1 a novel approach to foot dipping robert l. owen and john lawlor
TRANSCRIPT
1
A NOVEL APPROACH TO FOOT DIPPING
Robert L. Owen and John Lawlor
The Problem
• Biosecurity is a year round obligation
• In some parts of the country, foot dipping is impossible at certain times of the year.
• Universally, foot pans are inadequately maintained.
The Solution
1) Ignore that the problem exists
2) Discontinue use of foot pans
3) Utilize pump up sprayers filled with disinfectant
4) How about a dry foot bath?
Question 1
Are foot pan solutions effective at reducing the numbers of bacteria on shoes of hatchery workers?
Total Bacterial Counts from Shoe Swabs
Fresh SolutionAfter 3 hours of use
Active Ingredient
% Change in bacterial count
% Change in bacterial count
Phenol -45.8 +130.5
Quaternary Ammonium
-57.5 +73.3
Water +87.2 +44.8
Question 2
How long does a foot pan solution stay clean in a hatchery environment?
Total bacterial count of the solution
Fresh solution –
Before and after ~25 exposures
3 hours old –
Before and after ~25 exposures
Active ingredient
Change in Bacterial Count
Change in Bacterial Count
Phenol 36 TNTC TNTC TNTC
Quaternary Ammonium 1 12 185 TNTC
Water 19 TNTC TNTC TNTC
Materials and Methods
• A total of 5 dry products were tested
• Early testing was for efficacy of Salmonella control only
• Based on these results 2 of the 5 original products were dropped due to lack of efficacy or cost
• Remaining products were tested versus currently used liquid products
Testing Procedure
• Swab was collected from the sole of the technician’s shoe before stepping into test pan
• Stood in test pan for 10 second contact time
• Shoe was again swabbed after stepping out of test pan
• Samples were directly plated on TSA and incubated 37oC for 18 to 24 hours
• Colonies enumerated by direct count
• >400 classified as TNTC
• Repeated daily until efficacy was lost
ResultsActive Ingredient
% Change in Bacterial Count
Average Residual Life
Powdered detergent + dry bleach
-89.43 3 weeks
Dry Bleach -92.5 3 weeks
Powdered detergent + dry bleach
-86.41 3 weeks
Phenol -74.59 2 weeks
Quaternary ammonium
-95.6 2 weeks
Materials and Methods: Trial 2
• Actually done in the hatchery
• Employee’s shoe was swabbed before stepping into test pan
• Between Chick Room and Break Room
• Real World 5 to 10 second contact time
Results – Trial 2Active Ingredient
% Change in Bacterial count
Average Residual Life
Detergent + Dry Bleach
-92.6 14 days
Dry Bleach -98.06 14 days
Phenol + 10.8 <2 hours
Quaternary ammonium
-23.6 <2 hours
Conclusions
• Duration of effectiveness for solution in foot pans is very short under field conditions
• Dry disinfectants are effective at reducing bacterial numbers on shoes
• Duration of effectiveness for dry disinfectants is substantially longer than for solutions
Practical Applications
• Real goal is to reduce tracking of bacteria – especially Salmonella
• Only part of an overall biosecurity program
Entrance to Chicken House
Biosecurity Building
Inside The Hatchery
Acknowledgments
• Tony Britt, Hatchery Manager, Pikeville, TN
• Technical Support
• Melissa Poulson
• Amanda Harvey
• Tara Stroope
• Lena Shipley
