© 2013 pearson education, inc. chapter 6: microbial growth $100 $200 $300 $400 $500 $100$100$100...
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© 2013 Pearson Education, Inc.
Chapter 6: Microbial Growth
$100
$200
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$500
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Physical Requirements
ChemicalRequirements
Growth of Bacterial Cultures
Culture MediaMeasurement
of Growth
FINAL ROUND
© 2013 Pearson Education, Inc.
Topic 1: Physical Requirements
$100 Question
The optimum pH for most bacteria is
a. 1.
b. 5.
c. 7.
d. 9.
ANSWER
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 1: Physical Requirements
$100 Answer
The optimum pH for most bacteria is
a. 1.
b. 5.
c. 7.
d. 9.
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 1: Physical Requirements
$200 Question
A psychrophile grows best at
a. 37°C.
b. 10°C.
c. 25°C.
d. 15°C.
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ANSWER
© 2013 Pearson Education, Inc.
Topic 1: Physical Requirements
$200 Answer
A psychrophile grows best at
a. 37°C.
b. 10°C.
c. 25°C.
d. 15°C.
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 1: Physical Requirements
$300 Question
Organisms that require high salt concentrations for growth are called
a. halophiles.
b. extreme halophiles.
c. facultative halophiles.
d. microhalophiles.
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ANSWER
© 2013 Pearson Education, Inc.
Topic 1: Physical Requirements
$300 Answer
Organisms that require high salt concentrations for growth are called
a. halophiles.
b. extreme halophiles.
c. facultative halophiles.
d. microhalophiles.
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Topic 1: Physical Requirements
$400 Question
What is the energy source utilized by producers in the hydrothermal vents on theocean floor?
a. light
b. hydrogen sulfide
c. organic molecules
d. carbon dioxide
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ANSWER
© 2013 Pearson Education, Inc.
Topic 1: Physical Requirements
$400 Answer
What is the energy source utilized byproducers in the hydrothermal vents on theocean floor?
a. light
b. hydrogen sulfide
c. organic molecules
d. carbon dioxide
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 1: Physical Requirements
$500 Question
In food preservation, salts and sugars areused because they create a
a. lower pH.
b. hypertonic environment.
c. hypotonic environment.
d. lower osmotic pressure.
BACK TO GAME
ANSWER
© 2013 Pearson Education, Inc.
Topic 1: Physical Requirements
$500 Answer
In food preservation, salts and sugars areused because they create a
a. lower pH.
b. hypertonic environment.
c. hypotonic environment.
d. lower osmotic pressure.
BACK TO GAME
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Chemoautotrophs and photoautotrophs derive their carbon from
a. carbon dioxide.
b. proteins.
c. lipids.
d. carbohydrates.
Topic 2: Chemical Requirements
$100 Question
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ANSWER
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Topic 2: Chemical Requirements
$100 Answer
Chemoautotrophs and photoautotrophs derive their carbon from
a. carbon dioxide.
b. proteins.
c. lipids.
d. carbohydrates.
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Some microorganisms use gaseous nitrogen directly from the atmosphere in a process called
a. chemosynthesis.
b. photosynthesis.
c. nitrogen fixation.
d. nitrogen synthesis.
Topic 2: Chemical Requirements
$200 Question
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ANSWER
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Some microorganisms use gaseous nitrogen directly from the atmosphere in a process called
a. chemosynthesis.
b. photosynthesis.
c. nitrogen fixation.
d. nitrogen synthesis.
Topic 2: Chemical Requirements
$200 Answer
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An organism that uses oxygen but can grow without oxygen is called a(n)
a. obligate aerobe.
b. facultative aerobe.
c. aerobe.
d. aerotolerant anaerobe.
Topic 2: Chemical Requirements
$300 Question
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ANSWER
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An organism that uses oxygen but can grow without oxygen is called a(n)
a. obligate aerobe.
b. facultative aerobe.
c. aerobe.
d. aerotolerant anaerobe.
Topic 2: Chemical Requirements
$300 Answer
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Topic 2: Chemical Requirements
$400 Question
BACK TO GAME
ANSWER
Which organism is killed by atmospheric oxygen?
a. aerobe
b. obligate aerobe
c. obligate anaerobe
d. aerotolerant anaerobe
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Topic 2: Chemical Requirements
$400 Answer
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Which organism is killed by atmosphericoxygen?
a. aerobe
b. obligate aerobe
c. obligate anaerobe
d. aerotolerant anaerobe
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Topic 2: Chemical Requirements
$500 Question
Biofilms reside in a matrix made up primarily of
a. lipids.
b. hydrogel.
c. proteins.
d. polysaccharides.
BACK TO GAME
ANSWER
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Topic 2: Chemical Requirements
$500 Answer
Biofilms reside in a matrix made up primarily of
a. lipids.
b. hydrogel.
c. proteins.
d. polysaccharides.
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Topic 3: Growth of Bacterial Cultures
$100 Question
Generation time can best be defined as the
a. length of time it takes for lag phase.
b. duration of log phase.
c. length of time it takes for a cell to divide.
d. minimum rate of doubling.
BACK TO GAME
ANSWER
© 2013 Pearson Education, Inc.
Topic 3: Growth of Bacterial Cultures
$100 Answer
Generation time can best be defined as the
a. length of time it takes for lag phase.
b. duration of log phase.
c. length of time it takes for a cell to divide.
d. minimum rate of doubling.
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© 2013 Pearson Education, Inc.
Topic 3: Growth of Bacterial Cultures
$200 Question
How many cells would be present after fourgenerations if we started with three cells?
a. 12
b. 24
c. 48
d. 96
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ANSWER
© 2013 Pearson Education, Inc.
Topic 3: Growth of Bacterial Cultures
$200 Answer
How many cells would be present after fourgenerations if we started with three cells?
a. 12
b. 24
c. 48
d. 96
BACK TO GAME
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In which growth phase does the number of cells change very little because cells do NOT immediately reproduce in a new medium?
a. stationary phase
b. log phase
c. death phase
d. lag phase
Topic 3: Growth of Bacterial Cultures
$300 Question
BACK TO GAME
ANSWER
© 2013 Pearson Education, Inc.
Topic 3: Growth of Bacterial Cultures
$300 Answer
In which growth phase does the number of cells change very little because cells do NOT immediately reproduce in a new medium?
a. stationary phase
b. log phase
c. death phase
d. lag phase
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 3: Growth of Bacterial Cultures
$400 Question
Which of the following is NOT a possible reason why exponential growth stops?
a. accumulation of waste products
b. exhaustion of nutrients
c. harmful changes in pH
d. All of these are possible reasons.
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ANSWER
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Topic 3: Growth of Bacterial Cultures
$400 Answer
BACK TO GAME
Which of the following is NOT a possible reason why exponential growth stops?
a. accumulation of waste products
b. exhaustion of nutrients
c. harmful changes in pH
d. All of these are possible reasons.
© 2013 Pearson Education, Inc.
Topic 3: Growth of Bacterial Cultures
$500 Question
In the stationary phase,
a. the number of microbial deaths exceeds the number of new cells formed.
b. the number of microbial deaths is equal to the number of new cells formed.
c. new cells are no longer formed because of adverse environmental conditions.
d. cellular reproduction is most active.
BACK TO GAME
ANSWER
© 2013 Pearson Education, Inc.
Topic 3: Growth of Bacterial Cultures
$500 Answer
In the stationary phase,
a. the number of microbial deaths exceeds the number of new cells formed.
b. the number of microbial deaths is equal to the number of new cells formed.
c. new cells are no longer formed because of adverse environmental conditions.
d. cellular reproduction is most active.
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 4: Culture Media
$100 Question
Which type of medium suppresses the growth of unwanted bacteria and distinguishes colonies of the desired organisms from other organisms?
a. selective media
b. complex media
c. selective and differential media
d. enrichment mediaBACK TO GAME
ANSWER
© 2013 Pearson Education, Inc.
Topic 4: Culture Media
$100 Answer
Which type of medium suppresses the growth of unwanted bacteria and distinguishes colonies of the desired organisms from other organisms?
a. selective media
b. complex media
c. selective and differential media
d. enrichment mediaBACK TO GAME
© 2013 Pearson Education, Inc.
Topic 4: Culture Media
$200 Question
The microbes that grow and multiply in or on a culture medium are referred to as a(n)
a. culture.
b. inoculum.
c. specimen.
d. medium.
ANSWER
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© 2013 Pearson Education, Inc.
Topic 4: Culture Media
$200 Answer
The microbes that grow and multiply in or on a culture medium are referred to as a(n)
a. culture.
b. inoculum.
c. specimen.
d. medium.
BACK TO GAME
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Topic 4: Culture Media
$300 Question
What can be added to a medium when it is desirable to grow bacteria on a solid medium?
a. glucose
b. magnesium sulfate
c. potassium phosphate
d. agar
BACK TO GAME
ANSWER
© 2013 Pearson Education, Inc.
Topic 4: Culture Media
$300 Answer
What can be added to a medium when it is desirable to grow bacteria on a solid medium?
a. glucose
b. magnesium sulfate
c. potassium phosphate
d. agar
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 4: Culture Media
$400 Question
A culture medium consisting of agar, human blood, and beef heart is a _____ medium.
a. complex
b. selective
c. differential
d. chemically defined
BACK TO GAME
ANSWER
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Topic 4: Culture Media
$400 Answer
BACK TO GAME
A culture medium consisting of agar, human blood, and beef heart is a _____ medium.
a. complex
b. selective
c. differential
d. chemically defined
© 2013 Pearson Education, Inc.
Topic 4: Culture Media
$500 Question
The source of nutrients in nutrient agar is
a. agar.
b. peptone and sodium chloride.
c. peptone and beef extract.
d. nutrient.
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ANSWER
© 2013 Pearson Education, Inc.
Topic 4: Culture Media
$500 Answer
The source of nutrients in nutrient agar is
a. agar.
b. peptone and sodium chloride.
c. peptone and beef extract.
d. nutrient.
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 5: Measurement of Growth
$100 Question
A spectrophotometer can be used to measure
a. metabolic activity.
b. turbidity.
c. dry weight.
d. pathogenicity.
BACK TO GAME
ANSWER
© 2013 Pearson Education, Inc.
Topic 5: Measurement of Growth
$100 Answer
A spectrophotometer can be used to measure
a. metabolic activity.
b. turbidity.
c. dry weight.
d. pathogenicity.
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 5: Measurement of Growth
$200 Question
Because of time considerations, which method is usually used to count the number of bacteria in milk?
a. the direct microscope count method
b. the pour plate method
c. the spread plate method
d. the most probable number method
BACK TO GAME
ANSWER
© 2013 Pearson Education, Inc.
Topic 5: Measurement of Growth
$200 Answer
Because of time considerations, which method is usually used to count the number of bacteria in milk?
a. the direct microscope count method
b. the pour plate method
c. the spread plate method
d. the most probable number method
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 5: Measurement of Growth
$300 Question
Which method is utilized to ensure that plates containing 25 to 250 colonies are counted?
a. the most probable number method
b. serial dilution
c. filtration
d. spread plate method
BACK TO GAME
ANSWER
© 2013 Pearson Education, Inc.
Topic 5: Measurement of Growth
$300 Answer
BACK TO GAME
Which method is utilized to ensure that plates containing 25 to 250 colonies are counted?
a. the most probable number method
b. serial dilution
c. filtration
d. spread plate method
© 2013 Pearson Education, Inc.
Topic 5: Measurement of Growth
$400 Question
Which is the best technique to use to measure the growth of filamentous organisms?
a. most probable number method
b. direct microscopic count
c. turbidity
d. dry weight
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ANSWER
© 2013 Pearson Education, Inc.
Topic 5: Measurement of Growth
$400 Answer
Which is the best technique to use to measure the growth of filamentous organisms?
a. most probable number method
b. direct microscopic count
c. turbidity
d. dry weight
BACK TO GAME
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Topic 5: Measurement of Growth
$500 Question
Which technique is frequently used to detect and enumerate coliform bacteria?
a. turbidity
b. spread plate method
c. filtration
d. pour plate method
BACK TO GAME
ANSWER
© 2013 Pearson Education, Inc.
Topic 5: Measurement of Growth
$500 Answer
Which technique is frequently used to detect and enumerate coliform bacteria?
a. turbidity
b. spread plate method
c. filtration
d. pour plate method
BACK TO GAME
© 2013 Pearson Education, Inc.
FINAL ROUND Question
An experiment began with 5 cells and ended with 160 cells. How many generations did the cells go through?
a. 32
b. 6
c. 4
d. 5
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ANSWER