เอทธิลีน (ethylene)
DESCRIPTION
เอทธิลีน (Ethylene). - PowerPoint PPT PresentationTRANSCRIPT
-
(Ethylene)
-
C2H4 28 ..1900 Neljubow 50 Denny (degreen) Denny 2 Climacteric Non-climacteric fruits
-
(Synergism) (Antagonism)
-
-- (S-adenosylmethionine SAM) ATP 5- (5-methylthioadenosine MTA) MTA 5-(5-methylthioribose) 5- CH3-S- (homoserine) 5- Methionine ----------> SAM ----------> ACC ----------> Ethylene
-
SAM ACC - AVG (aminoethoxyvinylglycine 2-amino-4- aminoethoxy-trans-3-butenoic acid) AVG ACC AVG (Pyridoxal phosphate) SAM ACC (Pyridoxal enzyme) ACC synthase 55,000 ACC Synthase SAM (SAM-methylthioadenosine-lyase)
-
ACC (membrane-associated) ACC -(ACC hydroxylase) (Ethylene Forming Enzyme EFE) ACC -- (N-hydroxy ACC) (cyanocarboxylic acid)
-
climacteric pre-climacteric ACC 0.1 m mol/g ACC ACC 6 7 ACC EFE Ethylene forming enzyme EFE EFE (membrane-bound)
-
pre-climacteric pre-climacteric ACC ACC ACC pre-climacteric 12 ACC EFE ACC synthase
-
(Senescence) climacteric ACC ACC ACC ACC ACC EFE
-
1. ACC 8 2. 0-25 30 40 ACC
-
3. (Vinylglycine) (Hydroxylamine analogues) SAM ACC ACC Synthase SAM ACC ACC
-
3.1 (Vinylglycine analogues L-2-amino-4-alkoxyl-trans-3-butenoic acid) AVG ACC Synthase AVG -CHO Schiff base ACC Synthase AVG SAM ACC ACC AVG ACC Streptomyces sp. AVG
-
3.2 (Hydroxylamine analogues) (oximes) AOA ACC synthase AVG AVG AOA ACC ACC ACC
-
2 ACC (Co+2) (Ni+2) oxidative phosphorylation 2,4-(2,4-Dinitrophenol 2,4-DNP) (carbonylcyanide) EFE ACC
-
rhizobitoxine Rhizobium japonicum (metal chelators) EDTA DIECA KCN
-
_________________________________________________________________________________
conversion constant
( ( l ()
. .)
_________________________________________________________________________________
( Navel)
0.5 - 1
0.1 - 1.0
( McIntosh) 2.0 - 13
25.0 - 2,500
2.2
29.0 - 74.0
( Gros Michel)
1.9
0.05 - 2.1
1.8
0.11 - 0.17
3.4
0.3 - 1.96
3.8
0.04 - 3.0
( Elberta)
5.2
0.9 - 20.7
_________________________________________________________________________________
-
________________________________________________________________________________
conversion constant
( ( l ()
. .)
________________________________________________________________________________
( Bosc)
5.0 80
2.0 - 7.0
0.16 - 0.40
3.2 - 4.4
3.6 - 30
2.5
1.0 - 16
0.25 - 0.34 0.25 - 0.75
0.3
2.0
0.42
0.8
________________________________________________________________________________
-
_________________________________________________________________________________
()
_________________________________________________________________________________
> 0.002
(Floral senescence)
0.01 - 0.1
(Epinasty)
0.1
> 0.1
10
Avena
300
Perilla
_________________________________________________________________________________
-
receptor site receptor site in vivo in vitro Kinetic receptor site (Half-maximal) binding site
-
Yang, S.F. 1985.
--------- ACC Primary reaction----->------>-------->Responses
Complex
Exogenous
-
0.01 ml/l 0.1 ml/l (Half-maximal) 10 ml/l (Saturation doses) 2 1 2 autocatalytic
-
autocatalytic 2 1 2 Cherimoya
-
1. 1 ml/l receptor ki 15 ml/l
-
2. (Ag+) Ag+ complex Ag+ Ag+ Ag+ Ag+ competitive inhibitor ligand receptor site Ag+ receptor site complex
-
3. Norbornadiene 2,5-norbornadiene Ki 170 ml/l norbornadiene competitive inhibitor norbornadiene receptor site ligand receptor site
-
3. isocoumarin ipomeamarone 1. 2.
-
4. 72 2 5. (Russet spot) (Polyphenol Oxidase) (Phenolic)
-
6. 0.5 (Sleepiness) 7. (epinasty) 8. 9.
-
1. 2. (Ethephon) 2-chloro-ethylphosphonic acid 2-chloroethyltris-(2-methoxyethoxy) silane Cl-CH2-CH2-PO3-H2 + 2OH-------------> Cl- + CH2=CH2 + H2PO4 + H2O Cl-CH2-CH2-Si(O-CH2-CH2-O-CH3)3 + 2H2O----------> Cl- + CH2 = CH2 + SiO2+30H-CH2- CH2-CH2-O-CH3 + H+
-
3. Ethylene generator -H2O CH3-CH2-OH ----------------> CH2 = CH2 4. climacteric
-
1. Brominated activated charcoal Scrubber activated charcoal dibromoethane activated charcoal Hydrobromic CH2 = CH2 + Br2-----------> B2-CH2-CH2-Br
-
2. Potassium per manganate scrubber H2O CH2 = CH2 + KMnO4---------------> CH2OH-CH2-OH + MnO2 3. Ultraviolet light scrubber Ultraviolet H2O CH2 = CH2 + O3---------------------> 2HCHO
-
1. (Species Cultivar) 3 . Friar, Grand Rosa Laroda . Eldorado, Red Peanut Santa Rosa . Late Santa Rosa
-
2. Climacteric 3. 0-25 30 4. 8
-
5. 6. climacteric rise 7.
-
8. Stress 9. (Growth Regulators) . . IAA IAA Aspartate IAA IAA
-
. (GA) GA GA . Abscisic acid (ABA) ABA ABA