()

()

(22 )

( 1 Angsana New 16 point)

20 .. 2549

(())(22 )

( 1 Angsana New 16 point)

()

1

1.1 1

1.2 2

1.3 2

1.4 2

1.5 2

1.6 3

1.7 3

2

2.1

2.1.1 4

2.1.2 5

2.1.3 6

2.2

2.2.1 7

2.2.2 7

()(22 )

( 1 Angsana New 16 point)

2.2.4 9

2.2.5 11

2.2.6 13

2.2.7 15

2.2.8 15

2.3 16

2.4 18

2.4.1 19

2.4.2 19

2.4.3 19

2.5 20

2.5.1 21

2.5.2 21

2.5.3 22

3

3.1 24

3.1.1 24

3.1.2 25

3.1.3 25

3.2 25

3.2.1 25

3.2.2

26

()

( 1 Angsana New 16 point)

3.2.4

27

3.2.5

27

3.3 28

4

4.1

29

4.2

31

4.3

34

4.4

36

5 38

40

46

47

48

61

(())(22 )

( 1 Angsana New 16 point)

2.1 5

2.2 17

2.3 17

2.4 19

2.5 20

2.6 100 21

3.1 26

4.1 L*a* b* 30

40 50 60 70

12 30

4.2 30

L*a*b*

60 9 12 15 33

4.3 30

L*a* b*

60 12 7.5 36

4.4

60 12 36

4.5

36

4.6 37

(())(22 )

( 1 Angsana New 16 point)

2.1 4

2.2 8

2.3 9

2.4 10

2.5 18

4.1

30

4.2

31

4.3 35

(()())

( 1 16 point)

L* 0 () 100 ()

a* a* a*

b* b* b*

kcal

( Angsana New 16 point ) ( 1 2 16 point)

1 (22 )

(22 )

( 1 16 point)

( 1/2 ( 8 piont))1.1 (18 )

. .

( 1/2 ( 8 piont))

1.2

1.2.1

(tray dryer)

1.2.2

1.3

1.3.1 . 12

1.4

1.6.1

1.5

1 .. 2545 - .. 2546

1.6

1.7 () , , , ,

( 2 2 Angsana New 16 point)

2

( 1 16 point) ( 1/2 ( 8 piont))2.1

2.1.1

Pleurotus cystidiosus (Fr.) Quel. Abalone mushroom Agaricaceae 2 5 3 8 1 18 20 1 2 0.5 1 48 () ( , 2532; , 2530)

2.1.1.1 2.1.1

1) 2.1.1.1

( 3 2 Angsana New 16 point)

3

( 1 16 point)

( 1/2 ( 8 piont))3.1

3.1.1 ( )

Acetic acid (glacial)(CH3COOH)MERCK, 64271 Darmstadt , Germany

AcrylamideGibco BRL, Life Technologies

Adjuvant complete freundDifco Laboratorie. Detrolt Ml. 48232-7058 U.S.A.

BluPrint Fast-PAGE stainGibco BRL, Life Technologies

Bovine serum albumin(BSA)Fluka. Chemie AG,CH-9470 Buchs Switzerland

Calcium chloride(CaCl2)BDH Chemicals Ltd. Poole England

Cupric sulfate pentahydrate(CuSO4.5H2O)Fluka. Chemie AG,CH-9470 Buchs Switzerland

3,3 Diaminobenzidine tetrahydrochloride (DAB)BDH Chemicals Ltd. Poole England

(NH2)2C6H3,C6H3(NH2)2,4HCl,H2O

Di-sodium hydrogen phosphate(Na2HPO4)Fluka. Chemie AG,CH-9470 Buchs Switzerland

D-Glucose anhydrous(C6H12O6)AMRESCO Solon, OHIO

D-MEM/F-12 Gibco BRL, Life Technologies

Eggs yolkSigma Chemical CO.PO. Cox 14508, St. Louis, MO 63178 U.S.A.

Foetal Bovine SerumGibco BRL, Life Technologies

Glyceral anhydrousFluka. Chemie AG,CH-9470 Buchs Switzerland

GlycineGibco BRL, Life Technologies

Goat Anti-Mouse IgG(H&L) peroxidase labeledHyClone Logan,Utah 1-800-HyClone

HAT Supplement 100xGibco BRL, Life Technologies

Hydrogen peroxide(H2O2)CARLO ERBA REAGENTI s.n.l. montedision group.

L-glutamineSigma Chemical CO.PO. Cox 14508, St. Louis, MO 63178 U.S.A.

Magnesium sulfate HeptahydrateFluka. Chemie AG,CH-9470 Buchs Switzerland

N',N'-MethylenebisacrylamideGibco BRL, Life Technologies

N',N',N',N'-Tetramethyl-Ethylenediamine (TEMED)Sigma Chemical CO.PO. Cox 14508, St. Louis, MO 63178 U.S.A.

Penicillin G SodiumM&H Manufacturing CO., LTD.

Potassium dihydrogen phosphate(KH2PO4)Fluka. Chemie AG,CH-9470 Buchs Switzerland

Potassium chloride(KCl)BDH Chemicals Ltd. Poole England

StreptomycinM&H Manufacturing CO., LTD

Sodium acetate(C2H3O2Na)Fluka. Chemie AG,CH-9470 Buchs Switzerland

Sodium carbonate(Na2CO3)Fluka. Chemie AG,CH-9470 Buchs Switzerland

Sodium chloride(NaCl)Fluka. Chemie AG,CH-9471 Buchs Switzerland

Sodium dihydrogen phosphate (NaH2PO4)Fluka. Chemie AG,CH-9470 Buchs Switzerland

Sodium hydroxide (NaOH)Eka Nobel AB,S-445 80 Buhus

3.1.2 ( )

PHARMACIA BIOTECH

(Electroph wick)

Hoefer Phamacia Biotech Inc.

(Nitrocell 33 CMX3M ROLL) Sanfancisco, California, U.S.A.

1 TERUMO

(Syringe with needle U-100 insulin)

27x1/2" 25x1"TERUMO

(Needle 27x1/2" and 25x1")

50 250 NUNCLON DELTA

(50 ml and 250 ml Tissue Culture Flask)

ELISANUNCLON DELTA

(Nunc-Immuno Plate MexiSorp)

96 NUNCLON DELTA

(96 wells Tissue Culture dish)

24 NUNCLON DELTA

(24 wells Tissue Culture dish)

BIOHIT OY. FINLAND

(BIOHIT PROLINE Pipette)

BIOHIT OY. FINLAND

(BIOHIT PROLINE Multichannel Pipette)

INTEGRA BIOSCIENCES S.A.

(PIPETBOY)

NALGENE U.S.A.

(Cryotube)

50 NALGENE U.S.A.

3.2 ()

NIKON, JAPAN

(Light microscorp)

NIKON, JAPAN

(Phase contrast microscope)

OLYMPUS

(Inverted microscope)

SCIENTIFIC INDUSTRIES, INC.,

(Vertex mixer) BOHEMIA N.Y. 11716 U.S.A

NEW BRUNSWICK SCIENTIFIC

(Controller Environment Incubator Shaker)CO,INC EDISON, N.J., U.S.A

BIO-RAD LABORATORIES

(MODEL 200/2.0 Power supply)

3 SARTORIUS LABORATRY

HIRAYAMA, JAPAN

(Autoclave)

THERMOMIX UB

(Cooling)

40 25 DAMON/IEC DIVISION

(IEC HN-S Centrifuge)

DU PONT INSTRUMENTS

(Sorval RC-5B Refrigerated Superspeed Centrifuge)

SHELTON SCIENTIFIC

(Mini Centrifuge VSMC-B)

TOKYO RIKAKIKAI CO,LTD

(Freeze Dryer FD-1)JAPAN

-SUNTEX, U.S.A.

(pH/mV/temp,meter SP-701)

CECIL

(CE 2031 2000 SERIES Spectrophotometer)

ELISASPECTRA COUNT PACKARD

(ELISA reader)A CANBERRA COMPANY

FROMA-GERATETECHNINK

(Hot plate)

BIO-RAD LABORATORIES

(Mini-PROTEIN(II Dual Slab Cell)

LKB BROMMA

(2117 Multiphor II Eletrophoresis Unit)

PHARMACIA BIOTECH

(NOVABLOT ELECTRODE CATHODE AND ANODE)

NEWLAB FUME CUPBOARD

(Hood)

NUARIE, U.S.A.

(Biologycal safety cabinets classII type A/B3)

PRECISION

(Incubator)

SHELLAB

(MODEL TC 2323 CO2 INCUBATOR)

MITSUBISHI,JAPAN

(J- elegance MR-F36C-GY)

( 1/2 ( 8 point))

3.3

3.3.1 Bacilllus thuringiensis israelensis (Bti)

3.3.1.1 Bti Hurley (1987)

( 4 2 16 point)

4

( 1 16 point)

4.1

4 40 50 60 70 12 L*a*b* 30 4.1 70 30 60 (p 0.05) 50 40 60 70 60 70 40 50 60 4 4.1 4.2 60 60

4.2

() 4.1 L*a*b*

30 40

50 60 70 12

()

30

L*

a*

b*

40

50

60

70

36.69a 0.84

33.06a 4.32

23.72b 3.68

23.71b 3.79

31.81b0.11

33.94a0.10

34.82a0.06

32.21ab0.07

12.41b0.05

13.54a0.07

14.11a0.10

13.95a0.06

15.47a 0.15

16.76a 0.12

16.22a 0.15

13.91b0.07

59.33c 24.20

108.86b 27.66

124.98a 20.72

122.61a 11.54

(p 0.05)

40 50 60 70

()

40 50 60 70

()

4.1

(1)

(2) 80 1:3

( 5 2 Angsana New 16 point)

5

( 1/2 ( 8 piont))( 1 16 point)

Marimuthu et al. (1989) 50 , (2544) 40 70 (2546) 40 70 40 70 0.71 (Bobi, Bauman and uri, 2002) 12 60 30 23.72 124.98 L*a*b* 34.82 14.11 16.22 60 3 24 (2546) 9 0.600 (Adam and Moss, 2000) 9 12 15

( 2 Angsana New 16 point)

(22 )( 1 16 point)

, . (2535). . : . ( 1.5 cm)

. (2536). :

.

.

. (2544). 2544. .

(). .

11:14 23.

. (2532). . 3. :

.

. (2544). .

.

. (2532). . : .

. (2536). . 4. : .

. (2542). . 7. : .

, . (2526). . 6(6) : 20 22.

. (2543). . : .

. (2525). . : .

. (2546). . .

. (2544). .

5 9 .. 2544 ( 1 35). :

.

. (2000). . Online. Available http://

www.rdi.ku.ac.th/news_announce/old_news/knowledge/cancer/index_cances.html.

. (2542). . : .

Adam, M. R. and Moss, M. O. (2000). Food Microbiology. 2nd ed. Cambridge : Royal Society

of Chemistry.

Barbosa-Canovas, G. V. and Vega-Mercado, H. (1996). Dehydration of Foods. New York :

Chapman & Hall.

Codex Alimentarius. (1981). Codex general standard for edible funri and fungus products

Codex Stan 38. Rome : Author.

IFST. (1993). Shelflife of foods Guidelines for its determination and prediction. United

Kingdom : Author.

Khalloufi, S., Giasson, J. and Ratti, C. (2000). Water activity of freeze dried mushrooms and

berries. Canadian Agricultural Engineering 42(1) : 7.1 7.13.

Marcotte, M. (2001). Effect of irradiation on spices, herbs and seasonings comparison with

ethylene oxide fumigation Online. Available http://www. Foodirradiation.com/Spices.htm.

Marimuthu, T., Krishanamoorthy, A. S., Sivanprakasam, K. and Jayarajan, R. (1989). Oyster

mushroom production. Coimbatore : Tamiknadu University.

Mazza, G. and Leamaguer, M. (1980). Dehy dration of Onion : Some theoretical and Practical

Consideration. Journal of Food Technology. 15 : 181 191.

National Food Authority. (1995). Composition of foods, Australia volume 7 : Ethic foods amino acids update. Australia : Author.

Peter, J., Robert, K. and Hunter, A. (n.d.). Food analysis. 3rd ed. Hawkesbury: University of

Western Sydney.

Rahart, J. (1999). Preserving harvested mushrooms. MycoWest News Online. Available

http:/swcp.com/mycowest/books/p-9903jr.htm.

Siripongvuttikorn, S., Huang, H. W. and Thammaratwasik, P. (2002). Antibacterial effect and antioxidant content of Tom Yam Online. Available http://ift.confex.com/

( 2 Angsana New 16 point)

(22 )( 1 16 point)

1 : (18 )

1.1 Egg Yolk agar medium (EYA) (16 )

Tryptone10.0

Na2HPO4 5.0

KH2PO4 1.0

NaCl 2.0

MgSO4.7H2O 0.1

Glucose 2.0

Egg yolk 10.0

1000

Egg yolk 1000 pH 7.6 agar 13 15 121C 20 40-50C Egg yolk

1.2 Bacillus thuringiensis medium (Bt medium)

Glucose3.0

(NH4)2SO42.0

Yeast extract2.0

K2HPO40.5

MgSO4.7H2O0.2

CaCl20.08

MnSO4.H2O0.05

1000

( 2 Angsana New 16 point)

( 1 16 point)

14 .. 2523 () 2536 2539

4 .. 2523 () 2536 2539

24 .. 2523 () 2536 2539