Verticillium dahliaeVerticillium dahliaeSpinach SeedSpinach Seed

Progress ReportProgress Report

June 2, 2009June 2, 2009

Seed Industry CollaboratorsSeed Industry Collaborators

California Seed AssociationCalifornia Seed AssociationAmerican Seed Trade Association (ASTA)American Seed Trade Association (ASTA)ASTA - VegTech SubcommitteeASTA - VegTech SubcommitteePuget Sound Seed Growers AssociationPuget Sound Seed Growers AssociationResearchersResearchersNational Seed Health SystemNational Seed Health SystemInternational Seed Health Initiative (ISHI International Seed Health Initiative (ISHI Veg)Veg)

Seed Industry PresentersSeed Industry Presenters

Betsy Peterson – CSABetsy Peterson – CSA

Philip Brown – Alf Christianson SeedPhilip Brown – Alf Christianson Seed

Tom Moore - Enza CoastalTom Moore - Enza Coastal

Samantha Thomas - Seminis Samantha Thomas - Seminis

Todd Rehrman – Syngenta SeedTodd Rehrman – Syngenta Seed

Progress HighlightsProgress Highlights

Communication EstablishedCommunication Established

Production PracticesProduction Practices

Information Sheet Information Sheet

Research ActivitiesResearch Activities

Seed TestingSeed Testing

Product RegistrationsProduct Registrations

Seed Treatments/ProcessesSeed Treatments/Processes

Communications Communications

Meetings Leafy Greens/ Seed IndustryMeetings Leafy Greens/ Seed Industry– August 2008 – SalinasAugust 2008 – Salinas– March 16, 2009 – prior to CLGRB meetingMarch 16, 2009 – prior to CLGRB meeting

Industry ConferencesIndustry Conferences

Industry / Researcher Roundtables Industry / Researcher Roundtables

Conference CallsConference Calls

SPINACHSPINACH“A Challenging Crop”

Research and DevelopmentFrom Breeding to Sales

• Year• 1-5 Selecting Lines: male – female• 6 Control on sex expression and purity• 6 Trial combinations• 6 Parent line propagation (trial)• 6 Trial seed production • 7 1st screening trial• 7 Parent line propagation (commercial)• 7 Seed production• 8 2nd screening• 9 Commercial

• Disease resistances• Good leaf texture / high yield• Early – late maturity / vigor• Process ability: harvest to shelf life• Field stand ability: holding

Product Development Specifications(Depending on market and type)

• Stands erect

• Long Standing (Bolt resistance)

• Uniformity / purity• Reliability / consistency

Product Development Specifications(Depending on market and type)

Spinach: Spinacia oleracia L.

•Wind pollinated•Hybrid not OP•Isolation required•Hybrid seed production is based on:

Monoecious (female)

Dioecious (male)

• Cool Climate • Very long days +/- 16 hours.• Good rotation 10-15 years (PNW), 5-6 years (EU).

(Fusarium/Verticillium)

Seed Production - Crop

• Grower Experience/Knowledge• Combines.• Seeds dryers• Close proximity of a seed conditioning facility.

Seed Production - Grower

World WideSpinach Seed Production

Locations

Washington 48˚N

Oregon 44˚N

Chile 40˚S Argentina 40˚S

New Zealand 46˚S

Denmark 55˚NFrance 48˚N

Italy 44˚N

Information SheetInformation Sheet

““Fact Sheet” Developed compiled and Fact Sheet” Developed compiled and distributed November 2008distributed November 2008

Literature Search Literature Search

References DocumentedReferences Documented

Peer Reviewed, Version 3 out for review Peer Reviewed, Version 3 out for review

Research ActivitiesResearch Activities

Seed Industry $$ FundingSeed Industry $$ Funding– 2002 – present >$100,000 – Fusarium and 2002 – present >$100,000 – Fusarium and

Verticillium, duToit Verticillium, duToit

Seed Company/Researcher PartnershipsSeed Company/Researcher Partnerships– Trial FundingTrial Funding– Seed SamplesSeed Samples– Technical AssistanceTechnical Assistance– Ring TestsRing Tests– Industry Collaboration Industry Collaboration

Research Activities Continued Research Activities Continued

Recently Funded Research Recently Funded Research – ASTA (April 2009)ASTA (April 2009)

Lindsey duToit (WSU), Jim Correll (UAR)Lindsey duToit (WSU), Jim Correll (UAR)

$90,000 for 3 yrs.$90,000 for 3 yrs.

Project has 4 objectivesProject has 4 objectives

ASTA Funded ProjectASTA Funded Project

Objective 1: Examine the process of infection of spinach plants by V. dahliae from seedborne and soilborne inoculum, particularly with respect to earliness of bolting and development of Verticillium wilt symptoms.

ASTA Funded ProjectASTA Funded Project

Objective 2:Objective 2: Assess the efficacy of conventional and organic seed treatments and soil drenches for protecting spinach against soilborne inoculum of V. dahliae (the efficacy against seedborne inoculum is being evaluated in the CLGRP project).

ASTA Funded ProjectASTA Funded Project

Objective 3:Objective 3: Identify thresholds for soilborne vs. seedborne inoculum of V. dahliae for spinach cultivars with different levels of susceptibility to Verticillium wilt, and the influence of soil pH and soil type on V. dahliae inoculum potential from seedborne vs. soilborne sources.

ASTA Funded ProjectASTA Funded Project

Objective 4:Objective 4: Determine the influence of soil microbial activity and soil fumigation on seed transmission, soil infestation, and development of Verticillium wilt as a result of planting spinach seed infected with V. dahliae

CDFA Specialty Crop Block GrantCDFA Specialty Crop Block Grant

Concept Submitted Concept Submitted

June 4June 4thth Invitations to submit full proposal Invitations to submit full proposal

June 29June 29thth Proposal Due Date Proposal Due Date

Potential Funding SourcesPotential Funding Sources

Specialty Crop Block Grant ProgramSpecialty Crop Block Grant Program

USDA-CSREES- Specialty Crop Research USDA-CSREES- Specialty Crop Research InitiativeInitiative

Technical Assistance for Specialty Crops Technical Assistance for Specialty Crops (TASC) (TASC)

Seed TestingSeed Testing

Partnerships - Seed Industry/Labs/ Partnerships - Seed Industry/Labs/ Researchers Researchers – Provide seed for testing Provide seed for testing – Provide $$ funding to lab/researchersProvide $$ funding to lab/researchers– Coordinate & Participate in Ring TestsCoordinate & Participate in Ring Tests– Coordinate and Facilitate TrainingCoordinate and Facilitate Training

Seed Testing Future Seed Testing Future

International Acceptance (VTS / NSHS / International Acceptance (VTS / NSHS / ISHI)ISHI)– AccreditationAccreditation

Coordinate Facilitate WorkshopsCoordinate Facilitate Workshops

Continuing EducationContinuing Education

Product RegistrationProduct Registration

Types of RegistrationsTypes of Registrations– Section 3Section 3– Section 24 (c) (Special Local Need (SLN)Section 24 (c) (Special Local Need (SLN)– Section 18 (Emergency Exemption)Section 18 (Emergency Exemption)– Research Authorization RAResearch Authorization RA

Section 18 or 24 (c ) SLN Section 18 or 24 (c ) SLN

24 (C) SLN24 (C) SLN Sec 18Sec 18

Emergency Emergency NoNo Yes Yes

ResidueTolerance ResidueTolerance YesYes No No

Expiration Expiration No No 1 year 1 year

**GGrown for Seedrown for Seed YesYes No No

* No part of seed plant enters food or feed * No part of seed plant enters food or feed

Product Registration StatusProduct Registration Status

Topsin 24 (C) “grown for seed” WA State Topsin 24 (C) “grown for seed” WA State (approved February 2009)(approved February 2009)

Topsin Section 18 (Possibly?)Topsin Section 18 (Possibly?)

Mertect 340F Section 18 (Initiated Spring Mertect 340F Section 18 (Initiated Spring 2009)2009)

Product Registration Status Product Registration Status Con. Con.

BASF 595 XGF (new a.i., no residue data)BASF 595 XGF (new a.i., no residue data)

Coronet (US EPA Reg in progress for Coronet (US EPA Reg in progress for leafy greens)leafy greens)

Section 18 Product InformationSection 18 Product InformationRequiredRequired

Authorization from RegistrantAuthorization from Registrant

Description of Pesticide RequestedDescription of Pesticide Requested

Name of PestName of Pest

Description of Proposed UseDescription of Proposed Use

Section 18 Data RequiredSection 18 Data Required

Alternative Methods of ControlAlternative Methods of Control

EfficacyEfficacy

Food Quality Protection ActFood Quality Protection Act

Risk to Endangered or Threatened Risk to Endangered or Threatened SpeciesSpecies

Section 18 Economic Data Section 18 Economic Data RequiredRequired

Identified EmergencyIdentified Emergency

Discussion of Events or CircumstancesDiscussion of Events or Circumstances

Significant Economic LossSignificant Economic Loss

Section 18 Reporting RequiredSection 18 Reporting Required

Interim Use ReportingInterim Use Reporting

Progress Toward RegistrationProgress Toward Registration

Section 18 Submission Section 18 Submission

Submit to CA DPR & WSDASubmit to CA DPR & WSDA

States Submit to US EPAStates Submit to US EPA

US EPA establishes time limited toleranceUS EPA establishes time limited tolerance

Seed Treatments/ProcessesSeed Treatments/Processes

AgriCoatAgriCoat

GTGGTG

IncotecIncotec

SummarySummary

Communication EstablishedCommunication Established

Production Practices Production Practices

Information Sheet Information Sheet

Research ActivitiesResearch Activities

Seed TestingSeed Testing

Product RegistrationsProduct Registrations

Seed Treatments/ProcessesSeed Treatments/Processes

Thank You!Thank You!

Questions?Questions?