© spex sampleprep, inc. 2011. the basics designed originally for pioneer hybrid international, a...

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© SPEX SamplePrep, Inc. 2011

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© SPEX SamplePrep, Inc. 2011

© SPEX SamplePrep, Inc. 2011

The Basics

• Designed originally for Pioneer Hybrid International, a leading company in genetic crop science and now a division of DuPont

• The Geno/Grinder® provides efficient cell disruption of plant and animal tissues for the isolation of nucleic acids (RNA/DNA), enzymes, yeast and proteins.

© SPEX SamplePrep, Inc. 2011

Features & Benefits – Clamp Design

• Unique, true vertical action ensures complete sample processing

• Standard Clamp accepts two 96-well titer plates & various vials from 0.6mL to 50mL

• Large Clamp doubles throughput from 2 titer plates up to 4 and holds up to sixteen 50mL vials

• Full range of cryogenic accessories for temperature sensitive samples

Patented

© SPEX SamplePrep, Inc. 2011

Who Can Benefit from a Geno/Grinder®?

• Institutions using high-throughput DNA sample processing

• Biotech / Pharmaceutical• Agriculture/Crop Sciences• Public Health Labs• Colleges/Universities

© SPEX SamplePrep, Inc. 2011

Samples & Applications

Rapid preparation of multiple samples for PCR sampling

Fast disruption to allow extraction of Nucleic acids, RNA/DNA

Lysing of yeast, bacterial cellsPesticide AnalysisQuEChERS Method

© SPEX SamplePrep, Inc. 2011

QuEChERS Method

• Plant tissue from fruits and vegetables are homogenized using the Geno/Grinder®

• QuEChERS reagents are added for extraction, eliminating cross contamination

• Can be homogenized at room temperature or with Kryo-Tech® accessories

• Used by FDA

Quickasy

eapffectiveuggedafe

EChERS

© SPEX SamplePrep, Inc. 2011

Geno/Grinder Accessories

© SPEX SamplePrep, Inc. 2011

Geno/Grinder® Accessories

Titer Plates & Cap MatsVials & Vial Sets

Kryo-Tech®Racks & Holders

Grinding Media

© SPEX SamplePrep, Inc. 2011

Titer Plates & Vials

• Titer Plates– 24-Well Square– 48-Well Rectangle– 96-Well Square– 96-Well Round

• Vials– Available sizes: 2mL, 5mL, 15mL, 50mL– NEW! 2mL pre-filled vials

• Zirconium, Silica Beads• Stainless Steel balls• Available in packs of 50 or 100

– NEW! Cryogenic Grinding Vial (P/N: 2255)• Stainless steel vial set specifically designed for cryogenic grinding in the

Geno/Grinder®

NEW!

NEW!

© SPEX SamplePrep, Inc. 2011

Geno/Grinder® Accessories

Titer Plates & Cap MatsVials & Vial Sets

Kryo-Tech®Racks & Holders

Grinding Media

© SPEX SamplePrep, Inc. 2011

Racks, Holders & Grinding Media

• Racks & Holders– Available for 2mL, 5mL, 15mL & 50mL– Durable, Sturdy Polyethylene material

• Grinding Media– Stainless Steel Grinding balls w/ dispenser– Silica & Zirconium grinding beads– Ceramic Grinding Media• Pesticide Extraction

© SPEX SamplePrep, Inc. 2011

Geno/Grinder® Accessories

Titer Plates & Cap MatsVials & Vial Sets

Kryo-Tech®Racks & Holders

Grinding Media

© SPEX SamplePrep, Inc. 2011

Kryo-Tech® Accessories

• 2600 Cryo-Station– Holding station for samples

which must be kept chilled to preserve RNA for extraction

• Cryo-Blocks & Holders– Maintains tissue samples at

sub-zero temperatures during homogenization process

– 2650 Cryo-Plate for Titer Plates

– Available for all SPEX SamplePrep vial sizes

© SPEX SamplePrep, Inc. 2011

Application Notes & Resources

© SPEX SamplePrep, Inc. 2011

Application Notes

• Extensive library of Geno/Grinder® applications

Examples• SP025 – Quick DNA Extraction from Rice

Seed (Wet)• SP024 – Analysis of Pesticides in Fruit and

Vegetable Products using a Standard QuEChERS Method and a Modified Method Involving the Geno/Grinder®

• SP018 – High-Throughput Disruption of Yeast in a 96-Well Format

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