1 all of the following statements would be supported by the cell theory except a.all living things...
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All of the following statements would be supported by the cell theory EXCEPT
a. All living things are made of cells.b. All cells come from other cells.c. The cell is the basic functional unit of an organism.d. Cells reproduce to form additional cells.e. Every animal cell is exactly the same in structure and function.
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The smallest, simplest collection of matter that exhibits traits of life isa. an atom b. a nucleusc. a chloroplastd. a celle. a tissue
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Chapter 4Cell Structure and
FunctionNucleolusNucleus
Nuclear envelope
Ribosome (attached)Ribosome (free)
Cell Membrane
Rough endoplasmic reticulum
Golgi apparatus
Mitochondrion
Smooth endoplasmicreticulum
Centrioles
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A CELL is . . . made of MOLECULES
_______ ___________ ___________ATOMS MOLECULES ORGANELLES
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WHICH IS BIGGER?
_________ > _____________ > ___________Plant cell Animal cell bacteria
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Note
• The shapes shown on the last slide are merely a generic version of a plant, animal, and bacteria cell.– In other words, not all plants are shaped like a
box and animal cells circular• That is simply done to show that plants have cell
walls and animal cells do not
– Every type of cell is shaped a particular way to fit whatever its function may be
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ALL LIVING THINGS ARE MADE OF CELLS
• PROKARYOTES• Lack nucleus• Lack membrane
bound organelles
• EUKARYOTES• Have nucleus• Have membrane
bound organelles
Bacterial Cell
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All cells (both prokaryotic and eukaryotic) have several basic features in
common1. Plasma (cell) membrane
2. Chromosomes which carry genes made of DNA (although the shape of eukaryotic chromosomes and a prokaryotic chromosome are different)
3. Cytoplasm
4. Cytoskeleton
5. Ribosomes that make proteins (although the shape of eukaryotic ribosomes and prokaryotic ribosomes are different)
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1. CELL MEMBRANE(also called plasma membrane)
Made mainly of phospholipids & proteins
Controls what enters and leaves the cell
Outsideof cell
Insideof cell(cytoplasm)
Cellmembrane
Proteins
Proteinchannel Lipid bilayer
Carbohydratechains
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Cell membrane continued -PHOSPHOLIPID
• Phospholipid has 2 main regions:
• Head negative charge, hydrophilic
• 2 fatty acid Tails nonpolar, hydrophobic
HYDROPHILIC
HYDROPHOBIC 10
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Cell membrane continued - PHOSPHOLIPID BILAYER
• They form a two-layered sheet called the phospholipid bilayer.
• Hydrophilic heads face out• Hydrophobic tails tucked inward.
• Proteins are embedded on the membrane,
or attached to the surface.
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Cell membrane continued • Proteins are embedded on the membrane,
or attached to the surface.
Proteins that stick on the surface = PERIPHERAL
(either inside or outside of cell)
Proteins that stick INTO membrane = Integral(can go part way in or all the way through) 12
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Cell membrane continued - Permeability
• Nonpolar molecules such as O2 and CO2 can easily pass through the hydrophobic interior.
• Proteins in the membrane form channels that allow specific molecules to cross.
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Recognize “self”
Cell membrane continued - GLYCOPROTEINS
GLYCOPROTEINS are PROTEINS with carbohydrates attached.
Play a role in cell-cell interactions. 14
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Cell membrane continued - TRANSPORT PROTEINShelp move substances across the cell membrane
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Cell membranes MOVE!
Molecules in cell membranes are
constantly moving and changing16
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Cell membrane continued - WHAT DOES IT DO?
Acts as a boundaryControls what enters and leaves cell 17
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2. Chromosomes - Genetic material (DNA)
• Chromatin – thin fibers of DNA and proteins that look like a diffuse mass.
• As the cell prepares to divide, the DNA is copied and the chromatin coils up (becoming visible with a light microscope) into the structure we know as chromosomes.
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3. CYTOPLASM (Between nucleus and cell membrane)
ORGANELLE-small structure with a specific function (job)
Organelles suspended in gel-like goo
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4. CYTOSKELETON
• Helps cell maintain shape• Help move organelles around
Network of protein fibers, found throughout the cytoplasm.
Three main types of fibers make up the cytoskeleton:1. Microfilaments (the thinnest)2. Microtubules (the thickest)3. Intermediate filaments (in between)
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Cytoskeleton continued -Microfilaments
• Solid rods made of the protein actin, in a twisted double chain
• Supports the cell’s shape & involved in cell movement.– Ex: Actin and another protein
myosin interact to cause contraction of muscle cells.
Microfilament
Actin subunit
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Cytoskeleton continued - Microtubules
• Straight hollow tubes made of the protein tubulin.
• Easily disassembled & subunits reused elsewhere.
• Give shape and support to the cell & acts as tracks along which organelles with motor proteins can move.– Ex: lysosomes move along track to
reach a food vacuole.– Ex: guide chromosomes during cell
division
• The main component of cilia & flagella
Tubulin subunit
Microtubule
Nucleus
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Cytoskeleton continued - Intermediate filaments• Made of various proteins,
and has a ropelike structure.
• Reinforce cell shape & anchors organelles. – Ex: Nucleus is held in place
by a cage of intermediate filaments.
– Ex: Our outer layer of skin consists of dead cells containing intermediate filaments made of keratin proteins.
Nucleus
Intermediate filament23
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Characteristics of prokaryotic cells that are not found in eukaryotic cells
1. Bacterial chromosome – present as a single loop of DNA (eukaryotic chromosomes come in many pairs which we will discuss in detail later)
2. Nucleoid – region where the prokaryotic cells DNA is located (not enclosed by a membrane like eukaryotic cells)
3. Pili – attachment structures on the surface of some prokaryotes
4. Cell wall – plants (which are eukaryotic cells) also have cell walls but they are made of a different structure
5. Capsule – jellylike outer coating outside of cell wall
Note: Flagella and cilia are also found in animal cells (not plants), but they are included here because the diagram shows them. Sperm have flagella, cells in your wind pipe have cilia, and so forth.
• Flagella (long tail like structure) and cilia (many hair like structures) used for locomotion (some types of eukaryotic cells do have flagella or cilia) 24
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Typical Prokaryotic cell
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Fig. 4-3b
Nucleoid
Ribosomes
Plasma membrane
Cell wall
Capsule
Flagella
Bacterialchromosome
Pili
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Nucleoid• The DNA of a prokaryotic cell is coiled into a region
called the nucleoid. • Unlike the eukaryotic nucleus, it has no membrane that
surrounds the DNA.• The DNA of a prokaryote is in a single circular loop. The
DNA of eukaryotes (like you) are in chromosome form (we’ll discuss this next unit).
• Also, the ribosomes of prokaryotic cells are smaller.• Antibiotics are designed to target (smaller) ribosomes of
prokaryotic cells (bacteria), interrupting protein synthesis. – Antibiotics do not harm eukaryotic cells.
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Cilia & Flagella
• Both consist of microtubules wrapped in an extension of the cell membrane.
• Ring of 9 microtubule “doublets” surrounds a central pair of microtubules. – (called 9 + 2 pattern)
Outer microtubuledoublet
Centralmicrotubules
Plasmamembrane
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• Cilia – short numerous appendages that propel cell forward.– Found on cells lining the human windpipe,
which sweeps mucus & trapped debris out of our lungs.
Cilia
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• Flagella – longer, and limited to one or a few per cell.– Sperm have flagella for movement.
Flagellum
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Which of the following hierarchies is correct?
a. molecule → organelle → cell → tissue → organ → organism b. organelle → cell → nucleus → tissue → organ → organ system c. nucleus →atom → cell → organelle → tissue → organ d. mitochondria → organelle → nucleus → cell → organism → tissuee. tissue → nucleus → cell → organelle → organ → molecule
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Which of the following structures would be used to identify a prokaryotic cell?
a. cell membrane, nucleus, and DNAb. cell wall, flagella, and chloroplastsc. cytoplasm, smooth and rough endoplasmic reticulumd. mitochondria, Golgi body, and lysosomese. cell membrane, ribosomes, and a chromosome
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Biochemists have created an artificial cell membrane that they hope to insert into artificial red blood cells for future dehydration and transportation to outer space. When scientists stain natural cell membranes with a heavy metal, they can view the membranes with an electron microscope. The heavy metal stains the polar hydrophilic heads of phospholipid membranes. If the artificial membranes resemble natural membranes, what area(s) when viewed under a microscope would appear stained? (Note: Shading represents stains) a.
b.
c.
d.
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How did the first eukaryotic cells form?
• ENDOSYMBIOSIS
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Endosymbiosis• Mitochondria & chloroplasts were formerly small
prokaryotes that began living within larger cells.• May have occurred as undigested prey or internal
parasites.• Forming symbiotic relationship.
– Host cell uses nutrients released from photosynthetic endosymbionts.
– Endosymbionts are provided protection by host cell.
• Over time, they would become more interdependent, eventually becoming a single organism.
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Evidence of Endosymbiosis
• Mitochondria & chloroplasts both contain their own DNA & ribosomes.
• Their ribosomes are more similar to prokaryotic ribosomes.
• Both reproduce by a splitting process similar to that of prokaryotes.
• Both are surrounded by two membranes.
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Fig. 4-16
Engulfing ofphotosyntheticprokaryote
Chloroplast
Mitochondrion
Somecells
Host cell
Mitochondrion
Host cellEngulfing of aerobicprokaryote
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Endosymbiosis explains the evolution of eukaryotic cells. Which of the following descriptions best fits this theory?a. Eukaryotic cells were the first cells. Prokaryotic cells came later as they are more complex. b. Organelles, such as mitochondria and chloroplasts, originated as prokaryotes exhibited a mutually beneficial relationship after a larger prokaryotes tried to engulf a smaller prokaryote. c. All cells must have mitochondria and chloroplasts to survive. Endosymbiosis explains why this is true.d. Prokaryotes developed membrane-bound organelles and still have them today.
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Which of the following are actual evidence supporting the endosymbiotic theory (endosymbiosis) for the origin of eukaryotes?
a. Mitochondria & chloroplasts both contain their own DNA & ribosomes.b. Both mitochondria & chloroplast reproduce by a splitting process similar to that of prokaryotes.c. Both mitochondria & chloroplast are surrounded by two membranes.d. All of the abovee. None of the above
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Organelles that are unique amongst eukaryotes (basically plants and animals) but not present in prokaryotes (bacteria)
1. Nucleus
2. Nucleolus
3. Endoplasmic reticulum
4. Golgi Apparatus (also called golgi body)
5. Mitochondria
6. Peroxisome
7. Vacuoles40
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1. NUCLEUS• Contains cell’s genetic material (DNA)
• Controls cell’s activity by directing protein synthesis.
• Largest organelle
in animal cells
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1. NUCLEUS• Surrounded by NUCLEAR ENVELOPE • It is a double membrane perforated with
protein lined pores.
(also called NUCLEAR MEMBRANE)
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1. NUCLEUS
NUCLEAR PORES • Openings that control the flow of materials into and
out of the nucleus
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2. NUCLEOLUS• Dark spot in nucleus• Site where ribosomal RNA (rRNA) is synthesized
according to the instructions of DNA. • Proteins brought in through the nuclear pores are
assembled with the rRNA to build a subunit of ribosomes.
• These subunits exit through the nuclear pores where they will be joined to form functional ribosomes. Large
subunitSmallsubunit
Diagram of a ribosome 44
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Another type of RNA• The nucleus directs protein synthesis with
messenger RNA (mRNA).
• mRNA is a short transcription (copy) of DNA that exits through the nuclear pores where it is translated by ribosomes into amino acid sequences of proteins.
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RIBOSOMES for eukaryotes can be found in several places
Can be attached to:
• Nucleus
• Rough ER OR
• Free in cytoplasm
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3. ENDOPLASMIC RETICULUM
Two kinds:SMOOTH or ROUGH
Network of flattened sacs and tubules
“endoplasmic” – within the cell
“reticulum” – little net
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Smooth ER
• NO ribosomes attached
• Has enzymes for special tasks:
• Example: Cells of ovaries & testes synthesize the steroid sex hormones. – Enzymes synthesize lipids
• (oils, phospholipids, & steroids)48
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Another example (pg.60)• Liver cells help process drugs and other harmful
substances.• Cells exposed to drugs cause the amount of smooth
ER (w/ detoxifying enzymes) to increase• This increases the rate of detoxification. • Causing increasing tolerance to the drug.• Now, dose must be increased to be effective.
• Another complication is that enzymes often cannot distinguish among related drugs.
• So an increase in tolerance in one drug, may cause one in another drug.– Ex: barbiturate use can decrease effectiveness of
antibiotics. 49
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Another example (pg.60)
• In muscle cells, smooth ER membrane pumps calcium ions into the interior of the ER.
• When a nerve signal stimulates a muscle, calcium ions rush from the smooth ER into the cytoplasm….triggering a contraction of the cell.
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Rough ER
• Rough ER membrane enlarges.
• Phospholipids made by enzymes of the ER are inserted into the membrane.
• Some of this membrane is passed onto other membranes in vesicles.
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Rough ER
• Ribosomes attached. (rough) • These ribosomes make
proteins, which are inserted into the ER membrane, where they are modified and transported to other organelles.
• Example: Insulin. Secreted by cells in the pancreas.
Ribosome
1
2
3
4
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After leaving the ER, many transport vesicles travel to the Golgi apparatus…
Transportvesiclefrom ER
Golgi apparatusRough ER
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4. GOLGI APPARATUS (BODY)• Discovered by Camillo Golgi with a light
microscope.• Confirmed later with an electron microscope.
Electron micrograph
Flattened sacs stacked on top of each other.Sacs are NOT interconnected like ER.
Number of Golgi stacks correlates with how active the cell is in secreting proteins.
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GOLGI APPARATUS (BODY)• Molecular warehouse & finishing factory.• Receives & modifies products from ER.• One side is receiving dock for transport vesicles• Other side is shipping dock giving off vesicles.• “maturation model” entire sacs mature as they
move from the receiving end to the shipping end, carrying and modifying their cargo as they go.
• Exiting vesicles move
to the cell membrane for
export form the cell.
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5. MitochondrionMitochondria (plural)
• cellular respiration - converts food energy into molecular energy.
• ATP (adenosine triphosphate). – The main energy source for cellular work.
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Enclosed by 2 membranes.
The inner membrane is highly folded, and contains proteins that make ATP.
The folds (cristae) increase membrane surface area, enhancing ability to produce ATP.
Has 2 internal compartments
1. Internal membrane space – narrow region between inner and outer membrane.
2. Mitochondrial matrix – contains the mitochondrial DNA, ribosomes, & enzymes.
Mitochondrion’s structure
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Found in plant & animal cells! 60
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MITOCHONDRIA
• You inherit your mitochondria from your mother!
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WHAT DOES IT DO?
Converts glucose to ATP
“Powerplant of cell”
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6. Peroxisome
• Vesicle that neutralizes dangerous oxygen compounds– contains catalase that breaks down H2O2
– Detoxification of alcohol
• Also breaks down fatty acids to be used as fuel.
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Zellweger Syndrome • Defective genes reduce or eliminate the presence
of peroxisomes • Results in build up of iron and copper in blood
and tissue • Symptoms include an enlarged liver; facial
deformities, and neurological abnormalities such as mental retardation and seizures.
• Most infants do not survive past the first 6 months, and usually succumb to respiratory
distress, gastrointestinal bleeding, or liver failure.
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7. VACUOLES
Membrane sacs used for storage
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VACUOLES• Storage space for WATER,
salts, proteins (enzymes), carbohydrates, and waste
• Maintains internal pH
•Largest structure in plant cells•Small in animal cells•No vacuoles in bacteria cells
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Contractile vacuoles
• Paramecium (unicellular organism, protists)
• Collect excess water from cell, and expels it to the outside of cell.
• Without vacuole, cell fluid would become too diluted to support life, & cell would eventually swell & burst.
Nucleus
Contractilevacuoles
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Food Vacuole
• capture and digestion of food particles
• Fuse with lysosomes to digest food And release nutrients.
• phagocytosis - the ingestion of particulate matter
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Organelles found only in plant cells
1. Cell wall (prokaryotes also have cell walls but they’re made of different material. Animals do not have cell walls)
2. Chloroplast
3. Central Vacuole
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1. CELL WALLSupports and
protects cell
Outside of cell membrane
Made of carbohydrates & proteins
Plant cell walls are mainly cellulose 70
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2. Chloroplasts• Photosynthesizing organelles -
Use energy from sunlight to make own food (glucose)
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Chloroplasts• Has an inner and outer membrane.• Inside inner membrane is a thick fluid called stroma.• Stroma contains the chloroplast DNA & ribosomes.
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Chloroplasts• Thylakoids (a network of connected sacs)
are stacked inside the chloroplast.
• The stacks of thylakoids are called grana
• Grana are the solar power packs – site where green chlorophyll molecules trap solar energy.
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3. Central vacuole• Found in plant cells.
• Can take up more than half the cells volume
• Holds large amounts of water, food & waste
• Plays an important role in plant structure.
• Vacuoles in flower petals contain pigments to attract insects!
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Organelles found only in animal cells (not in plants or
prokaryotes)1. Centrioles
2. Lysosomes – basically the “recycling center” of the cell
– The most common hypothesis as to why plant cells do not need lysosomes is that they have a cell wall that would prevent the majority of material broken down by lysosomes in animal cells from entering plant cells
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1. Centrioles
Appear during cell division to guide chromosomes apart
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Centrioles
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2. LYSOSOMES• Membrane bound sacs that contain digestive
enzymes.• Made by rough ER & transferred to Golgi body.
Serves as a recycling center. Damaged organelles are dismantled, releasing organic molecules for reuse.
* Found in animal cells only! 78
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2. LYSOSOMES• Protists engulf food particles into
vacuoles…
• White blood cells ingest bacteria into vacuoles…
• Lysosomes fuse with these vacuoles and empties its digestive enzymes into them.
Plasmamembrane
LysosomeDigestiveenzymes
Food vacuole
Digestion
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Lysosomal storage disease
• Lack one or more lysosomal enzymes.
• Lysosomes become engorged with undigested material.
• Example: Tay-Sachs disease
• Lipid digesting enzyme is missing
• Brain cells become impaired by accumulation of lipids.
• A child with Tay-Sachs disease will die within a few years.
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Apoptosis• Programmed cell death• Lysosomes help digest
unwanted cells • Cells in developing hands and
feet creates the spaces between fingers & toes.
Apoptosis
Dead cell engulfedand digested byadjacent cell
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Apoptosis plays a role in:
Embryonic developmentNormal body cell maintenance Immune system responsesCancerAIDS infectionTransplant rejection
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Cell to cell communication
• The vast majority of eukaryotic organisms are multicellular
• In order to function properly, these cells must be able to communicate with one another and to transmit information back and forth
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Extracellular matrix (ECM) – Present only in animals
• Helps hold cells together in tissues.
• Protects and supports the plasma membrane.
• Main component: glycoproteins (ex: collagen)
• Integers - membrane proteins that interconnects the ECM to the cytoskeleton.
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Fig. 4-20
EXTRACELLULAR FLUID
Microfilaments
Collagen fiber
Connectingglycoprotein
Integrin
Plasmamembrane
Glycoproteincomplex with longpolysaccharide
CYTOPLASM
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Three types of cell junctions in animal cells
• Tight junction – membranes of neighboring cells are very tightly pressed against each other.
• Prevent leakage of extracellular fluid
Tight junctions
–Example: tissue lines the digestive tract, preventing the contents from leaking into surrounding tissues.
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Three types of cell junctions in animal cells
• Anchoring junction – functions like rivets, fastening cells together into strong sheets.
• Common in tissue subject to stretching or mechanical stress. – Skin & heart muscle
Anchoring junction
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Three types of cell junctions in animal cells
• Gap junctions – channels that allow small molecules to flow through protein lined pores between neighboring cells. – Ex: flow of ions through gap junctions in the
cells of heart muscle coordinates their contraction.
Gap junctions
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Fig. 4-21
Tight junctions
Anchoring junction
Gap junctions
Plasma membranesof adjacent cells
Extracellular matrix89
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Plasmodesmata – Present only in plant cells
• Channels between adjacent plant cells, form a circulatory and communication system connecting the cells in plant tissue.
• Cell membrane & cytoplasm extend through the plasmodesmata, so water and molecules can pass cell to cell.
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Fig. 4-22
Vacuole
Wallsof twoadjacentplant cells
Cytoplasm
Primary cell wall
Plasma membrane
Plasmodesmata
Secondary cell wall
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DIFFERENCES IN ANIMAL CELLS, PLANT CELLS, AND BACTERIA
ANIMAL CELL PLANT CELL BACTERIA
Eukaryotes Eukaryotes Prokaryotes
Cell membrane Cell membrane Cell membrane
Nuclear membrane
Nuclear membrane
NO nuclear membrane
NO cell wall Cell wall made of
CELLULOSE
Cell wall made ofPEPTIDOGLYCAN
Has ribosomes Has ribosomes Has ribosomes
DNA in multiple
chromosomes
DNA in multiple
chromosomes
DNA is a single circular ring
CYTOSKELETON
CYTOSKELETON CYTOSKELETON
Small vacuoles Really big vacuole
NO vacuoles
Has lysosomes NO lysosomes NO lysosomes
Has centrioles NO centrioles NO centrioles
Has mitochondria
Has mitochondria
No mitochondria
NO chloroplasts
Chloroplasts NO chloroplasts
SMALLER SMALL SMALLEST
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USE WORDS FROM THE WORD BANKS TO COMPLETE THE VENN DIAGRAM COMPARISON
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Fig. 4-4b
Smooth endoplasmicreticulum
Rough endoplasmicreticulum
CYTOSKELETON:
NUCLEUS:
Nuclear envelope
Chromosome
NucleolusRibosomes
Golgiapparatus
Plasma membrane
Mitochondrion
Peroxisome
Cell wall
Central vacuoleMicrotubule
Intermediatefilament
Microfilament
Cell wall ofadjacent cell
Chloroplast
Plasmodesmata
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Fig. 4-4a
Smooth endoplasmicreticulum
Roughendoplasmicreticulum
CYTOSKELETON:
NUCLEUS:
Nuclear envelope
Chromosomes
Nucleolus
Ribosomes
Golgiapparatus
Plasma membrane
Mitochondrion
Peroxisome
Centriole
Lysosome
Microtubule
Intermediatefilament
Microfilament 95
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Fig. 4-UN3 a.
b.
c.
d.
e.
f.
g.
h.
i.
j.
k.
l.
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Viruses are not considered living because they are not made of
cells.• They do not have their own plasma membrane, ribosomes,
cytoskeleton, etc. that all cells have (prokaryotic and eukaryotic) which are required to perform MRS. GOCH independently.
• All viruses are made of a protein shell that contains hereditary material (DNA or RNA).
• They survive/reproduce by inserting their DNA into your cells and taking over your cell (using your cell’s ribosomes, etc.) to make more of the virus. Some types of viruses actually insert their DNA into your own so every time you make new cells you are replicating their DNA as well.
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Treating viral diseases
• This is why there is no cure for viral diseases. The viral DNA becomes a part of your DNA so to kill the virus you would have to kill your own cells. This is unlike bacterial diseases where you can simply target the bacteria itself and kill it.
• This means that once you have a viral disease, you will always have it. When you no longer show symptoms, it is because the virus is dormant (inactive) in your body. It could become active again later. There are medications to suppress the virus but none to cure it.
• Examples of viral diseases: – Herpes, Genital Warts, Chickpox, Ebola
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Ebola • Initial infection is caused by close contact with infected animals (bats, chimpanzees). It is then
transmitted through bodily fluids (blood, semen, saliva)• Symptoms
– Nausea and vomiting.
– Diarrhea (may be bloody)
– Red eyes.
– Raised rash.
– Chest pain and cough.
– Stomach pain.
– Severe weight loss.
– Bleeding, usually from the eyes, and bruising (people near death may bleed from other orifices, such as ears, nose and rectum)
• Gaining national exposure because deadliness of disease (50% death rate after infected with virus)• Up until this point, everyone who has contracted the virus has been a healthcare professional treating
someone who has it. These instances have been due to negligence in regards to safety precautions.• Summary: Ebola is generally not contagious until symptoms begin. The threat of a nation wide
epidemic is actually very low due to the nature of how the disease is spread.
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