© spex sampleprep, inc. 2011. the basics designed originally for pioneer hybrid international, a...
TRANSCRIPT
© SPEX SamplePrep, Inc. 2011
The Basics
• Designed originally for Pioneer Hybrid International, a leading company in genetic crop science and now a division of DuPont
• The Geno/Grinder® provides efficient cell disruption of plant and animal tissues for the isolation of nucleic acids (RNA/DNA), enzymes, yeast and proteins.
© SPEX SamplePrep, Inc. 2011
Features & Benefits – Clamp Design
• Unique, true vertical action ensures complete sample processing
• Standard Clamp accepts two 96-well titer plates & various vials from 0.6mL to 50mL
• Large Clamp doubles throughput from 2 titer plates up to 4 and holds up to sixteen 50mL vials
• Full range of cryogenic accessories for temperature sensitive samples
Patented
© SPEX SamplePrep, Inc. 2011
Who Can Benefit from a Geno/Grinder®?
• Institutions using high-throughput DNA sample processing
• Biotech / Pharmaceutical• Agriculture/Crop Sciences• Public Health Labs• Colleges/Universities
© SPEX SamplePrep, Inc. 2011
Samples & Applications
Rapid preparation of multiple samples for PCR sampling
Fast disruption to allow extraction of Nucleic acids, RNA/DNA
Lysing of yeast, bacterial cellsPesticide AnalysisQuEChERS Method
© SPEX SamplePrep, Inc. 2011
QuEChERS Method
• Plant tissue from fruits and vegetables are homogenized using the Geno/Grinder®
• QuEChERS reagents are added for extraction, eliminating cross contamination
• Can be homogenized at room temperature or with Kryo-Tech® accessories
• Used by FDA
Quickasy
eapffectiveuggedafe
EChERS
© SPEX SamplePrep, Inc. 2011
Geno/Grinder® Accessories
Titer Plates & Cap MatsVials & Vial Sets
Kryo-Tech®Racks & Holders
Grinding Media
© SPEX SamplePrep, Inc. 2011
Titer Plates & Vials
• Titer Plates– 24-Well Square– 48-Well Rectangle– 96-Well Square– 96-Well Round
• Vials– Available sizes: 2mL, 5mL, 15mL, 50mL– NEW! 2mL pre-filled vials
• Zirconium, Silica Beads• Stainless Steel balls• Available in packs of 50 or 100
– NEW! Cryogenic Grinding Vial (P/N: 2255)• Stainless steel vial set specifically designed for cryogenic grinding in the
Geno/Grinder®
NEW!
NEW!
© SPEX SamplePrep, Inc. 2011
Geno/Grinder® Accessories
Titer Plates & Cap MatsVials & Vial Sets
Kryo-Tech®Racks & Holders
Grinding Media
© SPEX SamplePrep, Inc. 2011
Racks, Holders & Grinding Media
• Racks & Holders– Available for 2mL, 5mL, 15mL & 50mL– Durable, Sturdy Polyethylene material
• Grinding Media– Stainless Steel Grinding balls w/ dispenser– Silica & Zirconium grinding beads– Ceramic Grinding Media• Pesticide Extraction
© SPEX SamplePrep, Inc. 2011
Geno/Grinder® Accessories
Titer Plates & Cap MatsVials & Vial Sets
Kryo-Tech®Racks & Holders
Grinding Media
© SPEX SamplePrep, Inc. 2011
Kryo-Tech® Accessories
• 2600 Cryo-Station– Holding station for samples
which must be kept chilled to preserve RNA for extraction
• Cryo-Blocks & Holders– Maintains tissue samples at
sub-zero temperatures during homogenization process
– 2650 Cryo-Plate for Titer Plates
– Available for all SPEX SamplePrep vial sizes
© SPEX SamplePrep, Inc. 2011
Application Notes
• Extensive library of Geno/Grinder® applications
Examples• SP025 – Quick DNA Extraction from Rice
Seed (Wet)• SP024 – Analysis of Pesticides in Fruit and
Vegetable Products using a Standard QuEChERS Method and a Modified Method Involving the Geno/Grinder®
• SP018 – High-Throughput Disruption of Yeast in a 96-Well Format
Also Available:
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© SPEX SamplePrep, Inc. 2011
Product Demonstration Videos