FREQUENTLY ASKED QUESTIONS

ZEIGLER COMPLETE HATCHERY LINE

What percent of natural Artemia will it replace?In shrimp production trials, EZ Artemia demonstrated 100% replacement of Artemia and gave better survivals, larger PLs and faster growth. EZ Artemia consistently provides survivals of greater than 70%.

How do you feed it?For best results, feed the recommended amount of EZ Artemia every two hours. 1) Shake the jug well, 2) Measure into a beaker, 3) Dilute 1 part EZ Artemia with 10 parts water, 4) Swirl to mix well, and 5) Distribute evenly throughout the tank. Nothing could be EZer!

How do feeding rates of EZ Artemia and Artemia compare?1 kg of EZ Artemia replaces 1 kg of Artemia cysts (80% hatch-out) or the resulting 2-3 kg of wet weight Artemia derived from 1 kg of cysts. EZ Artemia is designed to replicate enriched Artemia and therefore has a superior nutrient profile and fatty acid (HUFA) composition than regular Artemia.

How does this product save the hatchery money?EZ Artemia does not require the extra personnel, tanks, pumps, blowers and freezer space associated with Artemia which can add up to $15/kg in additional costs. It also can cut up to 3 days off of the culture cycle, saving several hundred dollars/million PLs produced in cost savings. Finally, unlike Artemia, it is not subject to supply limitations and the associated price instabilities.

How can EZ Artemia help with biosecurity in a hatchery?Hatched Artemia is a known vector of many very pathogenic bacteria. Using EZ Artemia reduces the introduction of pathogens into your production tank because it is free of all known shrimp pathogens. VpakTM (Vitality Pak) is included in the formulation to help boost immune response and resist disease. Anti-microbial probiotics are included to inhibit pathogens.

Does this product need any special storage such as with Artemia?EZ Artemia has a shelf life of up to 2 years if kept in a cool location (22° C/77° F). It can be refrigerated, but not frozen. Open containers can be used up to 6 weeks.

Larval Esencial

Larval Z-Plus

Larval AP-100

Artemia Flakes

Shrimp Broodstock

Shrimp Maturation

EZ Black

Shrimp Starter

EZ MateMaturation & Broodstock

EZ LarvaLarval Feeds

PL Raceway PlusPost-Larval Feeds

VpakTM

BiopakTM

EZ BioHealth Enhancement

Pre-ground, Artemia flake to enhance monitoring of feeding and animal activity through coloration of the digestive tract

Cost-effective post-larval feed utilizing highly-digestible marine ingredients

Complete, premium diet to enhance growth and produce stronger PLs for acclimation into ponds

Well-proven larval feed formulated to support good health and survivals in fish and fresh water shrimp larvae

Cost-effective dry larval feed based utilizing marine ingredients and providing enhanced attractability

Premium dry larval feed utilizing high levels of HUFAs and cholesterol to promote molting and growth

Highly attractable flakes formulated with brine shrimp; Available in black and red colors providing enhanced pigmentation

Highly palatable diet formulated to reduce lag time associated ovarian maturation

Complete pelleted diet supporting transition from growout to Broodstock. Contains high levels of marine proteins & oils to reduce time to maturation

Pond probiotic or feed supplement designed to reduce organic sludge and improve water quality in ponds

Partial replacement of fresh feeds; contains high levels of HUFAs and astaxanthan. Proven to increase spawning frequency, as well as quantity and quality of nauplii

Probiotic designed to reduce vibrio and other pathogens in hatchery rearing tanks

Feed supplement to increase survivals during stressful conditions in ponds

Premium, micro-capsulated, liquid larval feed designed to improve water quality, shorten the culture cycle, and produce stronger PLs

Zeigler Bros., Inc. P.O. Box 95

Gardners, PA 17324 USA717-677-6181 phonewww.zeiglerfeed.com

Zeigler Aquaculture © 2012

A Proven Alternative to Natural Artemia

Micro-capsulated Liquid Larval DietEZZeigler Tradition

Setting aside their orchestra careers touring as The Scotch Highlanders, two brothers, Ty and LeRoy Zeigler, moved their young families to rural Gettysburg, Pennsylvania, where they had just purchased a farm and a water-powered grist feed mill. The year was 1935.

The first feeds were manufactured under the Conewago brand, named after the stream which powered their newly purchased mill. Their strong orientation to value, integrity and customer service was soon rewarded by the trust and business of many local farmers.

In 1967, the leadership passed to LeRoy’s son, Dr. Tom Zeigler, who transitioned the company from a manufacturer of farm feeds to an internationally recognized producer of specialized animal feeds. Beginning in the 1980s, the company began licensing nutritional and manufacturing technologies globally under its Zeigler brand.

The Zeigler family tradition continues today, more than 75 years later, under the leadership of Tom’s sons, Tim and Matt Zeigler. With a renewed spirit toward technology and innovation, the brothers continue to uphold the company traditions of value and integrity, while striving to serve the changing needs of loyal customers.

Circa 1950’s.

Worldwide demand for Artemia cysts continues to rise to levels which could exceed global supply (see below). In past years, good harvests and adequate inventories have helped Artemia suppliers meet market demands. Today, as global demand increases, the combination of depleted inventories and unpredictable harvests could result in supply shortfalls much sooner than anticipated.

It is important that the industry understands Artemia’s limitations and continues to focus its efforts to reduce the risks and costs of excessive reliance on this natural resource. Developing practical and cost-effective alternatives to Artemia will clearly help aquaculture become a more sustainable means to feed our planet.

Zeigler Bros., Inc. is a pioneer and leader in the development and formulation of specialized feeds for the global aquaculture industry. Our commitment is to the worldwide growth of aquaculture through continued researchand development. No matter the species or production system, we are experienced and equipped to providethe solutions you need to improve the health and productivity of your aquaculture operation.

EZ ARTEMIA: A PROVEN ALTERNATIVE

SUPPLY & DEMAND

EZ Artemia is a liquid micro-capsulated diet formulated to mimic the nutritional value of enriched Artemia. Unlike live Artemia, which is subject to nutrient variability, EZ Artemia provides a consistent nutrient composition that is completely biosecure. With EZ Artemia, there is no extra preparation or cost involved from wild cysts…it is ready to feed right from the jug!

EZ ARTEMIA

Improved Survival

Biosecure

Consistent High HUFA Content

Contains VpakTM

Industry Standard

Extends Transport Time

Stable Supply

Consistent Nutrient Profi le

Complete Size Range

Contains Enzymes & Probiotics

No Storage & Preparation Costs

Stays in Suspension

001 02 03 04 05 06 07 08 09 10 11

1,000,000

2,000,000

3,000,000Lbs

Year

Artemia: Global Supply vs. Demand

4,000,000

5,000,000

6,000,000

World Supply – Dry ProcessedWorld Demand

“We have achieved 100% replacement of Artemia (nauplii) in production and transport…reduced ammonia levels and bacteria loads have improved water quality and animal health.” – Mexico

“A good amount of lipid is observed in the hepatopancreas…EZ Artemia gives us a good means to eliminate vibrio due to contamination from Artemia.” – Honduras

“EZ Artemia is an excellent product that reduces processing costs…it lowers the frequency of attacks of bioluminescence. – Panama

“We just conducted trials replacing live Artemia in PL transport…we intend to start using it in our larval rearing.” – Brazil

“EZ Artemia has demonstrated higher survivals and animals having well-marked digestive tracts…we have replaced 100%Artemia in 2011.” – Mexico

CUSTOMER FIELD RESULTS

ARTEMIA PRODUCT COMMENTS

EZ Artemia contains enzymes and probiotics to enhance digestion and improve water quality and animal health.

EZ Artemia is practically neutrally buoyant.

EZ Artemia does not require hatching and can reduce costs approximately $15/kg before the expense of cysts and enrichment.

EZ Artemia is formulated to match the nutrient profi le of very high quality or enriched Artemia.

Field tests have demonstrated higher survivals with EZ Artemia.

Artemia is a known vector for disease. EZ Artemia is pathogen free and biosecure.

VpakTM (Vitality Pak) enhances immune response and helps animals resist disease.

EZ Artemia can be fed from Zoea 1 to PL harvest; a smaller size particle size can be entirely consumed by fi lter feeders.

Due to heavy reliance on natural Artemia, it has become the recognized standard within the industry.

EZ Artemia maintains water quality, and unlike Artemia, reduces oxygen demand during PL transport.

World demand is exceeding the natural supply of Artemia impacting both pricing and availability.

Artemia nutrition varies based on the strain, harvest, and life stage.

Month 2

40 50 60 70 80 90 100

% Survival

Artemia vs. EZ Artemia – Shrimp PL Survival

Month 1

ArtemiaEZ Artemia

78.6% 11 Days to PL 4

89.2% 11 Days to PL 4

87.2% 13 Days to PL 4

88.7% 12 Days to PL 4

Enriched

FREQUENTLY ASKED QUESTIONS

ZEIGLER COMPLETE HATCHERY LINE

What percent of natural Artemia will it replace?In shrimp production trials, EZ Artemia demonstrated 100% replacement of Artemia and gave better survivals, larger PLs and faster growth. EZ Artemia consistently provides survivals of greater than 70%.

How do you feed it?For best results, feed the recommended amount of EZ Artemia every two hours. 1) Shake the jug well, 2) Measure into a beaker, 3) Dilute 1 part EZ Artemia with 10 parts water, 4) Swirl to mix well, and 5) Distribute evenly throughout the tank. Nothing could be EZer!

How do feeding rates of EZ Artemia and Artemia compare?1 kg of EZ Artemia replaces 1 kg of Artemia cysts (80% hatch-out) or the resulting 2-3 kg of wet weight Artemia derived from 1 kg of cysts. EZ Artemia is designed to replicate enriched Artemia and therefore has a superior nutrient profile and fatty acid (HUFA) composition than regular Artemia.

How does this product save the hatchery money?EZ Artemia does not require the extra personnel, tanks, pumps, blowers and freezer space associated with Artemia which can add up to $15/kg in additional costs. It also can cut up to 3 days off of the culture cycle, saving several hundred dollars/million PLs produced in cost savings. Finally, unlike Artemia, it is not subject to supply limitations and the associated price instabilities.

How can EZ Artemia help with biosecurity in a hatchery?Hatched Artemia is a known vector of many very pathogenic bacteria. Using EZ Artemia reduces the introduction of pathogens into your production tank because it is free of all known shrimp pathogens. VpakTM (Vitality Pak) is included in the formulation to help boost immune response and resist disease. Anti-microbial probiotics are included to inhibit pathogens.

Does this product need any special storage such as with Artemia?EZ Artemia has a shelf life of up to 2 years if kept in a cool location (22° C/77° F). It can be refrigerated, but not frozen. Open containers can be used up to 6 weeks.

Larval Esencial

Larval Z-Plus

Larval AP-100

Artemia Flakes

Shrimp Broodstock

Shrimp Maturation

EZ Black

Shrimp Starter

EZ MateMaturation & Broodstock

EZ LarvaLarval Feeds

PL Raceway PlusPost-Larval Feeds

VpakTM

BiopakTM

EZ BioHealth Enhancement

Pre-ground, Artemia flake to enhance monitoring of feeding and animal activity through coloration of the digestive tract

Cost-effective post-larval feed utilizing highly-digestible marine ingredients

Complete, premium diet to enhance growth and produce stronger PLs for acclimation into ponds

Well-proven larval feed formulated to support good health and survivals in fish and fresh water shrimp larvae

Cost-effective dry larval feed based utilizing marine ingredients and providing enhanced attractability

Premium dry larval feed utilizing high levels of HUFAs and cholesterol to promote molting and growth

Highly attractable flakes formulated with brine shrimp; Available in black and red colors providing enhanced pigmentation

Highly palatable diet formulated to reduce lag time associated ovarian maturation

Complete pelleted diet supporting transition from growout to Broodstock. Contains high levels of marine proteins & oils to reduce time to maturation

Pond probiotic or feed supplement designed to reduce organic sludge and improve water quality in ponds

Partial replacement of fresh feeds; contains high levels of HUFAs and astaxanthan. Proven to increase spawning frequency, as well as quantity and quality of nauplii

Probiotic designed to reduce vibrio and other pathogens in hatchery rearing tanks

Feed supplement to increase survivals during stressful conditions in ponds

Premium, micro-capsulated, liquid larval feed designed to improve water quality, shorten the culture cycle, and produce stronger PLs

Zeigler Bros., Inc. P.O. Box 95

Gardners, PA 17324 USA717-677-6181 phonewww.zeiglerfeed.com

Zeigler Aquaculture © 2012

A Proven Alternative to Natural Artemia

Micro-capsulated Liquid Larval DietEZZeigler Tradition

Setting aside their orchestra careers touring as The Scotch Highlanders, two brothers, Ty and LeRoy Zeigler, moved their young families to rural Gettysburg, Pennsylvania, where they had just purchased a farm and a water-powered grist feed mill. The year was 1935.

The first feeds were manufactured under the Conewago brand, named after the stream which powered their newly purchased mill. Their strong orientation to value, integrity and customer service was soon rewarded by the trust and business of many local farmers.

In 1967, the leadership passed to LeRoy’s son, Dr. Tom Zeigler, who transitioned the company from a manufacturer of farm feeds to an internationally recognized producer of specialized animal feeds. Beginning in the 1980s, the company began licensing nutritional and manufacturing technologies globally under its Zeigler brand.

The Zeigler family tradition continues today, more than 75 years later, under the leadership of Tom’s sons, Tim and Matt Zeigler. With a renewed spirit toward technology and innovation, the brothers continue to uphold the company traditions of value and integrity, while striving to serve the changing needs of loyal customers.

Circa 1950’s.

Worldwide demand for Artemia cysts continues to rise to levels which could exceed global supply (see below). In past years, good harvests and adequate inventories have helped Artemia suppliers meet market demands. Today, as global demand increases, the combination of depleted inventories and unpredictable harvests could result in supply shortfalls much sooner than anticipated.

It is important that the industry understands Artemia’s limitations and continues to focus its efforts to reduce the risks and costs of excessive reliance on this natural resource. Developing practical and cost-effective alternatives to Artemia will clearly help aquaculture become a more sustainable means to feed our planet.

“We have achieved 100% replacement of Artemia (nauplii) in production and transport…reduced ammonia levels and bacteria loads have improved water quality and animal health.” – Mexico

“A good amount of lipid is observed in the hepatopancreas…EZ Artemia gives us a good means to eliminate vibrio due to contamination from Artemia.” – Honduras

“EZ Artemia is an excellent product that reduces processing costs…it lowers the frequency of attacks of bioluminescence. – Panama

“We just conducted trials replacing live Artemia in PL transport…we intend to start using it in our larval rearing.” – Brazil

“EZ Artemia has demonstrated higher survivals and animals having well-marked digestive tracts…we have replaced 100% Artemia in 2011.” – Mexico

Zeigler Bros., Inc. is a pioneer and leader in the development and formulation of specialized feeds for the global aquaculture industry. Our commitment is to the worldwide growth of aquaculture through continued research and development. No matter the species or production system, we are experienced and equipped to provide the solutions you need to improve the health and productivity of your aquaculture operation.

EZ ARTEMIA: A PROvEN ALTERNATIvE

CUSTOMER FIELD RESULTS PRODUCT SPECIFICATIONS

FEEDING GUIDE & INSTRUCTIONS

SUPPLY & DEMAND

EZ Artemia is a liquid micro-capsulated diet formulated to mimic the nutritional value of enriched Artemia. Unlike live Artemia, which is subject to nutrient variability, EZ Artemia provides a consistent nutrient composition that is completely biosecure. With EZ Artemia, there is no extra preparation or cost involved from wild cysts…it is ready to feed right from the jug!

EZ ARTEMIA ARTEMIA PRODUCT COMMENTS

EZ Artemia contains enzymes and probiotics to enhance digestion and improve water quality and animal health.

Improved Survival

EZ Artemia is practically neutrally buoyant.

EZ Artemia does not require hatching and can reduce costs approximately $15/kg before the expense of cysts and enrichment.

EZ Artemia is formulated to match the nutrient profile of very high quality or enriched Artemia.

Field tests have demonstrated higher survivals with EZ Artemia.

Artemia is a known vector for disease. EZ Artemia is pathogen free and biosecure.Biosecure

Consistent High HUFA Content

Contains VpakTM VpakTM (Vitality Pak) enhances immune response and helps animals resist disease.

EZ Artemia can be fed from Zoea 1 to PL harvest; a smaller size particle size can be entirely consumed by filter feeders.

Industry Standard Due to heavy reliance on natural Artemia, it has become the recognized standard within the industry.

Extends Transport Time EZ Artemia maintains water quality, and unlike Artemia, reduces oxygen demand during PL transport.

Stable Supply World demand is exceeding the natural supply of Artemia impacting both pricing and availability.

Artemia nutrition varies based on the strain, harvest, and life stage.Consistent Nutrient Profile

Complete Size Range

Contains Enzymes & Probiotics

No Storage & Preparation Costs

Stays in Suspension

001 02 03 04 05 06 07 08 09 10 11

1,000,000

2,000,000

3,000,000Lbs

Year

Artemia: Global Supply vs. Demand

4,000,000

5,000,000

6,000,000

World Supply – Dry ProcessedWorld Demand

Month 2

40 50 60 70 80 90 100

% Survival

Artemia vs. EZ Artemia – Shrimp PL Survival

Month 1

ArtemiaEZ Artemia

78.6% 11 Days to PL 4

89.2% 11 Days to PL 4

87.2% 13 Days to PL 4

88.7% 12 Days to PL 4

Particle Size – 50-200 microns and 300-500 microns

Guaranteed Analysis

Dry Weight Wet WeightCrude Protein Minimum 52.0% 14.0%Crude Fat Minimum 17.0% 4.5%Crude Fiber Maximum 3.7% 1.0%Moisture Maximum 0.0% 73.0%

Packaging – Jugs: 1 kg (2.2 lb), 2 kg (4.4 lb) and 10 kg (22 lb)

Product Storage

Best if used within twenty-four (24) months from date of manufacture.•

Can be refrigerated – do not freeze. •

Opened jugs should be used within six (6) weeks.•

Store sealed jugs in a cool (22° C/72° F), dry place away from sunlight. •

Shake well prior to each use. •

Keep capped after each use.•

Rotate stock to use oldest product first (“first in, first out” principle).•

Sold pursuant to license from Cargill, Incorporated under U.S. Patent No. 5,698,246

EZ Artemia Feeding Protocol per Million Larvae

*For best results, feed every two hours.

Unlike Artemia, which requires hatching, EZ Artemia is very easy to use.

1. Shake Well Do Not Freeze Do Not Blend Do Not Screen4. Swirl2. Measure 5. Distribute Evenly Throughout

Tank

3. Dilute 1 partEZ Artemia with10 parts water

Z1 Z2 Z2 Z3 M1 M2 M3 PL1 PL2 PL3 PL4 PL5 PL6 PL7 PL8 PL9 PL10 PL11 PL12 PL13 PL14 PL15 Total (kgs)

27 36 72 90 105 150 210 203 270 1.16

203 270 525 638 615 165 188 221 246 258 278 293 308 323 338 4.87

StageEZ Artemia 50-200 (mls/day per million)

EZ Artemia 300-500 (mls/day per million)

Enriched

Zeigler Bros., Inc.P.O. Box 95

Gardners, PA 17324 USA717-677-6181 phonewww.zeiglerfeed.com

Zeigler Aquaculture © 2012

Zeigler TraditionSetting aside their orchestra careers touring asThe Scotch Highlanders, two brothers, Ty and LeRoy Zeigler, moved their young families torural Gettysburg, Pennsylvania, where they hadjust purchased a farm and a water-powered grist feed mill. The year was 1935.

The fi rst feeds were manufactured under the Conewago brand, named after the stream which powered their newly purchased mill. Their strong orientation to value, integrity and customer service was soon rewardedby the trust and business of many local farmers.

In 1967, the leadership passed to LeRoy’s son, Dr. Tom Zeigler, who transitioned the company from a manufacturer of farm feeds to an internationally recognized producer of specialized animal feeds.Beginning in the 1980s, the company began licensing nutritionaland manufacturing technologies globally under its Zeigler brand.

The Zeigler family tradition continues today, more than 75 years later, under the leadership of Tom’s sons, Tim and Matt Zeigler. With a renewed spirit toward technology and innovation, the brothers continue to uphold the company traditions of value and integrity, while striving to serve the changing needs of loyal customers.

Circa 1950’s.