wako product update

No.16

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ANALYTICAL CHEMISTRY

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Please visit the Wako Online Catalog

http://www.e-reagent.com

1. Food Analysis

A. Fructooligosaccharide Analysis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2

B. Azo Dye Analysis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2

C. Aspartame Analysis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3

D. Mycotoxin Analysis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3

E. Agricultural Chemical Residues in Foods Listed in the Japanese

Positive List System

• Simultaneous Analysis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4

F. Standards for Pesticide Residue Analysis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

G. Standards for Veterinary Drugs Listed in the Japanese Positive List System . . . 11

2. High Purity Solvents and Reagents

A. LC/MS Solvents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

B. HPLC Solvents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

3. Chromatography Products

A. Wakopak® Fluofi x®-II 120E . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

B. Syringe-type Solid-Phase Extraction Column . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

4. Environmental Analysis

A. Solvents for PCB Analysis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

B. Solvents for Dioxins Analysis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

5. Standards for Crude Drug Test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

Alphabetical Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

Online Catalog : http://www.e-reagent.com

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Wako Product Update No.16

A. Fructooligosaccharide AnalysisTh is set consists of 500mg vial each of 1-Kestose, Nystose and 1F-Fructofuranosylnystose.

It is applicable to analyze the fructooligosaccharide components contained in foods and beverages.

Simultaneous analysis of the Mixture of three fructooligosaccharides

Wako Cat. No. (Pkg. Size) Description Specifi cation

298-64101 (1 set)

Fructooligosaccharides Setfor HPLC Analysis

Kit Contents:

1 1-Kestose, 99.0+% (HPLC) .......................................................1 vial x 500 mg

2 Nystose, 99.0+% (HPLC) ............................................................1 vial x 500 mg

3 1F-Fructofranosylnystose, 80.0+% (HPLC) ..............1 vial x 500 mg

1 1-Kestose Specifi c rotation[α]D

20(c=5, H2O): +28.5 ~ +29.5˚ Water ≦ 5.0%

2 Nystose Specifi c rotation[α]D

20(c=5, H2O): +10.0 ~ +11.5˚ Water ≦ 5.0%

3 1F-Fructofranosylnystose Specifi c rotation[α]D

20(c=5, H2O): -2.5 ~ -1.5˚ Water ≦ 5.0%

RelatedProducts

Wako Cat. No. (Pkg. Size) Description Specifi cation

116-00431 (250 mg)

112-00433 (1 g)

1-Kestose, 99.0+% (HPLC)

CAS No.: 470-69-9

Solubility: Soluble in water. Practically insoluble in ethanol and acetone.

Solubility in water: to pass testSpecifi c rotation [α]D

20 (c=5, H2O): +28 ~ +32˚Water≦ 0.5%TLC test : to pass test

147-05981 (250 mg)

143-05983 (1 g)

Nystose Trihydrate

CAS No.: 139523-49-2

Solubility: Soluble in water and ethanol. Practically insoluble in acetone.

Solubility in water: to pass testSpecifi c rotation [α]D

20 (c=5, H2O): +9.2 ~ +10.2˚Water≦ 0.5%TLC test : to pass test

B. Azo Dye AnalysisSudan I, II, and Para Red, oil-soluble synthetic dyes (azo dyes), are unauthorized food additives in Japan, Europe, and the United States.

Wako Cat. No. (Pkg. Size) Description Grade

193-14131 (200 mg)

Sudan I Standard, 98.0+% (HPLC)Chemical Name : 1-Phenylazo-2-naphtol

CAS No.: 842-07-9

Solubility: Practically insoluble in water. Readily soluble in ethanol,

acetone, benzene.

N N

HO

C16H12N2O=248.28

for

HPLC

Analysis

190-14141 (200 mg)

Sudan II Standard, 98.0+% (HPLC)Chemical Name : 1-(2,4-Dimethylphenylazo)-2-naphthol

CAS No.: 3118-97-6

Solubility: Practically insoluble in water. Readily soluble in ethanol,

acetone, chloroform.

N N

HO

CH3

H3C

C18H16N2O=276.33

160-22171 (200 mg)Para Red Standard, 98.0+% (HPLC)

Chemical Name: 1-(4-Nitrophenylazo)-2-naphthol

CAS No.: 6410-10-2

N N

HO

O2N

C18H11N3O3=293.28

CH2OH

HOH2C

OH

OH

HO

HO

HO

O

OCH2

H

n

O

n=2 1-Kestose (Glu-Fru2)n=3 Nystose (Glu-Fru3)n=4 1F-Fructofuranosylnystose (Glu-Fru4)

[HPLC conditions]

Column: Wakopak® WS 5NH2, 4.6 x 250 mm Eluent: CH3CN/H2O = 70/30 (v/v) Flow rate: 1.5 mL/min. at 40˚C Detection: RI Injection: 10 mg each/mL, 10 µL

1 1-Kestose

2 Nystose

3 1F-Fructofranosylnystose

4 a structual isomer of

1F-Fructofranosylnystose

1. Food Analysis

Analytical Chemistry

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C. Aspartame AnalysisLD-Aspartame is used as the reference material for the analysis of "other enantiomer",

which is one of the test items of "Aspartame [LL-Aspartame] analysis" in Japanese Standard

of Food Additives. Since the LD-Aspartame does not have sweatness, the limit of

LD-aspartame is specifi ed.

Description Wako Cat. No. Pkg. Size Solubility

L-α-Aspartyl-D-Phenylalanine Methyl Ester, 95.0+% (HPLC) [LD-Aspartame]

010-20401 200 mgSoluble in water

016-20403 1 g

D. Mycotoxin AnalysisPatulin is a mycotoxin, produced by certain species of Penicillium and Aspergillus. Apple juice is well-

known to be the major source of contamination, and according to offi cial specifi cations, the advisory

limit of patulin in apple juice and the concentrates is less than 50ppb.

Th is product is for a standard for analyzing the patulin contained in apple juice and the concentrates.

Description Wako Cat. No. Pkg. Size Assay(HPLC) Grade CAS No. Chemical Formula (MW)

Patulin, from Penicillium expansum

168-21631 10 mg 98.5+% for Mycotoxin Assay 149-29-1 C7H6O4 (154.12)

RelatedProducts

Description Wako Cat. No. Pkg. Size Assay Grade CAS No. Chemical Formula (MW)

Butenolide 024-07761 10 mg 98.0+% (HPLC) for Mycotoxin Assay 16275-44-8 C6H7NO3 (141.13)

Citrinin Standard 033-08011 10 mg 1 spot (TLC) - 518-75-2 C13H14O5 (250.25)

Deoxynivalenol Standard 048-18631 10 mg 98.0+% (HPLC) for Mycotoxin Assay 51481-10-8 C15H20O6 (296.13)

Diacetoxyscirpenol 046-20401 1 mg 98.0+% (HPLC) for Mycotoxin Assay 2270-40-8 C19H26O7 (366.41)

Fumonisin B1 060-03563 10 mg 90.0+% (HPLC) for Biochemistry 116355-83-0 C34H59NO15 (721.84)

Neosolaniol Standard 141-04661 10 mg 97.0+% (GC) for Mycotoxin Assay 36419-25-2 C19H26O8 (382.41)

Nivalenol Standard 144-04651 10 mg 97.0+% (GC) for Mycotoxin Assay 23282-20-4 C15H20O7 (312.32)

Ochratoxin A 150-02111 5 mg 97.0+% (HPLC) for Biochemistry 303-47-9 C20H18ClNO6 (403-81)

T-2 Toxin Standard 209-08251 10 mg 97.0+% (GC) for Mycotoxin Assay 21259-20-1 C24H34O9 (466.53)

1. Food Analysis


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C14H18N2O5=294.30L-A-Aspartyl-D-Phenylalanine Methyl Ester


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E. Agricultural Chemical Residues in Foods Listed in the Japanese Positive List SystemIn accordance with the Law to Partially Revise the Food Sanitation Law, etc. (Law No. 55, 2003), standards regarding residual pesticides in

foods, veterinary drugs, and food additives have been established. As a result, the Japanese positive list system that regulates the circulation of

foods that contain pesticides whose levels exceed the advisory limits has become eff ective as of May 29, 2006.

We strive to commercialize standard products that meet the requirements of this positive list system.

Simultaneous Analysis In anticipation of the enforcement of the positive list system, a notifi cation of simultaneous test was transmitted by the Ministry of Health,

Labor, and Welfare in November 2005 (Syoku-an No. 1129002). Each is a pesticide mixture that can be used for the simultaneous test.

* "No." refers to the number designated to the each of the components listed in the "Provisional Maximum Residue Limits for Agricultural Chemicals in Foods (fi nal draft)".

**: Component not listed in the fi nal draft but the residual limit is regulated.

Description Wako Cat. # Pkg. Size Components

Pesticide Mixture PL-1-1 (20μg/mL each in acetone) 169-22261 5 Ampules x 1 mL 32 pesticides

Components No.(*) Components No. Components No.

azinphosmethyl 32 spiroxamine 300 fenpropimorph 524

atrazine 39 thiobencarb 355 fl ucythrinate 554

beta-endosulfan 112 tefl uthrin 375 fl uvalinate 561

oxadiazon 118 terbutryn 381 procymidone 576

omethoate 132 terbufos 382 trans-permethrin 619

kresoxim-methyl 166 trifl uralin 403 cis-permethrin 619

chlorpyriphos-methyl 194 norfl urazon 440 penconazol 620

chlorfenapyr 195 bifenthrin 473 pendimethalin 631

cyfl uthrin 272 pyrethrins 497 malathion 652

difl ufenican 274 fenamiphos 504 methidathion 674

dimethoate 290 fenarimol 505




EPN 03** mepronil 59** tribuphos 399

isoprocarb 04** cadusafos 1** pyrafl ufen-ethyl 484

esprocarb 08** acrinathrin 22 pyrimethanil 495

diclocymet 21** acetochlor 36 fenothiocarb 510

(Z)-Dimethylvinphos 25** isoxathion 66 fl uthiacet-methyl 557

simetryn 26** iprobenfos 75 fl umiclorac pentyl 568

thenylchlor 31** imibenconazole debenzyl form 87 propachlor 581

tricyclazole 34** etofenprox 100 prometryn 598

bifenox 40** chlorpropham 200 bromophos 603

(E)-Pyrifenox 43** dimethametryn 286 benalaxyl 614

fensulfothion 48** zoxamide 341 phosalone 635

prothiofos 54** terbacil 342

benfuresate 57** tetraconazole 368




alpha-BHC 01 Quinoclamine 157 phenothrin 511

edifenphos 11 Cyanazine 221 Butamifos 530

Cafenstrole 16 Cyanophos 223 fl amprop-methyl 541

Cyhalofop-butyl 24 Dichlofenthion 238 Flumioxazin 567

thifl uzamide 29 Diclofop methyl 243 Propazine 582

tolfenpyrad 36 Diphenamid 265 Bromacil 595

(Z)-Pyrifenox 43 Tebufenpyrad 373 Bromobutide 601

pyributicarb 44 Trifl oxystrobin 404 Hexaconazole 608

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1. Food Analysis


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Fenoxanil 47 Napropamide 421 benoxacol 615

prohydrojasmon 55 nitrothal-isopropyl 432 Benfl uralin 633

XMC 20 Paclobutrazo 446 mefenoxam 672

oxadixyl 119 Piperophos 476

quinalphos 155 Pyrazophos 482




Azamethiphos 27 Chromafenozide 183 Pyrazolynate 483

Azinphos-Methyl 32 Chloridazon 186 Pyriftalid 490

Anilofos 41 Cyazofamid 220 Fenoxycarb 508

Abamectin�B1a 42 Cyfl ufenamid 273 Ferimzone (E) 513

Isoxafl utole 67 Simeconazole 285 Ferimzone (Z) 513

Iprovalicarb 74 Dimethirimol 288 Butafenacil 529

Indoxacarb 88 Thiacloprid 347 Furathiocarb 539

Oryzalin 134 Thiabendazole 350 Benzofenap 628

Cloquintocet-Mexyl 170 Thiamethoxam 352 Milbemectin A3 657

Clothianidin 176 Naproanilide 420 Methoxyfenozide 679




1-Naphthaleneacetic Acid 3 Diclosulam 237 Flumetsulam 570

4-CPA 11 Dichlorprop 245 Fluroxypyr 573

MCPB 16 Gibberellin 281 Bromoxynil 599

Ioxynil 21 Thidiazuron 357 Florasuram 605

Acifl uorfen 28 Thifensulfuron-Methyl 359 Fomesafen 642

Cloprop 181 Triclopyr 393 Forchlorfenuron 646

Cloransulam-Methyl 185 Haloxyfop 456 Mecoprop 661




EPTC 14 Chloroneb 208 Fenoxaprop-ethyl 507

Iprodione 73 Diphenylamine 267 Phenobucarb 512

Iprodione metabolite 73 Bioresmethrin 462 Esfenvalerate 519

Imazalil 82 Piperonyl Butoxide 475 Flusilazole 555

Etridiazole 103 Pirimicarb 492 Prochloraz 575

Endosulfan sulfate 112 Famphur 501 Propetamphos 591




Acephate 37 aldoxycarb 55 Bendiocarb 629

Azoxystrobin 38 Carbaryl 144 Metalaxyl 672

Aldicarb 54 Thiabendazole 350 Metribuzin 585

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1. Food Analysis



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Wako Cat. No.(Package Size)

Description Chemical Structure/Chemical Formula/CAS No.

016-20361

(200mg)

Abamectin Standard, 95.0+% (HPLC)

<insecticide>Mixture of Avermectin B1a and B1b

Chemical Name:

B1a: (10E, 14E, 16E, 22Z)-(1R, 4S, 5'S, 6S, 6'R, 8R, 12S, 13S, 20R, 21R, 24S)-6'-[(S)-sec-Butyl]-21,

24-dihydroxy-5', 11, 13, 22-tetramethyl-2-oxo-3, 7, 19-trioxatetracyclo[15.6.1.14, 8.020,

24]pentacosa-10, 14, 16, 22-tetraene-6-spiro-2'-(5', 6'-dihydro-2'H-pyran)-12-yl 2, 6-Dideoxy-4-O-(2,

6-dideoxy-3-O-methyl-α-L-arabino-hexopyranosyl)-3-O-methyl-α-L-arabino-hexopyranoside

B1b: (10E, 14E, 16E, 22Z)-(1R, 4S, 5'S, 6S, 6'R, 8R, 12S, 13S, 20R, 21R, 24S)-21, 24-dihydroxy-6'-

isopropyl-5', 11, 13, 22-tetramethyl-2-oxo-3, 7, 19-trioxatetracyclo[15.6.1.14, 8.020, 24]pentacosa-10,

14, 16, 22-tetraene-6-spiro-2'-(5', 6'-dihydro-2'H-pyran)-12-yl 2, 6-Dideoxy-4-O-(2, 6-dideoxy-3-O-

methyl-α-L-arabino-hexopyranosyl)-3-O-methyl-α-L-arabino-hexopyranoside

Another Name: Agrimec

Solubility: In water 7-10μg/L (20°C). In toluene 350, acetone 100, isopropanol 70, chloroorm 25,

ethanol 20, methanol 19.5, n-butanol 10, cyclohexane 6 (all in g/L, 21°C)

O

HO

O

OCH3

H3C

O O

OCH3

H3C

CH3

O

H3C

O

CH3

H

CH3

R

OO

OCH3

H

OH

H

H

OH

H

R=-CH2CH3 (Avermectin B1a)R=-CH3 (Avermectin B1b)

CAS No. 71751-41-2B1a:C48H72O14 = 873.08 B1b:C47H70O14 = 859.05

010-20521

(200mg)

Acifl uorfen Standard, 98.0+% (cGC)

<herbicide>Chemical Name: 5-(2-5-(2-Chloro-α,α,α-trifl uoro-p-tolyloxy)-2-nitrobenzoic Acid

Solubility: In water 120mg/L (23-25°C). In acetone 600, ethanol 500,

dichloromethan 50, xylene, kerosene <10 (all in g/kg, 25°C)

F3C O

Cl COOH

NO2

015-20451

(200mg)

4-Aminopyridine Standard, 99.0+% (cGC)<repellent>

Another Name: Avitrol-200

Solubility: Freely soluble in water, ethanol and acetone.

N

NH2

016-20241

(200mg)

Anilofos Standard, 98.0+% (HPLC)<herbicide>

Chemical Name: S-4-Chloro-N-isopropylcarbaniloylmethyl O,O-DimethylPhosphorodithioate

Another Name: Arozin

Solubility: In water 13.6mg/L (20°C)In acetone, chloroform,

toluene > 1,000, benzene, ethanol, dichloromethane,

ethyl acetate >200, hexane 12 (all in g/L)

022-15251

(200mg)

6-Benzylaminopurine Standared, 99.0+% (cGC)<plant growth regulator>

Chemical Name: 6-Benzyladenine

Another Name: BA

Solubility: Insoluble in general organic solvents.

Soluble in dimethylformamide and dimethyl sulfoxide.

020-15431

(200mg)

Bromochloromethane Standard, 98.0+% (cGC)

<fumigation> CH2Br Cl

024-15571

(100mg)

Butamifos Oxon Standard, 98.0+% (cGC)

Chemical Name: O-Ethyl O-6-Nitro-m-tolyl sec-ButylphosphoramidateNO2

H3C

O P

O

O

CH2CH3

N

C CH2CH3

CH3

H H

034-19771

(200mg)

Carboxin Standard, 98.0+% (cGC)

<bactericide>Chemical Name: 5,6-Dihydro-2-methyl-1,4-oxathiine-3-carboxanilide

Solubility: In water 199mg/L (25°C). In acetone 177, methylene chloride 353,

methanol 88, ethyl acetate 93 mg/L (25°C). S

O CH3

CONH

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1. Food Analysis


F. Standards for Pesticide Residue Analysis

CAS No. 64249-01-0C13H19ClNO3PS2 = 367.85

CAS No. 50594-66-6C14H7ClF3NO5 = 361.66

CAS No. 504-24-5C5H6N2 = 94.11

CAS No. 1214-39-7C12H11N5 = 225.25

CAS No. 74-97-5BrCH2Cl = 129.38

CAS No. 56362-05-1C13H21N2O5P = 316.29

CAS No. 5234-68-4C12H13NO2S= 235.30

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030-19751

(200mg)

Chloroxuron Standard, 98.0+% (HPLC)

<herbicide>Chemical Name: 3-[4-(4-Chlorophenoxy)phenyl]-1,1-dimethylurea

Solubility: Freely soluble in ethanol and acetone.

Practically insoluble in water.

Cl O NHCN(CH3)2

O

CAS No. 1982-47-4C15H15ClN2O2 = 290.74

030-19511

(200mg)

4-CPA Standard, 99.0+% (HPLC)<plant growth regulator>

Chemical Name: p-Chlorophenoxyacetic Acid

Another Name: Tomatotone

Solubility: Freely soluble in ethanol. Slightly soluble in water.

Cl OCH2CO2H

CAS No. 122-88-3C8H7ClO3 = 186.59

032-19711

(200mg)

Cyanamide Standard, 95.0+% (Ti)Solubility: Freely soluble in water, ethanol and acetone. NCH2N

CAS No. 420-04-2CH2N2 = 42.04

045-29371

(200mg)

2,6-Difl uorobenzoic Acid Standard, 98.0+% (Ti)<miticide>

Solubility: Soluble in ethanol and acetone. Slightly soluble in water.

044-29601

(200mg)

1,1-Dichloro-2,2-bis(4-ethylphenyl)ethane Standard, 98.0+% (cGC)<Insecticide>

Another Name: EthylanC2H5 CH C2H5

CHCl2

049-29411

(200mg)

Dichloroprop Standard, 99.0+% (HPLC)<plant growth regulator, herbicide>

Chemical Name: (RS)-2-(2,4-Dichlorophenoxy)propionic Acid

Another Name: Basagran

Solubility: In water 350 mg/L (20°C). In acetone 595, Isopropanol 510,

benzene 85, toluene 69, xylene 51, Kerosene 2.1 (g/L, 20°C)

043-29811

(200mg)

2,6-Diisopropylnaphthalene Standard, 98.0+% (cGC)

<plant growth regulator> HC

HCH3C

CH3

CH3

CH3

042-29401

(200mg)

DNOC Standard, 98.0+% (cGC)<insecticide>

Chemical Name: 4,6-Dinitro-o-cresol

Common Name: Ibertox

Solubility: In water 6.94 g/L (20°C, pH 7). In toluene 251, methanol 58.4,

hexane 4.03, Ethyl Acetate 338, Acetone 514, dichloromethane 503 (all in g/L, 20°C)

064-04661

(200mg)

Fenbendazole Standard, 98.0+% (HPLC)<vermicide>

Chemical Name: Methyl [5-(Phenylthio)-1H-benzimidazol-2-yl]carbamate

Solubility: Soluble in N,N-dimethylformamide. S N

HN

NH

OCH3

O

068-04681

(100mg)

Flufenpyl-ethyl Standard, 98.0+% (HPLC)<herbicide>

Chemical Name: Ethyl 2-Chloro-5-[1,6-dihydro-5-methyl-6-oxo- 4-(trifl uoromethyl) pyridazin-1-yl]-4-

fl uorophenoxyacetate

Solubility: Soluble in acetone. Practically insoluble in water and ethanol.

N

N

OH3C

F3C

F

Cl

OCH2COOC2H5

CAS No. 188489-07-8C16H13ClF4N2O4 = 408.73

7


CAS No. 385-00-2C7H4F2O2 = 158.10

CAS No. 72-56-0C18H20Cl2 = 307.26

CAS No. 120-36-5C9H8Cl2O3 = 235.06

CAS No. 24157-81-1C16H20 = 212.33

CAS No. 43210-67-9C15H13N3O2S= 299.35

CAS No. 534-52-1C7H6N2O5 = 198.13

1. Food Analysis



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062-04601

(200mg)

Fluridone Standard, 98.0+% (HPLC)<herbicide>

Chemical Name: 1-Methyl-3-phenyl-5-[3-(trifl uoromethyl)phenyl]-4(1H)-pyridinone

Another Name: Sonar

Solubility: In water 12 mg/L (25°C, pH 7). In methanol, chloroform, diethyl ether > 10, ethyl acetate >

5, hexane <0.5 in hexane (all in g/L)

066-04621

(200mg)

Forchlorfenuron Standard, 99.0+% (HPLC)<plant growth regulator>

Chemical Name: 1-(2-Chloro-4-pyridyl)-3-phenylurea

Common Name: Fulmet

Solubility: In water 39 mg/L (pH 6.4, 21°C).

N

Cl

NHCONH

078-05081

(200mg)

Gibberelline A3 Standard, 95.0+% (HPLC)<plant growth regulator>

Chemical Name: (3S,3aS,4S,4aS,6S,8aR,8bR,11S)-6,11-dihydroxy-3-methyl-12-methylene-2-oxo-4a,6-

ethano-3,8b-prop-1-eno-perhydroindeno[1,2-b]furan-4-carboxylic Acid

Another Name: Gibberellin Acid

Solubility: In water 5 g/L (room temperature), Soluble in methanol, ethanol, acetone, alkaline

solution. Slightly soluble in ethyl acetate. Insoluble in chloroform.CAS No. 77-06-5C19H22O6 = 346.37

081-08311

(200mg)

Hexazinone Standard, 98.0+% (cGC)

<herbicide>Chemical Name: 3-Cyclohexyl-6-(dimethylamino)-1-methyl-1,3,5-triazine-2,4-(1H,3H)-dione

Solubility: In water 33g/kg (25°C). In chloroform 3880, methanol 2650, benzene 940,

dimethylformamide 836, acetone 792, toluene 386, hexane 3 (all in g/kg, 25°C)N N

N

CH3

O(H3C)2N

O

118-00533

(100mg)

Kresoxim-methyl Standard, 99.0+% (cGC)

<bactericide>Chemical Name: Methyl (E)-2-Methoxyimino-2-[2-(o-tolyloxymethyl)phenyl]acetate

Solubility: In water 2.0 x 10-4, acetone 171.0, methanol 11.8, dichlor 1,244.6(mg/mL)

CH3

O

N

O

OH3C

OCH3

130-14841

(200mg)

Mepiquat Chloride Standard, 98.0+% (Ti)<plant growth regulator>

Chemical Name: 1,1-Dimethylpiperidinium Chloride

Another Name: Frastar

Solubility: In water > 500 g/kg (20°C). In ethanol 162, chloroform 10.5, acetone, benzene, ethyl

acetate, cyclohexane < 1.0 (all in g/kg, 20°C) CAS No. 24307-26-4C7H16NCl = 149.66

139-14791

(200mg)

Methyl Isothiocyanate Standard, 99.0+% (cGC)<bactericide, insecticide, herbicide>

Chemical Name: Methyl Isothiocyanate

Another Name: Trapex CH3N CS

CAS No. 556-61-6C2H3NS = 73.12

133-14831

(200mg)

1-Methylpiperidine Standard, 99.0+% (cGC)<plant growth regulator, mepiquat chloride metabolite>

Solubility: Soluble in water, alcohol, ether.

137-14971

(200mg)

MPP Oxon Standard, 98.0+% (cGC)

Chemical Name: O,O-Dimethyl O-3-Methyl-4-(methylthio)phenyl Phosphate

Another Name: Fenthion Oxon

Solubility: Soluble in acetone.H3CS

H3C

O P

O

OCH3

OCH3

8


CAS No. 68157-60-8C12H10ClN3O = 247.68

CAS No. 51235-04-2C12H20N4O2 = 252.31

CAS No. 143390-89-0C18H19NO4 = 313.35

CAS No. 626-67-5C6H13N = 99.17

CAS No. 6552-12-1C10H15O4PS = 262.26

1. Food Analysis


CAS No. 59756-60-4C19H14F3NO = 329.32

Fo

od

An

aly

sis




136-15161

(100mg)

MPP Oxon Sulfoxide Standard, 98.0+% (cGC)

Chemical Name: O,O-Dimethyl O-3-Methyl-4-methylsulfi nylphenyl Phosphate

S

H3C

O

H3C O P(OCH3)2

O

148-08211

(100mg)

Naproanilide Standard, 99.0+% (HPLC)<herbicide>

Chemical Name: 2-(2-Naphthyloxy)-N-phenylpropionamide

Another Name: Uribest

Solubility: In water 0.75 mg/L (27°C). In acetone 171, toluene 42,

ethanol 17, benzene 46 (all in g/L, 27°C).

140-08271

(200mg)

Naptalam Standard, 98.0+% (HPLC)<herbicide>

Chemical Name: N-1-Naphthylphthalamic Acid

Another Name: Alanap

Solubility: In water 200 mg/L (20°C). In acetone 5, dimethylformamide 39,

dimethyl sulfoxide 43, methylethyl ketone 4, isopropanol 2, carbon tetrachloride 0.1 (g/kg).

COOH

CONH

141-08201

(200mg)

Nitrapyrin Standard, 98.0+% (GC)<bactericide>

Chemical Name: 2-Chloro-6-trichloromethylpyridine

Another Name: N-Serve

Solubility: In water 72 ppm (25°C). In ethanol 300 g/kg (20°C), acetone 1.98,

dichloromethane 1.85, xylene 1.04 (kg/kg, 26°C).

155-02541

(200mg)

Oxfendazole Standard, 98.0+% (HPLC)<anthelminthic>

Chemical Name: Methyl [5-(Phenylsulfi nyl)-1H-benzimidazol-2-yl]carbamate

Solubility: Soluble in N,N-dimethylformamide

S N

HN

NH

OCH3

O

O

163-21941

( 00mg)

Pyridalyl Standard, 98.0+% (HPLC)<insecticide>

Chemical Name: 2,6-Dichloro-4-(3,3-dichloroallyloxy)phenyl-3-[5-(trifl uoromethyl)-2-

pyridyloxy]propyl Ether

Other Name: Pyridalyl

Solubility: soluble in acetone. Slightly soluble in water.

N O O

Cl O

Cl

ClF3C

Cl

168-21751

(200mg)

Pyrimethanil Standard, 98.0+% (cGC)<bactericide>

Chemical Name: N-(4,6-Dimethylpyrimidine-2-yl) aniline

Solubility: In water 0.121g/L (pH 6.1, 25°C). In acetone 389,

ethyl acetate 617, methanol 176, methylene chloride 1000, n-hexane 23.7,

toluene 412 (all in g/L, 20°C)

N

N

N

H

CH3

CH3

193-13651

(100mg)

Sulprofos Oxon Standard, 98.0+% (cGC)<plant growth regulator>

Standard for sulprofos analysis

Chemical Name: O-Ethyl O-Methylthiophenyl-S-propylphosphorothioate

Solubility: Soluble in acetone.

9


CAS No. 6552-13-2C10H15O5PS = 278.26

CAS No. 52570-16-8C19H17NO2 = 291.34

CAS No. 132-66-1C18H13NO3 = 291.30

CAS No. 1929-82-4C6H3Cl4N = 230.91

CAS No. 53716-50-0C15H13N3O3S = 315.35

CAS No. 179101-81-6C18H14Cl4F3NO3 = 491.12

CAS No. 53112-28-0C12H13N3 = 199.25

CAS No. 38527-90-1C12H19O3PS2 = 306.38

1. Food Analysis



Fo

od

An

aly

sis




201-16261

( 00mg)

TCA-sodium Standard, 95.0+% (Ti) <herbicide>

Chemical Name: Sodium Trichloroacetate (Sodium TCA)

Another Name: Erbitox

Solubility: In water 1.2kg/L (25°C). In methanol 232, acetone 7.6, diethylether 0.2, benzene 0.07,

carbone tetrachloride 0.04, heptane 0.02 (g/L, 25°C).

Cl3CCO2Na

CAS No. 650-51-1C2Cl3NaO2 = 185.37

209-16441

(200mg)

Tecnazene Standard, 98.0+% (cGC)<bactericide>

Chemical Name: 1,2,4,5-Tetrachloro-3-nitrobenzene

Solubility: In water 0.44mg/L (20°C), In ethanol 40 g/L (25°C).

NO2

Cl

ClCl

Cl

203-16221

(200mg)

Thidiazuron Standard, 98.0+% (HPLC)<plant growth regulator>

Chemical Name: 1-Phenyl-3-(1,2,3-thiadiazol-5-yl)urea

Common Name: Dropp

Solubility: In water 31 mg/L (pH 7, 25°C), hexane 0.002, methanol 4.20, dichloromethane 0.003,

toluene 0.400, acetone 6.67, ethyl acetate 1.1 (g/L, 20°C) CAS No. 51707-55-2C9H8N4OS = 220.25

204-16251

(100mg)

Tiadinil Standard, 98.0+% (HPLC)<bactericide>

Chemical Name: 3’-Chloro-4,4’-dimethyl-1,2,3-thiadiazole-5-carboxanilide

Another Name: VGET

Solubility: In water 0.0132 g/L (pH 6.13~6.48, 20°C). Hexane 0.0740, toluene 11.8, dichloromethane

156. In acetone 434, methanol 124, ethyl acetate 198 (g/L, 20°C).

CAS No. 223580-51-6C11H10ClN3OS = 267.73

202-16551

(100mg)

Tolclofos-methyl Oxon Standard, 98.0+% (cGC)

Chemical Name: O-2,6-Dichloro-p-tolyl O,O-Dimethyl Phosphate

H3C O P

O

Cl

Cl

OCH3

OCH3

200-16231

(100mg)

m-Toluidine Standard, 99.0+% (cGC)

Another Name: Phenmedipham Metabolite

Solubility: Soluble in ethanol and acetone. Slightly soluble in water.

232-02281

( 00mg)

Warfarin Standard, 99.0+% (HPLC)<rodenticide>

Chemical Name: 3-(α-Acetonylbenzyl)-4-hydroxycoumarin

Another Name: Sakarat

Solubility: In water 17mg/L (20°C). In acetone 65, chloroform 56, dioxane 100 (g/L, 20°C). Slightly

soluble in benzene, ether, cyclohexane. Soluble in methanol, ethanol, isopropanol.

O

OH

O

CHCH2COCH3


CAS No. 97483-08-4C9H11Cl2O4P = 285.06

CAS No. 108-44-1C7H9N = 107.15

CAS No. 81-81-2C19H16O4 = 308.33

CAS No. 117-18-0C6HCl4NO2 = 260.89

01

1. Food Analysis


Fo

od

An

aly

sis


G. Standards for Veterinary Drugs Listed in the Japanese Positive List SystemVeterinary Drug Standard for HPLC Analysis

Amoxicillin Trihydrate Standard,

98.0+% (HPLC)

CAS No. 61336-70-7C16H19N3O5S・3H2O=419.45

Wako Cat. #018-20441 (200 mg)

Ampicillin Standard,

98.0+% (HPLC)

CAS No. 69-53-4C16H19N3O4S=349.40

Wako Cat. #017-20531 (200 mg)

Benzylpenicillin Potassium

Standard, 98.0+%(HPLC)

CAS No. 113-98-4C16H17KN2O4S=372.48

Wako Cat. #025-15501 (200 mg)

Betamethasone Standard Assay,

98.0+% (HPLC)

O

CH3

HOCH3

CH3

F H

OHO

OH

H

H

H

CAS No. 378-44-9C22H29FO5=392.46

Wako Cat. #026-15271 (200 mg)

Bithionol Standard, 98.0+% (HPLC)

CAS No. 97-18-7C12H6Cl4O2S=356.05

Wako Cat. #028-15351 (200 mg)

Butylhydroxsyanisole Standard

(mixture of isomers), 98.0+% (HPLC)

OCH3

OH

C(CH3)3

CAS No. 213-16-5C11H16O2=180.24

Wako Cat. #020-15311 (200 mg)

Chloramphenicol Standard,

98.0+% (HPLC)

HN

Cl

H OH

H O

Cl

OHO2N

CAS No. 56-75-7C11H12Cl2N2O5=323.13

Wako Cat. #037-19641 (200 mg)

Chlormadinone Acetate Standard,

99.0+% (HPLC)

O

Cl

H

CH3 H

CH3

H

CH3O

O

O

CH3

CAS No. 302-22-7C23H29CIO4=404.93

Wako Cat. #034-19531 (200 mg)

Chlorpromazine Hydrochloride

Standard, 98.0+% (HPLC)

S

N Cl

N

CH3

CH3

HCl

CAS No. 69-09-0C17H19ClN2S・HCl=355.33

Wako Cat. #036-19611 (200 mg)

Ciprofl oxacin Hydrochloride Monohydrate Standard, 98.0+% (HPLC)

CAS No. 86393-32-0C17H18FN3O3・HCl・H2O=385.82Wako Cat. #033-19621 (200 mg)

Dexamethasone Standard, 98.0+% (HPLC)

O

CH3

HOCH3

H

HF

OHO

OHCH3

CAS No. 50-02-2C22H29FO5=392.46

Wako Cat. #045-29491 (200 mg)

Dibutylhydroxytoluene Standard, 98.0+% (HPLC)

CH3

C(CH3)3

OH

(H3C)3C

CAS No. 128-37-0C15H24O=220.35

Wako Cat. #047-29451 (200 mg)

Dinitolmide Standard, 98.0+% (HPLC)

CAS No. 148-01-6C8H7N3O5=225.16

Wako Cat. #049-29531 (200 mg)

Eugenol Standard, 98.0+% (HPLC)

CAS No. 97-53-0C10H12O2=164.20

Wako Cat. #059-07351 (200 mg)

Hydrocortisone Standard,

98.0+% (HPLC)

O

CH3 H

HOCH3

C

O

CH2OH

OH

H

H

H

CAS No. 50-23-7C21H30O5=362.46

Wako Cat. #086-08241 (200 mg)

1 1

1. Food Analysis



Fo

od

An

aly

sis


Isocyanuric Acid Standard,

98.0+% (HPLC)

CAS No. 108-80-5C3H3N3O3=129.07

Wako Cat. #091-05311 (200 mg)

Meloxicam Standard,

98.0+% (HPLC)

CAS No. 71125-38-7C14H13N3O4S2=351.40

Wako Cat. #135-15131 (200 mg)

Metoclopramide Standard,

98.0+% (HPLC)

H2N

Cl

OCH3

CONHCH2CH2NC2H5

C2H5

CAS No. 364-62-5C14H22ClN3O2=299.80

Wako Cat. #132-14921 (200 mg)

Metronidazole Standard,

98.0+% (HPLC)

N

N CH3

CH2CH2OH

O2N

CAS No. 443-48-1C6H9N3O3=171.15

Wako Cat. #139-14931 (200 mg)

Nitroxynil Standard, 98.0+% (HPLC)

CAS No. 1689-89-0C7H3IN2O3=290.01

Wako Cat. #142-08231 (200 mg)

Norfl oxacin Standard, 98.0+% (HPLC)

HNN N

CH3

CO2H

O

F

CAS No. 70458-96-7C16H18FN3O3=319.33

Wako Cat. #149-08241 (200 mg)

Oxacillin Sodium Monohydrate

Standard, 98.0+% (HPLC)

NO CH3

CONH

N

S

CH3

CH3

H

HH

OCOONa

.H 2O

CAS No. 7240-38-2C19H18N3NaO5S ･ H2O=441.43

Wako Cat. #158-02531 (200 mg)

Oxyclozanide Standard,

98.0+% (HPLC)

NH

O

OH

Cl

Cl

OH

Cl

Cl

Cl

CAS No. 2277-92-1C13H6Cl5NO3=401.46

Wako Cat. #159-02561 (200 mg)

Potassium Clavulanate Standard, 98.0+% (HPLC)

CAS No. 61177-45-5C8H8KNO5=237.25

Wako Cat. #166-21791 (100 mg)

Prednisolone Standard, 98.0+% (HPLC)

CH3

HO

HCH3

OH H

OH

C OHO

H

CAS No. 50-24-8C21H28O5=360.44

Wako Cat. #162-21911 (200 mg)

5-Propylsulfonyl-1H-benzimidazole-2-amine Hydrobromide Standard, 98.0+% (HPLC)

[Synonym: Albendazole Metabolite]

CAS No. 80983-34-2C10H13N3O2S・HBr=320.21

Wako Cat. #164-22211 (100 mg)

Roxarson Standard, 98.0+% (HPLC)

O

OHOH

As

HO

O2N

CAS No. 121-19-7C6H6AsNO6=263.04

Wako Cat. #181-01941 (200 mg)

Sulfanilamide Standard,

98.0+% (HPLC)

CAS No. 63-74-1C6H8N2O2S=172.20

Wako Cat. #198-14061 (200 mg)

Sulfi sozole Sodium Standard, 98.0+% (HPLC)

CAS No. 73247-57-1C9H8N3NaO3S=261.23

Wako Cat. #195-14071 (200 mg)

β-Trenbolone Standard, 98.0+% (HPLC)

O

H

CH3

H

OH

CAS No. 10161-33-8C18H22O2=270.37

Wako Cat. #205-16281 (200 mg)

Trenbolone Acetate Standard, 98.0+% (HPLC)

O

H

CH3

H

O CH3

O

CAS No. 10161-34-9C20H24O3=312.40

Wako Cat. #208-16271 (200 mg)

1. Food Analysis


21

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urity

So

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an

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nts


A. LC/MS Solvents Ultrapure water now available!

Liquid chromatography - mass spectrometry (LC/MS) is widely used in various fi elds including biological, food, and environmental analyses. In particular, recent breakthroughs in the development and upgrades of device interfaces have led to the use of LC/MS in microanalyses of environmental pollutants and chemical metabolites, etc. In addition to LC/MS solvents already available, we now off er ultrapure water. Th is water guarantees a total organic carbon (TOC) level of 4ppb or less, and is an ideal LC/MS reagent to analyze trace components.

Description Wako Cat. No. Package Size Features Specifi cation

Ultrapure Water

214-01301 1 L . Decreased total organic carbon levels. Guarantees the absorbance and fl uorescence tests. Use of specially processed glass containers / aluminum

caps

Density (20 C̊): 0.997 ~ 0.999 g/mLRefractive index nD20: 1.332 ~ 1.334Absorbance (210~400nm): max. 0.01Fluorescence test: to pass testTotal organic carbon (TOC): max. 4 ppb

210-01303 3 L

Acetonitrile

016-19852 100 mL

. Suitability test for LC/MS analysis performed

. Guarantees noise level at m/z 50~2,000

. Use of aluminum caps Reduced risks of slight amounts of contaminants from plastic caps

Assay (cGC): 99.8+%Density (20 C̊): 0.780 ~ 0.783 g/mLFluorescence test: to pass testSuitability for LC/MS analysis: to pass test

012-19851 1 L

018-19853 3 L

Methanol

132-14524 100 mLAssay (cGC): 99.7+%Density (20 C̊): 0.789~0.792 g/mLFluorescence test: to pass testSuitability for LC/MS analysis: to pass test

138-14521 1 L

134-14523 3 L

Acetic Acid 018-20061 50 mL

. Suitability test for LC/MS analysis performed

. Reduced background noise

Assay (HPLC): 99.5+%Absorbance (1→ 4, 250nm): max. 0.50Absorbance (1→ 4, 254nm): max. 0.10Fluorescence test: to pass testSuitability for LC/MS analysis: to pass test

Formic Acid (abt. 99%) 067-04531 50 mL

Assay (HPLC): 99.5+%Solubility in water: to pass testAbsorbance (1→ 4, 254nm): max.1.00Fluorescence test: to pass testSuitability for LC/MS analysis: to pass test

RelatedProducts

Description Wako Cat. No. Package Size Features Joint Type

Wakopak® MS-5C18GT 001-00030

2.0mmo x 50mm . The glass lined inside wall of the stainless column allows maximum inactivation

. Excellent peak shapes and recovery rates in analyses of trace components within biological samples

DuPont2.0mmo x 100mm

2.0mmo x 150mm

B. HPLC Solvents Ready-to-Use Acid Solution in Acetonitrile for HPLC analysis

Acetonitrile to which acid is added is commonly used as a solvent in HPLC analyses. In particular, the widespread use of LC/MS has led to an increase in the number of cases that use acetic acid and formic acid. Th ese products consist of acetonitrile for LC/MS to which high-purity acid has been added, respectively. Th ese solutions meet the specifi cations for UV and fl uorescent substance and are thus ideal as solvents for HPLC analyses.

Description Wako Cat. No. (Pkg. Size) Specifi cations Grade

0.1 vol% Acetic Acid-Acetonitrile 011-20551 (1 L); 017-20553 (3 L) . Fluorescence test : to pass test. Suitability for LC/MS analysis : to pass test

for LC/MS0.1 vol% Formic Acid-Acetonitrile 062-04721 (1 L); 068-04723 (3 L)

0.1 vol% Trifl uoroacetic Acid-Acetonitile 206-16451 (1 L); 202-16453 (3 L)

. Absorbance (240 nm) : max. 0.30

. Absorbance (270~400 nm) : max. 0.01

. Fluorescence test : to pass testfor HPLC

RelatedProducts

Description Wako Cat. No. Pkg. Size Content (volume ratio) Specifi cations Grade

Acetonitrile Solution (1+9) 010-19911 50 mL CH3CN : H2O = 1 : 9

. Absorbance (200 nm) : max. 0.05

. Absorbance (225~400 nm) : max. 0.01

. Fluorescence test : to pass testfor HPLC









1 3

2.High Purity Solvents and Reagents



Ch

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A. Wakopak® Fluofi x®-II 120EFluorocarbon Chains bonded Highly Purifi ed Spherical Silica-Gel

Wakopak® Fluofi x®-II 120E is a reversed-phase HPLC column packed with highly purifi ed spherical 5µm silica-gel (SiO2 > 99.99%), to which

fl uorocarbon chains are bonded.

Fluofi x®-II 120E can provide the best separation for fl uorine compounds, isomers in particular. Th e end-capping effi ciency of Fluofi x®-II 120E

is remarkably improved and its non-specifi c adsorption is minimized compared with Fluofi x 120E. Because of its high retention capacity,

Fluofi x®-II 120E has higher selectivity and versatility.

Th e separation mode of this column is basically reversed phase like hydrocarbon modifi ed silica-gel column such as ODS, C8, etc., its

retention capacity is comparable as C4. Because of its strong hydrophobic/lipophobic properties as well as its characteristic fl uorinated phases,

this column specifi cally shows highly retaining and separating capabilities depending on the characteristics of the specimens.

[Features]

1. Improvement in separation capability

2. High recognition capability of halogen compounds such as fl uorides

3. Basic compounds are also applicable

4. Recognition of specifi c compounds by adamant branched fl uorocarbon chain

5. High durability by chemically stable fl uorocarbon

High Purity Spherical Silicagel (99.99+%)

Schematic Surface Model of fl uofi x

Separation of Low Molecular Compounds Recognition of Planar Structures and Fluorinated Compounds

Separation of Fluorinated Regioisomers Separation of Basic Compounds Separation of Catechins

Description Particle Size Pore Size Surface Area End-capping Purpose Size*(I.D.xlength(mm))

Wakopak® Fluofi x®-II 120 E

5μm 12 nm 300 m2/g

Yes for Ordinary analysis

2.0; 4.6 x 50; 150; 250

Wakopak® Fluofi x® 120 E

4.6 x 150; 250

Wakopak® Fluofi x® 120 N No for acidic compounds

* : Available in a variety of inside diameters and lengths. Please contact us for details.



41

Fluofi x®-II 120E

Fluofi x® 120E

ODS Column

Fluofi x®-II 120E

Fluofi x® 120E

ODS Column

Fluofi x®-II 120E

Fluofi x® 120E

ODS Column

Fluofi x®-II 120E

Fluofi x® 120E

ODS Column

Fluofi x®-II 120E

Fluofi x® 120E

ODS Column

Ch

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Pro

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ctsE

nv

iron

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nta

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B. Syringe-type solid-phase extraction columnSample Pretreatment

Presep Polymer Column-Ion Exchange

Presep series are solid-phase extraction columns. We have launched the 4 kinds of syringe-type ion-exchange columns, in which polymer series

ion-exchange resin are fi lled. Th ese are applicable to sample pretreatment in environmental analyses, food analysis and so on.

Description Wako Cat. No. Pkg. Size

Sorbent

Weight

Column

SizeFunctional Mode ion-exchange group Type exchange

capacity Particle Size General Application

Presep DEA 292-61701 5 x 10 pieces

250 mg

6 mL

(Syringe-

type)

Anion Exchange

(weak)diethylaminoethyl

Cl

0.114±0.013

45~90um

Ionic, acidic

analytesPresep QA 296-61601 5 x 10 pieces

Anion Exchange

(strong)trimethylaminoethyl 0.110±0.013

Presep CM 298-61801 5 x 10 piecesCation Exchange

(weak)carboxymethyl

Na

0.103±0.013Ionic, basic

analytesPresep S 294-61901 5 x 10 pieces

Cation Exchanger

(strong)sulfonylpropyl 0.125±0.013

RelatedProducts

Presep RPP series are solid-phase extraction column, in which hydrophilic reverse phase polymer are fi lled. Compared with silica system

fi llers, it has advantages such as high retention of polar compounds are low absorption due to interaction with basic compounds.

Description Wako Cat. No. Pkg. Size

Sorbent

Weight

Column

SizeFunctional Mode Filler

Pore

SizePore Volume Particle Size General

Application

Presep RPP (Syringe-type)

294-36851 5 x 10 pieces 60 mg 3 mL

Reverse Phase

Polymer (RPP)

styrene vinyl

benzene poly -

methacrylate

9 nm 1.2 mL/g 60 μm

polar

compounds

and low

absorption

290-36951 5 x 10 pieces 200 mg 6 mL

290-37051 5 x 10 pieces 500 mg 6 mL

Presep-C RPP (Long cartridge)

293-41951 3 x 10 pieces 360 mg cartridge

Presep-C RPP (Short cartridge)

297-41851 3 x 10 pieces 190 mg cartridge



A. Solvents for PCB AnalysisEthyl Acetate 300 and 5000, suitable for Pesticide Residue-PCB Analysis

Ethyl Acetate 300 and 5000, suitable for pesticide residue-PCB analysis are now also availabe in 3L package sizes. Th is product ensures safety as it contains no interfering substances in the 300- or 5000-fold concentrated solution, and is ideal for the extraction of pesticides from the test substances and for purifi cation.

Description Wako Cat. No. Pkg. Size Specifi cation

Ethyl Acetate 300052-04421 1 L Appearance : Colorless, clear liquid

Density (20C) : 0.899 ~ 0.902 g/mL

Water : max. 0.05 %

Residue after evaporation : max. 2 ppm

Suitability for pesticide residue-PCB analysis : to pass test

Assay (cGC) : min. 99.7%

058-04423 3 L

Ethyl Acetate 5000052-06981 1 L

058-06983 3 L

Suitability for Pesticideresidue-PCB Analysis

The 300- and 5000-fold concentrated solution guarantee the followings :

1 With the GC-ECD, the level of impurities does not exceed 1/2 of the peak of the organochlorine pesticide γ-BHC (20pg).

2 With GC-FPC, the level of impurities does not exceed 1/25 of the peak of the organophosphorous pesticide MPP (0.5ng)

1 5





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B. Solvents for Dioxins AnalysisDimethyl Sulfoxide is available as a solvent used in the analysis of dioxins.

Th is product is used to determine Dibenzo-p-dioxins, Dibenzofurans, and Coplanar PCBs by high resolution GC/MS, and ensures a

suffi ciently low concentration.

It can be used for the preparation of standard solutions, with the ELISA kit, and in the process of analyzing dioxins such as during distribution

of DMSO.

Description Wako Cat. No. Pkg. Size Density (20�) Suitability for dioxins determination (unit)

Dimethyl Sulfoxide,

99.0+% (cGC)041-29395 500 mL 1.100 ~ 1.106 g/mL

Dibenzo-p-dioxin(Cl: 4 ~ 7) : ≦ 5

fg/μL

(Cl: 8) : ≦ 10

Dibenzofuran(Cl: 4 ~ 7) : ≦ 5

(Cl: 8) : ≦ 10

Coplanar PCB - : ≦ 5

Other solvents for Dioxins Analysis

Description Wako Cat. No. Pkg. SizeSpecifi cations

Suitability for dioxins determination (unit)

Acetone, 99.8+% (cGC)010-17831

016-17833

1 L

3 LDensity (20c): 0.789~0.792g/mL


pg/L

(Cl: 7 or 8) : ≦ 5


(Cl: 7 or 8) : ≦ 5


Decane, 99.5+% (cGC)048-28543

042-28541

2 mL x 5

100 mLDensity (20c): 0.727~0.735g/mL


fg/μL

(Cl: 7 or 8) : ≦ 50


(Cl: 7 or 8) : ≦ 50


Dichloromethane, [Methylene Chloride]

99.5+% (cGC, except MeOH)

Stabilizer: Methanol [0.2~0.5%]

048-26321

044-26323

1 L

3 LDensity (20c): 1.320~1.331g/mL


pg/L(Cl: 7 or 8) : ≦ 5


(Cl: 7 or 8) : ≦ 5

Diethylene glycol, 99.0+% (cGC) 040-28645 500 mLSolubility in water: to pass test

Density (20c): 1.116~1.123g/mL


pg/L

(Cl: 7 or 8) : ≦ 25


(Cl: 7 or 8) : ≦ 25

Coplanar PCB(2,3',4,4',5-PeCB) : ≦ 100

(Other Co-PCB) : ≦ 50

Diethyl ether, 99.5+% (cGC) 049-27451 1 L Density (20c): 0.712~0.714g/mL


pg/L

(Cl: 7 or 8) : ≦ 5


(Cl: 7 or 8) : ≦ 5

Ethanol (99.5), (cGC)[Ethyl Alcohol (99.5)]

050-06661

056-06663

1 L

3 LDensity (20c): 0.789~0.792g/mL Coplanar PCB - : ≦ 10

Fluorobenzene, 98.0+% (cGC) 061-04551 200 mL Density (20c): 1.025~1.035g/mL

Dibenzo-p-dioxin(Cl: 4 ~ 7 : ≦ 5

fg/μL

(Cl: 8) : ≦ 10


(Cl: 8) : ≦ 10


Hexane, 96.0+% (cGC)[n-Hexane]

083-07391

089-07393

1 L

3 LDensity (20c): 0.658~0.662g/mL


pg/L

(Cl: 7 or 8) : ≦ 5


(Cl: 7 or 8) : ≦ 5

Methanol, 99.8+% (cGC)[Methyl Alcohol]

136-13461

132-13463

1 L

3 LDensity (20c): 0.791~0.793g/mL Coplanar PCB - : ≦ 10

Nonane, 98.0+% (cGC)[n-Nonane]

148-07351

142-07354

2 mL x 5

100 mLDensity (20c): 0.713~0.721g/mL


fg/μL

(Cl: 7 or 8) : ≦ 10


(Cl: 7 or 8) : ≦ 10


Petroleum ether Content: 5~15 %

160-20231 1 LBoiling range (30~60c): 90+vol%


pg/L

(Cl: 7 or 8) : ≦ 5


(7, 8 chlorinated) : ≦ 5


2% Sodium Chloride Solution 195-12631 1 L

-

Dibenzo-p-dioxin

(Cl: 4) : ≦ 1

pg/L

(Cl: 5 or 6) : ≦ 2

(Cl: 7 or 8) : ≦ 5

Dibenzofuran

(Cl: 4) : ≦ 1

10% Sodium Chloride Solution 192-12641 1 L

(Cl: 5 or 6) : ≦ 2

(Cl: 7 or 8) : ≦ 5


Toluene, 99.7+% (cGC)203-14141

209-14143

1 L

3 LDensity (20c): 0.864~0.868g/mL


pg/L

(Cl: 7 or 8) : ≦ 5


(Cl: 7 or 8) : ≦ 5


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Wako Cat.#(Pkg. Size) Description

012-20581

(0.35mg)

Aconitum Diester Alkaloids Standardfor the JP Crude Drugs Test (for Purity Test)

<Contents>

aconitine: 0.05 mg; jesaconitine: 0.05 mg; mesaconitine: 0.1 mg; hypaconitine: 0.15 mg

Source: Aconitum carmichaeli Debeaux, Aconitum japonicum Thunberg (Ranunculaceae)

Active ingredient contained in Powdered Processed Aconite Root.

This product is used for the preparation of standard solutions of mixtures of diester alkaloids in aconite roots for reagents and test solutions and of standard solutions of mixtures for purity tests as described in the general test procedures in the guidelines of Supplement 2 to the Japanese Pharmacopoeia (14th ed.). To be used after the product has been accurately dissolved in 5mL of acetonitrile-phosphate buff er (1:1).

018-20681

(20mg)

Alisol Afor the JP Crude Drugs Test (for TLC)

Source: Alisma orientale Juzepczuk (Alismataceae)

Active ingredient contained in Alisma Rhizome.

Used to verify the ingredients of Saireito extract.

OH

O

OH

H

HO

OH

H

011-20291

(20mg)

Arbutinfor the JP Crude Drugs Test (for Ingredient Content Determination and TLC)

Source: Arctostaphylos uva-ursi (Linné) Sprengel (Ericaceae)

Active ingredient contained in Bearberry Leaf.

Has antibacterial properties that prevent coliform growth, and medicinal properties including

enhanced therapeutic eff ects against contact dermatitis to picryl chloride in the mouse.

OCH2OH

HH

OHH

OH

OH

HH

O OH

nH2O

CAS No. 497-76-7C12H16O7 • nH2O (C12H16O7=272.25)

015-20691

(20mg)

Astragaloside IVfor the JP Crude Drugs Test (for TLC)

Source: Astragalus membranaceus Bunge, Astragalus mongholicus Bunge (Leguminosae)

Active ingredient contained in Astragali Radix.

Used to verify the ingredients of Hochuekkito extract. O

H

O

H

OH

OHO

OH

OH

OH

O

OH

OH

OH

O

OH

028-15231

(10mg)

Barbaloinfor the JP Crude Drugs Test (for Ingredient Content Determination and TLC)

Source: Aloe ferox Miller, Aloe africana Miller/ Aloe spicata Baker

Active ingredient contained in Aloe.

Has cathartic properties when powdered aloe is administered orally to rats.

OH O OH

HCH2OH

Glc

022-15491

(5mg)

Benzoylmesaconine Hydrochloridefor the JP Crude Drugs Test (for TLC)

Source: Aconitum carmichaeli Debeaux Aconitum japonicum Thunberg (Ranunculaceae)

Active ingredient contained in Aconite Tuber.

025-15241

(20mg)

Bufalinfor the JP Crude Drugs Test (for Ingredient Content Determination)

Source: Bufo bufo gargarizans Cantor

Bufalin is an active ingredient contained in the venom of Bufo bufo gargarizans Cantor. Has

local anesthetic properties on the cornea of guinea pigs and rabbits, and is more eff ective than

Cinobufagin or Resibufogenin.

O

O

H

H

HOH

OH

H3C

H3C

H2O

5. Standards for Crude Drug Test


CAS No. 19885-10-0C30H50O5=490.72

CAS No. 84687-43-4C41H68O14=784.97

CAS No. 1415-73-2C21H22O9=418.40

CAS No. 126266-38-4C31H43NO10 • HCl • xH2O

CAS No. 465-21-4C24H34O4 • nH2O (C24H34O4=386.52)

1 7


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030-19491

(20mg)

Cinobufaginfor the JP Crude Drugs Test (for Ingredient Content Determination)


Cinobufagin is an active ingredient contained in the venom of Bufo bufo gargarizans Cantor. Has

cardiotonic properties, and properties of restoring the contractile properties of the heart in cases

of heart failure induced by Pentobarbital in studies using dog heart-lung preparation.

OO

O

H

H

HOH

OO

H3C

H3C

H2O

CAS No. 470-37-1C26H34O6 • nH2O (C26H34O4=442.52)

085-08211

(10mg)

Epihesperidin Standard

[(2R)-Hesperidin Standard]for Crude Drugs Determination

Source: Citrus natsudaidai, Citrus unshiu, Citrus aurantium var daidai

Flavonoid and a type of vitamin P contained in citrus. Hesperidin (S) and Epihesperidin (R) after

chiral resolution are available.

071-05071

(20mg)

Geniposidefor the JP Crude Drugs Test (for Ingredient Content Determination and TLC)

Source: Gardenia jasminoides Ellis (Rubiaceae)

Geniposide is an active ingredient contained in gardenia (gardenia fruit). As genipin, has

choleretic properties in the gastrointestinal tract of rats.

O

COOCH3H

HOGlcOH

CAS No. 24512-63-8C17H24O10=388.37

074-05061

(20mg)

[6]-Gingerolfor the JP Crude Drugs Test (for TLC)

Source: Zingiber offi cinale Roscoe (Zingiberaceae)

Active ingredient contained in ginger. Has numerous medicinal properties, among which the

inhibition of constriction and enhancement of isolated vessels in mice and rats have been

reported.

OH

OCH3

O OH

CAS No. 23513-14-6C17H26O4=294.39

076-05021

(10mg)

Ginsenoside Rg1

for the JP Crude Drugs Test (for TLC)

Source: Panax ginseng C. A. Meyer (Panax Schinseng Nees) (Araliaceae)

Active ingredient contained in ginseng roots. Stimulates the cerebral cortex of rats and enhances

the secretion of neurotransmitters at the synapse.

R2O

OH

OR1

OH

R1=R2=Glc-

CAS No. 22427-39-0C42H72O14=801.01

070-05161

(20mg)

Glycyrrhizic Acidfor the JP Crude Drugs Test (for TLC)

Source: Glycyrrhiza uralensis Fisher, Glycyrrhiza glabra Linné (Leguminosae)

Active ingredient contained in Glycyrrhiza.

O

H

H H

RO

CH3

CH3

CH3CH3

CH3H3C

H3C

R=GlcA GlcA

COOH

H2O

2

• n

086-08361

(10 mg)

Hesperidinfor the JP Crude Drugs Test (for TLC)

Source: Citrus natsudaidai, Citrus unshiu, Citrus aurantium var daidai

Flavonoid and a type of vitamin P contained in citrus. Hesperidin (S) and Epihesperidin (R) after

chiral resolution are available.

C28H34O15=610.56

094-05281

(20mg)

Icariinfor the JP Crude Drugs Test (for TLC)

Source: Epimedium sagittatum Maximowicz

Active ingredient contained in Epimedii herba.

OH O

OR1

R2 O

O-CH3

O



CAS No. 1405-86-3C42H62O16=822.93

CAS No. 489-32-7C33H40O15=676.66

81

CAS No. 520-26-3C28H34O15=610.56

R1=A-L-RhaR2=B-D-Glc

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128-05311

(20mg)

(Z)-Ligustilidefor the JP Crude Drugs Test (for TLC)

Source: Angelica acutiloba Kitagawa, Angelica acutiloba Kitagawa var. sugiyamae Hikino

(Umbelliferae)

Active ingredient contained in Angelica Root.

Used to verify the ingredients of Hochuekkito extract.

O

O

123-05121

(20mg)

Loganinfor the JP Crude Drugs Test (for TLC)

Source: Cornus offi cinalis Siebold et Zuccarini (Cornaceae)

Active ingredient contained in the pseudocarp of the Cornus Fruit

H

H

H

H

H

H

HOH

OH

OH

O

HO

CH2OH

COOCH3

H3C

O

O

167-21721

(10mg)

Paeonolfor the JP Crude Drugs Test (for Ingredient Content Determination and TLC)

Source: Paeonia suff ruticosa Andrews (Paeonia moutan Slims) (Paeoniaceae)

Active ingredient contained in the peony root bark. Numerous medicinal properties have been

reported, such as that the administration of its infused extract prevents adjuvant arthritis.

COCH3

OH

OCH3

187-01921

(20mg)

Resibufogeninfor the JP Crude Drugs Test (for Ingredient Content Determination and TLC)


Resibufogenin is an active ingredient contained in the venom of Bufo bufo gargarizans Cantor.

Has local anesthetic properties, anti-infl ammatory properties, and cardiotonic properties.

O

O

H

H

HOH

O

H3C

H3C

H2O

190-13541

(10mg)

Saikosaponin afor the JP Crude Drugs Test (for TLC)

Source: Bupleurum falcatum Linné (Umbelliferae)

Active ingredient contained in Bupleuri Radix. Has central depressant properties such as

antitussive properties in guinea pigs.

RO

O

OH

CH2OH R=Glc-3Fuc-

197-13551

(20mg)

Schizandrinfor the JP Crude Drugs Test (for TLC)

Source: Schisandra chinensis Baillon (Schisandraceae)

Active ingredient contained in Schisandra Fruit. Has antitussive properties and prevents hepatic

disorders in mice, rats, and rabbits.

OH

OCH3

CH3O

CH3O

CH3O

CH3O

OCH3

194-13941

(10mg)

Sennoside Bfor the JP Crude Drugs Test (for TLC)

Source: Cassia angustifolia Vahl, Cassia acutifolia Delile (Leguminosae)

Active ingredient contained in Senna Leaf. Used to determine the ingredients contained in

powdered senna.

OR1O OH

HH

OR2 O OH

R1=R2=Glc-

COOH

COOH

1 9



CAS No. 4431-01-0C12H14O2=190.24

CAS No. 18524-94-2C17H26O10=390.38

CAS No. 552-41-0C9H10O3=166.18

CAS No. 465-39-4C24H32O6 • nH2O (C24H32O6=442.54)

CAS No. 20736-09-8C42H68O=780.98

CAS No. 7432-28-2C24H32O7=432.51

CAS No. 128-57-4C42H38O20=862.74

Wako Chemicals USA, Inc.http://www.wakousa. comToll-Free (U.S. only): 1-877-714-1920Head Offi ce (Richmond, VA): Tel: 1-804-714-1920/ Fax: 1-804-271-7791Los Angeles Sales Offi ce (Irvine, CA):Tel: 1-949-679-1700 / Fax: 1-949-679-1701Boston Sales Offi ce (Cambride, MA):Tel: 1-617-354-6772 / Fax: 1-617-354-6774

Wako Chemicals GmbHhttp://www.wako-chemicals.de

European Offi ce: Nissanstraße 2, D-41468Neuss, GermanyTel: 49-2131-311-0Fax: 49-2131-311100

Listed products are intended for laboratory research use only, but not to be used for drug, food or human use. Please visit our online catalog to search for other products from Wako ; http://www.e-reagent.comThis brochure may contain products that cannot be exported to your country due to regulations.Bulk quote requests for some products are welcomed. Please contact us.

Wako Product U

pdate No. 16

Wako Pure Chemical Industries, Ltd.http://www.wako-chem.co.jp

1-2, Doshomachi 3-ChomeChuo-Ku, Osaka 540-8605, JapanTel: 81-6-6203-3741Fax: 81-6-6201-5964


198-13581

(10mg)

Shikonin (mixture of optical isomers about 6:1)for Crude Drugs Determination

Active ingredient contained in Shikon. Enhances wound healing and granulation of wounds,

prevents edema, and has anti-infl ammatory and antibacterial properties.

OOH

OH O

HO H

C16H16O5=288.30

198-13601

(10mg)

Shikonin (mixture of optical isomers about 1:1)for Crude Drugs Determination

Assay (HPLC): 98.0+%

199-14111

(5mg)

[6]-Shogaolfor the JP Crude Drugs Test (for TLC)

Source: Zingiber offi cinale Roscoe (Zingiberaceae)

Active ingredient contained in Ginger.

02

CAS No. 555-66-8C17H24O3=276.37



Alphabetical Index -Wako Product Update No.16-

page DescriptionA 6 Abamectin Std

13 Acetic Acid13 0.1% Acetic Acid-Acetonitrile13 Acetonitrile16 Acetone, for Dioxins Analysis13 Acetonitrile Solution6 Acifl uorfen Std

17 Aconitum Diester Alkaloids Std17 Aconitine6 Agrimec9 Alanap

17 Alisol A6 4-Aminopyridine Std

11 Amoxicillin Trihydrate Std11 Ampicillin Std6 Anilofos Std

17 Arbutin6 Arozin3 LD-Aspartame3 L-A-Aspartyl-D-Phenylalanine Methyl Ester

17 Astragaloside IV6 Avitrol-200

B 6 BA17 Barbaloin7 Basagran6 6-Benzylaminopurine Std

17 Benzoylmesaconine Hydrochloride11 Benzylpenicillin Potassium Std11 Betamethasone Std11 Bithionol Std6 Bromochloromethane Std

17 Bufalin6 Butamifos Oxon Std3 Butenolide

11 Butylhydroxsyanisole StdC 6 Carboxin Std

11 Chloramphenicol Std11 Chlormadinone Acetate Std7 Chloroxuron Std

11 Chlorpromazine Hydrochloride Std18 Cinobufagin11 Ciprofl oxacin Hydrochloride Monohydrate Std3 Citrinin Std7 4-CPA Std7 Cyanamide Std

D 16 Decane, for Dioxins Analysis3 Deoxynivalenol Std

11 Dexamethasone Std3 Diacetoxyscirpenol

11 Dibutylhydroxytoluene Std7 Dichloroprop Std

16 Dichloromethane, for Dioxins Analysis7 1,1-Dichloro-2,2-bis(4-ethylphenyl)ethane Std

16 Diethylene glycol, for Dioxins Analysis16 Diethyl ether, for Dioxins Analysis7 2,6-Difl uorobenzoic Acid Std7 2,6-Diisopropylnaphthalene Std

16 Dimethyl Sulfoxide, 99.0+%

page DescriptionD 11 Dinitolmide Std

16 DMSO7 DNOC Std

10 DroppE 18 Epihesperidin Std

10 Erbiotox16 Ethanol (99.5), for Dioxins Analysis15 Ethyl Acetate, for PCB Analysis7 Ethylan

11 Eugenol StdF 7 Fenbendazole Std

9 Fenthion Oxon8 Flufenpyr-ethyl Std

14 Fluofi x®-II 120E, Fluofi x® 120E, 120N16 Fluorobenzene, for Dioxins Analysis8 Fluridone Std8 Forchlorfenuron Std

13 Formic Acid (abt. 99%)13 0.1% Formic Acid-Acetonitrile8 Frastar2 Fructooligosaccharides Set2 1F-Fructofranosylnystose3 Fumonisin B1

G 18 Geniposide8 Gibberellin Acid8 Gibberelline A3 Std

18 [6]-Gingerol18 Ginsenoside Rg118 Glycyrrhizic Acid

H 18 Hesperidin16 Hexane, for Dioxins Analysis8 Hexazinone Std

11 Hydrocortisone Std17 Hypaconitine

I 18 Icariin12 Isocyanuric Acid Std

J 17 JesaconitineK 2 1-Kestose

8 Kresoxim-methyl StdL 19 (Z)-Ligustilide

19 LoganinM 12 Meloxicam Std

8 Mepiquat Chloride Std17 Mesaconitine

13,16 Methanol8 Methyl Isothiocyanate Std

16 Methylene Chloride, for Dioxins Analysis8 1-Methylpiperidine Std

12 Metoclopramide Std12 Metronidazole Std9 MPP Oxon Std9 MPP Oxon Sulfoxide Std3 Mycotoxin Analysis

N 9 Naproanilide Std9 Naptalam Std3 Neosolaniol Std9 Nitrapyrin Std

12 Nitroxynil Std

page DescriptionN 3 Nivalenol Std

16 Nonane, for Dioxins Analysis12 Norfl oxacin Std9 N-Serve2 Nystose2 Nystose Trihydrate

O 3 Ochratoxin A12 Oxacillin Sodium Monohydrate Std9 Oxfendazole Std

12 Oxyclozanide StdP 19 Paeonol

2 Para Red Std3 Patulin5 Pesticide Mixture PL-1-1, etc.

16 Petroleum ether, for Dioxins Analysis10 Phenmedipharm Metabolite

4, 11 Positive List System12 Potassium Clavulanate Std12 Prednisolone Std15 Presep CM, DEA,QA, S and RPP15 Presep-C RPP12 5-Propylsulfonyl-1H-benzimidazole-2-amine Hydrobromide Std9 Pyridalyl Std9 Pyrimethanil Std

R 19 Resibufogenin12 Roxarson Std

S 19 Saikosaponin a10 Sakarat19 Schizandrin19 Sennoside B20 Shikonin20 [6]-Shogaol16 Sodium Chloride Solution, for Dioxins Analysis15 Solid-phase Extraction Syringe-type column8 Sonar2 Sudan I, II Std

12 Sulfanilamide Std12 Sulfi sozole Sodium Std10 Sulprofos Oxon Std

T 3 T-2 Toxin Std10 TCA-sodium Std10 Tecnazene Std10 Thidiazuron Std10 Tiadinil Std10 Tolclofos-methyl Oxon Std16 Toluene, for Dioxins Analysis10 m-Toluidine Std7 Tomatotone8 Trapex

12 B-Trenbolone Std12 Trenbolone Acetate Std13 0.1% Trifl uoroacetic Acid-Acetonitile

U 13 Ultrapure Water9 Uribest

V 10 VGETW 13 Wakopak® MS-5C18GT

14 Wakopak® Fluofi x® - II 120 E, Fluofi x® 120E, N10 Warfarin Std

06916IBK

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wako product update

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