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www.pan-biotech.dewww.pan-biotech.com

Germany!26 years

made in

www.pan-biotech.com Page 3

Overview

1. SerumIntroductionCertificate of SuitabilityBovine SerumFBS Good Product FamilySpecial Fetal Bovine SerumCertificate of AnalysisTreated SerumOther Animal SerumHuman SerumSerum Services

2 - 133

3 - 45

5 - 67

8 - 910 - 11

1112

12 - 132. Serum-free Systems

Introduction & Product NumbersSerum SubstitutesSerum-free Media

14 - 3515 - 1718 - 2021 - 35

3. MediaIntroductionCell Culture MediaCell Specific Media

36 - 7737

38 - 7172 - 77

4. Serum-free Stem Cell MediaIntroductionStem Cell Media

78 - 9179

80 - 915. Reagents

Media SupplementsBuffered Salt SolutionAmino Acids and VitaminsAntibiotics and Antifungal DrugsEnzymes for Cell DissociationAttachment FactorsSeperating SolutionsSeparating Solutions Pre-FilledPAN SL-S Product LineCryo PreservationDisinfectants

92 - 11393

94 - 9697

98 - 99100 - 101102 - 104

105106107108

109 - 1136. Biologicals

IntroductionHuman Cytokines and Growth FactorsOther Species Cytokines and Growth FactorsChemokines

114 - 121115

116 - 118119120

7. Molecular BiologyPolymerasesMolecular Biology Reagents

122 - 133123 - 128129 - 133

8. PANsysIntroductionPANsys 3000

134 - 136135

135 - 136

Dear valued customer

We think that the current biotech industry favors experienced, mid-sized and flexible pro-ducers with a broad product portfolio, strong research and development capabilities, best-in-class quality, strong business partner relationships and a wide geographic reach. During the last 26 years PAN-Biotech demonstrated its strength with innovative, first-class quality products and excellent services for cell culture around the globe.

For you, as users and customers of cell culture products, the supply situation has changed rapidly in first quarter of 2013. Due to several mergers and acquisitions of bigger as well as smaller companies there were frictions and changes in supply chains. The customer prob-lems and frustrations were highlighted with the question(s):Can you deliver / support us / rescue our production...? YES, WE CAN !

We can supply FBS, media, reagents and all other products presented in this catalogueWe can support you We can develop / optimise your media and much more

Our market prediction for 2013 was more frictions and turbulences. Some of the recent ac-quisitions went through the same route: logistic changes, lack of customer responsiveness, delivery problems, price increases, intransparency and so on. The overall results for custo-mers was a limited choice of vendors and huge international conglomerates with reduced customer service.

PAN-Biotech still remains as one of a few privately owned, ownership managed companies. We value our customers, we value their needs and requirements, we value product quality, we value openness and transparency, we value service and commitments, we value trust and partnership. We followed along these values for the last 26 years and we intend to keep these values for the next 26 years.

We thank all our existing customers, our dedicated and highly motivated staff and our sup-pliers and industry partners who made possible

26 years biotech made in Germany by PAN-Biotech

And we cordially welcome all our new customers, OEM partners and interested internati-onal distributors as well as new business partners. We guarantee you the values described above and our personal commitment for excellent products and services.

Yours sincerely

Christian Niewolik & Jens HartmannOwners & Managing Directors

March, 2014PAN-Biotech GmbH

Phone: +49 (0)8543/6016-30 Fax: +49 (0)8543/6016-49www.pan-biotech.de www.pan-biotech.com

[email protected]

Introduction


Serum

The function of serum in cell cultures• Stimulates cell growth, proliferation and differentia-

tion through hormonal factors• Adhesion factors facilitate and enhance cell attach-

ment on culture dishes (bio-matrix)• Transport and binding proteins provide hormones,

minerals and lipids• Inhibition of toxic substances by binding to serum

proteins

Animal serumThe dose of serum added to a cell culture as nutrient sour-ce depends on factors such as cell type, primary culture or cell line, adherent or suspension culture, and usually is in the range of 5 % to 15 % of the total liquid volume and most times used at 10 %.

Serum is produced from animal blood and fetal bovine serum is the most widely used because it contains an especially high amount of growth factors due to its ori-gin – the blood of fetuses is a by-product of slaughtered cattle.

Advantages of PAN-Biotech• Own raw material resources in different coun-

tries: Australia, South Africa, South America (Brazil) and the United States of America (USA)

• Certificate of Suitability (COS) no. R1-CEP 2002-167-Rev 00

• Licensed according to the EU-decree no. 1774/2002 with vet. no. DE 09 275 0001 14

• Every single batch is fully documented – from the country of origin to the end product

• Every process, from collection of raw serum to pro-duction, is specified in standard operating instruc-tions (SOP) which will be provided upon request

• We offer special types of serum: charcoal absor-bed, delipidized, dialyzed, gamma irradiated, heat inactivated and gamma globulin reduced

• Highest production and safety standards for serum manufacturing

• Best references from industry and research• Extensive analyses and tests are presented in Certifi-

cate of Analysis (CoA)

We exclusively use serum from guaranteed BSE-free collection areas. In addition, we can also confirm that South Africa, as a country of origin, is free from scrapie. No serum batches from Great Britain as the country of origin are processed by PAN-Biotech. We warrant the submission of a complete documentation, consisting of a certificate of origin and a veterinary certificate, shipping documents and a certificate of analysis. Furthermore, every single procedure during an individual production process is documented and then summarized in a pro-duction protocol.

Certificate of Suitability

DeclarationThe manufacturing process and quality control testing are performed in accordance with the submitted records and with a suitable quality assurance system in compli-ance with ISO 9001 quality standards. This quality as-surance system verifies traceability and batch consistency. PAN-Biotech conducts internal and external audits for its quality system on an annual basis. In addition, PAN-Bio-tech audits its raw material serum suppliers on a cyclical basis and reviews the facilities, manufacturing processes and documentation for the collection, handling, storage and transport of raw serum. PAN-Biotech is willing to be inspected, in accordance with the relevant legislation, on request of a relevant authority before and/or after being granted a certificate of suitability.

Production site: PAN-Biotech GmbHAm Gewerbepark 1394501 Aidenbach / GERMANY

Serum Hotline: +49(0)8543/6016-55

www.pan-biotech.comPage 4

Chapter 1

Serum

Introduction 3Certificate of Suitability 3-4Bovine Serum 5FBS Good Product Family 5-6Special Fetal Bovine Serum 7Certificate of Analysis 8-9Treated Serum 10-11Other Animal Serum 11Human Serum 12Serum Services 12-13

1 1


Serum


Serum

1 1Bovine Serum

Bovine serum is the blood fraction remaining after the natural coagulation of blood, followed by centrifugation to remove any remaining red blood cells. The production of bovine serum at PAN-Biotech is tightly controlled, from the collection of serum at the slaughterhouse

and throughout the whole production cycle which is performed without exception in our own production facilities in Aidenbach, Germany. All serum lots are virus and mycoplasma tested.

Fetal Bovine Serum, Australia origin 100 ml500 ml

P30-1301P30-1302

Fetal Bovine Serum, South Africa origin 100 ml500 ml

P30-1505P30-1506

Fetal Bovine Serum, South America origin 100 ml500 ml

P30-3305P30-3306

Fetal Bovine Serum, US admissible 100 ml500 ml

P30-1701P30-1702

Fetal Bovine Serum, US origin 100 ml500 ml

P30-1401P30-1402

Fetal Bovine Serum Premium, South Africa origin 100 ml500 ml

P30-1501P30-1502

Fetal Bovine Serum Premium, South America origin 100 ml500 ml

P30-3301P30-3302

Sera Pro, Fetal bovine serum, low Endotoxin, EU approved

100 ml500 ml

P30-5100P30-5500

FBS EU Professional, Filtrated bovine serum, EU approved

100 ml500 ml

P30-8100P30-8500

Bovine Serum, variable origins 100 ml500 ml

P30-0601P30-0602

Calf serum - newborn, variable origins 100 ml500 ml

P30-0401P30-0402

FBS Good Product Family

The FBS Good product family contains specially processed serum products. Serum of selected batches is filtrated and separated into individual components by a sophisticated chromatographic method. The growth promoting components contained in the serum are then combined and restored in a defined process. Compared to conventional fetal bovine serum the FBS Good product family has been shown to support and promote cell growth of many different cell types equally well or even better.

Advantages

• Innovative new products• Minor batch to batch variation• Once tested – always same quality• No batch testing required• No lot reservation required

NEW


Serum

FBS Good Product Family1

By developing FBS Good Biotech we wanted to create a naturally defined serum with a sustained growth promoting property and a higher safety. It is tested according to EMA (also known as EMEA) and Ph. Eur. guidelines for use in the bio-pharmaceutical industry. FBS Good Biotech only contains serum of highest quality from defined countries as specified. It is not blended or enhanced by addition of growth factors or proteins.

FBS Good Biotech advantages

• Tested according to EMEA CPMP/BWP/1793/02• Tested according to Ph. Eur. 01/2008:2262• Very low endotoxin level• Suitable for bio-pharmaceutical processing• Expanded quality control

By developing FBS Good Forte we wanted to create a naturally defined serum with an increased growth promoting property and a higher safety. Therefore, additional growth fortifying compounds have been added to increase cell proliferation. FBS Good Forte only contains serum of highest quality from defined countries as specified. In addition, growth promoting and stabilizing compounds (e.g. proteins, salts, sugars, vitamins) have been added to further enhance the stability of the serum as well as the proliferation of many different cell types.

FBS Good Forte advantages

• Reproducible enhanced growth properties• Very low endotoxin level• Suitable for many different cell types• Continued high quality• No more batch testing required

Fetal Bovine Serum Biotech


Serum

1FBS Good, Filtrated bovine serum, Australia origin 100 ml

500 mlP40-39100P40-39500

FBS Good, Filtrated bovine serum, EU approved 100 ml500 ml

P40-37100P40-37500

FBS Good, Filtrated bovine serum, US origin 100 ml500 ml

P40-38100P40-38500

FBS Good Forte, Filtrated bovine serum with Additive Fortifier, Australia origin

100 ml500 ml

P40-49100P40-49500

FBS Good Forte, Filtrated bovine serum with Additive Fortifier, EU approved

100 ml500 ml

P40-47100P40-47500

FBS Good Forte, Filtrated bovine serum with Additive Fortifier, US origin

100 ml500 ml

P40-48100P40-48500

FBS Good Biotech, Filtrated bovine serum, Australia origin, tested acc. EMEA 1793 and Ph. Eur. 2262*

100 ml500 ml

P40-59100P40-59500

FBS Good Biotech, Filtrated bovine serum, US origin, tested acc. EMEA 1793 and Ph. Eur. 2262*

100 ml500 ml

P40-58100P40-58500

*tested upon request and produced after receipt of order

FBS GoodBy developing FBS Good we wanted to create a naturally defined serum with a sustained growth promoting property and a higher safety. FBS Good only contains serum of highest quality from defined countries as specified. It is not blended or enhanced by addition of growth factors or proteins.

FBS Good advantages

• Reproducible growth properties• Very low endotoxin level• Suitable for a great variety of cells• Continuous quality control

FBS Good Forte FBS Good Biotech

Special Fetal Bovine Serum

Pansera ES, Fetal bovine serum, Australia origin, special designed for embryonic stem cells

100 ml500 ml

P30-2605P30-2606

Pansera ES, Fetal bovine serum, EU approved,special desgined for embryonic stem cells

100 ml500 ml

P30-2601P30-2602

Pansera ES, Fetal bovine serum, US origin, special designed for embryonic stem cells

100 ml500 ml

P30-2608P30-2609

Fetal Bovine Serum Biotech, Australia origin, tested acc. EMEA 1793 and Ph. Eur. 2262*

100 ml500 ml

P40-1301P40-1302

Fetal Bovine Serum Biotech, US origin, tested acc. EMEA 1793 and Ph. Euro. 2262*

100 ml500 ml

P40-1401P40-1402

*tested upon request and produced after receipt of order

Pansera ES

Our specially developed, proprietary processing methodology for serum enables us to offer a special fetal bovine serum for embryonic stem cells (ES).

Advantages

• Reproducible constant growth properties• Improved cloning efficiency• More undifferentiated clones• Permanent strict quality control• No need for further testing of different batches

The bio-pharmaceutical industry is facing a constantly growing demand for high quality, extensively tested fetal bovine serum originating from FDA-approved regions. PAN-Biotech is meeting this demand and offers a new product, Fetal Bovine Serum Biotech, which is tested according to EMA (also known as EMEA) and Ph. Eur. guidelines.

Fetal Bovine Serum Biotech originates exclusively from Australia or the USA, both of which are approved regions by FDA´s CFR. The entire production process is followed by close inspections and quality controls – from the collection of raw material to the final production and sterile filtration procedure, all steps are documented and traceable.

Especially a possible contamination with bovine viruses has to be excluded. Therefore, a multitude of tests is performed to meet highest safety requirements.

This product is tested according to EMEA CPMP/BWP/1793/02 and Ph. Eur. 01/2008:2262 upon request. In addition, EMA/410/01 rev. 3 and EMA/CHMP/ BWP/457920/2012 rev. 1 are also included as guidelines for testing procedures. Besides extensive testing for viral contamination, supplementary sterility testing is performed before, during and after filling of the product.

ApplicationFetal Bovine Serum Biotech is particularly suited for the production of virus, vaccine, monoclonal antibodies, recombinant protein and growth factors, as well as the manufacture of other bio-pharmaceutical products.

NEW

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Serum

1Serum

1



Serum


Serum

1 1Treated Serum

Fetal Bovine Serum, activated charcoal absorbed, EU approved

100 ml500 ml

P30-2301P30-2302

Fetal Bovine Serum, delipidized, EU approved 100 ml500 ml

P30-3401P30-3402

Fetal Bovine Serum, dialyzed, EU approved 100 ml500 ml

P30-2101P30-2102

Fetal Bovine Serum, gamma irradiated, US origin 100 ml500 ml

P30-2008P30-2009

FBS EU Professional, gamma irradiated, EU approved 100 ml500 ml

P30-2081P30-2085

Fetal Bovine Serum, heat inactivated, Australia origin 100 ml500 ml

P30-1905P30-1906

Fetal Bovine Serum, heat inactivated, US origin 100 ml500 ml

P30-1908P30-1909

FBS EU Professional, heat inactivated, EU approved 100 ml500 ml

P30-1981P30-1985

Ultra low IgG, Fetal bovine serum, EU approved 100 ml500 ml

P30-2801P30-2802

Tetracycline-free serum, Fetal bovine serum,EU approved

100 ml500 ml

P30-3601P30-3602

Activated charcoal treated serum Dialyzed serum

Fetal bovine serum is heated in a water bath with dextran and activated charcoal. The activated charcoal, together with the substances bound to it, is then removed by centrifugation and filtration.

Application

• Work involving reduced hormone content (steroids)• Work involving reduced growth factors (prevention

of cell differentiation)• Receptor studies (e. g. estrogens)• Minimizes lot-to-lot variations in serum

Serum is dialyzed with a 10,000 Dalton exclusion membrane against physiological saline solution (alternative DPBS) until the glucose content is below 10 mg/100 ml.

Application

• Radioactive labeling studies• Hormone-free applications• Tests intolerant for small molecules such as

nucleotides (hypoxanthine, thymidine), amino acids (serine, alanine etc.), sugars or metabolites

Delipidized serum

Lipids are removed from serum by affinity chromatography.

Application

• Lipid metabolism studies• Arteriosclerosis research

Gamma irradiated serum

Serum is exposed to irradiation > 25 kGy

Application

• Biopharmaceutical production• Virus production• Vaccine production• Manufacturing of diagnostic products

Heat inactivated serum Ultra low IgG serumThe average IgG level in fetal bovine serum is in the range of 70 to 330 µg/ml. The IgG content in our Ultra low IgG serum is reduced by affinity chromatography (protein-G affinity column) to max. 5 µg/ml. The biological activity of serum is not affected.

Application

• Antibody production• Monoclonal antibodies• Radioactive labeling

Serum is heated for 30 min to 56 °C in a water bath under repeated gentle mixing.

Application

• Measurements of lactate dehydrogenase in the culture supernatant as a marker for cell damage (serum LDH is inactivated by heat)

• Minimizes lot-to-lot variations in serum (all thermo-labile components are removed)

• Studies on vitamins and growth factors• Enhance viral safety, since heat-labile viruses are

inactivated• Tests that do not tolerate presence of complement

(complement destruction) Tetracycline-free serum

Fetal bovine serum is tested for absence of tetracycline using the TET-off system (luciferase).

Application

• TET-on / TET-off regulated gene expression• Transfections• Expression studies

Chicken serum 100 ml500 ml

P30-0301P30-0302

Donkey serum 100 ml500 ml

P30-0101P30-0102

Goat serum 100 ml500 ml

P30-1001P30-1002

Hamster serum 10 ml P30-0210

Horse serum 100 ml500 ml

P30-0701P30-0702

Lamb serum 100 ml500 ml

P30-0801P30-0802

Mouse serum 10 ml100 ml

P30-0200P30-0201

Pig serum 100 ml500 ml

P30-0901P30-0902

Rabbit serum 100 ml500 ml

P30-1101P30-1102

Rat serum 10 ml100 ml

P30-01901P30-01901E

Sheep serum 100 ml500 ml

P30-4101P30-4102

Other Animal Serum

All serum tested for virus and mycoplasma. Other serum upon request.


Serum

Serum testing


Serum

Human serum is manufactured from human plasma by addition of calcium chloride. This results in clotting of the plasma. After removing the clot, the human serum is washed and concentrated by ultra-filtration and finally filtered through a combination of depth- and membrane- filters.

Human Serum1 1Off-the-clot serum (True human serum)Off-the-clot serum is prepared from human whole blood collected without anti-coagulant, allowed to clot at room temperature and then centrifuged to remove the clot. We provide single donor units as well as pooled off-the-clot serum. Off-the-clot serum is filtered through depth and membrane filters before filling.

Human serum 100 ml500 ml

P30-2401P30-2402

Human AB serum 100 ml500 ml

P30-2501P30-2502

Human AB serum (male) 100 ml500 ml

P30-2901P30-2902

Human serum off-the-clot 100 ml500 ml

P30-2701P30-2702

Serum Services

PAN-Biotech offers a variety of services and test procedu-res for your serum. We deliver these services fast and cost efficient, using the latest up-to-date techniques.

Profit from our expertise! If you need further special tes-ting or particular services please contact PAN-Biotech. In most cases we can find a solution.

Special processing of serum lots

Treatment Method

Charcoal absorbedSerum is heated in a water bath with dextran and activated charco-al. The activated charcoal, together with the substances bound to it, is then removed by centrifugation and filtration.

Delipidized Lipids are removed from serum by affinity chromatography

Dialyzed Serum is dialyzed with a 10,000 Dalton exclusion membrane against physiological saline solution

Heat inactivated Serum is heated for 30 min to 56 °C in a water bath under repeated gentle mixing

IgG reduced The IgG content in serum is reduced by affinity chromatography to max. 5 µg/ml. The biological activity of serum is not affected.

Sterile filtered Serum passes a series of filters with decreasing pore sizes. The last filtration step is done with a 0.2 µm pore size sterile filter.

Test MethodAlbumin, Globulins Serum protein electrophoresis

Bacterial count

Detection of total number of viable aerobic germs will be either done by membrane filtration or plate-flush-method or as surface method. The microorganisms are detected as colony forming units per ml (CFU/ml) on Caso agar plates.

Cell growth Growth test of murine myeloma cells (SP2/0-Ag14) and murine fibroblasts (L929)

Cholesterol Colorimetric test (CHOD-PAP)

Cloning efficiency

Murine myeloma cells (SP2/0-Ag14) are plated on microtiter pla-tes (one cell per well). After 7 days of incubation the developed cell colonies are counted (= absolute cloning efficiency). The results are normalized to a previously tested reference serum (= relative cloning efficiency).

Endotoxin Kinetic limulus amoebocyte lysate test (LAL)Glucose Colorimetric test (Trinder reaction)Hemoglobin Determined spectrophotometrically at three different wave lengthsIgG Radial immune diffusion

MycoplasmaThree different detection systems are used: DNA-binding fluore-scence dye (DAPI), microscopic analysis of microbial cultures and test kits which detect mycoplasma specific enzymes

Osmolality Analyzed by freezing point depressionpH value Measured with pH-electrode

Plating efficiency

Murine fibroblasts (L929) are plated into a Petri dish. After 14 days of incubation the fixed cell colonies are stained with Giemsa and counted (= absolute plating efficiency). The results are normalized to a previously tested reference serum (= relative plating efficien-cy).

SterilityThe absence of bacterial or fungal contamination is verified by dual incubation with Caso-Bouillon or Thioglycolat-Bouillon ac-cording to Ph. Eur. at 32 °C and 20 °C

TetracyclineTested by a TET-off system of a CHO-luc cell line. Absence of tetracyline induces expression of luciferase, which is quantified using the luciferase test system from Promega.

Total protein Colorimetric test (Biuret reaction)

Triglycerides Colorimetric test (Trinder reaction)

Virus testingThe following viruses and the presence of their antibodies are tes-ted by cytopathic effect: Bovine viral diarrhoea virus (BVDV), bo-vine herpes virus (BHV 1) and parainfluenza virus (PI-3)

Virus testing according EMEA guidelinesThe following virus tests are performed according to EMEA guideline CPMP/BWP/1793/02:• Bluetongue and related orbi viruses• Bovine adenovirus• Bovine parvovirus

• Bovine respiratory syncytial virus (BRSV)• Bovine viral diarrhoea virus (BVDV)• Rabies virus (rabies)• Reo virus• Bovine polyoma virus (BPyV)

www.pan-biotech.comPage 38 www.pan-biotech.com Page 39

MediaChapter 3

Introduction

You only want highest quality media for cell cultivation?

At PAN-Biotech, perfect raw materials combined with state-of-the-art technologies guarantee a first-class quality of our media.

Water is the most important component of liquid media, which is why water purity is of outstanding importance for the quality of media. The water we use generally has a very low endotoxin level of < 0.005 EU/ml, and therefore is of highest purity.

Our media are placed in quarantine until quality control procedures are finished. This guarantees an excellent quality of the final product.

Advantages of our cell culture media

• Raw materials used are tested according to the highest possible quality standards

• Standard filling in sterile, high-class PET bottles• Batches of 10 litre up to 4000 litre• Custom service of product optimisation and further

development for specific applications and purposes• CE-label according to medical product law available

upon request

Other sizes and custom formulation

Almost all media available from PAN-Biotech can be filled in special containers as per customer requirement. Besides standard bottles in 100, 500, and 1000 ml, medium can be filled in cans (up to 10 L), bags (up to 500 L), or other containers with fittings according to customer specifications for special applications such as continous feed process or production purposes.

Delivery time

Standard media:In principle within 3 working days in Germany; otherwise we will inform you.Special media and custom products:Within Germany in 4 to 6 weeks after receipt of order.

Shelf life

Powder media 2 yearsLiquid media without Glutamine 2 yearsLiquid media with stable Glutamine 2 yearsLiquid media with L-Glutamine 1 year

Liquid media with L-Glutamine can be used also after the expiry date, but have to be supplemented with new L-Glutamine in this case. Shelf life starts on date of production!

Storage

Powder media 2 – 8° CLiquid media 2 – 8° C protected from light

Benefit from the experience and know-how of PAN-Biotech. Our state-of-the-art production facilities, with a production line specifically installed for these requirements, allow us to produce the formulations especially developed for your needs in constant high quality also for longer periods of time, and to make batch sizes adapted to your need. Our team of scientists will be pleased to advise you regarding your proprietary formulation.

For further information regarding the dependency of ph-values in media on the CO2 concentration in the incubator please refer to our website at www.pan-biotech.com.

3

MediaIntroduction 37Alpha MEM 38-39BME with Hank´s Salts 40BME with Earle´s Salts 40CMRL-1066 Medium 41Dulbecco´s Modified Eagle Medium 42-45DMEM / F12 46-47Glasgow MEM (BHK21) 48Grace´s Insect Medium 49Ham´s F10 Medium 50Ham´s F12 Medium 51Iscove´s Modified Dulbecco´s Medium 52-53IPL-41 Insect Medium 54Joklik-MEM 55Leibovitz´s L-15 Medium 56MC Coy´s 5A Medium 57MCDB Medium 58Medium 199 with Earle´s Salts 59Medium 199 with Hank´s Salts 60MEM with Earle´s Salts 61-62MEM with Hank´s Salts 63RPMI 1640 64-66Schneider´s Drosophila Medium 67TC 100 Insect Medium 68TNM-FH Medium 69Waymouth´s MB 752/1 Medium 70William´s Medium E 71Endopan 3 72Endopan MV 72Endopan PRO 73Pantum 74Hepatopan 74Melanopan 74Neuropan 75Stempan 75EMEM Fibroblasts 75Amniopan 76Amniopan S2 76Marrowpan 77Marrowpan S2 77

3


Media


Media

Alpha MEMComposition

Components mg/LInorganic Salts

Calcium chloride x 2H2OMagnesium sulfate driedPotassium chlorideSodium chlorideSodium dihydrogen phosphate x H2O

264.92139.52400.00

6,800.00140.00

OtherCompo-nents

D(+)-Glucose anhydrousHepesLipoic acidPhenol redSodium pyruvate

1,000.005,958.00

0.2010.00

110.00AminoAcids

L-AlanineL-Arginine x HClL-Asparagine x H2OL-Aspartic acidL-Cysteine x HCl x H2OL-CystineL-GlutamineL-Glutamic acidGlycineL-Histidine x HCl x H2OL-IsoleucineL-LeucineL-Lysine x HClL-MethionineL-PhenylalanineL-ProlineL-SerineL-ThreonineL-TryptophanL-TyrosineL-Valine

25.00126.64

50.0030.00

100.0024.00

292.0075.0050.0042.0052.4052.4072.4715.0032.0040.0025.0048.0010.0036.2046.00

Vitamins L-Ascorbic acidD(+)-BiotinD-Calcium pantothenateCholine chlorideFolic acidmyo-InositolNicotinamidePyridoxal x HClRiboflavinThiamine x HClVitamine B12

50.000.101.001.001.002.001.001.000.101.001.33

Ribonu-cleosides

AdenosineCytidineGuanosineUridine

10.0010.0010.0010.00

Deoxy-ribonu-cleosides

2’-Deoxyadenosine x H2O2’-Deoxycytidine x HCl2’-Deoxyguanosine2’-Deoxythymidine

10.0011.0010.0010.00

Powder Media

Alpha MEM Eagle(1) 10 L P03-2410without L-Glutamine 50 L P03-2450with Ribonucleosideswith Deoxyribonucleosides without NaHCO3 Alpha MEM Eagle(1) 10 L P03-2510with L-Glutamine 50 L P03-2550with Ribonucleosideswith Deoxyribonucleosides without NaHCO3

Alpha MEM Eagle(1) 10 L P03-2310with L-Glutamine 50 L P03-2350without Ribonucleosideswithout Deoxyribonucleosideswith 2.2 g/L NaHCO3

Alpha MEM Eagle(1) 10 L P03-2610with L-Glutamine 50 L P03-2650with 25 mM Hepes with Ribonucleosideswith Deoxyribonucleosideswithout NaHCO3

Alpha MEMDescriptionAlpha MEM is a different formulation of MEM Eagle and contains a higher concentration of amino acids. It also has a higher concentration of lipoic acid, vitamins and pyruvate. Primarily it was developed for the cultivation of hamster kidney cells, but today it is used for a broad range of mammalian cells. Among others the alpha MEM promotes the growth and progeny of bone marrow cells in suspension culture and monolayer. A further possibility is the use as a separation medium or for the out-breeding of amniotic cells.

Liquid Media

Alpha MEM Eagle(1)

without L-Glutaminewithout Ribonucleosideswithout Deoxyribonucleosideswith 2.2 g/L NaHCO3 500 ml P04-21050 Alpha MEM Eagle(1)

with L-Glutamine with Ribonucleosideswith Deoxyribonucleosideswith 2.2 g/L NaHCO3 500 ml P04-21500

Alpha MEM Eagle(1)

with stab. Glutaminwith Ribonucleosideswith Deoxyribonucleosideswith 2.2 g/L NaHCO3 500 ml P04-21250

Special Media

Alpha MEM Eagle(2)

without L-Glutaminewith Ribonucleosideswith Deoxyribonucleosideswith 2.2 g/L NaHCO3 500 ml P04-21150

Alpha MEM Eagle(2)

with L-Glutaminewithout Ribonucleosideswithout Deoxyribonucleosideswith 2.2 g/L NaHCO3 500 ml P04-21060

Alpha MEM Eagle (2)

with stab. Glutaminewithout Ribonucleosidswithout Deoxyribonucleosidswith 2.2 g/L NaHCO3 500 ml P04-21350

Alpha MEM Eagle (2)

with L-Glutaminewithout Glucosewith Ribonucleosideswith Deoxyribonucleosideswith 2.2 g/L NaHCO3 500 ml P04-21502

Alpha MEM Eagle (2)

without Glutaminewithout Phenol redwithout Ribonucleosideswithout Deoxyribonucleosideswith 2.2 g/L NaHCO3 500 ml P04-21051

3 3

(1) usually on stock, (2) minimum order 10 l, (3) available upon request (1) usually on stock, (2) minimum order 10 l, (3) available upon request

www.pan-biotech.com

Media

CMRL-1066 MediumCompositionDescription

The CMRL is a nucleosid- and vitamin-rich medium. In the past it was developed to clone monkey-kidney cells and as long time culture medium for L-cells. It is suitable for many types of human and monkey cells and also for other mammalian cells, especially by using horse and calf serum.

Liquid Media

CMRL – 1066(1)

without L-Glutaminewithout Phenol redwith 2.2 g/L NaHCO3 500 ml P04-84600

Special Media

CMRL – 1066(2)

with L-Glutaminewithout Phenol redwith 2.2 g/L NaHCO3 500 ml P04-84500

Media

Page 42 www.pan-biotech.com Page 43

Media


Calcium chloride x 2H2OPotassium chlorideMagnesium sulfate driedSodium acetate anhydrousSodium chloridedi-Sodium hydrogen phosphate x H2O

264.92400.00139.55

50.006,799.00

139.75

OtherCompo-nents

CholesterolD(+)-Glucose anhydrousGlutathione (red.)Sodium glucuronate x H2OTween 80

0.201,000.00

10.004.005.00

Coenzy-me

Cocarboxylase x HClCoenzyme A Trilithiumsalt x 2H2ONADNADP sodium saltUTP

1.002.60

7.001.001.00

AminoAcids

L-AlanineL-Arginine x HClL-Aspartic acidL-Cysteine x HCl x H2OL-CystineL-GlutamineL-Glutamic acidGlycineL-Histidine x HCl x H2OL-HydroxyprolineL-IsoleucineL-LeucineL-Lysine x HClL-MethionineL-PhenylalanineL-ProlineL-SerineL-ThreonineL-TryptophanL-TyrosineL-Valine

25.0070.0030.00

260.0020.00

100.0075.0050.0020.0010.0020.0060.0070.0015.0025.0040.0025.0030.0010.0040.0025.00

Vitamins L-Ascorbic acidD(+)-BiotinD-Calcium pantothenateCholine chlorideFolic acidmyo-InositolNicotinamidePyridoxal x HClRiboflavinThiamine x HClVitamine B12

50.000.101.001.001.002.001.001.000.101.001.33

Deoxy-ribonu-cleosides

2’-Deoxyadenosine x H2O2’-Deoxycytidine x HCl2’-Deoxyguanosine2’-Deoxythymidine

10.0011.0010.0010.00

BME with Earle´s SaltsDescriptionIn the fifties of the last century it was found that mammalian cells do not only need the 10 essential amino acids, but also cystine, tyrosine and glutamine. In addition to these three amino acids BME includes

When 5,958.00 mg/L HEPES is included there is only 6,300.00 mg/L sodium chloride.

Composition

Liquid Media

BME with HBSS(1)

without L-Glutaminewith 0.35 g/L NaHCO3 500 ml P04-26050

When 5,958.00 mg/L HEPES is included there is only 7,500.00 mg/L sodium chloride. Liquid Media

BME with EBSS(1)


Special Media

BME with EBSS(2)

with L-Glutaminewith 2.2 g/L NaHCO3 500 ml P04-25500

BME with Hank´s Salts


Calcium chloride x 2H2O Magnesium sulfate driedPotassium chlorideSodium chlorideSodium dihydrogen phosphate x H2O

264.92139.52400.00

6,800.00140.00

OtherCompo-nents

D(+)-Glucose anhydrousHepesPhenol red

1,000.005,958.00

10.00AminoAcids

L-Arginine x HClL-CystineL-GlutamineL-Histidine BaseL-IsoleucineL-LeucineL-Lysine x HClL-MethionineL-PhenylalanineL-ThreonineL-TryptophanL-TyrosineL-Valine

21.0012.00

292.008.00

26.0026.0036.47

7.5016.5024.00

4.0018.0023.50

Vitamins D(+)-BiotinD-Calcium pantothenateCholine chlorideFolic acidmyo-InositolNicotinamidePyridoxal x HClRiboflavinThiamine x HCl

1.001.001.001.002.001.001.000.101.00


Calcium chloride x 2H2OMagnesium sulfate driedPotassium chloridePotassium dihydrogen phosphate anhydrousSodium chloridedi-Sodium hydrogen phosphate

185.44139.52400.00

60.00

8,000.0047.88

OtherCompo-nents


1,000.005,958.00

10.00AminoAcids

L-Arginine x HClL-CystineL-GlutamineL-Histidine BaseL-IsoleucineL-LeucineL-Lysine x HClL-MethionineL-PhenylalanineL-ThreonineL-TryptophanL-TyrosineL-Valine

21.0012.00

292.008.00

26.0026.0036.47

7.5016.5024.00

4.0018.0023.50

Vitamins D(+)-BiotinD-Calcium pantothenateCholine chlorideFolic acidmyo-InositolNicotinamidePyridoxal x HClRiboflavinThiamine x HCl

1.001.001.001.002.001.001.000.101.00

Composition

also eight B-vitamins. Originally BME was used for the cultivation of murine L-cells and HeLa cells. With its many variations it is used in many fields of science today. Along with the cultivation of normal mammalian cells BME is very suitable for transformed cells.

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Media

Liquid Media with 4.5 g/L Glucose

DMEM with 4.5 g/L Glucose(1)

without L-Glutaminewithout Sodium pyruvatewith 3.7 g/L NaHCO3 500 ml P04-03500


without L-Glutaminewith Sodium pyruvatewith 3.7 g/L NaHCO3 500 ml P04-03600


with L-Glutaminewithout Sodium pyruvatewith 3.7 g/L NaHCO3 500 ml P04-03550


with L-Glutaminewith Sodium pyruvatewith 3.7 g/L NaHCO3 500 ml P04-03590




with stab. Glutaminewithout Sodium pyruvatewith 3.7 g/L NaHCO3 500 ml P04-04500


with stab. Glutaminewith Sodium pyruvatewith 3.7 g/L NaHCO3 500 ml P04-04510


without L-Glutaminewithout Sodium pyruvate without Phenol redwith 3.7 g/L NaHCO3 500 ml P04-01161


without Glutaminewith Sodium pyruvatewithout Phenol redwith 3.7 g/L NaHCO3 500 ml P04-01158


with L-Glutaminewith Sodium pyruvatewithout Phenol redwith 3.7 g/L NaHCO3 500 ml P04-03591

Media

Page 45Page 44

Special Media with 1.0 g/L Glucose


without L-Glutaminewith Sodium pyruvatewithout Calciumwith 3.7 g/L NaHCO3 500 ml P04-01501

SILAC-DMEM(2)

with 1.0 g/L Glucosewith stab. Glutaminewith Sodium pyruvatewithout L-Argininwithout L-Lysinwith 3.7 g/L NaHCO3 500 ml P04-02501


without L-Glutaminewithout Sodium pyruvatewithout Phenol redwith 3.7 g/L NaHCO3 500 ml P04-03556


without L-Glutaminewith Sodium pyruvatewithout Phenol redwith 3.7 g/L NaHCO3 500 ml P04-01159


with stab. Glutaminewith Sodium pyruvatewithout Phenol redwith 3.7 g/L NaHCO3 500 ml P04-02500S1




with L-Glutaminewithout Sodium pyruvatewith 3.7 g/L NaHCO3 500 ml P04-01555

Dulbecco´s Modified Eagle MediumDulbecco´s Modified Eagle MediumDescriptionIntrinsically developed for the cultivation of murine embryonic cells, DMEM is tailor-made for the cultivation of a broad range of cells, especially if the medium is supplemented with FBS. DMEM is an Eagle medium modification with a four-fold content of amino acids and vitamins. DMEM with 1.0 g/L Glucose is the standard medium, whereas DMEM with 4.5 g/L Glucose is for cells which have a high energy demand.

Liquid Media without Glucose

DMEM without Glucose(1)

without L-Glutaminewithout Sodium pyruvatewith 3.7 g/L NaHCO3 500 ml P04-01548S1




without L-Glutaminewithout Sodium pyruvatewithout Phenol red with 3.7 g/L NaHCO3 500 ml P04-01548

Special Media without Glucose



Powder Media without Glucose


without L-Glutaminewithout Sodium pyruvatewithout Phenol red 10 L P03-0010without NaHCO3 50 L P03-0050

Liquid Media with 1.0 g/L Glucose






with stab. Glutaminewith Sodium pyruvatewith 3.7 g/L NaHCO3 500 ml P04-02500




with L-Glutaminewith Sodium pyruvatewith 25 mM Hepeswith 3.7 g/L NaHCO3 500 ml P04-05551

Powder Media with 1.0 g/L Glucose


with L-Glutaminewith Sodium pyruvate 10 L P03-0510with 3.7 g/L NaHCO3 50 L P03-0550


with L-Glutaminewith Sodium pyruvatewithout Phenol red 10 L P03-01510with 3.7 g/L NaHCO3 50 L P03-01550

Media

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MediaMedia

Dulbecco’s Modified Eagle Medium

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Media


CompositionPowder Media with 4.5 g/L Glucose


without L-Glutamine without Sodium pyruvate 10 L P03-6510without NaHCO3 50 L P03-6550


with L-Glutamine without Sodium pyruvate 10 L P03-0710without NaHCO3 50 L P03-0750


with L-Glutamine with Sodium pyruvate 10 L P03-0810without NaHCO3 50 L P03-0850


with L-Glutaminewithout Sodium pyruvate with 25 mM Hepes 10 L P03-0910without NaHCO3 50 L P03-0950


with L-Glutaminewith Sodium pyruvatewith 25 mM Hepes 10 L P03-1010without NaHCO3 50 L P03-1050

Media

Page 46

Media

Page 47


Calcium chloride anhydrous Iron(III) nitrate x 9H2OMagnesium sulfate anhydrousPotassium chlorideSodium chlorideSodium dihydrogen phosphate anhydrous

200.000.10

97.66400.00

6,400.00108.69

OtherCompo-nents

D(+)-Glucose anhydrousHepesPhenol redSodium pyruvate

4,500.005,958.00

15.00110.00

AminoAcids

L-Arginine x HClL-Cystine x 2HClL-GlutamineGlycineL-Histidine x HCl x H2OL-IsoleucineL-LeucineL-Lysine x HClL-MethionineL-PhenylalanineL-SerineL-ThreonineL-TryptophanL-Tyrosine x 2NaL-Valine

84.0062.58

584.0030.0042.00

104.80104.80146.20

30.0066.0042.0095.2016.00

103.7993.60

Vitamins D-Calcium pantothenateCholine chlorideFolic acidmyo-InositolNicotinamidePyridoxine x HClRiboflavinThiamine x HCl

4.004.004.007.004.004.000.404.00

Dulbecco´s Modified Eagle Medium


with L-Glutaminewith 25 mM Hepeswithout Sodium pyruvatewith 3.7 g/L NaHCO3 500 ml P04-05540


with L-Glutaminewith 25 mM Hepeswith Sodium pyruvatewith 3.7 g/L NaHCO3 500 ml P04-05550

Special Media with 4.5 g/L Glucose


with stab. L-Glutaminewith 25 mM Hepeswithout Sodium pyruvatewith 3.7 g/L NaHCO3 500 ml P04-04550


with L-Glutaminwithout Sodium pyruvatewith 25 mM Hepeswithout Phenol redwith 3.7 g/L NaHCO3 500 ml P04-05545


with stab. Glutaminewith Sodium pyruvatewithout Phenol redwith 3.7 g/L NaHCO3 500 ml P04-03588


without L-Glutaminewith 25 mM Hepeswith Sodium pyruvatewith 3.7 g/L NaHCO3 500 ml P04-01597

DMEM (10 x)(3) with 4.5 g/L Glucosewithout L-Glutaminewithout Sodium pyruvate with NEAAwithout NaHCO3 500 ml P04-03510


without L-Glutaminewith Sodium pyruvate without L-Argininewith 3.7 g/L NaHCO3 500 ml P04-03598


with L-Glutaminewith 25 mM Hepeswithout Sodium pyruvatewith 2.2 g/L NaHCO3 500 ml P04-04057


with L-Glutaminewithout Sodium pyruvatewithout Sodium chloridewithout NaHCO3 500 ml P04-03560


with L-Glutmaminwithout Sodium pyruvatewith 3.7 g/L NaHCO3 500 ml P04-03551


with stab. Glutaminewith Sodium pyruvatewith 25 mM Hepeswithout Phenol redwith 0.5 g/L NaHCO3 500 ml P04-01163


with L-Glutaminewithout Sodium pyruvatewithout Isoleucinewith 3.7 g/L NaHCO3 500 ml P04-03503

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MediaMedia

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Media

DMEM/F12Powder Media

DMEM/F12 (1:1)(1)

without L-Glutamine 10 L P03-6010without NaHCO3 50 L P03-6050

DMEM/F12 (1:1)(1)

with L-Glutamine 10 L P03-1110without NaHCO3 50 L P03-1150

DMEM/F12 (1:1)(1)

with L-Glutaminewith 15 mM Hepes 10 L P03-6110without NaHCO3 50 L P03-6150

DMEM/F12 (1:1)(1)


Media

Page 48

Media

Page 49

Liquid Media

DMEM/F12 (1:1)(1)


DMEM/F12 (1:1)(1)


DMEM/F12 (1:1)(1)

with stab. Glutaminewith 1.2 g/L NaHCO3 500 ml P04-41150

DMEM/F12 (1:1)(1)

without L-Glutaminewith 15 mM Hepeswith 1.2 g/L NaHCO3 500 ml P04-41550

DMEM/F12 (1:1)(1)

with L-Glutaminewith 15 mM Hepeswith 1.2 g/L NaHCO3 500 ml P04-41250

Special Media

DMEM/F12 (1:1)(2)

without L-Glutaminewithout Glucosewith1.2 g/L NaHCO3 500 ml P04-41151

DMEM/F12 (1:1)(2)


DMEM/F12 (1:1)(2)

with L-Glutaminewithout Phenol red with 1.2 g/L NaHCO3 500 ml P04-41650

DMEM/F12 (1:1)(2)

with stab. Glutaminewith 15 mM Hepeswithout Calcium chloridewith 1.2 g/L NaHCO3 500 ml P04-41251

DMEM/F12DescriptionThis medium supports the growth of almost all cell lines. For example, it is used for pancreas cells, Sertoli cells or to culture cells, which are used for human protein production. It combines the advantages of both media DMEM (high concentration of amino acids and vitamins) and Ham‘s F12 (higher concentration of zinc sulphate, putrescine and linoleic acid).

Composition


Calcium chloride x 2H2OIron(III)-nitrate x 9H2OIron(II)-sulfate x 7H2OPotassium chlorideCopper(II)-sulfate x 5H2OMagnesium chlorideMagnesium sulfateSodium chlorideSodium dihydrogen phosphatedi-Sodium hydrogen phosphateZinc sulfate x 7H2O

154.450.050.42

311.830.00128.5748.85

6,999.5054.3570.98

0.43OtherCompo-nents

D(+)-Glucose anhydrousHypoxanthine Linoleic acidDL-68-Lipoic acidSodium pyruvatePhenol redPutrescin x 2HClThymidine

3,151.02.040.04

0.103110.00

8.100.081

0.36AminoAcids

L-AlanineL-Arginine x HClL-Asparagine x H2OL-Aspartic acidL-Cystine x 2HClL-Cysteine x HCl x H2OL-GlutamineL-Glutamic acidGlycineL-Histidine x HCl x H2OL-IsoleucineL-LeucineL-Lysine x HClL-MethionineL-PhenylalanineL-ProlineL-SerineL-ThreonineL-TryptophanL-Tyrosine x 2Na x 2H2OL-Valine

4.45147.35

7.506.65

31.2917.56

365.007.35

18.7531.4854.3758.9691.3717.2435.4817.2726.2553.55

9.0255.8153.00

Vitamins D-(+)-BiotineD-Calcium pantothenateCholin chlorideFolic acidmyo-InositolNicotinamidePyridoxine x HClRiboflavinThiamine x HClVitamine B12

0.0042.128.982.66

12.512.022.030.222.170.68

3 3

(1) usually on stock, (2) minimum order 10 l, (3) available upon request(1) usually on stock, (2) minimum order 10 l, (3) available upon request

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MediaMedia

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Media

Grace´s Insect MediumDescriptionThe Grace‘s Insect Medium was originally developed to culture insect cells including SF9 and SF21 cells. Moreover it supports a broad range of lepidopteran cells.

Composition

Special Media

Grace‘s Insect Medium(2)


Grace’s Insect Medium(2)


Powder Media





Media

Page 50

Media

Page 51


Calcium chloride x 2H2OPotassium chlorideMagnesium chloride x 6H2OMagnesium sulfate drieddi-Sodium hydrogen phosphate

1,324.62,240.002,278.861,939.81

876.92

OtherCompo-nents

DL-Malic acidSuccinic acidFructoseFumaric acidD(+)-Glucose anhydrousα-Ketoglutaric acid sodium saltD-Sucrose

670.0060.00

400.0055.00

700.00425.66

26,680.00AminoAcids

ß-AlanineL-AlanineL-Arginine x HClL-Asparagine x H2OL-Aspartic acidL-CystineL-GlutamineL-Glutamic acidGlycineL-Histidine BaseL-IsoleucineL-LeucineL-Lysine x HClL-MethionineL-PhenylalanineL-ProlineL-SerineL-ThreonineL-TryptophanL-TyrosineL-Valine

200.00200.00700.00350.00350.00

19.18600.00600.00650.00

2,500.0050.0075.00

625.0050.00

150.00350.00550.00175.00100.00

50.00100.00

Vitamins p-Aminobenzoic acidD(+)-BiotinD-Ca-PantothenateCholine chlorideFolic acidmyo-InositolNiacinPyridoxine x HClRiboflavinThiamine x HCl

0.020.010.020.200.020.020.020.020.020.02

Glasgow MEM (BHK 21)DescriptionThe GMEM was developed as a modification of BME to culture primary baby hamster kidney cells. This version has twice the concentration of vitamins and amino acids.

Composition

Liquid Media

Glasgow-MEM (BHK 21)(1)

without L-Glutaminewithout Tryptose phosphate with 2.75 g/L NaHCO3 500 ml P04-97500

Glasgow-MEM (BHK 21)(1) with L-Glutaminewith Tryptose phosphate with 2.75 g/L NaHCO3 500 ml P04-96500

Special Media

Glasgow-MEM (BHK 21)(2) without L-Glutaminewith Tryptose phosphate with 2.75 g/L NaHCO3 500 ml P04-98500

Powder Media

Glasgow-MEM (BHK 21)(1) without L-Glutamine without Tryptose phosphate 10 L P03-3110without NaHCO3 50 L P03-3150

Glasgow-MEM (BHK 21) (1) with L-Glutamine with Tryptose phosphate 10 L P03-6910without NaHCO3 50 L P03-6950

Glasgow-MEM (BHK 21)(1) with L-Glutamine without Tryptose phosphate 10 L P03-6810without NaHCO3 50 L P03-6850

Components w/oTryptosePhosph-ate mg/L

withTrytose

Phosph-ate mg/L

Inor-ganic Salts

Calcium chloride x 2H2OIron(III) nitrate x 9H2OMagnesium sulfate driedPotassium chlorideSodium chloridedi-Sodium hydrogen phosphateSodium dihydrogen phosphate x H2O

264.920.10

139.57400.00

6,400.000.00

124.00

238.430.09

125.64360.00

6,260.00250.00

111.60

OtherCom-po-nents

D(+)-Glucose anhydrousPhenol redPepton from CaseinPepton from meatYeast extract

4,500.0015.00

0.000.000.00

4,250.0013.50

1,000.00500.00500.00

Ami-noAcids

L-Arginine x HClL-CystineL-GlutamineL-Histidine x HCl x H2OL-IsoleucineL-LeucineL-Lysine x HClL-MethionineL-PhenylalanineL-ThreonineL-TryptophanL-TyrosineL-Valine

42.0024.00

292.0021.0052.4052.4073.1015.0033.0047.60

8.0036.2046.80

37.8021.60

262.8018.9047.1647.1665.7913.5029.7042.84

7.2032.5242.12

Vita-mins

D-Calcium pantothenateCholine chlorideFolic acidmyo-InositolNicotinamidePyridoxal x HClRiboflavinThiamine x HCl

2.002.002.003.602.002.000.202.00

1.801.801.803.241.801.800.181.80

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MediaMedia

DescriptionIn the past Ham‘s F12 was the first choice for a serum-free cultivation of CHO-cells and is now substituted through better serum-free systems like our Panserin C6000, which is protein-free in addition. However, Ham‘s F12 is an appropriate medium for mammalian cells when it is supplemented with FBS. It contains a high concentration of vitamins, amino acids and trace elements. The content of zinc sulphate is increased and it contains putrescine and linoleic acid.

Ham´s F12 MediumComposition

Liquid Media

Ham’s F12 Medium(1)






Special Media


with L-Glutaminewithout Phenol redwith 25 mM Hepeswith 1.176 g/L NaHCO3 500 ml P04-14501



Ham´s F12K Medium(2)


Powder Media




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MediaMedia

Page 52

Media

Page 53


Calcium chloride anhydrous Copper(II) sulfate x 5H2OIron(II) sulfate x 7H2OMagnesium chloride x 6H2OPotassium chlorideSodium chloridedi-Sodium hydrogen phosphate anhydrousZinc sulfate x 7H2O

33.300.0030.834

122.00223.657599.9142.04


D(+)-Glucose anhydrousHepesHypoxanthineLinoleic acidDL-Lipoic acidPhenol redPutrescine x 2HClSodium pyruvateThymidine

1801.605958.00

4.080.084

0.211.200.16

110.000.73

AminoAcids

L-AlanineL-Arginine x HClL-Asparagine x H2OL-Aspartic acidL-Cysteine x HCl x H2OL-GlutamineL-Glutamic acidGlycineL-Histidine x HCl x H2OL-IsoleucineL-LeucineL-Lysine x HClL-MethionineL-PhenylalanineL-ProlineL-SerineL-ThreonineL-TryptophanL-TyrosineL-Valine

8.91210.70

15.0113.3135.12

146.2014.71

7.5120.96

3.9413.1236.54

4.484.96

34.5310.5111.91

2.045.44

11.71Vitamins D(+)-Biotin

D-Calcium pantothenateCholine chlorideFolic acidmyo-InositolNicotinamidePyridoxine x HClRiboflavinThiamine x HClVitamin B12

0.0070.24

13.961.32

18.000.0370.0620.038

0.341.36

Ham´s F10 MediumDescriptionHam‘s F10 is an alternative to Ham‘s F12 and it was used primarily to culture CHO-cells. Today, Ham‘s F10 can be used with or without FBS for many different cell cultures. It is used for example for primary cells of rat and chicken, but also for human diploid cells.

Composition


Liquid Media



Special Media

Ham‘s F10 Medium(2)








Powder Media

Ham‘s F-10 Medium(1) without L-Glutamine 10 L P03-5010 without NaHCO3 50 L P03-5050

Ham’s F-10 Medium(1) with L-Glutamine 10 L P03-3910without NaHCO3 50 L P03-3950

Ham’s F-10 Medium(1)

with L-Glutamine with 25 mM Hepes 10 L P03-4010without NaHCO3 50 L P03-4050


Calcium chloride x 2H2OCopper(II) sulfate x 5H2OIron(II) sulfate x 7H2OMagnesium sulfate driedPotassium chloridePotassium dihydrogen phosphateSodium chloridedi-Sodium hydrogen phosphateZinc sulfate x 7H2O

44.090.0030.834

106.06285.00

83.00

7400.00153.70


D(+)-Glucose anhydrousHypoxanthineDL-α-Lipoic acidHepesPhenol redSodium pyruvate2´- Deoxythymidine

1100.004.080.21

5958.001.20

110.000.73

AminoAcids

L-AlanineL-Arginine x HClL-Asparagine x H2OL-Aspartic acidL-Cysteine x HCl x H2OL-GlutamineL-Glutamic acidGlycineL-Histidine x HCl x H2OL-IsoleucineL-LeucineL-Lysine x HClL-MethionineL-PhenylalanineL-ProlineL-SerineL-ThreonineL-TryptophanL-TyrosineL-Valine

8.91211.00

15.0013.3035.12

146.2014,70

7.5121.00

2.6013.1029.30

4.484.96

11.5010.50

3.570.601.813.50

Vitamins D(+)-BiotinD-Calcium pantothenateCholine chlorideFolic acidmyo-InositolNicotinamidePyridoxine x HClRiboflavinThiamine x HClVitamin B12

0.0240.7150.698

1.320.5410.615

0.210.376

1.011.36

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MediaMedia

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Media

Iscove´s Modified Dulbecco´s MediumDescriptionThe IMDM is a modified DMEM, with a higher content of vitamins, selenium and amino acids. As it is supplemented with albumin, transferrin and soy lipids it can be excellently applied for culturing lymphocytes, marrow cells or hybridoma cells. Note: for hybridomas there is a better and highly efficient protein-free medium available: our Panserin H4000.

Composition


Liquid Media

IMDM(1)


IMDM(1)

with L-Glutaminewith 3.024 NaHCO3 500 ml P04-20350

IMDM(1)


IMDM(1) with L-Glutaminewith 25 mM Hepeswith 3.024 g/L NaHCO3 500 ml P04-20150

Media

Page 54 Page 55


Calcium chloride anhydrousPotassium chloridePotassium nitrateMagnesium sulfate anhydrousSodium chlorideSodium dihydrogen phosphate x H2OSodium selenite x 5H2O

165.00330.00

0.07697.66

5,005.00125.00


D(+)-Glucose anhydrousHepesSodium pyruvatePhenol red

4500.005958.00

110.0015.00

AminoAcids

L-AlanineL-Arginine x HClL-Asparagine x H2OL-Aspartic acidL-Cystine x 2HClL-GlutamineL-Glutamic acidGlycineL-Histidine x HCl x H2OL-IsoleucineL-LeucineL-Lysine x HClL-MethionineL-PhenylalanineL-ProlineL-SerineL-ThreonineL-TryptophanL-Tyrosine x 2Na x 2H2OL-Valine

25.0084.0028.4030.0091.24

584.0075.0030.0042.00

105.00105.00146.00

30.0066.0040.0042.0095.0016.00104.294.00

Vitamins D(+)-BiotinD-Calcium pantothenateCholine chlorideFolic acidmyo-InositolNicotinamidePyridoxine x HClRiboflavinThiamine x HClVitamin B12

0.01304.004.004.007.204.004.000.404.00

0.013

Iscove´s Modified Dulbecco´s MediumSpecial Media

IMDM(2) without L-Glutaminewith 1.0 g/L Glucosewith 3.024 g/L NaHCO3 500 ml P04-20259

IMDM(2)

with stab. Glutaminewith 25 mM Hepeswithout Phenol red315 mOsmwith 3.024 g/L NaHCO3 500 ml P04-20451S1

IMDM(2) with L-Glutaminewith 25 mM Hepes320 mOsmwith 3.024 g/L NaHCO3 500 ml P04-20150S2

IMDM(2)


IMDM(2) with stab. Glutaminewith 25 mM Hepeswith 3.024 g/L NaHCO3 500 ml P04-20450

IMDM(2)

with stab. Glutaminewith 25 mM Hepeswithout Phenol redwith 3.024 g/L NaHCO3 500 ml P04-20451

Powder Media

IMDM(1)


IMDM(1) with L-Glutamine 10 L P03-1310without NaHCO3 50 L P03-1350

IMDM(1) with L-Glutaminewith 25 mM Hepes 10 L P03-1410without NaHCO3 50 L P03-14503 3


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MediaMedia

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Media

IPL-41 Insect MediumDescriptionIPL-41 is primarly used for the growth and maintenance of lepidopteran cells and for the propagation of viruses in these cells lines. The medium is also used for long time culture of baculo-virus infected spodotera cells.

Composition

Special Media

IPL-41 Insect Medium(2)


Powder Media

IPL-41 Insect Medium(1)


Media

Page 56 Page 57


Ammonium heptamolybdate x 4H2OCalcium chloride x 2H2OCobalt(II) chloride x 6H2OCopper chloride x 2H2OIron(II) sulfate x 7H2OMagnesium sulfate driedManganese chloride x 4H2OPotassium chlorideSodium chlorideSodium dihydrogen phosphate x H2OZinc chloride

0.04

662.310.050.200.55

1311.400.02

1200.002850,001160.00


Fumaric acidD(+)-Glucose anhydrousα-Ketoglutaric acid sodium saltDL-Malic acidD-Maltose x H2OSuccinic acidSucrose

4.402500.00

34.0553.60

1052.584.80

1650.00AminoAcids

β-AlanineL-Arginine x HClL-Aspartic acidL-Asparagine x H2OL-CystineL-GlutamineL-Glutamic acidGlycineL-Histidine BaseL-HydroxyprolineL-IsoleucineL-LeucineL-Lysine x HClL-MethionineL-PhenylalanineL-ProlineL-SerineL-ThreonineL-TryptophanL-TyrosineL-Valine

300.00800.00

1300.001477.14

100.001000.001500.00

200.00200.00800.00750.00250.00700.00

1000.001000.00

500.00200.00200.00100.00250.02500.00

Vitamins p-Aminobenzoic acidD(+)-BiotinD-Calcium pantothenateCholine chlorideFolic acidmyo-InositolNicotinic acidNicotinamidePyridoxine x HClRiboflavinThiamine x HClVitamine B12

0.320.16

0.00820.00

0.080.400.160.160.400.080.080.24

Joklik-MEMDescriptionJoklik´s MEM is a modification of MEM for suspension cultures. Due to the absence of calcium chloride in this formulation the attachment of cells is reduced.

Composition

Liquid Media

Joklik – MEM(1)

Hepes Mediumwith L-Glutaminewith 3.6 g/L Hepes 500 ml P04-21300

Special Media

Joklik - MEM(2) modified for spinner culturewith EBSS (modified)without L-Glutaminewithout Antibioticawithout Calciumchloridewith 2.0 g/L NaHCO3 500 ml P04-21200

Powder Media

Joklik - MEM(1)

modified for spinner culturewith EBSS (modified)without L-Glutaminewithout Antibioticawithout Calciumchloride 10 L P03-02010Pwithout NaHCO3 50 L P03-02050P


Magnesium chloride x 6H2OPotassium chlorideSodium chlorideSodium dihydrogen phosphate x H2O

200.00400.00

6,500.001,327.00

OtherCompo-nents

D(+)-Glucose anhydrousPhenol red

2,000.0010.00

AminoAcids

L-Arginine x HClL-CystineL-GlutamineL-Histidine BaseL-IsoleucineL-LeucineL-Lysine x H2OL-MethionineL-PhenylalanineL-ThreonineL-TryptophanL-TyrosineL-Valine

126.0024.00

294.0031.0052.0052.0065.0015.0032.0048.0010.0032.6046.00

Vitamins D-Calcium pantothenateCholine chlorideFolic acidmyo-InositolNicotinamidePyridoxal x HClRiboflavinThiamine x HCl

1.001.001.002.001.001.000.101.00

3 3


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MediaMedia

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Media

Leibovitz´s L-15 MediumDescriptionL-15 contains no sodium hydrogen carbonate and no bicarbonate, because it is buffered already by a high concentration of amino acids. The L-15 medium supports the growth of established cells like Hep-2, but also human nerve cells and primary tissue explants. With 10 % tryptose phosphate broth it is also ideally suited for the cultivation of insect cell lines.

Composition

Liquid Media

Leibovitz’s L-15 Medium(1)

without L-Glutaminewithout NaHCO3 500 ml P04-27055

Leibovitz’s L-15 Medium(1) with L-Glutaminewithout NaHCO3 500 ml P04-27500

Special Media

Leibovitz’s L-15 Medium(2) with stab. Glutaminewithout NaHCO3 500 ml P04-27050

Leibovitz’s L-15 Medium(2) without L-Glutaminewithout Phenol redwithout NaHCO3 500 ml P04-27054

Powder Media





Media

Page 58 Page 59


Calcium chloride x 2H2OMagnesium chloride x 6H2OMagnesium sulfate driedPotassium chloridePotassium dihydrogen phosphateSodium chloridedi-Sodium hydrogen phosphate

185.44200.00139.53400.00

60.00

8,000.00190.00

OtherCompo-nents

D(+)-Galactose anhydrousHepesPhenol redSodium pyruvate

900.005,958.00

10.00550.00

AminoAcids

L-AlanineL-Arginine BaseL-Asparagine x H2OL-CysteineL-GlutamineGlycineL-Histidine BaseL-IsoleucineL-LeucineL-Lysine x HClL-MethionineL-PhenylalanineL-SerineL-ThreonineL-TryptophanL-TyrosineL-Valine

225.00500.00250.00120.00300.00200.00250.00250.00125.00

93.7575.00

125.00200.00300.00

20.00300.00100.00

Vitamins D-Calcium pantothenateCholine chlorideFolic acidmyo-InositolNicotinamidePyridoxine x HClRiboflavin-5’-phosphate sodium salt x 2H2OThiamine monophosphate chloride x2 H2O

1.001.001.002.001.001.00

0.1075

1.00


Mc Coy´s 5A MediumDescriptionMc Coy‘s 5A Medium is a complete medium with all amino acids and vitamins. It is used for growing primary cultures. This group contains marrow cells, gingival cells, adrenal cells, spleen cells, lung cells, rat embryos and other cell types.

Composition


Powder Media

McCoy’s 5A Medium (modified)(1) with L-Glutamine 10 L P03-1710without NaHCO3 50 L P03-1750

McCoy’s 5A Medium (modified)(1)

with L-Glutamine with 25 mM Hepes 10 L P03-1810without NaHCO3 50 L P03-1850

Liquid Media

Mc Coy’s 5A Medium (modified)(1)




Special Media



Mc Coy’s 5A Medium(2) without L-Glutaminewithout Phenol redwith 2.2 g/L NaHCO3 500 ml P04-05610


Calcium chloride x 2H2OMagnesium sulfate driedPotassium chlorideSodium chlorideSodium dihydrogen phosphate x H2O

132.46139.53400.00

6,460.00580.00

OtherCompo-nents

D(+)-Glucose anhydrousGlutathione (red.)HepesBacto - PeptonePhenol red

3,000.000.50

5,958.00600.00

10.00AminoAcids

L-AlanineL-Arginine x HClL-Asparagine x H2OL-Aspartic acidL-CysteineL-GlutamineL-Glutamic acidGlycineL-Histidine x HCl x H2OL-HydroxyprolineL-IsoleucineL-LeucineL-Lysine x HClL-MethionineL-PhenylalanineL-ProlineL-SerineL-ThreonineL-TryptophanL-TyrosineL-Valine

13.3642.1045.0019.9724.24

219.2022.10

7.5020.7619.7039.3639.3636.5014.9016.5017.3026.3017.90

3.1018.1017.60

Vitamins p-Aminobenzoic acidAscorbic acidD(+)-BiotinD-Calcium pantothenateCholine chlorideFolic acidmyo-InositolNicotinamideNicotinic acidPyridoxal x HClPyridoxine x HClRiboflavinThiamine x HClVitamin B12

1.000.500.200.205.00

10.0036.00

0.500.500.500.500.200.202.00

3 3


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MediaMedia

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MediaMedia

Page 60 Page 61

DescriptionMCDB 131 is a medium for the cultivation of human micro-vascular endothelial cells under reduced serum content. For this purpose it has be supplemented with dialyzed serum, EGF and hydrocortisone.

Liquid Media

MCDB 131(1) without L-Glutaminewith 1.176 g/L NaHCO3 500 ml P04-80057

Special Media

MCDB 131(2) with L-Glutaminewith 1.176 g/L NaHCO3 500 ml P04-80053

MCDB 131(2)

without Glutamine with 25 mM Hepeswith 1.176 g/L NaHCO3 500 ml P04-80054


Ammonium MetavandateCalcium Chloride x 2H2OCopper(II) Sulfate x 5H2OIron (III) sulfate x 7H2OMagnesiumsulfate driedManganese Sulfate x H2OAmmonium Molybdate x 4H2ONickel Chloride x 6H2OPotassium ChlorideSodium ChlorideSodium Metasilicate x 5H2ODi-Sodium hydrogen phosphateSodium Selenite anhydrousZinc Sulfate x 7H2O

0.0006235.050.0012

0.2831565.20

0.00020.00370.0007298.00

6,430.002.09

71.000.00390.0003

OtherCompo-nents

AdenineD-GlucoseDL-α-Lipoic acid Phenol RedPutrescine x 2HClSodium pyruvate2‘Deoxythymidine

0.1351,000.00

0.002110.000.002

110.000.024

AminoAcids

L-AlanineL-Arginine x HClL-Asparagine x H2OL-Aspartic AcidL-Cysteine x HCl x H2OL-Glutamic AcidL-GlutamineGlycineL-Histidine x HCl x H2OL-IsoleucineL-LeucineL-Lysine x HClL-MethionineL-PhenylalanineL-ProlineL-SerineL-ThreonineL-TryptophanL-TyrosineL-Valine

2.7063.2015.0013.3035.00

4.001,461.00

2.3042.0066.00

131.00182.00

15.0033.0011.5032.0012.00

4.1018.10

117.10Vitamins D-Biotin

Choline ChlorideFolic Acidmyo-Inositol NiacinamideD-Calcium-pantothenate Pyridoxine x HClRiboflavinThiamine x HClVitamin B12

0.007313.96

0.607.206.10

12.002.10

0.00383.40

0.0136

MCDB 131 Medium Medium 199 with Earle´s SaltsDescriptionThe M199 was originally developed to assay the nutrient demand of embryonic chicken fibroblasts. But it works very well with cells from many different animal species. For example, it is used for vaccine production in virology. For long term cultures serum should be added.

Composition


Liquid Media

M199 with EBSS(1)


Special Media

M199 with EBSS(2)


M199 with EBSS(2)


M199 with EBSS(2)


Powder Media

M199 with EBSS(1)



Calcium chloride x 2H2OIron(III) nitrate x 9H2OMagnesium sulfate driedPotassium chlorideSodium acetate x 3H2OSodium chlorideSodium dihydrogen phosphate

264.920.72

139.52400.00

82.956,800.00


Adenine sulfateAMPATPCholesterol2’-DeoxyriboseD(+)-Glucose anhydrousGlutathione (red,)Guanine x HClHepesHypoxanthinePhenol redD-RiboseThymineTween 80UracilXanthine

10.000.201.000.200.50

1,000.000.050.30

5,958.000.30

10.000.500.304.900.300.30

AminoAcids


25.0070.0030.00

0.1020.00

100.0067.0050.0021.8810.0020.0060.0070.0015.0025.0040.0025.0030.0010.0040.0025.00

Vitamins p-Aminobenzoic acidAscorbic acidD(+)-BiotinCalciferolD-Calcium pantothenateCholine chlorideFolic acidmyo-InositolMenadioneNicotinic acidNicotinamidePyridoxal x HClPyridoxol x HClRiboflavinDL-α-Tocopherol phosphate- Na2Thiamine x HClVitamin A acetate

0.050.050.010.100.010.500.010.050.01

0.0250.0250.0250.025

0.010.01

0.010.14

3 3


Composition

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Medium 199 with Hank´s SaltsSpecial Media

M199 with HBSS(2)


M199 with HBSS(2)


M199 with HBSS (10X)(2)


Powder Media

M199 with HBSS(1)

with L-Glutamine 10 L P03-2110 without NaHCO3 50 L P03-2150


MediaMedia

MEM with Earle´s SaltsDescriptionMEM is an advancement of the BME and the base medium of many further modifications. Because BME did not fulfil all requirements for some mammalian and HeLa cells, a better variation had to be developed. Today, MEM is one of the most used synthetic media and shows its versatility by supplementing with amino acids including Hank´s or Earle´s salts. Even the addition of only small amounts of FBS results in a positive effect on cell growth.

Composition


Liquid Media without Glutamine

MEM Eagle with EBSS(1)


MEM Eagle with EBSS(1) without L-Glutaminewith 25 mM Hepeswith 2.2 g/L NaHCO3 500 ml P04-08150

Special Media without Glutamine




without L-Glutaminewith NEAAwith 2.2 g/L NaHCO3 500 ml P04-08509



Powder Media without Glutamine



Page 62

Media

CompositionComponents mg/L

Inorganic Salts

Calcium chloride x2H2OIron(III) nitrate x 9H2OMagnesium sulfate driedPotassium chloridePotassium dihydrogen phosphateSodium acetate x 3H2OSodium chloridedi-Sodium hydrogen phosphate

185.450.72

139.68400.00

60.00

83.008,000.00


Adenine sulfateAMPATPCholesterol2’-DeoxyriboseD(+)-Glucose anhydrousGlutathione (red.)Guanine x HClHepesHypoxanthinePhenol redD-RiboseThymineTween 80UracilXanthine

10.000.201.000.200.50

1,000.000.050.30

5,958.000.30

10.000.500.304.900.300.30

AminoAcids


25.0070.0030.00

0.1020.00

100.0067.0050.0021.8810.0020.0060.0070.0015.0025.0040.0025.0030.0010.0040.0025.00

Vitamins p-Aminobenzoic acidAscorbic acidD(+)-BiotinCalciferolD-Calcium pantothenateCholine chlorideFolic acidmyo-InositolMenadioneNicotinic acidNicotinamidePyridoxal x HClPyridoxol x HClRiboflavinDL-α-Tocopherol phosphate- Na2Thiamine x HClVitamin A acetate

0.050.050.010.100.010.500.010.050.01

0.0250.0250.0250.025

0.010.01

0.010.14


Calcium chloride x2H2OMagnesium sulfate driedPotassium chlorideSodium chlorideSodium dihydrogen phosphate x H2O

264.92139.53400.00

6800.00140.00

OtherCompo-nents


1000.005958.00

10.00AminoAcids

L-AlanineL-Arginine x HClL-Asparagine x H2OL-Aspartic acidL-CystineL-GlutamineL-Glutamic acidGlycineL-Histidine x HCl x H2OL-IsoleucineL-LeucineL-Lysine x HClL-MethionineL-PhenylalanineL-ProlineL-SerineL-ThreonineL-TryptophanL-TyrosineL-Valine

8.90126.00

13.2013.3024.00

292.0014.70

7.5042.0052.0052.0072.5015.0032.0011.5010.5048.0010.0036.0046.00

Vitamins D-Calcium pantothenateCholine chlorideFolic acidmyo-InositolNicotinamidePyridoxal x HClRiboflavinThiamine x HCl

1.001.001.002.001.001.000.101.00

3 3


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Media

Page 64

MEM with Earle´s SaltsLiquid Media with L-Glutamine





Special Media with L-Glutamine


with L-Glutaminewithout Phenol redwith 1.5 g/L NaHCO3 500 ml P04-00508




with L-Glutaminewith NEAAwith 2.2 g/L NaHCO3 500 ml P04-08510


with 2 mM Glutaminewith 1 mM Pyruvatewith NEAAwith 1.5 g/L NaHCO3 500 ml P04-08056


MediaMedia

MEM with Hank´s SaltsComposition


Special Media

MEM Eagle with HBSS(2)






Powder Media



Powder Media with L-Glutamine


with L-Glutaminewith NEAA 10 L P03-2910without NaHCO3 50L P03-2950


with L-Glutaminewith NEAA with 25 mM Hepes 10 L P03-3010with NaHCO3 50 L P03-3050





Liquid Media with stab. Glutamine




with stab. Glutaminewith 25 mM Hepeswith 2.2 g/L NaHCO3 500 ml P04-08250


Calcium chloride x 2H2OPotassium chloridePotassium dihydrogen phosphate anhydrousMagnesium sulfate driedSodium chloridedi-Sodium hydrogen phosphate

185.44400.00

60.00

139.538,000.00



1,000.005,958.00

10.00AminoAcids

L-AlanineL-Arginine x HClL-Asparagine x H2OL-Aspartic acidL-CystineL-GlutamineL-Glutamic acidGlycineL-Histidine x HCl x H2OL-IsoleucineL-LeucineL-Lysine x HClL-MethionineL-PhenylalanineL-ProlineL-SerineL-ThreonineL-TryptophanL-TyrosineL-Valine

8.90126.00

13.2013.3024.00

292.0014.70

7.5042.0052.0052.0072.5015.0032.0011.5010.5048.0010.0036.0046.00

Vitamins D-Calcium pantothenateCholin chlorideFolic acidmyo-InositolNicotinamidePyridoxal x HClRiboflavinThiamine x HCl

1.001.001.002.001.001.000.101.00

3 3


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Media

Page 66

RPMI 1640DescriptionThe medium was developed for culture of normal and neoplastic leukocytes, but also marrow cells and hybridoma cells. Meanwhile there are better, serum-free media for hybridoma cells such our Panserin H4000. Just by supplementing RPMI 1640 with varying amounts of FBS, a very good medium for many different cell lines can be obtained.

Composition


Liquid Media without Glutamine

RPMI 1640(1)


RPMI 1640(1) without L-Glutaminewithout Phenol redwith 2.0 g/L NaHCO3 500 ml P04-16516

RPMI 1640(1)



MediaMedia

RPMI 1640Special Media without Glutamine

RPMI 1640(2) without L-Glutamine without Calciumwith 2.0 g/L NaHCO3 500 ml P04-16151

RPMI 1640(2) without L-Glutaminewithout L-Tryptophanwith 2.0 g/L NaHCO3 500 ml P04-17599

RPMI 1640(2)

without L-Glutaminewithout Glucosewith 2.0 g/L NaHCO3 500 ml P04-17550

RPMI 1640(2)

without L-Glutaminewith 15 mM Hepeswithout Phosphatewith 2.0 g/L NaHCO3 500 ml P04-21049

RPMI 1640 (10X)(2)


RPMI 1640(2)

without L-Glutaminewith 25 mM Hepeswithout NaHCO3 500 ml P04-17850

RPMI 1640(2) without L-Glutaminewith 25 mM Hepeswith 2.2 g/L NaHCO3 500 ml P04-22500

RPMI 1640(2)


Powder Media without Glutamine

RPMI 1640(1) without L-Glutamine 10 L P03-7210without NaHCO3 50 L P03-7250

RPMI 1640(1)

without L-Glutamine without Phenol red 10 L P03-7710without NaHCO3 50 L P03-7750

RPMI 1640(1)

without L-Glutaminewith 25 mM Hepes 10 L P03-4410without NaHCO3 50 L P03-4450


Calcium nitrate x 4H2OPotassium chlorideMagnesium sulfate anhydrousSodiumchloridedi-Sodium hydrogen phosphate

100.00400.00

48.836000.00


D(+)-Glucose anhydrousGlutathion (red.)HepesPhenol red

2000.001.00

5958.005.00

AminoAcids

L-Arginine x HClL-Asparagine x H2OL-Aspartic acidL-Cystine x 2HClL-GlutamineL-Glutamic acidGlycineL-Histidine x HCl x H2OL-HydroxyprolineL-IsoleucineL-LeucineL-Lysine x HClL-MethionineL-PhenylalanineL-ProlineL-SerineL-ThreonineL-TryptophanL-Tyrosine x 2NaL-Valine

241.8650.0020.0065.19

300.0020.0010.0020.2720.0050.0050.0040.0015.0015.0020.0030.0020.00

5.0028.8320.00

Vitamins p-Aminobenzoic acidD-(+)-BiotinD-Calcium pantothenateCholine chlorideFolic acidmyo-InositolNicotinamidePyridoxine x HClRiboflavinThiamine x HClVitamin B12

1.000.200.253.001.00

35.001.001.000.201.00

0.005

3 3


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Media

Page 68

RPMI 1640


MediaMedia

Schneider´s Drosophila MediumDescriptionOriginally developed for the culture of Drosophila cells, this medium is also suitable for the culture of other dipteran cell lines.

Composition

Liquid Media

Schneider’s Drosophila Medium(1)




Powder Media




Potassium chloridePotassium dihydrogen phosphateMagnesium sulfate driedSodium chloridedi-Sodium hydrogen phosphate

1600.00450.00

2585.712100.00


DL-Malic acidSuccinic acidFumaric acidD(+)-Glucose anhydrousYeast extractα-Ketoglutaric acid sodium saltD(+)-Trehalose x 2H2O

600.0060.0060.00

2000.002000.00

402.662210.00

AminoAcids

β-AlanineL-Arginine BaseL-Asparatic acidL-Cysteine free baseL-CystineL-GlutamineL-Glutamic acidGlycineL-Histidine BaseL-IsoleucineL-LeucineL-Lysine x HClL-MethionineL-ProlineL-SerineL-ThreonineL-TryptophanL-TyrosineL-Valine

500.00600.00400.00

60.0016.60

1800.00800.00250.00400.00150.00150.00

1650.00150.00

1700.00250.00350.00100.00500.00300.00

Liquid Media with L-Glutamine

RPMI 1640(1)


RPMI 1640(1) with L-Glutaminewithout Phenol redwith 2.0 g/L NaHCO3 500 ml P04-16515

RPMI 1640(1)

with 2 mM L-Glutaminewith 1 mM Sodium pyruvatewith 4.5 g/L Glucosewith 10 mM Hepeswith 1.5 g/L NaHCO3 500 ml P04-18047

RPMI 1640(1) with L-Glutaminewith 25 mM Hepeswith 2.2 g/L NaHCO3 500 ml P04-22100

Special Media with L-Glutamine

RPMI 1640(2) with L-Glutaminewithout Glucosewith 2.0 g/L NaHCO3 500 ml P04-17545

RPMI 1640(2) with L-Glutaminewithout L-Argininewith 2.0 g/L NaHCO3 500 ml P04-16598

RPMI 1640(2)

with L-Glutaminewithout L-Tryptophanwith 2.0 g/L NaHCO3 500 ml P04-17598

RPMI 1640(2) with L-Glutaminewith 20 mM Hepeswith 0.85 g/L NaHCO3 500 ml P04-19550

Liquid Media with stab. Glutamine

RPMI 1640(1)


RPMI 1640(1) with stab. Glutaminewith 25 mM Hepeswith 2.2 g/L NaHCO3 500 ml P04-18050

Special Media with stab. Glutamine

RPMI 1640(2) with stab. L-Glutaminewithout Phenol redwith 2.0 g/L NaHCO3 500 ml P04-16520

RPMI 1640(2) with stab. Glutaminewithout Glucosewith 2.0 g/L NaHCO3 500 ml P04-17546

RPMI 1640(2) with stab. Glutaminewithout Phenol redwithout Glucosewith 2.0 g/L NaHCO3 500 ml P04-16530

3 3


Powder Media with L-Glutamine

RPMI 1640(1) with L-Glutamine 10 L P03-4310without NaHCO3 50 L P03-4350

RPMI 1640(1)


RPMI 1640(1)

with L-Glutaminewithout Phenol red 10 L P03-7610without NaHCO3 50 L P03-7650

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Media


MediaMedia

TC 100 Insect MediumDescriptionThe TC 100 Insect Medium is an absolutely serum-free formula (Oxford formulation) for the growth of insect cells, especially for SF9 cells and the breeding of viruses. If you would like to work with a modern protein-free insect medium, our Spodopan is the ideal choice.

Composition

Liquid Media

TC 100 Insect Medium(1)


Special Media



Powder Media




Calcium chloride x 2H2OPotassium chlorideMagnesium chloride x 6H2OMagnesium sulfate driedSodium dihydrogen phosphate x H2O

1298.132900.002282.591781.00

970.00

OtherCompo-nents

D(+)-Glucose anhydrousBacto - Tryptose

1000.002600.00

AminoAcids

L-AlanineL-Arginine BaseL-Aspartic acidL-Asparagine x H2OL-CystineL-GlutamineL-Glutamic acidGlycineL-Histidine x HCl x H2OL-IsoleucineL-LeucineL-Lysine x HClL-MethionineL-PhenylalanineL-ProlineL-SerineL-ThreonineL-TryptophanL-TyrosineL-Valine

225.00550.00350.00391.97

20.00600.00600.00650.00

3400.0050.0075.00

630.0050.00

150.00350.00550.00180.00100.00

55.00100.00

Vitamins p–Amino benzoic acidD-(+)-BiotinD-Calcium pantothenateFolic acidmyo-InositolNicotinic acidPyridoxine x HClRiboflavinThiamine x HClVitamin B12

0.020.010.110.020.020.020.020.020.020.01

TNM-FH MediumDescriptionThe TNM-FH is a variation of the Grace medium. This modification has proved as a good culture medium for many lepidopteran cells.

Liquid Media

TNM-FH Medium(1)

with L-Glutaminewith Lactalbumine-Hydrolysatewith Yeast extractwith 0.35 g/L NaHC03 500 ml P04-80500

Special Media

TNM-FH Medium(2)

with L-Glutaminewith Lactalbumin-Hydrolysatewith Yeast extractwith 10 % Fetal Bovine Serumwith 0.35 g/L NaHCO3 500 ml P04-83500

Powder Media

TNM-FH Insect Medium(1)

without L-Glutaminewith Lactalbumine-Hydrolysatewith Yeast extract 10 L P03-9710without NaHCO3 50 L P03-9750

Components mg/L

Inorganic Salts

Calcium chloride x 2H2OPotassium chlorideMagnesium chloride x 6H2OMagnesium sulfate drieddi-Sodium hydrogen phosphate

1,324.622,240.002,278.861,939.80

876.92

OtherCompo-nents

DL-Malic acidSuccinic acidD-FructoseFumaric acidD(+)-Glucose anhydrousYeast extractα-Ketoglutaric acid sodium saltLactalbumin HydrolysateSucrose

670.0060.00

400.0055.00

700.003,333.33

425.66

3,333.3326,680.00

AminoAcids

β-AlanineL-AlanineL-Arginine x HClL-Asparagine x H2OL-Aspartic acidL-CystineL-GlutamineL-Glutamic acidGlycineL-Histidine BaseL-IsoleucineL-LeucineL-Lysine x HClL-MethionineL-PhenylalanineL-ProlineL-SerineL-ThreonineL-TryptophanL-TyrosineL-Valine

200.00225.00700.00350.00350.00

19.18600.00600.00650.00

2,500.0050.0075.00

625.0050.00

150.00350.00550.00175.00100.00

50.00100.00

Vitamins p-Aminobenzoic acidD-(+)-BiotinD-Ca-PantothenateCholin chlorideFolic acidmyo-InositolNicotinic acidPyridoxol x HClRiboflavinThiamine x HCl

0.020.010.020.200.020.020.020.020.020.02

Composition

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Media


MediaMedia

Waymouth´s MB 752/1 MediumDescriptionWaymouth‘s MB 752/1 Medium was developed for studies concerning nutrition and metabolism. It also can be used for growing strain L sub-lines, NCTC clone 929.

Composition


Special Media

Waymouth’s MB 752/1 Medium(2)


Powder Media

Waymouth’s MB 752/1 Medium(1)


Components mg/L

Inorganic Salts

Calcium chloride x 2H2OMagnesium chloride x 6H2OMagnesium sulfate driedPotassium chloridePotassium dihydrogen phosphateSodium chloridedi-Sodium hydrogen phosphate anhydrous

120.02240.00130.96150.00

80.00

6,000.00300.00

OtherCompo-nents

D(+)-Glucose anhydrousGlutathione (red.)HepesHypoxanthinePhenol red

5,000.0015.00

4,766.4025.0010.00

AminoAcids

L-Arginine x HClL-Aspartic acidL-Cysteine x HCl x H2OL-CystineL-GlutamineL-Glutamic acidGlycineL-Histidine x HCl x H2OL-IsoleucineL-LeucineL-Lysine x HClL-MethionineL-PhenylalanineL-ProlineL-ThreonineL-TryptophanL-TyrosineL-Valine

75.0060.00

100.2615.00

350.00150.00

50.00164.10

25.0050.00

240.0050.0050.0050.0075.0040.0040.0065.00

Vitamins L-Ascorbic acidD(+)-BiotinD-Calcium pantothenateCholine chlorideFolic acidmyo-InositolNicotinamidePyridoxine x HClRiboflavinThiamine x HClVitamin B12

17.500.021.00

250.000.401.001.001.001.00

10.000.20

Composition


Components mg/L

Inorganic Salts

Calcium chloride x 2H2OIron(III)-nitrat x 9H2OPotassium chlorideCopper(II)-sulfate x 5H2OMagnesium sulfate driedManganese chloride x 4H2OSodium chlorideSodium dihydrogen phospha-te x H2OZinc sulfate x 7H2O

264.920.0001400.000.0001139.570.0001

6,800.00140.00


D(+)-Glucose anhydrousHepesGlutathion (red.)MethyllinoleatSodium pyruvatePhenol red

2,000.005,958.00

0.050.03

25.0010.00

AminoAcids

L-AlanineL-Arginine free baseL-Asparagine x H2OL-Aspartic acidL-CysteineL-CystineL-GlutamineL-Glutamic acidGlycineL-Histidine BaseL-IsoleucineL-LeucineL-Lysine x HClL-MethionineL-PhenylalanineL-ProlineL-SerineL-ThreonineL-TryptophanL-TyrosineL-Valine

90.0050.0020.0030.0040.0020.00

292.0050.0050.0015.0050.0075.0087.5015.0025.0030.0010.0040.0010.0035.0050.00

Vitamins L-Ascorbic acidD(+)-BiotinCalciferolD-Calcium pantothenateCholine chlorideFolic acidmyo-InositolMenadion sodium bisulfiteNikotinamidPyridoxal x HClRiboflavinThiamine x HClDL-α-Tocopherol phospha-te-Na2Vitamin A acetateVitamin B12

2.000.500.101.001.501.002.000.011.001.000.101.000.01

0.100.20

William´s Medium EDescriptionThe William‘s Medium E is used for long-term cultivation of adult rat liver epithelial cells.

Liquid Media

William’s Medium E(1)








Special Media

William´s Medium E(2)

without L-Glutaminewithout Glucosewith 2.24 g/L NaHCO3 500 ml P04-29050S1

Powder Media



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Media

Page 74

Endopan 3Large Vessel Endothelial Cell Medium

BackgroundEndothelial cells line the blood and lymphatic vessels and the internal cavities of the heart. They display a strongly flattened, polygonal form and mostly rest on a basal membrane. They adhere to each other by desmosomes and tight-junctions.

With a total cell number of about one trillion (1012), the endothelium is one of the biggest organs of the body and plays a key role in many physiological and patho-physiological processes (e.g. cell-based immune response, wound healing, inflammation, allergy, cardiovascular diseases, tumour growth). A huge number of soluble factors circulating in the blood or released by neighbouring cells, control proliferation or apoptosis of endothelial cells and the invasion and migration of leucocytes to the endothelium, thereby regulating the maintenance, degeneration, or regeneration of blood vessels.

The endothelium constitutes a highly specialized organ that lines the vascular system and lymphatic channels in a complex network of arteries, veins, and micro-vessels which differ in size, structure, and function. The cultivation of endothelial cells from large vessels, predominantly from human umbilical vein, is a routine procedure in many laboratories, and this has contributed huge to the development of modern vascular biology. However, there is convincing evidence that micro-vascular endothelial cells display a number of important functional differences, compared to large vessel-derived

CompositionEndopan 3 ready-to-use is a specially developed medium for the in vitro culture of human endothelial cells containing all components necessary for optimal growth. It is designed for use in an incubator at 37° C with a 5% CO2 atmosphere.

Endopan 3 ready-to-use(1) 500 ml P04-00100Endopan 3 kit with 9 supplements(1) 500 ml P04-0010K

Endopan MVMicrovascular Endothelial Cell Medium

Endopan MV ready-to-use(3) 500 ml P04-00200Endopan MV kit with 8 supplements(3) 500 ml P04-0020K


MediaMedia

Endopan PROEndothelial Progenitor Medium

BackgroundEndothelial cells line blood and lymphatic vessels and the internal cavities of the heart. They display a strongly flattened, polygonal form and mostly rest on a basal membrane. With a total number of about 1012 cells, the endothelium is one of the biggest organs of the body and plays a key role in many physiological and patho-physiological processes. A number of factors control proliferation or apoptosis of endothelial cells, thereby regulating the maintenance, degeneration, or regeneration of blood vessels.

New blood vessel formation occurs via angiogenesis or vasculogenesis, a process thought to be restricted to embryonic development. In 1997, postnatal vasculogenesis has been proposed as an important mechanism for angiogenesis via blood or bone marrow derived circulating progenitor endothelial cells (PEC) (Asahara et al, Science 1997). Consequently, PECs have been extensively studied as a potential cell therapy for the repair of damaged blood vessels. Animal studies clearly demonstrated that administration of PECs partially rescued cardiovascular dysfunction or myocardial injury with evidence for PEC contribution to new vessel growth.In most studies, PECs are defined by cell surface expression of CD34, CD133, or VEGF-R2 (KDR). Because these molecules are also present on hematopoietic progenitors, relying only on surface markers can not exclude a contamination with hematopoietic linage cells. More recently, a PEC population has been identified which shows expression of endothelial as well as progenitor, but not hematopoietic cell markers (Ingram et al, Blood. 2004;104:2752). Importantly, these cells have been tested for a high proliferative potential in clonogenic assays and additionally characterized by formation of functional blood vessels in vivo (Yoder et al, Blood. 2007;109:1801).

CompositionEndopan PRO ready-to-use is a complete medium specially developed for the in vitro culture of human progenitor endothelial cells (hPEC) containing all components necessary for optimal colony formation, clonogenic growth, and rapid proliferation.

Endopan PRO kit is provided with FBS growth supplement (pre-screened and tested for progenitor cells) and additional supplements in separate sterile packing. This will enable the user to prepare a medium for special application.

Endopan PRO ready-to-use(3) 500 ml P04-00700Endopan PRO kit with 6 supplements(3) 500 ml P04-0070K

endothelial cells, with regard to their growth factor response and their regulation of adhesion molecule expression.

They serve as the barrier separating circulating blood from the extracellular matrix and interstitium in the body. Cells involved in the pathogenesis of tumor angiogenesis, wound healing, and acute or chronic inflammation are predominantly of micro-vascular origin. Several functions associated with the micro-vasculature in situ are expressed by micro-vascular endothelial cells in cell culture.

Micro-vessels are not simply tubes but have also a second cellular component, the mural cell or pericyte. Little is known about later stages of vessel growth, including the addition of pericytes to the capillary and its influence on endothelial growth and function. In vivo, pericytes form an incomplete envelopment around the endothelial cells within the micro-vascular basement membrane of capillaries and post-capillary venules.

Evidence clearly indicates that differences exist between endothelial cells of the microvasculature and those that line large vessels. These include differences in secreted products, in the expression of cell adhesion molecules, and in cytokine-induced regulation of cell adhesion molecules. Thus, a precise delineation of the biology of micro-vascular endothelial cells is crucial to our understanding of such important processes as inflammation, tumor progression, cardiac microcirculation, and blood-brain barrier function.

Endopan

Endopan 3 kit is provided with FBS and supplements in separate sterile packing. This will enable the user to prepare a medium for special application. For example, FBS, VEGF, FGF-2, or other components may be omitted from the complete medium for specific experimental settings.

hPEC colony (P1) with outgrowing cells in Endopan PRO

hPEC in Endopan PRO (P6)

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Media

Page 76

HepatopanHuman Hepatocyte Medium

DescriptiomLike every human organ, the liver consists of a complicated compound of different cells with varying functions. Hepatocytes represent – in terms of figures – with 75 % of the total number of liver cells the most important component. The metabolism, this means the chemical transformation of almost all substances which are taken in by the body, takes place in the liver.

CompositionThe hepatocyte medium from PAN-Biotech is supplied as a basal medium with four supplements (storage of the supplements at -20° C). The supplements have to be ad-ded to the medium before use. The medium does not con-tain fetal bovine serum.

Hepatopan with 4 supplements(3) 500 ml P04-00600


MediaMedia

MelanopanMelanocyte Medium

DescriptionMelanocytes are embedded in the basal and spike cell lay-er of the epidermis. They produce the pigment melanin and take care of the protective function of the skin against UV damages. If the solar radiation is too strong, the me-lanocytes are damaged and can develop into tumour cells.

CompositionThe melanocyte growth medium from PAN-Biotech is supplied as a basal medium with seven supplements (storage of the supplements at -20° C). The supplements have to be added to the medium before use. The medium does not contain fetal bovine serum.

Melanopan with 7 supplements(3) 500 ml P04-740500

StempanES-Cell Medium

DescriptionStem cells are non-specialized cells with the ability (potency) to develop into different organo-typic cell types (e. g. heart, nerve, blood, muscle and cartilage cells). Depending on their origin, they are divided into embryonic and adult stem cells.

CompositionFor the cultivation of embryonic stem cells, PAN-Biotech has developed a complete ready-to-use medium. The medium contains fetal bovine serum.

Stempan DMEM(2)

with L-Glutaminewith 3.7 g/L NaHCO3without LIF

500 ml P08-50500

Stempan GMEM(2)

with L-Glutaminewith 2.75 g/L NaHCO3without LIF

500 ml P08-50600

EMEM FibroblastsFibroblast Medium

DescriptionBased on EMEM, this medium was supplemented with amino acids and vitamins and optimized for an improved growth of fibroblasts.

EMEM Fibroblasts(1) 500 ml P04-08049

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Neuropan-Basal Media (Basicmedia)(2) 500 ml P04-00900

Neuropan 27 supplement 20x(2) 100 ml10 ml

P07-07100P07-07010


P07-07200P07-07210

Neuropan 27 supplement 20xwithout Antioxidant(2)

100 ml10 ml

P07-10100P07-10010


P07-11100P07-11010

NS21 Supplement 50x sterile(3) 10 ml P07-20021NS21 Supplement 50x non-sterile(3) 10 ml P07-20001

NeuropanNeuronal Cell Medium

Neuropan 27 is a concentrate for the serum-free cultivation of neural cells.

NS21 SupplementTo culture neurons in the absence of serum, defined supplements such as B27 are widely used. However, available supplements exhibit some variability in their capability to support neurons in culture. NS21 Supplement is a newly developed serum substitute for neuronal cultures of cells from the central and peripheral nervous system.

Neuropan Basal MediumNeuropan basal medium supports the growth of hippocampus cells and many other neuronal cells of the central nervous system. A feeder layer of astrocytes is not required. Neuropan basal medium does not contain glutamate which should be added for the initial culture (25 µM). Before use, Neuropan basal medium is supplemented with serum or for a serum-free culture with Neuropan 27 or NS21 Supplement.

Pantum

Pantum 386(3) 500 ml P04-00386

Pantum 586A(3) 500 ml P04-00586

Pantum L24(3) 500 ml P04-00024

Pantum T64(3) 500 ml P04-00064

DescriptionPantum are ready-to-use growth media which contain purified plasma proteins and lipids, such as serum albumin and cholesterol, specific growth factors, components of soybean extract, an iron transport protein and enriched trace elements. The new formulations result in stable cell growth under defined culture conditions. No addition of serum or growth factors is necessary.

Pantum 386 for epithelial cellsPantum 386 is a modified formulation of DMEM and specially developed to optimize the growth of epithelial cell lines.

Pantum 586A for adherent cellsPantum 586A is particularly suited for culture of adherent cells to stimulate their growth. It is a modification of Iscove’s MEM.

Pantum L24 for lymphocytesPantum L24 is suited for culture of peripheral blood lymphocytes. Adult lymphocytes lack the ability to proliferate. Therefore, Pantum L24 contains a mitogen (phytohemagglutinin, PHA) specifically acting on the cell cycle. It is a modified formulation of RPMI 1640.

Pantum T64 for tumor cellsPantum T64 is specially developed for culture of tumor cells to stimulate the growth of this cell type. It is a modification of RPMI 1640.

For the cultivation of fibroblasts, this medium has to be supplemented with 10% FBS before use.

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Media

Page 78

AmniopanPrenatal Cytogenetics Medium

DescriptionAmniopan is a complete ready-to-use medium intended for in vitro diagnostic use with a short term culture of human fetal cells from amniotic fluid or chorion villi biopsy (CVS) material for a standardized application in cytogenetic studies.Amniopan is intended for in vitro use and has been de-signed for establishing cultures of human fetal cells from amniotic fluid or chorion villi biopsies (CVS), which then can be used in karyotyping, fluorescence in-situ hybridisation (FISH) or other cytogenetic procedures.The media formulation of Amniopan was optimized on human fetal cells from amniotic fluid and CVS, with special emphasis on fast attachment of cells to the cell culture substrate and efficient cell growth to facilitate rapid diagnostic findings.

CompositionAmniopan is supplied frozen as a complete medium, ready-to-use in a 100 ml format. It is based on al-pha-MEM and contains antibiotics, L-glutamine, foetal bovine serum (FBS), hormones and growth factors.

SuitabilityAmniopan is a complete medium (ready-to-use) for the cultivation of human fetal cells from amniotic fluid and chorion villi biopsy samples. It is suitable for a rapid ex-pansion of amniotic cells in order to investigate chromo-somal disorders. The number and quality of metaphases in Amniopan are significantly higher and independent of individual batches as compared to other media.

Amniopan(1) 100 ml P04-70100

Amniopan S2Prenatal Cytogenetics Medium

DescriptionAmniopan S2 is a complete ready-to-use medium inten-ded for in vitro diagnostic use with a short term culture of human fetal cells from amniotic fluid or chorion villi biopsy (CVS) material for a standardized application in cytogenetic studies.Amniopan S2 is intended for in vitro use and has been designed for establishing cultures of human fetal cells from amniotic fluid or chorion villi biopsies (CVS), which then can be used in karyotyping, fluorescence in-situ hybridisation (FISH) or other cytogenetic proce-dures. The media formulation of Amniopan S2 was further optimized on human fetal cells from amniotic fluid and CVS, with special emphasis on fast attachment of cells to the cell culture substrate and efficient cell growth to facilitate rapid diagnostic findings.

CompositionAmniopan S2 is supplied frozen as a complete medi-um, ready-to-use in a 100 ml format. It is based on alpha-MEM and contains antibiotics, L-glutamine, fetal bovine serum (FBS), hormones and an increased amount of growth factors.

SuitabilityAmniopan S2 is a ready-to-use medium for the cultivati-on of human fetal cells from amniotic fluid and chorion villi biopsy samples. It is suitable for a rapid expansion of amniotic cells in order to investigate chromosomal disorders. The number and quality of metaphases in Amniopan S2 are significantly higher and independent of individual batches as compared to other media.

Amniopan S2(1) 100 ml P04-70101


MediaMedia

MarrowpanMarrow Cell Medium

DescriptionMarrowpan is a complete ready-to-use medium inten-ded for in vitro diagnostic procedures with a short term culture of bone marrow and other hematopoietic cells for cytogenetic studies.Marrowpan is intended for in vitro use and has been designed for establishing cultures of bone marrow and leukemic blood cells, which then can be used in karyoty-ping, fluorescence in-situ hybridisation (FISH) or other cytogenetic procedures.Marrowpan can be used as a neutral medium to culture different haematopoietic cells (myeloid and lymphoid lineages) present in bone marrow or leukemic blood samples. Marrowpan is also used together with a mito-gen specific for B or T lymphocytes where these particu-lar lineages are being investigated.

CompositionMarrowpan is supplied frozen as a complete medium, ready-to-use in a 100 ml format. It is based on al-pha-MEM and contains antibiotics, L-glutamine, fetal bovine serum (FBS), hormones and growth factors.

SuitabilityMarrowpan is a complete medium (ready-to-use) for the cultivation of cells from peripheral blood or bone marrow. It is suitable for a rapid expansion of blood cells in order to investigate leukemic diseases (e.g. ALL, AML, CLL, CML, MPN, MDS). The number and quality of metaphases in Marrowpan are significantly higher and independent of individual batches as compared to serum-containing media.

Marrowpan(1) 100 ml P04-70200

Marrowpan S2Marrow Cell Medium

DescriptionMarrowpan S2 is a complete ready-to-use medium intended for in vitro diagnostic procedures with a short term culture of bone marrow and other hematopoietic cells for cytogenetic studies. Marrowpan S2 is intended for in vitro use and has been designed for establishing cultures of bone marrow and leukemic blood cells, which then can be used in karyotyping, fluorescence in-situ hybridisation (FISH) or other cytogenetic proce-dures.Marrowpan S2 can be used as a neutral medium to culture different haematopoietic cells (myeloid and lymphoid lineages) present in bone marrow or leukemic blood samples. Marrowpan S2 is also used together with a mitogen specific for B or T lymphocytes where these particular lineages are being investigated.

CompositionMarrowpan S2 is supplied frozen as a complete me-dium, ready-to-use in a 100 ml format. It is based on alpha-MEM and contains antibiotics, L-glutamine, Foetal Bovine Serum (FBS), hormones and an increased amount of growth factors.

SuitabilityMarrowpan S2 is a ready-to-use medium for the culti-vation of cells from peripheral blood or bone marrow. It is suitable for a rapid expansion of blood cells in order to investigate leukemic diseases (e.g. ALL, AML, CLL, CML, MPN, MDS). The number and quality of metaphases in Marrowpan S2 are significantly higher and independent of individual batches as compared to serum-containing media.

Marrowpan S2(1) 100 ml P04-70201

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Index


Index

Alphabetical Product Index Alphabetical Product Index

Product PageAAccutaseAgaroseAlbumin, bovineAlbumin, humanAlpha MEMAmino AcidsAmniopanAmniopan S2Amphotericin BAnimal SerumAntibioticsAntifungal DrugsAttachment Factors

100131104104

3897767698119898

102

BBacitracin BBarrycidal BarrydinBiologicalsBME SolutionBME VitaminsBME with Earle´s SaltsBME with Hank´s SaltsBovine SerumBovine Serum Albuminβ-Mercapthoethanol

98110111115

97974040

5104

93

CChemokinesCMRL-1066 MediumCollagen ACollagen RCollagenaseCryo PreservationCryopan

12041

102102100108108

DDemecolcin SolutionDesipureDimethylsulfoxideDisinfectantsDispaseDMEM/F12 MediumDMSOdNTP MixdNTP SetsDulbecco´s Modified Eagle MediumDulbecco´s Phosphate Buffered Salt Solution

93112108109101

46108133132

42

94

Product PageEEarl´s Buffered Salt SolutionEDTAEMEM Fibroblast MediumEndopan 3Endopan 300 SLEndopan MVEndopan PROEnzymes for Cell Dissociation

94101

7572347273

100

FFBSFBS BiotechFBS GoodFibronectinFreezing Medium

565

103108

GGelatine SolutionGentamycin SulphateGrey´s Balanced Salt SolutionGlasgow MEM (BHK 21)Grace´s Insect MediumGrowth Factors

10298964849

116

HHam´s F10 MediumHam´s F12 MediumHank´s Balanced Salt SolutionHAT SupplementHT SupplementHepatopanHepes - Sodium SaltHepes BufferHuman CytokinesHuman SerumHuman Serum AlbuminHuman Transferrin apoHygromycin B

5051959393749393

11612

1049398

IInsulin HumanIPL-41 Insect MediumIscove´s Modified Dulbecco´s MediumITS Solution

935452

93

JJoklik-MEM 55

KKanamycin Sulphate 98

Product PageLLaminin MouseLeibovitz´s L-15 MediumL-Glutamine

1035697

MMarrowpanMarrowpan S2Mc Coy´s 5A MediumMCDB MediumMedia SupplementsMedium 199 with Earle´s SaltsMedium 199 with Hank´s SaltsMelanopanMEMMEM Vitamine SolutionMEM with Earle´s SaltsMEM with Hank´s SaltsMinocyclinMolecular Biology Mycorase

77775758935960749797616398

12399

NNeomycin SulphateNeuropanNystatin Solution

987598

PPAN DNA CleanPAN Hot Start DNA-PolymerasePAN Ladder IPAN SL-S Product LinePancollPanserin T3Paneticin 420Panexin BMMPanexin NTAPanexin NTSPanexin SL-SPANScript DNA-PolymerasePANScript Red DNA-PolymerasePansera ESPanserin 293APanserin 293SPanserin 401Panserin 411Panserin 411SPanserin 412Panserin 413Panserin 416Panserin 701Panserin 801

130126129107105

2899201819

107124125

727272122222323232929

Product PagePPanserin C6000Panserin H4000Panserin H8000Panserin ProVeroPanserin PX10Panserin PX40Panserin S2Panserin T3PANsys 3000PantumPenicillinPluronicPolymerasesPolymycin BPowerScript DNA-Polymerase Long RangePowerScript DNA-Polymerase Short RangePowerStem ESPro 1PowerStem ESPro2PowerStem ESTPowerStem HE1PowerStem HE2PowerStem HPSCPowerStem iPS1PowerStem iPS2PowerStem MSC1PowerStem PEC1 PowerStem PEC1 kitProteinase KPuck´s Salt Solution A

2624252830313328

135749993

12399

128

127

8182838485898687889090

12996

RRPMI 1640 64

SSchneider´s Drosophila MediumSeparating SolutionsSeparating Solutions Pre-FilledSL-S CollagenSL-S CryopanSL-S MediumSL-S TrypsinSodium BicarbonateSodium ChlorideSodium PyruvateSpodopanStempanSterile WaterStreptomycin

67105106107107107107

93939332759399


Index


Index

Alphabetical Product Index

Product PageTTC 100 Insect MediumTiamulinTNM-FH MediumTreated SerumTryode´s Salt SolutionTrypsinTryptose Phosphate

6899691096

10193

VVitamins 97

WWaymouth´s MB 752/1 MediumWilliam´s Medium E

7071

ZZeocin 99

Numeric Product Index

Product No. Page Product No. Page Product No. Page Product No. Page36000036005036010136040036100036500046500066000070050070100071050072050072050173001074001027050052705001SCB-11000050CB-1101001CB-1101002CB-1101003CB-1102010CB-1102011CB-1102021CB-1102023CB-1102024CB-1104112CB-1104113CB-1104201CB-1104202CB-1105001CB-1106001CB-1108002CB-1108003CB-1108010CB-1108011CB-1108100CB-1109200CB-1109201CB-1109301CB-1110000CB-1110001CB-1110002CB-1111122CB-1111123CB-1111131CB-1111151CB-1111153CB-1111162

109109109109109109109109109109109109109109109103103119116116116116116116116116116116116116117117116116116116116118118118118118118118118118118118118

CB-1112011CB-1112012CB-1113008CB-1113011CB-1113012CB-1114002CB-1114011CB-1114013CB-1114100CB-1114102CB-1115000CB-1115001CB-1115002CB-1115006CB-1116001CB-1117001CB-1117001MCB-1117007CB-1117008CB-1118005CB-1118008CB-1119000CB-1119001CB-1122001CB-1125032CB-1127000CB-1127100CB-1200000CB-1210000CB-1212011 CB-1212011MCB-1214000CB-1214001CB-1214120CB-1214121CB-1232701CB-1300058CB-1300118CB-1407003CB-1412011CB-1515001CB-2015001CB-2110000CB-2110002CB-2110003CB-2110005CB-2110100CB-2110101CB-2120100

118118116116116118118118118118116116116116116117117119119120120116116120117118118119119119119119119119119120117118120119116116116116116116116116117

CB-2120101CB-2120110CB-2120112CB-2120121CB-2130120CB-2130121CB-2130123CB-2130202CB-2130203CB-2130300CB-2130301CB-2130405CB-2130407CB-2130501CB-2130600CB-2130603CB-2131000CB-2131001CB-2131201CB-2131500CB-2131501CB-2210000CB-2210001CB-2210002CB-2230030CB-2230031CB-2230111CB-2230120CB-2230121CB-2230220CB-2230221CB-2230300CB-2230301CB-2230302CB-2230403CB-2230600CB-2230601CB-2230602CB-2231202CB-2232000CB-2232002CB-2250001CB-2310015CB-2310016CB-2330000CB-2331001CB-2420031CB-2420032CB-2430122

117117117117117117117117117117117117117117117117117117117117117119119119119119119119119119119119119119119119119119119120120119119119119119119119119

CB-2800001CB-2800002CB-2800007CB-2800008CB-2800010CB-2800011CB-3410010CB-3410011CB-4070010LS0002100LS0004174LS0004176LS0004180LS0004182LS0004186LS0004188LS0004194LS0004196MB-1100500MB-1100600MB-1120250MB-1120500MB-1860250MB-1860500MB-1865000MB-30010250MB-30020250MB-4300002P03-0010P03-0050P03-01510P03-01550P03-02010PP03-02050PP03-0510P03-0550P03-0710P03-0750P03-0810P03-0850P03-0910P03-0950P03-1010P03-1050P03-1110P03-1150P03-1210P03-1250P03-1310

116116116116116116118118117101100100100100100100100100124125128128126126126123123129

424242425555424245454545454545454747474753


Index


Index


Product No. Page Product No. Page Product No. Page Product No. PageP03-1350P03-1410P03-1450P03-1510P03-1550P03-1610P03-1650P03-1710P03-1750P03-1810P03-1850P03-1910P03-1950P03-2110P03-2150P03-2310P03-2350P03-2410P03-2450P03-2510P03-2550P03-2610P03-2650P03-2710P03-2750P03-2810P03-2850P03-2910P03-2950P03-3010P03-3050P03-3110P03-3150P03-3310P03-3350P03-3910P03-3950P03-4010P03-4050P03-4110P03-4150P03-4310P03-4350P03-4410P03-4450P03-4510P03-4550P03-4810P03-4850

53535356565656575757575959606039393939393939396262626262626262484863635050505051516666656570707171

P03-5010P03-5050P03-5210P03-5250P03-6010P03-6050P03-6110P03-6150P03-6510P03-6550P03-6810P03-6850P03-6910P03-6950P03-7210P03-7250P03-7310P03-7350P03-7410P03-7450P03-7610P03-7650P03-7710P03-7750P03-9010P03-9050P03-9110P03-9150P03-9210P03-9250P03-9310P03-9350P03-9610P03-9650P03-9710P03-9750P04-00024P04-00064P04-00100P04-0010KP04-00200P04-0020KP04-00386P04-00507P04-00508P04-00509P04-00586P04-00600P04-00650

50505353474747474545484848486565666661616666656549494949545467676868696974747272727274616262747434

P04-0065KP04-00700P04-0070KP04-00900P04-01158P04-01159P04-01161P04-01163P04-01500P04-01501P04-01515P04-01516P04-01548 P04-01548S1P04-01549P04-01550P04-01551P04-01555P04-01597P04-02500P04-02500S1P04-02501P04-03500P04-03503P04-03510P04-03550P04-03551P04-03556P04-03560P04-03588P04-03590P04-03591P04-03596P04-03598P04-03600P04-04057P04-04500P04-04510P04-04550P04-05050P04-05500P04-05540P04-05545P04-05550P04-05551P04-05610P04-06500P04-07050P04-07150

34737375434343444243424342424242424344424343434444434443444443434344434443434457574444444257575959

P04-07250P04-07450P04-07500P04-07600P04-07753P04-08049P04-08050P04-08056P04-08150P04-08250P04-08500P04-08509P04-08510P04-08549P04-09050P04-09500P04-10050P04-10500P04-10599P04-12049P04-12050P04-12500P04-13050P04-13500P04-14500P04-14501P04-14550P04-14559P04-15500P04-15600P04-16151P04-16500P04-16515P04-16516P04-16520P04-16530P04-16598P04-17500P04-17510P04-17545P04-17546P04-17550P04-17598P04-17599P04-17850P04-18000P04-18047P04-18050P04-18500

59605960607561626162626162626162636363505050505051515151515165666664666666646566666566656564666666


Product No. Page Product No. Page Product No. Page Product No. PageP04-19500P04-19550P04-20050P04-20150P04-20150S2P04-20250P04-20259P04-20350P04-20351P04-20450P04-20451P04-20451S1P04-21049P04-21050P04-21051P04-21060P04-21150P04-21200P04-21250P04-21300P04-21350P04-21500P04-21502P04-22100P04-22500P04-25050P04-25500P04-26050P04-27050P04-27054P04-27055P04-27500P04-28500P04-29050P04-29050S1P04-29150P04-29500P04-29510P04-30010PP04-300500P04-30050PP04-30500P04-31500P04-32010PP04-32050PP04-32100P04-32105P04-32500P04-32505

656652525352535253535353653838383855385538383866654040405656565670717171717194

107949494959595959595

P04-33010PP04-33050PP04-33100P04-33500P04-34100P04-341000P04-34500P04-35500P04-360010BP04-36050PP04-361000P04-362500P04-3625CP04-36500P04-3650CP04-37500P04-38500P04-39500P04-41150P04-41151P04-41250P04-41251P04-41252P04-41450P04-41500P04-41550P04-41650P04-43100P04-44100P04-47500P04-48010PP04-48050PP04-48500P04-49100P04-49105P04-49500P04-49505P04-50100P04-50105P04-50500P04-50505P04-51100P04-51500P04-53500P04-54010PP04-54050PP04-54500P04-60100P04-60125

9595959595959494949494949494949494474747474747474747939394969696969595959595959595969694969696

105106

P04-60225P04-60500P04-63100P04-63225P04-63500P04-64100P04-64500P04-65100P04-65500P04-67100P04-67500P04-68100P04-68500P04-70100P04-70101P04-70200P04-70201P04-710100P04-710110P04-710200P04-710210P04-710401P04-71040MP04-710411P04-710411MP04-710412 P04-710412MP04-710413P04-710416P04-710608P04-710608MP04-710609 P04-710609MP04-710613 P04-710613MP04-710701 P04-710701MP04-710801P04-710PX10P04-710PX40P04-714000 P04-714000MP04-71411SP04-716000 P04-716000MP04-718000 P04-718000MP04-740500P04-7411S0

106105105106105105105105105105105105105

767677772828333321212222232323232727272728282929293031242422262625257422

P04-7411S1P04-77010KP04-7701KP04-77020KP04-7702KP04-77110KP04-7711KP04-77120KP04-7712KP04-77130KP04-7713KP04-77140KP04-7714KP04-77210KP04-77250KP04-77310KP04-77350KP04-77410KP04-77450KP04-7751P04-77510KP04-77620KP04-7762KP04-777500P04-77750KP04-80025PP04-80050P04-80053P04-80054P04-80057P04-80100P04-80100PP04-80500P04-80500PP04-81500P04-82010PP04-82050P04-82100P04-82500P04-83500P04-84500P04-84600P04-850100P04-850500P04-85600P04-90065SP04-90500P04-91500P04-92500

228181828284848585868687878383888889898181828290909797585858979769974997979749694141323254

107676768


Index


Index


Product No. Page Product No. Page Product No. Page Product No. PageP04-93500P04-951SA2P04-95700P04-95750P04-95800P04-95850P04-96500P04-97500P04-98500P04-991000P04-991500P04-992000P05-01100P05-01100PP05-01500P05-01500PP05-39500P06-01001P06-01005P06-01050P06-01050PP06-01100P06-01100PP06-01225PP06-01250PP06-02010PP06-02025PP06-02100P06-03001P06-03001PP06-03004P06-03005P06-03010PP06-03025PP06-03050P06-03100P06-04001P06-04005P06-04010PP06-04050P06-04050PP06-04100P06-05050P06-05100P06-06010PP06-06025PP06-06050P06-06100P06-06100P

68201818191948484893939393939393939898989898989898989898989898989898989898989898989898989898989898

P06-07001P06-07005P06-07050P06-07100P06-07300P06-07301P06-07305P06-07350P06-07800P06-080050PP06-080100PP06-08020P06-08025PP06-08100P06-08100PP06-0849010P06-0849050P06-09050P06-11025PP06-11050PP06-11100PP06-12001P06-12005P06-12050P06-12100P06-13001P06-13005P06-13050P06-13100P06-138110P06-138150P06-138210P06-138250P06-138310P06-138350P06-138410P06-138450P06-1391050P06-1391100P06-1391500P06-139210P06-139250P06-139310P06-139350P06-139410P06-139450P06-14001P06-14005P06-14050

999999999999999998989898999899

104104

999999999999999998989898

104104104104104104104104104104104104104104104104104

989898

P06-14100P06-15001P06-15005P06-15050P06-15100P06-16001PP06-16005PP06-16010PP06-16020P06-16100P06-16220P06-20020P06-20030P06-20100P06-20166P06-20410P06-20501P06-20650P06-21000P06-21100P06-21500P06-25200P06-26025P06-26050P06-28010P07-01100P07-02100P07-03100P07-03110P07-03200P07-03210P07-03300P07-03310P07-03400P07-03410P07-04100P07-04200P07-04300P07-05020P07-05100P07-07010P07-07100P07-07200P07-07210P07-10010P07-10100P07-11010P07-11100P07-20001

9898989898999999999999

102102102102102103107

939393

102104104

99939393939393939393939393939393757575757575757575

P07-20021P07-90050P07-91010P07-92010P07-92050P07-94050P08-02100P08-2000P08-30100P08-31100P08-32100P08-40100P08-41100P08-50500P08-50600P10-019100P10-019500P10-020100P10-020500P10-021100P10-021500P10-022100P10-022500P10-023100P10-0231SFP10-0231SPP10-023500P10-0235SPP10-024100P10-024500P10-025025PP10-025100P10-025100PP10-025500P10-025500PP10-026100P10-026500P10-027100P10-027500P10-028100P10-029100P10-029500P10-031100P10-032100P10-033100P10-0331SFP10-038100P10-038500P10-040100

75108

93108108107

939797979797977575

101101101101101101101101101107101101101101101101101101101101101101101101101101101

93101101107101101101


Product No. Page Product No. Page Product No. Page Product No. PageP10-040500P10-100100P10-21100P-2060020P-2060100P-2061000P-2360011P-2360012P-2360013P-2701001P-2701002P30-0101P30-0102P30-01901P30-01901EP30-0200P30-0201P30-0210P30-0301P30-0302P30-0401P30-0402P30-0601P30-0602P30-0701P30-0702P30-0801P30-0802P30-0901P30-0902P30-1001P30-1002P30-1101P30-1102P30-1301P30-1302P30-1401P30-1402P30-1501P30-1502P30-1505P30-1506P30-1701P30-1702P30-1905P30-1906P30-1908P30-1909P30-1981

101101100117117117117117117116116

111111111111111111

5555

11111111111111111111

5555555555

1010101010

P30-1985P30-2008P30-2009P30-2081P30-2085P30-2101P30-2102P30-2301P30-2302P30-2401P30-2402P30-2501P30-2502P30-2601P30-2602P30-2605P30-2606P30-2608P30-2609P30-2701P30-2702P30-2801P30-2802P30-2901P30-2902P30-3301P30-3302P30-3305P30-3306P30-3401P30-3402P30-3601P30-3602P30-4101P30-4102P30-5100P30-5500P30-8100P30-8500P-3340002P-3610002P-3860001P-3860002P40-1301P40-1302P40-1401P40-1402P40-37100P40-37500

10101010101010101012121212

777777

121210101212

5555

101010101111

5555

120116119119

777766

P40-38100P40-38500P40-39100P40-39500P40-58100P40-58500P40-59100P40-59500P-4390002P-4390010P60-15840100P60-36720100PAN721064PAN721065PAN733025PAN733026PAN737042PAN737046PAN739025PAN739026PAN739028PAN739043PAN741025PANsys3000

66666666

119119108108127127129129130130132132133133131135

PAN-Biotech GmbHAm Gewerbepark 13D-94501 Aidenbach

GERMANY

Phone: +49 (0)8543/6016-30Fax: +49 (0)8543/6016-49

www.pan-biotech.dewww.pan-biotech.com

INTERNATIONAL MEDICAL PRODUCTSWaversesteenweg 1110, box 7

1160 Brussels - BELGIUM

T: +32 (0)2 660 50 75 - F: +32 (0)2 660 20 [email protected] - www.intermed.be

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