+ production of engineered long-life and male-sterile pelargonium plants
TRANSCRIPT
+
Production of Engineered long-life and male-sterile Pelargonium
plants
+ Key Words
-- Pelargonium zonale and Pelargonium peltatum
-- pSAG12 and PsEND1 promoter
-- Engineered long-lived and male sterile plants
-- ipt gene: expressed in long-living plants
-- Barnase: gene expressed in pollen free plants
-- Agrobacterium tumefaciens
-- Delayed senescence
-- Anther ablation: abnormalities in anther production
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+
Background
-- Flowering plant
-- Grown in Europe since 17th century
-- More than 200 species of Pelargonium
--Cultivars: P. zonale and P. peltatum is the most popular.
-- Species of Pelargonium are considered important in:
• Market of ornamental plants• Perfume industry
+
Pelargonium Breeds
Background
+Pelargonium breeders produce new flower colors, shapes and market quality plants via gene transfer
Long-living plants
Allergen-Free house plants
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+-Agrobacterium tumefaciens
-- Crown Gall Disease
-- Important in gene transfer
Plant Manufacturers
+-- In vitro regeneration system
-- Pelargonium zonale explant used as a starting materials.
-P. zonale – organogenesis - shoots
-Two new traits in P. zonale-Long-life plants- Male sterility
-Promoter used:-pSAG12 – Senescence-specific gene promoter-PsEND1 promoter – Anther specific promoter
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Regeneration of plants
+ Plant senescence-- Study of aging in plants
-- pSAG12 promoter fuses with ipt gene which has an ipt enzyme-- Produce more cytokinin
-- Delayed Senescence
+Influence of pSAG12:ipt
+Male Sterile Plants
-Why are they important?
- eliminates pollen allergens
- Prevents gene flow of GM material and related plant species.
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+Elimination of pollen allergens
Expression of barnase
gene
- Anther – specific PsEND1
promoter
-Specific ablation in
plants
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-Anther tissues are necessary
- No pollen grains in ablated anthers
- Barnase destroys cells for structural tissues
+Pelargonium zonale explant
Soak it in Agrobacterium- Plasmid with T-DNA region that
containsIpt and Barnase gene under the
control of their promoters
Plasmid leaves bacteria, goes into plant cells
DNA chromosome= tumors
Place into Media with Hormones
- Roots- Shoots
Transfer to a pot to fully grow
+RESULTS
pSAG12::ipt transgenic plants
Delayed leaf senscence
Increased branching
Smaller flowers
Intense coloration
Transgenic lines with PsEND1::barnase
No pollen grains in anther structures
ReferencesBioMed Central Limited (2012, August 29). No more sneezing: Allergen-free house plants. ScienceDaily. Retrieved November 18, 2012, from http://www.sciencedaily.com /releases/2012/08/120831083315.htm BioMed Central Limited (31 August 2012). Production of engineered long-life and male sterile Pelargonium plants. [Research Article] Available at: http://www.biomedcentral.com/1471-2229/12/156 . [Last Accessed 18 November 2012]. Biomedcentral.com (2012) www.biomedcentral.com - Figure. [online] Available at: http://www.biomedcentral.com/1471-2229/12/156/figure/F5 [Accessed: 27 Nov 2012]. Maria Lis-Balchin, (2007). Geranium and Pelargonium: The Genera Geranium and Pelargonium. 1st ed. New York: Taylor & Francis.
Mary Spink. Growing Pelargoniums. [ONLINE] Available at: : http://www.swanlandnurseries.co.uk/culturalnotes.asp. [Last Accessed 18 November 2012]. Scielo.cl (2007) Electronic Journal of Biotechnology - Influence of 2,4-D and BAP on callus growth and the subsequent regeneration of somatic embryos in long-term cultures of Pelargonium x domesticum cv. Madame Layal. [online] Available at: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000100007 [Accessed: 18 Nov 2012].