0.001

0.01

0.1

1

0 24 48 72 96 120 144 168 192 216 240

Fig. S1. Growth curve of A. pernix K1 in different degree of aeration. Cells were grown in medium 3ST, with shaking at 120 rpm (filled circles and line), or without shaking (open circles and dotted line). The experiment was performed in duplicates. Standard deviation is represented in bars.

hours

OD

(60

0 nm

)

PCRseq1 1 TGCAGCGCAGACTAAGATCAATATTCAAACCCTCGACCGCCGCCTCAGAGAGTTCGCCCA

ApeK1 580187 TGCAGCGCAGACTAAGATCAATATTCAAACCCTCGACCGCCGCCTCAGAGAGTTCGCCCA

************************************************************

PCRseq1 61 GCTACGCCTCCTAGAGAGGAGGGAGATAAAGAAGGATGGCAGGCGGAGGGCGAGAACCAT

ApeK1 580247 GCTACGCCTCCTAGAGAGGAGGGAGATAAAGAAGGATGGCAGGCGGAGGGCGAGAACCAT

************************************************************

PCRseq1 121 CTTCTGCCTGCCCGAGGACCTCGCAAGCTTTTACCTAGACGCCGGGAGGGGGTGAGAATG

ApeK1 580307 CTTCTGCCTGCCCGAGGACCTCGCAAGCTTTTACCTAGACGCCGGGAGGGGGTGAGAATG

************************************************************

PCRseq1 181 GTGCTCAGCGTGAGCTTCACAGGCAGGATAAGCAAGCGTAGGGGGCAGGTGTTGGTGCTC

ApeK1 580427 GTGCTCAGCGTGAGCTTCACAGGCAGGATAAGCAAGCGTAGGGGGCAGGTGTTGGTGCTC

************************************************************

PCRseq1 241 CTCCCCCGCGACTACTGGCAGCTCTTCCAGGGGCTCAGGGGTAAGAAGGTGAGGGTTAGG

ApeK1 580427 CTCCCCCGCGACTACTGGCAGCTCTTCCAGGGGCTCAGGGGTAAGAAGGTGAGGGTTAGG

************************************************************

PCRseq1 301 GTCGGGGAGGTGGAGTACACTGGCAGGATCACGGTTATCGAGGAGAGAGTGTACGTCTCG

ApeK1 580487 GTCGGGGAGGTGGAGTACACTGGCAGGATCACGGTTATCGAGGAGAGAGTGTACGTCTCG

************************************************************

PCRseq1 361 CTTCCTTTTTCGGCTCTGGCCCTGAGGGAGCGTAGCAGGTACCACCTTATCCGCCTGGAG

ApeK1 580547 CTTCCTTTTTCGGCTCTGGCCCTGAGGGAGCGTAGCAGGTACCACCTTATCCGCCTGGAG

************************************************************

PCRseq1 421 GTGTAGGTTATGAGAGTGGTTGAGAGGCTTGGAGCTCCACACAGCTATACGCCTGCTAAA

ApeK1 580607 GTGTAGGTTATGAGAGTGGTTGAGAGGCTTGGAGCTCCACACAGCTATACGCCTGCTAAA

************************************************************

PCRseq1 481 CACGCCTACACCGGGCCCCGCCCTTACACGGCGGAGGTCCGGGGTTCAAATCCCCGCGGG

ApeK1 580667 CACGCCTACACCGGGCCCCGCCCTTACACGGCGGAGGTCCGGGGTTCAAATCCCCGCGGG

************************************************************

PCRseq1 541 CCCACCA

ApeK1 580727 CCCACCA

*******

PCRseq1 498 CCGCCCTTACACGGCGGAGGTCCGGGGTTCAAATCCCCGCGGGCCCACCAGCGCATCATA

ApeK1 542635 CCGCCCTTACACGGCGGAGGTCCGGGGTTCAAATCCCCGCGGGCCCACCAGCGCATCATA

************************************************************

PCRseq1 558 CA

ApeK1 542695 CA

FIG. S2. Nucleotide sequence alignment of the PCR amplified fragment of APSV1 genome containing attP site with A. pernix K1 chromosome. PCR primers V1-F and V1-R were used.

FIG. S3. Transmission electron micrograph of APOV2 virions produced by A. pernix YKP1 strain. Negatively stained with 2 % uranil acetate. Scale bar, 100 nm.

PCRseq2 1 TTGGTGCCTAAACCCTTGTACGTGTACGTCCACACCGTCAAGAGCAAGGGCAGGATATAC

ApeK1 491733 TTGGTGCCTAAACCCTTGTACGTGTACGTCCACACCGTCAAGAGCAAGGGCAGGATATAC

************************************************************

PCRseq2 61 CAGTACCTCGTCGTAGAGGAGTACCTCGGCCAAGGCAGGCGTAGAACCATCCTCAGGATG

ApeK1 491793 CAGTACCTCGTCGTAGAGGAGTACCTCGGCCAAGGCAGGCGTAGAACCATCCTCAGGATG

************************************************************

PCRseq2 121 CGCCTCGAAGAAGCCGTAAGGAAGCTGCTAAACAATGAAAAGAAGGACAGCGCCGAAACC

ApeK1 491853 CGCCTCGAAGAAGCCGTAAGGAAGCTGCTAAACAATGAAAAGAAGGACAGCGCCGAAACC

************************************************************

PCRseq2 181 GCAGGGTGGTGCGGGGGGTGGGATTTGAACCCACGCAGGCCTACGCCAACGGGTCTTAAG

ApeK1 491913 GCAGGGTGGTGCGGGGGGTGGGATTTGAACCCACGCAGGCCTACGCCAACGGGTCTTAAG

************************************************************

PCRseq2 241 CCCGCCCCCT

ApeK1 491973 CCCGCCCCCT

**********

PCRseq2 186 GTGGTGCGGGGGGTGGGATTTGAACCCACGCAGGCCTACGCCAACGGGTCTTAAGCCCGC

ApeK1 478149 GTGGTGCGGGGGGTGGGATTTGAACCCACGCAGGCCTACGCCAACGGGTCTTAAGCCCGC

************************************************************

PCRseq2 246 CCCCTCTAAGCCATTCTCATCGATGGTAATTGAAAAGAGAGATTCGGGTGATGGAGAATC

ApeK1 478209 CCCCTCTAAGCCATTCTCATCGATGGTAATTGAAAAGAGAGATTCGGGTGATGGAGAATC

************************************************************

PCRseq2 306 GGAGCCTAGTTTTAAGCAGGATGGAGGGTTGATTGTTTCTGAAACTCTGGCTTCCAGGTT

ApeK1 478269 GGAGCCTAGTTTTAAGCAGGATGGAGGGTTGATTGTTTCTGAAACTCTGGCTTCCAGGTT

************************************************************

PCRseq2 366 TCTCGAGTG

ApeK1 478329 TCTCGAGTG

*********

FIG. S4. Nucleotide sequence alignment of the PCR amplified fragment of APOV1 genome containing attP site with A. pernix K1 chromosome. PCR primers V2-F and V2-R were used.

Table S1. Summary of predicted ORFs in the APSV1 genome

ORF strand Promotor RBS distance from

upstream ORF

start

codon

termination

codon

1-83 + AAAATA (-50) GAGGTGA (7) -3 ATG TAG

2-129 + TCTAA (-32) GGTGG (-6) 139 TTG TAG

3-326 + - AGGGTG (9) 1 ATG TAG

4-235 - ATATTATATA (35) GAGTGG (-4) 59 TTG TAA

5-322 + TATTA (-65) AGAGG (-7) 382 TTG TAA

6-63 + TTTAAA (-41) GGAGG (-21) 221 GTG TAG

7-264 - TTATTATATA (-31) GACA (-19) 142 ATG TAG

8-91 + TTATTATA (-78) GTAGGGTG (-6) 352 GTG TAG

9-159 + - GAGGAAG (-17) -20 ATG TAG

10-319 + - TAGGG (-4) 6 TTG TAG

11-134 + - GGGTA (-5) 4 TTG TAG

12-290 + - GAGGTG (-8) -11 GTG TAG

13-38 - - GGTGA (-14) -3 ATG TAA

14-221 + TTATTT (-18) GGGGGT (-12) 26 ATG TAG

15-181 + - GTAGGGG (-11) 198 GTG TGA

16-414 + - TGAGG (-13) 37 ATG TAG

17-96 + - ATTGGGG (-6) 25 ATG TAG

18-929 + TACATAATAAT (-44) GAGGAGTA (-14) 4 GTG TAG

19-123 + - AGTGTGGGGGG (-9) 35 TTG TGA

20-96 + - GGGATGGA (-4) 0 ATG TAG

21-105 + ATATATAT (-19) GGAGG (11) 31 ATG TAG

22-88 + ATATATA (-17) GGTGAA (-7) 28 TTG TAG

23-424 + - GGGTG (-7) 51 TTG TGA

24-73 + - AGGGGT (-12) 0 ATG TAG

25-282 + - AGGGTGG (-5) 22 GTG TAG

26-534 + - GGTGGGG (-5) 88 GTG TAG

27-168 + TAATAA (-54) GAGTG (-5) 274 ATG TAG

28-338 + - GGTGAGG (-5) -17 ATG TAG

29-106 + TATAAA (-27) GTGGG (-5) 77 TTG TAG

30-135 + - GTGGTG (-10) 45 TTG TGA

31-111 + - GGGGTG (-4) 30 ATG TAA

32-325 + ATTTTAT (-18) GGGGAGGG (-5) 94 TTG TAG

33-85 + TTAAAA (-41) GGAGGAGG (6) -7 TTG TAA

34-63 + ATTTTCTCTAA (-77) GGAAG (-5) 166 GTG TAG

35-155 + TTTAATAT (-16) AGGTGATGA (-5) 173 TTG TAG

36-351 - TTAAATT (-24) GAATG (-5) -34 ATG TGA

37-66 + TATATT (-30) GGTGGA (-6) 467 ATG TGA

38-469 + ATTAA (-25) GGAGGTG (-8) -3 TTG TAG

39-350 - ATAAAAT (-27) AAGGTG (-8) 2 TTG TGA

40-223 + TTTTTAA (-29) GGTGT (-7) 85 GTG TAA

41-152 + TATATTA (-65) GAAAG (-8) 74 ATG TGA

42-479 + - GGGAG (-4) 0 ATG TAG

43-534 + - GGGTGGA (-6) 3 GTG TAG

44-94 - TATAA (-35) GGGAGA (-5) 69 ATG TAG

45-81 + TTTATTA (-48) GGTGA (-4) 139 GTG TGA

46-83 + - GGGTGA (-7) 7 GTG TAG

47-142 + - AGGAGG (-11) 0 GTG TGA

48-79 + - GAGGGTGTGA (-8) 8 GTG TAG

49-93 + - AGGGGTGA (-7) 13 ATG TAA

50-76 + - GAGGTGGAGA (-5) -10 GTG TAG

51-108 + - AGGGGGTGG (-7) 14 TTG TGA

52-82 + - GGAGGGGG (-6) 3 ATG TAG

53-521 + - GGAGGTG (-7) 4 ATG TGA

TABLE S2. Significant database matches of 3 clustered putative genes of APSV1 1st match 2nd match 3rd match 4th match 5th match

ORF38-470 Thermoproteus neutrophilus Pyrobaculum aerophilum Thermosphaera aggregans Micromonospora aurantiaca Acinetobacter sp.

3e-174a 8e-164 6e-133 2e-31 4e-29

ORF39-351 Thermoproteus neutrophilus Pyrobaculum aerophilum

1e-110 5e-97

ORF40-224 Pyrobaculum aerophilum Thermoproteus neutrophilus Caldicellulosiruptor kristjanssonii Thermosphaera aggregans Thermofilum pendens

1e-75 1e-48 3e-45 2e-40 3e-37

a. The numbers below the organism names indicate E-values.

Supplimentary Table S3. Summary of predicted ORFs in the APOV1 genome

ORF strand Promotor RBS distance from

upstream ORF

start

codon

termination

codon

1-128 - - GAGAGGCGGG (-7) 298 ATG TAA

2-89 - - AGGGG (-8) -11 TTG TAA

3-113 - AATAATAA (-15) GAGGGA (-3) 4 GTG TGA

4-81 - TTAAATATTT (-41) GAGGTG (-7) 7 GTG TGA

5-219 + ATTTTTAAA (-35) AAGAA(-13) 122 GTG TAA

6-94 - ATATAA (-39) AGAGAG (-4) -88 TTG TAA

7-57 - TCTATTT (-56) AGAGTGG (-9) 349 ATG TGA

8-107 - TTAATTAA (-36) GGTGG (-7) 106 TTG TAA

9-678 - ATATTTTAAA (-13) GATG (-5) 53 TTG TAG

10-284 - TATACT (-84) GGGACA (-15) 91 GTG TAA

11-222 - - AGAGGGGG (-6) 154 ATG TAG

12-85 - TAAACAATTT (-36) AGAGG (-9) 22 TTG TAA

13-94 + TCTTTTA (-30) AGGTGGGTG (-11) 89 ATG TAG

14-121 - - GGTGGGA(-3) 87 ATG TAA

15-259 - AATACA (-15) TGGGGG (-6) 11 TTG TAG

16-391 - TAATT (-21) GGAA (-3) 65 ATG TAG

17-201 + AATCAACTA (-20) GGAG (-3) 56 ATG TAG

18-132 + - GGGTGG (-6) -7 TTG TAG

19-247 + - GAGGTGGA (-5) 17 ATG TAG

20-100 + - GAGGATGA (-4) -14 GTG TAA

21-399 + TTAAATCAT (-41) ATGGAGA (-4) 15 ATG TAA