1% Agarose Gel DNA ElectrophoresisRunning time : 52 minutes

Distance measured from the well to the top of the second band: 4,5 cm

Running voltage: 100 Volt

Solidification time: 14 minutes

Composition: 250 mikroL buffer TAE + 2,5 gram Agarose + 20 mikroL Ethidium Bromide

1 Kb 100 Kb

Second Band

Scale in Cm

2% Agarose Gel DNA ElectrophoresisRunning time : 52 minutesDistance measured from the well to the top of the second band: 3.4 cm

Total Running time: 62 minutesTotal distance measured: 4.5 cm

Running voltage: 100 Volt

Solidification time: 21 minutes

Composition: 500 mL buffer TAE + 10 gram Agarose + 40 mikroL Ethidium Bromide

1 Kb 100 Kb

Second Band

Scale in Cm

3% Agarose Gel DNA ElectrophoresisRunning time : 52 minutesDistance measured from the well to the top of the second band: 2.9 cm

Total Running time: 68 minutesTotal distance measured: 4.5 cm

Running voltage: 100 Volt

Solidification time: 17 minutes

Composition: 250 mikroL buffer TAE + 7.5 gram Agarose + 40 mikroL Ethidium Bromide

1 Kb 100 Kb

Second Band

Scale in Cm

Overall comparisonCross sections of 3 different concentration ofDNA Agarose Gelare illuminated under UV light

1% 2% 3%

Overall Comparison1% 2% 3%

1 kb 1 kb 1 kb100 bp100 bp 100 bp

Increasing the concentration of Agarose gel decreases the electrpphoretic mobility of DNA

Larger Fragments are better resolved in the low agarose concentration while higher concentration allow resolution of smaller DNAs

Comparison5 mikroL of 1 Kb DNA Ladder in 1%,2%

and 3% Agarose Gel Electrophoresis1% 2% 3%

Wells level

3,0 kb

3,0 kb

2,0 kb

2,0 kb

Comparison5 mikroL of 100 bp DNA Ladder in 1%,2% and 3%

Agarose Gel Electrophoresis1% 2% 3%

Wells level

1000 bp

1000 bp

500 bp

500 bp