workpackage 02 germination testing christoph germeier, zofia bulinska – radomska, izabela...
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Workpackage 02Germination testing
Christoph Germeier,Zofia Bulinska – Radomska, Izabela Borowska, Audrey Didier
AGRI GENRES 061: Avena genetic resources for quality in human consumption (AVEQ), 02-04 March, 2009, Quedlinburg
Germination methodology check test
Germination test results 2008
Germination test results 2009
Germination methodology
Germination and Fusarium counting methodology
germination test: how many accessions are being tested, when the tests have been started, how long do they all take?
AGRI GENRES 061: Avena genetic resources for quality in human consumption (AVEQ), 02-04 March, 2009, Quedlinburg
Germination methodology
General outline of germination tests according to ISTA methodology:
Between paper in climate chamber, Continuous light 20°C, 4 replications (recommended 8) a 50 seedsBreaking dormancy with gibberellic acid: GA3 (1000 mg/l) in phosphate buffer (1.7799 g Na2HPO4 x 2 H20 + 1.3799 g NaH2PO4 x H2O + 1 l aqua dest.)Count after 5 and 10 days
Variants used in the project
FRA 2007 / 2008: Round filter paper (diameter: 90mm) are moistened with gibberellin solution and placed in the petri plate then seeds are laid down onto the filter paper. A second moistened round filter paper is disposed on the top.
POL 2007 / 2008: Filter paper envelopes (CHEMPUR, 65g/m2, 12,5x18,5 cm) are moistened with gibberellin solution. Seeds are placed into the envelope, incubated at 200C with continuous light.
AGRI GENRES 061: Avena genetic resources for quality in human consumption (AVEQ), 02-04 March, 2009, Quedlinburg
Germination methodology: Radzików
AGRI GENRES 061: Avena genetic resources for quality in human consumption (AVEQ), 02-04 March, 2009, Quedlinburg
Mold
Germination methodology: Radzików
Normal germ
AGRI GENRES 061: Avena genetic resources for quality in human consumption (AVEQ), 02-04 March, 2009, Quedlinburg
Germination methodology: Radzików
Normal germ
Fusarium
AGRI GENRES 061: Avena genetic resources for quality in human consumption (AVEQ), 02-04 March, 2009, Quedlinburg
DEU 2007 / 2008: Filter paper towels (e.g. Munktell C160, 15x60cm2) are pre-folded and moistened with gibberellin solution. Seeds are placed onto the fold, the towel then folded, rolled, stabilized in a plastic bag and incubated at 200C with continuous light.
2007: climate cabinetside of plastic bag open
2008: climate chamber top of plastic bag open
AGRI GENRES 061: Avena genetic resources for quality in human consumption (AVEQ), 02-04 March, 2009, Quedlinburg
Germination methodology: Quedlinburg
Set up of germination tests in climate chamber (2008)
AGRI GENRES 061: Avena genetic resources for quality in human consumption (AVEQ), 02-04 March, 2009, Quedlinburg
Germination and Fusarium counting methodology: Quedlinburg
A) Abnormal germ and Molds
AGRI GENRES 061: Avena genetic resources for quality in human consumption (AVEQ), 02-04 March, 2009, Quedlinburg
Germination and Fusarium counting methodology: Quedlinburg
B) Fusarium
more or less thick mycelium, reddish color, plants affected or not germinated
AGRI GENRES 061: Avena genetic resources for quality in human consumption (AVEQ), 02-04 March, 2009, Quedlinburg
Germination and Fusarium counting methodology: Quedlinburg
B) Fusarium
more or less mycelium, reddish colour, plants not affected
AGRI GENRES 061: Avena genetic resources for quality in human consumption (AVEQ), 02-04 March, 2009, Quedlinburg
Germination and Fusarium counting methodology: Quedlinburg
B) Fusarium
mainly reddish color, mycelium, symptoms not detectable
AGRI GENRES 061: Avena genetic resources for quality in human consumption (AVEQ), 02-04 March, 2009, Quedlinburg
Germination methodology check test (wild species)
Accession FRA POLDEU bag
closed
DEUside open
DEUtop
open
A. barbata ssp. barbata
PRT 1984 DEU146 AVE 850 75 89,5 69 80 78
A. clauda BGR001 A2E0003 52 50,5 5 62 65
A. damascena POL003 PL52345 32,5 97,5 60,5 94,5
A. fatua AUT 1986 DEU146 AVE 2671 47,5 79,5 65,5 83,5 82
A. fatua ssp. fatua ROM DEU146 AVE 2804 63 81,5 36,5 88 81,5
A. sterilis ssp. ludoviciana
ALB 1994 DEU146 AVE 3066 77,5 71,5 20 86
AGRI GENRES 061: Avena genetic resources for quality in human consumption (AVEQ), 02-04 March, 2009, Quedlinburg
Accession FRA POL DEU bagclosed
DEUside open
DEUtop open
A. abyssinica 1939 BGR001 BGR 8133 25 54,5 25 35 44
A. abyssinica cult. Finca-san-jose
ESP DEU146 AVE 4711 78 78 60,5 80 82
A. strigosa var. nigra BEL 1998 RUS001 200114678 25,5 87,5 61,5 74
A. strigosa var. typica Local
NOR 1978 RUS001 200112562 73,5 80 17 79 77
A. strigosa ssp. brevis var. nudibrevis
ESP 1989 POL003 PL51277 60,5 88 64,5 84
A. strigosa ssp. brevis var. nudibrevis cult. Saia
DEU 1959 DEU146 AVE 436 13,5 78 24 67,5 80,5
Germination methodology check test (marginally cultivated species)
AGRI GENRES 061: Avena genetic resources for quality in human consumption (AVEQ), 02-04 March, 2009, Quedlinburg
Germination methodology check test
Accession FRA POL DEU bag
closed
DEUside open
DEUtop
open
A. byzantina ssp. byzantina cult. Zutna kisa
HRV 1996 DEU146 AVE 3095 29,5 76 11 77,5
A. byzantina ssp. byzantina var. aurea
AUT DEU146 AVE 2741 45 73 49,5 76,5 81
A. sativa var. nigra cult. Stormogul Ii
SWE 1918 SWE002 NGB4866 82 95,5 69 92,5
A. sativa ssp. nudisativa var. chinensis cult. Platek
POL 1974 POL003 PL50731 42,5 86,5 28,5 88,5
A. sativa ssp. nudisativa var. chinensis Cult. Weißer Nackter Fahnen
DEU DEU146 AVE 674 31 70 35 65,5 77,5
Standard cultivars from breeder
A. sativa Cult. Aintree FRA 1992 FRA261 AINTREE 65,5 99,5 85 98 98
A. sativa Cult. SW Argyle GBR SWE006 SW Argyle 78 94,5 79 94,5
A. sativa Cult. Zuton GBR GBR016 Zuton 87 85,5 59,5 80,5 86
Germination methodology check test (cultivated oat)
AGRI GENRES 061: Avena genetic resources for quality in human consumption (AVEQ), 02-04 March, 2009, Quedlinburg
Germination tests total summary
Year
Lab
Tests/ D
ays
To
tal Accessio
ns
Avg
Accessio
ns p
er test (d
ay)
Germ
inab
ility
Infestatio
n
Fu
sarium
2008 DEU 16 385 24,1 54,9 57,2 3,1
FRA 10 374 37,4 50,2 0,1 1,9
POL 8 211 26,4 81,1 13,8 6,7
2009 DEU 10 449 44,9 68,8 45 3,1
FRA 7 300 42,9 58,5 0 0
POL 16 320 20 80,8 14,5 6,3
AGRI GENRES 061: Avena genetic resources for quality in human consumption (AVEQ), 02-04 March, 2009, Quedlinburg
Germination results species wise – wild speciesDEU FRA POL
Species Year N
Min Avg
Max N
Min Avg
Max N
Min Avg
Max
A. barbata 2007 3 68 79 90 1 57 0
2008 3 69 82 96 2 43 50 58 0
A. brevis 2008 2 74 74 74 2 84 84 84 0
A. canariensis 2007 1 46 0 0
2008 1 80 1 42 0
A. clauda 2007 1 65 0 0
A. damascena 2007 0 1 46 2 100 100 100
A. fatua 2007 8 49 74 88 9 9 56 76 0
2008 11 55 71 88 7 21 49 73 0
A. hirtula 2007 1 59 0 0
2008 3 70 83 92 0 0
A. hybrida 2007 1 74 1 83 0
A. sterilis 2007 4 56 74 86 0 0
A. sterilis 2008 4 18 64 88 3 1 31 62 0
A. wiestii 2007 1 63 0 0
AGRI GENRES 061: Avena genetic resources for quality in human consumption (AVEQ), 02-04 March, 2009, Quedlinburg
Germination results species wise
DEU FRA POL
Species Year N
Min A
vg
Max N
Min A
vg
Max N
Min A
vg
Max
Marginally cultivated species
A. abyssinica 2007 3 40 59 82 3 30 49 82 0
A.abyssinica 2008 5 50 61 74 3 32 39 52 2 78 83 88
A. strigosa 2007 21 21 51 80 21 20 62 88 5 50 80 92
A. strigosa 2008 39 49 69 90 32 34 62 90 29 0 77 97
Cultivated oats
A. byzantina 2007 16 37 60 81 16 14 48 82 6 62 75 84
A. byzantina 2008 25 50 63 82 22 16 55 82 24 71 85 96
A. sativa 2007 261 10 57 90 257 2 49 97 173 6 80 100
A. sativa 2008 295 28 67 92 263 0 56 98 263 0 80 100
AGRI GENRES 061: Avena genetic resources for quality in human consumption (AVEQ), 02-04 March, 2009, Quedlinburg
Fusarium [%] results species wise – wild speciesDEU FRA POL
Species Year N
Min A
vg
Max N
Min A
vg
Max N
Min A
vg
Max
A. barbata 2007 3 0,5 3,5 6,5 1 2,04 2,04 0
2008 3 1,5 3 5,5 2 0 0 0
A. brevis 2008 2 0,5 0,75 1 2 0 0 0
A. canariensis 2007 1 2 2 2 0 0
2008 1 2 2 2 1 0 0 0
A. clauda 2007 1 3 3 3 0 0
A. damascena 2007 0 1 3,5 0,54 0,54 2 3,5 4 4,5
A. fatua 2007 8 0,5 2,5 6 9 0,06 0,5 0
2008 11 0,5 2,41 4 7 0 0 0
A. hirtula 2007 1 0,5 0,5 0,5 0 0
2008 3 0 0,83 2 0 0
A. hybrida 2007 1 0,5 0,5 0,5 1 0 0 0
A. sterilis 2007 4 0,5 2,62 6 0 0
A. sterilis 2008 4 1 2,62 6 3 0 0 0
A. wiestii 2007 1 1 1 1 0 0
AGRI GENRES 061: Avena genetic resources for quality in human consumption (AVEQ), 02-04 March, 2009, Quedlinburg
Fusarium results [%] species wise – cultivated species
DEU FRA POL
Species Year N
Min
Avg
Max N
Min
Avg
Max N
Min
Avg
Max
Marginally cultivated species
A. abyssinica 2007 3 3,5 4,17 5 3 2,33 6,5 0
A. abyssinica 2008 5 0,5 2,7 6,5 3 1 0 0 2 1 4,25 7,5
A. strigosa 2007 21 0,5 2,36 6 21 0,5 0,62 4,53 5 0,5 7,8 17,5
A. strigosa 2008 39 0,5 2,32 5,5 32 0 0 0 29 0 2,17 8
Cultivated oats
A. byzantina 2007 16 1 3,47 6,5 16 3 1,78 6 6 3 9,17 15,5
A. byzantina 2008 25 1 3,88 8 22 0,5 0 0 24 0,5 6,9 18
A. sativa 2007 261 0 3,39 11 257 1 2,49 20,59 173 1 7,5 28
A. sativa 2008 295 0 3,7 13 263 0 0 0 263 0 6,8 26
AGRI GENRES 061: Avena genetic resources for quality in human consumption (AVEQ), 02-04 March, 2009, Quedlinburg
Germination check results compared with germination test 2007/2008
Species AccessionGerminability (2007/2008)
FRACheck
POLCheck
DEUCheckSide open
DEU CheckTop open
barbata DEU146 AVE 850 74,5 (DEU) 75 89,5 80 78
clauda BGR001 A2E0003 19 (DEU) 52 50,5 62 65
damascena POL003 PL52345 100 (POL) 32,5 97,5 94,5
fatua DEU146 AVE 2671 80 (DEU) 47,5 79,5 83,5 82
fatua DEU146 AVE 2804 52 (DEU) 63 81,5 88 81,5
sterilis DEU146 AVE 3066 69,5 (DEU) 77,5 71,5 86
AGRI GENRES 061: Avena genetic resources for quality in human consumption (AVEQ), 02-04 March, 2009, Quedlinburg
Germination check results compared with germination test 2007/2008
Species AccessionGerminability (2007/2008)
FRACheck
POLCheck
DEUCheckSide open
DEU CheckTop open
Marginally cultivated species
abyssinica BGR001 BGR 8133 48 (DEU) 25 54,5 35 44
abyssinica DEU146 AVE 4711 57 (DEU) 78 78 80 82
strigosa RUS001 200112562 13 (DEU) 73,5 80 79 77
strigosa POL003 PL51277 84 (POL) 60,5 88 84
strigosa DEU146 AVE 436 24 (DEU) 13,5 78 67,5 80,5
Cultivated oats
byzantina DEU146 AVE 3095 84 (POL) 29,5 76 77,5
byzantina DEU146 AVE 2741 28 (DEU) 45 73 76,5 81
sativa SWE002 NGB4866 98 (POL) 82 95,5 92,5
sativa POL003 PL50731 91 (POL) 42,5 86,5 88,5
sativa DEU146 AVE 674 29,5 (DEU) 31 70 65,5 77,5
AGRI GENRES 061: Avena genetic resources for quality in human consumption (AVEQ), 02-04 March, 2009, Quedlinburg
ConclusionMain problem reducing germinability:Excess of water!
AGRI GENRES 061: Avena genetic resources for quality in human consumption (AVEQ), 02-04 March, 2009, Quedlinburg
Thanks for your attention