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0514-87970486

2011.1.4 415

1 415

: Zhu, AP (Zhu, Aiping); Lu, Y (Lu, Yan); Zhou, Y (Zhou, Yao); Dai, S (Dai, Sheng)

: Spherical N-carboxyethylchitosan/hydroxyapatite nanoparticles prepared by ionic diffusion process in a controlled manner

: JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE, 21 (12): 3095-3101 DEC 2010

: English

: Article

KeyWords Plus: CALCIUM-PHOSPHATE; CHITOSAN/HYDROXYAPATITE NANOCOMPOSITES; HYDROXYAPATITE NANOPARTICLES; COMPOSITE SCAFFOLDS; BONE; PASTE; BIOMATERIALS

: The nanocomposites containing hydroxyapatite (HA) and biomacromolecules have attracted considerable research interest in implants, tissue scaffolds and drug controlled delivery. In this study, the N-carboxyethylchitosan/hydroxyapatite (NCECS/HA) nanoparticles were prepared by the ionic diffusion process in a controlled manner. The crystallization, particle size, size distribution and aggregation morphology of the NCECS/HA nanocomposites were dependent on the mole ratio of the glucosamine unit in NCECS to the Ca2+. Fourier transform-infrared spectroscopic (FTIR) result indicated that there are chemical bonds formed between NCECS and HA. X-ray diffraction (XRD) analysis showed that the crystallization of HA in NCECS matrix was significantly retarded. Transmission electron microscopy (TEM) results revealed that NCECS/HA nanocomposites have the spherical morphology with the diameter ranging from 10 to 40 nm. The NCECS mineralization is driven by the self-assembly of NCECS and HA. These NCECS/HA nanocomposites have potential applications as the carrier for the controlled delivery of growth factors and drugs.

: [Zhu, Aiping; Lu, Yan; Zhou, Yao] Yangzhou Univ, Coll Chem & Chem Engn, Yangzhou 225002, Peoples R China; [Dai, Sheng] Univ Adelaide, Sch Chem Engn, Adelaide, SA 5005, Australia

: Zhu, AP, Yangzhou Univ, Coll Chem & Chem Engn, Yangzhou 225002, Peoples R China.

: [email protected]

:

National Natural Science Foundation of China

51073133

Natural & Scientific Grant of Jiangsu Province, China

BK2006072

08KJA430003

This research was supported by a National Natural Science Foundation of China (No. 51073133), a Natural & Scientific Grant of Jiangsu Province, Project No. BK2006072 (China), and a Project No. 08KJA430003 (China).

: 26

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: SPRINGER

: VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS

ISSN: 0957-4530

DOI: 10.1007/s10856-010-4157-7

29 : J MATER SCI-MATER MED

ISO : J. Mater. Sci.-Mater. Med.

: 7

: Engineering, Biomedical; Materials Science, Biomaterials

ISI : 685BW

: Qin, XY (Qin, Xuyin); Liu, Y (Liu, Yang); Mao, SJ (Mao, Shanjing); Li, TB (Li, Tianbi); Wu, HK (Wu, Hsinkan); Chu, CC (Chu, Chengcai); Wang, YP (Wang, Youping)

: Genetic transformation of lipid transfer protein encoding gene in Phalaenopsis amabilis to enhance cold resistance

: EUPHYTICA, 177 (1): 33-43 JAN 2011

: English

: Article

: Phalaenopsis amabilis; Agrobacterium tumefaciens-mediated transformation; LTP gene; Cold tolerance

KeyWords Plus: AGROBACTERIUM-TUMEFACIENS; MEDIATED TRANSFORMATION; INDUCIBLE GENES; ORCHID; PLANTS; SUPEROXIDE; EXPRESSION; MEMBRANES; PATHWAYS; PROLINE

: Embryogenic callus of Phalaenopsis amabilis derived from leaf tissue was cocultivated with Agrobacterium tumefaciens strain LBA4404 harboring a plant cloning vector. The vector carried the lipid transfer protein (LTP) encoding gene cloned from cold tolerant Brazilian upland rice cv. IAPAR 9. The highest transformation efficiency (12.16%) was obtained when 1-2 mm calli were infected and cocultivated with 0.4 (OD600) A. tumefaciens for 20 min. Transgene integration of kan-resistant plants was confirmed through polymerase chain reaction analysis and Southern hybridization. Four hundred seventy transgenic plants, each derived from an independent protocorm-like body, were obtained. The expression of rice cold-inducible LTP gene in transgenic P. amabilis improved its adaptive responses to cold stress. The examination of transgenic plants revealed that enhanced cold tolerance was most likely due to the increased accumulation of several compatible solutes such as total soluble sugars, proline, antioxidant superoxide dismutase, decreased accumulation of malondialdehyde, and maintained electrolytes within the membrane compared with controls.

: [Qin, Xuyin; Liu, Yang; Mao, Shanjing; Li, Tianbi; Wang, Youping] Yangzhou Univ, Coll Biosci & Biotechnol, Yangzhou 225009, Peoples R China; [Wu, Hsinkan] Yangping Hort Co Ltd, Yangzhou 225008, Peoples R China; [Chu, Chengcai] Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China

: Wang, YP, Yangzhou Univ, Coll Biosci & Biotechnol, Yangzhou 225009, Peoples R China.

: [email protected]

:

Chinese National Major Program of Transgenic Research

2009ZX08 004-002B

This work was funded by Chinese National Major Program of Transgenic Research (2009ZX08 004-002B).

: 40

: 0

: SPRINGER


ISSN: 0014-2336

DOI: 10.1007/s10681-010-0246-4

29 : EUPHYTICA

ISO : Euphytica

: 11

: Agronomy; Plant Sciences; Horticulture

ISI : 685TK

3 415

: Long, MA (Long Miao); Lempken, W (Lempken, Wolfgang)

: ON M-PERMUTABLE MAXIMAL SUBGROUPS OF SYLOW SUBGROUPS OF FINITE GROUPS

: COMMUNICATIONS IN ALGEBRA, 38 (10): 3649-3659 2010

: English

: Article

: Finite groups; M-permutable subgroups; Supersolvable groups; Sylow subgroups

: Let d be the smallest generator number of a finite p-group P, and let M-d(P) = {P-1, P-2,..., P-d) be a set of maximal subgroups of P such that boolean AND(d)(i=1) P-i = Phi(P). Then P is called M-permutable in a finite group G, if there exists a subgroup B of G such that G = PB and PiB < G for any P-i of M-d(P). In this article, we investigate the influence of M-permutability of some primary subgroups in finite groups. Some new results about supersolvable groups and formations are obtained.

: [Long Miao] Yangzhou Univ, Sch Math Sci, Yangzhou 225002, Peoples R China; Univ Duisburg Essen, Inst Expt Math, Essen, Germany

: Long, MA, Yangzhou Univ, Sch Math Sci, Yangzhou 225002, Peoples R China.

: [email protected]

:

NSFC

10901133

Qing Lan Project

Natural Science Fund for colleges and universities in Jiangsu Province

The first author is supported by the grant of NSFC (Grant #10901133) and sponsored by Qing Lan Project and Natural Science Fund for colleges and universities in Jiangsu Province.

: 16

: 0

: TAYLOR & FRANCIS INC

: 325 CHESTNUT ST, SUITE 800, PHILADELPHIA, PA 19106 USA

ISSN: 0092-7872

DOI: 10.1080/00927870903222533

29 : COMMUN ALGEBRA

ISO : Commun. Algebr.

: 11

: Mathematics

ISI : 685FA

4 415

: Wu, M (Wu, Min); Zhu, XS (Zhu, Xiashi)

: beta-Cyclodextrin cross-linked polymer as solid-phase extraction material coupled with the spectrophotometric method for the analysis of serum albumin

: SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, 77 (5): 1021-1024 DEC 2010

: English

: Article

: beta-Cyclodextrin cross-linked polymer; Solid-phase extraction; Bovine serum albumin; Phenylfluorone

KeyWords Plus: PROTEIN; SPECTROMETRY

: The beta-cyclodextrin cross-linked polymer (beta-CDCP) was synthesized and used as solid-phase extraction material to preconcentrate/analysis bovine serum albumin (BSA) coupled with UV-vis spectroscopy. The method based on the complex (BSA-phenylfluorone (PF)) adsorbed on beta-CDCP. Adsorption kinetics and various factors of the formation procedure of BSA-PF and its retention on beta-CDCP were investigated. The linear range and detection limit (DL) was 20.0-200.0 and 0.03 mg/L, respectively. Moreover. the beta-CDCP could be used repeatedly. The proposed method has been applied to analysis serum albumin with satisfactory results. (c) 2010 Elsevier B.V. All rights reserved.

: [Wu, Min; Zhu, Xiashi] Yangzhou Univ, Dept Chem, Yangzhou 225002, Peoples R China

: Zhu, XS, Yangzhou Univ, Dept Chem, Yangzhou 225002, Peoples R China.

: [email protected]

:


20875082

Foundation of the Educational Committee of Jiangsu Provincial Universities Excellence Science and Technology Invention Team in Yangzhou University

The authors acknowledge the financial support from the National Natural Science Foundation of China (20875082) and the Foundation of the Educational Committee of Jiangsu Provincial Universities Excellence Science and Technology Invention Team in Yangzhou University.

: 13

: 0

: PERGAMON-ELSEVIER SCIENCE LTD

: THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND

ISSN: 1386-1425

DOI: 10.1016/j.saa.2010.08.061

29 : SPECTROCHIM ACTA PT A-MOL BIO

ISO : Spectroc. Acta Pt. A-Molec. Biomolec. Spectr.

: 4

: Spectroscopy

ISI : 690AF

5 415

: Qiang, CK (Qiang Cheng-kui); Du, YZ (Du Yu-zhou); Yu, LY (Yu Lin-ya); Cui, YD (Cui Ya-dong); Zhou, BY (Zhou Bao-ya)

: Cloning and Expression of Heat Shock Protein 90 Gene from the Diapausing Larvae of the Rice Stem Borer, Chilo Suppressalis (Lepidoptera: Pyralidae) exposed to Temperature Stress

: RESEARCH JOURNAL OF BIOTECHNOLOGY, 5 (4): 68-75 NOV 2010

: English

: Article

: Chilo suppressalis Walker; heat shock protein 90; temperature stress; expression level

KeyWords Plus: MOLECULAR CHAPERONES

: The rice stem borer, Chilo suppressalis Walker, is an economically important pest distributed in rice producing regions of China To understand its molecular adaptation, the authors cloned the cDNA sequence of CcsHsp90 which is essential for dealing with many environmental stresses in all organisms and measured the mRNA expression in the diapausing larvae exposed to temperature stress The results showed that CcsHsp90 (GenBank accession no FJ866608) is potentially encoded to 312 amino acids with calculated molecular weight of 35 59KDa and the theoretical isoeletric point of 4 72 The deduced amino acid sequence of CcsHsp90 is displayed over 80% of the degree of conservation to other Lepidopteran insects The relative mRNA expression levels of CcsHsp90 in the diapausing larvae which were exposed to different temperatures from 28 degrees C (CK) to -14 degrees C with a gradient of 7 degrees C for 4h increased first and decreased later and peaked at 0 C Then the larvae were exposed to 0 degrees C for 0 similar to 8h, the change of the levels was the same as the above and peaked at 4h treatment Therefore, it could be concluded that 0 degrees C for 4h treatment might induce the maximal expression of CcsHsp90 in the diapausing larvae of the rice stem borer

: [Qiang Cheng-kui; Du Yu-zhou; Yu Lin-ya] Yangzhou Univ, Inst Appl Entomol, Yangzhou 225009, Peoples R China; [Qiang Cheng-kui; Zhou Bao-ya] Xuzhou Bioengn Tech Coll, Dept Agr & Landscape Engn, Xuzhou 221006, Peoples R China; [Cui Ya-dong] Fuyang Teachers Coll, Coll Life Sci, Fuyang 236032, Peoples R China

: Qiang, CK, Yangzhou Univ, Inst Appl Entomol, Yangzhou 225009, Peoples R China.

:

National Basic Research Program (973 Program)

2006CB102002

The authors thank several anonymous reviewers for insightful comments that greatly improved the manuscript and all the laboratory members for technical advice and helpful discussions This work was supported by the National Basic Research Program (973 Program) (No 2006CB102002)

: 14

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: RESEARCH JOURNAL BIOTECHNOLOGY

: SECTOR A-80, SCHEME NO 54, VIJAY NAGAR, A B ROAD, INDORE, 452 010 MP, INDIA

ISSN: 0973-6263

29 : RES J BIOTECHNOL

ISO : Res. J. Biotechnol.

: 8

: Biotechnology & Applied Microbiology

ISI : 690ET

6 415

: Chen, YT (Chen Ying-Tian); Ho, TH (Ho Tso-Hsiu); Lim, CS (Lim Chern-Sing); Lim, BH (Lim Boon Han)

: Development of silicon purification by strong radiation catalysis method

: CHINESE PHYSICS B, 19 (11): Art. No. 118105 NOV 2010

: English

: Article

: silicon purification; strong radiation catalysis; boron removal; solar furnace

KeyWords Plus: NONIMAGING FOCUSING HELIOSTAT; PROTOTYPE

: Using a new type of solar furnace and a specially designed induction furnace cost effective and highly efficient purification of metallurgical silicon into solar grade silicon can be achieved It is realized by a new method for extracting boron from silicon with the aid of photo chemical effect In this article, we discussed the postulated principle of strong radiation catalysis and the recent development in practice Starting from ordinary metallurgical silicon, we achieved a purification result of 0 12 ppmw to 0 3 ppmw of boron impurity in silicon by only single pass of a low cost and simple process, the major obstacle to make 'cheap' solar grade silicon feedstock in industry is thus removed

: [Chen Ying-Tian] So Univ Sci & Technol, Shenzhen 518055, Peoples R China; [Chen Ying-Tian; Ho Tso-Hsiu] Chinese Acad Sci, Inst Theoret Phys, Beijing 100190, Peoples R China; [Chen Ying-Tian; Lim Chern-Sing; Lim Boon Han] Yangzhou Univ, Yangzhou 225009, Peoples R China

: Chen, YT, So Univ Sci & Technol, Shenzhen 518055, Peoples R China.

: 8

: 0

: IOP PUBLISHING LTD

: DIRAC HOUSE, TEMPLE BACK, BRISTOL BS1 6BE, ENGLAND

ISSN: 1674-1056

: 118105

29 : CHIN PHYS B

ISO : Chin. Phys. B

: 7

: Physics, Multidisciplinary

ISI : 690AS

7 415

: Yang, PH (Yang, Penghui); Tang, C (Tang, Chong); Luo, DY (Luo, Deyan); Zhan, ZP (Zhan, Zhongpeng); Xing, L (Xing, Li); Duan, YQ (Duan, Yueqiang); Jia, WH (Jia, Weihong); Peng, DX (Peng, Daxin); Liu, XF (Liu, Xiufan); Wang, XL (Wang, Xiliang)

: Cross-clade protection against HPAI H5N1 influenza virus challenge in BALB/c mice intranasally administered adjuvant-combined influenza vaccine

: VETERINARY MICROBIOLOGY, 146 (1-2): 17-23 NOV 20 2010

: English

: Article

: H5N1 influenza vaccine; Mucosal immunity; Adjuvant

KeyWords Plus: MF59-ADJUVANTED VACCINE; IMMUNIZATION; IMMUNITY; MUCOSAL; TRIAL; HEMAGGLUTININ; COMBINATION; INFECTION; RESPONSES; ANTIBODY

: The avian H5N1 influenza virus has the potential to cause a new pandemic. The increasing number of recent outbreaks of highly pathogenic avian influenza H5N1 in birds and humans emphasizes the urgent need to develop a potent H5N1 vaccine. Here, we studied the immunogenicity and protective effect of a vaccine prepared from H5N1 inactivated whole virus. This vaccine was intranasally co-administered in mice with phosphate buffered saline, recombinant cholera toxin B subunit (rCTB), cholera toxin (CT), rCTB containing a trace amount of holotoxin (rCTB/CT), polyinosinic:polycytidylic acid double-stranded RNA (polyI:C), or MF59 as an adjuvant. Intranasal administration of H5N1 inactivated whole virus vaccine with rCTB, CT, rCTB/CT, polyI:C, and MF59 elicited an immunological response with both secretory IgA (sIgA) in nasal, lung, and vaginal lavage, and IgG antibody in serum, showing protective immunity against lethal H5N1 infection. Cross-clade protection was also observed in animals immunized with a vaccine derived from Anhui/01/2005(H5N1) with rCTB, CT, rCTB/CT, polyI:C, or MF59 as adjuvants that were subsequently challenged with the A/OT/SZ/097/03 influenza strain. (C) 2010 Elsevier B.V. All rights reserved.

: [Yang, Penghui; Tang, Chong; Luo, Deyan; Zhan, Zhongpeng; Xing, Li; Duan, Yueqiang; Jia, Weihong; Wang, Xiliang] Beijing Inst Microbiol & Epidemiol, State Key Lab Pathogen & Biosecur, Beijing 100071, Peoples R China; [Peng, Daxin; Liu, Xiufan] Yangzhou Univ, Minist Agr, Key Lab Anim Infect Dis, Yangzhou 225009, Peoples R China

: Wang, XL, Beijing Inst Microbiol & Epidemiol, State Key Lab Pathogen & Biosecur, 20 Dong Da St, Beijing 100071, Peoples R China.

: [email protected]

:

Chinese National Scientific and Technical Supporting Program

2009CB522102

Chinese National Programs for High Technology Research and Development

2006AA02Z450

We are grateful to Prof. Qingjun Ma for providing us with the rCTB. This study was partially funded by the Chinese National Scientific and Technical Supporting Program (2009CB522102), Chinese National Programs for High Technology Research and Development, under contract no. 2006AA02Z450. No other potential conflict of interest relevant to this article is reported.

: 28

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: ELSEVIER SCIENCE BV

: PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS

ISSN: 0378-1135

DOI: 10.1016/j.vetmic.2010.03.024

29 : VET MICROBIOL

ISO : Vet. Microbiol.

: 7

: Microbiology; Veterinary Sciences

ISI : 685YU

8 415

: Sun, J (Sun, Jing); Wu, Q (Wu, Qun); Xia, EY (Xia, Er-Yan); Yan, CG (Yan, Chao-Guo)

: Diastereoselective Synthesis of trans-2,3-Dihydrothiophenes via Multicomponent Reactions of Pivaloylacetonitrile, Aldehyde, Amine, and Thiazolidinedione

: SYNTHESIS-STUTTGART, (23): 3987-3992 DEC 2010

: English

: Article

: multicomponent reaction; thiazolidinedione; pivaloylacetonitrile; dihydrothiophene

KeyWords Plus: ONE-POT SYNTHESIS; ONE-STEP SYNTHESIS; DOMINO REACTIONS; GEWALD REACTION; REACTIONS AMCRS; AQUEOUS-MEDIA; MALONONITRILE; DERIVATIVES; CHEMISTRY; AGENTS

: Polyfunctionalized trans-2,3-dihydrothiophenes are efficiently synthesized from the four-component reactions of thiazolidine-2,4-dione, aromatic aldehydes, secondary amines, and pivaloylacetonitrile. The reaction mechanism involves Knoevenagel condensation, Michael addition, and characteristic ring-opening of thiazolidine-2,4-dione followed by an intramolecular ring-closure process

: [Sun, Jing; Wu, Qun; Xia, Er-Yan; Yan, Chao-Guo] Yangzhou Univ, Coll Chem & Chem Engn, Yangzhou 225002, Peoples R China

: Yan, CG, Yangzhou Univ, Coll Chem & Chem Engn, Yangzhou 225002, Peoples R China.

: [email protected]

:


20972132

This work was financially supported by the National Natural Science Foundation of China (Grant No. 20972132).

: 41

: 0

: GEORG THIEME VERLAG KG

: RUDIGERSTR 14, D-70469 STUTTGART, GERMANY

ISSN: 0039-7881

DOI: 10.1055/s-0030-1258272

29 : SYNTHESIS-STUTTGART

ISO : Synthesis

: 6

: Chemistry, Organic

ISI : 684FN

9 415

: Han, Y (Han, Ying); Hou, H (Hou, Hong); Yao, R (Yao, Rong); Fu, Q (Fu, Qin); Yan, CG (Yan, Chao-Guo)

: Diastereoselective Synthesis of trans-2,3,6,7-Tetrahydro-4(5H)-benzofuranones and trans-2,3-Dihydrofurocoumarins via Pyridinium Ylide Assisted Tandem Reactions

: SYNTHESIS-STUTTGART, (23): 4061-4067 DEC 2010

: English

: Article

: tandem reaction; pyridinium ylide; benzofuranone; furocoumarin; diastereoselective

KeyWords Plus: ONE-STEP SYNTHESIS; ORGANIC-SYNTHESIS; MULTICOMPONENT REACTIONS; ACRIDONE ALKALOIDS; MICHAEL ADDITION; CYCLIZATION; MALONONITRILE; NITROOLEFINS; QUINAZOLINE; DERIVATIVES

: An efficient, one-pot tandem reaction of pyridine, an alpha-haloacetate, an aromatic aldehyde and cyclohexane-1,3-dione, dimedone or 4-hydrocoumarin in acetonitrile using 1,4-diazabicyclo[2.2.2]octane as a basic catalyst is described. The products, 2,3,6,7-tetrahydro-4(5H)-benzofuranones and 2,3-dihydrofurocoumarins, are obtained in a diastereoselective manner via a mechanism involving in situ formation of a pyridinium ylide. H-1 NMR spectroscopy and single crystal X-ray analysis indicates that the products are formed exclusively as the trans isomers.

: [Han, Ying; Hou, Hong; Yao, Rong; Fu, Qin; Yan, Chao-Guo] Yangzhou Univ, Coll Chem & Chem Engn, Yangzhou 225002, Peoples R China

: Yan, CG, Yangzhou Univ, Coll Chem & Chem Engn, Yangzhou 225002, Peoples R China.

: [email protected]

:


20972132

This work was supported financially by the National Natural Science Foundation of China (Grant No. 20972132).

: 33

: 0

: GEORG THIEME VERLAG KG

: RUDIGERSTR 14, D-70469 STUTTGART, GERMANY

ISSN: 0039-7881

DOI: 10.1055/s-0030-1258279

29 : SYNTHESIS-STUTTGART

ISO : Synthesis

: 7


ISI : 684FN

10 415

: Wu, CL (Wu, Changle); Shen, L (Shen, Li); Huang, QL (Huang, Qingli); Zhang, YC (Zhang, Yong-Cai)

: Synthesis of Na-doped ZnO nanowires and their antibacterial properties

: POWDER TECHNOLOGY, 205 (1-3): 137-142 JAN 10 2011

: English

: Article

: ZnO; Chemical synthesis; Antibacterial properties

KeyWords Plus: ZINC-OXIDE NANOWIRES; P-TYPE ZNO; OPTICAL-PROPERTIES; THIN-FILMS; NANOSTRUCTURES; LUMINESCENT; NANOCRYSTALS; DEPOSITION; DEFECTS; SENSOR

: Na-doped ZnO nanowires with an average diameter of similar to 40 nm have been fabricated by a thermal decomposition route at temperatures around 400 degrees C. Their properties have been investigated using X-ray diffraction, field emission scanning electron microscope, Raman spectra, photoluminescence spectra and Xray photoelectron spectra. Photoluminescence spectra showed that the as-synthesized ZnO samples exhibited strong visible emission with a major peak at 420 nm. Furthermore, intensity of the visible emission at 420 nm enhanced as the increase of Na concentration. The improvement of visible emission in the Na-doped ZnO samples should be a result of the surface defects increased by doping of Na in ZnO. Their antibacterial activities were also evaluated by determination of minimum inhibitory concentration (MIC) against Escherichia coli 25922 in vitro. Antibacterial tests indicated these nanomaterials showed good antibacterial properties after UV illumination for a short time. (C) 2010 Elsevier B.V. All rights reserved.

: [Wu, Changle; Huang, Qingli] Yangzhou Univ, Testing Ctr, Yangzhou 225009, Jiangsu, Peoples R China; [Shen, Li; Zhang, Yong-Cai] Yangzhou Univ, Coll Chem & Chem Engn, Yangzhou 225002, Peoples R China

: Wu, CL, Yangzhou Univ, Testing Ctr, Yangzhou 225009, Jiangsu, Peoples R China.

: [email protected]

:

University Science And Technology Innovation Foundation of Yangzhou University

2009CXJ055

This work is supported by the University Science And Technology Innovation Foundation of Yangzhou University (No. 2009CXJ055).

: 41

: 0

: ELSEVIER SCIENCE SA

: PO BOX 564, 1001 LAUSANNE, SWITZERLAND

ISSN: 0032-5910

DOI: 10.1016/j.powtec.2010.09.003

29 : POWDER TECHNOL

ISO : Powder Technol.

: 6

: Engineering, Chemical

ISI : 687ON

11 415

: Tang, XA (Tang, Xiang); Zhang, ZY (Zhang, Zhi-Yong); Zhang, WJ (Zhang, Wen-Juan); Zhao, XM (Zhao, Xing-Ming); Li, XA (Li, Xuan); Zhang, D (Zhang, Dong); Liu, QQ (Liu, Qiao-Quan); Tang, WH (Tang, Wei-Hua)

: Global Gene Profiling of Laser-Captured Pollen Mother Cells Indicates Molecular Pathways and Gene Subfamilies Involved in Rice Meiosis

: PLANT PHYSIOLOGY, 154 (4): 1855-1870 DEC 2010

: English

: Article

KeyWords Plus: FUNCTIONAL DIVERSIFICATION; HOMOLOGOUS RECOMBINATION; STAMEN DEVELOPMENT; MALE GAMETOPHYTE; DNA-REPLICATION; FLORAL ORGAN; BOX GENES; F-BOX; EXPRESSION; PROTEIN

: Pollen mother cells (PMCs) represent a critical early stage in plant sexual reproduction in which the stage is set for male gamete formation. Understanding the global molecular genetics of this early meiotic stage has so far been limited to whole stamen or floret transcriptome studies, but since PMCs are a discrete population of cells in developmental synchrony, they provide the potential for precise transcriptome analysis and for enhancing our understanding of the transition to meiosis. As a step toward identifying the premeiotic transcriptome, we performed microarray analysis on a homogenous population of rice (Oryza sativa) PMCs isolated by laser microdissection and compared them with those of tricellular pollen and seedling. Known meiotic genes, including OsSPO11-1, PAIR1, PAIR2, PAIR3, OsDMC1, OsMEL1, OsRAD21-4, OsSDS, and ZEP1, all showed preferential expression in PMCs. The Kyoto Encyclopedia of Genes and Genomes pathways significantly enriched in PMC-preferential genes are DNA replication and repair pathways. Our genome-wide survey showed that, in the buildup to meiosis, PMCs accumulate the molecular machinery for meiosis at the mRNA level. We identified 1,158 PMC-preferential genes and suggested candidate genes and pathways involved in meiotic recombination and meiotic cell cycle control. Regarding the developmental context for meiosis, the DEF-like, AGL2-like, and AGL6-like subclades of MADS box transcription factors are PMC-preferentially expressed, the trans-zeatin type of cytokinin might be preferentially synthesized, and the gibberellin signaling pathway is likely active in PMCs. The ubiquitin-mediated proteolysis pathway is enriched in the 127 genes that are expressed in PMCs but not in tricellular pollen or seedling.

: [Tang, Xiang; Zhang, Zhi-Yong; Zhang, Dong; Tang, Wei-Hua] Chinese Acad Sci, Natl Key Lab Plant Mol Genet, Inst Plant Physiol & Ecol, Shanghai Inst Biol Sci, Shanghai 200032, Peoples R China; [Li, Xuan] Chinese Acad Sci, Key Lab Synthet Biol, Inst Plant Physiol & Ecol, Shanghai Inst Biol Sci, Shanghai 200032, Peoples R China; [Tang, Xiang; Liu, Qiao-Quan] Yangzhou Univ, Minist Educ, Key Lab Plant Funct Genom, Yangzhou 225009, Peoples R China; [Zhang, Wen-Juan; Zhao, Xing-Ming] Shanghai Univ, Inst Syst Biol, Shanghai 200444, Peoples R China

: Tang, WH, Chinese Acad Sci, Natl Key Lab Plant Mol Genet, Inst Plant Physiol & Ecol, Shanghai Inst Biol Sci, Shanghai 200032, Peoples R China.

: [email protected]

:

Ministry of Science and Technology of China

2007CB108700

2011CB944600

2006AA10A102

2009ZX08009-136B


30770196

Shanghai Rising Star Program

10QA1402700

This work was supported by the Ministry of Science and Technology of China (grant nos. 2007CB108700, 2011CB944600, 2006AA10A102, and 2009ZX08009-136B), the National Natural Science Foundation of China (grant no. 30770196), and the Shanghai Rising Star Program (grant no. 10QA1402700 to X.-M.Z.).

: 69

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: AMER SOC PLANT BIOLOGISTS

: 15501 MONONA DRIVE, ROCKVILLE, MD 20855 USA

ISSN: 0032-0889

DOI: 10.1104/pp.110.161661

29 : PLANT PHYSIOL

ISO : Plant Physiol.

: 16

: Plant Sciences

ISI : 688FA

12 415

: Feng, LG (Feng, Li-Guo); Chen, C (Chen, Chen); Sheng, LX (Sheng, Li-Xia); Liu, P (Liu, Ping); Tao, J (Tao, Jun); Su, JL (Su, Jia-Le); Zhao, LY (Zhao, Lan-Yong)

: Comparative Analysis of Headspace Volatiles of Chinese Rosa rugosa

: MOLECULES, 15 (11): 8390-8399 NOV 2010

: English

: Article

: Rosa rugosa; headspace volatiles; content; gas chromatography-mass spectrometry

: The floral headspace compounds of Chinese Rosa rugosa germplasms that were isolated by an automated headspace sampler with built-in trap, and followed by gas chromatography-mass spectrometry for identification and quantification. Up to 33 volatile compounds were identified from the 23 rose germplasms, including nine alcohols, five esters, three alkanes, 10 terpenes, three aldehydes, two ketones, and one ether. The main floral components identified were 2-phenylethanol, beta-citronellol, ethanol, and n-hexane. 'xizi', 'miaofengshan', 'xiangciguo', and 'tangbai' contained the highest amounts of 2-phenylethanol at 84.66 mu g.g(-1), beta-citronellol at 70.98 mu g.g(-1), ethanol at 83.87 mu g.g(-1), and n-hexane at 18.23 mu g.g(-1), respectively. 'Rongchengyesheng', 'tanghong', 'xizi', 'miaofengshan', and 'baizizhi' could be considered good materials for extracting rose oil and breeding new cultivars.

: [Liu, Ping; Zhao, Lan-Yong] Shandong Agr Univ, Coll Forestry, Tai An 271018, Shandong, Peoples R China; [Feng, Li-Guo; Chen, Chen; Sheng, Li-Xia; Tao, Jun] Yangzhou Univ, Coll Hort & Plant Protect, Yangzhou 225009, Jiangsu, Peoples R China; [Su, Jia-Le] Jiangsu Acad Agr Sci, Inst Hort, Nanjing 210014, Jiangsu, Peoples R China

: Zhao, LY, Shandong Agr Univ, Coll Forestry, Tai An 271018, Shandong, Peoples R China.

: [email protected]; [email protected]; [email protected]; [email protected]; [email protected]; [email protected]

:


31000319

Jiangsu Provincial Natural Science Foundation of China

BK2009189

Jiangsu Postdoctoral Sustentation Fund of China

0902057C

This research was funded by National Natural Science Foundation of China (Grant No. 31000319), Jiangsu Provincial Natural Science Foundation of China (Grant No. BK2009189), and Jiangsu Postdoctoral Sustentation Fund of China (Grant No. 0902057C).

: 0

: MDPI AG

: KANDERERSTRASSE 25, CH-4057 BASEL, SWITZERLAND

ISSN: 1420-3049

DOI: 10.3390/molecules15118390

29 : MOLECULES

ISO : Molecules

: 10


ISI : 684VR

13 415

: Chen, M (Chen, Ming); Shang, T (Shang, Ting); Liu, J (Liu, Jie); Diao, GW (Diao, Guowang)

: Complexation thermodynamics between butyl rhodamine B and calix[n]arenesulfonates (n=4, 6, 8)

: JOURNAL OF CHEMICAL THERMODYNAMICS, 43 (1): 88-93 JAN 2011

: English

: Article

: Inclusion complex; Calix[n]arenesulfonates (n=4 6 8); Butyl rhodamine B; Stability constant; Thermodynamics parameter; Fluorescence spectroscopy

KeyWords Plus: CYCLODEXTRINS; MOLECULES

: The characteristics of host-guest complexation between water-soluble calix[n]arenesulfonates (CnS n = 4 6 8) and butyl rhodamine B (BRB) were investigated by fluorescence spectrometry Fluorescence spectroscopy experiments were performed in pH 8 0 Tris(3-aminomethane)-HCl buffer solution at different temperature to calculate the stability constants (K-S) for the stoichiometric 1 1 inclusion complexes of C4S C6S and C8S with BRB The complex stability constant monotonically Increased with the number of phenolic units in the calixarene ring The thermodynamic parameters at T = 298 K for the inclusion complexes were calculated through Van t Hoff analysis The inclusion complexes of CnS with BRB were driven by the favorable enthalpic changes accompanying negative entropy changes The stability constants were affected by the acidity of the solution When pH was 8 0 the stability constants reached the maximum The complex Interaction was mainly attributed to the weak forces including electrostatic interaction and hydrogen bonding (C) 2010 Elsevier Ltd All rights reserved

: [Diao, Guowang] Yangzhou Univ, Coll Chem & Chem Engn, Yangzhou 225002, Peoples R China; Key Lab Environm Mat Environm Engn Jiangsu Prov, Yangzhou 225002, Jiangsu, Peoples R China

: Diao, GW, Yangzhou Univ, Coll Chem & Chem Engn, Yangzhou 225002, Peoples R China.

:


20773107

20973151

20901065

Natural Science Key Foundation of Educational Committee of Jiangsu Province of China

07KJA15015

Foundation of Jiangsu Provincial in Yangzhou University

Foundation of the Educational Committee of Jiangsu Provincial Universities Excellence Science and Technology Invention Team in Yangzhou University

The authors acknowledge the financial support from the National Natural Science Foundation of China (Grant Nos 20773107 20973151 20901065) the Natural Science Key Foundation of Educational Committee of Jiangsu Province of China (Grant No 07KJA15015) the Foundation of Jiangsu Provincial Key Program of Physical Chemistry in Yangzhou University and the Foundation of the Educational Committee of Jiangsu Provincial Universities Excellence Science and Technology Invention Team in Yangzhou University

: 19

: 0

: ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD

: 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND

ISSN: 0021-9614

DOI: 10.1016/j.jct.2010.08.014

29 : J CHEM THERMODYN

ISO : J. Chem. Thermodyn.

: 6

: Thermodynamics; Chemistry, Physical

ISI : 681HS

14 415

: Wei, CX (Wei, Cunxu); Qin, FL (Qin, Fengling); Zhou, WD (Zhou, Weidong); Yu, HG (Yu, Huaguang); Xu, B (Xu, Bin); Chen, C (Chen, Chong); Zhu, LJ (Zhu, Lijia); Wang, YP (Wang, Youping); Gu, MH (Gu, Minghong); Liu, QQ (Liu, Qiaquan)

: Granule Structure and Distribution of Allomorphs in C-Type High-Amylose Rice Starch Granule Modified by Antisense RNA Inhibition of Starch Branching Enzyme

: JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 58 (22): 11946-11954 NOV 24 2010

: English

: Article

: Rice (Oryza sativa L.); high-amylose starch granule; C-type starch; allomorph distribution; acid hydrolysis

KeyWords Plus: C-13 CP/MAS NMR; SOLID-STATE NMR; ACID-HYDROLYSIS; PHYSICOCHEMICAL PROPERTIES; MAIZE STARCHES; PEA STARCH; ULTRASTRUCTURE; WATER; ORGANIZATION; CORN

: C-type starch, which is a combination of both A-type and B-type crystal starch, is usually found in legumes and rhizomes. We have developed a high-amylose transgenic line of rice (TRS) by antisense RNA inhibition of starch branching enzymes. The starch in the endosperm of this TRS was identified as typical C-type crystalline starch, but its fine granular structure and allomorph distribution remained unclear. In this study, we conducted morphological and spectroscopic studies on this TRS starch during acid hydrolysis to determine the distribution of A- and B-type allomorphs. The morphology of starch granules after various durations of acid hydrolysis was compared by optical microscopy, scanning electron microscopy, and transmission electron microscopy. The results showed that amorphous regions were located at the center part of TRS starch subgranules. During acid hydrolysis, starch was degraded from the interior of the subgranule to the outer surface, while the peripheral part of the subgranules and the surrounding band of the starch granule were highly resistant to acid hydrolysis. The spectroscopic changes detected by X-ray powder diffraction, C-13 cross-polarization magic-angle spinning NMR, and attenuated total reflectance Fourier transform infrared showed that the A-type allomorph was hydrolyzed more rapidly than the B-type, and that the X-ray diffraction profile gradually changed from a native C-type to a CB-type with increasing hydrolysis time. Our results showed that, in TRS starch, the A-type allomorph was located around the amorphous region, and was surrounded by the B-type allomorph located in the peripheral region of the subgranules and the surrounding band of the starch granule. Thus, the positions of A- and B-type allomorphs in the TRS C-type starch granule differ markedly from those in C-type legume and rhizome starch.

: [Wei, Cunxu; Zhu, Lijia; Gu, Minghong; Liu, Qiaquan] Yangzhou Univ, Key Labs Crop Genet & Physiol Jiangsu Prov & Plan, Minist Educ, Yangzhou 225009, Peoples R China; [Wei, Cunxu; Qin, Fengling; Wang, Youping] Yangzhou Univ, Coll Biosci & Biotechnol, Yangzhou 225009, Peoples R China; [Zhou, Weidong; Yu, Huaguang; Xu, Bin; Chen, Chong] Yangzhou Univ, Testing Ctr, Yangzhou 225009, Peoples R China

: Liu, QQ, Yangzhou Univ, Key Labs Crop Genet & Physiol Jiangsu Prov & Plan, Minist Educ, Yangzhou 225009, Peoples R China.

: [email protected]; [email protected]

:


30828021

30971754

31071342

Ministry of Science and Technology

2009ZX08011-003B

2008ZX08009-003

Government of Jiangsu Province

BK2009186

China Postdoctoral Science Foundation

20090451252

Received for review June 24, 2010. Revised manuscript received October 12, 2010. Accepted October 13, 2010. This study was financially supported by grants from the National Natural Science Foundation of China (30828021, 30971754, 31071342), the Ministry of Science and Technology (2009ZX08011-003B, 2008ZX08009-003), the Government of Jiangsu Province (BK2009186), and the China Postdoctoral Science Foundation (20090451252).

: 33

: 0

: AMER CHEMICAL SOC

: 1155 16TH ST, NW, WASHINGTON, DC 20036 USA

ISSN: 0021-8561

DOI: 10.1021/jf103412d

29 : J AGR FOOD CHEM

ISO : J. Agric. Food Chem.

: 9

: Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology

ISI : 680AR

15 415

: Xu, PH (Xu, Peihu); Guo, FF (Guo, Fengfeng); Huang, J (Huang, Jin); Zhou, SF (Zhou, Shaofeng); Wang, DX (Wang, Daxin); Yu, JH (Yu, Jiahui); Chen, JH (Chen, Jinghua)

: Alginate-based ferrofluid and magnetic microsphere thereof

: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 47 (5): 654-660 DEC 1 2010

: English

: Article

: Alginate; Ferrofluids; Magnetic microspheres; Drug release

KeyWords Plus: IRON-OXIDE NANOPARTICLES; COATED SUPERPARAMAGNETIC NANOPARTICLES; BIOMEDICAL APPLICATIONS; MEDICAL APPLICATION; CANCER-TREATMENT; CONTRAST AGENTS; DELIVERY; IMMOBILIZATION; MATRIX; FLUID

: The Fe3O4 ferrofluids have been prepared using sodium alginate (Na-AL) as a stabilizing agent The alginate can prevent the aggregation of magnetic nanoparticles and hence contributed to higher stability for the ferrofluids Furthermore the alginate component in the ferrofluids was crosslinked by Ca2+ to produce magnetic microspheres The swelling behavior of magnetic microspheres showed a pH-dependence and hence determined the drug release process under various pH conditions The presence of the Fe3O4 nanoparticles made the magnetic microspheres swell more easily Meanwhile the strong ability to absorb the drug for the incorporated Fe3O4 nanoparticles decreased the release rate and hence was more favorable to the sustaining release of drug Except for the controlled delivery and release of drug the alginate-based ferrofluids and magnetic microspheres in this work might also show a great potential for other biomedical and biotechnological applications such as magnetic targeting magnetic separation and magnetic resonance imaging (C) 2010 Elsevier B V All rights reserved

: [Xu, Peihu; Guo, Fengfeng; Huang, Jin; Zhou, Shaofeng] Wuhan Univ Technol, Coll Chem Engn, Wuhan 430070, Peoples R China; [Huang, Jin] S China Univ Technol, State Key Lab Pulp & Paper Engn, Guangzhou 510640, Guangdong, Peoples R China; [Huang, Jin; Yu, Jiahui] E China Normal Univ, Inst Adv Interdisciplinary Res, Shanghai 200062, Peoples R China; [Wang, Daxin] Yangzhou Univ, Subei Hosp Jiangsu Prov, Yangzhou 225001, Peoples R China; [Chen, Jinghua] Jiangnan Univ, Sch Med & Pharmaceut, Wuxi 214122, Peoples R China

: Huang, J, Wuhan Univ Technol, Coll Chem Engn, Wuhan 430070, Peoples R China.

:


50873080

50843031

973 Projects of Chinese Ministry of Science and Technology

2007CB936104

2009CB930300

Shanghai Municipality Commission for Special Project of Nanometer Science and Technology

0952nm05300

Shanghai Municipality Commission for International Corporation Project

10410710000

Non-Governmental International Corporation Project

09540709000

Foundation for New Century Excellent Talents in University of Ministry of Education of China

NCET-10-0435

State Key Laboratory of Pulp and Paper Engineering

200906

Fundamental Research Funds for the Central Universities (Self-Determined and Innovative Research Funds of WUT)

2010-11-022

JUSRT30905

The research work was financially supported by the National Natural Science Foundation of China (50873080 and 50843031) 973 Projects of Chinese Ministry of Science and Technology (2007CB936104 and 2009CB930300) Shanghai Municipality Commission for Special Project of Nanometer Science and Technology (0952nm05300) Shanghai Municipality Commission for International Corporation Project (10410710000) and for Non-Governmental International Corporation Project (09540709000) Foundation for New Century Excellent Talents in University of Ministry of Education of China (NCET-10-0435) State Key Laboratory of Pulp and Paper Engineering (200906) and Fundamental Research Funds for the Central Universities (Self-Determined and Innovative Research Funds of WUT 2010-11-022 and JUSRT30905)

: 27

: 0



ISSN: 0141-8130

DOI: 10.1016/j.ijbiomac.2010.08.012

29 : INT J BIOL MACROMOL

ISO : Int. J. Biol. Macromol.

: 7

: Biochemistry & Molecular Biology

ISI : 685VA

16 415

: Yang, GJ (Yang, Gongjun); Yan, JK (Yan, Jiankang); Qi, F (Qi, Fen); Sun, C (Sun, Cheng)

: High Sensitivity and Reproducibility of a Bismuth/Poly(bromocresol purple) Film Modified Glassy Carbon Electrode for Determination of Trace Amount of Cadmium by Differential Pulse Anodic Stripping Voltammetry

: ELECTROANALYSIS, 22 (22): 2729-2738 NOV 2010

: English

: Article

: Bismuth/poly(bromocresol purple) film modified electrode; Cadmium; Differential pulse anodic stripping voltammetry

KeyWords Plus: WATER SAMPLES; HEAVY-METALS; IONS; CD(II); PB(II); LEAD; VOLTARNMETRY; NETWORK; ZN; PB

: This work described a novel type of bismuth/poly(bromocresol purple) film modified glassy carbon electrode (denoted as Bi/Poly(BCP)/GCE) for anodic stripping analysis of trace Cd2+. The Bi/Poly(BCP)/GCE was fabricated in situ by depositing simultaneously bismuth and cadmium by reduction at -1.20 V on the poly(BCP) film using a differential pulse voltammetry. Under the optimum conditions, the anodic stripping peak current response increased linearly with the Cd2+ concentrations in a range of 2.0 x 10(-8)-1.0 x1.0(-7) M and 1.0 x 10(-7)-6.0x10(-6) M in 0.1 M NaAc-Ac buffer solution (pH 5.0) with the detection limit of 6.5 x 10(-9) M (S/N =3). The Bi/poly(BCP)/GCE performed good reproducibility and high sensitivity. Finally, this proposed method was successfully applied to determine the concentration of Cd2+ in water samples.

: [Yang, Gongjun] China Pharmaceut Univ, Sch Pharm, Nanjing 210009, Peoples R China; [Yang, Gongjun; Yan, Jiankang; Qi, Fen] Yangzhou Univ, Coll Chem & Chem Engn, Yangzhou 225002, Peoples R China; [Sun, Cheng] Nanjing Univ, Sch Environm, State Key Lab Pollut Control & Resource Reuse, Nanjing 210093, Peoples R China

: Yang, GJ, China Pharmaceut Univ, Sch Pharm, Nanjing 210009, Peoples R China.


:

Fund of State Key Laboratory of Pollution Control and Resource Reuse

PCRRF08012

This project was financially supported by the Fund of State Key Laboratory of Pollution Control and Resource Reuse (PCRRF08012).

: 38

: 0

: WILEY-V C H VERLAG GMBH

: PO BOX 10 11 61, D-69451 WEINHEIM, GERMANY

ISSN: 1040-0397

DOI: 10.1002/elan.201000260

29 : ELECTROANAL

ISO : Electroanalysis

: 10

: Chemistry, Analytical; Electrochemistry

ISI : 689GF

17 415

: Liu, J (Liu, Jing); Jiang, ZZ (Jiang, Zhenzhou); Liu, L (Liu, Li); Zhang, Y (Zhang, Yun); Zhang, SA (Zhang, Shuang); Xiao, JW (Xiao, Jingwei); Ma, M (Ma, Min); Zhang, LY (Zhang, Luyong)

: Triptolide induces adverse effect on reproductive parameters of female Sprague-Dawley rats

: DRUG AND CHEMICAL TOXICOLOGY, 34 (1): 1-7 JAN 2011

: English

: Article

: Triptolide; reproductive parameters; ER alpha; hormones; estrous cycle; female rats

KeyWords Plus: TRIPTERYGIUM-WILFORDII; ESTROUS-CYCLE; PROGESTERONE-RECEPTOR; INHIBITION; ARTHRITIS; EXTRACT

: The aim of this study was to investigate the adverse effects of triptolide, a diterpenoid triepoxid and a major active component isolated from Triptergium wilfordii Hook.f (TWHF), on various reproductive parameters of female Sprague-Dawley (SD) rats. Female SD rats were treated with triptolide by oral administration (gastric infusion; 0, 100, 200, and 400 mu g/kg) once-daily for 90 days. During the experimental period, vaginal smears were taken to monitor the estrous phase for the last 30 days. Relative ovary and uterus weights to body and histopathologically changes were determined on the last day. Serum levels of estradiol (E-2), progesterone (P), follicle-stimulating hormone (FSH), and luteotropic hormone (LH) were assessed by enzyme immunoassay. ER alpha expression in the uterus and ovaries were analyzed from using immunohistochemical detection. The results showed that treatment with 200 and 400 ug/kg of triptolide significantly reduced serum levels of E-2 and P and increased levels of FSH and LH. At these dose levels, relative weights of ovary and uterus were significantly reduced. Qualitative histological analysis of the ovaries revealed a reduction in developing follicles and increase in atretic follicles in treated animals. Further, estrous cycles were prolonged significantly. The expression of ER alpha in ovaries and the uterus decreased in treated animals. These data suggest that triptolide had a direct effect on the ovaries, and the decrease in steroids elicited feedback on FSH and LH. Thus, reduced levels of E-2 and P may affect follicular development, estrous cycle, and expression of ERa.

: [Liu, Jing; Jiang, Zhenzhou; Liu, Li; Zhang, Yun; Zhang, Shuang; Xiao, Jingwei; Ma, Min; Zhang, Luyong] China Pharmaceut Univ, Jiangsu Ctr Drug Screening, Nanjing 2100381, Peoples R China; [Jiang, Zhenzhou] China Pharmaceut Univ, Key Lab Drug Qual Control & Pharmacovigilance, Nanjing 2100381, Peoples R China; [Liu, Li] Yangzhou Univ, Sch Med, Dept Pharmacol, Yangzhou, Peoples R China; [Zhang, Luyong] China Pharmaceut Univ, JiangSu Ctr Pharmacodynam Res & Evaluat, Nanjing 2100381, Peoples R China

: Zhang, LY, China Pharmaceut Univ, Jiangsu Ctr Drug Screening, 24 Tong Jia Xiang, Nanjing 2100381, Peoples R China.

: [email protected]

:

Traditional Chinese Medicine for Public Interest Research from Ministry of Finance, China

200707008

Ministry of Science and Technology, China

2004AA2Z3785

New Century Excellent Talents in University

NCET-040507

The program is supported by specific funding from the Traditional Chinese Medicine for Public Interest Research from Ministry of Finance of P. R. China (No. 200707008), Mega-projects of Science Research for 10th Five-year Plan (No. 2004AA2Z3785) from the Ministry of Science and Technology of P. R. China, and New Century Excellent Talents in University (NCET-040507). The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.

: 39

: 0

: TAYLOR & FRANCIS INC

: 325 CHESTNUT ST, SUITE 800, PHILADELPHIA, PA 19106 USA

ISSN: 0148-0545

DOI: 10.3109/01480541003774358

29 : DRUG CHEM TOXICOL

ISO : Drug Chem. Toxicol.

: 7

: Chemistry, Multidisciplinary; Pharmacology & Pharmacy; Toxicology

ISI : 688VC

18 415

: Wang, HY (Wang, Hongyu); Zhu, P (Zhu, Peng)

: Local Riemann-Roch theorem for almost Hermitian manifolds

: BULLETIN OF THE BRAZILIAN MATHEMATICAL SOCIETY, 41 (4): 583-605 DEC 2010

: English

: Article

: almost Hermitian manifold; almost Kahler manifold; Dirac operator; local index

KeyWords Plus: INDEX THEOREM; OPERATORS

: In this paper, we give a local Riemann-Roch theorem for certain almost Hermitian manifolds by a canonical connection and the theory of almost Hermitian structure of null eccentricity. In particular, we obtain a local Riemann-Roch theorem for all closed almost complex manifolds of dimension 4. Also, by using the heat equation method, we give an analytic approach to a local index theorem for generalized Dirac operators.

: [Wang, Hongyu; Zhu, Peng] Yangzhou Univ, Sch Math Sci, Yangzhou 225002, Jiangsu, Peoples R China

: Wang, HY, Yangzhou Univ, Sch Math Sci, Yangzhou 225002, Jiangsu, Peoples R China.

: [email protected]

:

NSF (China)

11071208

Tian-Yuan Fund of Mathematics (China)

Yangzhou University

2010CXJ001

Supported by NSF Grant (China) 11071208, Tian-Yuan Fund of Mathematics (China) and Yangzhou University fund 2010CXJ001.

: 31

: 0

: SPRINGER

: 233 SPRING ST, NEW YORK, NY 10013 USA

ISSN: 1678-7544

29 : BULL BRAZ MATH SOC

ISO : Bull. Braz. Math. Soc.

: 23

: Mathematics

ISI : 686YW

19 415

: Wu, XX (Wu, Xue-Xia); Ding, HD (Ding, Hai-Dong); Chen, JL (Chen, Jian-Lin); Zhang, HJ (Zhang, Hong-Juan); Zhu, WM (Zhu, Wei-Min)

: Attenuation of salt-induced changes in photosynthesis by exogenous nitric oxide in tomato (Lycopersicon esculentum Mill. L.) seedlings

: AFRICAN JOURNAL OF BIOTECHNOLOGY, 9 (46): 7837-7846 NOV 15 2010

: English

: Article

: Nitric oxide; photosynthesis; salt stress; Lycopersicon esculentum L.; stomatal conductance; transpiration rate

KeyWords Plus: CHLOROPHYLL FLUORESCENCE; REVERSIBLE INHIBITION; ELECTRON-TRANSPORT; OXIDATIVE STRESS; HIGHER-PLANTS; GAS-EXCHANGE; TOLERANCE; LEAVES; LIGHT; ARABIDOPSIS

: Exogenous sodium nitroprusside (SNP), a NO donor, was applied in this study to investigate the potential role of NO in photosynthetic performance of tomato (Lycopersicon esculentum L. cv. Hufan2560) seedlings under salt-stressed conditions. Exogenous NO alleviated the decrease in dry mass of shoot and root caused by salt stress. In parallel, NO application in salt-stressed plants attenuated the decrease in the photosynthetic parameters such as leaf chlorophyll, net photosynthetic rate (P-N), stomatal conductance (gs), transpiration rate (E), the ratio of variable to maximum fluorescence (Fv/Fm), electron transport rate (ETR), the efficiency of excitation energy capture by open photosystem II (PSII) reaction centers (Fv'/Fm'), and the photochemical quenching coefficient (qP), and counteracted the increase in on-photochemical quenching coefficient (qN). Furthermore, the changes as mentioned above reversed by NO treatment are specific to salt stress since application of NO alone to tomato seedlings without salt stress had slight effects on the tested parameters. The results obtained here demonstrated that the photosynthetic performance was improved by NO application in salt-stressed plants and such an improvement was associated with an enhancement of gas-exchange and the actual PSII efficiency, which revealed an important role of NO in enhancing resistance of plants to salt stress.

: [Wu, Xue-Xia; Ding, Hai-Dong; Zhu, Wei-Min] Shanghai Acad Agr Sci, Inst Hort Res, Shanghai 201106, Peoples R China; [Chen, Jian-Lin; Zhu, Wei-Min] Shanghai Key Lab Protected Hort Technol, Shanghai 201106, Peoples R China; [Zhang, Hong-Juan] Yangzhou Univ, Coll Hort & Plant Protect, Yangzhou 225009, Peoples R China

: Zhu, WM, Shanghai Acad Agr Sci, Inst Hort Res, Shanghai 201106, Peoples R China.

: [email protected]

:

Shanghai Committee of Science and Technology, China

09JC1412700

Shanghai Prospering Agriculture by Science and Technology Plan, China

2006-2-1

We are grateful for the funding supported by Shanghai Committee of Science and Technology, China (Grant No. 09JC1412700) and by Shanghai Prospering Agriculture by Science and Technology Plan, China (Grant No. 2006-2-1).

: 36

: 0

: ACADEMIC JOURNALS

: P O BOX 5170-00200 NAIROBI, VICTORIA ISLAND, LAGOS 73023, NIGERIA

ISSN: 1684-5315

29 : AFR J BIOTECHNOL

ISO : Afr. J. Biotechnol.

: 10

: Biotechnology & Applied Microbiology

ISI : 683NF

20 415

: Yuan, Y (Yuan, Yu); Thome, I (Thome, Isabelle); Kim, SH (Kim, Seok Hwan); Chen, DT (Chen, Duanteng); Beyer, A (Beyer, Astrid); Bonnamour, J (Bonnamour, Julien); Zuidema, E (Zuidema, Erik); Chang, S (Chang, Sukbok); Bolm, C (Bolm, Carsten)

: Dimethyl Sulfoxide/Potassium Hydroxide: A Superbase for the Transition Metal-Free Preparation of Cross-Coupling Products

: ADVANCED SYNTHESIS & CATALYSIS, 352 (17): 2892-2898 NOV 22 2010

: English

: Article

: arylation; catalysis; cross-coupling; dimethyl sulfoxide/potassium hydroxide (DMSO/KOH); superbase; transition metal-free conditions

KeyWords Plus: LIGAND-FREE CONDITIONS; POTASSIUM TERT-BUTOXIDE; CATALYZED C-N; AROMATIC NUCLEOPHILIC-SUBSTITUTION; DIPOLAR APROTIC-SOLVENTS; ARYL HALIDES; BOND FORMATION; BUTYL ALCOHOL; DIARYL ETHERS; COPPER

: Potassium hydroxide (KOH) in dimethyl sulfoxide (DMSO) forms a superbasic medium that allows one to access cross-coupling products from reactions between aryl halides with various sulfur-, oxygen- and nitrogen-based nucleophiles under transition metal-free conditions.

: [Yuan, Yu; Chen, Duanteng] Yangzhou Univ, Coll Chem & Chem Engn, Yangzhou 225002, Peoples R China; [Thome, Isabelle; Kim, Seok Hwan; Beyer, Astrid; Bonnamour, Julien; Zuidema, Erik; Bolm, Carsten] Rhein Westfal TH Aachen, Inst Organ Chem, D-52056 Aachen, Germany; [Kim, Seok Hwan; Chang, Sukbok] Korea Adv Inst Sci & Technol, Dept Chem, Taejon 305701, South Korea; [Kim, Seok Hwan; Chang, Sukbok] Korea Adv Inst Sci & Technol, Mol Level Interface Res Ctr, Taejon 305701, South Korea

: Yuan, Y, Yangzhou Univ, Coll Chem & Chem Engn, Yangzhou 225002, Peoples R China.

: [email protected]

:

Fonds der Chemischen Industrie

Excellence Initiative of the German federal and state governments

RWTH Aachen University

This research was supported by the Fonds der Chemischen Industrie and, in part, by the Cluster of Excellence funded by the Excellence Initiative of the German federal and state governments. We also acknowledge RWTH Aachen University for financing a pre-doctoral Short Term Scientific Stay for S. H. K. and thank Ms. S. Kohlhepp for valuable synthetic contributions.

: 99

: 0

: WILEY-V C H VERLAG GMBH

: PO BOX 10 11 61, D-69451 WEINHEIM, GERMANY

ISSN: 1615-4150

DOI: 10.1002/adsc.201000575

29 : ADV SYNTH CATAL

ISO : Adv. Synth. Catal.

: 7

: Chemistry, Applied; Chemistry, Organic

ISI : 685HS

21 415

: Hu, X (Hu Xing); Wang, W (Wang Wei); Mao, XY (Mao Xiang-Yu); Chen, XB (Chen Xiao-Bing)

: Magnetic and electric properties of Co-doped Bi-5 Ti-3 FeO15 multiferroic ceramics

: ACTA PHYSICA SINICA, 59 (11): 8160-8166 NOV 2010

: Chinese

: Article

: solid state reaction; multiferroic ceramics; remnant magnetization; remnant polarization

KeyWords Plus: BISMUTH TITANATE; BIFEO3; TRANSITION

: Multiferroic ceramics of Bi5Fe1 - xCoxTi3O15(x = 0. 0-0. 6) are synthesized by the conventional solid state reaction, and their microstructure, magnetic and ferroelectric properties are investigated. X-ray diffraction patterns show that a four-layer Aurivillius phase is formed in each sample with a suggested structural transformation at heavy doping of Co ions. The Raman results demonstrate that Co ions enter into the lattice,occupy the B-sites in the BFCT-x and have an effect on the order occupancy of cations at B sites. It is found that the Co ion modification induces remarkable ferromagnetism ( FM) at room temperature with a greatest remanent magnetization (2M(r)) of 2. 3 memu/g at x = 0. 5, which is three orders of magnitude larger than that of Bi5Ti3FeO15( BFTO). All samples with Co doping have ferroelectricity (EM). The remanent polarization (2P(r)) reaches a value of 11 mu C/cm(2) at x = 0. 1, which is about 38. 2% higher than that of BFTO, and then decreases when 0. 2 T) of the BMPR-IB gene in genotype BB compared with AA. The differences of least squares mean (LSM) for litter size between genotypes AA, AB and BB were non-significant (P > 0.05) in Jining Grey goats. For primer P10, three genotypes (CC, CD and DD) were detected in Jining Grey and Wendeng Dairy goats and one genotype (CC) in Inner Mongolia Cashmere goats. Sequencing revealed one mutation (130T -> C) of the BMPR-IB gene in genotype DD compared with CC. The differences of LSM for litter size between genotypes CC, CD and DD were non-significant (P > 0.05) in Jining Grey goats. These results preliminarily showed that the detected loci of the BMPR-IB gene had no significant effect on prolificacy of Jining Grey goats.

: [Chu, M. X.; Di, R.; Cao, G. L.; Feng, T.; Fang, L.; Ma, Y. H.; Li, K.] Chinese Acad Agr Sci, Key Lab Farm Anim Genet Resources & Utilizat, Minist Agr, Inst Anim Sci, Beijing 100193, Peoples R China; [Zhao, X. H.; Wang, J. Y.] Yangzhou Univ, Coll Anim Sci & Technol, Yangzhou 225009, Peoples R China; [Zhang, Y. J.] Hebei Agr Univ, Coll Anim Sci & Technol, Baoding 071000, Peoples R China; [Jin, M.] Liaoning Normal Univ, Coll Life Sci, Dalian 116029, Peoples R China

: Chu, MX, Chinese Acad Agr Sci, Key Lab Farm Anim Genet Resources & Utilizat, Minist Agr, Inst Anim Sci, Beijing 100193, Peoples R China.

: [email protected]

:

National High Technology Research and Development Program of China

2006AA10Z139


30540052

30871773

National Key Basic Research and Development Program of China

2006CB102105

Earmarked fund for Modern Agro-industry Technology Research System of China

nycytx-39

Beijing Natural Sciences Foundation of China

6062023

Special Fund for Basic Scientific Research of Institute of Animal Science

Chinese Academy of Agricultural Sciences

ywf-rc-1

ywf-td-1

2009td-1

This work was supported by National High Technology Research and Development Program of China (2006AA10Z139), National Natural Science Foundation of China (30540052 and 30871773), National Key Basic Research and Development Program of China (2006CB102105), the earmarked fund for Modern Agro-industry Technology Research System of China (nycytx-39), Beijing Natural Sciences Foundation of China (6062023), Special Fund for Basic Scientific Research of Institute of Animal Science, Chinese Academy of Agricultural Sciences (ywf-rc-1, ywf-td-1 and 2009td-1).

: 28

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: SPRINGER


ISSN: 0301-4851

DOI: 10.1007/s11033-010-0062-x

29 : MOL BIOL REP

ISO : Mol. Biol. Rep.

: 7

: Biochemistry & Molecular Biology

ISI : 672IV

50 415

: Gong, WJ (Gong, Weijuan); Xiao, WM (Xiao, Weiming); Qian, L (Qian, Li); Gong, CX (Gong, Chunxiang); Hu, MZ (Hu, Maozhi); Pan, XY (Pan, Xianyuan); Ji, MC (Ji, Mingchun)

: Immobilized MHC class I chain-related protein A synergizes with IL-15 and soluble 4-1BB ligand to expand NK cells with high cytotoxicity ex vivo

: CELLULAR & MOLECULAR IMMUNOLOGY, 7 (6): 477-484 NOV 2010

: English

: Article

: expansion; IL-15; MICA; natural killer; 4-1BBL

KeyWords Plus: NATURAL-KILLER-CELLS; NKG2D RECEPTOR; TUMOR-IMMUNITY; ACTIVATION; EXPRESSION; CANCER; IMMUNOTHERAPY; ENGAGEMENT; APOPTOSIS

: Major histocompatibility complex (MHC) class I chain-related protein A (MICA), which is a ligand for human NKG2D, is expressed by a variety of epithelial tumor cells and promotes the activation of natural killer (NK), CD8(+) and gamma delta-T cells. Although ectopic expression of MICA on tumor cells elicits anti-tumor responses, soluble MICA downregulates the activities of lymphocytes. In this study, we showed that recombinant, immobilized MICA (iMICA) molecules coated on plastic wells weakly promote peripheral NK cell activation, secretion of interferon (IFN)-gamma and degranulation without inducing apoptosis. In addition, iMICA synergized with IL-15 and soluble 4-1BB ligand (s4-1BBL) to expand NK cells 25-to 42-fold in a 13-day culture, whereas NK cells stimulated only with IL-15 and s4-1BBL expanded 10- to 16-fold. In contrast to NK cells expanded by IL-15 and s4-1BBL stimulation, NK cells expanded long term in the presence of iMICA exhibited increased cytotoxicity against leukemia cells. These results suggest that large numbers of NK cells with high cytotoxicity can be generated by stimulation with IL-15 and s4-1BBL in the presence of iMICA and that these cells can be used for adoptive cancer immunotherapy. Cellular & Molecular Immunology (2010) 7, 477-484; doi: 10.1038/cmi.2010.41; published online 27 September 2010

: [Gong, Weijuan; Qian, Li; Gong, Chunxiang; Pan, Xianyuan; Ji, Mingchun] Yangzhou Univ, Dept Immunol, Sch Med, Yangzhou 225001, Peoples R China; [Gong, Weijuan; Xiao, Weiming] Yangzhou Univ, Affiliated Hosp 2, Dept Gastroenterol, Yangzhou 225001, Peoples R China; [Hu, Maozhi] Yangzhou Univ, Testing Ctr, Yangzhou 225001, Peoples R China

: Gong, WJ, Yangzhou Univ, Affiliated Hosp 2, Dept Immunol, Sch Med, Yangzhou 225001, Peoples R China.

: [email protected]

:

National Natural Science Foundation

30400399

30671917

Natural Science Fund of Jiangsu Province

BK2008215

BK2004404

Educational Committee of Jiangsu Province in China

04KJB320162

This work was supported by the National Natural Science Foundation (30400399 and 30671917), the Natural Science Fund of Jiangsu Province (BK2008215 and BK2004404) and the Natural Science Fund of the Educational Committee of Jiangsu Province (04KJB320162) in China.

: 27

: 0

: CHIN SOCIETY IMMUNOLOGY

: 5 DONGDAN SANTIAO, DONGCHEN DISTRICT, BEING, 100005, PEOPLES R CHINA

ISSN: 1672-7681

DOI: 10.1038/cmi.2010.41

29 : CELL MOL IMMUNOL

ISO : Cell. Mol. Immunol.

: 8

: Immunology

ISI : 674DK

51 415

: Shan, D (Shan, Dan); Li, QB (Li, Qing-Bo); Ding, SN (Ding, Shou-Nian); Xu, JQ (Xu, Jia-Qi); Cosnier, S (Cosnier, Serge); Xue, HG (Xue, Huai-Guo)

: Reagentless biosensor for hydrogen peroxide based on self-assembled films of horseradish peroxidase/laponite/chitosan and the primary investigation on the inhibitory effect by sulfide

: BIOSENSORS & BIOELECTRONICS, 26 (2): 536-541 OCT 15 2010

: English

: Article

: Direct electron transfer; Self-assembly; Laponite; Horseradish peroxidase; Sulfide

KeyWords Plus: DIRECT ELECTRON-TRANSFER; DIRECT ELECTROCHEMISTRY; CYANIDE DETECTION; AZURE-B; ELECTROCATALYSIS; NANOCOMPOSITE; VOLTAMMETRY; LAPONITE; OXIDASE; OXIDATION

: Horseradish peroxidase (HRP) was successfully incorporated into the laponite/chitosan (Chit)-modified glassy carbon electrode (GCE) using step-by-step self-assembly. The self-assembly processes were monitored by X-ray photoelectron spectroscopy (XPS) and electrochemical impedance spectra (EIS). Direct electrochemistry and electrocatalysis of the self-assembled HRP were investigated. Cyclic voltammetry of HRP/laponite/Chit/GCE, in anaerobic phosphate buffer solution (0.025 M PBS, pH 7.0), displayed a pair of stable and quasi-reversible peaks at potentials E-pa = 0.024 V and E-pc = -0.132 V vs. SCE, attributed to the HRP-Fe(III)/HRP-Fe(II) redox couple. The electrochemical reaction of the HRP/laponite/Chit/GCE exhibited a surface-controlled electrode process. The electron transfer rate constant was estimated to be 1.82 s(-1). The self-assembled HRP maintained its biological activity and exhibited an excellent electrocatalytic performance for the reduction of H2O2 with fast amperometric response (10 s). broad linear range (2.9 x 10(-5) to 1.4 x 10(-3) M), good sensitivity (19.7 +/- 0.5 mA M-1 cm(-2)) and low detection limit (5 x 10(-6) M) at a signal-to-noise ratio of 3. Moreover, the inhibitory of sulfide to the self-assembled HRP was also investigated. (C) 2010 Elsevier B.V. All rights reserved.

: [Shan, Dan; Li, Qing-Bo; Xu, Jia-Qi; Xue, Huai-Guo] Yangzhou Univ, Sch Chem & Chem Engn, Key Lab Environm Mat & Environm Engn Jiangsu Prov, Yangzhou 225002, Jiangsu, Peoples R China; [Ding, Shou-Nian] Southeast Univ, Sch Chem & Chem Engn, Nanjing 211189, Peoples R China; [Cosnier, Serge] Univ Grenoble 1, CNRS, ICMG FR 2607, Dept Chim Mol UMR 5250, F-38041 Grenoble, France

: Shan, D, Yangzhou Univ, Sch Chem & Chem Engn, Key Lab Environm Mat & Environm Engn Jiangsu Prov, Si Wangting Rd 180, Yangzhou 225002, Jiangsu, Peoples R China.

: [email protected]

:


20773108

20905011

20810102052

University science research project of Jiangsu Province

09KJB150015

Qing Lan Project of Jiangsu Province

Natural Science Foundation of Jiangsu

SBK201022537

Scientific research foundation for the returned overseas Chinese scholars, State Education Ministry

The authors are grateful to the financial supports of National Natural Science Foundation of China (Grant no. 20773108, 20905011 and 20810102052), University science research project of Jiangsu Province (09KJB150015), Qing Lan Project of Jiangsu Province, the Natural Science Foundation of Jiangsu (SBK201022537), and the project sponsored for D. Shan by the scientific research foundation for the returned overseas Chinese scholars, State Education Ministry.

: 45

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: ELSEVIER ADVANCED TECHNOLOGY

: OXFORD FULFILLMENT CENTRE THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND

ISSN: 0956-5663

DOI: 10.1016/j.bios.2010.07.051

29 : BIOSENS BIOELECTRON

ISO : Biosens. Bioelectron.

: 6

: Biophysics; Biotechnology & Applied Microbiology; Chemistry, Analytical; Electrochemistry; Nanoscience & Nanotechnology

ISI : 675CY

52 415

: Zhuang, J (Zhuang, Jing); Deng, DX (Deng, De-Xiang); Yao, QH (Yao, Quan-Hong); Zhang, JA (Zhang, Jian); Xiong, F (Xiong, Fei); Chen, JM (Chen, Jian-Min); Xiong, AS (Xiong, Ai-Sheng)

: Discovery, phylogeny and expression patterns of AP2-like genes in maize

: PLANT GROWTH REGULATION, 62 (1): 51-58 SEP 2010

: English

: Article

: Transcription factor; AP2/ERF; Expression profile; Arabidopsis thaliana; Maize

KeyWords Plus: TRANSCRIPTIONAL REGULATORY NETWORKS; GENOME-WIDE ANALYSIS; ORYZA-SATIVA L; ZEA-MAYS-L; FUNCTIONAL-ANALYSIS; INFLORESCENCE DEVELOPMENT; RESPONSIVE ELEMENT; APETALA2-LIKE GENE; BINDING PROTEINS; SEED DEVELOPMENT

: A transcription factor (TF) is a protein that binds to specific DNA sequences and thereby controls transcription. A large number of TFs are found in plants. The AP2/ERF family of TFs contain the AP2 DNA-binding domain, which play important roles in transcriptional regulation in plant life. To understand the AP2/ERF family TFs of maize, the discovery, phylogeny and expression pattern analysis of AP2-like genes from maize were described. A total of 167 AP2-like genes were identified by in silico cloning using the amino acid sequence of AP2/ERF family TFs from Arabidopsis thaliana as the probe. The maize AP2-like genes identified were orthologous to those TFs in Arabidopsis. The AP2/ERF TFs from maize were classified into four subfamilies (DREB, ERF, AP2 and RAV) and a Soloist. Using the EST information of maize as a source of expression data, those AP2-like genes from maize were detected in different plant tissues.

: [Zhuang, Jing; Yao, Quan-Hong; Xiong, Ai-Sheng] Shanghai Acad Agr Sci, Shanghai 201106, Peoples R China; [Deng, De-Xiang; Xiong, Fei; Chen, Jian-Min] Yangzhou Univ, Yangzhou 225009, Peoples R China; [Zhuang, Jing; Zhang, Jian] Alberta Res Council, Vegreville, AB T9C 1T4, Canada

: Xiong, AS, Shanghai Acad Agr Sci, 2901 Beidi Rd, Shanghai 201106, Peoples R China.

: [email protected]

:

National "973'' Project

2009CB118400

Hi-tech Research and Development Program of China

2006AA10Z117

National key Project of Transgenic Crops of China

2009ZX08002-011B

International Scientific and Technological Cooperation of Canada-China (Shanghai-Alberta)

Shanghai Rising-Star Program

Natural Science Foundation

08QH14021

08ZR1417200

The research was supported by the National "973'' Project (2009CB118400); Hi-tech Research and Development Program of China (2006AA10Z117); National key Project of Transgenic Crops of China (2009ZX08002-011B); International Scientific and Technological Cooperation of Canada-China (Shanghai-Alberta); Shanghai Rising-Star Program and Natural Science Foundation (08QH14021, 08ZR1417200). We thank Dr. Jeff for critical review of the manuscript.

: 58

: 0

: SPRINGER


ISSN: 0167-6903

DOI: 10.1007/s10725-010-9484-7

29 : PLANT GROWTH REGUL

ISO : Plant Growth Regul.

: 8

: Plant Sciences

ISI : 671KE

53 415

: Li, SR (Li, Shi-Rong); Fu, XH (Fu, Xiao-Hua); Batra, RC (Batra, R. C.)

: Free vibration of three-layer circular cylindrical shells with functionally graded middle layer

: MECHANICS RESEARCH COMMUNICATIONS, 37 (6): 577-580 SEP 2010

: English

: Article

: Free vibration; Cylindrical shell; Functionally graded materials; Natural frequency

KeyWords Plus: BOUNDARY-CONDITIONS; DIFFERENTIAL QUADRATURE

: We study free vibrations of a simply supported three-layer circular cylindrical shell with the inner and the outer layers made of the same homogeneous material and the middle layer composed of a functionally graded material. We use Flugge's shell theory to derive governing equations, express mid-plane displacements in terms of trigonometric functions that identically satisfy the boundary conditions, and compute natural frequencies in terms of the geometrical and the material parameters. Computed results show that the fundamental natural frequency decreases with an increase in the radius-to-thickness ratio, and increases with an increase in the ratio of Young's modulus at the mid-surface to that of the outer (or the inner) layer. (C) 2010 Elsevier Ltd. All rights reserved.

: [Li, Shi-Rong] Yangzhou Univ, Sch Civil Sci & Engn, Yangzhou 225009, Jiangsu, Peoples R China; [Fu, Xiao-Hua] Lanzhou Univ Technol, Dept Engn Mech, Lanzhou 730050, Gansu, Peoples R China; [Batra, R. C.] Virginia Polytech Inst & State Univ, Dept Engn Sci & Mech, Blacksburg, VA 24061 USA

: Li, SR, Yangzhou Univ, Sch Civil Sci & Engn, Yangzhou 225009, Jiangsu, Peoples R China.

: [email protected]

:


10872083

Ministry of Education of China

200807310002

This research was supported by the grant number 10872083 from the National Natural Science Foundation of China and the Fund of Ministry of Education of China for the Supervisors of Doctorate Students (200807310002). The authors gratefully acknowledge these financial supports.

: 21

: 0

: PERGAMON-ELSEVIER SCIENCE LTD

: THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND

ISSN: 0093-6413

DOI: 10.1016/j.mechrescom.2010.07.006

29 : MECH RES COMMUN

ISO : Mech. Res. Commun.

: 4

: Mechanics

ISI : 671EX

54 415

: He, SH (He Shenghu); Yan, JF (Yan Jianfeng); Zhang, J (Zhang Jing); Yuan, Y (Yuan Yuan); Yan, FD (Yan Fengdi); Xie, Y (Xie Yong); Chen, S (Chen Shu)

: Diagnostic Value of Adenosine Stress and Rest 99mtc-MIBI Myocardial Perfusion Imaging for Cardiac Syndrome X

: CIRCULATION, 122 (2): E202-E202 P509 JUL 13 2010

: English

: Meeting Abstract

: World Congress of Cardiology Scientific Sessions

: JUN 16-19, 2010

: Beijing, PEOPLES R CHINA

: [He Shenghu; Yan Jianfeng; Zhang Jing; Yuan Yuan; Yan Fengdi; Xie Yong; Chen Shu] Yangzhou Univ, Jiangsu Prov Peoples Hosp, Yangzhou, Peoples R China

: 0

: 0

: LIPPINCOTT WILLIAMS & WILKINS

: 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA

ISSN: 0009-7322

29 : CIRCULATION

ISO : Circulation

: 1

: Cardiac & Cardiovascular Systems; Hematology; Peripheral Vascular Disease

ISI : 624FI

55 415

: Zhu, LQ (Zhu, L. Q.); Ding, XY (Ding, X. Y.); Tao, J (Tao, J.); Wang, JY (Wang, J. Y.); Zhang, XJ (Zhang, X. J.); Wang, XB (Wang, X. B.); Hu, Y (Hu, Y.); Li, HF (Li, H. F.); Chen, KW (Chen, K. W.); Zhu, GQ (Zhu, G. Q.)

: Identification of target cells for Goose parvovirus infection in the immune system organs

: ACTA VIROLOGICA, 54 (3): 211-215 2010

: English

: Article

: Derzsy's disease; Goose parvovirus; immunoassay; PCR

KeyWords Plus: DERZSYS DISEASE VIRUS; VIRAL DISEASE; ADULT MINK; REPLICATION; GOSLINGS; CULTURE; AGENT; B19

: Target cells for Goose parvovirus (GPV) in natural infection are still unknown. In this study, immune system organs namely the spleen, bone marrow, thymus, bursa of Fabricius, and blood of experimentally GPV-infected goslings were examined by an immunoassay and flow cytometry for the presence of viral antigen and by a PCR for viral genome. The results indicated that the virus replicated in some cells of the spleen and bone marrow, but not in peripheral blood lymphocytes (PBLs). These data suggested that some cell populations in the spleen and bone marrow were targets for GPV infection. In addition, the immunoassay used for the detection of GPV was found comparable with a PCR in reliability and sensitivity.

: [Zhu, L. Q.; Tao, J.; Wang, J. Y.; Zhang, X. J.; Wang, X. B.; Zhu, G. Q.] Yangzhou Univ, Coll Vet Med, Yangzhou 225009, Peoples R China; [Ding, X. Y.] NW A&F Univ, Coll Life Sci, Yangling 712100, Peoples R China; [Ding, X. Y.] Chinese Acad Agr Sci, Poultry Inst, Yangzhou 225009, Peoples R China

: Zhu, GQ, Yangzhou Univ, Coll Vet Med, Yangzhou 225009, Peoples R China.

: [email protected]

:

Science and Technology Department of Jiangsu province

BE2008363

Chinese National Science Foundation

30771603

Jiangsu High Education Key Basic Science Foundation

08KJA230002

Ministry of Agriculture of China

200803020

This work was supported by the grant BE2008363 from the Science and Technology Department of Jiangsu province, the grant 30771603 from the Chinese National Science Foundation, the grant 08KJA230002 from the Jiangsu High Education Key Basic Science Foundation, and the grant 200803020 from the Ministry of Agriculture of China.

: 18

: 0

: SLOVAK ACADEMIC PRESS LTD

: PO BOX 57 NAM SLOBODY 6, 810 05 BRATISLAVA, SLOVAKIA

ISSN: 0001-723X

DOI: 10.4149/av_2010_03_211

29 : ACTA VIROL

ISO : Acta Virol.

: 5

: Virology

ISI : 671OV

56 415

: Han, Y (Han Ying); Li, HH (Li Hengheng); Shen, M (Shen Ming); Li, LA (Li Liang); Xu, GY (Xu Guiying)

: In Situ Reducing Synthesis of p-Dodecyloxybenzenmethanamine-protected Hydrophobic Gold Nanoparticles under Microwave Irradiation

: ACTA CHIMICA SINICA, 68 (18): 1813-1820 SEP 28 2010

: Chinese

: Article

: p-dodecyloxybenzenmethanamine; hydrophobic gold nanoparticle; W/O microemulsion; in situ reduction; microwave irradiation

KeyWords Plus: COLLOIDAL GOLD; SYSTEM

: The hydrophobic gold nanoparticles were fabricated through n-butanol reduction in situ under microwave irradiation by using p-dodecyloxybenzenmethanamine (C(12)OBA)/n-butanol/n-heptane/HAuCl4/NaOH(aq.) W/O microemulsion as a microreactor. The C(12)OBA-stabilized gold nanoparticles were characterized by UV-vis, TEM, XRD, FT-IR and CA (contact angle), respectively. The results showed that C(12)OBA, a novel surfactant, could not only take part in the formation of a stable W/O microemulsion, but also be used as a good protecting reagent for gold nanoparticles. The increase of alkalinity within the inner-core of microemulsion enhanced the reducing ability of n-butanol located in the membrane phase. As the amount of HAuCl4 was fixed, the increase of molar ratios of C(12)OBA/gold was beneficial to obtain hydrophobic gold nanoparticles with small-size and higher monodispersity; while the increase of polarity of reaction system led to the increase of size and decline of monodispersity of particles. And thus by this method, the hydrophobic gold nanoparticles with about 5 nm-diameter and high monodispersity could be easily and quickly synthesized.

: [Han Ying; Li Hengheng; Shen Ming; Li Liang] Yangzhou Univ, Coll Chem & Chem Engn, Yangzhou 225002, Peoples R China; [Xu Guiying] Shandong Univ, Educ Minist, Key Lab Colloid & Surface Chem, Jinan 250100, Peoples R China

: Han, Y, Yangzhou Univ, Coll Chem & Chem Engn, Yangzhou 225002, Peoples R China.


: 20

: 0

: SCIENCE CHINA PRESS

: 16 DONGHUANGCHENGGEN NORTH ST, BEIJING 100717, PEOPLES R CHINA

ISSN: 0567-7351

29 : ACTA CHIM SIN

ISO : Acta Chim. Sin.

: 8

: Chemistry, Multidisciplinary

ISI : 671EN

57 415

: Li, JG (Li JinGui); Xing, T (Xing Tao); Wang, L (Wang Lin); Tao, JP (Tao JianPing); Liu, ZP (Liu ZongPing)

: Inhibitory effect of S-nitroso-glutathione on Eimeria tenella oocysts was mainly limited to the early stages of sporogony

: VETERINARY PARASITOLOGY, 173 (1-2): 64-69 OCT 11 2010

: English

: Article

: S-nitroso-glutathione; Eimeria tenella; Oocysts; Sporulation; EtCRK2

KeyWords Plus: NITRIC-OXIDE PRODUCTION; SARCOCYSTIS CRUZI; CHICKENS; SPORULATION; INFECTIONS; SPOROCYSTS; EXPRESSION; MAXIMA; KINASE; CYCLE

: The objective of this study was to evaluate the action of S-nitroso-glutathione (GSNO) on the sporogony and the mRNA expression for EtCRK2 (a cyclin-dependent kinase related protein-2 in Eimeria tenella) during oocyst sporulation. Meanwhile, the effects of GSNO on the sporozoite excystability and infectivity to chickens were also investigated. The results showed that within 10 h after the initial sporulation, the sporulation process could be interrupted by GSNO treatment, and most of the oocysts failed to continue to sporulate. However, if the sporulation lasted more than 12 h, the sporogony process could not be blocked by GSNO. RT-PCR assay suggested that in the early stage of sporulation, the mRNA expression of EtCRK2 was completely inhibited by GSNO. For the infective oocysts or sporocysts treated by GSNO, the proportion of sporozoite excystation decreased significantly by about 20% and 40%, respectively. However, clinical coccidiosis could be observed in all experimental broilers inoculated with the oocysts or sporocysts treated by GSNO or not. The animal test showed that GSNO treatment reduced the infectivity of sporocysts, whereas it did not alleviate that of oocysts to broilers. Taken together, the data indicated that the viability of sporulated E. tenella oocysts could not be inactivated in vitro by GSNO. The inhibiting action of GSNO on oocysts was mainly limited to the early stages of sporulation, and this may partially associate with the inhibition of EtCRK2 mRNA expression. (C) 2010 Elsevier B.V. All rights reserved.

: [Li JinGui; Xing Tao; Tao JianPing; Liu ZongPing] Yangzhou Univ, Sch Vet Med, Yangzhou 225009, Peoples R China; [Wang Lin] Shandong Agr Univ, Coll Anim Sci & Vet Med, Tai An 271018, Shandong, Peoples R China

: Li, JG, Yangzhou Univ, Sch Vet Med, Yangzhou 225009, Peoples R China.

: [email protected]

:

QingLan Project of Jiangsu Province


30300259

This study was sponsored by QingLan Project of Jiangsu Province and supported by the National Natural Science Foundation of China (Grant No. 30300259). The authors thank Prof. Qin AiJian and Dr. ZhanJun Lu and WenJie Jin in Yangzhou university for technical assistance.

: 26

: 0



ISSN: 0304-4017

DOI: 10.1016/j.vetpar.2010.06.022

29 : VET PARASITOL

ISO : Vet. Parasitol.

: 6

: Parasitology; Veterinary Sciences

ISI : 667KI

58 415

: Huang, J (Huang, Jin); Gao, F (Gao, Feng); Tang, XX (Tang, Xiaoxin); Yu, JH (Yu, Jiahui); Wang, DX (Wang, Daxin); Liu, SY (Liu, Shiyuan); Li, YP (Li, Yaping)

: Liver-targeting doxorubicin-conjugated polymeric prodrug with pH-triggered drug release profile

: POLYMER INTERNATIONAL, 59 (10): 1390-1396 OCT 2010

: English

: Article

: Gal-PEG-b-PAMAM-Dox(n); polymeric prodrug; pH-triggered drug release; doxorubicin; liver-targeting

KeyWords Plus: DELIVERY; DENDRIMERS; CYTOTOXICITY; COPOLYMER; MECHANISM; PAMAM; MICE

: The aim of the research presented was to develop a potential liver-targeting prolonged-circulation polymeric prodrug of doxorubicin (Dox) with a pH-triggered drug release profile. In particular, linear dendritic block copolymers composed of polyamidoamine dendrimer (PAMAM) and poly(ethylene glycol) (PEG; number-average molecular weight of 2000 g mol(-1)) with or without galactose (Gal) were synthesized. Dox was coupled to the copolymers via an acid-labile hydrazone linker. These prodrugs, designated Gal-PEG-b-PAMAM-Dox(n) and mPEG-b-PAMAM-Dox(m), showed accelerated Dox release as the pH decreased from 8.0 to 5.6. Cytotoxicity of the prodrugs was lower than that of free Dox due to the gradual drug release nature. Compared to mPEG-b-PAMAM-Dox(m), Gal-PEG-b-PAMAM-Dox(n) showed rather high cytotoxicity against Bel-7402, suggestive of its galactose receptor-mediated enhanced tumor uptake. This galactose receptor-mediated liver-targeted profile was further confirmed by the prolonged retention time in hepatoma tissue monitored using magnetic resonance imaging. Gal-PEG-b-PAMAM-Dox(n) showed better in vivo antitumor efficacy than free Dox, suggesting its great potential as a polymeric antitumor prodrug. (C) 2010 Society of Chemical Industry

: [Huang, Jin; Gao, Feng; Tang, Xiaoxin; Yu, Jiahui] E China Normal Univ, Inst Adv Interdisciplinary Res, Shanghai 200062, Peoples R China; [Huang, Jin] Wuhan Univ Technol, Coll Chem Engn, Wuhan 430070, Peoples R China; [Wang, Daxin] Yangzhou Univ, Subei Hosp Jiangsu Prov, Yangzhou 225001, Peoples R China; [Liu, Shiyuan] Changzheng Hosp, Dept Diagnost Imaging, Shanghai 200003, Peoples R China; [Li, Yaping] Chinese Acad Sci, Shanghai Inst Mat Med, Ctr Drug Delivery Syst, Shanghai 201203, Peoples R China

: Yu, JH, E China Normal Univ, Inst Adv Interdisciplinary Res, Shanghai 200062, Peoples R China.


:

Shanghai Municipality Commission

0852nm03700

0952nm05300

09540709000

10410710000

Chinese Ministry of Science and Technology

2007CB936104

2009CB930300

WUT

The research work was supported by the Shanghai Municipality Commission for Special Project of Nanometer Science and Technology (0852nm03700, 0952nm05300), Shanghai Municipality Commission for Non-governmental International Corporation Project (09540709000), International Corporation Project of Shanghai Municipality Commission (10410710000), the 973 Projects of Chinese Ministry of Science and Technology (2007CB936104 and 2009CB930300), and Self-Determined and Innovative Research Funds of WUT.

: 23

: 0

: JOHN WILEY & SONS LTD

: THE ATRIUM, SOUTHERN GATE, CHICHESTER PO19 8SQ, W SUSSEX, ENGLAND

ISSN: 0959-8103

DOI: 10.1002/pi.2880

29 : POLYM INT

ISO : Polym. Int.

: 7

: Polymer Science

ISI : 666GT

59 415

: Tian, F (Tian, Fang); Lin, DD (Lin, Dandan); Wu, JJ (Wu, Jingjiao); Gao, YA (Gao, Yanan); Zhang, DH (Zhang, Donghui); Ji, MJ (Ji, Minjun); Wu, GL (Wu, Guanling)

: Immune Events Associated with High Level Protection against Schistosoma japonicum Infection in Pigs Immunized with UV-Attenuated Cercariae

: PLOS ONE, 5 (10): Art. No. e13408 OCT 15 2010

: English

: Article

KeyWords Plus: IRRADIATED CERCARIAE; CELL RESPONSES; MANSONI; MICE; VACCINATION; CHINA; EXPRESSION; CYTOKINES; ANTIGEN; MODEL

: Background: The vaccination of radiation-attenuated Schistosoma japonicum cercariae can induce effective protection in artiodactyl, but the immune events related to protective immunity are not fully understood. To provide a paradigm for a human recombinant antigen vaccine, we have undertaken a vaccination and challenge experiment in pigs, which was recognized as an appropriate animal model in this type of study because of their similarity to human in immunology, and investigated the relative immune events induced by the radiation-attenuated S. japonicum cercariae.Methods and Findings: We found that pigs immunized once with 400 mu w UV-irradiated cercariae exhibited 63.84% and 71.82% reductions in worm burden and hepatic eggs respectively. Protective immunity in vaccinated pigs was associated with high level productions of IgM, total IgG, IgG1 and IgG2; IgG2 was significantly increased in the acute infection. IFN-gamma levels could be elicited by immunization. At week 6 post-infection, IFN-gamma, IL-4 and IL-10 levels also showed a dramatic rise synchronously in vaccinated pigs. Moreover, the granzyme b, nk-lysin, ifn gamma, il4 and il10 mRNA levels in early skin-draining lymph nodes of immunized pigs were higher than those in pigs with non-irradiated cercariae infection. In addition, cytotoxicity-related genes in the mesenteric lymph nodes were significantly upregulated in vaccinated pigs in the acute infection.Conclusion/Significance: Our results demonstrated that IFN-gamma and IgG2 antibody production, as well as genes related to cytotoxicity are associated with the high level protection induced by UV-irradiated Schistosoma japonicum vaccine. These findings indicated that optimal vaccination against S. japonicum required the induction of IFN-gamma, IgG2 antibody related to Th1 responses and cytotoxicity effect.

: [Tian, Fang; Lin, Dandan; Wu, Jingjiao; Gao, Yanan; Zhang, Donghui; Ji, Minjun; Wu, Guanling] Nanjing Med Univ, Dept Pathogen Biol & Immunol, Nanjing, Jiangsu, Peoples R China; [Zhang, Donghui; Ji, Minjun; Wu, Guanling] Jiangsu Prov Key Lab Modern Pathogen Biol, Nanjing, Jiangsu, Peoples R China; [Lin, Dandan] Jiangxi Prov Inst Parasit Dis, Nanchang, Jiangxi, Peoples R China; [Tian, Fang] Yangzhou Univ Med Coll, Dept Pathogen Biol & Immunol, Yangzhou, Jiangsu, Peoples R China

: Tian, F, Nanjing Med Univ, Dept Pathogen Biol & Immunol, Nanjing, Jiangsu, Peoples R China.

: [email protected]

:

National Science Foundation of China (NSFC)

30430600

National Basic Research Program of China (973 Program)

2007CB513106

This work was supported by the National Science Foundation of China (NSFC) (Project No. 30430600) and the National Basic Research Program of China (973 Program 2007CB513106). The funders had no role in study design, data collection and analysis decision to publish, or preparation of the manuscript.

: 46

: 0

: PUBLIC LIBRARY SCIENCE

: 185 BERRY ST, STE 1300, SAN FRANCISCO, CA 94107 USA

ISSN: 1932-6203

: e13408

DOI: 10.1371/journal.pone.0013408

29 : PLOS ONE

ISO : PLoS One

: 12

: Biology; Multidisciplinary Sciences

ISI : 665NX

60 415

: Yan, H (Yan, Hua); Yan, HS (Yan, Huishen); Huang, T (Huang, Tao); Li, GC (Li, Guocai); Gong, WJ (Gong, Weijuan); Jiao, HM (Jiao, Hongmei); Chen, HJ (Chen, Hongju); Ji, MC (Ji, Mingchun)

: Application and expression of HSV gG1 protein from a recombinant strain

: JOURNAL OF VIROLOGICAL METHODS, 169 (2): 351-358 NOV 2010

: English

: Article

: Herpes simplex virus; gG1 protein; Expression; Affinity chromatography; ELISA; Vaccines

KeyWords Plus: HERPES-SIMPLEX-VIRUS; GLYCOPROTEIN-G GENE; ENZYME IMMUNOASSAYS; IGG ANTIBODIES; GENITAL HERPES; TYPE-1; INFECTION; REGION; ELISA; PREDICTION

: According to the homologous sequence of glycoprotein G1 (gG1) genes from different strains of herpes simplex virus type 1 (HSV-1), a pair of primers was designed to amplify the gG1 gene fragment by PCR. Both the PCR product and the pGEX-4T-1 vector were digested with EcoR I and Sal I. The gG1 gene fragment was subcloned into the digested pGEX

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