1

2

Chromosomes and DNAChromosomes and DNA

• Chromosomes are made up of a chemical called DNA.

• They contain genes.

Genes

• Instructions to build proteins.– Examples: gene for hitchhiker’s

thumb, widow’s peak, tongue-rolling…

• Humans have over 30,000 genes encoded in their genome.

3

4

Why do we study DNADNA?

We study DNA for many reasons, e.g.,

• its central importance to all life on Earth,

• medical benefits such as cures for diseases,

• better food crops.

5

DNADNA• DNA DNA is often

called the blueprint of life..

• In simple terms, DNA contains the instructions for making proteins within the cell.

6

DNA by the NumbersDNA by the Numbers• Each cell has about 2

m of DNA.• The average human

has 75 trillion cells.• The average human

has enough DNA to go from the earth to the sun more than 400 times.

• DNA has a diameter of only 0.000000002 m.

The earth is 150 billion mor 93 million miles from the sun.

Packaging

7

Packaging

8

9

The Shape of the The Shape of the MoleculeMolecule

•DNA is a very long polymer.

•The basic shape is like a twisted ladder or zipper.

•This is called a double helix.

10

The Double Helix The Double Helix MoleculeMolecule

•The DNA double helix has two strands twisted together.

http://www.youtube.com/watch?v=4LlBAVhhd-8

11

One Strand of DNAOne Strand of DNA

• One strand of DNA is a polymer of nucleotide.

nucleotide

12

Four DNA nucleotidesFour DNA nucleotides

• Cytosine C• Thymine T

• Adenine A

• Guanine G

13

14

Two Stranded DNATwo Stranded DNA• Remember,

DNA has two strands that fit together something like a zipper.

• The teeth are the nitrogenous bases but why do they stick together?

15

C

C

C

C

N

N

OO

N

C

C

C

C

N NOO

N

N

N C

Hydrogen BondsHydrogen Bonds• The bases attract each

other because of hydrogen bonds.

• Hydrogen bonds are weak but there are millions and millions of them in a single molecule of DNA.

• The bonds between cytosine and guanine are shown here with dotted lines

16

Hydrogen Bonds, Hydrogen Bonds, cont.• When making

hydrogen bonds, cytosine always pairs up with guanine

• Adenine always pairs up with thymine

• Adenine is bonded to thymine here

C

C

CC

N

N

N

N

N

C

C

C

C

C

N

N

OO

OO

C

17

18

Discovery of DNA Discovery of DNA StructureStructure

• Erwin Chargaff showed the amounts of the four bases on DNA (A,T,C,G)

• In a body or somatic cell: A = 30.3% T = 30.3% G = 19.5% C = 19.9%

Adapted from cmassengale

19

Chargaff’s Rule:Chargaff’s Rule:

• Adenine and Thymine always join together

A T

• Cytosine and Guanine always join together

C G

T

A

G

C

Rosalind Franklin

20

21

Watson & Crick’s Model

22

EXIT SLIP!

http://www.youtube.com/watch?v=FUA6_Ucw3i4&feature=related

• Hip Hip Hooray• Blame it on the DNA

23

24

NucleotidesNucleotides

C C

C

OPhosphate

O

CC

O -P OO

O

O -P OO

O

O -P OO

O One deoxyribose together with its phosphate and

base make a nucleotide.

Nitrogenous base

Deoxyribose

25

Two Kinds of Bases in DNA

• PyrimidinesPyrimidines are single ring are single ring basesbases..

• PurinesPurines are are double ring double ring bases.bases.

C

C

C

C

N

N

OO

N

CC

CC

N

N

N

N

N

C

26

Adenine and Guanine Adenine and Guanine are purinesare purines

• AdenineAdenine and and guanineguanine each have each have two rings of carbon and two rings of carbon and nitrogen atoms.nitrogen atoms.

C

C

C

C

N

N

N

AdenineAdenine N

N

C

C

C

C

C

N

NOO

N

GuanineGuanine N

N

C

27

Thymine and Cytosine Thymine and Cytosine are pyrimidinesare pyrimidines

• Thymine and cytosine each have one Thymine and cytosine each have one ring of carbon and nitrogen atoms.ring of carbon and nitrogen atoms.

C

C

C

C

N

N

OO

N

cytosinecytosine

C

C

C

C

N

N

OO

OO

thyminethymine

C

DNA Replication

• http://www.johnkyrk.com/DNAreplication.html

28