















in the Gr~~~~~~h~~~~~b-r~g~~ 149













(JITMM 2011)


1-2 December 2011


















l1UU~fI1j7jli1 fI1j









DCnC2c I




I

347




flrhLLWlbblilU












-r




~ C- (f







d ~ tQ



ltlfm1lu r--------------~~------------~--L-~~--------------------------------------~


0930 - 0945 U

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1---11--15---1-1-30-tl--+-lJ

1lJ~~



belLtlvJbbmlW




2 ~ If d IV

iLit) VNiCl


~ 4 rlRIVl D lRliW

ltV lt5 1ilWVliWW




(Boophilus spp)



1 1gt I if tgt





r ~




Abstract










Email ppittakkuacth





o 0

fl1IJ1





~


2t $I I

























t



~










dV ff

vmlUVl (Prevent)


it W l V







explusmn






tt j I






bull I I



12 24


n~3JVl~fltll



n~Jjfllwll-l




~




























Missouri USA



228-23)









29(4) 341-349














Ltd UK




No JITMM201lfSC132

21 October 2011










Yours sincerely





































amp0

~u 41

0 Jt21

bull Tnmiddotl1~(

PCNAmdC~

3D

20

10 ~

rAe PAC CC SC


I













Jenga








3

5

- ~

8

11

13

15

20

Page 25

26

28

30

32

35

Xl

Proceedings


(ASVP) 2011 amp












Organized by

4VIJ


P03middot














-












I

ntrll I ~il

_ ti

-lr r bull ~lt ~

29


OPS2

OPS3

OPS4

OPSS

OPS6

OPS 7

POI

P02

P 03

P04

P 05

P 06






Jenga



3

5

8

11

13

L5

20

Page 25

26

28

30

32

35

Xl

Proceedings


(ASVP) 2011 amp






l Jmiddot~




Organized by

~VI)


ABSTRACTS



































I lt I i lt~





Thailand







confirmed by PCR










213 023 003 008 007 007 and





t Poster No Page



















in the Gr~~~~~~h~~~~~b-r~g~~ 149













(JITMM 2011)


1-2 December 2011


















l1UU~fI1j7jli1 fI1j






flrhLLWlbblilU












-r




~ C- (f







d ~ tQ



ltlfm1lu r--------------~~------------~--L-~~--------------------------------------~


0930 - 0945 U

--- shy -----------------------------------------



JeurollflnLflUlJ



1100 - 1115 tl -r I j

1---11--15---1-1-30-tl--+-lJ

1lJ~~



belLtlvJbbmlW




2 ~ If d IV

iLit) VNiCl


~ 4 rlRIVl D lRliW

ltV lt5 1ilWVliWW




(Boophilus spp)



1 1gt I if tgt





r ~




Abstract










Email ppittakkuacth





o 0

fl1IJ1





~


2t $I I

























t



~










dV ff

vmlUVl (Prevent)


it W l V







explusmn






tt j I






bull I I



12 24


n~3JVl~fltll



n~Jjfllwll-l




~




























Missouri USA



228-23)









29(4) 341-349














Ltd UK




No JITMM201lfSC132

21 October 2011










Yours sincerely





































amp0

~u 41

0 Jt21

bull Tnmiddotl1~(

PCNAmdC~

3D

20

10 ~

rAe PAC CC SC


I













Jenga








3

5

- ~

8

11

13

15

20

Page 25

26

28

30

32

35

Xl

Proceedings


(ASVP) 2011 amp












Organized by

4VIJ


P03middot














-












I

ntrll I ~il

_ ti

-lr r bull ~lt ~

29


OPS2

OPS3

OPS4

OPSS

OPS6

OPS 7

POI

P02

P 03

P04

P 05

P 06






Jenga



3

5

8

11

13

L5

20

Page 25

26

28

30

32

35

Xl

Proceedings


(ASVP) 2011 amp






l Jmiddot~




Organized by

~VI)


ABSTRACTS



































I lt I i lt~





Thailand







confirmed by PCR










213 023 003 008 007 007 and





t Poster No Page



















in the Gr~~~~~~h~~~~~b-r~g~~ 149













(JITMM 2011)


1-2 December 2011


















l1UU~fI1j7jli1 fI1j







d ~ tQ



ltlfm1lu r--------------~~------------~--L-~~--------------------------------------~


0930 - 0945 U

--- shy -----------------------------------------



JeurollflnLflUlJ



1100 - 1115 tl -r I j

1---11--15---1-1-30-tl--+-lJ

1lJ~~



belLtlvJbbmlW




2 ~ If d IV

iLit) VNiCl


~ 4 rlRIVl D lRliW

ltV lt5 1ilWVliWW




(Boophilus spp)



1 1gt I if tgt





r ~




Abstract










Email ppittakkuacth





o 0

fl1IJ1





~


2t $I I

























t



~










dV ff

vmlUVl (Prevent)


it W l V







explusmn






tt j I






bull I I



12 24


n~3JVl~fltll



n~Jjfllwll-l




~




























Missouri USA



228-23)









29(4) 341-349














Ltd UK




No JITMM201lfSC132

21 October 2011










Yours sincerely





































amp0

~u 41

0 Jt21

bull Tnmiddotl1~(

PCNAmdC~

3D

20

10 ~

rAe PAC CC SC


I













Jenga








3

5

- ~

8

11

13

15

20

Page 25

26

28

30

32

35

Xl

Proceedings


(ASVP) 2011 amp












Organized by

4VIJ


P03middot














-












I

ntrll I ~il

_ ti

-lr r bull ~lt ~

29


OPS2

OPS3

OPS4

OPSS

OPS6

OPS 7

POI

P02

P 03

P04

P 05

P 06






Jenga



3

5

8

11

13

L5

20

Page 25

26

28

30

32

35

Xl

Proceedings


(ASVP) 2011 amp






l Jmiddot~




Organized by

~VI)


ABSTRACTS



































I lt I i lt~





Thailand







confirmed by PCR










213 023 003 008 007 007 and





t Poster No Page



















in the Gr~~~~~~h~~~~~b-r~g~~ 149













(JITMM 2011)


1-2 December 2011


















l1UU~fI1j7jli1 fI1j



2 ~ If d IV

iLit) VNiCl


~ 4 rlRIVl D lRliW

ltV lt5 1ilWVliWW




(Boophilus spp)



1 1gt I if tgt





r ~




Abstract










Email ppittakkuacth





o 0

fl1IJ1





~


2t $I I

























t



~










dV ff

vmlUVl (Prevent)


it W l V







explusmn






tt j I






bull I I



12 24


n~3JVl~fltll



n~Jjfllwll-l




~




























Missouri USA



228-23)









29(4) 341-349














Ltd UK




No JITMM201lfSC132

21 October 2011










Yours sincerely





































amp0

~u 41

0 Jt21

bull Tnmiddotl1~(

PCNAmdC~

3D

20

10 ~

rAe PAC CC SC


I













Jenga








3

5

- ~

8

11

13

15

20

Page 25

26

28

30

32

35

Xl

Proceedings


(ASVP) 2011 amp












Organized by

4VIJ


P03middot














-












I

ntrll I ~il

_ ti

-lr r bull ~lt ~

29


OPS2

OPS3

OPS4

OPSS

OPS6

OPS 7

POI

P02

P 03

P04

P 05

P 06






Jenga



3

5

8

11

13

L5

20

Page 25

26

28

30

32

35

Xl

Proceedings


(ASVP) 2011 amp






l Jmiddot~




Organized by

~VI)


ABSTRACTS



































I lt I i lt~





Thailand







confirmed by PCR










213 023 003 008 007 007 and





t Poster No Page



















in the Gr~~~~~~h~~~~~b-r~g~~ 149













(JITMM 2011)


1-2 December 2011


















l1UU~fI1j7jli1 fI1j





(Boophilus spp)



1 1gt I if tgt





r ~




Abstract










Email ppittakkuacth





o 0

fl1IJ1





~


2t $I I

























t



~










dV ff

vmlUVl (Prevent)


it W l V







explusmn






tt j I






bull I I



12 24


n~3JVl~fltll



n~Jjfllwll-l




~




























Missouri USA



228-23)









29(4) 341-349














Ltd UK




No JITMM201lfSC132

21 October 2011










Yours sincerely





































amp0

~u 41

0 Jt21

bull Tnmiddotl1~(

PCNAmdC~

3D

20

10 ~

rAe PAC CC SC


I













Jenga








3

5

- ~

8

11

13

15

20

Page 25

26

28

30

32

35

Xl

Proceedings


(ASVP) 2011 amp












Organized by

4VIJ


P03middot














-












I

ntrll I ~il

_ ti

-lr r bull ~lt ~

29


OPS2

OPS3

OPS4

OPSS

OPS6

OPS 7

POI

P02

P 03

P04

P 05

P 06






Jenga



3

5

8

11

13

L5

20

Page 25

26

28

30

32

35

Xl

Proceedings


(ASVP) 2011 amp






l Jmiddot~




Organized by

~VI)


ABSTRACTS



































I lt I i lt~





Thailand







confirmed by PCR










213 023 003 008 007 007 and





t Poster No Page



















in the Gr~~~~~~h~~~~~b-r~g~~ 149













(JITMM 2011)


1-2 December 2011


















l1UU~fI1j7jli1 fI1j



o 0

fl1IJ1





~


2t $I I

























t



~










dV ff

vmlUVl (Prevent)


it W l V







explusmn






tt j I






bull I I



12 24


n~3JVl~fltll



n~Jjfllwll-l




~




























Missouri USA



228-23)









29(4) 341-349














Ltd UK




No JITMM201lfSC132

21 October 2011










Yours sincerely





































amp0

~u 41

0 Jt21

bull Tnmiddotl1~(

PCNAmdC~

3D

20

10 ~

rAe PAC CC SC


I













Jenga








3

5

- ~

8

11

13

15

20

Page 25

26

28

30

32

35

Xl

Proceedings


(ASVP) 2011 amp












Organized by

4VIJ


P03middot














-












I

ntrll I ~il

_ ti

-lr r bull ~lt ~

29


OPS2

OPS3

OPS4

OPSS

OPS6

OPS 7

POI

P02

P 03

P04

P 05

P 06






Jenga



3

5

8

11

13

L5

20

Page 25

26

28

30

32

35

Xl

Proceedings


(ASVP) 2011 amp






l Jmiddot~




Organized by

~VI)


ABSTRACTS



































I lt I i lt~





Thailand







confirmed by PCR










213 023 003 008 007 007 and





t Poster No Page



















in the Gr~~~~~~h~~~~~b-r~g~~ 149













(JITMM 2011)


1-2 December 2011


















l1UU~fI1j7jli1 fI1j





t



~










dV ff

vmlUVl (Prevent)


it W l V







explusmn






tt j I






bull I I



12 24


n~3JVl~fltll



n~Jjfllwll-l




~




























Missouri USA



228-23)









29(4) 341-349














Ltd UK




No JITMM201lfSC132

21 October 2011










Yours sincerely





































amp0

~u 41

0 Jt21

bull Tnmiddotl1~(

PCNAmdC~

3D

20

10 ~

rAe PAC CC SC


I













Jenga








3

5

- ~

8

11

13

15

20

Page 25

26

28

30

32

35

Xl

Proceedings


(ASVP) 2011 amp












Organized by

4VIJ


P03middot














-












I

ntrll I ~il

_ ti

-lr r bull ~lt ~

29


OPS2

OPS3

OPS4

OPSS

OPS6

OPS 7

POI

P02

P 03

P04

P 05

P 06






Jenga



3

5

8

11

13

L5

20

Page 25

26

28

30

32

35

Xl

Proceedings


(ASVP) 2011 amp






l Jmiddot~




Organized by

~VI)


ABSTRACTS



































I lt I i lt~





Thailand







confirmed by PCR










213 023 003 008 007 007 and





t Poster No Page



















in the Gr~~~~~~h~~~~~b-r~g~~ 149













(JITMM 2011)


1-2 December 2011


















l1UU~fI1j7jli1 fI1j




tt j I






bull I I



12 24


n~3JVl~fltll



n~Jjfllwll-l




~




























Missouri USA



228-23)









29(4) 341-349














Ltd UK




No JITMM201lfSC132

21 October 2011










Yours sincerely





































amp0

~u 41

0 Jt21

bull Tnmiddotl1~(

PCNAmdC~

3D

20

10 ~

rAe PAC CC SC


I













Jenga








3

5

- ~

8

11

13

15

20

Page 25

26

28

30

32

35

Xl

Proceedings


(ASVP) 2011 amp












Organized by

4VIJ


P03middot














-












I

ntrll I ~il

_ ti

-lr r bull ~lt ~

29


OPS2

OPS3

OPS4

OPSS

OPS6

OPS 7

POI

P02

P 03

P04

P 05

P 06






Jenga



3

5

8

11

13

L5

20

Page 25

26

28

30

32

35

Xl

Proceedings


(ASVP) 2011 amp






l Jmiddot~




Organized by

~VI)


ABSTRACTS



































I lt I i lt~





Thailand







confirmed by PCR










213 023 003 008 007 007 and





t Poster No Page



















in the Gr~~~~~~h~~~~~b-r~g~~ 149













(JITMM 2011)


1-2 December 2011


















l1UU~fI1j7jli1 fI1j

























Missouri USA



228-23)









29(4) 341-349














Ltd UK




No JITMM201lfSC132

21 October 2011










Yours sincerely





































amp0

~u 41

0 Jt21

bull Tnmiddotl1~(

PCNAmdC~

3D

20

10 ~

rAe PAC CC SC


I













Jenga








3

5

- ~

8

11

13

15

20

Page 25

26

28

30

32

35

Xl

Proceedings


(ASVP) 2011 amp












Organized by

4VIJ


P03middot














-












I

ntrll I ~il

_ ti

-lr r bull ~lt ~

29


OPS2

OPS3

OPS4

OPSS

OPS6

OPS 7

POI

P02

P 03

P04

P 05

P 06






Jenga



3

5

8

11

13

L5

20

Page 25

26

28

30

32

35

Xl

Proceedings


(ASVP) 2011 amp






l Jmiddot~




Organized by

~VI)


ABSTRACTS



































I lt I i lt~





Thailand







confirmed by PCR










213 023 003 008 007 007 and





t Poster No Page



















in the Gr~~~~~~h~~~~~b-r~g~~ 149













(JITMM 2011)


1-2 December 2011


















l1UU~fI1j7jli1 fI1j









29(4) 341-349














Ltd UK




No JITMM201lfSC132

21 October 2011










Yours sincerely





































amp0

~u 41

0 Jt21

bull Tnmiddotl1~(

PCNAmdC~

3D

20

10 ~

rAe PAC CC SC


I













Jenga








3

5

- ~

8

11

13

15

20

Page 25

26

28

30

32

35

Xl

Proceedings


(ASVP) 2011 amp












Organized by

4VIJ


P03middot














-












I

ntrll I ~il

_ ti

-lr r bull ~lt ~

29


OPS2

OPS3

OPS4

OPSS

OPS6

OPS 7

POI

P02

P 03

P04

P 05

P 06






Jenga



3

5

8

11

13

L5

20

Page 25

26

28

30

32

35

Xl

Proceedings


(ASVP) 2011 amp






l Jmiddot~




Organized by

~VI)


ABSTRACTS



































I lt I i lt~





Thailand







confirmed by PCR










213 023 003 008 007 007 and





t Poster No Page



















in the Gr~~~~~~h~~~~~b-r~g~~ 149













(JITMM 2011)


1-2 December 2011


















l1UU~fI1j7jli1 fI1j



No JITMM201lfSC132

21 October 2011










Yours sincerely





































amp0

~u 41

0 Jt21

bull Tnmiddotl1~(

PCNAmdC~

3D

20

10 ~

rAe PAC CC SC


I













Jenga








3

5

- ~

8

11

13

15

20

Page 25

26

28

30

32

35

Xl

Proceedings


(ASVP) 2011 amp












Organized by

4VIJ


P03middot














-












I

ntrll I ~il

_ ti

-lr r bull ~lt ~

29


OPS2

OPS3

OPS4

OPSS

OPS6

OPS 7

POI

P02

P 03

P04

P 05

P 06






Jenga



3

5

8

11

13

L5

20

Page 25

26

28

30

32

35

Xl

Proceedings


(ASVP) 2011 amp






l Jmiddot~




Organized by

~VI)


ABSTRACTS



































I lt I i lt~





Thailand







confirmed by PCR










213 023 003 008 007 007 and





t Poster No Page



















in the Gr~~~~~~h~~~~~b-r~g~~ 149













(JITMM 2011)


1-2 December 2011


















l1UU~fI1j7jli1 fI1j





































amp0

~u 41

0 Jt21

bull Tnmiddotl1~(

PCNAmdC~

3D

20

10 ~

rAe PAC CC SC


I













Jenga








3

5

- ~

8

11

13

15

20

Page 25

26

28

30

32

35

Xl

Proceedings


(ASVP) 2011 amp












Organized by

4VIJ


P03middot














-












I

ntrll I ~il

_ ti

-lr r bull ~lt ~

29


OPS2

OPS3

OPS4

OPSS

OPS6

OPS 7

POI

P02

P 03

P04

P 05

P 06






Jenga



3

5

8

11

13

L5

20

Page 25

26

28

30

32

35

Xl

Proceedings


(ASVP) 2011 amp






l Jmiddot~




Organized by

~VI)


ABSTRACTS



































I lt I i lt~





Thailand







confirmed by PCR










213 023 003 008 007 007 and





t Poster No Page



















in the Gr~~~~~~h~~~~~b-r~g~~ 149













(JITMM 2011)


1-2 December 2011


















l1UU~fI1j7jli1 fI1j















Jenga








3

5

- ~

8

11

13

15

20

Page 25

26

28

30

32

35

Xl

Proceedings


(ASVP) 2011 amp












Organized by

4VIJ


P03middot














-












I

ntrll I ~il

_ ti

-lr r bull ~lt ~

29


OPS2

OPS3

OPS4

OPSS

OPS6

OPS 7

POI

P02

P 03

P04

P 05

P 06






Jenga



3

5

8

11

13

L5

20

Page 25

26

28

30

32

35

Xl

Proceedings


(ASVP) 2011 amp






l Jmiddot~




Organized by

~VI)


ABSTRACTS



































I lt I i lt~





Thailand







confirmed by PCR










213 023 003 008 007 007 and





t Poster No Page



















in the Gr~~~~~~h~~~~~b-r~g~~ 149













(JITMM 2011)


1-2 December 2011


















l1UU~fI1j7jli1 fI1j



Proceedings


(ASVP) 2011 amp












Organized by

4VIJ


P03middot














-












I

ntrll I ~il

_ ti

-lr r bull ~lt ~

29


OPS2

OPS3

OPS4

OPSS

OPS6

OPS 7

POI

P02

P 03

P04

P 05

P 06






Jenga



3

5

8

11

13

L5

20

Page 25

26

28

30

32

35

Xl

Proceedings


(ASVP) 2011 amp






l Jmiddot~




Organized by

~VI)


ABSTRACTS



































I lt I i lt~





Thailand







confirmed by PCR










213 023 003 008 007 007 and





t Poster No Page



















in the Gr~~~~~~h~~~~~b-r~g~~ 149













(JITMM 2011)


1-2 December 2011


















l1UU~fI1j7jli1 fI1j










Organized by

4VIJ


P03middot














-












I

ntrll I ~il

_ ti

-lr r bull ~lt ~

29


OPS2

OPS3

OPS4

OPSS

OPS6

OPS 7

POI

P02

P 03

P04

P 05

P 06






Jenga



3

5

8

11

13

L5

20

Page 25

26

28

30

32

35

Xl

Proceedings


(ASVP) 2011 amp






l Jmiddot~




Organized by

~VI)


ABSTRACTS



































I lt I i lt~





Thailand







confirmed by PCR










213 023 003 008 007 007 and





t Poster No Page



















in the Gr~~~~~~h~~~~~b-r~g~~ 149













(JITMM 2011)


1-2 December 2011


















l1UU~fI1j7jli1 fI1j



P03middot














-












I

ntrll I ~il

_ ti

-lr r bull ~lt ~

29


OPS2

OPS3

OPS4

OPSS

OPS6

OPS 7

POI

P02

P 03

P04

P 05

P 06






Jenga



3

5

8

11

13

L5

20

Page 25

26

28

30

32

35

Xl

Proceedings


(ASVP) 2011 amp






l Jmiddot~




Organized by

~VI)


ABSTRACTS



































I lt I i lt~





Thailand







confirmed by PCR










213 023 003 008 007 007 and





t Poster No Page



















in the Gr~~~~~~h~~~~~b-r~g~~ 149













(JITMM 2011)


1-2 December 2011


















l1UU~fI1j7jli1 fI1j












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29


OPS2

OPS3

OPS4

OPSS

OPS6

OPS 7

POI

P02

P 03

P04

P 05

P 06






Jenga



3

5

8

11

13

L5

20

Page 25

26

28

30

32

35

Xl

Proceedings


(ASVP) 2011 amp






l Jmiddot~




Organized by

~VI)


ABSTRACTS



































I lt I i lt~





Thailand







confirmed by PCR










213 023 003 008 007 007 and





t Poster No Page



















in the Gr~~~~~~h~~~~~b-r~g~~ 149













(JITMM 2011)


1-2 December 2011


















l1UU~fI1j7jli1 fI1j




OPS2

OPS3

OPS4

OPSS

OPS6

OPS 7

POI

P02

P 03

P04

P 05

P 06






Jenga



3

5

8

11

13

L5

20

Page 25

26

28

30

32

35

Xl

Proceedings


(ASVP) 2011 amp






l Jmiddot~




Organized by

~VI)


ABSTRACTS



































I lt I i lt~





Thailand







confirmed by PCR










213 023 003 008 007 007 and





t Poster No Page



















in the Gr~~~~~~h~~~~~b-r~g~~ 149













(JITMM 2011)


1-2 December 2011


















l1UU~fI1j7jli1 fI1j



Proceedings


(ASVP) 2011 amp






l Jmiddot~




Organized by

~VI)


ABSTRACTS



































I lt I i lt~





Thailand







confirmed by PCR










213 023 003 008 007 007 and





t Poster No Page



















in the Gr~~~~~~h~~~~~b-r~g~~ 149













(JITMM 2011)


1-2 December 2011


















l1UU~fI1j7jli1 fI1j




l Jmiddot~




Organized by

~VI)


ABSTRACTS



































I lt I i lt~





Thailand







confirmed by PCR










213 023 003 008 007 007 and





t Poster No Page



















in the Gr~~~~~~h~~~~~b-r~g~~ 149













(JITMM 2011)


1-2 December 2011


















l1UU~fI1j7jli1 fI1j



ABSTRACTS



































I lt I i lt~





Thailand







confirmed by PCR










213 023 003 008 007 007 and





t Poster No Page



















in the Gr~~~~~~h~~~~~b-r~g~~ 149













(JITMM 2011)


1-2 December 2011


















l1UU~fI1j7jli1 fI1j




t Poster No Page



















in the Gr~~~~~~h~~~~~b-r~g~~ 149













(JITMM 2011)


1-2 December 2011


















l1UU~fI1j7jli1 fI1j




(JITMM 2011)


1-2 December 2011


















l1UU~fI1j7jli1 fI1j















l1UU~fI1j7jli1 fI1j



1. :amb {'jut 'wvltj1 · 2015-06-30 · poster presentation, is submitted and is under ....

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