© 2013 pearson education, inc. chapter 6: microbial growth $100 $200 $300 $400 $500 $100$100$100...

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2013 Pearson Education, Inc. Chapter 6: Microbial Growth $10 0 $20 0 $30 0 $40 0 $50 0 $10 0 $ 100 $10 0 $100 $20 0 $20 0 $20 0 $200 $30 0 $30 0 $30 0 $300 $40 0 $40 0 $40 0 $400 $50 0 $50 0 $50 0 $500 Physical Requirements Chemical Requirements Growth of Bacterial Cultures Culture Media Measurement of Growth FINAL ROUND

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© 2013 Pearson Education, Inc.

Chapter 6: Microbial Growth

$100

$200

$300

$400

$500

$100 $100$100 $100

$200 $200 $200 $200

$300 $300 $300 $300

$400 $400 $400 $400

$500 $500 $500 $500

Physical Requirements

ChemicalRequirements

Growth of Bacterial Cultures

Culture MediaMeasurement

of Growth

FINAL ROUND

© 2013 Pearson Education, Inc.

Topic 1: Physical Requirements

$100 Question

The optimum pH for most bacteria is

a. 1.

b. 5.

c. 7.

d. 9.

ANSWER

BACK TO GAME

© 2013 Pearson Education, Inc.

Topic 1: Physical Requirements

$100 Answer

The optimum pH for most bacteria is

a. 1.

b. 5.

c. 7.

d. 9.

BACK TO GAME

© 2013 Pearson Education, Inc.

Topic 1: Physical Requirements

$200 Question

A psychrophile grows best at

a. 37°C.

b. 10°C.

c. 25°C.

d. 15°C.

BACK TO GAME

ANSWER

© 2013 Pearson Education, Inc.

Topic 1: Physical Requirements

$200 Answer

A psychrophile grows best at

a. 37°C.

b. 10°C.

c. 25°C.

d. 15°C.

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© 2013 Pearson Education, Inc.

Topic 1: Physical Requirements

$300 Question

Organisms that require high salt concentrations for growth are called

a. halophiles.

b. extreme halophiles.

c. facultative halophiles.

d. microhalophiles.

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ANSWER

© 2013 Pearson Education, Inc.

Topic 1: Physical Requirements

$300 Answer

Organisms that require high salt concentrations for growth are called

a. halophiles.

b. extreme halophiles.

c. facultative halophiles.

d. microhalophiles.

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© 2013 Pearson Education, Inc.

Topic 1: Physical Requirements

$400 Question

What is the energy source utilized by producers in the hydrothermal vents on theocean floor?

a. light

b. hydrogen sulfide

c. organic molecules

d. carbon dioxide

BACK TO GAME

ANSWER

© 2013 Pearson Education, Inc.

Topic 1: Physical Requirements

$400 Answer

What is the energy source utilized byproducers in the hydrothermal vents on theocean floor?

a. light

b. hydrogen sulfide

c. organic molecules

d. carbon dioxide

BACK TO GAME

© 2013 Pearson Education, Inc.

Topic 1: Physical Requirements

$500 Question

In food preservation, salts and sugars areused because they create a

a. lower pH.

b. hypertonic environment.

c. hypotonic environment.

d. lower osmotic pressure.

BACK TO GAME

ANSWER

© 2013 Pearson Education, Inc.

Topic 1: Physical Requirements

$500 Answer

In food preservation, salts and sugars areused because they create a

a. lower pH.

b. hypertonic environment.

c. hypotonic environment.

d. lower osmotic pressure.

BACK TO GAME

© 2013 Pearson Education, Inc.

Chemoautotrophs and photoautotrophs derive their carbon from

a. carbon dioxide.

b. proteins.

c. lipids.

d. carbohydrates.

Topic 2: Chemical Requirements

$100 Question

BACK TO GAME

ANSWER

© 2013 Pearson Education, Inc.

Topic 2: Chemical Requirements

$100 Answer

Chemoautotrophs and photoautotrophs derive their carbon from

a. carbon dioxide.

b. proteins.

c. lipids.

d. carbohydrates.

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© 2013 Pearson Education, Inc.

Some microorganisms use gaseous nitrogen directly from the atmosphere in a process called

a. chemosynthesis.

b. photosynthesis.

c. nitrogen fixation.

d. nitrogen synthesis.

Topic 2: Chemical Requirements

$200 Question

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ANSWER

© 2013 Pearson Education, Inc.

Some microorganisms use gaseous nitrogen directly from the atmosphere in a process called

a. chemosynthesis.

b. photosynthesis.

c. nitrogen fixation.

d. nitrogen synthesis.

Topic 2: Chemical Requirements

$200 Answer

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© 2013 Pearson Education, Inc.

An organism that uses oxygen but can grow without oxygen is called a(n)

a. obligate aerobe.

b. facultative aerobe.

c. aerobe.

d. aerotolerant anaerobe.

Topic 2: Chemical Requirements

$300 Question

BACK TO GAME

ANSWER

© 2013 Pearson Education, Inc.

An organism that uses oxygen but can grow without oxygen is called a(n)

a. obligate aerobe.

b. facultative aerobe.

c. aerobe.

d. aerotolerant anaerobe.

Topic 2: Chemical Requirements

$300 Answer

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© 2013 Pearson Education, Inc.

Topic 2: Chemical Requirements

$400 Question

BACK TO GAME

ANSWER

Which organism is killed by atmospheric oxygen?

a. aerobe

b. obligate aerobe

c. obligate anaerobe

d. aerotolerant anaerobe

© 2013 Pearson Education, Inc.

Topic 2: Chemical Requirements

$400 Answer

BACK TO GAME

Which organism is killed by atmosphericoxygen?

a. aerobe

b. obligate aerobe

c. obligate anaerobe

d. aerotolerant anaerobe

© 2013 Pearson Education, Inc.

Topic 2: Chemical Requirements

$500 Question

Biofilms reside in a matrix made up primarily of

a. lipids.

b. hydrogel.

c. proteins.

d. polysaccharides.

BACK TO GAME

ANSWER

© 2013 Pearson Education, Inc.

Topic 2: Chemical Requirements

$500 Answer

Biofilms reside in a matrix made up primarily of

a. lipids.

b. hydrogel.

c. proteins.

d. polysaccharides.

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© 2013 Pearson Education, Inc.

Topic 3: Growth of Bacterial Cultures

$100 Question

Generation time can best be defined as the

a. length of time it takes for lag phase.

b. duration of log phase.

c. length of time it takes for a cell to divide.

d. minimum rate of doubling.

BACK TO GAME

ANSWER

© 2013 Pearson Education, Inc.

Topic 3: Growth of Bacterial Cultures

$100 Answer

Generation time can best be defined as the

a. length of time it takes for lag phase.

b. duration of log phase.

c. length of time it takes for a cell to divide.

d. minimum rate of doubling.

BACK TO GAME

© 2013 Pearson Education, Inc.

Topic 3: Growth of Bacterial Cultures

$200 Question

How many cells would be present after fourgenerations if we started with three cells?

a. 12

b. 24

c. 48

d. 96

BACK TO GAME

ANSWER

© 2013 Pearson Education, Inc.

Topic 3: Growth of Bacterial Cultures

$200 Answer

How many cells would be present after fourgenerations if we started with three cells?

a. 12

b. 24

c. 48

d. 96

BACK TO GAME

© 2013 Pearson Education, Inc.

In which growth phase does the number of cells change very little because cells do NOT immediately reproduce in a new medium?

a. stationary phase

b. log phase

c. death phase

d. lag phase

Topic 3: Growth of Bacterial Cultures

$300 Question

BACK TO GAME

ANSWER

© 2013 Pearson Education, Inc.

Topic 3: Growth of Bacterial Cultures

$300 Answer

In which growth phase does the number of cells change very little because cells do NOT immediately reproduce in a new medium?

a. stationary phase

b. log phase

c. death phase

d. lag phase

BACK TO GAME

© 2013 Pearson Education, Inc.

Topic 3: Growth of Bacterial Cultures

$400 Question

Which of the following is NOT a possible reason why exponential growth stops?

a. accumulation of waste products

b. exhaustion of nutrients

c. harmful changes in pH

d. All of these are possible reasons.

BACK TO GAME

ANSWER

© 2013 Pearson Education, Inc.

Topic 3: Growth of Bacterial Cultures

$400 Answer

BACK TO GAME

Which of the following is NOT a possible reason why exponential growth stops?

a. accumulation of waste products

b. exhaustion of nutrients

c. harmful changes in pH

d. All of these are possible reasons.

© 2013 Pearson Education, Inc.

Topic 3: Growth of Bacterial Cultures

$500 Question

In the stationary phase,

a. the number of microbial deaths exceeds the number of new cells formed.

b. the number of microbial deaths is equal to the number of new cells formed.

c. new cells are no longer formed because of adverse environmental conditions.

d. cellular reproduction is most active.

BACK TO GAME

ANSWER

© 2013 Pearson Education, Inc.

Topic 3: Growth of Bacterial Cultures

$500 Answer

In the stationary phase,

a. the number of microbial deaths exceeds the number of new cells formed.

b. the number of microbial deaths is equal to the number of new cells formed.

c. new cells are no longer formed because of adverse environmental conditions.

d. cellular reproduction is most active.

BACK TO GAME

© 2013 Pearson Education, Inc.

Topic 4: Culture Media

$100 Question

Which type of medium suppresses the growth of unwanted bacteria and distinguishes colonies of the desired organisms from other organisms?

a. selective media

b. complex media

c. selective and differential media

d. enrichment mediaBACK TO GAME

ANSWER

© 2013 Pearson Education, Inc.

Topic 4: Culture Media

$100 Answer

Which type of medium suppresses the growth of unwanted bacteria and distinguishes colonies of the desired organisms from other organisms?

a. selective media

b. complex media

c. selective and differential media

d. enrichment mediaBACK TO GAME

© 2013 Pearson Education, Inc.

Topic 4: Culture Media

$200 Question

The microbes that grow and multiply in or on a culture medium are referred to as a(n)

a. culture.

b. inoculum.

c. specimen.

d. medium.

ANSWER

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© 2013 Pearson Education, Inc.

Topic 4: Culture Media

$200 Answer

The microbes that grow and multiply in or on a culture medium are referred to as a(n)

a. culture.

b. inoculum.

c. specimen.

d. medium.

BACK TO GAME

© 2013 Pearson Education, Inc.

Topic 4: Culture Media

$300 Question

What can be added to a medium when it is desirable to grow bacteria on a solid medium?

a. glucose

b. magnesium sulfate

c. potassium phosphate

d. agar

BACK TO GAME

ANSWER

© 2013 Pearson Education, Inc.

Topic 4: Culture Media

$300 Answer

What can be added to a medium when it is desirable to grow bacteria on a solid medium?

a. glucose

b. magnesium sulfate

c. potassium phosphate

d. agar

BACK TO GAME

© 2013 Pearson Education, Inc.

Topic 4: Culture Media

$400 Question

A culture medium consisting of agar, human blood, and beef heart is a _____ medium.

a. complex

b. selective

c. differential

d. chemically defined

BACK TO GAME

ANSWER

© 2013 Pearson Education, Inc.

Topic 4: Culture Media

$400 Answer

BACK TO GAME

A culture medium consisting of agar, human blood, and beef heart is a _____ medium.

a. complex

b. selective

c. differential

d. chemically defined

© 2013 Pearson Education, Inc.

Topic 4: Culture Media

$500 Question

The source of nutrients in nutrient agar is

a. agar.

b. peptone and sodium chloride.

c. peptone and beef extract.

d. nutrient.

BACK TO GAME

ANSWER

© 2013 Pearson Education, Inc.

Topic 4: Culture Media

$500 Answer

The source of nutrients in nutrient agar is

a. agar.

b. peptone and sodium chloride.

c. peptone and beef extract.

d. nutrient.

BACK TO GAME

© 2013 Pearson Education, Inc.

Topic 5: Measurement of Growth

$100 Question

A spectrophotometer can be used to measure

a. metabolic activity.

b. turbidity.

c. dry weight.

d. pathogenicity.

BACK TO GAME

ANSWER

© 2013 Pearson Education, Inc.

Topic 5: Measurement of Growth

$100 Answer

A spectrophotometer can be used to measure

a. metabolic activity.

b. turbidity.

c. dry weight.

d. pathogenicity.

BACK TO GAME

© 2013 Pearson Education, Inc.

Topic 5: Measurement of Growth

$200 Question

Because of time considerations, which method is usually used to count the number of bacteria in milk?

a. the direct microscope count method

b. the pour plate method

c. the spread plate method

d. the most probable number method

BACK TO GAME

ANSWER

© 2013 Pearson Education, Inc.

Topic 5: Measurement of Growth

$200 Answer

Because of time considerations, which method is usually used to count the number of bacteria in milk?

a. the direct microscope count method

b. the pour plate method

c. the spread plate method

d. the most probable number method

BACK TO GAME

© 2013 Pearson Education, Inc.

Topic 5: Measurement of Growth

$300 Question

Which method is utilized to ensure that plates containing 25 to 250 colonies are counted?

a. the most probable number method

b. serial dilution

c. filtration

d. spread plate method

BACK TO GAME

ANSWER

© 2013 Pearson Education, Inc.

Topic 5: Measurement of Growth

$300 Answer

BACK TO GAME

Which method is utilized to ensure that plates containing 25 to 250 colonies are counted?

a. the most probable number method

b. serial dilution

c. filtration

d. spread plate method

© 2013 Pearson Education, Inc.

Topic 5: Measurement of Growth

$400 Question

Which is the best technique to use to measure the growth of filamentous organisms?

a. most probable number method

b. direct microscopic count

c. turbidity

d. dry weight

BACK TO GAME

ANSWER

© 2013 Pearson Education, Inc.

Topic 5: Measurement of Growth

$400 Answer

Which is the best technique to use to measure the growth of filamentous organisms?

a. most probable number method

b. direct microscopic count

c. turbidity

d. dry weight

BACK TO GAME

© 2013 Pearson Education, Inc.

Topic 5: Measurement of Growth

$500 Question

Which technique is frequently used to detect and enumerate coliform bacteria?

a. turbidity

b. spread plate method

c. filtration

d. pour plate method

BACK TO GAME

ANSWER

© 2013 Pearson Education, Inc.

Topic 5: Measurement of Growth

$500 Answer

Which technique is frequently used to detect and enumerate coliform bacteria?

a. turbidity

b. spread plate method

c. filtration

d. pour plate method

BACK TO GAME

© 2013 Pearson Education, Inc.

FINAL ROUND Question

An experiment began with 5 cells and ended with 160 cells. How many generations did the cells go through?

a. 32

b. 6

c. 4

d. 5

BACK TO GAME

ANSWER

© 2013 Pearson Education, Inc.

FINAL ROUND Answer

An experiment began with 5 cells and ended with 160 cells. How many generations did the cells go through?

a. 32

b. 6

c. 4

d. 5

BACK TO GAME