water treatment technology (tas 3010) lecture notes 2 - environmental microbiology

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Izan Jaafar, Engineering Science, FST, UMT Izan Jaafar, Engineering Science, FST, UMT © SHAHRUL ISMAIL, DESc. University College of Science and Technology Malaysia CHAPTER 3: Environmental Microbiology CHAPTER 2 : CHAPTER 2 : TAS 3101 : WATER TREATMENT TECHNOLOGY Environmental Environmental Microbiology Microbiology

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WATER TREATMENT TECHNOLOGY (TAS 3010) LECTURE NOTESUNIVERSITY MALAYSIA TERENGGANU 2009Disclaimer: I don't own this file. If you believe you do, and you don't want it to be published here, please let me know. I will remove it immediately

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Page 1: WATER TREATMENT TECHNOLOGY (TAS 3010) LECTURE NOTES 2 - Environmental Microbiology

Izan Jaafar, Engineering Science, FST, UMTIzan Jaafar, Engineering Science, FST, UMT

© SHAHRUL ISMAIL, DESc.University College of Science and Technology Malaysia

CHAPTER 3:Environmental Microbiology

CHAPTER 2 : CHAPTER 2 : CHAPTER 2 : CHAPTER 2 :

TAS 3101 : WATER TREATMENT TECHNOLOGY

Environmental Environmental

Microbiology Microbiology

Environmental Environmental

Microbiology Microbiology

Page 2: WATER TREATMENT TECHNOLOGY (TAS 3010) LECTURE NOTES 2 - Environmental Microbiology

Izan Jaafar, Engineering Science, FST, UMTIzan Jaafar, Engineering Science, FST, UMT

Menu

1) Definition

2) Microbial Interests

3) Microbiological Contaminants

4) Microorganism in environmental

engineering

5) Bacterial Growth and Phases

Page 3: WATER TREATMENT TECHNOLOGY (TAS 3010) LECTURE NOTES 2 - Environmental Microbiology

Izan Jaafar, Engineering Science, FST, UMTIzan Jaafar, Engineering Science, FST, UMT

Introduction

““The study of The study of microbes associated with

Water, drinking water, and Water, drinking water, and

wastewater (and other organic and wastewater (and other organic and

inorganic wastes) as well as inorganic wastes) as well as

Pollutant Degradation” Pollutant Degradation”

Environmental Microbiology Environmental Microbiology

Page 4: WATER TREATMENT TECHNOLOGY (TAS 3010) LECTURE NOTES 2 - Environmental Microbiology

Izan Jaafar, Engineering Science, FST, UMTIzan Jaafar, Engineering Science, FST, UMT

Introduction

““Tiny creatures that individually are single cell Tiny creatures that individually are single cell

organism, too small to be seen with the organism, too small to be seen with the

unaided eyeunaided eye””

What is microbes?? What is microbes??

Page 5: WATER TREATMENT TECHNOLOGY (TAS 3010) LECTURE NOTES 2 - Environmental Microbiology

Izan Jaafar, Engineering Science, FST, UMTIzan Jaafar, Engineering Science, FST, UMT

The Interfaces…..

Page 6: WATER TREATMENT TECHNOLOGY (TAS 3010) LECTURE NOTES 2 - Environmental Microbiology

Izan Jaafar, Engineering Science, FST, UMTIzan Jaafar, Engineering Science, FST, UMT

Microbial Interests

• Microbial roles of interest:

– disease production

– degradation of materials

– production of harmful

substances and/or

conditions

Page 7: WATER TREATMENT TECHNOLOGY (TAS 3010) LECTURE NOTES 2 - Environmental Microbiology

Izan Jaafar, Engineering Science, FST, UMTIzan Jaafar, Engineering Science, FST, UMT

Pathogens in Drinking Water

Page 8: WATER TREATMENT TECHNOLOGY (TAS 3010) LECTURE NOTES 2 - Environmental Microbiology

Izan Jaafar, Engineering Science, FST, UMTIzan Jaafar, Engineering Science, FST, UMT

Pathogens in Surface Water

Page 9: WATER TREATMENT TECHNOLOGY (TAS 3010) LECTURE NOTES 2 - Environmental Microbiology

Izan Jaafar, Engineering Science, FST, UMTIzan Jaafar, Engineering Science, FST, UMT

Pathogens in Surface Water

Page 10: WATER TREATMENT TECHNOLOGY (TAS 3010) LECTURE NOTES 2 - Environmental Microbiology

Izan Jaafar, Engineering Science, FST, UMTIzan Jaafar, Engineering Science, FST, UMT

Pathogens in Surface Water

Page 11: WATER TREATMENT TECHNOLOGY (TAS 3010) LECTURE NOTES 2 - Environmental Microbiology

Izan Jaafar, Engineering Science, FST, UMTIzan Jaafar, Engineering Science, FST, UMT

Introduction

- Microorganisms that play animportant rule in environmentalengineering :

• Bacteria• Viruses• Fungi • Algae• Protozoa

- The important of theseorganisms:

- Causes diseases towards humans- Decomposition of organic and inorganic

Page 12: WATER TREATMENT TECHNOLOGY (TAS 3010) LECTURE NOTES 2 - Environmental Microbiology

Izan Jaafar, Engineering Science, FST, UMTIzan Jaafar, Engineering Science, FST, UMT

• Small organisms - size between 0.5 – 5.0 m

• Present individually - pairs or in chain

• Different kind of shape : rod (bacillus), spiral and sphere

• Double themselves within 15 – 30 minute in suitable condition

Bacteria

Page 13: WATER TREATMENT TECHNOLOGY (TAS 3010) LECTURE NOTES 2 - Environmental Microbiology

Izan Jaafar, Engineering Science, FST, UMTIzan Jaafar, Engineering Science, FST, UMT

Classification of bacteria

According to the utilization of dissolved

oxygen :

• Aerobic : Use DO during decomposition of organic compound

• Anaerobic : Using O2 in the form of different compound

• Facultative: Able to survive in both of the above condition

Page 14: WATER TREATMENT TECHNOLOGY (TAS 3010) LECTURE NOTES 2 - Environmental Microbiology

Izan Jaafar, Engineering Science, FST, UMTIzan Jaafar, Engineering Science, FST, UMT

Disease caused by bacteria

• Typhoid fever : Caused??

• Cholera : Caused ??

• Jaundice fever : Caused??

Salmonelly typhi

Vibrio cholerae

Leptospira

Page 15: WATER TREATMENT TECHNOLOGY (TAS 3010) LECTURE NOTES 2 - Environmental Microbiology

Izan Jaafar, Engineering Science, FST, UMTIzan Jaafar, Engineering Science, FST, UMT

Page 16: WATER TREATMENT TECHNOLOGY (TAS 3010) LECTURE NOTES 2 - Environmental Microbiology

Izan Jaafar, Engineering Science, FST, UMTIzan Jaafar, Engineering Science, FST, UMT

Viruses

• Smallest microorganism – size range from 0.01 – 0.3 m

• Required host for survival• Cause diseases such as

hepatitis, flu, jaundice, polio and aids

Page 17: WATER TREATMENT TECHNOLOGY (TAS 3010) LECTURE NOTES 2 - Environmental Microbiology

Izan Jaafar, Engineering Science, FST, UMTIzan Jaafar, Engineering Science, FST, UMT

Fungi • Plants that unable to do

photosynthesis such as moulds and yeast

• Yeast – used for fermentation – making bread, cake and alcohol

• Moulds – filament– live in acidic condition – reduce the efficiency of

secondary sedimentation tank

– cause unpleasant smell and taste

Page 18: WATER TREATMENT TECHNOLOGY (TAS 3010) LECTURE NOTES 2 - Environmental Microbiology

Izan Jaafar, Engineering Science, FST, UMTIzan Jaafar, Engineering Science, FST, UMT

Page 19: WATER TREATMENT TECHNOLOGY (TAS 3010) LECTURE NOTES 2 - Environmental Microbiology

Izan Jaafar, Engineering Science, FST, UMTIzan Jaafar, Engineering Science, FST, UMT

Algae

• Plant that generate energy by photosynthesis

• Increase the DO level in the water. When??

• What if too much algae??

Present of sun light-Ph

- Taste and smel l- Light penetration- Die off – disintegrate and caused anaerobic condi tion

Page 20: WATER TREATMENT TECHNOLOGY (TAS 3010) LECTURE NOTES 2 - Environmental Microbiology

Izan Jaafar, Engineering Science, FST, UMTIzan Jaafar, Engineering Science, FST, UMT

Page 21: WATER TREATMENT TECHNOLOGY (TAS 3010) LECTURE NOTES 2 - Environmental Microbiology

Izan Jaafar, Engineering Science, FST, UMTIzan Jaafar, Engineering Science, FST, UMT

Protozoa

• Unicell organism – size 10 – 100 m• Use bacteria and algae as source of food

Page 22: WATER TREATMENT TECHNOLOGY (TAS 3010) LECTURE NOTES 2 - Environmental Microbiology

Izan Jaafar, Engineering Science, FST, UMTIzan Jaafar, Engineering Science, FST, UMT

• Could cause disease related to stomach and gastro intestine– e.g.: cryptosporidium : cryptosporidiosis (diarrhea)

• giardis lamblia : giardiasis (diarrhea)

Protozoa

Page 23: WATER TREATMENT TECHNOLOGY (TAS 3010) LECTURE NOTES 2 - Environmental Microbiology

Izan Jaafar, Engineering Science, FST, UMTIzan Jaafar, Engineering Science, FST, UMT

Page 24: WATER TREATMENT TECHNOLOGY (TAS 3010) LECTURE NOTES 2 - Environmental Microbiology

Izan Jaafar, Engineering Science, FST, UMTIzan Jaafar, Engineering Science, FST, UMT

Bacterial Growth

• Generally they reproduce binary fission (i.e. by dividing, the original cell becomes two new organisms)

Page 25: WATER TREATMENT TECHNOLOGY (TAS 3010) LECTURE NOTES 2 - Environmental Microbiology

Izan Jaafar, Engineering Science, FST, UMTIzan Jaafar, Engineering Science, FST, UMT

Phases of Bacteria

Page 26: WATER TREATMENT TECHNOLOGY (TAS 3010) LECTURE NOTES 2 - Environmental Microbiology

Izan Jaafar, Engineering Science, FST, UMTIzan Jaafar, Engineering Science, FST, UMT

Lag Phase :

• The time required for the organism to acclimate to their new environment and begins to divide.

Page 27: WATER TREATMENT TECHNOLOGY (TAS 3010) LECTURE NOTES 2 - Environmental Microbiology

Izan Jaafar, Engineering Science, FST, UMTIzan Jaafar, Engineering Science, FST, UMT

Log / exponential growth phase :

• The presence of access substrate promotes the maximum rate of growth possible, limited only by the ability of the bacteria to reproduce.

Page 28: WATER TREATMENT TECHNOLOGY (TAS 3010) LECTURE NOTES 2 - Environmental Microbiology

Izan Jaafar, Engineering Science, FST, UMTIzan Jaafar, Engineering Science, FST, UMT

Stationary Phase :

• Population remains stationary. Reasons for this phenomenon: – Cell have exhausted the substrate all nutrients necessary for

growth. – Growth of the new cells is offset by the death of whole cells.

Page 29: WATER TREATMENT TECHNOLOGY (TAS 3010) LECTURE NOTES 2 - Environmental Microbiology

Izan Jaafar, Engineering Science, FST, UMTIzan Jaafar, Engineering Science, FST, UMT

Death endogenous phase:

• Depletion of nutrient/food and toxic by-products of cell metabolism inhibit further growth.

• Bacteria death rate exceeds the production of new cell.

Page 30: WATER TREATMENT TECHNOLOGY (TAS 3010) LECTURE NOTES 2 - Environmental Microbiology

Izan Jaafar, Engineering Science, FST, UMTIzan Jaafar, Engineering Science, FST, UMT

“Acquire nutrients, produce energy, biosynthesize, grow,

reproduce, respond to environment, interact,

develop, differentiate, evolve, die”

Microbial Perspectives