xGen® Lockdown® products for next generation sequencing

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xGen® Lockdown® Probes are individually synthesized nucleic acids for target enrichment• In-solution hybridization probes• 120-nucleotide DNA oligos with a 5′ biotin modification• Synthesized using IDT’s proprietary Ultramer® synthesis process to ensure

more full-length product• Individual synthesis offers more flexibility than array synthesis offered by

other manufacturers

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• Filter out unwanted sequences• Focus only on the regions of interest• Increase depth of coverage

Begin with prepared library (using e.g., Illumina TruSeq® HT kit or Ion Torrent®

library prep kit).

A simple target capture workflow facilitates focused sequencing

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Various formats of xGen® Lockdown® Probes to suit individual sequencing needs• Design fully customized panels

– xGen Lockdown Probes• Create panels from predesigned probe pools

– xGen Predesigned Gene Capture Pools

• Select from stocked, validated panels:– xGen Exome Research Panel– xGen AML Panel– xGen Pan-Cancer Panel– xGen Inherited Diseases Panel

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xGen® Lockdown® Probes allow cost-effective target enrichment using fully custom panels

Size Scale (pmol) Reactions/probe

Mini 3 1

Standard 24 8

XL 192 64

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1. Visit www.idtdna.com/xGen.2. Select “Learn more about xGen Lockdown Probes”.3. Click “Create Design”.

Easy ordering with an intuitive online tool

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In a single, streamlined transaction, order xGen Lockdown Reagents, hybridization and wash buffers optimized for xGen Lockdown Probes.

Ordering tool streamlines the process for purchasing required reagents

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• Illumina• Ion Torrent• Custom

Select specific adapter blockers for the appropriate sequencing platform or design custom blockers

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What are xGen® Predesigned Gene Capture Pools?

• Versatile pools of xGen® Lockdown® Probes designed to target specific genes

• Allow fast and easy custom panel design• Delivered combined in a single tube • Available for 16 or 96 reactions• Shipped in solution and ready to use

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Features of xGen® Predesigned Capture Pools

• Target coding regions (CDS) of human RefSeq genes• Designed with repeat masking and 1X tiling• Flat price:

– US$100/gene for 16 reactions– US$150/gene for 96 reactions

• Ship in 7–10 days

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xGen® Lockdown® Panels allow efficient targeted sequencing for specialized areas of research

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Inventoried panels No. of gene targets

Span of human genome (Mb) Shipment

Acute Myeloid Leukemia 264 1.2 Dried down

Pan-Cancer 127 0.8 Dried down

Inherited Diseases 4,503 12 Dried down

Exome Research 19,396 39 In solution

• Uniformity of coverage is a requirement to ensure reliable sequencing data from target capture

• Deep coverage with high uniformity provides greater confidence in calling rare events

• High uniformity allows multiplexing of more samples because sequencing reads do not have to be sacrificed to overcome poor uniformity

High coverage uniformity of xGen® Lockdown® Panels increases multiplexing capacity and reduces cost

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• Consistent results from multiple experiments– 9 libraries sequenced in pools of 3

show little variation in overall coverage• Confidence in calling germline mutations

– >98% of target bases covered at 20X– >96% of target bases covered at 30X

• Excellent depth of coverage achieved without sacrificing sequencing reads– 50M reads per sample

The xGen® Exome Research Panel provides consistent, deep coverage over targeted regions

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100 million reads

xGen® Exome Research Panel provides deeper coverage than array-synthesized probes from 2 different sources

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40 million reads

Advantages of xGen® Lockdown®

Probes

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• High uniformity allows capture of regions of high or low GC content

• Uniformity is a product of:– Individual probe synthesis using

IDT proprietary platform– Individual mass spec

measurement– Individual OD measurement

xGen® Lockdown® Probes provide more uniform coverage than array-synthesized probes

16[Data courtesy of Genoptix, Inc.]

xGen® Lockdown® Probes and Panels allow complete flexibility in panel composition• Supplement panels with additional xGen Lockdown Probes to capture

more targets• Combine panels to meet changing project needs

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Custom panels designed using xGen® Lockdown® Probes are shipped within 2 weeks

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IDT turnaround time

Competitors’ turnaround time

Target capture can be completed in 1 day with xGen®

Lockdown® Probes and Reagents

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Questions?

www.idtdna.com/xGen

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www.idtdna.com/scitools.

• qPCR and PCR amplification

• Gene regulation and knockdown

• MicroRNA Analysis

• Gene design

• Sequencing

• Oligo dilution and resuspension

Our intuitive analysis and design tools and calculators are available for:

Begin designing at

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