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    Learning Experience 3

    No Mat te r Wha tYou r Shape

    The easiest traits to describe in an organism are the visible traits. For instance,think about distinguishing between two people. You might use the texture of theirhair as an easily identifiable trait. One might have curly hair, and the otherstraight hair. By using differences in hair texture, you have described variantsof a single trait. In this case, that trait is hair texture.

    What is responsible for variants in traits? Are traits and their variants onlycharacteristics that are directly observable? Or are there underlying causes forthese variants? In this learning experience, you use variations in the shape ofpeas as a simple model for investigating the answers to these questions. You then explore a trait in humans, sickle-cell trait, as another example of variations in traits.

    Your teacher will distribute two different kinds of peas to you and your partner.Examine them carefully. Discuss the following questions with your partner, andrecord your thinking in your notebook. Be prepared to discuss your ideas withthe class.1. List the traits you can observe in each kind of pea.2. Describe the variations, if any, in each trait you have listed for the two kinds

    of peas.3. What do you think might cause the variations in these traits?4. What if you were to soak these peas in water? Do you think there would be a

    difference in the amount of water they absorbed? Explain your answer.



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  • A Pea by Any Other Name Is Still a SeedHow might you begin to answer the question, Why is one pea wrinkled andanother round? Think about ways you could find out more about the differencesbetween round and wrinkled peas. Are there differences in how the peas areconstructed? How might these differences produce a wrinkled shape instead of around shape? Are there differences in what the seed is made of? How would thisaffect the shape?

    In this learning experience, you will investigate the causes of variations intraits of organisms. Using these pea variants as a model, you will look at thedifferences between wrinkled peas and round peas (which are actually seeds).But first, you need to understand the general structure of a seed(shown in Figure 2.7). A seed is the part of a plant that resultsfrom the fertilization of the female egg by the male pollen.Following fertilization, the embryonic plant develops within aprotective seed coat. In addition to the embryonic plant and theseed coat, the seed also contains a source of food. The germinatingseedling will use this food until it can carry out photosynthesis. Inone type of plant, this food source is in a separate structure withinthe seed called the endosperm. In another type of plant, theprotein, starch, and fats are stored in two large seed leaves.Regardless of the system of storage, the newly sprouted plantdepends on these stored food sources until it can make its own.

    Seeds have a very low water content. During the final stages ofthe development of the seed, cells within the seed dehydrate. Inother words, most of the water in the seed is removed. The resultinglow water content in the seed causes most cellular processes to slowdown or stop. In this dehydrated state, the embryonic plant canremain dormant (inactive) but viable (alive) within the seed. It can stay this wayfor long periods of time without growing or developing. This process enables theseed to delay germination until environmental conditions are suitable for itsgrowth. Germination is the start of growth and development of a plant. This iswhen the embryonic plant breaks out of its seed coat. When conditions becomefavorable, water enters the seed. Rehydration triggers the reactivation of normalmetabolic processes, and germination begins (see Figure 2.8).

    As the plant begins to develop, it uses the starch stored in the endosperm orseed leaves as an energy source. A plant can use photosynthesis to provide itsrequired food and energy only after breaking free of the soil and receiving sunlight.

    In this activity, you will determine why some seeds (peas) are wrinkled at theend of their development and some are round.

    Learning Experience 3 No Matter What Your Shape 165


    Figure 2.7Structure of a seed.

    seed coat


    embryonic stem

    embryonic root

    seed leaves(cotyledon)

    embryonic shoot tip

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  • For each pair of students:

    2 pairs of safety goggles 10 round peas 10 wrinkled peas 1 balance 2 small beakers or containers (50-mL) 1 wax marking pencil 2 microscope slides with coverslips access to a compound microscope 1 razor blade or scalpel 1 forceps 1 dropping bottle of dilute Lugols iodine paper towels distilled water

    Part A1. Develop a hypothesis that explains why some peas are wrinkled

    and some are round. Record your hypothesis in your notebook.2. Read steps 38. Then create a data chart in your notebook. Fill it in as you

    carry out this part of the experiment.3. Weigh all 10 round peas together. Record the weight of the peas on your

    data chart. Weigh all 10 wrinkled peas together. Record the weight of thepeas on your data chart.

    STOP &T H I N K



    166 Learning Experience 3 No Matter What Your Shape





    (a) seed development

    (b) germination

    Figure 2.8(a) During seed development, water is lost from cells. This dehydration slows down metabolic processes. (b) Duringgermination, water enters the seed and the metabolic processes are reactivated. As a result, the embryonic plant begins to grow.


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  • 4. Use a wax marking pencil to label one beaker R and the other beaker W.Also mark the beakers with your group name. Place the dried round peas inthe R beaker and the dried wrinkled peas in the W beaker. Add water untilthe beakers are three-quarters full (see Figure 2.9).

    5. Place the beakers in the location designated by your teacher until the nextclass session.

    6. After soaking the peas overnight, retrieve your 2 beakers. Label one papertowel R and another W. Pour off the excess water from each beakercarefully. Then empty the peas from each beaker in a pile on theappropriately labeled paper towel.

    7. Weigh each pile of peas again to determine the weight after soaking. Recordthe weights in your table.

    8. Determine the weight difference for each kind of pea. Calculate the percentageof increase in weight for each kind of pea. Record the results in your table.

    9. Think about this experiment and your understanding of seed formation. Do you want to change your hypothesis as to why some

    peas wrinkle and others remain round when they are dried? Record yourresponse in your notebook.

    Part B1. Use a wax marking pencil to label one microscope slide R and another W.2. Place 1 drop of dilute iodine on each slide.3. Hold a soaked wrinkled pea with forceps. Cut the pea in half with a scalpel

    or razor blade. Cut a very thin segment or slice from the inside of the pea.Gently place the slice into the drop of dilute iodine on the slide labeled W(see Figure 2.10).

    4. Carefully wipe the blade of the scalpel or razor blade clean with a papertowel. Repeat step 3 with a soaked round pea. Drop the slice on the slidelabeled R.

    5. Place a coverslip at an angle over each drop and gently lower it. Observeeach slice under the microscope.

    6. Describe and draw in your notebook what you see. Compare the shapes, colors, patterns, and densities of the starch grains. Look at a

    few slides prepared by other class members and compare them with yours.

    STOP &T H I N K

    STOP &T H I N K

    Learning Experience 3 No Matter What Your Shape 167


    Avoid staining your

    skin with iodine. Ifiodine is accidentallyingested, seekimmediate medicalattention.

    Figure 2.9Lab setup.

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  • Prepare a laboratory report for this experiment in your notebook. Be sure toinclude the following:a. your initial hypothesis (step 1 in the procedure);b. your revised hypothesis (step 9), if you changed it (if you changed it,

    explain why);c. your experimental procedure;d. the purpose of each part of the experiment;e. your data (show all your calculations where appropriate); andf. any conclusions you can draw, based on your data.

    Decide whether you have enough data and knowledge to identify the cause of theshape difference between the two different kinds of peas. Explain your answer.

    Adding a New Wrinkle to the PictureYou have gathered a great deal of information about the differences betweenwrinkled and round peas. You know some things about these peas at both thevisible and the biochemical levels. But you still may not be able to reach aconclusion about the exact cause of the difference in shape. To identify the exactcause of this difference, you need to understand more about the biochemistry ofseed development.


    168 Learning Experience 3 No Matter What Your Shape


    a pea

    pea slicedrop of iodine


    Figure 2.10Hold the pea firmly with theforceps. Carefully slice severalsections (a) until a very thin section is obtained.Using the forceps, transferthe section to the drop ofiodine on the microscopeslide (b).


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  • In a developing seed, sucrose (a sugar) is converted to a highly branched form ofstarch. This starch is called amylopectin. It serves as a source of food for the developingplant. The conversion from sucrose to starch is facilitated by an enzyme. This enzyme iscalled starch-branching enzyme I (SBEI). Scientists used methods similar to the onesyou carried out in class. They were able to demonstrate that the characteristic ofwrinkled shape was the result of the peas inability to synthesize amylopectin fromsucrose. As a result of a faulty SBEI enzyme, these peas cannot make amylopectin. Theunchanged sucrose concentrates in the developing seed. Using a different enzyme,wrinkled peas can synthesize a different, unbranched form of starch. This form of starchis called amylose. Amylose serves as the seeds food source.

    What does this inability to make branched starch from sucrose have to dowith shape? During the seeds development, the high concentration of sucrosecaused water to accumulate inside the seed. The water, as in a water balloon,stretched the seed coat much more than it normally would stretch without theconcentrated sucrose. During the final stages of seed development, dehydrationtakes place. The accumulated water is lost from the seed. This causes thestretched seed coat to collapse, somewhat creating a wrinkled pea. In the peawith the functional SBEI enzyme, sucrose did not accumulate. Thus, the seedcoat was not stretched by excess water. So when the water was lost duringdehydration, the seed coat remained round (see Figure 2.11).

    Do round peas have only functional SBEI? Do wrinkled peas have onlynonfunctional SBEI? Would a pea having both kinds of enzymes be a little bitwrinkled? Biochemical analysis demonstrates that peas that have both kinds ofenzymes still appear round. You cannot see any difference from those peas thathave only functional SBEI. Some of the enzyme cannot make amylopectin fromsucrose in these seeds. Even so, the enzyme that can function can convertenough sucrose to amylopectin to prevent water from being retained andstretching the seed coat. They therefore appear round.

    Learning Experience 3 No Matter What Your Shape 169







    early seed development starch formation dehydration

    round peas


    1 2 3 Figure 2.11Sucrose changes to starch in seed development: Acomparison of round andwrinkled peas. (1) Sucrose ismade during early seeddevelopment. (2) In roundseeds, SBEI converts sucrose toamylopectin (branched starch).In wrinkled peas, the SBEI doesnot function correctly. So noamylopectin is made. Instead, a different enzyme converts some sucrose to amylose(unbranched starch). The highconcentration of unconvertedsucrose in the wrinkled seedcauses the seed to take up waterand swell. This stretches theseed coat. (3) When the seedundergoes dehydration, thecoat of the round seed remainssmooth. But the coat of thewrinkled seed wrinkles becauseof its stretched seed coat.

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    Think about an enzyme that can function efficiently even in the presence of anonfunctional or dysfunctional enzyme of the same kind. Its activity isconsidered dominant to the activity of the nonfunctional (or recessive) enzyme.An organism that has both kinds of enzymes and displays the trait of thedominant activity (in this case, round) is considered heterozygous for that trait.An organism with only one kind of enzyme is said to be homozygous. Awrinkled pea is always homozygous for the nonfunctional enzyme. But a roundpea could be either homozygous or heterozygous. In this case, you cannotnecessarily judge a pea by its cover.

    Am I a Carrier, and What Does That Mean?It was Health Day at Denzel Joness high school. Career Day, Health Day,Environment Day, fumed Denzel. When am I going to hear about stuff thatmatters to me? Denzels class filed into the auditorium. The air was buzzing withconversation about music, friends, the last biology exameverything except thetopic of health. Who cared, anyway? Well, at least it got them out of fifth period.

    As several individuals from the local health clinic talked, Denzel foundhimself drawn in by some topics. These included exercise, smoking, and methodsfor the prevention of infectious diseases. One topic in particular caught hisattention because he actually knew a couple of people with the problem. A

    physicians assistant began to talk about somethingcalled hemoglobinopathies. He described one inparticular, sickle-cell anemia. Denzels uncle Jamal(his fathers brother) had the disorder. He sufferedfrom fatigue and bouts of intense joint pain. Becauseit bothered him to watch his uncle suffer, Denzel wascurious about the cause.

    Denzel learned that sickle-cell anemia is a disorderof red blood cells that can run in families. It causesthe red blood cells to collapse into shapes resemblingsickles (see Figure 2.12). This happens when theoxygen level of the blood is low.

    Red blood cells sickle because they containhemoglobin that is biochemically a little differentfrom the normal hemoglobin protein. Normalhemoglobin (or hemoglobin A) is found in solution in

    red blood cells. It binds oxygen and transports it throughout the body. Once itreleases the oxygen, the hemoglobin remains in solution in the red blood cell.Sickling hemoglobin (designated S) is a variant form of hemoglobin. It differsfrom normal hemoglobin by only a single amino acid. That slight difference instructure, however, alters its function. Hemoglobin S binds oxygen and carries itto where it is needed. But a problem arises when the oxygen is released and theconcentration of oxygen around the h...