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WaterRF Project 4379 Research Needs for

Opportunistic Pathogens in Premise Plumbing

Kick-Off Conference Call September 6, 2011

1:00 PM EDT

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Agenda• Project organization, deliverables, and schedule• Participant commitments• Outline and approach to literature review• Overview of Wiki Site, Google docs and Zotero• Schedule and deadlines• Questions?

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Track 1: MethodologyTeam Leader – Amy

Pruden

Track 2b: Applied Microbial Ecology

Team Leader – Marc Edwards

Track 3: EpidemiologyTeam Leader –Joe

Falkinham

Track 1 TeamNorm Pace

Anne CamperGary BurlingameAaron RosenblattRhonda Birdnow

Student:Hong Wang

Track 2b TeamSusan Springthorpe

Michele PrevostYan Zhang

Paul BishopMarsha Pryor

Student:Sheldon Masters

Track 3 TeamBetsy Hilborn

Joan RoseJanet Stout

Jennifer ClancyMatt Arduino

Student:Lindsay Hamilton

Project Advisory Committee (PAC)Marsha Pryor

Marilyn MarshallMaggie RodgersAndrew Whelton

Project ManagersAmy Pruden

Randi LiebermanWaterRF Research Manager

Mary Smith

Track 2a: Microbial Ecology FundamentalsTeam Leaders– Edwards

and Pruden

Track 2a TeamNick Ashbolt

Vince HillMark LeChevallierLutgarde Raskin

Students:William RhoadsJordan Bird

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Overall Deliverables• Literature review synthesizing state of knowledge

of opportunistic pathogens in premise plumbing and identifying critical research needso Sept. 2011-Feb., 2012

• Expert Workshop to finalize literature reviewo Monday March 26 Tuesday March 27, 2012

• Prioritized listing of project descriptions for consideration for future RFPs.

• Disseminationo Multi-author peer-review journal article(s)o Trade journal article (e.g., Opflow)o Webinaro ACE-12 Dallas, June 2012o Final Report

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Participant Commitments

• Participation in three Track conference calls to aid in the development of the literature review

• Write an assigned section of the literature review

• Read the synthesized literature review prior to the Workshop

• Participate in the Workshopo Assess and finalize literature reviewo Identify and rank research needso Assist in writing tentative RFPso Enjoy the Workshop!

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Pathogens of ConcernPathogen Disease(s)

Host Organism Required?

Mode of Exposure

Legionella pneumophila

Legionnaires’ disease orPontiac fever in children Yes Inhalation or

aspiration

Pseudomonas aeruginosa

  

Urinary tract infections, respiratory infections, dermatitis, soft tissue infections, bacteremia,

bone and joint infections, GI infections

NoWound infection; other modes of

transmission are unknown

Mycobacterium avium

Pulmonary disease,cervical lymphadenitis (children) No Inhalation or

aspiration

Acanthamoeba Acanthamoeba keratitis No Wound infection

Naegleria fowleri Primary amebic meningoencephalitis No Nasal aspiration

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Focus on Potable Water!

• Other environments (e.g., cooling towers) may be mentioned as appropriate for context or where drinking water information is specifically lacking, but drinking water focus will be critical.

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Lit Review Strategy• See outline (attached to email):

o Follows the four Research Tracks and is generally organized by each individual pathogen.

o Participants in each Track will be assigned a pathogen or other specific research question and use the corresponding outline to guide the lit review.

o Conference calls will aid in steering the lit review and also in cross-dissemination.

o After the outline filled in, Virginia Tech team will work to integrate the material, improve consistency, and identify emergent themes.

o VT students available to assist as needed

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Wiki Site, Google Docs, Zotero

• Wiki Site: Central forum for information and communication about Project 4379.

• Google Documents: Emerging literature review for each Track will be maintained as a Google Document. Enter draft lit review sections as they are ready for all to see and refer to.

• Zotero: Management of citations will be critical. Upload citations to Zotero with comments to classify where the information goes in the literature review. There will be one Zotero database for all four tracks

• (Randi Lieberman- overview)

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Schedule & Deadlines• Your immediate response needed to four emails:

o Invite to Wiki siteo Invite to Google documentso Invite to Zoteroo Email from your Track leader giving you your charge, requesting your ranked

topic selection, and providing link to doodle poll to schedule the three Track conference calls.

• Input on Literature Review Outline (optional)o By September 13th, enter your comments into the Lit Review Outline google

doc.• By Sept. 13th, Respond to your Track Leader’s email

with your ranked order of preference for the section you will write, as well as your response to doodle poll to schedule conference callso Track leader will confirm your topic asap

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Schedule & Deadlines: Conference Calls (~45 min. ea.)

• First Track conference call:o Week of Sept. 26o Prior to the conference call, upload your outline to google docs and

key references to Zoteroo Participants provide overview of main points they plan to covero Offer guidance to other Track members, e.g., missing references for their

question• Second Track conference call:

o Week of Oct. 31/Nov. 1sto One week prior, Upload draft final section to google docs and

corresponding references to Zotero.o Track members will have read each others’ sections prior to the call and

will offer input, coordinate sections, etc.• Third Track conference call:

o Week of Dec. 5th

o One week prior, Upload final section and references.o Final discussion of Track section and strategy for integrating with the

Literature Review document as a whole.

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Schedule & Deadlines: Leading Up to the

Workshop• Input on Whole Literature Review (Optional):

o Jan. 13th, 2012- Request any comments on document as a whole. Participants may access the google document and enter their comments using the Wiki site.

• Document Synthesis & Integration:o Jan. –Feb., 2012o Virginia Tech working internally to synthesize and integrate document,

make appropriate Figures, Tables, etc.• Draft Final Literature Review Posted for Review:

o Week of March 5th, 2012o Participants must review prior to the Workshop

• Workshop!o Monday March 26-Tuesday March 27, 2012

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Questions?

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